##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211329_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3439244 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20795267797225 31.0 31.0 34.0 28.0 34.0 2 31.199212094285837 31.0 31.0 34.0 28.0 34.0 3 31.24073662700291 31.0 31.0 34.0 27.0 34.0 4 34.86387124612269 37.0 35.0 37.0 32.0 37.0 5 34.913845891713414 37.0 35.0 37.0 32.0 37.0 6 34.978994802346094 37.0 35.0 37.0 32.0 37.0 7 34.914492545454756 37.0 35.0 37.0 32.0 37.0 8 34.91373743764618 37.0 35.0 37.0 32.0 37.0 9 36.440622997379656 39.0 37.0 39.0 32.0 39.0 10-11 36.3640728892745 39.0 37.0 39.0 32.0 39.0 12-13 36.27188169841977 39.0 35.0 39.0 31.0 39.0 14-15 37.35429326910216 40.0 37.0 41.0 31.0 41.0 16-17 37.343848677209294 40.0 37.0 41.0 31.0 41.0 18-19 37.357003748498215 40.0 37.0 41.0 31.0 41.0 20-21 37.32714646009414 40.0 37.0 41.0 31.0 41.0 22-23 37.19266414363157 40.0 36.5 41.0 31.0 41.0 24-25 37.07189966748507 40.0 36.0 41.0 30.0 41.0 26-27 36.9235183662456 39.5 36.0 41.0 30.0 41.0 28-29 36.77040593804918 39.0 36.0 41.0 30.0 41.0 30-31 36.56224565631284 39.0 35.5 41.0 30.0 41.0 32-33 36.40179891278432 39.0 35.0 41.0 29.0 41.0 34-35 36.237356669081926 39.0 35.0 40.0 29.0 41.0 36-37 36.12362033632973 39.0 35.0 40.0 28.0 41.0 38-39 35.961943089818575 39.0 35.0 40.0 27.0 41.0 40-41 35.786055016742054 38.0 35.0 40.0 27.0 41.0 42-43 35.62605386532621 38.0 34.5 40.0 26.5 41.0 44-45 35.48394211053359 38.0 34.0 40.0 26.0 41.0 46-47 35.295367237683635 38.0 34.0 40.0 24.5 41.0 48-49 35.036990251345934 38.0 33.5 40.0 24.0 41.0 50-51 34.37107573641184 37.5 32.5 39.5 23.5 40.5 52-53 34.61825389533281 38.0 33.0 40.0 24.0 40.5 54-55 34.973862133654954 38.0 34.0 40.0 24.0 41.0 56-57 34.885366958552524 38.0 34.0 40.0 24.0 41.0 58-59 34.67084801194681 38.0 33.0 40.0 24.0 41.0 60-61 34.40728849130797 37.0 33.0 40.0 23.0 41.0 62-63 34.131206887327565 37.0 33.0 40.0 23.0 41.0 64-65 33.85181001987647 36.0 32.0 39.5 22.5 41.0 66-67 33.62565275973441 36.0 32.0 39.0 22.0 41.0 68-69 33.28298108537807 35.5 32.0 39.0 22.0 41.0 70-71 32.92716335334161 35.0 31.5 38.5 21.5 40.0 72-73 32.55560233586218 35.0 31.0 37.5 20.5 40.0 74-75 32.183229657447974 35.0 31.0 37.0 20.0 39.0 76-77 31.82298130054163 35.0 31.0 36.5 20.0 39.0 78-79 31.455562181688766 35.0 30.0 36.0 19.0 39.0 80-81 31.123417384750834 34.0 30.0 35.5 18.0 37.5 82-83 30.808610700491155 34.0 29.5 35.0 18.0 37.0 84-85 30.50513732087633 34.0 29.0 35.0 18.0 36.5 86-87 30.24270784509619 34.0 29.0 35.0 17.0 36.0 88-89 29.975519474628726 34.0 29.0 35.0 15.5 36.0 90-91 29.71439842593314 34.0 29.0 35.0 15.0 35.5 92-93 29.470942160544585 34.0 27.5 35.0 12.5 35.0 94-95 29.231748750597518 34.0 27.0 35.0 11.0 35.0 96-97 29.021756961704376 34.0 27.0 35.0 10.0 35.0 98-99 28.774623289304277 33.0 26.0 35.0 10.0 35.0 100 27.0953854975105 31.0 23.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4080792094876635 1101 2 -0.47220962018884194 1101 3 -0.3812657018282408 1101 4 -0.2918542557098647 1101 5 -0.3661185918623602 1101 6 -0.35238225930967815 1101 7 -0.4272657249767917 1101 8 -0.49071725731003113 1101 9 -0.4757045094359853 1101 10-11 -0.5401624535180218 1101 12-13 -0.514532403607582 1101 14-15 -0.6270630321297617 1101 16-17 -0.5723389610148288 1101 18-19 -0.7089070719843917 1101 20-21 -0.6306331253931319 1101 22-23 -0.7118932579554809 1101 24-25 -0.6021592011265469 1101 26-27 -0.6896157467032751 1101 28-29 -0.7921026295462141 1101 30-31 -0.7333662028350929 1101 32-33 -0.8082838434705693 1101 34-35 -0.8910421922424732 1101 36-37 -0.8826291838201143 1101 38-39 -0.8689379993203801 1101 40-41 -0.9237383788538835 1101 42-43 -0.85031698482058 1101 44-45 -0.9093499151279048 1101 46-47 -0.7434613095213578 1101 48-49 -0.8863498322053758 1101 50-51 -0.8996947103739785 1101 52-53 -0.6955308910350055 1101 54-55 -0.5863885328794609 1101 56-57 -0.6740853276002099 1101 58-59 -0.7194583430656323 1101 60-61 -0.6024135840830525 1101 62-63 -0.7484110216733981 1101 64-65 -0.7462672628959837 1101 66-67 -0.7097070300112094 1101 68-69 -0.7197920740398658 1101 70-71 -0.847209117808319 1101 72-73 -0.844836507087237 1101 74-75 -0.7832652513488441 1101 76-77 -0.7385770201072006 1101 78-79 -0.8329133224874532 1101 80-81 -0.9921213981014212 1101 82-83 -1.208535736720659 1101 84-85 -1.1138188472939525 1101 86-87 -0.9412783217914793 1101 88-89 -0.8811275614185767 1101 90-91 -0.9775219868594611 1101 92-93 -1.0484960224699833 1101 94-95 -1.0242324497611115 1101 96-97 -0.9902063188597374 1101 98-99 -1.1918175802969877 1101 100 -1.22875872178275 1102 1 -0.41465175087033046 1102 2 -0.3957130826119304 1102 3 -0.3745106671792655 1102 4 -0.23993399351343925 1102 5 -0.38521635752616135 1102 6 -0.3838555429141124 1102 7 -0.43317314542106544 1102 8 -0.49862694850668277 1102 9 -0.406163542893907 1102 10-11 -0.533445103213019 1102 12-13 -0.5302235251967673 1102 14-15 -0.6394667488886725 1102 16-17 -0.706300514529488 1102 18-19 -0.6142581766563424 1102 20-21 -0.7783020368300768 1102 22-23 -0.8906431440260718 1102 24-25 -0.87571036955773 1102 26-27 -0.7422727867985941 1102 28-29 -0.7980512398548356 1102 30-31 -0.7595888870986371 1102 32-33 -0.8519314384567735 1102 34-35 -0.7144412417103183 1102 36-37 -0.7721771449063652 1102 38-39 -0.9727756368141058 1102 40-41 -0.9283015353399051 1102 42-43 -0.9578676385588878 1102 44-45 -0.925806168089558 1102 46-47 -0.9398177232008109 1102 48-49 -0.8249759548482771 1102 50-51 -0.8623065313186018 1102 52-53 -0.7371574504245046 1102 54-55 -0.6686168024679944 1102 56-57 -0.5925818243481231 1102 58-59 -0.5200693449557221 1102 60-61 -0.6056642840529918 1102 62-63 -0.7374296698045271 1102 64-65 -0.830137147052973 1102 66-67 -0.7304748668205789 1102 68-69 -0.6906121224820296 1102 70-71 -0.7849751507181395 1102 72-73 -0.812846880953046 1102 74-75 -0.6912940065138145 1102 76-77 -0.7147870123249476 1102 78-79 -0.9020915192230312 1102 80-81 -0.9043366209679711 1102 82-83 -0.8723597356780743 1102 84-85 -0.9302227855192591 1102 86-87 -1.0439779539592884 1102 88-89 -1.1619685625621976 1102 90-91 -1.0586408108027427 1102 92-93 -1.015523613389778 1102 94-95 -1.145604339281956 1102 96-97 -0.9732040811004978 1102 98-99 -1.0928312736843786 1102 100 -1.0890755919551829 1103 1 -0.4272547162739535 1103 2 -0.48251875300960023 1103 3 -0.5026563583845807 1103 4 -0.2676661484445759 1103 5 -0.3620245189800997 1103 6 -0.4664416989212228 1103 7 -0.3955293078546802 1103 8 -0.40808731035877344 1103 9 -0.7126274152513474 1103 10-11 -0.5795031113309292 1103 12-13 -0.710019384182047 1103 14-15 -0.7856326678895655 1103 16-17 -0.7867002453279426 1103 18-19 -0.74175478023718 1103 20-21 -0.8440013433116391 1103 22-23 -0.7593327646947259 1103 24-25 -0.8641725400422757 1103 26-27 -0.9137045580233334 1103 28-29 -0.9421215316014866 1103 30-31 -0.9038092702189076 1103 32-33 -0.6756765950269923 1103 34-35 -0.8083734786997852 1103 36-37 -1.0217023788877242 1103 38-39 -1.0618710903224127 1103 40-41 -0.8400639262515242 1103 42-43 -1.0130935710826563 1103 44-45 -1.0991800212093423 1103 46-47 -1.1386641443484038 1103 48-49 -1.0890542430948855 1103 50-51 -1.0611253205815174 1103 52-53 -0.8182638876069674 1103 54-55 -0.8404847178725632 1103 56-57 -0.7238473541999682 1103 58-59 -0.7430543106984686 1103 60-61 -0.8956726887512474 1103 62-63 -0.8871687368934857 1103 64-65 -0.8204390881928703 1103 66-67 -0.9838256515033024 1103 68-69 -0.9011955529805178 1103 70-71 -0.917525458541526 1103 72-73 -0.9261458767852346 1103 74-75 -0.8922975463257075 1103 76-77 -0.8389851120048775 1103 78-79 -0.8745639344381502 1103 80-81 -0.7977715886993728 1103 82-83 -0.9496627590667899 1103 84-85 -0.9689132853842395 1103 86-87 -1.0654946618295646 1103 88-89 -1.1535855825471693 1103 90-91 -0.9878632558477385 1103 92-93 -1.0349066478312459 1103 94-95 -1.2296536768493915 1103 96-97 -1.3158280948829493 1103 98-99 -1.4036521918584164 1103 100 -1.3054620322357735 1104 1 -0.39117459350992334 1104 2 -0.3624210560895733 1104 3 -0.41457881132363994 1104 4 -0.3406098718408259 1104 5 -0.24434091650809364 1104 6 -0.24527912020756304 1104 7 -0.32522369399271867 1104 8 -0.2665567113405487 1104 9 -0.4448453423121208 1104 10-11 -0.41689136512131597 1104 12-13 -0.5222086063698939 1104 14-15 -0.6353677802420705 1104 16-17 -0.5395120371141218 1104 18-19 -0.5640391591833165 1104 20-21 -0.6383807912080144 1104 22-23 -0.5773306468479475 1104 24-25 -0.6888436779510982 1104 26-27 -0.7094149252282804 1104 28-29 -0.6667383121398771 1104 30-31 -0.6994407147033073 1104 32-33 -0.8039965236933924 1104 34-35 -0.7562510847175616 1104 36-37 -0.7347680091405309 1104 38-39 -0.8403730639130984 1104 40-41 -0.7230693034498685 1104 42-43 -0.7912591561770341 1104 44-45 -0.8077612729341439 1104 46-47 -0.7961415755863328 1104 48-49 -0.7369466928561295 1104 50-51 -0.7759232261502262 1104 52-53 -0.5903518482856782 1104 54-55 -0.6037107546348679 1104 56-57 -0.5057762463967634 1104 58-59 -0.6499263889898046 1104 60-61 -0.5913547796702261 1104 62-63 -0.6751814209768057 1104 64-65 -0.5746756180183397 1104 66-67 -0.643010583640212 1104 68-69 -0.5123618112700186 1104 70-71 -0.6183646527144191 1104 72-73 -0.7028255717985736 1104 74-75 -0.6079119623184823 1104 76-77 -0.5696922700342952 1104 78-79 -0.6525510810107065 1104 80-81 -0.6438985215220256 1104 82-83 -0.6704077268286923 1104 84-85 -0.7148104537833753 1104 86-87 -0.7298924657917958 1104 88-89 -0.6115153298272098 1104 90-91 -0.5999777889179256 1104 92-93 -0.7166522407029028 1104 94-95 -0.6522657680192445 1104 96-97 -0.8267344831283765 1104 98-99 -0.899258809659198 1104 100 -0.9385743471071137 1105 1 -0.4210377489781827 1105 2 -0.5349799735954583 1105 3 -0.5094665310937003 1105 4 -0.3438127758551559 1105 5 -0.31718529697383246 1105 6 -0.3837580704658521 1105 7 -0.30892973397754275 1105 8 -0.509572713754558 1105 9 -0.5655987156014604 1105 10-11 -0.491488323277963 1105 12-13 -0.43884465770440073 1105 14-15 -0.6269581687086188 1105 16-17 -0.7350377063692832 1105 18-19 -0.7809502382517124 1105 20-21 -0.7774560400926518 1105 22-23 -0.7971077612268189 1105 24-25 -0.6853455260766452 1105 26-27 -0.7299688186180902 1105 28-29 -0.7987480226028723 1105 30-31 -0.817378077734709 1105 32-33 -0.835525763536701 1105 34-35 -0.7944669732345275 1105 36-37 -0.9600506306773937 1105 38-39 -0.8264766045662526 1105 40-41 -0.8822186054202916 1105 42-43 -1.0162129130184567 1105 44-45 -0.9844798736093594 1105 46-47 -0.8086330813324096 1105 48-49 -0.827832358397437 1105 50-51 -0.8304203683190252 1105 52-53 -0.6702370047720478 1105 54-55 -0.6616056984227185 1105 56-57 -0.6929005582521413 1105 58-59 -0.5911128851871084 1105 60-61 -0.6957477096495595 1105 62-63 -0.6629855632912793 1105 64-65 -0.7646263453425846 1105 66-67 -0.7401082101315595 1105 68-69 -0.6888433932462661 1105 70-71 -0.5855037959973686 1105 72-73 -0.6071069859307698 1105 74-75 -0.6628042023522909 1105 76-77 -0.6731370251676694 1105 78-79 -0.8248931899388623 1105 80-81 -0.5689360803314791 1105 82-83 -0.8087589131027784 1105 84-85 -0.7287459326712025 1105 86-87 -0.6809661634996083 1105 88-89 -0.8009107290431352 1105 90-91 -0.8347970363970596 1105 92-93 -0.9377501165191156 1105 94-95 -0.8092693454108009 1105 96-97 -0.8787575362733904 1105 98-99 -1.052171438303624 1105 100 -0.9909410723631211 1106 1 -0.40454829144582405 1106 2 -0.44740284319357926 1106 3 -0.45621019281221464 1106 4 -0.2612587375454396 1106 5 -0.25849201666711963 1106 6 -0.30550189276915773 1106 7 -0.29189581755320404 1106 8 -0.36450928888177714 1106 9 -0.42945187565004517 1106 10-11 -0.4848075373851941 1106 12-13 -0.4401383593244077 1106 14-15 -0.6199143082626009 1106 16-17 -0.662851633566568 1106 18-19 -0.6495773545680166 1106 20-21 -0.6397429631994598 1106 22-23 -0.6037335506539847 1106 24-25 -0.6475103741588413 1106 26-27 -0.6239603782817582 1106 28-29 -0.7723406957767409 1106 30-31 -0.6674281343064408 1106 32-33 -0.7349735353998668 1106 34-35 -0.7158836613889719 1106 36-37 -0.9514729561224939 1106 38-39 -0.8024159567397007 1106 40-41 -0.8039125699711249 1106 42-43 -0.741633117520891 1106 44-45 -0.8024843950651714 1106 46-47 -0.6321630958523343 1106 48-49 -0.7503520346217982 1106 50-51 -0.7372992393682125 1106 52-53 -0.673321303219943 1106 54-55 -0.7293678897902325 1106 56-57 -0.5755364795087061 1106 58-59 -0.6063110881057057 1106 60-61 -0.6212394279098277 1106 62-63 -0.6498816918520944 1106 64-65 -0.7703093644344321 1106 66-67 -0.7581024235735114 1106 68-69 -0.6032757137943037 1106 70-71 -0.7398002342879977 1106 72-73 -0.6472143360952245 1106 74-75 -0.625145540760716 1106 76-77 -0.6595917749750413 1106 78-79 -0.5827276628124167 1106 80-81 -0.8284433878568471 1106 82-83 -0.7724890804994153 1106 84-85 -0.6887809676832717 1106 86-87 -0.7580387548578251 1106 88-89 -0.793278975344105 1106 90-91 -0.7821957311027496 1106 92-93 -0.8751939309614052 1106 94-95 -0.9338499514791927 1106 96-97 -1.091331263807195 1106 98-99 -0.8553775364879144 1106 100 -1.0969074547794264 1107 1 -0.5571329538778507 1107 2 -0.4921224987825994 1107 3 -0.5296368243765777 1107 4 -0.26824040120993686 1107 5 -0.37341394807177153 1107 6 -0.2517246757207161 1107 7 -0.3402836153697635 1107 8 -0.3605883322710497 1107 9 -0.4562936648952274 1107 10-11 -0.4756032463472266 1107 12-13 -0.4024374144306506 1107 14-15 -0.6013215606321367 1107 16-17 -0.5808900856618919 1107 18-19 -0.6149806785909746 1107 20-21 -0.5790822911507618 1107 22-23 -0.709906645855618 1107 24-25 -0.7283288121062839 1107 26-27 -0.6826086964583808 1107 28-29 -0.7115469072316642 1107 30-31 -0.6138440771703912 1107 32-33 -0.7244065748364719 1107 34-35 -0.7108230953278465 1107 36-37 -0.6210713945074957 1107 38-39 -0.7045857848016084 1107 40-41 -0.8105659638826737 1107 42-43 -0.7707970554938512 1107 44-45 -0.7483836728425501 1107 46-47 -0.7768479894636684 1107 48-49 -0.7473911892493916 1107 50-51 -0.7088034120209912 1107 52-53 -0.6014106342098842 1107 54-55 -0.6117864483106885 1107 56-57 -0.6394480528163697 1107 58-59 -0.5680967355414666 1107 60-61 -0.590225958251196 1107 62-63 -0.6284787664979703 1107 64-65 -0.6181064031028782 1107 66-67 -0.7154618259592098 1107 68-69 -0.4407954588407179 1107 70-71 -0.4922561072390508 1107 72-73 -0.5945139138465905 1107 74-75 -0.4771078422467312 1107 76-77 -0.4252052752631066 1107 78-79 -0.47027883949321136 1107 80-81 -0.5317082425250383 1107 82-83 -0.48756022100830165 1107 84-85 -0.46778658064875955 1107 86-87 -0.5129215486512315 1107 88-89 -0.5120392125034421 1107 90-91 -0.5362397159436121 1107 92-93 -0.5207841917734761 1107 94-95 -0.5175921391501141 1107 96-97 -0.5670029843306175 1107 98-99 -0.5571657120675049 1107 100 -0.6187563058109795 1108 1 -0.5466707751108935 1108 2 -0.5439220498234434 1108 3 -0.666450044908732 1108 4 -0.5896325898742916 1108 5 -0.525261440383403 1108 6 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0.8269609419319508 2311 22-23 0.870267897645995 2311 24-25 0.8857018001386123 2311 26-27 0.8729364163235687 2311 28-29 0.8855919219842363 2311 30-31 0.8600025930766577 2311 32-33 0.9540722114148608 2311 34-35 0.936851448173762 2311 36-37 0.8546912263303099 2311 38-39 0.8913191561221296 2311 40-41 0.9120441327875781 2311 42-43 0.8851011722182847 2311 44-45 0.8837497858040706 2311 46-47 1.0092570051657646 2311 48-49 1.0279243316958215 2311 50-51 0.9442175331992075 2311 52-53 0.8854529533304003 2311 54-55 0.7742876655453799 2311 56-57 0.7311319686659132 2311 58-59 0.8704370990449988 2311 60-61 0.8505823124254945 2311 62-63 0.8922516309241573 2311 64-65 0.8045426301946534 2311 66-67 0.8092374221610967 2311 68-69 0.8205055615674439 2311 70-71 0.8268013578522471 2311 72-73 0.7910875899128129 2311 74-75 0.8453894724686322 2311 76-77 0.8472933820945769 2311 78-79 0.8459778737183896 2311 80-81 0.8545592118251228 2311 82-83 0.8808742580464788 2311 84-85 0.9024497243995739 2311 86-87 0.9793663138621973 2311 88-89 0.934336995874645 2311 90-91 1.1139939038200843 2311 92-93 1.0499650026411338 2311 94-95 1.1119500198187353 2311 96-97 1.0369309576672343 2311 98-99 1.155663235300917 2311 100 1.3232522870998693 2312 1 0.521133785289269 2312 2 0.5862841162587458 2312 3 0.6476770338350342 2312 4 0.5350798695417609 2312 5 0.5121623367800083 2312 6 0.5219715348805991 2312 7 0.4893486948321737 2312 8 0.5895528561113821 2312 9 0.7105835030870722 2312 10-11 0.7305389170412937 2312 12-13 0.7713180465409764 2312 14-15 0.943700095753222 2312 16-17 0.9005757984998866 2312 18-19 0.8346368190183853 2312 20-21 0.8169213088717413 2312 22-23 0.8565248318411207 2312 24-25 0.8974994743205116 2312 26-27 0.997228365023247 2312 28-29 0.9650021336998407 2312 30-31 0.9917220826181108 2312 32-33 0.9227764075571585 2312 34-35 0.8851630670856778 2312 36-37 0.9642018884505248 2312 38-39 1.0376338540711387 2312 40-41 0.9985625306704193 2312 42-43 0.9005017651237708 2312 44-45 0.897935304151062 2312 46-47 0.8564130309177074 2312 48-49 0.9737533179037783 2312 50-51 0.9715783403649567 2312 52-53 0.7967792294767619 2312 54-55 0.8744187154715917 2312 56-57 0.8206250726406665 2312 58-59 0.7813829487065576 2312 60-61 0.858629159593292 2312 62-63 0.7640033740154095 2312 64-65 0.8625751636569134 2312 66-67 0.8644476109747714 2312 68-69 0.770633112587852 2312 70-71 0.8206184732270785 2312 72-73 0.9696679247899098 2312 74-75 0.8709263954672508 2312 76-77 0.9195581920085658 2312 78-79 0.9531678070483309 2312 80-81 1.013591478098217 2312 82-83 1.0376436865161196 2312 84-85 1.0565898842041435 2312 86-87 1.0596884676613705 2312 88-89 1.0464257154023535 2312 90-91 1.0760498608662452 2312 92-93 1.098796368105024 2312 94-95 1.1270102646149063 2312 96-97 1.1769463528527382 2312 98-99 1.2640284828962116 2312 100 1.2405686899702424 2313 1 0.44637693360049013 2313 2 0.4662475360968834 2313 3 0.43186098542983586 2313 4 0.35848278658150434 2313 5 0.407024834344206 2313 6 0.4115465587452718 2313 7 0.4571567957470464 2313 8 0.4783084646173137 2313 9 0.5633698113026853 2313 10-11 0.6130765763549348 2313 12-13 0.6388955173052224 2313 14-15 0.7758568453234105 2313 16-17 0.723637888576377 2313 18-19 0.7386193823923435 2313 20-21 0.7131953986226378 2313 22-23 0.6713405373843884 2313 24-25 0.6907717954958059 2313 26-27 0.6880505183441983 2313 28-29 0.7916446027004369 2313 30-31 0.8549009307500057 2313 32-33 0.9096537986491668 2313 34-35 0.7921720036956756 2313 36-37 0.8265235200786734 2313 38-39 0.915471591577024 2313 40-41 1.043955354249455 2313 42-43 0.8832762211696163 2313 44-45 0.9768550917246586 2313 46-47 0.9608392181696601 2313 48-49 1.0103070251292792 2313 50-51 0.9419120264267633 2313 52-53 0.8034710804625504 2313 54-55 0.7550442411726621 2313 56-57 0.7893184480054174 2313 58-59 0.8794592835864776 2313 60-61 0.811792924266669 2313 62-63 0.7863714153364327 2313 64-65 0.7806693939565363 2313 66-67 0.8654653435967319 2313 68-69 0.9459545764849864 2313 70-71 0.8663623602947723 2313 72-73 0.8014768791596723 2313 74-75 0.7967686888436063 2313 76-77 0.8521893983645867 2313 78-79 0.8151506466851401 2313 80-81 0.8291696197865228 2313 82-83 0.8767282821487008 2313 84-85 0.8391753345249313 2313 86-87 0.8336013609967772 2313 88-89 0.9081115724708617 2313 90-91 0.965866923057451 2313 92-93 0.9959835435886681 2313 94-95 1.0632758691495674 2313 96-97 1.090486994325218 2313 98-99 1.0675176580789305 2313 100 1.1414147449613523 2314 1 0.35316121300893855 2314 2 0.2703681910791147 2314 3 0.3478085704067695 2314 4 0.18588556189178007 2314 5 0.22557129335936565 2314 6 0.25931854341073546 2314 7 0.36998517883246507 2314 8 0.40822216502613173 2314 9 0.45424105885130217 2314 10-11 0.5419123883605224 2314 12-13 0.4840728369523859 2314 14-15 0.611116784116426 2314 16-17 0.6534996491898397 2314 18-19 0.5433102739263873 2314 20-21 0.5983926058889679 2314 22-23 0.704268165244379 2314 24-25 0.708184229527987 2314 26-27 0.7559814081946357 2314 28-29 0.6105412423853238 2314 30-31 0.7040314255398741 2314 32-33 0.7813702125054576 2314 34-35 0.7515746605660993 2314 36-37 0.802708726254572 2314 38-39 0.80627071071725 2314 40-41 0.8441776492322575 2314 42-43 0.7183473046643343 2314 44-45 0.9069061970573387 2314 46-47 0.6874269730539524 2314 48-49 0.6905238606216884 2314 50-51 0.8373767340393599 2314 52-53 0.805256243275764 2314 54-55 0.8088060894414113 2314 56-57 0.6737093954802802 2314 58-59 0.7282850526747424 2314 60-61 0.519680884560934 2314 62-63 0.7597000551231261 2314 64-65 0.7982632196179509 2314 66-67 0.8077830502094443 2314 68-69 0.9044777439584593 2314 70-71 0.8761284489063854 2314 72-73 0.8440511157486768 2314 74-75 0.8669854191105202 2314 76-77 0.8623859938547653 2314 78-79 0.8022631537878624 2314 80-81 0.8606556583205922 2314 82-83 0.926882119380025 2314 84-85 0.9200879919777591 2314 86-87 0.9404807348903823 2314 88-89 0.8787018410210159 2314 90-91 0.8578006422502433 2314 92-93 0.9688315720298952 2314 94-95 0.9983120324614951 2314 96-97 1.04188266373313 2314 98-99 1.0843411352090264 2314 100 1.219660914875572 2315 1 0.35750482224636215 2315 2 0.34012710602706164 2315 3 0.40272724680581007 2315 4 0.260930309910556 2315 5 0.20070206574415295 2315 6 0.15643842035033373 2315 7 0.19746429946903277 2315 8 0.23719556617521675 2315 9 0.13128851063221703 2315 10-11 0.2528626006928292 2315 12-13 0.27827007808202353 2315 14-15 0.3553531394119247 2315 16-17 0.18830772422471398 2315 18-19 0.46694403718665 2315 20-21 0.2889698924840971 2315 22-23 0.2513894673964998 2315 24-25 0.4260180965242313 2315 26-27 0.5674966259317884 2315 28-29 0.465371348525899 2315 30-31 0.4903836635255132 2315 32-33 0.48023486628478196 2315 34-35 0.43664520614962754 2315 36-37 0.543001749948921 2315 38-39 0.5759860287796101 2315 40-41 0.682067947335554 2315 42-43 0.702400821029066 2315 44-45 0.6000950942376377 2315 46-47 0.5789314517538529 2315 48-49 0.5470061778759501 2315 50-51 0.589323367261045 2315 52-53 0.277759447567675 2315 54-55 0.4169722282922024 2315 56-57 0.4240122226877574 2315 58-59 0.3540816794768773 2315 60-61 0.5584575674019305 2315 62-63 0.6323218687681802 2315 64-65 0.6800962305121772 2315 66-67 0.5004644742817916 2315 68-69 0.46142582553714107 2315 70-71 0.4220564096514039 2315 72-73 0.505867430948193 2315 74-75 0.34124327927938936 2315 76-77 0.5002859639131145 2315 78-79 0.723772314980863 2315 80-81 0.6573579236288971 2315 82-83 0.5110226467963663 2315 84-85 0.5885744844956378 2315 86-87 0.6642124236502802 2315 88-89 0.6306293495960453 2315 90-91 0.8063974900699442 2315 92-93 0.9134464735160392 2315 94-95 0.9001184223348382 2315 96-97 0.9135121883869921 2315 98-99 0.9673196149872076 2315 100 1.1233351858428229 2316 1 0.015217068504039588 2316 2 0.04526557280909316 2316 3 0.031862382773486075 2316 4 -0.07681471287203578 2316 5 -0.12864835093058957 2316 6 -0.23154766912754354 2316 7 -0.19065022311010438 2316 8 -0.1494798597780047 2316 9 -0.13590786184066417 2316 10-11 -0.11720241328576009 2316 12-13 0.01976560826231122 2316 14-15 -0.08316764911266716 2316 16-17 0.02549193902646607 2316 18-19 0.061127793902066685 2316 20-21 0.13680399368342222 2316 22-23 -0.03419960580341552 2316 24-25 -0.0404990586105356 2316 26-27 -0.13647867665610391 2316 28-29 -0.03839861015193691 2316 30-31 -0.6745091606662115 2316 32-33 -0.5177907542557634 2316 34-35 -0.5516167039710425 2316 36-37 -0.32379834305950084 2316 38-39 -0.21977018879848487 2316 40-41 -0.0475076495802611 2316 42-43 0.17812808278069525 2316 44-45 -0.03256400257707526 2316 46-47 -0.1818933222709589 2316 48-49 -0.0984321367240355 2316 50-51 0.09156740654914586 2316 52-53 -0.23194135050617604 2316 54-55 -0.20264606455614143 2316 56-57 -0.20656238253572212 2316 58-59 -0.5335956240341417 2316 60-61 -0.1110563642358855 2316 62-63 -0.1774846692467733 2316 64-65 -0.579555194101566 2316 66-67 -0.8472170970835364 2316 68-69 -0.7919299550206276 2316 70-71 -0.49161412640401636 2316 72-73 -0.7404245911452847 2316 74-75 -0.5179031200476913 2316 76-77 -0.2459222164556465 2316 78-79 -0.07262681336330346 2316 80-81 -0.176213147572728 2316 82-83 0.011913045912784526 2316 84-85 0.17645042457873927 2316 86-87 0.132102160976018 2316 88-89 0.043084988007926484 2316 90-91 -0.4545811800984474 2316 92-93 -0.40876690081652 2316 94-95 -0.036838438551495045 2316 96-97 0.19319936311215358 2316 98-99 0.2887638191267783 2316 100 0.13935640786018055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 101.0 1 254.0 2 146.0 3 130.0 4 167.0 5 133.0 6 165.0 7 501.0 8 1423.0 9 2209.0 10 3468.0 11 5202.0 12 7509.0 13 9601.0 14 11242.0 15 13139.0 16 15774.0 17 18859.0 18 23054.0 19 27354.0 20 31798.0 21 37746.0 22 42261.0 23 43426.0 24 43081.0 25 44334.0 26 47641.0 27 53496.0 28 62020.0 29 73000.0 30 87829.0 31 106743.0 32 133010.0 33 170449.0 34 230218.0 35 316565.0 36 449437.0 37 633439.0 38 567032.0 39 125162.0 40 126.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.76738588538239 18.26307938530837 12.854760940167933 23.114773789141307 2 16.35213260526652 19.954276053523916 40.40552494775716 23.288066393452407 3 19.038687028015467 26.45044365964535 29.653802398135092 24.857066914204086 4 12.876835265717482 16.457579105416016 37.458366909774156 33.207218719092346 5 13.181571781040866 36.856932306092375 34.15911610436353 15.80237980850324 6 30.103008933685473 35.911664406895035 19.162116821805977 14.823209837613518 7 27.302295517643415 32.51968783380303 22.436858138219765 17.741158510333786 8 26.242053779063422 34.62971446688511 20.63241083725645 18.495820916795015 9 27.318260311111224 16.22166318650938 20.816808767029574 35.643267735349816 10-11 25.207690606114948 25.857707701877246 28.27390392603345 20.660697765974355 12-13 25.52566466972338 24.012330408526225 28.24395663240115 22.218048289349245 14-15 23.1815154947676 25.162860374251284 26.477842917887834 25.17778121309328 16-17 22.26670639991867 27.718684503580864 27.237813257496924 22.77679583900354 18-19 21.878856303821003 26.853959988173216 29.452449760519215 21.814733947486566 20-21 22.864875054326102 26.327113249052964 29.202251355170826 21.605760341450104 22-23 22.788998196988462 26.048644075981315 29.093996885019763 22.06836084201046 24-25 22.28144722573557 26.01847075250815 29.42797462708324 22.27210739467304 26-27 22.12988900666926 26.173135398305913 29.21621842968461 22.480757165340222 28-29 22.090103802342814 26.531220194990674 29.343569419916786 22.035106582749727 30-31 22.815293064908197 26.296555202977018 29.280149246610648 21.608002485504134 32-33 22.628868738371533 26.38011521710547 29.03702027295121 21.95399577157179 34-35 22.635656637161446 26.519134272475554 29.007788918009314 21.83742017235368 36-37 22.557790609631336 26.49299740909809 28.818118802175462 22.131093179095114 38-39 22.49725354123368 26.73521432123524 28.784691450466216 21.982840687064858 40-41 22.56403982841738 26.238368577379678 29.153597400513686 22.04399419368925 42-43 22.384165860075925 26.51513234052123 28.627250926379283 22.473450873023562 44-45 22.08750292390175 26.82052887418436 28.847549217558072 22.244418984355814 46-47 22.19760205521499 26.43351536610699 28.466022326896724 22.90286025178129 48-49 22.338256949852077 26.614513833660908 28.417567330294663 22.62966188619235 50-51 22.316022791225457 26.901381591856925 28.292122871068347 22.490472745849267 52-53 22.315175510491468 27.062645056154572 27.9892296578064 22.63294977554756 54-55 22.20131066448717 26.88801111740446 28.251810258231796 22.658867959876574 56-57 22.42539315699617 26.875729762195167 27.907982796558557 22.79089428425011 58-59 22.14879182642796 26.926517562590547 28.24199545007593 22.682695160905563 60-61 22.52735104994054 27.0267180369149 28.084963962475364 22.3609669506692 62-63 22.523863276011046 26.947231685426598 28.03580727840828 22.493097760154075 64-65 22.262999877812494 26.70045733037756 28.065029935938835 22.971512855871108 66-67 22.246138745378435 26.651697101808068 27.973560977000478 23.128603175813016 68-69 21.879994030074805 26.703268824975 28.191371596131653 23.225365548818537 70-71 22.073010453858792 26.479835973406047 28.2370268652461 23.21012670748906 72-73 22.10244199123437 26.939234536972158 27.730785853322253 23.227537618471217 74-75 22.144894768658613 26.915229031550492 27.731002474491444 23.20887372529945 76-77 22.068711016427848 26.713524409474477 27.80333711959721 23.41442745450047 78-79 21.928346859436182 26.694574255075125 27.835273655550765 23.54180522993793 80-81 21.966252562872757 26.859982991282376 27.663154654514422 23.51060979133044 82-83 22.316129875914186 26.706807243049617 27.841335629823476 23.13572725121272 84-85 22.27758073380269 26.877965175633246 27.44723816628171 23.397215924282357 86-87 21.944643448092776 26.96058273117052 27.752070143028064 23.34270367770864 88-89 21.95260863727174 26.53138594343022 27.80481623892395 23.711189180374088 90-91 22.343180153038688 26.44450703536859 27.752389010870065 23.459923800722652 92-93 22.264587636538014 26.5266498372172 27.694943992443772 23.51381853380102 94-95 21.974391438748704 26.354405796350772 27.752467840164446 23.918734924736075 96-97 22.010569847926586 26.325497484841758 27.72474748393245 23.9391851832992 98-99 21.9381732324572 26.378992069950773 27.502943158304593 24.179891539287436 100 22.073683950035008 26.359429429036002 27.378381014351618 24.188505606577372 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1846.0 1 1357.5 2 2485.0 3 3482.5 4 2096.5 5 1425.5 6 1881.5 7 3294.0 8 5220.5 9 6071.0 10 5168.0 11 4021.0 12 3831.0 13 4120.5 14 4497.5 15 4850.0 16 5104.5 17 5460.0 18 5873.0 19 6113.5 20 6531.5 21 7216.0 22 8057.5 23 9173.0 24 10628.0 25 12467.5 26 15204.5 27 18475.0 28 22039.0 29 26798.5 30 33314.0 31 39560.0 32 46494.0 33 54675.5 34 63152.0 35 71246.0 36 80419.5 37 90370.5 38 95739.5 39 98546.5 40 99716.0 41 100501.0 42 102667.0 43 106483.5 44 111803.0 45 118477.0 46 124022.5 47 128009.0 48 133427.0 49 137955.5 50 139594.0 51 137550.5 52 133011.0 53 128188.0 54 122252.0 55 113573.5 56 104282.5 57 93553.0 58 81811.5 59 71097.0 60 59071.5 61 47324.5 62 38030.0 63 29999.5 64 22592.5 65 16638.5 66 12361.0 67 9409.5 68 7209.0 69 5310.5 70 4034.0 71 3095.0 72 2356.5 73 1807.5 74 1390.5 75 1010.0 76 690.5 77 463.0 78 310.5 79 208.0 80 154.0 81 106.5 82 64.5 83 56.0 84 45.5 85 31.5 86 26.5 87 18.0 88 11.5 89 12.0 90 12.5 91 7.5 92 5.5 93 5.5 94 4.5 95 4.0 96 3.5 97 2.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02433674377275936 2 0.11179782533603316 3 0.016631562052590625 4 0.016660638210025225 5 0.01884135001762015 6 0.045969404904101015 7 0.04617293800614321 8 0.03747916693319811 9 0.034338941930261414 10-11 0.036563267974008246 12-13 0.036039897140185455 14-15 0.031969235099341596 16-17 0.03830783742008418 18-19 0.03737740038217702 20-21 0.04855718291578033 22-23 0.048324573656303535 24-25 0.06835804612874224 26-27 0.05976604160681825 28-29 0.0522643929886917 30-31 0.04977838152803349 32-33 0.045358805597974436 34-35 0.046623618446379494 36-37 0.042015047492995554 38-39 0.047554055484286664 40-41 0.05668396891875075 42-43 0.056320516950818265 44-45 0.059780579685535545 46-47 0.06204851996543427 48-49 0.06113262100624439 50-51 0.05432880016654823 52-53 0.06434553640276759 54-55 0.050912351667982844 56-57 0.05549184646393219 58-59 0.05303491116070857 60-61 0.04989468615777188 62-63 0.056814811627206446 64-65 0.05536100375547649 66-67 0.05306398731814317 68-69 0.058588457230716984 70-71 0.0580505483181769 72-73 0.060173107810902626 74-75 0.05146479865924023 76-77 0.052874992294818284 78-79 0.06356048015203342 80-81 0.06347325167972961 82-83 0.05304944923942588 84-85 0.05969335121323174 86-87 0.05761440595665792 88-89 0.06593018698295323 90-91 0.07170180423372113 92-93 0.07587423282558609 94-95 0.07958144289849746 96-97 0.07506010041741731 98-99 0.08387017612010081 100 0.12906906285218495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3439244.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.5651802345149 #Duplication Level Percentage of deduplicated Percentage of total 1 80.43313554375541 52.7361302875349 2 12.47758466101256 16.361901743814137 3 3.428315203065125 6.743343125690774 4 1.3005959319409202 3.410952267599332 5 0.655007794404531 2.1472852047572575 6 0.38606922101430985 1.5187618835281194 7 0.27048077884334115 1.2413884710384937 8 0.17804459911259915 0.933882098447961 9 0.1377148168423794 0.8126367108510433 >10 0.6722096401969392 7.748164436062304 >50 0.03879970926808846 1.7519304306149983 >100 0.020531983369467176 2.3841245339105424 >500 0.001006744783606971 0.4318864388331977 >1k 3.0202343436511127E-4 0.4165727205264469 >5k 1.0067447812170375E-4 0.42789299014620374 >10k+ 1.0067447812170375E-4 0.9331466566443322 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15614 0.45399512218382876 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12660 0.3681041531220233 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7668 0.22295597520850513 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5297 0.1540164059310709 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.9076157434599E-5 2 0.0 0.0 0.0 0.0 5.8152314869198E-5 3 0.0 0.0 0.0 0.0 5.8152314869198E-5 4 0.0 0.0 0.0 0.0 5.8152314869198E-5 5 0.0 0.0 0.0 0.0 5.8152314869198E-5 6 0.0 0.0 0.0 0.0 5.8152314869198E-5 7 0.0 0.0 0.0 0.0 5.8152314869198E-5 8 0.0 0.0 0.0 0.0 8.7228472303797E-5 9 0.0 0.0 0.0 0.0 1.16304629738396E-4 10-11 0.0 0.0 0.0 2.9076157434599E-5 1.16304629738396E-4 12-13 0.0 0.0 0.0 4.36142361518985E-5 1.308427084556955E-4 14-15 0.0 0.0 0.0 7.26903935864975E-5 1.599188658902945E-4 16-17 0.0 0.0 0.0 8.7228472303797E-5 2.03533102042193E-4 18-19 0.0 0.0 0.0 1.16304629738396E-4 2.32609259476792E-4 20-21 0.0 0.0 0.0 2.03533102042193E-4 2.32609259476792E-4 22-23 0.0 1.45380787172995E-5 0.0 4.65218518953584E-4 2.9076157434599E-4 24-25 0.0 2.9076157434599E-5 0.0 0.001337503241991554 3.48913889215188E-4 26-27 0.0 2.9076157434599E-5 0.0 0.002645930326548509 4.506804402362845E-4 28-29 0.0 2.9076157434599E-5 0.0 0.006425830793046379 4.94294676388183E-4 30-31 0.0 2.9076157434599E-5 0.0 0.01917572582811804 5.233708338227821E-4 32-33 0.0 2.9076157434599E-5 0.0 0.049589386504708594 5.8152314869198E-4 34-35 0.0 2.9076157434599E-5 0.0 0.09467196860705435 5.8152314869198E-4 36-37 0.0 2.9076157434599E-5 0.0 0.1629718624209274 6.10599306126579E-4 38-39 0.0 2.9076157434599E-5 0.0 0.2703355737481842 6.832896997130764E-4 40-41 0.0 2.9076157434599E-5 0.0 0.3968459347461244 7.26903935864975E-4 42-43 0.0 2.9076157434599E-5 0.0 0.517061307659474 7.850562507341729E-4 44-45 0.0 2.9076157434599E-5 0.0 0.6582115139257348 8.14132408168772E-4 46-47 0.0 2.9076157434599E-5 0.0 0.8216049806294639 8.286704868860715E-4 48-49 0.0 2.9076157434599E-5 0.0 0.9968760576452267 8.7228472303797E-4 50-51 0.0 2.9076157434599E-5 0.0 1.184737110830171 8.7228472303797E-4 52-53 0.0 2.9076157434599E-5 0.0 1.3809430211988447 9.158989591898684E-4 54-55 0.0 2.9076157434599E-5 0.0 1.6033320113373755 0.001046741667645564 56-57 0.0 2.9076157434599E-5 0.0 1.8433847671174246 0.0011194320612320616 58-59 0.0 2.9076157434599E-5 0.0 2.1186487495507738 0.00116304629738396 60-61 0.0 2.9076157434599E-5 0.0 2.393534741937472 0.00116304629738396 62-63 0.0 2.9076157434599E-5 0.0 2.6752390932425847 0.001192122454818559 64-65 0.0 2.9076157434599E-5 0.0 2.956260154847984 0.001192122454818559 66-67 0.0 5.8152314869198E-5 0.0 3.2822329558472734 0.001192122454818559 68-69 0.0 5.8152314869198E-5 0.0 3.63847403673598 0.001192122454818559 70-71 0.0 5.8152314869198E-5 0.0 4.011739207802645 0.001192122454818559 72-73 0.0 5.8152314869198E-5 0.0 4.3632117988720776 0.001308427084556955 74-75 0.0 5.8152314869198E-5 0.0 4.7178391530231645 0.0013665793994261529 76-77 0.0 5.8152314869198E-5 0.0 5.123364320763517 0.001395655556860752 78-79 0.0 5.8152314869198E-5 0.0 5.553502455772257 0.001395655556860752 80-81 0.0 5.8152314869198E-5 0.0 5.990095497731478 0.0014683459504472494 82-83 0.0 5.8152314869198E-5 0.0 6.4275172101775855 0.001628264816337544 84-85 1.45380787172995E-5 5.8152314869198E-5 0.0 6.883445896830815 0.001628264816337544 86-87 2.9076157434599E-5 5.8152314869198E-5 0.0 7.355947993221767 0.0016428028950548435 88 2.9076157434599E-5 5.8152314869198E-5 0.0 7.720330398192161 0.001657340973772143 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6070 0.0 53.866043 1 TACCTGG 3820 0.0 45.565067 2 GTATCAA 11340 0.0 42.52358 1 GTACCTG 4510 0.0 38.700478 1 ACCTGGG 4470 0.0 38.059647 3 ATCAACG 14270 0.0 33.164177 3 CAACGCA 14335 0.0 33.013798 5 TATCAAC 14465 0.0 32.814545 2 TCAACGC 14730 0.0 32.160873 4 AACGCAG 15130 0.0 31.649996 6 GTACATG 13370 0.0 28.783318 1 CCTGGGG 4330 0.0 28.545515 4 ACGCAGA 16975 0.0 28.099657 7 CGCAGAG 17315 0.0 27.491205 8 TACATGG 13810 0.0 27.455858 2 GTGGTAT 2975 0.0 27.357607 1 TGGTATC 2925 0.0 26.054504 2 ACATGGG 14050 0.0 25.856329 3 GCAGAGT 18615 0.0 24.941334 9 CATGGGG 9895 0.0 24.080317 4 >>END_MODULE