FastQCFastQC Report
Mon 6 Jun 2016
ERR1211332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211332_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5761187
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305790.5307760362578059No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241350.41892408630374267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155490.2698922982364572No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114910.19945542472410635No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG105070.1823756111370799No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT92620.16076548114129952No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT69060.11987113072358178No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC62600.10865816367356242No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA61770.10721748834051038No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA59630.1035029760360148No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58240.10109027879150599No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG75400.069.6855242
ACCTGGG76650.067.026553
CCTGGGG42950.053.4183654
GTACCTG103150.051.3605881
TATCACG3850.047.5225522
TAACGCA7850.046.619744
GTACCCG3650.042.4254381
GGTATCA164650.041.068371
GTACACG6100.039.2324641
GTATCAA288500.036.8081471
TATAACG10500.033.509492
CTGGGGG39750.032.4520725
GACATAT17800.030.053161
ATCAACG346650.029.9932463
TCACGCA6000.029.7151054
CAACGCA353950.029.5668165
TCAACGC357750.029.154444
AACGCAG371550.028.4437626
CATGGGT39200.028.2469024
GTACATG369250.028.1361