FastQCFastQC Report
Mon 6 Jun 2016
ERR1211330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211330_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12109570
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362700.29951517683947493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296880.2451614714643047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175020.1445303177569476No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA206550.050.1196631
GTATCAA376750.041.8766861
CAACGCA469550.032.968045
ATCAACG473450.032.7036253
TATCAAC485050.032.374022
TCAACGC497700.031.2142584
AACGCAG502050.031.123136
GTGGTAT90500.027.5342851
ACGCAGA569250.027.1441467
CGCAGAG585750.026.3154568
GTACTAG44400.025.366241
GTACATG526400.024.8723131
TACATGG538200.023.9290832
GCAGAGT638400.023.8290189
ACATGGG544300.022.9297543
TGGTATC105950.022.7166252
TAGTACT70050.021.5009844
GAGTACT335300.021.50000612-13
CTAGTAC69400.021.0260183
CATGGGG382050.020.3254074
Produced by FastQC (version 0.11.5)