FastQCFastQC Report
Mon 6 Jun 2016
ERR1211332_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211332_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5761187
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354510.6153419425545464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259900.45112231212074877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158100.27442261464521117No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142090.24663320249802687No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG103400.1794769029368427No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT91320.15850900170398913No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC72940.1266058539672467No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT71670.12440144713233574No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66150.11482008829083311No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA62450.10839780066156506No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA60210.1045097130157379No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58880.10220116097602802No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG58540.10161100481550069No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG71950.069.6950452
ACCTGGG75050.065.8602753
TATCACG4450.059.1975172
GTACCTG99550.050.8470041
CCTGGGG44350.046.9319574
GGTATCA169850.042.099061
GTACCCG3400.041.508471
GTATCAA303750.037.3400841
GTACACG6600.036.3513571
ATCACGC7250.036.2918053
TAACGCA5850.036.142254
TCACGCA7100.035.0732544
ATCAACG360950.030.7331773
CAACGCA364550.030.4296845
TCAACGC369750.030.1033924
AACGCAG380950.029.5017626
GTACATG375450.029.1441881
CATGGGT40350.029.1108574
TATCAAC384750.028.8052862
CATGGGG154000.027.9163684
Produced by FastQC (version 0.11.5)