FastQCFastQC Report
Mon 6 Jun 2016
ERR1211329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211329_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3439244
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141140.41038088603193024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120460.35025139245717957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77780.22615435252631103No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43340.12601606632155207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69850.051.6097681
TACCTGG36650.048.6506652
ACCTGGG42500.041.1863
GTACCTG47350.038.3643721
GTATCAA118200.037.408521
CCTGGGG40600.030.5145934
CAACGCA147600.029.5596965
ATCAACG147700.029.5165043
TCAACGC150500.029.0297224
TATCAAC151900.029.0059432
AACGCAG155050.028.4112856
CTGGGGG26150.026.7311695
GTGGTAT34200.026.6264171
GTACATG137450.026.398051
TATCACG3450.025.8412592
TGGGGGG58800.025.531116
GTACACG6350.025.1328831
TACATGG142150.025.1198462
TAACGCA4150.024.877364
ACGCAGA177150.024.6550337
Produced by FastQC (version 0.11.5)