##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211330_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12109570 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.246482823089508 31.0 31.0 34.0 28.0 34.0 2 31.262141512869572 31.0 31.0 34.0 28.0 34.0 3 31.30768805168144 31.0 31.0 34.0 28.0 34.0 4 34.91189678906848 37.0 35.0 37.0 32.0 37.0 5 34.95276298002324 37.0 35.0 37.0 32.0 37.0 6 35.01851898952646 37.0 35.0 37.0 32.0 37.0 7 34.94606298985018 37.0 35.0 37.0 32.0 37.0 8 34.946222780825416 37.0 35.0 37.0 32.0 37.0 9 36.47310490793645 39.0 37.0 39.0 32.0 39.0 10-11 36.40537913402375 39.0 37.0 39.0 32.0 39.0 12-13 36.313219007776496 39.0 37.0 39.0 31.0 39.0 14-15 37.412831834656394 40.0 37.0 41.0 31.0 41.0 16-17 37.411623286375985 40.0 37.0 41.0 31.0 41.0 18-19 37.411907111482904 40.0 37.0 41.0 31.0 41.0 20-21 37.37898092170077 40.0 37.0 41.0 31.0 41.0 22-23 37.25568529683548 40.0 37.0 41.0 31.0 41.0 24-25 37.135896774204205 40.0 36.0 41.0 30.5 41.0 26-27 36.9855071649943 40.0 36.0 41.0 30.0 41.0 28-29 36.848746941468605 39.0 36.0 41.0 30.0 41.0 30-31 36.66239866485763 39.0 36.0 41.0 30.0 41.0 32-33 36.540990637983015 39.0 35.0 41.0 30.0 41.0 34-35 36.3955819240485 39.0 35.0 40.5 29.0 41.0 36-37 36.29522427303364 39.0 35.0 40.5 29.0 41.0 38-39 36.13831742167558 39.0 35.0 40.0 28.0 41.0 40-41 35.96590357873979 38.5 35.0 40.0 27.5 41.0 42-43 35.8102501988097 38.0 35.0 40.0 27.0 41.0 44-45 35.65703819375915 38.0 34.0 40.0 26.5 41.0 46-47 35.47374869627906 38.0 34.0 40.0 26.0 41.0 48-49 35.22454595827928 38.0 34.0 40.0 25.0 41.0 50-51 34.54909104947575 37.5 33.0 39.5 24.0 40.5 52-53 34.792625171661754 38.0 33.5 40.0 24.5 40.5 54-55 35.14627063553867 38.0 34.0 40.0 26.0 41.0 56-57 35.03281000068541 38.0 34.0 40.0 25.5 41.0 58-59 34.792705314887314 37.5 33.5 40.0 25.0 41.0 60-61 34.5185229533336 37.0 33.0 40.0 25.0 41.0 62-63 34.24379982113321 36.5 33.0 40.0 24.0 41.0 64-65 33.95861376580672 36.0 33.0 39.5 23.5 41.0 66-67 33.73179646345824 36.0 32.0 39.0 23.0 41.0 68-69 33.389075623659636 35.0 32.0 39.0 23.0 41.0 70-71 33.050506747968754 35.0 32.0 38.5 22.5 40.0 72-73 32.69209625114682 35.0 31.0 37.5 22.0 40.0 74-75 32.329151035090426 35.0 31.0 37.0 21.0 39.0 76-77 31.98644237574084 35.0 31.0 36.5 20.0 39.0 78-79 31.631038013736244 35.0 30.5 36.0 20.0 39.0 80-81 31.30440469810241 34.0 30.0 35.5 20.0 37.0 82-83 30.999859986770794 34.0 30.0 35.0 19.0 37.0 84-85 30.706858418589594 34.0 29.5 35.0 18.5 36.5 86-87 30.457730951635774 34.0 29.0 35.0 18.0 36.0 88-89 30.202731641173056 34.0 29.0 35.0 18.0 36.0 90-91 29.942136797590667 34.0 29.0 35.0 17.0 35.5 92-93 29.704126859995853 34.0 29.0 35.0 16.0 35.0 94-95 29.483695292235808 34.0 27.5 35.0 15.0 35.0 96-97 29.281539311470183 34.0 27.0 35.0 14.0 35.0 98-99 29.038365317678497 33.0 27.0 35.0 11.0 35.0 100 27.33776038290377 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.35959067190366056 1101 2 -0.378353426842196 1101 3 -0.3903753724650585 1101 4 -0.28467614938710994 1101 5 -0.33034684919287827 1101 6 -0.31722949245785514 1101 7 -0.33799422679553714 1101 8 -0.5609247850327606 1101 9 -0.5452091168879889 1101 10-11 -0.5607542105566452 1101 12-13 -0.48282712520180127 1101 14-15 -0.5763754032141009 1101 16-17 -0.5362108145145683 1101 18-19 -0.5868851966235198 1101 20-21 -0.6497931096988836 1101 22-23 -0.6544072205192251 1101 24-25 -0.6685304630035205 1101 26-27 -0.7220560973177115 1101 28-29 -0.7411389936258459 1101 30-31 -0.7744800426684009 1101 32-33 -0.8391635315010078 1101 34-35 -0.8211589218691699 1101 36-37 -0.8477462644137645 1101 38-39 -0.8987570487251091 1101 40-41 -0.9141561712448549 1101 42-43 -0.932427356165185 1101 44-45 -1.0241196004576878 1101 46-47 -0.9194801377048876 1101 48-49 -0.8941423797666488 1101 50-51 -0.8383952799825565 1101 52-53 -0.7194741214487834 1101 54-55 -0.6094546324972825 1101 56-57 -0.5609694451605378 1101 58-59 -0.7002087523587974 1101 60-61 -0.708520988476316 1101 62-63 -0.709903650191336 1101 64-65 -0.7548017555847224 1101 66-67 -0.8449538530716083 1101 68-69 -0.7573493988020346 1101 70-71 -0.7601256859584495 1101 72-73 -0.7732990229582697 1101 74-75 -0.8817590693776012 1101 76-77 -0.787606504122838 1101 78-79 -0.8922326275126231 1101 80-81 -0.9690076008186104 1101 82-83 -1.0407877597974036 1101 84-85 -1.0743741572222518 1101 86-87 -1.0457214954250063 1101 88-89 -1.0548615871874567 1101 90-91 -1.021281460686783 1101 92-93 -1.0778598577155698 1101 94-95 -1.1016439128194015 1101 96-97 -1.2463823426317369 1101 98-99 -1.2938578707315678 1101 100 -1.358992666867266 1102 1 -0.4308919959015398 1102 2 -0.4651566231645603 1102 3 -0.45086005299522114 1102 4 -0.35443441917787055 1102 5 -0.3290862045599141 1102 6 -0.3767934346403763 1102 7 -0.4507373677063171 1102 8 -0.4291501485797653 1102 9 -0.4942202710648331 1102 10-11 -0.42352831285317194 1102 12-13 -0.4746915803865974 1102 14-15 -0.6063477916660176 1102 16-17 -0.6648106025685436 1102 18-19 -0.6543564974931328 1102 20-21 -0.6758591423113742 1102 22-23 -0.7247674303043823 1102 24-25 -0.7169529385377587 1102 26-27 -0.6803428939086089 1102 28-29 -0.8049021563168921 1102 30-31 -0.6941549914531606 1102 32-33 -0.7300072118021319 1102 34-35 -0.7794094769358395 1102 36-37 -0.8549204117113689 1102 38-39 -0.9379356510291146 1102 40-41 -1.0130846649589245 1102 42-43 -0.9808618729395278 1102 44-45 -0.9731386432876477 1102 46-47 -1.0888605851880016 1102 48-49 -1.1205563082923362 1102 50-51 -1.1351644537010444 1102 52-53 -0.9532420922213021 1102 54-55 -0.8241573737432262 1102 56-57 -0.8108144026240822 1102 58-59 -0.8797388334970648 1102 60-61 -0.9677241225982343 1102 62-63 -0.9437568815266957 1102 64-65 -0.946550451621917 1102 66-67 -0.9177490481540005 1102 68-69 -0.952691992698881 1102 70-71 -1.039033257702279 1102 72-73 -1.0958740678205174 1102 74-75 -1.0141892802516068 1102 76-77 -0.9775728399793593 1102 78-79 -0.9600930430338579 1102 80-81 -1.0142484458157917 1102 82-83 -1.0816287853037991 1102 84-85 -1.0877555301390416 1102 86-87 -1.060418249709155 1102 88-89 -1.2001854881318899 1102 90-91 -1.1936186679167768 1102 92-93 -1.1669504605163787 1102 94-95 -1.1992898040975213 1102 96-97 -1.2411943772748266 1102 98-99 -1.276734099741649 1102 100 -1.3219299569975398 1103 1 -0.42149085471755754 1103 2 -0.49129605673589793 1103 3 -0.4260940135158222 1103 4 -0.370288294832946 1103 5 -0.354336505126021 1103 6 -0.4009297190081824 1103 7 -0.38704552230488787 1103 8 -0.4219987295122891 1103 9 -0.5294394031439253 1103 10-11 -0.5723722182558575 1103 12-13 -0.6412968156721277 1103 14-15 -0.7000075544280762 1103 16-17 -0.7086692576655338 1103 18-19 -0.6930713807324267 1103 20-21 -0.8203474883238684 1103 22-23 -0.8057961624212808 1103 24-25 -0.7321044533002095 1103 26-27 -0.76086798199848 1103 28-29 -0.7435089290560413 1103 30-31 -0.8113509335068585 1103 32-33 -0.8850144642487265 1103 34-35 -0.9429256775474286 1103 36-37 -0.9930085741743966 1103 38-39 -1.0819764603997655 1103 40-41 -1.010675745091568 1103 42-43 -0.9309497873488439 1103 44-45 -0.9822648882424758 1103 46-47 -1.0084928117635812 1103 48-49 -0.9515893073095327 1103 50-51 -0.9575629455550754 1103 52-53 -0.8525521682517336 1103 54-55 -0.8649405823392726 1103 56-57 -0.8305501988961552 1103 58-59 -0.7510265403561291 1103 60-61 -0.6976635283341395 1103 62-63 -0.8157963289130379 1103 64-65 -0.8151758309566333 1103 66-67 -0.8330119559768079 1103 68-69 -0.7819138016694538 1103 70-71 -0.7798473360595182 1103 72-73 -0.8919792181104178 1103 74-75 -0.8018852987414462 1103 76-77 -0.7462252415894319 1103 78-79 -0.7247526316961093 1103 80-81 -0.8300022953986961 1103 82-83 -0.9366324641678929 1103 84-85 -0.9055698389174509 1103 86-87 -0.9884871353694109 1103 88-89 -1.0238757894033022 1103 90-91 -0.994822794030771 1103 92-93 -1.1002013189893276 1103 94-95 -1.2535876212420547 1103 96-97 -1.2744964899083264 1103 98-99 -1.3248362792075596 1103 100 -1.2809315331400128 1104 1 -0.4048821018406521 1104 2 -0.4004872087689364 1104 3 -0.45035929712691214 1104 4 -0.26763012607279535 1104 5 -0.1980674824779527 1104 6 -0.19348215146254688 1104 7 -0.2375060744521491 1104 8 -0.26914773887836674 1104 9 -0.3766993297433885 1104 10-11 -0.35707430723273603 1104 12-13 -0.47476581012803365 1104 14-15 -0.5351132715180142 1104 16-17 -0.5041384599534098 1104 18-19 -0.5796990299758349 1104 20-21 -0.5638036723238926 1104 22-23 -0.47146019472289424 1104 24-25 -0.5931041352193915 1104 26-27 -0.6567905973272588 1104 28-29 -0.6103035852649228 1104 30-31 -0.6967979775792941 1104 32-33 -0.6675914722725693 1104 34-35 -0.6624825939454126 1104 36-37 -0.7114208608162045 1104 38-39 -0.6834209852574347 1104 40-41 -0.7911458520282793 1104 42-43 -0.7706989981037182 1104 44-45 -0.7316190351384009 1104 46-47 -0.8064636212485041 1104 48-49 -0.8080655737452247 1104 50-51 -0.6544137190665964 1104 52-53 -0.6198814946512741 1104 54-55 -0.6446570227161814 1104 56-57 -0.5719730200350526 1104 58-59 -0.6125810799705746 1104 60-61 -0.5415060244591459 1104 62-63 -0.6249197550972454 1104 64-65 -0.6215213803132542 1104 66-67 -0.6088186474240089 1104 68-69 -0.5673428221092038 1104 70-71 -0.5843041636067454 1104 72-73 -0.621669153980605 1104 74-75 -0.6775835609902252 1104 76-77 -0.656874504922218 1104 78-79 -0.6090288623268236 1104 80-81 -0.709438128375588 1104 82-83 -0.738322622741439 1104 84-85 -0.776072502685544 1104 86-87 -0.8339518837572228 1104 88-89 -0.6946530969738482 1104 90-91 -0.695657681815046 1104 92-93 -0.7528601887103044 1104 94-95 -0.7834067536326828 1104 96-97 -0.7617218047500209 1104 98-99 -0.836146348918323 1104 100 -0.8461475662578444 1105 1 -0.3864919502571631 1105 2 -0.4634513878027029 1105 3 -0.4165863088785642 1105 4 -0.26738215395511133 1105 5 -0.2898083339665476 1105 6 -0.2919020449539218 1105 7 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70-71 -0.4766910140722018 1105 72-73 -0.6095308976417257 1105 74-75 -0.6503720723213746 1105 76-77 -0.6730730517274814 1105 78-79 -0.6558064092022136 1105 80-81 -0.6765054678576838 1105 82-83 -0.712238021218532 1105 84-85 -0.7175843717602817 1105 86-87 -0.7368213723310078 1105 88-89 -0.7954241487587161 1105 90-91 -0.7581722731395217 1105 92-93 -0.8005749374744049 1105 94-95 -0.7909941796093136 1105 96-97 -0.996761424284518 1105 98-99 -0.9697068344586164 1105 100 -0.9564761610809427 1106 1 -0.46727019770194644 1106 2 -0.5006695158710386 1106 3 -0.4582769066528556 1106 4 -0.36696242710620197 1106 5 -0.33843921378100816 1106 6 -0.3858424537572276 1106 7 -0.4012607184704251 1106 8 -0.41974119475604965 1106 9 -0.4664677172181513 1106 10-11 -0.5321260699387764 1106 12-13 -0.5299027753331771 1106 14-15 -0.6196161901723727 1106 16-17 -0.6990381334205296 1106 18-19 -0.6711652040042466 1106 20-21 -0.6806128863041536 1106 22-23 -0.6414425863812738 1106 24-25 -0.6893388211167917 1106 26-27 -0.5835000383885927 1106 28-29 -0.7276178636802015 1106 30-31 -0.7065592209302665 1106 32-33 -0.8034157120429697 1106 34-35 -0.7890249453334448 1106 36-37 -0.8410945012170359 1106 38-39 -0.8707508046060113 1106 40-41 -0.849127097352067 1106 42-43 -0.8692724029026735 1106 44-45 -0.8524398121389609 1106 46-47 -0.9005701284658798 1106 48-49 -0.9700521146865526 1106 50-51 -0.9396284787564113 1106 52-53 -0.7473339309461267 1106 54-55 -0.8306011618361495 1106 56-57 -0.7012344187397161 1106 58-59 -0.7344008022828987 1106 60-61 -0.7312311203559645 1106 62-63 -0.774773868083301 1106 64-65 -0.7707052667054626 1106 66-67 -0.7952875752148643 1106 68-69 -0.7417392895037267 1106 70-71 -0.7122857523063146 1106 72-73 -0.7589984924441211 1106 74-75 -0.779973496137508 1106 76-77 -0.7573703848500664 1106 78-79 -0.6957698200384286 1106 80-81 -0.8250787956405681 1106 82-83 -0.7836905312069931 1106 84-85 -0.7472742559050332 1106 86-87 -0.8197637874349155 1106 88-89 -0.8475727448960377 1106 90-91 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0.9559924734821337 2311 30-31 0.9547468415732965 2311 32-33 0.9783587095549606 2311 34-35 1.00930799469252 2311 36-37 1.0343122687818678 2311 38-39 1.0131770344674536 2311 40-41 1.0387165674530507 2311 42-43 1.083392215254996 2311 44-45 1.121853529327531 2311 46-47 1.1579115023818716 2311 48-49 1.1887839635968191 2311 50-51 1.147165993777783 2311 52-53 1.0458559644061722 2311 54-55 1.0093708999047024 2311 56-57 0.9924984600554296 2311 58-59 1.0035733827372653 2311 60-61 0.9517024626388633 2311 62-63 0.9328170941068876 2311 64-65 0.9928463852144915 2311 66-67 1.0174137846655 2311 68-69 0.9806804717631081 2311 70-71 0.9614942375951827 2311 72-73 0.8976611955159868 2311 74-75 0.8929545119753541 2311 76-77 0.9536317679031221 2311 78-79 0.9249343525646658 2311 80-81 0.9477519132394754 2311 82-83 0.9181596827300673 2311 84-85 0.9929612583504195 2311 86-87 1.0537415988981493 2311 88-89 1.084708604640742 2311 90-91 1.1249318118849736 2311 92-93 1.1357186162781012 2311 94-95 1.1938494651988591 2311 96-97 1.2747086776721197 2311 98-99 1.266609675734692 2311 100 1.2589818545991918 2312 1 0.4658680798708801 2312 2 0.480625278129029 2312 3 0.5389983395829816 2312 4 0.43482736339718997 2312 5 0.45803344537229407 2312 6 0.45982694028920434 2312 7 0.5038079331070762 2312 8 0.5012935736857287 2312 9 0.594489963086275 2312 10-11 0.6376801829952967 2312 12-13 0.5855941809833283 2312 14-15 0.7330637816993075 2312 16-17 0.7599854017724823 2312 18-19 0.714860359450114 2312 20-21 0.7896100932963535 2312 22-23 0.7797708576142384 2312 24-25 0.8288077251241361 2312 26-27 0.880763591676164 2312 28-29 0.8635285269380049 2312 30-31 0.8961726682327793 2312 32-33 0.8391198535616979 2312 34-35 0.835666472880348 2312 36-37 0.881471472164371 2312 38-39 0.9412243964927782 2312 40-41 0.906502151196797 2312 42-43 0.9023825640775485 2312 44-45 0.906108460287335 2312 46-47 0.9560153926797668 2312 48-49 0.9361140322690886 2312 50-51 0.9076050528398483 2312 52-53 0.7928882156530648 2312 54-55 0.8034213240335006 2312 56-57 0.7970143089960686 2312 58-59 0.8134273243617116 2312 60-61 0.7783436163284421 2312 62-63 0.7452057007836288 2312 64-65 0.7779488227175477 2312 66-67 0.8202143209931663 2312 68-69 0.8649231389727774 2312 70-71 0.8306049366116071 2312 72-73 0.8381949065693206 2312 74-75 0.8158096122747054 2312 76-77 0.837411991941611 2312 78-79 0.8113517923421689 2312 80-81 0.8059881821854553 2312 82-83 0.8673110075853572 2312 84-85 0.8610261824861283 2312 86-87 0.9426339842542788 2312 88-89 0.930660091240977 2312 90-91 0.9641140681765528 2312 92-93 1.0063817222380074 2312 94-95 1.0295030860540209 2312 96-97 1.0072713240328852 2312 98-99 1.0697101802396354 2312 100 1.142640898605464 2313 1 0.49993764223501813 2313 2 0.5242607586157284 2313 3 0.5309058788855552 2313 4 0.4383777584893167 2313 5 0.4821461302727599 2313 6 0.4811951022743699 2313 7 0.5213397050598161 2313 8 0.438583605846496 2313 9 0.5828843960484491 2313 10-11 0.6078303498577711 2313 12-13 0.6406828307744945 2313 14-15 0.809462333704829 2313 16-17 0.7898437503200881 2313 18-19 0.7651395202012807 2313 20-21 0.7679494092778256 2313 22-23 0.7053941481560742 2313 24-25 0.7634699621119552 2313 26-27 0.808780467178579 2313 28-29 0.8253335919205824 2313 30-31 0.8909294398055465 2313 32-33 0.9202634148394395 2313 34-35 0.9047415848350937 2313 36-37 0.9263733856748715 2313 38-39 0.9899670722511544 2313 40-41 0.9930371329802767 2313 42-43 0.8346816134121653 2313 44-45 0.9252328117160218 2313 46-47 1.0210848109689934 2313 48-49 1.0650560841747776 2313 50-51 0.9550993673982617 2313 52-53 0.8693100392958044 2313 54-55 0.8509441777077384 2313 56-57 0.8314190381092175 2313 58-59 0.8222589653353651 2313 60-61 0.8471518357603642 2313 62-63 0.856911178149403 2313 64-65 0.8472280598783826 2313 66-67 0.83984152404323 2313 68-69 0.8583877491158631 2313 70-71 0.8382947162665175 2313 72-73 0.8448416838197303 2313 74-75 0.9039618453212839 2313 76-77 0.9071891255463207 2313 78-79 0.9263147471853053 2313 80-81 0.9219743187230769 2313 82-83 0.9631910273311171 2313 84-85 0.9185244434894848 2313 86-87 0.9960422302942469 2313 88-89 0.9411188365775409 2313 90-91 0.9609423746072281 2313 92-93 0.9957402807560491 2313 94-95 1.0694429826656986 2313 96-97 1.1201644981093146 2313 98-99 1.1226161836453876 2313 100 1.1592357047882764 2314 1 0.34376527139475854 2314 2 0.26875133608752577 2314 3 0.3281889308868884 2314 4 0.27342992406205724 2314 5 0.25518724429571193 2314 6 0.3196128087171246 2314 7 0.39039406154381595 2314 8 0.34757823187860737 2314 9 0.44086556260680965 2314 10-11 0.49040893749025116 2314 12-13 0.45780627859883083 2314 14-15 0.6395194579667063 2314 16-17 0.6009209137176015 2314 18-19 0.6113486191666127 2314 20-21 0.6339877885332825 2314 22-23 0.6811938552195045 2314 24-25 0.702912966936978 2314 26-27 0.7793473104507882 2314 28-29 0.6810249281421221 2314 30-31 0.7207023029080233 2314 32-33 0.7827501521034961 2314 34-35 0.8062006335207172 2314 36-37 0.8185205418814263 2314 38-39 0.8527303529696084 2314 40-41 0.8772963463917804 2314 42-43 0.7660033052879669 2314 44-45 0.8518611863398391 2314 46-47 0.6566557132955566 2314 48-49 0.5919363616331523 2314 50-51 0.7778587724533779 2314 52-53 0.7029066355179054 2314 54-55 0.6867855715853679 2314 56-57 0.7019089030050623 2314 58-59 0.6642413701905809 2314 60-61 0.5477594611177921 2314 62-63 0.7167339113804871 2314 64-65 0.7135526870291784 2314 66-67 0.7362265051753454 2314 68-69 0.7246002071931841 2314 70-71 0.7495381541795965 2314 72-73 0.7442434827225171 2314 74-75 0.7885912640512558 2314 76-77 0.7578997555209206 2314 78-79 0.7352290632376999 2314 80-81 0.8020995268973543 2314 82-83 0.8019070803816533 2314 84-85 0.8489077745100531 2314 86-87 0.8248589690845236 2314 88-89 0.6957014111305746 2314 90-91 0.7495550409759879 2314 92-93 0.9456559208915358 2314 94-95 0.9707698879403708 2314 96-97 0.9739759644080586 2314 98-99 1.006352355708536 2314 100 1.0873612836161506 2315 1 0.3171490670053352 2315 2 0.3466105065456624 2315 3 0.3475629311806756 2315 4 0.21458336986051307 2315 5 0.2809132346130454 2315 6 0.2702352334057707 2315 7 0.27888375718053027 2315 8 0.2691536363612457 2315 9 0.199475342927542 2315 10-11 0.30998320213116415 2315 12-13 0.29228524857939675 2315 14-15 0.48443337819196586 2315 16-17 0.301107034392075 2315 18-19 0.4646046434785376 2315 20-21 0.3149688635700798 2315 22-23 0.3650641242741415 2315 24-25 0.5447155507357877 2315 26-27 0.6023509945443593 2315 28-29 0.4890755041051591 2315 30-31 0.581777331659282 2315 32-33 0.6071668180116419 2315 34-35 0.4802779191746822 2315 36-37 0.6415993471064354 2315 38-39 0.678172697903527 2315 40-41 0.7319966923824737 2315 42-43 0.7696542159116717 2315 44-45 0.751290292393854 2315 46-47 0.7643844245286218 2315 48-49 0.7212172062927777 2315 50-51 0.6940246885708561 2315 52-53 0.3762360817090098 2315 54-55 0.5576458040887431 2315 56-57 0.5488348857589784 2315 58-59 0.5313884900837138 2315 60-61 0.6538659169839818 2315 62-63 0.6571421880414903 2315 64-65 0.666400765076304 2315 66-67 0.5876221650768727 2315 68-69 0.6197922938573583 2315 70-71 0.542684791704346 2315 72-73 0.6656568195747141 2315 74-75 0.38185885575014566 2315 76-77 0.6046678898591118 2315 78-79 0.6103281808384757 2315 80-81 0.6157596594470718 2315 82-83 0.6076061607587988 2315 84-85 0.6095505399372527 2315 86-87 0.6322955638171806 2315 88-89 0.5255023406351391 2315 90-91 0.6670334446564716 2315 92-93 0.7710551031081572 2315 94-95 0.7549320295956505 2315 96-97 0.8112919640272729 2315 98-99 0.9354534914919661 2315 100 0.9580644094608424 2316 1 0.08258354487840336 2316 2 0.10318491211670278 2316 3 0.09615276672162665 2316 4 -0.007257170026470305 2316 5 -0.004883097698744621 2316 6 -0.16477778592506098 2316 7 -0.10909755602507687 2316 8 -0.06783643900720193 2316 9 0.009938590963216143 2316 10-11 0.06858050928775583 2316 12-13 0.04197636508889957 2316 14-15 -0.09211634796309198 2316 16-17 0.06085353849949371 2316 18-19 0.11340128596216204 2316 20-21 0.19109999086318652 2316 22-23 0.08505862436147282 2316 24-25 0.024905891119828993 2316 26-27 -0.06573876040798865 2316 28-29 -0.023869858877283434 2316 30-31 -0.5915443569179786 2316 32-33 -0.4226939481531389 2316 34-35 -0.44358310090824915 2316 36-37 -0.18664110853918459 2316 38-39 -0.1208500036375213 2316 40-41 0.04450396235010601 2316 42-43 0.06698951346495363 2316 44-45 -0.06688450668274726 2316 46-47 -0.23098603184371314 2316 48-49 -0.12785299623604374 2316 50-51 0.05197174471073396 2316 52-53 -0.29903658783761955 2316 54-55 -0.1562620451244996 2316 56-57 -0.22306022716682605 2316 58-59 -0.6027105645981194 2316 60-61 -0.09880100263268332 2316 62-63 -0.05597465037038063 2316 64-65 -0.4435383882570392 2316 66-67 -0.7155748290962052 2316 68-69 -0.7291140866887957 2316 70-71 -0.4704453320785831 2316 72-73 -0.7185235195727202 2316 74-75 -0.4943573176841909 2316 76-77 -0.2223728030403329 2316 78-79 -0.15282155055266955 2316 80-81 -0.1732530366418672 2316 82-83 0.038388711924799424 2316 84-85 0.07384047404217853 2316 86-87 0.16083806184694183 2316 88-89 0.155950734489398 2316 90-91 -0.45608538472055926 2316 92-93 -0.4525997166401119 2316 94-95 -0.09678339736290553 2316 96-97 0.172135760012619 2316 98-99 0.27460640429255534 2316 100 0.05945294882316787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 321.0 1 918.0 2 533.0 3 461.0 4 462.0 5 406.0 6 478.0 7 1050.0 8 2671.0 9 5171.0 10 8903.0 11 15598.0 12 24301.0 13 32097.0 14 38161.0 15 43914.0 16 51095.0 17 60459.0 18 71755.0 19 84521.0 20 102013.0 21 123314.0 22 137946.0 23 142124.0 24 144818.0 25 151210.0 26 165663.0 27 186671.0 28 217871.0 29 257983.0 30 309595.0 31 377860.0 32 472281.0 33 607086.0 34 824545.0 35 1134428.0 36 1653537.0 37 2270286.0 38 1945606.0 39 441220.0 40 238.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.08020343428339 19.116989415780626 13.527833032696282 23.274974117239694 2 15.754115577771465 19.769067005637126 40.322890525614746 24.153926890976667 3 19.268627627664582 25.26656263805932 29.12784211724783 26.33696761702826 4 12.994374127345507 16.304012693006086 37.772719370007984 32.92889380964043 5 13.894919508606247 37.46126167126242 34.06014357578283 14.583675244348504 6 31.485976304292883 36.4542724504833 17.607893028192468 14.45185821703134 7 28.25971345290187 32.15262110777601 21.49759222413189 18.090073215190234 8 26.598149015564758 34.75982060172277 19.617403185979903 19.024627196732567 9 26.626822474853483 16.069639855888827 20.352324624510167 36.95121304474752 10-11 25.46445362225354 25.969944481134476 27.24568922628024 21.319912670331743 12-13 25.97482582991781 23.556134458849105 27.77093273051024 22.69810698072285 14-15 23.446511339174563 24.978957472720918 25.41648178691254 26.15804940119198 16-17 22.641949836346363 28.040223247333216 26.213280161425658 23.10454675489476 18-19 22.387888627335716 27.167942538652962 28.45087344536984 21.99329538864148 20-21 23.37289174334544 26.178428576834122 28.345323075311395 22.103356604509035 22-23 23.44926080605924 25.930919393621664 28.085961773754814 22.53385802656428 24-25 23.019706011260578 26.171390305220232 28.340434821985166 22.468468861534028 26-27 22.844826108193278 26.362558353277603 28.07199328975264 22.720622248776483 28-29 22.589331287890417 26.4264535321317 28.332142508369785 22.652072671608096 30-31 23.0402006427929 26.28801786465741 28.16328144192551 22.508500050624185 32-33 22.973243646635755 26.50329732490166 27.85023355285483 22.67322547560776 34-35 23.120356688293803 26.461558340144997 28.082131233705088 22.335953737856112 36-37 23.059762653177664 26.275951999274998 27.954636581240187 22.70964876630715 38-39 23.04797660244555 26.32452895782784 28.102259130344407 22.525235309382197 40-41 22.964503529833827 26.050944933419046 28.265345710215072 22.71920582653205 42-43 22.864605781939527 26.139190960099445 27.999273561124085 22.996929696836947 44-45 22.814256318268374 26.486260175664206 27.90597502632831 22.793508479739106 46-47 22.894681774266417 26.42546177698904 27.581713306915486 23.098143141829063 48-49 23.170586201183813 26.388088658338027 27.482242180589576 22.959082959888587 50-51 23.04898998367897 26.524067621868504 27.300154138240572 23.126788256211952 52-53 22.871673704519583 26.76125288683078 27.204210829355528 23.16286257929411 54-55 22.789285988853024 26.66819350285759 27.260623295347912 23.281897212941473 56-57 23.059775012861525 26.594435567138397 27.053269661298103 23.292519758701975 58-59 22.836833480319182 26.573686343932344 27.394613460268296 23.19486671548018 60-61 22.913845916920334 26.748742291235878 27.192538415348093 23.144873376495692 62-63 22.904461957372455 26.576123241556193 27.14758507511632 23.371829725955035 64-65 22.63158577459303 26.502386219366276 27.27489385905236 23.591134146988338 66-67 22.855632076216065 26.362051921409414 27.147495122272638 23.634820880101888 68-69 22.58205766479803 26.48526502967634 27.249401974214265 23.683275331311368 70-71 22.703373812428246 26.22125877328103 27.28982805284276 23.78553936144796 72-73 22.854799151154666 26.415214930853146 27.14531033196314 23.584675586029043 74-75 22.83903592289581 26.58257523603208 27.044710442048032 23.533678399024083 76-77 22.70890547637532 26.45584193478306 26.977949936441593 23.85730265240003 78-79 22.650958561820556 26.39309483488394 27.069031406982752 23.88691519631275 80-81 22.528396025126625 26.634741124398474 27.05684129740803 23.780021553066867 82-83 22.864360697701454 26.29796577689321 27.346766922163575 23.490906603241765 84-85 22.90140860082807 26.374031015518167 26.95685630381064 23.767704079843117 86-87 22.50537770916468 26.580584211172308 27.187794697821886 23.726243381841126 88-89 22.7358032354604 26.12639891958515 27.143163231123935 23.99463461383052 90-91 22.765602476112555 26.092138104648072 27.15560284673118 23.98665657250819 92-93 22.81241858759484 26.15121505270824 27.08652001673441 23.94984634296251 94-95 22.476484209586804 25.99199363986916 27.289192570811828 24.242329579732207 96-97 22.58174172021604 25.892527290005468 27.20254506594941 24.323185923829083 98-99 22.4380271567766 26.024176731282527 27.10949488818673 24.428301223754147 100 22.762796144010327 25.79706998165338 26.715599801460638 24.724534072875652 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1833.0 1 1412.0 2 1396.5 3 1819.5 4 1542.0 5 1477.0 6 2286.5 7 4170.5 8 7016.5 9 8811.5 10 8110.0 11 7103.5 12 7544.5 13 8543.5 14 9579.5 15 10533.0 16 11547.5 17 12705.5 18 13742.0 19 14684.0 20 15862.5 21 17176.5 22 19198.0 23 22198.5 24 26107.0 25 31212.5 26 38366.5 27 47208.0 28 58291.5 29 75150.0 30 97186.0 31 119730.0 32 148641.5 33 180916.5 34 210728.5 35 239151.5 36 273473.5 37 316198.5 38 340406.0 39 344691.0 40 342656.5 41 341672.0 42 351229.5 43 371206.0 44 395793.5 45 430446.5 46 462715.5 47 484847.0 48 505321.0 49 515162.0 50 508636.0 51 501262.0 52 491891.5 53 477252.5 54 461010.5 55 427868.5 56 387247.0 57 340638.0 58 296466.5 59 262646.0 60 223847.5 61 183368.5 62 147731.0 63 116553.0 64 87366.0 65 62152.0 66 45023.5 67 33794.5 68 25593.5 69 18820.0 70 14341.5 71 10685.5 72 7611.5 73 5654.5 74 4250.5 75 3076.5 76 2215.0 77 1414.0 78 858.5 79 592.0 80 440.0 81 311.5 82 227.0 83 172.5 84 134.0 85 107.0 86 86.5 87 74.5 88 59.5 89 47.5 90 40.0 91 31.5 92 24.0 93 17.5 94 11.0 95 9.0 96 7.5 97 7.0 98 6.0 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0252362387764388 2 0.1101112591115952 3 0.016540636868195978 4 0.017465525200316776 5 0.019612587399882902 6 0.04559204001463305 7 0.046748150429784044 8 0.03848196096145445 9 0.03233805989808061 10-11 0.036380317385340685 12-13 0.035550395265893014 14-15 0.030789697734931957 16-17 0.0365908946395289 18-19 0.0356701352731765 20-21 0.0463435117844812 22-23 0.04631460902410242 24-25 0.06508901637300087 26-27 0.056868245528123626 28-29 0.048853922971666214 30-31 0.04683485871092037 32-33 0.042416865338736225 34-35 0.044031290954179216 36-37 0.03959265275315308 38-39 0.04458457236714433 40-41 0.053854100517194255 42-43 0.05527446474152261 44-45 0.05866847460314446 46-47 0.060055807101325645 48-49 0.05908550014575249 50-51 0.05345771980342819 52-53 0.06301627555726587 54-55 0.05046009065557241 56-57 0.054597314355505605 58-59 0.05255347630014938 60-61 0.048011613954913344 62-63 0.054291770888644265 64-65 0.05222315903867768 66-67 0.05024951340138419 68-69 0.05640993032783163 70-71 0.05768578075026612 72-73 0.059849358812905824 74-75 0.05018757891485825 76-77 0.05066653894399223 78-79 0.06048934850700727 80-81 0.0606792809323535 82-83 0.05142213967960878 84-85 0.056868245528123626 86-87 0.054506477108600876 88-89 0.062363898965859234 90-91 0.0675622668682703 92-93 0.07231883543346296 94-95 0.07589039082312585 96-97 0.07155910573207802 98-99 0.08054786420987699 100 0.12587565041533266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.210957E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.357320541844054 #Duplication Level Percentage of deduplicated Percentage of total 1 70.92954623217474 35.71821895501171 2 14.752109639515037 14.857534275709728 3 5.8316750623705245 8.810025912350127 4 2.846012557523199 5.732702665012366 5 1.5983492407982056 4.024429252834418 6 0.9841319912089955 2.9734950082076788 7 0.6348711268108568 2.237928618490323 8 0.4622579967810287 1.8622459293546383 9 0.32976221495056124 1.4945347404768505 >10 1.506973273730104 13.062798707009279 >50 0.0809712781187763 2.7832047662319233 >100 0.04030412796918536 3.7201481444237507 >500 0.002058799516300324 0.7168412005303971 >1k 8.70895455191192E-4 0.6966718656914516 >5k 2.6390769310767434E-5 0.10447020824520996 >10k+ 7.917230793230229E-5 1.2047497504201778 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40912 0.3378484950332671 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31657 0.26142133866024975 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18084 0.149336433911361 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 8.257931536792801E-6 0.0 0.0 0.0 3 0.0 8.257931536792801E-6 0.0 0.0 0.0 4 0.0 8.257931536792801E-6 0.0 8.257931536792801E-6 8.257931536792801E-6 5 0.0 8.257931536792801E-6 0.0 8.257931536792801E-6 8.257931536792801E-6 6 0.0 8.257931536792801E-6 0.0 8.257931536792801E-6 2.4773794610378403E-5 7 0.0 8.257931536792801E-6 0.0 1.6515863073585602E-5 3.3031726147171204E-5 8 0.0 8.257931536792801E-6 0.0 1.6515863073585602E-5 3.3031726147171204E-5 9 0.0 8.257931536792801E-6 0.0 4.128965768396401E-5 6.606345229434241E-5 10-11 0.0 8.257931536792801E-6 0.0 5.3676554989153205E-5 6.606345229434241E-5 12-13 0.0 8.257931536792801E-6 0.0 7.43213838311352E-5 6.606345229434241E-5 14-15 0.0 1.2386897305189202E-5 0.0 7.43213838311352E-5 9.496621267311722E-5 16-17 0.0 1.6515863073585602E-5 0.0 1.0735310997830641E-4 1.1561104151509922E-4 18-19 0.0 1.6515863073585602E-5 0.0 1.3212690458868482E-4 1.3625587035708122E-4 20-21 0.0 1.6515863073585602E-5 0.0 1.8167449380944163E-4 1.4038483612547763E-4 22-23 0.0 1.6515863073585602E-5 0.0 4.2115450837643283E-4 1.7341656227264882E-4 24-25 0.0 2.8902760378774805E-5 0.0 0.0011519814493825956 2.3122208303019844E-4 26-27 0.0 4.128965768396401E-5 0.0 0.002299833932996795 2.559958776405768E-4 28-29 0.0 4.128965768396401E-5 0.0 0.006321446591414889 2.6425380917736963E-4 30-31 0.0 4.128965768396401E-5 0.0 0.018592732855088993 2.9315656955614447E-4 32-33 0.0 4.9547589220756806E-5 0.0 0.045212175163940584 3.2205932993491926E-4 34-35 0.0 5.3676554989153205E-5 0.0 0.08581229556458239 3.2205932993491926E-4 36-37 0.0 7.845034959953161E-5 0.0 0.15155781749475827 3.30317261471712E-4 38-39 0.0 1.0735310997830641E-4 0.0 0.24474857488746504 3.4683312454529764E-4 40-41 0.0 1.3212690458868482E-4 0.0 0.34453329061230087 3.5509105608209045E-4 42-43 0.0 1.486427676622704E-4 4.1289657683964005E-6 0.4462462333509778 3.5509105608209045E-4 44-45 0.0 1.486427676622704E-4 8.257931536792801E-6 0.5701936567524694 3.5922002185048685E-4 46-47 0.0 1.5277173343066682E-4 8.257931536792801E-6 0.7183326905909954 3.6334898761888326E-4 48-49 0.0 1.6102966496745963E-4 8.257931536792801E-6 0.8763812422736728 3.9638071376605445E-4 50-51 0.0 1.6515863073585603E-4 8.257931536792801E-6 1.0396611935849085 3.9638071376605445E-4 52-53 0.0 1.6515863073585603E-4 8.257931536792801E-6 1.2009344675327034 4.541862345236041E-4 54-55 0.0 1.8167449380944163E-4 8.257931536792801E-6 1.3785378010945062 5.1612072104955E-4 56-57 0.0 1.9819035688302722E-4 8.257931536792801E-6 1.58752540346189 5.202496868179465E-4 58-59 0.0 1.9819035688302722E-4 8.257931536792801E-6 1.8209482252466438 5.285076183547393E-4 60-61 0.0 2.0644828841982004E-4 8.257931536792801E-6 2.0607337832804964 5.532814129651177E-4 62-63 0.0 2.1057725418821641E-4 8.257931536792801E-6 2.3009941723777145 6.193448652594601E-4 64-65 0.0 2.1470621995661282E-4 8.257931536792801E-6 2.5477535535943883 6.193448652594601E-4 66-67 0.0 2.1470621995661282E-4 8.257931536792801E-6 2.825814624301276 6.193448652594601E-4 68-69 0.0 2.2296415149340563E-4 8.257931536792801E-6 3.132811487113085 6.276027967962529E-4 70-71 0.0 2.2296415149340563E-4 8.257931536792801E-6 3.460870204309484 6.606345229434241E-4 72-73 0.0 2.2296415149340563E-4 8.257931536792801E-6 3.788751375977842 7.597297013849377E-4 74-75 0.0 2.2296415149340563E-4 8.257931536792801E-6 4.113684466087566 7.597297013849377E-4 76-77 0.0 2.559958776405768E-4 8.257931536792801E-6 4.47193418098248 7.638586671533341E-4 78-79 0.0 2.6012484340897323E-4 8.257931536792801E-6 4.860767145323905 7.679876329217305E-4 80-81 0.0 2.6425380917736963E-4 8.257931536792801E-6 5.273312760073232 8.216641879108836E-4 82-83 0.0 2.6838277494576604E-4 8.257931536792801E-6 5.689384511588768 9.16630400584001E-4 84-85 0.0 2.7251174071416244E-4 8.257931536792801E-6 6.096727629469915 9.248883321207938E-4 86-87 0.0 2.7664070648255885E-4 8.257931536792801E-6 6.518947411014594 9.331462636575865E-4 88 0.0 2.8076967225095526E-4 8.257931536792801E-6 6.85613114255915 9.414041951943793E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20385 0.0 52.328472 1 GTATCAA 38185 0.0 43.55371 1 CAACGCA 47155 0.0 34.35963 5 ATCAACG 47945 0.0 33.882526 3 TATCAAC 48865 0.0 33.696835 2 TCAACGC 50140 0.0 32.548653 4 AACGCAG 50665 0.0 32.24861 6 ACGCAGA 56850 0.0 28.43558 7 GTGGTAT 9000 0.0 28.010958 1 CGCAGAG 58735 0.0 27.465508 8 GTACTAG 4810 0.0 26.010113 1 GTACATG 52595 0.0 25.835018 1 GCAGAGT 62690 0.0 25.021803 9 TACATGG 54005 0.0 24.82894 2 ACATGGG 55190 0.0 23.534088 3 TGGTATC 10520 0.0 23.203127 2 GAGTACT 32880 0.0 22.358927 12-13 CATGGGG 39055 0.0 21.657505 4 CAGAGTA 61155 0.0 20.832159 10-11 TAGTACT 6980 0.0 20.667862 4 >>END_MODULE