##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211330_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12109570 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11557099054715 33.0 31.0 34.0 30.0 34.0 2 32.248747973710046 34.0 31.0 34.0 30.0 34.0 3 32.31253892582478 34.0 31.0 34.0 30.0 34.0 4 35.95066670410262 37.0 35.0 37.0 35.0 37.0 5 35.85421034768369 37.0 35.0 37.0 35.0 37.0 6 35.869816186701925 37.0 35.0 37.0 35.0 37.0 7 35.78445122328869 37.0 35.0 37.0 33.0 37.0 8 35.76759992303608 37.0 35.0 37.0 33.0 37.0 9 37.376662837739076 39.0 37.0 39.0 34.0 39.0 10-11 37.33204069178344 39.0 37.0 39.0 34.0 39.0 12-13 37.264157067509416 39.0 37.0 39.0 34.0 39.0 14-15 38.49798638597407 40.0 38.0 41.0 34.0 41.0 16-17 38.48307247903931 40.0 38.0 41.0 34.0 41.0 18-19 38.48841403947456 40.0 38.0 41.0 34.0 41.0 20-21 38.458069939725355 40.0 38.0 41.0 34.0 41.0 22-23 38.349937652616894 40.0 38.0 41.0 34.0 41.0 24-25 38.26007595645427 40.0 38.0 41.0 34.0 41.0 26-27 38.16245618135078 40.0 38.0 41.0 33.0 41.0 28-29 38.062070288210066 40.0 37.5 41.0 33.0 41.0 30-31 38.069450690652104 40.0 37.0 41.0 33.0 41.0 32-33 38.01113189815989 40.0 37.0 41.0 33.0 41.0 34-35 37.884950828146664 40.0 37.0 41.0 33.0 41.0 36-37 37.741987907085054 40.0 37.0 41.0 33.0 41.0 38-39 37.628354887910966 40.0 37.0 41.0 33.0 41.0 40-41 37.51667458051772 40.0 36.5 41.0 32.0 41.0 42-43 37.40727048937328 40.0 36.0 41.0 32.0 41.0 44-45 37.24113585370909 40.0 36.0 41.0 31.0 41.0 46-47 36.99222924513422 39.0 36.0 41.0 31.0 41.0 48-49 35.42588213289159 39.0 35.0 40.5 24.0 41.0 50-51 36.14973000692841 39.0 35.0 40.0 29.0 41.0 52-53 36.237230719175 39.0 35.0 40.0 29.5 41.0 54-55 36.06391515140504 38.0 35.0 40.0 29.0 41.0 56-57 35.83805601685279 38.0 35.0 40.0 28.5 41.0 58-59 35.586426892119206 38.0 34.0 40.0 28.0 41.0 60-61 35.32793633465103 37.0 34.0 40.0 28.0 41.0 62-63 35.004121574919665 37.0 34.0 40.0 27.5 41.0 64-65 34.66490957152071 36.0 33.5 39.0 27.0 41.0 66-67 34.32381096108284 36.0 33.0 39.0 26.0 40.0 68-69 33.96476939313287 35.0 33.0 38.5 26.0 40.0 70-71 33.609828466246114 35.0 32.5 38.0 26.0 40.0 72-73 33.23952997505279 35.0 32.0 37.0 26.0 39.5 74-75 32.886718479681775 35.0 32.0 37.0 25.0 39.0 76-77 31.960491000093313 34.0 30.5 36.0 24.5 38.0 78-79 32.40301203923839 34.5 31.5 36.0 25.0 38.0 80-81 32.28902966827063 35.0 32.0 35.5 25.0 37.0 82-83 32.03103363703253 34.0 31.0 35.0 25.0 37.0 84-85 31.762460475475184 34.0 31.0 35.0 24.0 36.0 86-87 31.512514275899143 34.0 31.0 35.0 24.0 36.0 88-89 31.294289722921622 34.0 31.0 35.0 23.5 36.0 90-91 31.081969508413593 34.0 31.0 35.0 23.0 35.0 92-93 30.896633860657317 34.0 30.0 35.0 22.0 35.0 94-95 30.70639861696163 34.0 30.0 35.0 20.0 35.0 96-97 30.519932375798646 34.0 30.0 35.0 20.0 35.0 98-99 30.30534490489753 34.0 29.0 35.0 19.5 35.0 100 27.860000396380713 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.24898359012280125 1101 2 -0.2492045065356585 1101 3 -0.2600254052629012 1101 4 -0.14998956495054472 1101 5 -0.1529768119649617 1101 6 -0.18583707159881158 1101 7 -0.23145135570614173 1101 8 -0.23867022672580163 1101 9 -0.3353759101800122 1101 10-11 -0.34563001798834847 1101 12-13 -0.4468291670347284 1101 14-15 -0.4975982015515754 1101 16-17 -0.47475000540327983 1101 18-19 -0.4969088281657861 1101 20-21 -0.5615492122173933 1101 22-23 -0.6491898817875921 1101 24-25 -0.5645481757200415 1101 26-27 -0.6663866969653967 1101 28-29 -0.6610610355708886 1101 30-31 -0.5796967519038958 1101 32-33 -0.5295619057100609 1101 34-35 -0.5603536164792047 1101 36-37 -0.5684897983245065 1101 38-39 -0.5717474596743557 1101 40-41 -0.5796877895260621 1101 42-43 -0.5697345055348038 1101 44-45 -0.5400154952077756 1101 46-47 -0.5877819213328337 1101 48-49 0.6523324619909872 1101 50-51 -0.28614948000316787 1101 52-53 -0.6068348593960025 1101 54-55 -0.679161869300394 1101 56-57 -0.6043374818132108 1101 58-59 -0.7502284792867115 1101 60-61 -0.7527449511612474 1101 62-63 -0.6461559411197442 1101 64-65 -0.7913771297224201 1101 66-67 -0.7083717662973328 1101 68-69 -0.7413991024611377 1101 70-71 -0.6781480386602396 1101 72-73 -0.7897130481549297 1101 74-75 -0.836204348182676 1101 76-77 -0.7756350299187282 1101 78-79 -0.7238212768681862 1101 80-81 -0.585114182719181 1101 82-83 -0.560674103148866 1101 84-85 -0.5810877569692394 1101 86-87 -0.6227079541039053 1101 88-89 -0.7114507337035789 1101 90-91 -0.6572691645408035 1101 92-93 -0.7646294973447532 1101 94-95 -0.8860730438825577 1101 96-97 -0.888993790488076 1101 98-99 -1.0168144625724764 1101 100 -0.9883793342115759 1102 1 -0.2943725777145154 1102 2 -0.2782077560717191 1102 3 -0.2797382279971714 1102 4 -0.18049700182575634 1102 5 -0.19974673037230417 1102 6 -0.2614814630651665 1102 7 -0.19865238921646977 1102 8 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68-69 -0.6649949543938689 1105 70-71 -0.6763762418694839 1105 72-73 -0.6004742645539167 1105 74-75 -0.5945742065178408 1105 76-77 -0.6154595611362836 1105 78-79 -0.5372376819412352 1105 80-81 -0.5429241347687963 1105 82-83 -0.5007687231713369 1105 84-85 -0.5093929731664169 1105 86-87 -0.44809492722697897 1105 88-89 -0.5279733217436053 1105 90-91 -0.5817800065800931 1105 92-93 -0.596659940308502 1105 94-95 -0.5732739037940817 1105 96-97 -0.569484652877005 1105 98-99 -0.610736833791119 1105 100 -0.7435902596287711 1106 1 -0.28766056904676773 1106 2 -0.26398699638961887 1106 3 -0.34296637814857434 1106 4 -0.20129772625542586 1106 5 -0.258359939406013 1106 6 -0.2984512770025347 1106 7 -0.305000717250536 1106 8 -0.3422795714046458 1106 9 -0.4201576724492355 1106 10-11 -0.41912917782462245 1106 12-13 -0.44351738648740024 1106 14-15 -0.5811938368297334 1106 16-17 -0.5686231437941558 1106 18-19 -0.5620304970485321 1106 20-21 -0.6311612260179018 1106 22-23 -0.5690737238676178 1106 24-25 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2208 52-53 1.0631617050951263 2208 54-55 1.032171278442469 2208 56-57 1.008714480388896 2208 58-59 0.977296654493081 2208 60-61 0.9795640461880026 2208 62-63 1.002442242665559 2208 64-65 0.9960885108444586 2208 66-67 1.0240096513010215 2208 68-69 1.018502748334548 2208 70-71 1.070218139667169 2208 72-73 1.0533369365008483 2208 74-75 1.0784968059884363 2208 76-77 1.0698484836354574 2208 78-79 0.9974637250937164 2208 80-81 0.9018623366116003 2208 82-83 0.9165332851588666 2208 84-85 0.946306125426883 2208 86-87 1.063955085902652 2208 88-89 0.997870026049565 2208 90-91 0.9982394402185797 2208 92-93 1.0249357656810751 2208 94-95 1.0666970067516672 2208 96-97 1.0563985178645723 2208 98-99 1.1202464413364979 2208 100 1.411232472400382 2209 1 0.30369236069635264 2209 2 0.388696785333039 2209 3 0.3884863955198057 2209 4 0.22709986979317875 2209 5 0.3392458598329213 2209 6 0.36757834814041246 2209 7 0.4462578573356879 2209 8 0.45108308701896505 2209 9 0.5989335554011603 2209 10-11 0.581638151992891 2209 12-13 0.617112388337496 2209 14-15 0.7829651837673524 2209 16-17 0.7666646646181192 2209 18-19 0.7627766373171667 2209 20-21 0.7490193802908252 2209 22-23 0.7580219686115441 2209 24-25 0.7779011329194887 2209 26-27 0.8287787440589796 2209 28-29 0.8512253689142852 2209 30-31 0.8709217452815281 2209 32-33 0.8849278591336969 2209 34-35 0.8848555692055342 2209 36-37 0.9073064932812969 2209 38-39 0.9422054188657469 2209 40-41 0.9594978578482056 2209 42-43 0.9752383682385002 2209 44-45 1.0020328918846957 2209 46-47 1.0017335124719438 2209 48-49 2.2473477726221134 2209 50-51 1.4162825256081035 2209 52-53 1.106749052017193 2209 54-55 1.0635925977126846 2209 56-57 1.0261908730806297 2209 58-59 1.06418658734583 2209 60-61 1.0181864484570369 2209 62-63 1.045732987477706 2209 64-65 1.089560180362625 2209 66-67 1.0449405639051932 2209 68-69 1.094466481327224 2209 70-71 1.1124280163293818 2209 72-73 1.0897640069833656 2209 74-75 1.0759298774634374 2209 76-77 1.0579579200113862 2209 78-79 0.9848955339725904 2209 80-81 0.9545029060429755 2209 82-83 0.9516796264771052 2209 84-85 0.9641936914781191 2209 86-87 0.9745690342030038 2209 88-89 0.9803430188970133 2209 90-91 1.0661593639171123 2209 92-93 1.0465006495588405 2209 94-95 1.050445240131051 2209 96-97 1.1422512335234458 2209 98-99 1.1748152141894472 2209 100 1.3582104772593482 2210 1 0.5602770474982393 2210 2 0.47764167604325536 2210 3 0.48249449901594943 2210 4 0.31416699850165486 2210 5 0.37479682312622487 2210 6 0.407175667960594 2210 7 0.4532900425942472 2210 8 0.4508784363114202 2210 9 0.5793028370168329 2210 10-11 0.6024047294985664 2210 12-13 0.6045250011996899 2210 14-15 0.7425444087240862 2210 16-17 0.7776110409070895 2210 18-19 0.7713351075111419 2210 20-21 0.763451164044973 2210 22-23 0.7994371368599715 2210 24-25 0.8095400721698098 2210 26-27 0.8336478507603999 2210 28-29 0.83675681376819 2210 30-31 0.8556993429032715 2210 32-33 0.8148541214246094 2210 34-35 0.8928174542813778 2210 36-37 0.9009074156921528 2210 38-39 0.9501675994398866 2210 40-41 0.9641240286694384 2210 42-43 0.8803137586643146 2210 44-45 0.9083430089372655 2210 46-47 0.9726334996773858 2210 48-49 2.2928683348444068 2210 50-51 1.4131900427319692 2210 52-53 1.1156223937793186 2210 54-55 1.0967819335217044 2210 56-57 1.1052642547402058 2210 58-59 1.0518990791263079 2210 60-61 1.0436647876032197 2210 62-63 1.0820258018095998 2210 64-65 1.1391065179154012 2210 66-67 1.1170081218638117 2210 68-69 1.0995079361078055 2210 70-71 1.1564485670428013 2210 72-73 1.1601296399728298 2210 74-75 1.1031252275438916 2210 76-77 1.0843887019745004 2210 78-79 1.0257668868757222 2210 80-81 1.0130535222035633 2210 82-83 1.0075138720533907 2210 84-85 1.0502880087246602 2210 86-87 1.0647568995710834 2210 88-89 1.102320947559793 2210 90-91 1.1602216991249712 2210 92-93 1.1560821037131603 2210 94-95 1.1579313522300403 2210 96-97 1.2024612229000233 2210 98-99 1.2170910859975201 2210 100 1.5172447619036191 2211 1 0.5054388582092955 2211 2 0.4689457321651176 2211 3 0.4652416498034597 2211 4 0.29824604349950334 2211 5 0.3464235332402765 2211 6 0.4097871386004286 2211 7 0.41259651316500623 2211 8 0.44074909813591034 2211 9 0.5684859369747173 2211 10-11 0.5927086179572001 2211 12-13 0.5505359918440362 2211 14-15 0.6850148172843333 2211 16-17 0.6858959645986715 2211 18-19 0.7146483583431333 2211 20-21 0.692842589249473 2211 22-23 0.7326543260882943 2211 24-25 0.7573486237617786 2211 26-27 0.8235626966057907 2211 28-29 0.8266462756113313 2211 30-31 0.80425632357111 2211 32-33 0.8022606901814058 2211 34-35 0.8312492996439929 2211 36-37 0.8362392593448931 2211 38-39 0.8451188299284311 2211 40-41 0.8435774136242173 2211 42-43 0.7747803145626833 2211 44-45 0.819278709599466 2211 46-47 0.9138671277015504 2211 48-49 2.166960078668559 2211 50-51 1.305515161812771 2211 52-53 1.0328177437236405 2211 54-55 1.011211450997095 2211 56-57 0.9707881498589828 2211 58-59 1.003211010472576 2211 60-61 0.9685107495850076 2211 62-63 1.0041685048252234 2211 64-65 1.0264205788801561 2211 66-67 1.0120314066888696 2211 68-69 1.0077745318980291 2211 70-71 1.024076655595671 2211 72-73 1.060273313990045 2211 74-75 1.0196391351854643 2211 76-77 0.8508288961978074 2211 78-79 0.7541411435757865 2211 80-81 0.797813206904749 2211 82-83 0.8897243219509967 2211 84-85 1.0005917002282807 2211 86-87 1.0167615604156701 2211 88-89 0.9950662125466003 2211 90-91 1.0193980156810163 2211 92-93 0.9898771968420981 2211 94-95 1.0175343781488486 2211 96-97 1.072538835596628 2211 98-99 1.165934518703267 2211 100 1.368539375269009 2212 1 0.5200080015882946 2212 2 0.4985710215549304 2212 3 0.468365073066515 2212 4 0.3027372580290546 2212 5 0.33954634648200255 2212 6 0.2391177614949882 2212 7 0.21635834466797377 2212 8 0.32222133825661814 2212 9 0.5265950349818027 2212 10-11 0.5325172905991948 2212 12-13 0.5860454307897953 2212 14-15 0.6803860236911703 2212 16-17 0.6668594180628986 2212 18-19 0.672155164380932 2212 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0.9735979309582383 2212 88-89 0.9967067924109649 2212 90-91 0.994961776049518 2212 92-93 1.0447281576595024 2212 94-95 1.0154465552954868 2212 96-97 1.0912853078304643 2212 98-99 1.1160249880408166 2212 100 1.3097250220500634 2213 1 0.38592379876633487 2213 2 0.3925167911085552 2213 3 0.42966139678073034 2213 4 0.2645122205010182 2213 5 0.32772849728138453 2213 6 0.35127594345497926 2213 7 0.40475075164147256 2213 8 0.3895880362188038 2213 9 0.5139347953316928 2213 10-11 0.4694534488816444 2213 12-13 0.5024432978286555 2213 14-15 0.6671214359089959 2213 16-17 0.6779281725752924 2213 18-19 0.6648407355022741 2213 20-21 0.5199030896031118 2213 22-23 0.6491041987749426 2213 24-25 0.6916393807599306 2213 26-27 0.7458857064501174 2213 28-29 0.7766226278105322 2213 30-31 0.7492458975636893 2213 32-33 0.7616981592658689 2213 34-35 0.7071525718827942 2213 36-37 0.7385713778837797 2213 38-39 0.689520843046715 2213 40-41 0.7660143563723594 2213 42-43 0.7703205753078493 2213 44-45 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2311 4 0.25442249670631156 2311 5 0.31904635514136714 2311 6 0.3686201576543837 2311 7 0.3871220016124326 2311 8 0.20589942409888096 2311 9 0.45958866655143993 2311 10-11 0.3729046260565312 2311 12-13 0.4662581406707673 2311 14-15 0.4531698381835483 2311 16-17 0.44354317833354173 2311 18-19 0.5945016406562189 2311 20-21 0.6474372961224262 2311 22-23 0.6808631717447824 2311 24-25 0.6173494705720941 2311 26-27 0.6904884221746599 2311 28-29 0.7294061904784073 2311 30-31 0.6273513537454107 2311 32-33 0.6644467188643901 2311 34-35 0.7926174804976043 2311 36-37 0.8149886691888497 2311 38-39 0.8426050385476884 2311 40-41 0.785964188759479 2311 42-43 0.811195830725211 2311 44-45 0.8249482263675745 2311 46-47 0.850809897147812 2311 48-49 -5.9209757144342205 2311 50-51 -1.021357392095048 2311 52-53 0.3551553080949077 2311 54-55 0.765189500798023 2311 56-57 0.8858923397883842 2311 58-59 0.9271676618320654 2311 60-61 0.8194723381117512 2311 62-63 0.6698625960413835 2311 64-65 0.7937981568848187 2311 66-67 0.9118154315840528 2311 68-69 0.905013041219263 2311 70-71 0.924986099104494 2311 72-73 0.47884627825930437 2311 74-75 0.7539893325443145 2311 76-77 0.8144555515844836 2311 78-79 0.7448678010904075 2311 80-81 0.7825121585303805 2311 82-83 0.745434573009522 2311 84-85 0.8564030247119412 2311 86-87 0.8964869581491577 2311 88-89 0.9690774566051239 2311 90-91 1.0019934588547095 2311 92-93 0.9769440095994852 2311 94-95 0.9561718652263203 2311 96-97 1.0584150059013133 2311 98-99 1.1064196021014787 2311 100 1.2822212508719382 2312 1 0.07584609528663577 2312 2 0.22132912761005485 2312 3 0.16702156593314754 2312 4 0.011363829184041663 2312 5 0.18058016005046795 2312 6 0.11377124154142848 2312 7 0.2577879516069004 2312 8 0.3131570536614632 2312 9 0.2516529172575801 2312 10-11 0.19338579521261323 2312 12-13 0.2568513295992787 2312 14-15 0.2796656913815738 2312 16-17 0.42039925490093566 2312 18-19 0.2858005677015072 2312 20-21 0.289374710240061 2312 22-23 0.5459480019690517 2312 24-25 0.6383797406062186 2312 26-27 0.5702943350669543 2312 28-29 0.7049599591971045 2312 30-31 0.71464908393272 2312 32-33 0.7178865248334816 2312 34-35 0.3652036475231526 2312 36-37 0.6684377478135701 2312 38-39 0.7555484210412402 2312 40-41 0.522877118181782 2312 42-43 0.5623304639600377 2312 44-45 0.6702344872436541 2312 46-47 0.7570634617105299 2312 48-49 -4.827199000401816 2312 50-51 -0.8836449580757275 2312 52-53 0.4867708069120482 2312 54-55 0.6943032576111605 2312 56-57 0.7987071892517719 2312 58-59 0.8597993502174788 2312 60-61 0.8953438088631316 2312 62-63 0.62386931815211 2312 64-65 0.8360793971864098 2312 66-67 0.856615690808745 2312 68-69 0.7451553863308575 2312 70-71 0.7619204021161821 2312 72-73 0.8363557401233166 2312 74-75 0.49170217623066037 2312 76-77 0.6763408061607699 2312 78-79 0.41698400387831924 2312 80-81 0.6380779604302091 2312 82-83 0.5492503298853713 2312 84-85 0.6713002904628915 2312 86-87 0.7874769384814648 2312 88-89 0.7932146650173912 2312 90-91 0.6938243385364018 2312 92-93 0.845304130342015 2312 94-95 0.9482697506459168 2312 96-97 1.0048690725372253 2312 98-99 1.0084627848928562 2312 100 0.5624889399134432 2313 1 0.11011908017938055 2313 2 0.23885022927509425 2313 3 0.28273026978571636 2313 4 0.08619943908831118 2313 5 0.14536284949179645 2313 6 0.24489660607489583 2313 7 0.11449347269482502 2313 8 0.20810619750880477 2313 9 0.40455348662413115 2313 10-11 0.2430895776161961 2313 12-13 -0.0019171536812692125 2313 14-15 0.11638601866480514 2313 16-17 0.11116759785418395 2313 18-19 0.4111635552737951 2313 20-21 0.37407331989637527 2313 22-23 0.4931679964434039 2313 24-25 0.2965056747768884 2313 26-27 0.3809536974272163 2313 28-29 0.3506323611694242 2313 30-31 0.5199601493645929 2313 32-33 0.4529029789376082 2313 34-35 0.39583772078108126 2313 36-37 -0.310331580761968 2313 38-39 0.1943570121114746 2313 40-41 0.40564354693746196 2313 42-43 0.47542492650752877 2313 44-45 0.5246337063243303 2313 46-47 0.41990777627935216 2313 48-49 -5.196995418599641 2313 50-51 -1.3812432584914873 2313 52-53 0.08947107676102206 2313 54-55 0.3499991716925521 2313 56-57 0.30080216751249367 2313 58-59 0.4547243115493913 2313 60-61 0.7145110652583782 2313 62-63 0.772260852960045 2313 64-65 0.9000483132330075 2313 66-67 0.9413836417200088 2313 68-69 0.9232281220905989 2313 70-71 0.8483259142723014 2313 72-73 0.5750786722059544 2313 74-75 0.7666368376348416 2313 76-77 0.747057992111305 2313 78-79 0.711498559920436 2313 80-81 -0.17703104722886565 2313 82-83 0.2793412334494967 2313 84-85 0.6490126671846319 2313 86-87 0.7745523573240938 2313 88-89 0.764035264273236 2313 90-91 0.7963476377602099 2313 92-93 0.883982996738979 2313 94-95 0.8148964687964657 2313 96-97 0.824906040659819 2313 98-99 1.002218371178838 2313 100 1.037923616203983 2314 1 0.09744535540404797 2314 2 0.196284493277048 2314 3 0.22038580319171075 2314 4 0.048842670014067835 2314 5 0.21397400084704543 2314 6 0.2555977160184142 2314 7 0.11030566766718408 2314 8 0.23735933558369027 2314 9 0.4485997178675589 2314 10-11 0.315746039184468 2314 12-13 0.22760635877821755 2314 14-15 0.28503673663040985 2314 16-17 0.49995691051894653 2314 18-19 0.5310504916597338 2314 20-21 0.5981044600973675 2314 22-23 0.32933682478065407 2314 24-25 0.441644544562557 2314 26-27 0.46925867443507485 2314 28-29 0.6376603916702024 2314 30-31 0.5377926224922049 2314 32-33 0.5276680958075985 2314 34-35 0.41630930261208476 2314 36-37 0.46562420223919787 2314 38-39 0.4916930182668864 2314 40-41 0.341884987926214 2314 42-43 0.38408028935988625 2314 44-45 0.5896778923504655 2314 46-47 0.7190958176776263 2314 48-49 -6.462873142236319 2314 50-51 -1.5322784192347214 2314 52-53 -0.7902281395684412 2314 54-55 -0.2961701365178513 2314 56-57 -0.01894983893797786 2314 58-59 0.45185163401823303 2314 60-61 0.6822756378646133 2314 62-63 0.5600203486918218 2314 64-65 0.7044669667884449 2314 66-67 0.7901306837758497 2314 68-69 0.8025798939636033 2314 70-71 0.5464376796817803 2314 72-73 0.7392407984168656 2314 74-75 0.7404231304609752 2314 76-77 0.7190999053115199 2314 78-79 0.4985457140281824 2314 80-81 0.5683743406359838 2314 82-83 0.6065594495969897 2314 84-85 0.5985224986747859 2314 86-87 0.60079845338797 2314 88-89 0.5183462520233313 2314 90-91 0.7103120990277212 2314 92-93 0.7781624106090881 2314 94-95 0.8905957590936318 2314 96-97 0.8736604998631634 2314 98-99 0.9292386065428637 2314 100 -0.4120895879487243 2315 1 0.1993865059582518 2315 2 0.2501630906290444 2315 3 0.32995960869757823 2315 4 0.12815752486327625 2315 5 0.2343073872013406 2315 6 0.30014413962545916 2315 7 0.3601467229596622 2315 8 0.2521341856645378 2315 9 0.4108968001674427 2315 10-11 0.23609205552414636 2315 12-13 0.40659390515500604 2315 14-15 0.23519345606676723 2315 16-17 0.3541776766287654 2315 18-19 0.4642869682845543 2315 20-21 0.3693812533051073 2315 22-23 0.4213962576749495 2315 24-25 0.4558576808838737 2315 26-27 0.4938311772144033 2315 28-29 0.6202900230189599 2315 30-31 0.6077564705105587 2315 32-33 0.619677453814603 2315 34-35 0.6464162207312043 2315 36-37 0.5635091612014236 2315 38-39 0.6752553324221751 2315 40-41 0.7075239321730891 2315 42-43 0.7395499254216986 2315 44-45 0.7082504530622771 2315 46-47 0.5886927147092251 2315 48-49 -4.938247446675639 2315 50-51 -0.968532363559298 2315 52-53 0.2687385918481695 2315 54-55 0.22238380986352269 2315 56-57 0.5329268516709291 2315 58-59 0.641520549876681 2315 60-61 0.5432152368642065 2315 62-63 0.32099640442206834 2315 64-65 0.484469915712765 2315 66-67 -0.29181387081462873 2315 68-69 0.5072143518123582 2315 70-71 0.4750227537093892 2315 72-73 0.6242442358950697 2315 74-75 0.7433550195120233 2315 76-77 0.6685859355620316 2315 78-79 0.5836558790316602 2315 80-81 0.4203961415547326 2315 82-83 0.4693311809791574 2315 84-85 0.49997283235734 2315 86-87 0.5346196784719126 2315 88-89 0.5044818446095221 2315 90-91 0.5640808283304999 2315 92-93 0.6996217414275527 2315 94-95 0.27300477680764246 2315 96-97 0.38489921050936715 2315 98-99 0.37066824998100145 2315 100 0.4007611946200562 2316 1 0.19466367680865915 2316 2 0.2282998384908268 2316 3 0.1826787324050514 2316 4 -0.061220823242344125 2316 5 0.13474754661651644 2316 6 0.13147495552040311 2316 7 0.24438140218423854 2316 8 0.31237677562238275 2316 9 0.4399821871588472 2316 10-11 0.37725965170792364 2316 12-13 0.30195614282073535 2316 14-15 0.41846862411231456 2316 16-17 0.4163266318641945 2316 18-19 0.1404465764038747 2316 20-21 0.43698515002994753 2316 22-23 0.5583482559103317 2316 24-25 0.591846218685049 2316 26-27 0.6424242278282648 2316 28-29 0.6998722522299019 2316 30-31 0.6026814456547598 2316 32-33 0.583576687983836 2316 34-35 0.5218166122762611 2316 36-37 0.46358637942675784 2316 38-39 0.5028863260194782 2316 40-41 0.14907482949005413 2316 42-43 0.10335053712864806 2316 44-45 0.3644469090636093 2316 46-47 0.3648460797580242 2316 48-49 -5.174093617659956 2316 50-51 -1.0283601380134186 2316 52-53 0.25158393651437194 2316 54-55 0.5362152577300634 2316 56-57 0.43473567105157684 2316 58-59 0.3241248008848103 2316 60-61 0.4707840312052127 2316 62-63 0.35412394888552257 2316 64-65 0.41239218402374433 2316 66-67 0.48502286362815283 2316 68-69 0.17583101112611388 2316 70-71 -0.22832006388733106 2316 72-73 -0.056652509355927805 2316 74-75 0.12551807437506568 2316 76-77 0.20034608067517112 2316 78-79 0.2435609034786239 2316 80-81 0.19123421606168733 2316 82-83 0.12245134519637091 2316 84-85 0.25094113859621814 2316 86-87 0.3709109545168019 2316 88-89 0.18503890449916938 2316 90-91 0.1759179638506012 2316 92-93 0.316522692413443 2316 94-95 0.5451593621520878 2316 96-97 0.6069515892105066 2316 98-99 0.5060215209415162 2316 100 -0.300460723689703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 12.0 11 20.0 12 43.0 13 232.0 14 844.0 15 2582.0 16 6171.0 17 12393.0 18 21663.0 19 33699.0 20 49927.0 21 71210.0 22 90621.0 23 97490.0 24 101383.0 25 110669.0 26 130817.0 27 160346.0 28 197736.0 29 242530.0 30 299570.0 31 371968.0 32 468937.0 33 612559.0 34 847162.0 35 1200575.0 36 1876480.0 37 2718785.0 38 2110516.0 39 272607.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30528821471942 19.871531015680958 14.344714754892983 23.478466014706644 2 15.495808068041402 20.216984700974034 39.957460910522485 24.329746320462075 3 18.870568470648934 25.268430457553333 29.772656498447798 26.088344573349936 4 13.0198574605565 16.72817263587618 37.719469674768085 32.532500228799236 5 13.7723927347007 37.64053006942974 34.193040708177406 14.39403648769215 6 31.5859757426384 36.76788943876661 17.326317609696996 14.319817208897994 7 28.193664712069 32.28729546751792 21.396551883601465 18.12248793681162 8 26.622849917103448 35.160360813895835 19.393623026974495 18.823166242026225 9 26.4552769279026 16.05561269719302 20.278826597988786 37.210283776915595 10-11 25.470645448829398 26.163217042183806 27.155257628060898 21.210879880925894 12-13 25.88951728590953 23.602349036725904 27.868235008878727 22.639898668485834 14-15 23.28579124975061 25.103356424746348 25.402397215024962 26.208455110478074 16-17 22.576785629494136 28.14739636773532 26.221073906697413 23.054744096073133 18-19 22.31333384229733 27.238299570839303 28.491387367169587 21.956979219693785 20-21 23.309850923161086 26.2087835475119 28.39697196277272 22.084393566554294 22-23 23.303282219477552 25.968322729498812 28.198817221256522 22.529577829767113 24-25 22.94146085930671 26.232322392766477 28.37769681973287 22.44851992819395 26-27 22.808244065019704 26.404072484879716 28.141645939241393 22.646037510859188 28-29 22.55682210203223 26.50049793803404 28.374713658275734 22.567966301658 30-31 22.99994590905206 26.2773247030056 28.253407420039334 22.469321967903007 32-33 22.953700423521767 26.537813717896586 27.90313180973742 22.605354048844227 34-35 23.067388380557237 26.447018275945506 28.176960177864434 22.308633165632827 36-37 23.00749506095294 26.313609757525903 28.062819367515363 22.616075814005793 38-39 23.023074888012864 26.278216078984578 28.24008581502343 22.458623217979127 40-41 22.884577793417186 26.030924238003543 28.41458672147763 22.66991124710164 42-43 22.837133994071017 26.124554886530184 28.12757479132654 22.910736328072257 44-45 22.757565914739278 26.502593264573278 27.97937611177222 22.76046470891523 46-47 22.870328919040894 26.374445140441367 27.689784340383156 23.065441600134587 48-49 23.250364551356288 26.44586145920013 27.505595220730672 22.798178768712905 50-51 23.004466849078284 26.507010248925123 27.40075586731306 23.087767034683537 52-53 22.81780907369005 26.733197782259438 27.289156788211805 23.159836355838706 54-55 22.775576712122017 26.647466501051646 27.311658164650538 23.2652986221758 56-57 23.030953828162936 26.532788875418294 27.127696825748142 23.308560470670624 58-59 22.741276699978865 26.588557846227513 27.43692343039153 23.23324202340209 60-61 22.842530663433138 26.760346278918757 27.261730913958225 23.135392143689877 62-63 22.857673290988252 26.610941657462185 27.139343313579054 23.392041737970505 64-65 22.526223048100846 26.550298558949088 27.275595954338232 23.647882438611838 66-67 22.742405905038094 26.44143898379659 27.182567258066175 23.63358785309914 68-69 22.52031201011965 26.553926545315083 27.21001282550771 23.715748619057557 70-71 22.595748879477163 26.34235895793183 27.24345248447491 23.8184396781161 72-73 22.776130371341484 26.5909061154817 27.055304439704475 23.577659073472333 74-75 22.719533163252876 26.76109542914345 26.96246411681424 23.556907290789432 76-77 22.63275098702094 26.647213597711715 26.850975272288935 23.869060142978405 78-79 22.548924269165337 26.626419850691274 26.94828371275783 23.876372167385558 80-81 22.415567825257945 26.945202922249855 26.864062347804644 23.775166904687552 82-83 22.73017412624784 26.58442653743202 27.169669325848787 23.51573001047135 84-85 22.80758385904261 26.67939142683601 26.72890169110923 23.784123023012153 86-87 22.363179973273393 26.90994127489234 26.96033501886544 23.766543732968827 88-89 22.488515004282736 26.53514987348393 26.926072999246546 24.050262122986783 90-91 22.520698026924315 26.48779593543791 26.958382752011094 24.033123285626683 92-93 22.574298328606872 26.634946863382964 26.805294413929225 23.985460394080935 94-95 22.21483134034159 26.46609926496214 27.034489234409058 24.284580160287213 96-97 22.29072059149426 26.425270819448322 26.968356971354297 24.315651617703125 98-99 22.083897492492436 26.573028944869886 26.90856968964298 24.434503872994696 100 22.13255955638624 26.47943742836195 26.74467219462831 24.643330820623497 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1760.0 1 1266.0 2 1077.0 3 1372.0 4 1144.5 5 1075.0 6 1779.0 7 3573.0 8 6074.0 9 7574.0 10 7079.0 11 6302.5 12 6699.5 13 7781.5 14 8982.0 15 10182.0 16 11340.5 17 12690.0 18 14078.0 19 15124.5 20 16377.5 21 18055.0 22 20231.0 23 23239.5 24 27121.0 25 32317.5 26 39763.0 27 48800.5 28 59572.0 29 76040.5 30 97893.0 31 120449.0 32 149326.5 33 181636.5 34 211262.0 35 239886.0 36 274684.0 37 317676.0 38 343014.0 39 346855.0 40 344273.5 41 344263.0 42 355446.0 43 376506.0 44 402222.0 45 437951.5 46 471899.5 47 495274.0 48 514753.5 49 522377.0 50 513970.5 51 503759.5 52 492239.5 53 477435.0 54 461879.5 55 428512.0 56 385463.0 57 336387.5 58 291324.0 59 257810.5 60 217768.0 61 175819.5 62 140034.5 63 108698.5 64 79589.0 65 55829.5 66 39779.5 67 29525.0 68 22235.0 69 15857.0 70 11792.5 71 8761.5 72 6121.5 73 4473.0 74 3298.5 75 2253.5 76 1501.5 77 883.5 78 484.5 79 295.0 80 190.5 81 115.0 82 76.5 83 56.0 84 31.0 85 22.5 86 16.0 87 12.0 88 10.0 89 6.0 90 4.0 91 2.0 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 1.0 98 1.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029835906642432387 2 0.0019571297742198937 3 7.43213838311352E-5 4 0.023832390415184024 5 5.119917552811536E-4 6 7.43213838311352E-5 7 1.6515863073585602E-5 8 0.004145481631469986 9 0.010371962010211757 10-11 0.001123078689003821 12-13 8.670828113632441E-5 14-15 1.486427676622704E-4 16-17 3.71606919155676E-4 18-19 2.8902760378774802E-5 20-21 7.845034959953161E-5 22-23 2.9315656955614447E-4 24-25 4.541862345236041E-5 26-27 8.257931536792802E-5 28-29 0.0010859179970882534 30-31 0.0027664070648255883 32-33 0.010070547509118821 34-35 0.019583684639504127 36-37 0.022548282061212743 38-39 0.02146236406412449 40-41 0.00459966786599359 42-43 0.004781342359803032 44-45 0.008976371580493775 46-47 0.011879034515676444 48-49 1.2075986182828953 50-51 0.017787584530251695 52-53 0.01818809420978614 54-55 0.009641135069205596 56-57 0.007349559067745593 58-59 0.008200126016035252 60-61 0.0049795327166860595 62-63 0.00729588251275644 64-65 0.01923685151495883 66-67 0.02946842868904511 68-69 0.02986480940281117 70-71 0.02708188647491199 72-73 0.02336581728335523 74-75 0.009769133008025883 76-77 0.014843631937385061 78-79 0.008542830174812152 80-81 0.0027953098252043633 82-83 0.014653699512038825 84-85 0.02468708632924208 86-87 0.024018193874761864 88-89 0.032973920626413654 90-91 0.02112791783688438 92-93 0.013844422221433133 94-95 0.006655892818654998 96-97 0.003645876773494022 98-99 0.016098837530977565 100 0.036706505681044 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.210957E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.001374673802225 #Duplication Level Percentage of deduplicated Percentage of total 1 69.71757218260186 34.16256875867525 2 15.195337196792815 14.891848225496165 3 6.13029664861901 9.011788888216026 4 3.004586896781779 5.889155530768027 5 1.616805573528568 3.9612847841582517 6 1.0471789169438634 3.07879238758036 7 0.6930231673266105 2.3771361515857463 8 0.484151297580038 1.8979263305221568 9 0.3345120108479689 1.4752393538803485 >10 1.6413297786686427 13.839084350452605 >50 0.08838237919358273 2.9369036908046215 >100 0.043668123935403064 3.8841990745915953 >500 0.0023531028093472417 0.8075086467451886 >1k 6.920037703565368E-4 0.5524476385875959 >5k 2.7680150008233905E-5 0.10209362377696567 >10k+ 8.304045002470171E-5 1.1320225641591013 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36270 0.29951517683947493 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29688 0.2451614714643047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17502 0.1445303177569476 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.257931536792801E-6 2 0.0 0.0 0.0 0.0 8.257931536792801E-6 3 0.0 0.0 0.0 0.0 8.257931536792801E-6 4 0.0 0.0 0.0 8.257931536792801E-6 8.257931536792801E-6 5 0.0 8.257931536792801E-6 0.0 8.257931536792801E-6 8.257931536792801E-6 6 0.0 8.257931536792801E-6 0.0 8.257931536792801E-6 8.257931536792801E-6 7 0.0 8.257931536792801E-6 0.0 2.4773794610378403E-5 8.257931536792801E-6 8 0.0 8.257931536792801E-6 0.0 2.4773794610378403E-5 8.257931536792801E-6 9 0.0 8.257931536792801E-6 0.0 6.606345229434241E-5 8.257931536792801E-6 10-11 0.0 8.257931536792801E-6 0.0 7.432138383113522E-5 8.257931536792801E-6 12-13 0.0 1.2386897305189202E-5 0.0 8.257931536792802E-5 2.0644828841982E-5 14-15 0.0 2.8902760378774805E-5 0.0 8.257931536792802E-5 3.71606919155676E-5 16-17 0.0 4.128965768396401E-5 0.0 1.0322414420991E-4 7.43213838311352E-5 18-19 0.0 4.128965768396401E-5 0.0 1.23868973051892E-4 9.909517844151361E-5 20-21 0.0 4.128965768396401E-5 0.0 1.7754552804104522E-4 1.0322414420991E-4 22-23 0.0 4.128965768396401E-5 0.0 4.1289657683964E-4 1.4038483612547763E-4 24-25 0.0 5.780552075754961E-5 0.0 0.001164368346687785 1.7341656227264882E-4 26-27 0.0 6.606345229434241E-5 0.0 0.002332865659143966 1.7341656227264882E-4 28-29 0.0 6.606345229434241E-5 0.0 0.0064122838383196094 1.7341656227264882E-4 30-31 0.0 6.606345229434241E-5 0.0 0.018774407348898434 1.7754552804104522E-4 32-33 0.0 6.606345229434241E-5 0.0 0.045546621391180694 2.2296415149340563E-4 34-35 0.0 6.606345229434241E-5 0.0 0.08636144801177911 2.559958776405768E-4 36-37 0.0 7.019241806273881E-5 0.0 0.15146698024785354 2.6838277494576604E-4 38-39 0.0 8.2579315367928E-5 0.0 0.24084670223633042 2.7664070648255885E-4 40-41 0.0 9.496621267311722E-5 0.0 0.33635793839087597 2.89027603787748E-4 42-43 0.0 9.909517844151361E-5 0.0 0.43355792154469563 3.0141450109293723E-4 44-45 0.0 9.909517844151361E-5 0.0 0.5527363894836893 3.1380139839812645E-4 46-47 0.0 9.909517844151361E-5 0.0 0.6945746215596424 3.30317261471712E-4 48-49 0.0 9.909517844151361E-5 0.0 0.8471192618730475 3.4683312454529764E-4 50-51 0.0 1.1148207574670282E-4 0.0 1.0102175386904737 3.4683312454529764E-4 52-53 0.0 1.23868973051892E-4 0.0 1.1714330071175112 3.7160691915567607E-4 54-55 0.0 1.3212690458868482E-4 0.0 1.3490735013712296 3.9638071376605445E-4 56-57 0.0 1.4038483612547763E-4 0.0 1.5583460023766327 4.0050967953445086E-4 58-59 0.0 1.6515863073585603E-4 0.0 1.7922684290193622 4.3354140568162205E-4 60-61 0.0 2.023193226514236E-4 0.0 2.0323017249993187 4.3767037145001845E-4 62-63 0.0 2.1470621995661282E-4 0.0 2.2725703720280737 4.541862345236041E-4 64-65 0.0 2.1470621995661282E-4 0.0 2.5194412353204942 4.541862345236041E-4 66-67 0.0 2.2296415149340563E-4 0.0 2.7979523632961367 4.541862345236041E-4 68-69 0.0 2.2296415149340563E-4 0.0 3.1058906303031404 4.541862345236041E-4 70-71 4.1289657683964005E-6 2.3535104879859482E-4 0.0 3.4350104917020174 4.665731318287933E-4 72-73 8.257931536792801E-6 2.47737946103784E-4 0.0 3.7637876489421176 6.028290021858745E-4 74-75 8.257931536792801E-6 2.47737946103784E-4 0.0 4.089658014281267 6.028290021858745E-4 76-77 8.257931536792801E-6 2.6425380917736963E-4 0.0 4.448845004405607 6.234738310278565E-4 78-79 8.257931536792801E-6 2.6425380917736963E-4 0.0 4.838499632934943 6.441186598698385E-4 80-81 8.257931536792801E-6 2.6425380917736963E-4 0.0 5.25142924150073 6.977952148589917E-4 82-83 8.257931536792801E-6 2.6425380917736963E-4 0.0 5.6691897400155415 8.010193590689017E-4 84-85 8.257931536792801E-6 2.6425380917736963E-4 0.0 6.078448698013224 8.010193590689017E-4 86-87 8.257931536792801E-6 2.6425380917736963E-4 0.0 6.502753607270943 8.010193590689017E-4 88 8.257931536792801E-6 2.6425380917736963E-4 0.0 6.842183496193506 8.010193590689017E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20655 0.0 50.119663 1 GTATCAA 37675 0.0 41.876686 1 CAACGCA 46955 0.0 32.96804 5 ATCAACG 47345 0.0 32.703625 3 TATCAAC 48505 0.0 32.37402 2 TCAACGC 49770 0.0 31.214258 4 AACGCAG 50205 0.0 31.12313 6 GTGGTAT 9050 0.0 27.534285 1 ACGCAGA 56925 0.0 27.144146 7 CGCAGAG 58575 0.0 26.315456 8 GTACTAG 4440 0.0 25.36624 1 GTACATG 52640 0.0 24.872313 1 TACATGG 53820 0.0 23.929083 2 GCAGAGT 63840 0.0 23.829018 9 ACATGGG 54430 0.0 22.929754 3 TGGTATC 10595 0.0 22.716625 2 TAGTACT 7005 0.0 21.500984 4 GAGTACT 33530 0.0 21.500006 12-13 CTAGTAC 6940 0.0 21.026018 3 CATGGGG 38205 0.0 20.325407 4 >>END_MODULE