FastQCFastQC Report
Mon 6 Jun 2016
ERR1211329_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211329_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3439244
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156140.45399512218382876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126600.3681041531220233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76680.22295597520850513No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52970.1540164059310709No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60700.053.8660431
TACCTGG38200.045.5650672
GTATCAA113400.042.523581
GTACCTG45100.038.7004781
ACCTGGG44700.038.0596473
ATCAACG142700.033.1641773
CAACGCA143350.033.0137985
TATCAAC144650.032.8145452
TCAACGC147300.032.1608734
AACGCAG151300.031.6499966
GTACATG133700.028.7833181
CCTGGGG43300.028.5455154
ACGCAGA169750.028.0996577
CGCAGAG173150.027.4912058
TACATGG138100.027.4558582
GTGGTAT29750.027.3576071
TGGTATC29250.026.0545042
ACATGGG140500.025.8563293
GCAGAGT186150.024.9413349
CATGGGG98950.024.0803174
Produced by FastQC (version 0.11.5)