FastQCFastQC Report
Mon 6 Jun 2016
ERR1211330_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211330_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12109570
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409120.3378484950332671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316570.26142133866024975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180840.149336433911361No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203850.052.3284721
GTATCAA381850.043.553711
CAACGCA471550.034.359635
ATCAACG479450.033.8825263
TATCAAC488650.033.6968352
TCAACGC501400.032.5486534
AACGCAG506650.032.248616
ACGCAGA568500.028.435587
GTGGTAT90000.028.0109581
CGCAGAG587350.027.4655088
GTACTAG48100.026.0101131
GTACATG525950.025.8350181
GCAGAGT626900.025.0218039
TACATGG540050.024.828942
ACATGGG551900.023.5340883
TGGTATC105200.023.2031272
GAGTACT328800.022.35892712-13
CATGGGG390550.021.6575054
CAGAGTA611550.020.83215910-11
TAGTACT69800.020.6678624
Produced by FastQC (version 0.11.5)