##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211332_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5761187 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11208905387032 33.0 31.0 34.0 30.0 34.0 2 32.22651929194453 33.0 31.0 34.0 30.0 34.0 3 32.28623424304748 34.0 31.0 34.0 30.0 34.0 4 35.94464283141651 37.0 35.0 37.0 35.0 37.0 5 35.86695675734879 37.0 35.0 37.0 35.0 37.0 6 35.89611203385691 37.0 35.0 37.0 35.0 37.0 7 35.82744805888092 37.0 35.0 37.0 35.0 37.0 8 35.80934675441016 37.0 35.0 37.0 35.0 37.0 9 37.41662681666122 39.0 37.0 39.0 34.0 39.0 10-11 37.361530618603425 39.0 37.0 39.0 34.0 39.0 12-13 37.313219914576635 39.0 37.0 39.0 34.0 39.0 14-15 38.514198289345586 40.0 38.0 41.0 34.0 41.0 16-17 38.511555344410795 40.0 38.0 41.0 34.0 41.0 18-19 38.578052665188615 40.0 38.0 41.0 34.0 41.0 20-21 38.57101496618665 40.0 38.0 41.0 34.0 41.0 22-23 38.44028826004086 40.0 38.0 41.0 34.0 41.0 24-25 38.35933853561775 40.0 38.0 41.0 34.0 41.0 26-27 38.260538149516755 40.0 38.0 41.0 34.0 41.0 28-29 38.16528989598845 40.0 38.0 41.0 33.5 41.0 30-31 38.120792815786054 40.0 37.0 41.0 34.0 41.0 32-33 38.000658284482 40.0 37.0 41.0 33.0 41.0 34-35 37.85418031041172 40.0 37.0 41.0 33.0 41.0 36-37 37.68416829726235 40.0 37.0 41.0 33.0 41.0 38-39 37.57464624911498 40.0 37.0 41.0 33.0 41.0 40-41 37.480166326835075 40.0 36.5 41.0 32.5 41.0 42-43 37.37477901689357 40.0 36.0 41.0 32.0 41.0 44-45 37.22033914191641 40.0 36.0 41.0 31.5 41.0 46-47 36.95481200315143 40.0 36.0 41.0 31.0 41.0 48-49 35.38775264541839 39.0 35.0 41.0 23.0 41.0 50-51 36.156371594950826 39.0 35.0 41.0 28.0 41.0 52-53 36.29866119603477 39.0 35.0 40.0 29.0 41.0 54-55 36.167799187910404 39.0 35.0 40.0 28.5 41.0 56-57 36.00540878121123 39.0 35.0 40.0 28.0 41.0 58-59 35.82335680129806 38.5 35.0 40.0 28.0 41.0 60-61 35.61220378717094 38.0 35.0 40.0 28.0 41.0 62-63 35.32367366308367 38.0 34.0 40.0 27.0 41.0 64-65 35.0347748649714 37.5 34.0 40.0 26.0 41.0 66-67 34.71744616170244 37.0 34.0 39.5 26.0 41.0 68-69 34.361115599962304 36.5 33.5 39.0 26.0 40.5 70-71 34.01218872083132 36.0 33.0 39.0 26.0 40.0 72-73 33.62372172609568 35.5 33.0 38.5 25.0 40.0 74-75 33.21814514960198 35.0 32.0 37.5 25.0 39.5 76-77 32.210776182061096 34.5 31.0 36.0 23.5 39.0 78-79 32.6141639735006 35.0 32.0 36.5 25.0 39.0 80-81 32.44592442494923 35.0 32.0 36.0 25.0 38.5 82-83 32.130437182476456 35.0 32.0 36.0 24.0 37.0 84-85 31.78053854179703 34.0 31.0 35.0 23.5 37.0 86-87 31.50671172451094 34.0 31.0 35.0 23.0 36.5 88-89 31.245587324278834 34.0 31.0 35.0 21.5 36.0 90-91 31.006506211306803 34.0 31.0 35.0 20.0 36.0 92-93 30.791918227962398 34.0 30.5 35.0 20.0 35.0 94-95 30.578516024562298 34.0 30.0 35.0 20.0 35.0 96-97 30.381532746637106 34.0 30.0 35.0 19.0 35.0 98-99 30.16594809368278 34.0 29.0 35.0 18.5 35.0 100 27.824169394258508 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2717558660095918 1101 2 -0.2793570540293153 1101 3 -0.3237744908223945 1101 4 -0.21567825341413993 1101 5 -0.2141149335257282 1101 6 -0.2250381073921517 1101 7 -0.26273647698220515 1101 8 -0.2671886777102941 1101 9 -0.3550147353095241 1101 10-11 -0.3378203360462635 1101 12-13 -0.4141512994481076 1101 14-15 -0.4775679350334272 1101 16-17 -0.5051057028266399 1101 18-19 -0.5122154203674683 1101 20-21 -0.5173051935411763 1101 22-23 -0.5813229625577705 1101 24-25 -0.5508267634451585 1101 26-27 -0.5697895824441375 1101 28-29 -0.6221452465266211 1101 30-31 -0.532171890789563 1101 32-33 -0.45299928800154987 1101 34-35 -0.42794979201455163 1101 36-37 -0.4106023668386314 1101 38-39 -0.3553390493649786 1101 40-41 -0.33917352050978167 1101 42-43 -0.4374046687377344 1101 44-45 -0.4182851983578715 1101 46-47 -0.3500303020072124 1101 48-49 0.8437354594486024 1101 50-51 -0.05179898664388105 1101 52-53 -0.3945530476825567 1101 54-55 -0.36891347468602476 1101 56-57 -0.3989558188768214 1101 58-59 -0.4455819904786438 1101 60-61 -0.48338738105363177 1101 62-63 -0.4689973203004669 1101 64-65 -0.4319836147991438 1101 66-67 -0.37738923963609494 1101 68-69 -0.42334622806645683 1101 70-71 -0.4744692511310049 1101 72-73 -0.5443283835503294 1101 74-75 -0.5207194734862242 1101 76-77 -0.44696162997793465 1101 78-79 -0.45091329789975276 1101 80-81 -0.33419946988591676 1101 82-83 -0.32257800338882703 1101 84-85 -0.41835442646300436 1101 86-87 -0.44617850260685543 1101 88-89 -0.4115892492012314 1101 90-91 -0.49342502844822533 1101 92-93 -0.5316763031614151 1101 94-95 -0.5442746597625039 1101 96-97 -0.6681070193742826 1101 98-99 -0.6732681126884366 1101 100 -0.4923985322483091 1102 1 -0.20020973760840022 1102 2 -0.1618335359736136 1102 3 -0.2659887434137467 1102 4 -0.20389022798464396 1102 5 -0.11309902172062891 1102 6 -0.16283107998042112 1102 7 -0.17782884937465582 1102 8 -0.17558547151082848 1102 9 -0.29707657551458055 1102 10-11 -0.35282765288143736 1102 12-13 -0.4184499050909025 1102 14-15 -0.4967735367009283 1102 16-17 -0.48125910797470794 1102 18-19 -0.4922656075889549 1102 20-21 -0.5473876483219655 1102 22-23 -0.5043486638168488 1102 24-25 -0.5020647658727029 1102 26-27 -0.581561638193449 1102 28-29 -0.600559642196707 1102 30-31 -0.6896395613927737 1102 32-33 -0.5619690811313518 1102 34-35 -0.4962284798239551 1102 36-37 -0.4646492278839176 1102 38-39 -0.49954310009236025 1102 40-41 -0.5272080995502151 1102 42-43 -0.4877398799293502 1102 44-45 -0.4760002028850181 1102 46-47 -0.4569577971776795 1102 48-49 0.7286058724981714 1102 50-51 -0.15185891897245796 1102 52-53 -0.4702754675846066 1102 54-55 -0.5742213240920151 1102 56-57 -0.5800327204514772 1102 58-59 -0.7273710597408609 1102 60-61 -0.6168737751662832 1102 62-63 -0.4709701161303954 1102 64-65 -0.6073962629299317 1102 66-67 -0.5184852664036796 1102 68-69 -0.6070554252334119 1102 70-71 -0.5825932715872426 1102 72-73 -0.6355495381654137 1102 74-75 -0.6519332700411482 1102 76-77 -0.6398721246033574 1102 78-79 -0.4693433494598054 1102 80-81 -0.35482263082114684 1102 82-83 -0.4187007691645306 1102 84-85 -0.49962997244795915 1102 86-87 -0.49330440844594037 1102 88-89 -0.49890859869811877 1102 90-91 -0.5112736675793208 1102 92-93 -0.5744096664168872 1102 94-95 -0.5872085045418522 1102 96-97 -0.5935852472568968 1102 98-99 -0.6937172681485144 1102 100 -0.6051383906408745 1103 1 -0.29145392967877726 1103 2 -0.26900370100448256 1103 3 -0.2690780681115328 1103 4 -0.13329047673033045 1103 5 -0.20729365211960982 1103 6 -0.25201910874138633 1103 7 -0.2468819023550708 1103 8 -0.30422182506835327 1103 9 -0.4117889759104756 1103 10-11 -0.3179071245085012 1103 12-13 -0.45222846095487057 1103 14-15 -0.5245124706422217 1103 16-17 -0.46004170545216994 1103 18-19 -0.4861012096684192 1103 20-21 -0.527840510610794 1103 22-23 -0.5235036138358922 1103 24-25 -0.6220224750740755 1103 26-27 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1105 68-69 -0.6092685114616643 1105 70-71 -0.5804370249688162 1105 72-73 -0.5773906232912722 1105 74-75 -0.6312563249978069 1105 76-77 -0.47335149210786653 1105 78-79 -0.40797367553774677 1105 80-81 -0.37643731627538557 1105 82-83 -0.3539309399764221 1105 84-85 -0.39715153161389694 1105 86-87 -0.39208750898517764 1105 88-89 -0.3896838581622646 1105 90-91 -0.40489020976900036 1105 92-93 -0.3076487045850804 1105 94-95 -0.4463964237976228 1105 96-97 -0.47290215810443925 1105 98-99 -0.5160847370935109 1105 100 -0.5488455273234649 1106 1 -0.30918667723434723 1106 2 -0.2578106887712721 1106 3 -0.27567822860386215 1106 4 -0.1898877699136392 1106 5 -0.24827638861005852 1106 6 -0.2557623902899664 1106 7 -0.2730838340361359 1106 8 -0.2709057725638857 1106 9 -0.35979053849767695 1106 10-11 -0.3426520206022232 1106 12-13 -0.38616579581999844 1106 14-15 -0.534103869484909 1106 16-17 -0.4876794469363688 1106 18-19 -0.5715774427556326 1106 20-21 -0.5737079870517121 1106 22-23 -0.5572925606482784 1106 24-25 -0.47585937714873694 1106 26-27 -0.5762122143178559 1106 28-29 -0.5465611973959525 1106 30-31 -0.5928047032435941 1106 32-33 -0.5377775511161715 1106 34-35 -0.5632060672151411 1106 36-37 -0.6052716740861896 1106 38-39 -0.5989474244861057 1106 40-41 -0.6125738289520015 1106 42-43 -0.5473983626447776 1106 44-45 -0.5504680938525723 1106 46-47 -0.7448331017229606 1106 48-49 0.509340513860046 1106 50-51 -0.3933862177073948 1106 52-53 -0.6857707815897314 1106 54-55 -0.8805825741504236 1106 56-57 -0.9437222966587271 1106 58-59 -0.8631155538789059 1106 60-61 -0.8218311562207816 1106 62-63 -0.8654913863296585 1106 64-65 -0.8731998547155655 1106 66-67 -0.8800523450261721 1106 68-69 -0.8288792056389411 1106 70-71 -0.7573794411949066 1106 72-73 -0.7371096456507971 1106 74-75 -0.7502774386845701 1106 76-77 -0.819591772811858 1106 78-79 -0.6580029221377686 1106 80-81 -0.5836536092222957 1106 82-83 -0.5918065814527509 1106 84-85 -0.5447135281947588 1106 86-87 -0.5053433067523123 1106 88-89 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2208 48-49 2.2273058670041763 2208 50-51 1.3311313294721785 2208 52-53 1.044979878372672 2208 54-55 1.039788184675949 2208 56-57 1.0677894440726519 2208 58-59 1.028025994485951 2208 60-61 1.0100806026021019 2208 62-63 1.0406902578531927 2208 64-65 1.133065631394004 2208 66-67 1.0295838236871688 2208 68-69 1.036533516561903 2208 70-71 1.0373887239615414 2208 72-73 1.123261481227722 2208 74-75 1.0999949712665824 2208 76-77 1.0246137495024144 2208 78-79 0.9316548888635765 2208 80-81 0.9133851705891587 2208 82-83 0.9430043423232135 2208 84-85 0.9665847188116139 2208 86-87 1.043052057798203 2208 88-89 1.0047029905459546 2208 90-91 1.0345121542212539 2208 92-93 0.9974809340833026 2208 94-95 0.9880904502979391 2208 96-97 1.0182897954199746 2208 98-99 1.1232290026669993 2208 100 1.3514231115062607 2209 1 0.32773413976337906 2209 2 0.36163645738522376 2209 3 0.42512860845449296 2209 4 0.2475430857547707 2209 5 0.3616209017677221 2209 6 0.3776707114203006 2209 7 0.41847144967704963 2209 8 0.47358858392173175 2209 9 0.5930041458699407 2209 10-11 0.5956194295988126 2209 12-13 0.6334920838763836 2209 14-15 0.7744674373304292 2209 16-17 0.7890917342075809 2209 18-19 0.7492685496305569 2209 20-21 0.7567697480557314 2209 22-23 0.7785337287368392 2209 24-25 0.8092379513315393 2209 26-27 0.8679032897213403 2209 28-29 0.8533934305532327 2209 30-31 0.8451534040650728 2209 32-33 0.8599774097285007 2209 34-35 0.8810673616418185 2209 36-37 0.9576513388185148 2209 38-39 0.9265595225347099 2209 40-41 0.9791458135529965 2209 42-43 1.0114273785991656 2209 44-45 0.9653519620979338 2209 46-47 0.9862610415223685 2209 48-49 2.1777176149285893 2209 50-51 1.2721428053646733 2209 52-53 1.0288005820451147 2209 54-55 0.9858811298800845 2209 56-57 0.9696137439571899 2209 58-59 0.985641769988959 2209 60-61 0.970015995951897 2209 62-63 0.9447406378152863 2209 64-65 1.0119712555711047 2209 66-67 1.0346080915482148 2209 68-69 1.064747567046254 2209 70-71 1.029446704470267 2209 72-73 1.0898030829223941 2209 74-75 1.0474985788024682 2209 76-77 0.9806625780586486 2209 78-79 0.9444952980746066 2209 80-81 0.8752913694369653 2209 82-83 0.8572626648197215 2209 84-85 0.8966825773291056 2209 86-87 0.8794233823079303 2209 88-89 0.8888487102977116 2209 90-91 0.9483434113865812 2209 92-93 0.8935198722640756 2209 94-95 0.9750564182541304 2209 96-97 1.037533656550977 2209 98-99 1.0101808438453936 2209 100 1.2315366532402052 2210 1 0.5194364329137144 2210 2 0.44948851599837525 2210 3 0.43256971672774114 2210 4 0.3219767855582063 2210 5 0.3684080457097494 2210 6 0.4099086797821485 2210 7 0.43368639459620795 2210 8 0.4459768178962449 2210 9 0.5965294668760492 2210 10-11 0.6045842021317611 2210 12-13 0.6129899849018656 2210 14-15 0.7495026041175379 2210 16-17 0.7521829729427196 2210 18-19 0.7371177581250166 2210 20-21 0.7497912784039826 2210 22-23 0.7412960545807934 2210 24-25 0.7514930755691296 2210 26-27 0.7789934233459377 2210 28-29 0.7636526166696598 2210 30-31 0.7860264505845223 2210 32-33 0.7710072311639706 2210 34-35 0.8285192635903869 2210 36-37 0.8052569632979143 2210 38-39 0.8238660684299006 2210 40-41 0.8581815356535571 2210 42-43 0.7675545361849885 2210 44-45 0.772529020665111 2210 46-47 0.7983344201266149 2210 48-49 2.0860060359076655 2210 50-51 1.1974161409413213 2210 52-53 0.9308517940810717 2210 54-55 0.9310048985828345 2210 56-57 0.9282088744834383 2210 58-59 0.8871002492759601 2210 60-61 0.8557213635528527 2210 62-63 0.8288125656171772 2210 64-65 0.9093082333787947 2210 66-67 0.9520374407833714 2210 68-69 0.9304564559228794 2210 70-71 1.005568501994162 2210 72-73 0.9724128078951395 2210 74-75 0.8505652605471852 2210 76-77 0.8177913500988012 2210 78-79 0.7266844926310583 2210 80-81 0.7307719638062338 2210 82-83 0.7834471649548718 2210 84-85 0.8347568992307011 2210 86-87 0.7717651133780841 2210 88-89 0.8358315838425376 2210 90-91 0.861379762352037 2210 92-93 0.8340489738147276 2210 94-95 0.8072235473755391 2210 96-97 0.8657695066310538 2210 98-99 0.9512010392348174 2210 100 1.0022127013943383 2211 1 0.5281378682296776 2211 2 0.47701570434982443 2211 3 0.4779119616742591 2211 4 0.29960326349713284 2211 5 0.33715186247305695 2211 6 0.3679997972104232 2211 7 0.3884674118582865 2211 8 0.4533106509573557 2211 9 0.6203171946830395 2211 10-11 0.5760406819849138 2211 12-13 0.5581090519017735 2211 14-15 0.7036970524878399 2211 16-17 0.691342025916839 2211 18-19 0.6930553649060514 2211 20-21 0.6457898185786917 2211 22-23 0.6978263128589433 2211 24-25 0.7290845338022081 2211 26-27 0.7660462847645064 2211 28-29 0.7524838798300024 2211 30-31 0.794563834455964 2211 32-33 0.7761136230877028 2211 34-35 0.7453488933922827 2211 36-37 0.8101147287276049 2211 38-39 0.8101538185994102 2211 40-41 0.7985886408522163 2211 42-43 0.6787764063714548 2211 44-45 0.7388519604956301 2211 46-47 0.7917280398314048 2211 48-49 2.0425877727061774 2211 50-51 1.213933321617965 2211 52-53 0.9300318052086638 2211 54-55 0.9283277471742721 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0.9324941483532747 2310 98-99 0.9951055929747561 2310 100 1.1981819467699673 2311 1 0.28333661379274844 2311 2 0.3419955553628711 2311 3 0.33603998357093445 2311 4 0.23797245937922895 2311 5 0.21992547163326037 2311 6 0.2866219025990091 2311 7 0.3524367442335148 2311 8 0.16651870910776267 2311 9 0.40139222257747065 2311 10-11 0.3280241204564831 2311 12-13 0.4303711548349227 2311 14-15 0.34597476366403157 2311 16-17 0.3812548490498955 2311 18-19 0.563764316761528 2311 20-21 0.5759583677433042 2311 22-23 0.5890005690977276 2311 24-25 0.5497458213874751 2311 26-27 0.591539808562338 2311 28-29 0.6530315675712544 2311 30-31 0.46634256933067775 2311 32-33 0.5217058065084785 2311 34-35 0.6950890418737075 2311 36-37 0.7070883635254077 2311 38-39 0.7278703479905673 2311 40-41 0.6750098124544195 2311 42-43 0.7059976245546551 2311 44-45 0.7467395396201155 2311 46-47 0.852483915716526 2311 48-49 -6.133452445827277 2311 50-51 -1.0370142765722434 2311 52-53 0.33591197831662356 2311 54-55 0.7404541965111235 2311 56-57 0.7978305202961806 2311 58-59 0.8499482549447421 2311 60-61 0.6701243228830407 2311 62-63 0.5634895939797602 2311 64-65 0.5738573411043788 2311 66-67 0.7257556704065067 2311 68-69 0.7745037641817731 2311 70-71 0.7501766284975986 2311 72-73 0.28015307865427275 2311 74-75 0.6594926665180552 2311 76-77 0.6802612812901785 2311 78-79 0.6199364925564694 2311 80-81 0.6555910847714159 2311 82-83 0.6758355607256661 2311 84-85 0.812032805024419 2311 86-87 0.7329509432771992 2311 88-89 0.7276694173965303 2311 90-91 0.7272912901272228 2311 92-93 0.7929864027218052 2311 94-95 0.7687618116826975 2311 96-97 0.8457241003991172 2311 98-99 0.8277037803913281 2311 100 0.9984291595217734 2312 1 0.01816975519648878 2312 2 0.1801542235255411 2312 3 0.15609508554008045 2312 4 -0.07600183252996828 2312 5 0.14462620934529724 2312 6 0.09722445677213898 2312 7 0.2426985663878014 2312 8 0.32855158544533936 2312 9 0.26092411462123266 2312 10-11 0.19746348426071592 2312 12-13 0.232672461496513 2312 14-15 0.2400019717631494 2312 16-17 0.40432011096447695 2312 18-19 0.26217133195753917 2312 20-21 0.2842812188866546 2312 22-23 0.5353205117221833 2312 24-25 0.6214130857161067 2312 26-27 0.47207482077127594 2312 28-29 0.5700713570710079 2312 30-31 0.6432968546594324 2312 32-33 0.6453514782959431 2312 34-35 0.24924079779841435 2312 36-37 0.5526895175059323 2312 38-39 0.6624588715868143 2312 40-41 0.47009079257076536 2312 42-43 0.439148293597178 2312 44-45 0.524190038421402 2312 46-47 0.6329754433959778 2312 48-49 -4.559081793124953 2312 50-51 -0.8138618376492914 2312 52-53 0.45335465822058296 2312 54-55 0.6434927299965167 2312 56-57 0.7720370316077023 2312 58-59 0.8011617089934759 2312 60-61 0.838385260559221 2312 62-63 0.5193205874972833 2312 64-65 0.7366510811805753 2312 66-67 0.689997454556881 2312 68-69 0.6673929576523534 2312 70-71 0.7137034595151448 2312 72-73 0.740959908451849 2312 74-75 0.4342225171456917 2312 76-77 0.5706746754494816 2312 78-79 0.3258228468246003 2312 80-81 0.5268636611443043 2312 82-83 0.5059348727366384 2312 84-85 0.5399074050684227 2312 86-87 0.6236054432097369 2312 88-89 0.6780697199860093 2312 90-91 0.4601868745759319 2312 92-93 0.632161412010209 2312 94-95 0.7369380446377782 2312 96-97 0.7164910362601304 2312 98-99 0.7358995513475399 2312 100 0.3256855953245612 2313 1 0.05804806808324514 2313 2 0.22973917730899274 2313 3 0.28822669263142586 2313 4 0.022831470338388726 2313 5 0.05257511155711825 2313 6 0.1902361559978516 2313 7 0.10251488345441828 2313 8 0.23537717672835612 2313 9 0.41026746143148074 2313 10-11 0.2770652170673884 2313 12-13 0.026457745074402794 2313 14-15 0.04203079110902763 2313 16-17 0.011073743054055285 2313 18-19 0.32093089759776916 2313 20-21 0.32804435564532497 2313 22-23 0.41521821003875203 2313 24-25 0.24475458432263508 2313 26-27 0.4189183554701117 2313 28-29 0.4009486096507189 2313 30-31 0.4934441759297741 2313 32-33 0.31988291247139244 2313 34-35 0.2793071488601768 2313 36-37 -0.40326018953836495 2313 38-39 0.0523407872624162 2313 40-41 0.2995189783199521 2313 42-43 0.4321651050095596 2313 44-45 0.3614242591833303 2313 46-47 0.21356987480514533 2313 48-49 -5.419494681711221 2313 50-51 -1.5349983441291712 2313 52-53 -0.14634624516749284 2313 54-55 0.06644822481778334 2313 56-57 0.1411890752717042 2313 58-59 0.21286148725125287 2313 60-61 0.5210381310003669 2313 62-63 0.5155814474686053 2313 64-65 0.5868261421281247 2313 66-67 0.678880930527562 2313 68-69 0.684067277624834 2313 70-71 0.6269347037984687 2313 72-73 0.38247380436799716 2313 74-75 0.5692925871211045 2313 76-77 0.6225609892228263 2313 78-79 0.5503689863981762 2313 80-81 -0.21258752360495947 2313 82-83 0.08792684480666679 2313 84-85 0.4200967406067839 2313 86-87 0.4954224500172515 2313 88-89 0.4965929781667562 2313 90-91 0.5109737649886661 2313 92-93 0.5854217966559894 2313 94-95 0.5267972654390043 2313 96-97 0.5318668630653534 2313 98-99 0.669772855279664 2313 100 0.7205565312540827 2314 1 0.009452623288076722 2314 2 0.14379057339782975 2314 3 0.18869726129844366 2314 4 0.051594793351505075 2314 5 0.21795427497875153 2314 6 0.28321230680335674 2314 7 0.13052617188422033 2314 8 0.2532199888736102 2314 9 0.39863026228884024 2314 10-11 0.29841103790188583 2314 12-13 0.23753798864309061 2314 14-15 0.25899191999785387 2314 16-17 0.41240307937511034 2314 18-19 0.5176630961148021 2314 20-21 0.571844306821589 2314 22-23 0.28380216640218947 2314 24-25 0.3480442877358172 2314 26-27 0.4102618924570933 2314 28-29 0.5185259013839456 2314 30-31 0.4272251806913445 2314 32-33 0.4287045996441279 2314 34-35 0.333107150279325 2314 36-37 0.37294595806613984 2314 38-39 0.42911518186763686 2314 40-41 0.1964341027982428 2314 42-43 0.2403580091191202 2314 44-45 0.48289068056482876 2314 46-47 0.5926930360203215 2314 48-49 -6.468975514073598 2314 50-51 -1.5755652958331083 2314 52-53 -0.8252931133633581 2314 54-55 -0.47383388500073664 2314 56-57 -0.14178709139997636 2314 58-59 0.2917360916381355 2314 60-61 0.5264703121219014 2314 62-63 0.4347330360185353 2314 64-65 0.5873504199957651 2314 66-67 0.6383228594734547 2314 68-69 0.6978989338228416 2314 70-71 0.5146034631399488 2314 72-73 0.6943095929108622 2314 74-75 0.5898956286401074 2314 76-77 0.5891123301106092 2314 78-79 0.4148666648905319 2314 80-81 0.520264415336186 2314 82-83 0.47952250410368435 2314 84-85 0.5303080952249708 2314 86-87 0.5370417917817214 2314 88-89 0.4420943252269147 2314 90-91 0.610773229778566 2314 92-93 0.699369205403567 2314 94-95 0.6926489984284068 2314 96-97 0.7342897180942316 2314 98-99 0.7488993241512638 2314 100 -0.5895146192783969 2315 1 0.11243848281829116 2315 2 0.162899928311937 2315 3 0.2927251383719067 2315 4 0.12234612181324422 2315 5 0.1888341961303368 2315 6 0.25963962980688393 2315 7 0.2839390983496557 2315 8 0.22175215895795475 2315 9 0.38249954887223225 2315 10-11 0.19162761239542192 2315 12-13 0.3925501389981463 2315 14-15 0.19652405742918688 2315 16-17 0.29567960650460634 2315 18-19 0.3756820297020269 2315 20-21 0.2788541981042698 2315 22-23 0.3704141980034237 2315 24-25 0.3677664020694493 2315 26-27 0.4520294468844952 2315 28-29 0.4910139830370852 2315 30-31 0.49143263210711297 2315 32-33 0.6041070049676023 2315 34-35 0.5569095583525225 2315 36-37 0.4324802703594841 2315 38-39 0.5529300926046332 2315 40-41 0.5580940612773659 2315 42-43 0.5821265302473364 2315 44-45 0.620383247709718 2315 46-47 0.47976268230234353 2315 48-49 -4.832402902383134 2315 50-51 -0.9662565900263687 2315 52-53 0.11574103738353614 2315 54-55 0.12605619037615412 2315 56-57 0.44904235508003154 2315 58-59 0.5693902224151373 2315 60-61 0.35368117835457724 2315 62-63 0.13739234401543143 2315 64-65 0.3964011240976717 2315 66-67 -0.3223396356285875 2315 68-69 0.4186189196961152 2315 70-71 0.4336608095505312 2315 72-73 0.5326841993562184 2315 74-75 0.6302842380689171 2315 76-77 0.5784716180001226 2315 78-79 0.5125834464728598 2315 80-81 0.41077147783747137 2315 82-83 0.38673761522701255 2315 84-85 0.3858914153825346 2315 86-87 0.4115677217049374 2315 88-89 0.37305347717681414 2315 90-91 0.36338327899867195 2315 92-93 0.4273105752143138 2315 94-95 0.11199863219917816 2315 96-97 0.2771765187184201 2315 98-99 0.2363043296850904 2315 100 0.335692856197479 2316 1 0.25839904533216895 2316 2 0.26979753694443787 2316 3 0.20052004379370914 2316 4 -0.015178868361559239 2316 5 0.15439613331742663 2316 6 0.13020430096181457 2316 7 0.18836000978618017 2316 8 0.25563894278653976 2316 9 0.46101963926404466 2316 10-11 0.40328280565277197 2316 12-13 0.3196807115935414 2316 14-15 0.4416048376352606 2316 16-17 0.3743824775980187 2316 18-19 0.08188140857870252 2316 20-21 0.422489980189944 2316 22-23 0.5439227437888405 2316 24-25 0.5199126736208086 2316 26-27 0.595909790937263 2316 28-29 0.5916738731657532 2316 30-31 0.5276533273893094 2316 32-33 0.4936626070385799 2316 34-35 0.4936175620433758 2316 36-37 0.3713072593210853 2316 38-39 0.45196604855357947 2316 40-41 0.011439968014883561 2316 42-43 0.00514696665695169 2316 44-45 0.2720408225093749 2316 46-47 0.25157392531791345 2316 48-49 -5.171707427954278 2316 50-51 -1.1178191701230915 2316 52-53 0.06807126438830124 2316 54-55 0.3482480529771763 2316 56-57 0.24496137409818886 2316 58-59 0.1125581862925884 2316 60-61 0.21987972422542867 2316 62-63 0.12550935570313726 2316 64-65 0.0774602269290412 2316 66-67 0.1620432138493939 2316 68-69 -0.12328442386111504 2316 70-71 -0.4811756116001291 2316 72-73 -0.36480241915273837 2316 74-75 -0.13534951917181814 2316 76-77 -0.12073029834046167 2316 78-79 -0.0017705254697872874 2316 80-81 -0.12671554649483596 2316 82-83 -0.15092407333987268 2316 84-85 -0.01879135891133288 2316 86-87 0.07141705444591295 2316 88-89 -0.05346820019789078 2316 90-91 0.003732906495557131 2316 92-93 0.050210599563715874 2316 94-95 0.24853720464077966 2316 96-97 0.24074896032257698 2316 98-99 0.2291963336835181 2316 100 -0.5715922069400925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 8.0 11 13.0 12 34.0 13 164.0 14 662.0 15 2075.0 16 5271.0 17 11888.0 18 20453.0 19 27348.0 20 34870.0 21 46468.0 22 56255.0 23 52836.0 24 48312.0 25 48652.0 26 54809.0 27 67496.0 28 82175.0 29 101436.0 30 125461.0 31 155992.0 32 196774.0 33 257283.0 34 360342.0 35 496966.0 36 761562.0 37 1282084.0 38 1281501.0 39 181973.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.949590366382694 22.001644264217806 14.311245152502467 21.73752021689703 2 14.780334962256122 22.50466797209614 42.50021740764341 20.214779658004325 3 16.384461943933747 32.032217683031824 31.276834761743423 20.306485611291013 4 11.039911525704161 19.302691990176854 41.65969167680145 27.99770480731753 5 10.704375116838673 39.74075310324381 35.78091800784878 13.773953772068731 6 24.757484704096363 40.92223767236694 21.437767208575046 12.882510414961649 7 25.21133246418314 33.935842690641834 25.583929550580148 15.268895294594879 8 25.27463657640015 35.37844362162876 22.59128963715703 16.755630164814058 9 27.469839821573398 16.86676818429268 23.082674371451745 32.58071762268217 10-11 22.518157915017973 28.33390671681429 30.664641136000544 18.483294232167193 12-13 23.620634859194716 26.777043881438935 30.271734121273358 19.33058713809299 14-15 21.753394574270303 27.707679278842058 28.30507436064736 22.233851786240283 16-17 19.433887904955473 30.327163380863293 29.55706853820667 20.681880175974566 18-19 19.236638081117324 29.258767205224757 32.17450365254654 19.330091061111375 20-21 20.036523774040003 28.96224019423802 31.95179320066257 19.04944283105941 22-23 20.214267296643033 28.555439751501797 31.480592869311145 19.74970008254402 24-25 19.840909396638622 28.948874645092392 31.88363673603466 19.326579222234326 26-27 19.304564523787352 28.951101436398076 32.186010803513135 19.55832323630144 28-29 19.25958969854872 28.24945800563431 32.76875369936106 19.722198596455904 30-31 20.118046619796566 28.75383894092531 32.11660625211225 19.01150818716587 32-33 20.010564835952106 28.963916120479748 32.02181640360034 19.003702639967802 34-35 19.89036293839547 28.991044593701215 32.08240800153331 19.036184466370003 36-37 20.195414545611744 28.671425190918814 31.382179822632615 19.750980440836827 38-39 19.522471093254456 29.56925192595172 31.683708063506515 19.22456891728731 40-41 19.470735429423826 28.58489373057107 32.748826810813135 19.195544029191968 42-43 19.522090914306332 28.736184960422325 31.536257483982716 20.205466641288627 44-45 19.083353744834994 29.0916844149809 31.784370871227697 20.04059096895641 46-47 19.375892070067795 28.608849821143316 31.465609277563424 20.549648831225465 48-49 19.49681981654697 29.151583546421804 31.11559667041667 20.235999966614553 50-51 19.554613476854602 28.73298275343787 30.992796882610307 20.71960688709723 52-53 19.325961297244557 29.35585684511693 30.715852539602484 20.602329318036023 54-55 19.7448483790096 29.254589875315375 30.6551610644282 20.345400681246826 56-57 19.768072333338278 29.440980322159465 30.19152038066085 20.599426963841402 58-59 19.16822905008101 28.99904551472205 31.050403367023417 20.782322068173528 60-61 20.051622339511443 30.045845442309314 30.10478611072826 19.797746107450983 62-63 19.549372848253356 29.52552689964305 31.045048654449342 19.880051597654255 64-65 19.47376251822047 28.89092611741242 30.645386704592564 20.98992465977454 66-67 19.615118259367133 29.164905401274638 29.849730529650593 21.370245809707637 68-69 19.100388106516814 29.41512291486616 30.42084011756489 21.063648861052137 70-71 19.700966221319373 28.72307732374925 30.907336510664894 20.668619944266478 72-73 19.6010711577883 29.36138362973803 29.85523327492222 21.182311937551447 74-75 19.433313746236283 29.66315111546637 29.81578291825758 21.08775222003977 76-77 19.24165815288012 29.151332447378724 30.65608960698857 20.95091979275259 78-79 19.025331192260893 29.117239860089057 30.123017926143763 21.734411021506286 80-81 18.958661852812106 29.823786990146957 29.51572471530819 21.70182644173275 82-83 19.99191708835329 29.371167354897544 29.513451598575458 21.123463958173712 84-85 19.237075339721713 29.624531929088043 29.187085561648203 21.951307169542044 86-87 18.739108656908694 29.893250297105684 30.159720889936775 21.207920156048846 88-89 19.43846241933382 29.12599191044028 29.98093555969139 21.45461011053451 90-91 19.639705879161493 29.150923782667252 30.126317790612735 21.08305254755852 92-93 19.68887923281552 29.495582331966602 29.653116558922655 21.162421876295227 94-95 19.12094178423274 28.6168379473742 29.936853032169758 22.325367236223297 96-97 19.7696283930801 28.771567829142157 29.711742137108217 21.74706164066952 98-99 19.07562156467527 28.94208382003553 29.884780804549223 22.09751381073998 100 19.01490671366434 28.58253388086784 29.8393860205065 22.56317338496132 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3666.0 1 2583.5 2 4609.0 3 6509.0 4 3962.0 5 2757.0 6 3576.0 7 5677.5 8 8389.0 9 9803.5 10 8967.0 11 7752.0 12 7828.0 13 8522.0 14 9332.5 15 10155.5 16 10842.5 17 11477.5 18 12151.5 19 12971.5 20 14058.5 21 15320.0 22 16641.0 23 18512.0 24 20219.0 25 23906.5 26 33600.0 27 43780.0 28 47209.0 29 57558.5 30 83032.5 31 104898.5 32 134902.5 33 177406.5 34 222200.5 35 260459.5 36 301460.0 37 338431.0 38 331847.0 39 311226.5 40 280331.0 41 245148.5 42 217945.0 43 200355.5 44 202542.5 45 208274.5 46 193890.5 47 165807.5 48 151133.0 49 146220.5 50 145679.0 51 134472.0 52 116108.5 53 103470.0 54 93773.5 55 83777.5 56 73695.0 57 62696.0 58 52045.5 59 43038.0 60 33183.5 61 25113.0 62 19943.0 63 15271.5 64 10819.5 65 7506.0 66 5450.5 67 4255.0 68 3301.0 69 2467.0 70 1856.0 71 1422.5 72 1132.5 73 871.0 74 657.5 75 460.0 76 297.0 77 163.0 78 90.5 79 60.5 80 33.0 81 18.5 82 12.5 83 8.0 84 6.5 85 5.0 86 4.5 87 3.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030757550483954087 2 0.002082904095978832 3 5.2072602399470806E-5 4 0.022946660124033468 5 6.595862970599635E-4 6 6.943013653262774E-5 7 6.943013653262774E-5 8 0.003645082167962956 9 0.010605453355358887 10-11 0.0010935246503888869 12-13 6.943013653262774E-5 14-15 1.3886027306525547E-4 16-17 2.3432671079761863E-4 18-19 1.7357534133156934E-5 20-21 1.3886027306525547E-4 22-23 3.211143814634033E-4 24-25 7.81089035992062E-5 26-27 9.546643773236314E-5 28-29 9.893794455899452E-4 30-31 0.0027424903930387957 32-33 0.00985040062056656 34-35 0.018937069739274216 36-37 0.022182928622174563 38-39 0.02156673616044749 40-41 0.004990291063282619 42-43 0.005215939007013659 44-45 0.009477213636703686 46-47 0.012306491700408265 48-49 1.216494100955237 50-51 0.019292899189003933 52-53 0.01964872863873365 54-55 0.009945867058298924 56-57 0.008132004741384024 58-59 0.008513870492313476 60-61 0.005363478047145493 62-63 0.007984465701252191 64-65 0.020533962879524654 66-67 0.03182503883314324 68-69 0.03149524568461325 70-71 0.028414283375977904 72-73 0.02496013408347967 74-75 0.010275660206828905 76-77 0.016003646470770693 78-79 0.009069311584574498 80-81 0.003063604774502199 82-83 0.015387454009043622 84-85 0.02620119777400039 86-87 0.02520313956134387 88-89 0.035331260728040936 90-91 0.022729690947369006 92-93 0.01477994031438313 94-95 0.0072814855688593345 96-97 0.0040616629871587226 98-99 0.015717247157573604 100 0.035357297029240675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5761187.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.99284593370782 #Duplication Level Percentage of deduplicated Percentage of total 1 78.17967791310733 32.829871697520154 2 13.36584417390575 11.225396703375408 3 3.9057923279438946 4.920460064292179 4 1.5870530068714175 2.6657948962471667 5 0.8274632581313581 1.7373768557257012 6 0.4688441767479583 1.1812860768655853 7 0.31352104581829604 0.9215948681815861 8 0.21114460895314915 0.7093250426802061 9 0.15797325132411438 0.5970371764050396 >10 0.7581660967351143 5.710836507717004 >50 0.07547532880742053 2.2433780683526097 >100 0.10491791951033706 10.046913747362632 >500 0.024627576292066654 7.172290174322171 >1k 0.018425959015936848 13.77921081799324 >5k 7.75202159246534E-4 1.948944807613747 >10k+ 2.981546766332823E-4 2.3102824953457097 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30579 0.5307760362578059 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24135 0.41892408630374267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15549 0.2698922982364572 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11491 0.19945542472410635 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 10507 0.1823756111370799 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 9262 0.16076548114129952 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 6906 0.11987113072358178 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 6260 0.10865816367356242 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 6177 0.10721748834051038 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 5963 0.1035029760360148 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5824 0.10109027879150599 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7357534133156934E-5 0.0 5 0.0 1.7357534133156934E-5 0.0 3.471506826631387E-5 0.0 6 0.0 1.7357534133156934E-5 0.0 3.471506826631387E-5 0.0 7 0.0 1.7357534133156934E-5 0.0 3.471506826631387E-5 0.0 8 0.0 1.7357534133156934E-5 0.0 3.471506826631387E-5 0.0 9 0.0 1.7357534133156934E-5 0.0 3.471506826631387E-5 0.0 10-11 0.0 1.7357534133156934E-5 0.0 4.339383533289234E-5 0.0 12-13 0.0 1.7357534133156934E-5 0.0 6.075136946604927E-5 1.7357534133156934E-5 14-15 0.0 1.7357534133156934E-5 0.0 6.943013653262774E-5 3.471506826631387E-5 16-17 0.0 1.7357534133156934E-5 0.0 7.81089035992062E-5 3.471506826631387E-5 18-19 0.0 1.7357534133156934E-5 0.0 1.2150273893209854E-4 5.2072602399470806E-5 20-21 0.0 2.60363011997354E-5 0.0 2.5168424493077554E-4 5.2072602399470806E-5 22-23 0.0 3.471506826631387E-5 0.0 0.0011282397186552007 5.2072602399470806E-5 24-25 0.0 3.471506826631387E-5 0.0 0.0035062218948977006 5.2072602399470806E-5 26-27 0.0 3.471506826631387E-5 0.0 0.007151304062860657 5.2072602399470806E-5 28-29 0.0 3.471506826631387E-5 0.0 0.01415506908558948 5.2072602399470806E-5 30-31 0.0 3.471506826631387E-5 0.0 0.033777761423123394 5.2072602399470806E-5 32-33 0.0 3.471506826631387E-5 0.0 0.07767496524587728 5.2072602399470806E-5 34-35 0.0 3.471506826631387E-5 0.0 0.1418718052373582 5.2072602399470806E-5 36-37 0.0 3.471506826631387E-5 0.0 0.23588888886960274 5.2072602399470806E-5 38-39 0.0 3.471506826631387E-5 0.0 0.37832481396628853 5.2072602399470806E-5 40-41 0.0 3.471506826631387E-5 0.0 0.5494874580533491 5.2072602399470806E-5 42-43 0.0 3.471506826631387E-5 0.0 0.7175344247635079 5.2072602399470806E-5 44-45 0.0 3.471506826631387E-5 0.0 0.9107845310350107 6.075136946604927E-5 46-47 0.0 3.471506826631387E-5 0.0 1.1279099255066707 6.943013653262774E-5 48-49 0.0 3.471506826631387E-5 0.0 1.3595375397465834 6.943013653262774E-5 50-51 0.0 3.471506826631387E-5 0.0 1.614250674383595 6.943013653262774E-5 52-53 0.0 3.471506826631387E-5 0.0 1.8764275486978639 6.943013653262774E-5 54-55 0.0 3.471506826631387E-5 0.0 2.1644324338022702 6.943013653262774E-5 56-57 0.0 3.471506826631387E-5 0.0 2.4789856673633404 6.943013653262774E-5 58-59 0.0 3.471506826631387E-5 0.0 2.833426514362405 6.943013653262774E-5 60-61 0.0 3.471506826631387E-5 0.0 3.1938730681715417 6.943013653262774E-5 62-63 0.0 3.471506826631387E-5 0.0 3.5647167849264396 6.943013653262774E-5 64-65 0.0 3.471506826631387E-5 0.0 3.9389538996043694 6.943013653262774E-5 66-67 0.0 3.471506826631387E-5 0.0 4.3427161798428 6.943013653262774E-5 68-69 0.0 3.471506826631387E-5 0.0 4.7836930132627185 6.943013653262774E-5 70-71 0.0 3.471506826631387E-5 0.0 5.257171829346973 6.943013653262774E-5 72-73 0.0 3.471506826631387E-5 0.0 5.704293229850029 6.943013653262774E-5 74-75 0.0 3.471506826631387E-5 0.0 6.153245850204133 6.943013653262774E-5 76-77 0.0 3.471506826631387E-5 0.0 6.631341770367808 7.81089035992062E-5 78-79 0.0 3.471506826631387E-5 0.0 7.145037993038587 8.678767066578468E-5 80-81 0.0 3.471506826631387E-5 0.0 7.678443695717567 8.678767066578468E-5 82-83 0.0 3.471506826631387E-5 0.0 8.212326730585207 1.3886027306525547E-4 84-85 0.0 3.471506826631387E-5 0.0 8.734024776491372 1.3886027306525547E-4 86-87 0.0 3.471506826631387E-5 0.0 9.28161158455714 1.3886027306525547E-4 88 0.0 3.471506826631387E-5 0.0 9.698834632515833 1.3886027306525547E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 7540 0.0 69.685524 2 ACCTGGG 7665 0.0 67.02655 3 CCTGGGG 4295 0.0 53.418365 4 GTACCTG 10315 0.0 51.360588 1 TATCACG 385 0.0 47.522552 2 TAACGCA 785 0.0 46.61974 4 GTACCCG 365 0.0 42.425438 1 GGTATCA 16465 0.0 41.06837 1 GTACACG 610 0.0 39.232464 1 GTATCAA 28850 0.0 36.808147 1 TATAACG 1050 0.0 33.50949 2 CTGGGGG 3975 0.0 32.452072 5 GACATAT 1780 0.0 30.05316 1 ATCAACG 34665 0.0 29.993246 3 TCACGCA 600 0.0 29.715105 4 CAACGCA 35395 0.0 29.566816 5 TCAACGC 35775 0.0 29.15444 4 AACGCAG 37155 0.0 28.443762 6 CATGGGT 3920 0.0 28.246902 4 GTACATG 36925 0.0 28.136 1 >>END_MODULE