FastQCFastQC Report
Mon 6 Jun 2016
ERR1211333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211333_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7429630
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232470.3128957969643172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195040.2625164375614936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116920.1573698824840537No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121750.051.3833051
GTATCAA217600.040.9353181
ATCAACG271750.032.201753
TACCTGG64200.032.0828822
CAACGCA273200.031.937665
TATCAAC283100.031.256892
TCAACGC281150.031.1753924
AACGCAG291050.030.252736
GTGGTAT57750.027.9555031
GTACCTG79150.026.3264881
ACGCAGA333600.026.1971917
ACCTGGG77000.026.0200063
GTACATG288550.025.7954441
CGCAGAG342850.025.3672568
TACATGG296350.024.618942
ACATGGG299000.024.0412923
TGGTATC63500.023.9395082
GCAGAGT375100.022.9844539
CATGGGG210950.021.7536684
GAGTACT216850.020.76427812-13
Produced by FastQC (version 0.11.5)