##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211331_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9632 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.065406976744185 31.0 31.0 33.0 28.0 34.0 2 31.15687292358804 31.0 31.0 34.0 28.0 34.0 3 31.21511627906977 31.0 31.0 34.0 28.0 34.0 4 35.12354651162791 35.0 35.0 37.0 32.0 37.0 5 34.88631644518272 35.0 35.0 37.0 32.0 37.0 6 34.82443936877076 35.0 35.0 37.0 32.0 37.0 7 34.673484219269106 35.0 35.0 37.0 30.0 37.0 8 34.655211794019934 35.0 35.0 37.0 31.0 37.0 9 36.11181478405316 37.0 35.0 39.0 31.0 39.0 10-11 35.944455980066444 37.0 35.0 39.0 30.0 39.0 12-13 35.8578696013289 37.0 35.0 39.0 30.0 39.0 14-15 36.732454318936874 38.0 35.5 40.0 30.5 41.0 16-17 36.691912375415285 38.0 35.0 40.0 31.0 41.0 18-19 36.7062396179402 38.0 35.0 40.0 30.5 41.0 20-21 36.69917981727575 38.0 35.0 40.0 30.5 41.0 22-23 36.52787583056478 38.0 35.0 40.0 30.0 41.0 24-25 36.327346345514954 38.0 34.5 40.0 30.0 41.0 26-27 36.05305232558139 38.0 34.0 40.0 29.0 41.0 28-29 35.84546303986711 38.0 34.0 40.0 27.5 41.0 30-31 35.87468853820598 38.0 34.0 40.0 28.5 41.0 32-33 35.744289867109636 38.0 34.0 40.0 28.0 41.0 34-35 35.49750830564784 38.0 34.0 40.0 27.0 41.0 36-37 35.296719269102994 38.0 34.0 40.0 26.0 41.0 38-39 35.098214285714285 38.0 33.0 40.0 25.5 41.0 40-41 34.948401162790695 38.0 33.0 40.0 25.0 41.0 42-43 34.84681270764119 38.0 33.0 40.0 25.0 41.0 44-45 34.678519518272424 38.0 33.0 40.0 24.5 41.0 46-47 34.414970930232556 37.5 33.0 40.0 24.0 41.0 48-49 33.05035299003322 37.0 31.0 40.0 16.5 41.0 50-51 33.463714700996675 36.5 31.0 39.0 22.0 41.0 52-53 33.49885797342193 36.0 31.0 39.0 22.0 40.0 54-55 33.256281146179404 36.0 31.0 39.0 21.5 40.0 56-57 33.00358181063123 36.0 31.0 39.0 21.0 40.0 58-59 32.648411544850504 35.0 30.0 39.0 20.0 40.0 60-61 32.21937292358804 35.0 30.0 38.5 18.0 40.0 62-63 31.889898255813954 35.0 29.0 38.0 18.0 40.0 64-65 31.594580564784053 34.0 29.0 38.0 18.0 40.0 66-67 31.2984323089701 34.0 29.0 37.5 17.5 40.0 68-69 30.88605689368771 34.0 28.0 37.0 17.0 39.0 70-71 30.56514742524917 34.0 28.0 36.0 15.5 39.0 72-73 30.236451411960132 33.5 28.0 36.0 15.0 39.0 74-75 29.848058554817275 33.0 27.0 35.5 13.0 38.0 76-77 28.938174833887043 31.5 26.0 34.5 13.5 36.0 78-79 29.427740863787378 33.0 27.0 35.0 14.5 37.0 80-81 29.28732350498339 33.0 26.0 35.0 13.5 36.0 82-83 28.966777408637874 33.0 26.0 35.0 11.5 36.0 84-85 28.810527408637874 32.5 26.0 35.0 11.5 35.5 86-87 28.460029069767444 32.0 25.5 34.0 10.5 35.0 88-89 28.210288621262457 32.0 25.0 34.0 10.0 35.0 90-91 27.957122093023255 32.0 25.0 34.0 10.0 35.0 92-93 27.76936254152824 31.5 25.0 34.0 10.0 35.0 94-95 27.51437915282392 31.0 24.5 34.0 10.0 35.0 96-97 27.202553986710964 31.0 24.0 34.0 9.0 35.0 98-99 26.96267649501661 31.0 23.0 34.0 9.0 35.0 100 24.363579734219268 27.0 18.0 32.0 7.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2448628628316278 1101 2 0.6973508551633536 1101 3 -0.1940587190587273 1101 4 0.67381605662856 1101 5 1.1091836982461913 1101 6 1.1937977531727597 1101 7 -0.259317765567765 1101 8 -0.19214451636327823 1101 9 -0.41082279988530246 1101 10-11 0.3401980976199681 1101 12-13 0.8967698534104827 1101 14-15 -1.4908795775983492 1101 16-17 1.7197924326830574 1101 18-19 0.10502923002923126 1101 20-21 -0.750559481028219 1101 22-23 1.569469766344774 1101 24-25 -0.2787172896547929 1101 26-27 0.5650085880554556 1101 28-29 -2.431276102369857 1101 30-31 -0.6401667776667779 1101 32-33 1.1898383734321385 1101 34-35 -0.38052294927295094 1101 36-37 2.2131510705729482 1101 38-39 -0.2867923309329683 1101 40-41 0.4685667052854612 1101 42-43 0.8248944342694315 1101 44-45 1.4174892526454954 1101 46-47 -1.3159282268657435 1101 48-49 -0.5234178870897566 1101 50-51 -1.6356320473508035 1101 52-53 -1.1853869510119566 1101 54-55 -0.8697753057127926 1101 56-57 0.6192649364524385 1101 58-59 0.7851426351426269 1101 60-61 1.6478658783346276 1101 62-63 -0.5613599219068028 1101 64-65 -0.933130555786807 1101 66-67 1.4714542835636593 1101 68-69 1.86813292321105 1101 70-71 0.7318620037370174 1101 72-73 0.8665869576025749 1101 74-75 1.4863426937645592 1101 76-77 -1.7225397143365875 1101 78-79 -1.2775109005577754 1101 80-81 -0.38210999070373575 1101 82-83 -0.2755561712593071 1101 84-85 0.2510728131821871 1101 86-87 0.5792345097032694 1101 88-89 1.1767412419756162 1101 90-91 1.8839498319966879 1101 92-93 1.3262579289922947 1101 94-95 1.7194160295722831 1101 96-97 1.2671942640692784 1101 98-99 -0.24548650134586936 1101 100 -1.4523495601620589 1102 1 0.36986286283162784 1102 2 0.5223508551633529 1102 3 0.6059412809412734 1102 4 1.1988160566285586 1102 5 0.6091836982461913 1102 6 -1.4062022468272417 1102 7 0.865682234432235 1102 8 1.1328554836367246 1102 9 0.6141772001146961 1102 10-11 1.090198097619968 1102 12-13 1.809269853410477 1102 14-15 1.8341204224016536 1102 16-17 1.2197924326830574 1102 18-19 -1.0324707699707716 1102 20-21 -0.6380594810282219 1102 22-23 -1.555530233655226 1102 24-25 1.0837827103452042 1102 26-27 0.3650085880554528 1102 28-29 -3.8937761023698556 1102 30-31 -1.7651667776667779 1102 32-33 0.0023383734321384964 1102 34-35 1.256977050727052 1102 36-37 -2.2993489294270546 1102 38-39 -0.8867923309329697 1102 40-41 -1.9939332947145374 1102 42-43 -1.0001055657305713 1102 44-45 -0.4575107473545046 1102 46-47 -0.9659282268657492 1102 48-49 0.0015821129102491227 1102 50-51 -1.3356320473508063 1102 52-53 0.4271130489880406 1102 54-55 -3.6697753057127933 1102 56-57 -1.8932350635475608 1102 58-59 -0.9523573648573738 1102 60-61 -0.014634121665373812 1102 62-63 -1.0488599219068035 1102 64-65 -3.4831305557868077 1102 66-67 -1.9285457164363393 1102 68-69 -0.8318670767889493 1102 70-71 -0.7556379962629833 1102 72-73 0.04158695760257203 1102 74-75 -2.6636573062354394 1102 76-77 -2.0225397143365846 1102 78-79 -1.052510900557774 1102 80-81 -1.744609990703733 1102 82-83 -2.2380561712593057 1102 84-85 -0.6489271868178115 1102 86-87 -0.3707654902967299 1102 88-89 -0.04825875802438517 1102 90-91 0.5839498319966907 1102 92-93 0.27625792899229396 1102 94-95 0.4319160295722817 1102 96-97 1.479694264069277 1102 98-99 0.15451349865412922 1102 100 -3.452349560162059 1103 1 0.15557714854591254 1103 2 0.39377942659192655 1103 3 -0.33691586191586964 1103 4 -0.9154696576571553 1103 5 -1.0336734446109546 1103 6 -0.6633451039700944 1103 7 0.9371108058608044 1103 8 0.7185697693510065 1103 9 -0.28582279988530246 1103 10-11 0.01876952619139871 1103 12-13 0.13784128198191326 1103 14-15 -1.6158795775983492 1103 16-17 -3.9587789958883732 1103 18-19 -5.153899341399338 1103 20-21 -2.04520233817108 1103 22-23 0.6587554806304823 1103 24-25 1.8552112817737765 1103 26-27 -1.2921342690874056 1103 28-29 -2.1009189595127182 1103 30-31 -1.3365953490953473 1103 32-33 2.038052659146423 1103 34-35 -1.193022949272951 1103 36-37 -1.8493489294270518 1103 38-39 0.9632076690670317 1103 40-41 1.3703524195711765 1103 42-43 -0.26439128001628376 1103 44-45 -0.3146536044973658 1103 46-47 -1.4944996554371741 1103 48-49 0.9230106843388128 1103 50-51 3.1143679526491965 1103 52-53 0.555684477559474 1103 54-55 0.5945104085729227 1103 56-57 2.5567649364524385 1103 58-59 1.2047854922854881 1103 60-61 1.049651592620343 1103 62-63 3.001140078093197 1103 64-65 0.9954408727846236 1103 66-67 -0.5642600021506254 1103 68-69 -0.7032956482175194 1103 70-71 -1.348495139120125 1103 72-73 0.5630155290311443 1103 74-75 1.4506284080502745 1103 76-77 2.5274602856634125 1103 78-79 1.1332033851565093 1103 80-81 3.0553900092962643 1103 82-83 1.8762295430264082 1103 84-85 -0.6774986153892435 1103 86-87 1.186377366846127 1103 88-89 -1.2696873294529532 1103 90-91 2.7053784034252573 1103 92-93 1.54054364327801 1103 94-95 0.7104874581437137 1103 96-97 3.865408549783563 1103 98-99 3.7545134986541306 1103 100 -4.880920988733486 1104 1 -1.7134704705017043 1104 2 -1.2609824781699785 1104 3 -0.36072538572539514 1104 4 -0.45118394337143997 1104 5 -0.47414963508714436 1104 6 0.360464419839424 1104 7 -0.13431776556776498 1104 8 0.8495221503033861 1104 9 0.46417720011469754 1104 10-11 0.5901980976199681 1104 12-13 1.0426031867438112 1104 14-15 -0.6575462442650206 1104 16-17 0.8031257660163931 1104 18-19 0.6258625633625599 1104 20-21 -0.5213928143615476 1104 22-23 -0.09719690032189732 1104 24-25 0.6587827103452071 1104 26-27 0.23167525472211992 1104 28-29 1.8395572309634787 1104 30-31 1.7348332223332221 1104 32-33 2.29400504009881 1104 34-35 -0.5680229492729509 1104 36-37 1.1506510705729482 1104 38-39 0.379874335733696 1104 40-41 1.8227333719521326 1104 42-43 -0.13343889906390416 1104 44-45 0.2091559193121597 1104 46-47 -0.1909282268657435 1104 48-49 -2.565084553756421 1104 50-51 -0.8022987140174678 1104 52-53 -2.5812202843452923 1104 54-55 -1.7447753057127926 1104 56-57 -1.6932350635475615 1104 58-59 -0.027357364857373057 1104 60-61 -2.6646341216653724 1104 62-63 -1.9988599219068028 1104 64-65 -1.7247972224534713 1104 66-67 -2.3618790497696764 1104 68-69 -3.0485337434556143 1104 70-71 -4.497304662929647 1104 72-73 -1.3834130423974251 1104 74-75 -0.013657306235440814 1104 76-77 -0.014206381003251778 1104 78-79 -1.6941775672244397 1104 80-81 -0.8196099907037357 1104 82-83 -0.004722837925974943 1104 84-85 -0.5405938534844772 1104 86-87 0.7875678430366051 1104 88-89 -1.4065920913577195 1104 90-91 0.46728316533002 1104 92-93 -0.42374207100770533 1104 94-95 -0.38475063709438473 1104 96-97 -3.6703057359307216 1104 98-99 -0.6621531680125337 1104 100 -0.6190162268287267 1105 1 0.5062264991952645 1105 2 0.36780540061790035 1105 3 0.8059412809412727 1105 4 -0.7466384888259867 1105 5 0.6546382437007381 1105 6 0.3756159349909396 1105 7 0.9111367798867818 1105 8 0.6146736654549017 1105 9 -0.9221864362489356 1105 10-11 0.1811071885290616 1105 12-13 -1.7452756011349706 1105 14-15 -1.0704250321438025 1105 16-17 -1.4392984764078491 1105 18-19 -1.1733798608798622 1105 20-21 -0.869877662846406 1105 22-23 0.1717424936175007 1105 24-25 0.6474190739815668 1105 26-27 -0.07135504830818462 1105 28-29 -1.8346851932789505 1105 30-31 -1.4015304140304181 1105 32-33 -1.838570717476955 1105 34-35 -0.6475684038184042 1105 36-37 -0.21298529306341152 1105 38-39 -0.21861051275114818 1105 40-41 -3.0257514765327187 1105 42-43 -2.322832838457842 1105 44-45 -1.048419838263598 1105 46-47 0.8886172276797097 1105 48-49 -0.14841788708975656 1105 50-51 1.3416406799219232 1105 52-53 1.2634766853516837 1105 54-55 -1.9379571238946127 1105 56-57 1.1022194819069853 1105 58-59 0.2112789987789867 1105 60-61 0.3353658783346276 1105 62-63 1.1375037144568374 1105 64-65 0.8850512623950131 1105 66-67 -0.09672753461816086 1105 68-69 -2.0864125313344033 1105 70-71 -0.11472890535388913 1105 72-73 -1.3265948605792452 1105 74-75 -0.9000209425990775 1105 76-77 0.34564210384523264 1105 78-79 -0.7888745369214121 1105 80-81 2.1917536456599045 1105 82-83 -1.338056171259307 1105 84-85 -2.203472641363266 1105 86-87 -0.12531094484218386 1105 88-89 0.9381048783392529 1105 90-91 1.2248589229057814 1105 92-93 1.5080761108104745 1105 94-95 2.6455523932086464 1105 96-97 0.6251488095238251 1105 98-99 1.2999680441086774 1105 100 -1.4523495601620589 1106 1 -1.7665007735320088 1106 2 0.004169036981537033 1106 3 -1.5576950826950906 1106 4 -2.837547579735073 1106 5 -0.2544526653901755 1106 6 -2.9880204286454237 1106 7 -0.3615904928404916 1106 8 -0.5671445163632782 1106 9 -0.10400461806712258 1106 10-11 -1.0916200841982118 1106 12-13 -0.1543665102258842 1106 14-15 -3.115879577598349 1106 16-17 -0.5302075673169426 1106 18-19 -1.1279253154253155 1106 20-21 -0.14260493557367226 1106 22-23 -1.873712051837039 1106 24-25 -3.352580926018433 1106 26-27 -3.844082321035451 1106 28-29 -3.425594284188037 1106 30-31 -0.9924395049395045 1106 32-33 -1.0203888992951349 1106 34-35 -0.7384774947274977 1106 36-37 -1.5311671112452316 1106 38-39 -2.3549741491147884 1106 40-41 -2.343933294714539 1106 42-43 -0.9137419293669282 1106 44-45 0.9061256162818552 1106 46-47 -1.656837317774837 1106 48-49 -1.42114515981703 1106 50-51 -2.5219956837144366 1106 52-53 -0.3274324055574098 1106 54-55 -0.5288662148036991 1106 56-57 -3.170507790820288 1106 58-59 -0.6523573648573731 1106 60-61 0.4262749692437211 1106 62-63 -1.2715871946340762 1106 64-65 -3.0694941921504437 1106 66-67 -1.5512729891636141 1106 68-69 -1.6773216222434968 1106 70-71 -1.2965470871720726 1106 72-73 -0.19023122421560856 1106 74-75 -1.9909300335081674 1106 76-77 -3.108903350700224 1106 78-79 -1.6525109005577754 1106 80-81 -0.8537008997946423 1106 82-83 -0.019874353077486973 1106 84-85 -0.3852908231814496 1106 86-87 -1.1253109448421839 1106 88-89 -0.652804212569837 1106 90-91 -2.911504713457859 1106 92-93 0.5535306562650213 1106 94-95 -0.6726294249731701 1106 96-97 -0.193033008657995 1106 98-99 -1.9273046831640492 1106 100 2.093104985292488 1107 1 0.5621705551393212 1107 2 1.2069662397787404 1107 3 1.2674797424797326 1107 4 1.1834314412439468 1107 5 0.18610677516927154 1107 6 -0.4985099391349337 1107 7 1.365682234432235 1107 8 1.2790093297905685 1107 9 0.944946430883931 1107 10-11 0.6286596360815011 1107 12-13 1.4785006226412563 1107 14-15 0.999505037786264 1107 16-17 0.8928693557599772 1107 18-19 0.41752923002923126 1107 20-21 0.5427097497410074 1107 22-23 1.5213928432678543 1107 24-25 2.245321171883667 1107 26-27 2.257316280363149 1107 28-29 2.21776235916861 1107 30-31 -0.3420898545898581 1107 32-33 0.0215691426629121 1107 34-35 2.6146693584193557 1107 36-37 1.8044972244191086 1107 38-39 2.9632076690670317 1107 40-41 3.425297474516235 1107 42-43 3.757586741961738 1107 44-45 3.388643098799342 1107 46-47 4.04945638851887 1107 48-49 4.428505189833324 1107 50-51 1.9220602603415031 1107 52-53 3.319420741295737 1107 54-55 3.303301617364127 1107 56-57 3.364457244144745 1107 58-59 3.7707195582195467 1107 60-61 5.296904339873095 1107 62-63 2.3088323857855038 1107 64-65 3.528407905751653 1107 66-67 3.7214542835636593 1107 68-69 3.2527483078264368 1107 70-71 3.8712850806600976 1107 72-73 3.7608177268333485 1107 74-75 4.274804232226099 1107 76-77 2.1428449010480257 1107 78-79 2.539796791749918 1107 80-81 3.324620778527038 1107 82-83 3.0080976748945396 1107 84-85 4.558765120874494 1107 86-87 4.944619125087883 1107 88-89 2.686356626591003 1107 90-91 3.8262575243043813 1107 92-93 4.441642544376908 1107 94-95 5.16653141418767 1107 96-97 2.656617340992355 1107 98-99 0.8698981140387438 1107 100 2.778419670607171 1108 1 0.7698628628316264 1108 2 0.12235085516335431 1108 3 0.3059412809412727 1108 4 0.5988160566285572 1108 5 -0.39081630175380866 1108 6 0.49379775317275687 1108 7 -0.3343177655677678 1108 8 0.7328554836367189 1108 9 0.6141772001146961 1108 10-11 0.29019809761997095 1108 12-13 -0.5407301465895173 1108 14-15 -0.8658795775983492 1108 16-17 -2.130207567316944 1108 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90-91 2.633949831996688 2311 92-93 2.7548293575637253 2311 94-95 2.781916029572283 2311 96-97 3.0082656926407054 2311 98-99 4.254513498654131 2311 100 2.119079011266514 2312 1 -2.4378294448606788 2312 2 -1.9468799140674165 2312 3 0.11363358863357931 2312 4 -1.4319531741406735 2312 5 -2.506200917138422 2312 6 -0.9600484006733936 2312 7 -2.7112408424908416 2312 8 -1.951759900978665 2312 9 -2.131976646039149 2312 10-11 -2.6405711331492654 2312 12-13 -2.3676532235125975 2312 14-15 -3.038956500675269 2312 16-17 -1.1455921827015558 2312 18-19 -0.9670861545861555 2312 20-21 -2.611136404105139 2312 22-23 -2.132453310578306 2312 24-25 -1.2931403665778731 2312 26-27 -1.3196067965599312 2312 28-29 -1.3206991792929372 2312 30-31 -0.14978216228216468 2312 32-33 -1.9015077804140148 2312 34-35 -1.654561410811418 2312 36-37 -2.041656621734745 2312 38-39 -0.8060231001637419 2312 40-41 -2.420856371637619 2312 42-43 -0.8577978734228751 2312 44-45 -1.8421261319698914 2312 46-47 -2.258235919173437 2312 48-49 -8.879187117858987 2312 50-51 -4.27024743196619 2312 52-53 -2.2190407971658033 2312 54-55 -0.9659291518666393 2312 56-57 -0.21246583277833508 2312 58-59 -0.7292804417804497 2312 60-61 -1.3184802755115257 2312 62-63 -1.114244537291416 2312 64-65 -1.1638997865560405 2312 66-67 -1.5862380241286473 2312 68-69 -0.24725169217356324 2312 70-71 -0.897945688570676 2312 72-73 -0.7391822731666586 2312 74-75 -2.917503460081594 2312 76-77 -2.780232022028894 2312 78-79 -2.767895515942392 2312 80-81 -2.7523022983960423 2312 82-83 -2.5303638635670005 2312 84-85 -2.0950810329716596 2312 86-87 -1.9399962595275007 2312 88-89 -1.1213356811013035 2312 90-91 -0.5198963218494654 2312 92-93 -0.8660497633153987 2312 94-95 -0.025776278120023477 2312 96-97 0.5412327256077383 2312 98-99 -1.360871116730486 2312 100 -0.8369649447774421 2313 1 0.2865295294982957 2313 2 0.40568418849668575 2313 3 0.47260794760794056 2313 4 0.6321493899618957 2313 5 0.44251703157952704 2313 6 -0.22286891349390459 2313 7 0.11568223443223502 2313 8 0.6828554836367218 2313 9 -0.7024894665519668 2313 10-11 -0.4514685690467033 2313 12-13 -1.124063479922846 2313 14-15 -2.1575462442650206 2313 16-17 -0.40520756731694263 2313 18-19 1.7091958966959027 2313 20-21 -0.6047261476948904 2313 22-23 0.9028030996781027 2313 24-25 0.20044937701187848 2313 26-27 -0.018324745277872978 2313 28-29 2.506223897630143 2313 30-31 2.5264998889998935 2313 32-33 1.6273383734321385 2313 34-35 -0.15135628260627954 2313 36-37 -0.7243489294270518 2313 38-39 0.6715410024003674 2313 40-41 0.11440003861878978 2313 42-43 1.6582277676027601 2313 44-45 0.7508225859788311 2313 46-47 1.7674051064675922 2313 48-49 -2.6067512204230923 2313 50-51 -0.5939653806841321 2313 52-53 -3.3312202843452887 2313 54-55 -3.411441972379457 2313 56-57 -3.276568396880897 2313 58-59 -1.8606906981907052 2313 60-61 1.0436992116679633 2313 62-63 -2.457193255240135 2313 64-65 -0.8914638891201427 2313 66-67 -0.27854571643634074 2313 68-69 -0.09020041012228219 2313 70-71 -0.7889713295963183 2313 72-73 -0.6334130423974251 2313 74-75 -2.013657306235441 2313 76-77 -1.9725397143365875 2313 78-79 -0.19417756722443968 2313 80-81 1.5970566759629286 2313 82-83 0.8286104954073608 2313 84-85 1.501072813182187 2313 86-87 1.037567843036605 2313 88-89 3.1350745753089484 2313 90-91 -0.78271683466998 2313 92-93 0.617924595658959 2313 94-95 1.1152493629056153 2313 96-97 1.4963609307359462 2313 98-99 -0.6204865013458694 2313 100 1.964317106504609 2314 1 -1.2551371371683722 2314 2 -1.5526491448366464 2314 3 -1.5690587190587273 2314 4 -1.07618394337144 2314 5 -1.0158163017538087 2314 6 -0.3062022468272403 2314 7 -0.759317765567765 2314 8 0.30785548363672177 2314 9 -1.1608227998853025 2314 10-11 -3.347301902380032 2314 12-13 -0.8532301465895173 2314 14-15 -2.490879577598349 2314 16-17 -7.342707567316943 2314 18-19 -3.1449707699707687 2314 20-21 -2.625559481028219 2314 22-23 -1.180530233655226 2314 24-25 -1.153717289654793 2314 26-27 -6.497491411944544 2314 28-29 -5.243776102369857 2314 30-31 -2.077666777666778 2314 32-33 -2.8726616265678615 2314 34-35 -3.193022949272951 2314 36-37 -5.349348929427052 2314 38-39 -7.286792330932968 2314 40-41 -7.093933294714539 2314 42-43 -3.3626055657305685 2314 44-45 -3.3950107473545046 2314 46-47 -2.1284282268657435 2314 48-49 -2.3984178870897566 2314 50-51 -1.5106320473508035 2314 52-53 -1.8728869510119566 2314 54-55 -2.9322753057127926 2314 56-57 -0.6932350635475615 2314 58-59 -3.464857364857373 2314 60-61 -2.5396341216653724 2314 62-63 -1.3738599219068028 2314 64-65 -1.933130555786807 2314 66-67 -3.2785457164363407 2314 68-69 -3.19436707678895 2314 70-71 -1.6431379962629826 2314 72-73 0.9915869576025749 2314 74-75 -1.7011573062354408 2314 76-77 -3.4725397143365875 2314 78-79 -5.777510900557775 2314 80-81 -2.9446099907037357 2314 82-83 0.3494438287406929 2314 84-85 -0.1239271868178129 2314 86-87 -3.7332654902967306 2314 88-89 0.11424124197561625 2314 90-91 -0.05355016800331214 2314 92-93 -2.4237420710077053 2314 94-95 -0.9680839704277169 2314 96-97 -0.9203057359307216 2314 98-99 -1.6204865013458694 2314 100 -5.577349560162059 2315 1 -1.221046228077462 2315 2 -1.3594673266548298 2315 3 -1.1031496281496374 2315 4 -1.7466384888259867 2315 5 -1.5271799381174418 2315 6 0.4665250259000331 2315 7 -1.8161359473859449 2315 8 -2.294417243636005 2315 9 -0.6494591635216693 2315 10-11 0.8174708248926947 2315 12-13 0.20926985341048265 2315 14-15 0.15684769512891705 2315 16-17 -2.2120257491351225 2315 18-19 -1.4461071336071285 2315 20-21 -1.0971503901191255 2315 22-23 -1.6464393245643194 2315 24-25 -2.4889445623820663 2315 26-27 -1.6168095937627243 2315 28-29 -0.56195792055167 2315 30-31 -3.219712232212231 2315 32-33 -2.7022070811133148 2315 34-35 -0.23847749472749769 2315 36-37 -3.3493489294270518 2315 38-39 -3.2186105127511517 2315 40-41 -2.162115112896352 2315 42-43 -2.0046510202760217 2315 44-45 0.5424892526454954 2315 46-47 -4.020473681411197 2315 48-49 -8.51205425072612 2315 50-51 -5.067450229168983 2315 52-53 -1.6910687691937767 2315 54-55 -4.483411669349156 2315 56-57 -0.6705077908202881 2315 58-59 0.9385517260517204 2315 60-61 -3.6646341216653724 2315 62-63 -2.271587194634076 2315 64-65 -3.433130555786807 2315 66-67 -2.733091170981794 2315 68-69 -1.6773216222434968 2315 70-71 -0.25109254171752937 2315 72-73 -0.7356857696701518 2315 74-75 -2.172748215326351 2315 76-77 -3.1543578961547674 2315 78-79 -3.5616018096486854 2315 80-81 -3.4446099907037357 2315 82-83 -2.6107834439865805 2315 84-85 -3.9761999140905395 2315 86-87 -2.2162200357512774 2315 88-89 -2.1073496671152903 2315 90-91 -3.7296865316396755 2315 92-93 -1.0373784346440686 2315 94-95 -2.1271748795186234 2315 96-97 -0.05666937229435831 2315 98-99 -1.2000319558913226 2315 100 0.5476504398379411 2316 1 -0.41585142288265686 2316 2 1.1080651408776383 2316 3 0.520226995226988 2316 4 0.6559589137714141 2316 5 0.8234694125319066 2316 6 1.3366548960299056 2316 7 -0.20574633699633438 2316 8 0.8614269122081524 2316 9 2.142748628686128 2316 10-11 0.8044838119056834 2316 12-13 1.5664127105533439 2316 14-15 1.8841204224016508 2316 16-17 1.8269352898259186 2316 18-19 -0.36818505568505344 2316 20-21 -0.688059481028219 2316 22-23 0.3730411949162047 2316 24-25 2.2123541389166377 2316 26-27 2.5650085880554556 2316 28-29 3.6847953262015736 2316 30-31 2.520547508047507 2316 32-33 1.680909802003569 2316 34-35 -0.9787372349872427 2316 36-37 3.079222499144379 2316 38-39 1.6060648119241705 2316 40-41 1.798923848142607 2316 42-43 2.4498944342694315 2316 44-45 2.185346395502634 2316 46-47 1.7197860588485483 2316 48-49 -2.291275029946899 2316 50-51 1.685796524077766 2316 52-53 1.055684477559474 2316 54-55 2.308796122858638 2316 56-57 0.4853363650238691 2316 58-59 0.41907120657119634 2316 60-61 0.1925087354774817 2316 62-63 3.143997220950336 2316 64-65 3.3525837299274848 2316 66-67 2.4357399978493746 2316 68-69 2.5109900660681888 2316 70-71 3.222933432308448 2316 72-73 4.134444100459714 2316 74-75 5.66491412233599 2316 76-77 5.884603142806274 2316 78-79 1.9903462422993705 2316 80-81 3.0553900092962643 2316 82-83 1.0905152573121235 2316 84-85 -1.0346414725320976 2316 86-87 -1.5993369188681612 2316 88-89 -1.6982587580243838 2316 90-91 -4.4374787394318815 2316 92-93 -2.030884928150563 2316 94-95 2.1390588867151408 2316 96-97 1.365408549783563 2316 98-99 2.6116563557969883 2316 100 3.2619361541236565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 3.0 15 9.0 16 24.0 17 55.0 18 78.0 19 86.0 20 104.0 21 127.0 22 152.0 23 188.0 24 195.0 25 201.0 26 242.0 27 272.0 28 334.0 29 409.0 30 415.0 31 496.0 32 597.0 33 680.0 34 838.0 35 1024.0 36 1237.0 37 1258.0 38 567.0 39 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.30024916943522 17.784468438538205 13.392857142857142 24.522425249169437 2 15.988372093023257 20.369601328903656 37.821843853820596 25.820182724252494 3 18.18936877076412 26.889534883720927 30.471345514950166 24.449750830564785 4 11.887458471760798 16.175249169435215 38.3201827242525 33.6171096345515 5 13.08139534883721 35.143272425249165 33.64825581395349 18.127076411960132 6 29.671926910299 33.94933554817276 20.24501661129568 16.13372093023256 7 25.228405315614616 31.322674418604652 24.23172757475083 19.2171926910299 8 26.868770764119603 32.589285714285715 21.117109634551497 19.42483388704319 9 25.86171096345515 16.933139534883722 22.684800664451828 34.520348837209305 10-11 24.942898671096344 25.015573089701 27.538413621262457 22.5031146179402 12-13 24.78716777408638 23.499792358803987 28.846553156146182 22.866486710963454 14-15 23.707433554817275 24.802740863787378 26.344476744186046 25.1453488372093 16-17 22.544642857142858 27.12313122923588 26.98297342192691 23.349252491694354 18-19 21.59468438538206 26.033014950166113 29.78093853820598 22.591362126245848 20-21 22.87686877076412 25.477574750830566 29.308554817275745 22.33700166112957 22-23 23.115656146179404 25.342607973421927 28.877699335548172 22.6640365448505 24-25 22.34738372093023 25.60735049833887 29.915905315614616 22.129360465116278 26-27 22.124169435215947 26.266611295681063 28.62333887043189 22.985880398671096 28-29 21.527200996677742 24.802740863787378 29.74979235880399 23.920265780730897 30-31 22.170888704318937 25.39451827242525 29.936669435215947 22.497923588039868 32-33 22.685978300368582 26.143383688937337 28.5884856979702 22.582152312723874 34-35 22.41433021806854 26.49532710280374 28.520249221183803 22.570093457943923 36-37 23.324160132924867 25.037644737525312 28.308842619035257 23.329352510514564 38-39 23.185169799563816 25.454356631010487 28.564752310728007 22.795721258697686 40-41 22.660021803457404 24.861132741525203 29.14914603125162 23.329699423765767 42-43 22.608108809635052 25.094741213725797 29.247780719514093 23.049369257125058 44-45 21.72490783529778 25.499766343008464 28.895581286671167 23.87974453502259 46-47 22.746625129802702 25.986500519210797 27.77258566978193 23.49428868120457 48-49 22.46300766082485 25.060342113548117 29.767026970301185 22.709623255325848 50-51 22.924347058518098 26.10208214341347 27.078249130276756 23.895321667791684 52-53 22.183573876025335 26.985775101235593 27.878724950680095 22.951926072058974 54-55 21.7296511627907 27.361918604651166 26.863579734219268 24.04485049833887 56-57 22.10859634551495 26.95701827242525 27.154277408637874 23.780107973421927 58-59 22.544642857142858 26.7234219269103 28.015988372093027 22.71594684385382 60-61 22.991071428571427 26.640365448504983 26.936254152823917 23.43230897009967 62-63 22.561387115194933 26.210870580906402 27.52426932461195 23.703472979286715 64-65 22.297788391651956 26.56006645208182 27.167480012459766 23.974665143806458 66-67 22.437960751739176 25.7449901360191 27.608763368289896 24.208285743951823 68-69 22.432769182847057 26.077250545114733 27.478974145986918 24.011006126051292 70-71 22.354895649465266 25.879970927214202 27.7177863150244 24.047347108296126 72-73 22.583324680718516 26.482192918700033 26.570449589866058 24.364032810715397 74-75 22.837711556432357 26.606790572110896 26.918284705638047 23.63721316581871 76-77 22.53140899179732 26.508150763160625 26.840411172256257 24.120029072785794 78-79 22.69116959975082 26.64174842963194 25.925349114883456 24.74173285573379 80-81 21.994393687707642 26.51058970099668 26.640365448504983 24.8546511627907 82-83 22.230297996054407 26.523725469836982 27.250545114733672 23.995431419374935 84-85 22.811753711971757 26.549683314297578 27.037690790156788 23.600872183573877 86-87 21.591735022323746 26.087633682898968 28.18502751531513 24.13560377946215 88-89 22.609282525179108 26.71062194995328 26.87156058560897 23.808534939258642 90-91 22.624857231855465 25.77613954937182 26.705430381061156 24.893572837711556 92-93 22.121275049319905 26.622365278787253 26.933859412314405 24.322500259578444 94-95 22.240681133838645 25.942269753919632 27.297269234762744 24.519779877478975 96-97 21.92285729117998 26.138192389555105 27.238747858589008 24.700202460675907 98-99 21.71746015264005 26.05783708011007 26.945641451638025 25.279061315611855 100 22.25799750727046 25.778977980889074 26.786456169505605 25.176568342334853 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 1.0 2 4.0 3 5.0 4 3.0 5 3.5 6 2.0 7 4.0 8 6.5 9 7.0 10 6.0 11 7.0 12 8.0 13 8.0 14 11.0 15 11.0 16 10.5 17 10.0 18 13.5 19 17.5 20 14.5 21 13.0 22 19.0 23 27.5 24 31.0 25 34.5 26 31.0 27 29.5 28 35.0 29 46.5 30 61.0 31 81.0 32 106.5 33 127.5 34 148.0 35 166.5 36 202.5 37 229.0 38 258.5 39 287.0 40 273.0 41 270.0 42 289.5 43 324.5 44 344.5 45 369.0 46 405.5 47 403.0 48 399.0 49 412.0 50 411.0 51 425.0 52 437.0 53 369.0 54 324.5 55 299.5 56 272.5 57 288.0 58 267.5 59 224.0 60 188.0 61 142.5 62 101.0 63 73.0 64 57.0 65 44.5 66 30.5 67 25.0 68 18.5 69 14.0 70 12.0 71 7.0 72 7.0 73 7.5 74 3.0 75 1.0 76 2.0 77 1.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.005191029900332226 34-35 0.020764119601328904 36-37 0.025955149501661133 38-39 0.031146179401993355 40-41 0.005191029900332226 42-43 0.005191029900332226 44-45 0.025955149501661133 46-47 0.020764119601328904 48-49 1.0693521594684385 50-51 0.025955149501661133 52-53 0.010382059800664452 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.005191029900332226 64-65 0.010382059800664452 66-67 0.010382059800664452 68-69 0.010382059800664452 70-71 0.010382059800664452 72-73 0.010382059800664452 74-75 0.010382059800664452 76-77 0.010382059800664452 78-79 0.005191029900332226 80-81 0.0 82-83 0.010382059800664452 84-85 0.010382059800664452 86-87 0.010382059800664452 88-89 0.010382059800664452 90-91 0.010382059800664452 92-93 0.010382059800664452 94-95 0.010382059800664452 96-97 0.005191029900332226 98-99 0.015573089700996677 100 0.04152823920265781 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9632.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.02616279069767 #Duplication Level Percentage of deduplicated Percentage of total 1 94.22740524781341 83.88704318936877 2 3.883381924198251 6.914451827242525 3 0.80466472303207 2.1490863787375414 4 0.3848396501457726 1.3704318936877076 5 0.163265306122449 0.7267441860465116 6 0.11661807580174927 0.6229235880398671 7 0.11661807580174927 0.7267441860465116 8 0.05830903790087463 0.41528239202657813 9 0.04664723032069971 0.37375415282392027 >10 0.19825072886297374 2.8135382059800667 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC 40 0.41528239202657813 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.24916943521594684 No Hit TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG 23 0.2387873754152824 RNA PCR Primer, Index 31 (96% over 29bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.1972591362126246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.18687707641196014 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 17 0.17649501661129569 TruSeq Adapter, Index 1 (96% over 28bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.16611295681063123 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 16 0.16611295681063123 No Hit CGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAA 15 0.15573089700996678 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 12 0.12458471760797342 No Hit GTACTTTGTGTCACAGCTGAAGAAGATGAAGAAGTCATCCGGGGAAATTG 11 0.11420265780730898 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 10 0.10382059800664453 RNA PCR Primer, Index 33 (96% over 29bp) CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA 10 0.10382059800664453 No Hit CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG 10 0.10382059800664453 No Hit GTCCCGCTTCTGGTACTTTGTGTCACAGCTGAAGAAGATGAAGAAGTCAT 10 0.10382059800664453 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.10382059800664453 No Hit GTATAGGAAGACGCTGGGTTGGTCCGATACTATTTACACGTGGTGGCAGC 10 0.10382059800664453 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.005191029900332226 24-25 0.0 0.0 0.0 0.0 0.010382059800664452 26-27 0.0 0.0 0.0 0.0 0.010382059800664452 28-29 0.0 0.0 0.0 0.005191029900332226 0.010382059800664452 30-31 0.0 0.0 0.0 0.02595514950166113 0.010382059800664452 32-33 0.0 0.0 0.0 0.04152823920265781 0.010382059800664452 34-35 0.0 0.0 0.0 0.09343853820598007 0.010382059800664452 36-37 0.0 0.0 0.0 0.1401578073089701 0.010382059800664452 38-39 0.0 0.0 0.0 0.20764119601328904 0.010382059800664452 40-41 0.0 0.0 0.0 0.2647425249169435 0.010382059800664452 42-43 0.0 0.0 0.0 0.3426079734219269 0.010382059800664452 44-45 0.0 0.0 0.0 0.4049003322259136 0.010382059800664452 46-47 0.0 0.0 0.0 0.49314784053156147 0.010382059800664452 48-49 0.0 0.0 0.0 0.6488787375415282 0.010382059800664452 50-51 0.0 0.0 0.0 0.7630813953488371 0.010382059800664452 52-53 0.0 0.0 0.0 0.9032392026578073 0.010382059800664452 54-55 0.0 0.0 0.0 1.0070598006644518 0.010382059800664452 56-57 0.0 0.0 0.0 1.1316445182724253 0.010382059800664452 58-59 0.0 0.0 0.0 1.3496677740863787 0.010382059800664452 60-61 0.0 0.0 0.0 1.5209717607973423 0.010382059800664452 62-63 0.0 0.0 0.0 1.7286129568106312 0.010382059800664452 64-65 0.0 0.0 0.0 1.8272425249169435 0.010382059800664452 66-67 0.0 0.0 0.0 2.008928571428571 0.010382059800664452 68-69 0.0 0.0 0.0 2.1958056478405314 0.010382059800664452 70-71 0.0 0.0 0.0 2.3463455149501664 0.010382059800664452 72-73 0.0 0.0 0.0 2.5591777408637872 0.010382059800664452 74-75 0.0 0.0 0.0 2.7460548172757475 0.010382059800664452 76-77 0.0 0.0 0.0 2.9744601328903655 0.010382059800664452 78-79 0.0 0.0 0.0 3.234011627906977 0.010382059800664452 80-81 0.0 0.0 0.0 3.488372093023256 0.010382059800664452 82-83 0.0 0.0 0.0 3.7427325581395348 0.010382059800664452 84-85 0.0 0.0 0.0 3.9970930232558137 0.010382059800664452 86-87 0.0 0.0 0.0 4.3085548172757475 0.010382059800664452 88 0.0 0.0 0.0 4.474667774086379 0.010382059800664452 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCAGA 20 0.0021142613 70.36328 7 GTATCAA 20 0.0021142613 70.36328 1 TCAACGC 20 0.0021142613 70.36328 4 TATCAAC 20 0.0021142613 70.36328 2 ATCAACG 20 0.0021142613 70.36328 3 GGGGAAT 20 0.0021142613 70.36328 9 CAACGCA 20 0.0021142613 70.36328 5 TGGGGGG 30 1.3159614E-4 62.54514 6 ATGGGGG 25 0.0051189107 56.290623 5 AACGCAG 25 0.0051189107 56.290623 6 CGCAGAG 25 0.0051189107 56.290623 8 TACATGG 45 9.737445E-4 41.696762 2 ACATGGG 50 0.001634868 37.52708 3 GTACATG 55 0.0026103386 34.115532 1 >>END_MODULE