FastQCFastQC Report
Mon 6 Jun 2016
ERR1211331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211331_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9632
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC400.41528239202657813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.24916943521594684No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG230.2387873754152824RNA PCR Primer, Index 31 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.1972591362126246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.18687707641196014No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG170.17649501661129569TruSeq Adapter, Index 1 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.16611295681063123No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT160.16611295681063123No Hit
CGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAA150.15573089700996678No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC120.12458471760797342No Hit
GTACTTTGTGTCACAGCTGAAGAAGATGAAGAAGTCATCCGGGGAAATTG110.11420265780730898No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG100.10382059800664453RNA PCR Primer, Index 33 (96% over 29bp)
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA100.10382059800664453No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG100.10382059800664453No Hit
GTCCCGCTTCTGGTACTTTGTGTCACAGCTGAAGAAGATGAAGAAGTCAT100.10382059800664453No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10382059800664453No Hit
GTATAGGAAGACGCTGGGTTGGTCCGATACTATTTACACGTGGTGGCAGC100.10382059800664453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGA200.002114261370.363287
GTATCAA200.002114261370.363281
TCAACGC200.002114261370.363284
TATCAAC200.002114261370.363282
ATCAACG200.002114261370.363283
GGGGAAT200.002114261370.363289
CAACGCA200.002114261370.363285
TGGGGGG301.3159614E-462.545146
ATGGGGG250.005118910756.2906235
AACGCAG250.005118910756.2906236
CGCAGAG250.005118910756.2906238
TACATGG459.737445E-441.6967622
ACATGGG500.00163486837.527083
GTACATG550.002610338634.1155321
Produced by FastQC (version 0.11.5)