##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211329_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3439244 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06727670383375 33.0 31.0 34.0 30.0 34.0 2 32.18492058138359 33.0 31.0 34.0 30.0 34.0 3 32.245786864787725 34.0 31.0 34.0 30.0 34.0 4 35.90859880834277 37.0 35.0 37.0 35.0 37.0 5 35.81616861147392 37.0 35.0 37.0 35.0 37.0 6 35.83439471000022 37.0 35.0 37.0 35.0 37.0 7 35.75031082412298 37.0 35.0 37.0 33.0 37.0 8 35.728143452456415 37.0 35.0 37.0 33.0 37.0 9 37.333572145506395 39.0 37.0 39.0 34.0 39.0 10-11 37.276834676457966 39.0 37.0 39.0 34.0 39.0 12-13 37.20613759884439 39.0 37.0 39.0 33.0 39.0 14-15 38.41869346868091 40.0 38.0 41.0 33.5 41.0 16-17 38.396140837928336 40.0 38.0 41.0 33.0 41.0 18-19 38.4101183283303 40.0 38.0 41.0 33.5 41.0 20-21 38.381876947375645 40.0 38.0 41.0 34.0 41.0 22-23 38.26523183583369 40.0 38.0 41.0 34.0 41.0 24-25 38.17233583892274 40.0 37.0 41.0 33.0 41.0 26-27 38.06872266114297 40.0 37.0 41.0 33.0 41.0 28-29 37.948342135655395 40.0 37.0 41.0 33.0 41.0 30-31 37.936054406142745 40.0 37.0 41.0 33.0 41.0 32-33 37.841581463833336 40.0 37.0 41.0 33.0 41.0 34-35 37.693694893412626 40.0 37.0 41.0 33.0 41.0 36-37 37.53478351637744 40.0 36.0 41.0 32.0 41.0 38-39 37.4123397467583 40.0 36.0 41.0 32.0 41.0 40-41 37.29233648441343 40.0 36.0 41.0 31.5 41.0 42-43 37.18183967174181 39.0 36.0 41.0 31.0 41.0 44-45 37.020169839650805 39.0 36.0 41.0 31.0 41.0 46-47 36.76294150691257 39.0 35.0 41.0 30.5 41.0 48-49 35.2107051724158 39.0 35.0 40.0 23.0 41.0 50-51 35.93050449459241 38.5 34.5 40.0 28.0 41.0 52-53 36.02809541864433 39.0 35.0 40.0 28.5 41.0 54-55 35.85851381873458 38.0 35.0 40.0 28.0 41.0 56-57 35.65359640083693 38.0 34.5 40.0 28.0 41.0 58-59 35.423675377495755 38.0 34.0 40.0 27.5 41.0 60-61 35.1702384303062 37.5 34.0 40.0 27.0 41.0 62-63 34.8428490389167 37.0 34.0 40.0 26.0 41.0 64-65 34.50747635236115 36.5 33.0 39.0 26.0 41.0 66-67 34.17230429129192 36.0 33.0 39.0 26.0 40.0 68-69 33.81055298780778 35.5 33.0 39.0 26.0 40.0 70-71 33.44036814485975 35.0 32.0 38.0 25.0 40.0 72-73 33.054209442540284 35.0 32.0 37.0 25.0 39.5 74-75 32.67954977896305 35.0 31.5 37.0 24.0 39.0 76-77 31.74071351145775 34.0 30.5 36.0 23.5 38.0 78-79 32.17935889398949 34.5 31.0 36.0 24.0 38.0 80-81 32.057757896793596 34.5 31.0 35.5 24.0 37.0 82-83 31.781108144696915 34.0 31.0 35.0 24.0 37.0 84-85 31.495591909152125 34.0 31.0 35.0 23.0 36.0 86-87 31.235635215181013 34.0 31.0 35.0 22.5 36.0 88-89 31.00817781465927 34.0 30.5 35.0 20.5 36.0 90-91 30.790781346133045 34.0 30.0 35.0 20.0 35.0 92-93 30.593658373758885 34.0 30.0 35.0 20.0 35.0 94-95 30.396061460018537 34.0 29.5 35.0 19.5 35.0 96-97 30.199183454270766 34.0 29.0 35.0 19.0 35.0 98-99 29.974274142805804 34.0 29.0 35.0 18.5 35.0 100 27.572633985841076 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2853027570283224 1101 2 -0.28325901151970356 1101 3 -0.30538711507885097 1101 4 -0.1981879884129043 1101 5 -0.21797878382245273 1101 6 -0.1952357707706156 1101 7 -0.21198236831698836 1101 8 -0.2726158462213135 1101 9 -0.3618809688436926 1101 10-11 -0.3069344495684305 1101 12-13 -0.4272555652837653 1101 14-15 -0.4987798372517389 1101 16-17 -0.4888478184164242 1101 18-19 -0.48163427376098156 1101 20-21 -0.490872026420071 1101 22-23 -0.49530803750828056 1101 24-25 -0.5315937599995593 1101 26-27 -0.6999762578978732 1101 28-29 -0.5656809039669426 1101 30-31 -0.5605782066527141 1101 32-33 -0.5379567735371324 1101 34-35 -0.6654032437938824 1101 36-37 -0.5226973648985194 1101 38-39 -0.5435595546870502 1101 40-41 -0.4835985591024681 1101 42-43 -0.5282961827251711 1101 44-45 -0.6300718315234661 1101 46-47 -0.5420561920529607 1101 48-49 0.7042629491479957 1101 50-51 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0.9202989237163166 2205 34-35 0.8985302044121539 2205 36-37 0.9198369807050142 2205 38-39 0.9142069028886652 2205 40-41 0.9128588589779767 2205 42-43 1.0147236839465137 2205 44-45 0.9017074463629697 2205 46-47 0.9989648411303804 2205 48-49 2.21606706896322 2205 50-51 1.3909169286256002 2205 52-53 1.0941643507708179 2205 54-55 1.0058842164754722 2205 56-57 0.978945362746714 2205 58-59 1.1019664564090732 2205 60-61 1.1386126099628129 2205 62-63 1.0853183275039981 2205 64-65 1.0804655575349003 2205 66-67 1.0892255912038635 2205 68-69 1.0393748122353585 2205 70-71 1.049365346620931 2205 72-73 1.0559830006735282 2205 74-75 0.9927079230942581 2205 76-77 1.1169210450995415 2205 78-79 0.8691982518502002 2205 80-81 0.7235973552614183 2205 82-83 0.8487503351549286 2205 84-85 0.9500998696169738 2205 86-87 0.9556732452617638 2205 88-89 0.9846484605816421 2205 90-91 1.0015255925626683 2205 92-93 0.9671957165220242 2205 94-95 1.0426362377525606 2205 96-97 1.0571214268222384 2205 98-99 1.1220139410665482 2205 100 1.3316011429357069 2206 1 0.5301334264863158 2206 2 0.5080848710932173 2206 3 0.48273754129469637 2206 4 0.3525221575158213 2206 5 0.41476628959741646 2206 6 0.42152948548752533 2206 7 0.47847774613804006 2206 8 0.4567113930633795 2206 9 0.6005171576263066 2206 10-11 0.5969209909113005 2206 12-13 0.5641636693261844 2206 14-15 0.7468021203571737 2206 16-17 0.6677264626342492 2206 18-19 0.718842420486574 2206 20-21 0.6471879428777285 2206 22-23 0.7581236047164737 2206 24-25 0.7754118410590962 2206 26-27 0.7520396223295975 2206 28-29 0.7596037334534671 2206 30-31 0.7797664089739484 2206 32-33 0.8255898027542088 2206 34-35 0.8898982810125204 2206 36-37 0.9324494134968546 2206 38-39 0.9063448139975065 2206 40-41 0.889634228143187 2206 42-43 0.9275634813659508 2206 44-45 0.8731330235453783 2206 46-47 0.9052914529492071 2206 48-49 2.0736793502001305 2206 50-51 1.2941252173254725 2206 52-53 1.1035398284106606 2206 54-55 1.0439511044338232 2206 56-57 0.9371789869150149 2206 58-59 0.967680702507181 2206 60-61 0.9874220009506516 2206 62-63 0.9456244813432093 2206 64-65 0.8903793131109907 2206 66-67 1.0325634948316988 2206 68-69 0.9817422484355731 2206 70-71 0.9719976234337366 2206 72-73 0.9586936733000684 2206 74-75 1.026990645897726 2206 76-77 1.0481235592344582 2206 78-79 0.9841373751404561 2206 80-81 0.8743177570689866 2206 82-83 0.9374037729042222 2206 84-85 0.9451812352622504 2206 86-87 0.9571716397469743 2206 88-89 0.9929838845130057 2206 90-91 1.0514345628727355 2206 92-93 0.9962847166658442 2206 94-95 0.9461559879384573 2206 96-97 0.9851031731605744 2206 98-99 1.0855353164371344 2206 100 1.1984602736037822 2207 1 0.5361118132388185 2207 2 0.49805830270023677 2207 3 0.47289163478465923 2207 4 0.3352288485967634 2207 5 0.36704517413686233 2207 6 0.3725377568649293 2207 7 0.4359097135505934 2207 8 0.41989723214845753 2207 9 0.5394087906622218 2207 10-11 0.5471436256193556 2207 12-13 0.6221866316574776 2207 14-15 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1.0003127399868745 2209 68-69 1.042049200033425 2209 70-71 1.0393495272181 2209 72-73 1.0223343565217746 2209 74-75 1.042933599162545 2209 76-77 0.9402034367987078 2209 78-79 0.8972330878134898 2209 80-81 0.90738496313989 2209 82-83 0.8567786820913206 2209 84-85 0.9850013951142422 2209 86-87 0.9867891531047803 2209 88-89 0.850682596446763 2209 90-91 0.9969595958314983 2209 92-93 1.0824472655052197 2209 94-95 0.9273047598286901 2209 96-97 1.120096184891608 2209 98-99 1.283062757235104 2209 100 1.463803994779834 2210 1 0.5990149565992198 2210 2 0.48039362701680943 2210 3 0.5276044718672495 2210 4 0.33791227534270973 2210 5 0.3843935421416944 2210 6 0.4558309592220269 2210 7 0.46566255670054346 2210 8 0.45084926445412066 2210 9 0.6224552499699243 2210 10-11 0.606845643032031 2210 12-13 0.6188468568700856 2210 14-15 0.8135500693813356 2210 16-17 0.7945151118716893 2210 18-19 0.8486300668264875 2210 20-21 0.8622153499804384 2210 22-23 0.7831876399427671 2210 24-25 0.8068598141021184 2210 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1.103012307126626 2310 98-99 1.127257781689341 2310 100 1.3399736405420448 2311 1 0.3603249049420114 2311 2 0.3501164662273837 2311 3 0.3925886282856794 2311 4 0.2627500187276297 2311 5 0.29268747878774803 2311 6 0.35466064836178646 2311 7 0.42378035449365115 2311 8 0.14419712531589113 2311 9 0.3878635151232146 2311 10-11 0.3798220203028677 2311 12-13 0.4459070955936326 2311 14-15 0.4247388983827278 2311 16-17 0.4327422100000007 2311 18-19 0.5668634310658476 2311 20-21 0.6681757333849205 2311 22-23 0.626663815984827 2311 24-25 0.6786358235573218 2311 26-27 0.7156618863569122 2311 28-29 0.7790986948248602 2311 30-31 0.6015124377842298 2311 32-33 0.647105764136171 2311 34-35 0.7642715569930374 2311 36-37 0.8292463702391544 2311 38-39 0.8291411736081642 2311 40-41 0.8046749440670524 2311 42-43 0.8812182705731999 2311 44-45 0.8806437375668779 2311 46-47 0.8658227019584004 2311 48-49 -5.844834175096054 2311 50-51 -0.9707234124423394 2311 52-53 0.40185537549951533 2311 54-55 0.7863870184679982 2311 56-57 0.9445121467454953 2311 58-59 0.9641845098820241 2311 60-61 0.7682622655909412 2311 62-63 0.7288694559254836 2311 64-65 0.8185812334441138 2311 66-67 0.9324941377258327 2311 68-69 0.9694671636217365 2311 70-71 0.9200737233713809 2311 72-73 0.36093001061075114 2311 74-75 0.7097363814265307 2311 76-77 0.683425797685512 2311 78-79 0.6807579006163067 2311 80-81 0.7957842437154241 2311 82-83 0.7503460730764964 2311 84-85 0.8086322863196465 2311 86-87 0.8517612570542781 2311 88-89 0.8582090242840898 2311 90-91 0.9084501992671186 2311 92-93 0.8902131956182693 2311 94-95 0.939851559123948 2311 96-97 1.0120145889438739 2311 98-99 1.0458674114708977 2311 100 1.0825897824762052 2312 1 0.17244956777472709 2312 2 0.3246514298387666 2312 3 0.22487016933102666 2312 4 0.010006876582991708 2312 5 0.21090850784775483 2312 6 0.13986027684160973 2312 7 0.2654790414497299 2312 8 0.3419711850644731 2312 9 0.28089751176426603 2312 10-11 0.2344386674604806 2312 12-13 0.30709627824924013 2312 14-15 0.37047913961637846 2312 16-17 0.4721831484701511 2312 18-19 0.33533510901383323 2312 20-21 0.40130814470357024 2312 22-23 0.591304828250081 2312 24-25 0.650209440535285 2312 26-27 0.599787032674783 2312 28-29 0.7459834599528605 2312 30-31 0.7553669596428492 2312 32-33 0.7711728286178214 2312 34-35 0.4430860004761641 2312 36-37 0.789779030934362 2312 38-39 0.8211506270154558 2312 40-41 0.5950733103961241 2312 42-43 0.646186589571613 2312 44-45 0.818486948417295 2312 46-47 0.8449759031391224 2312 48-49 -4.47499124415809 2312 50-51 -0.7035984951437513 2312 52-53 0.6466206943452519 2312 54-55 0.924962454876983 2312 56-57 0.9472288880554842 2312 58-59 0.9808402557215032 2312 60-61 0.9983872083361263 2312 62-63 0.7485972410544974 2312 64-65 0.9484523464778576 2312 66-67 1.0041765388658348 2312 68-69 0.816162606137901 2312 70-71 0.8616133691548811 2312 72-73 1.0439393240618173 2312 74-75 0.7328542881902322 2312 76-77 0.8940860094011178 2312 78-79 0.502231639737893 2312 80-81 0.687594895657007 2312 82-83 0.6904848842119229 2312 84-85 0.7695374214410258 2312 86-87 0.9783949279790107 2312 88-89 0.9788124901093234 2312 90-91 0.7852130116239806 2312 92-93 0.9760187709656201 2312 94-95 1.0833579080475566 2312 96-97 1.1535997205027329 2312 98-99 1.1867403566589516 2312 100 0.7904655013423074 2313 1 0.00925024659202478 2313 2 0.168821173629631 2313 3 0.2743494631834409 2313 4 0.050682758589154275 2313 5 0.10486525791126411 2313 6 0.2592673723570229 2313 7 0.1503828797767639 2313 8 0.21699406308209035 2313 9 0.40938847525563204 2313 10-11 0.19405045648446162 2313 12-13 -0.03400633120141805 2313 14-15 0.018597777245844327 2313 16-17 -0.022803420715192146 2313 18-19 0.35203723352450567 2313 20-21 0.3446560065030724 2313 22-23 0.4175779190147608 2313 24-25 0.21540432917915808 2313 26-27 0.31908090975261416 2313 28-29 0.3748723529527993 2313 30-31 0.5080121148063697 2313 32-33 0.345910872232821 2313 34-35 0.35241776503114863 2313 36-37 -0.436595315099666 2313 38-39 0.06818881788842646 2313 40-41 0.22494003823528175 2313 42-43 0.3803606129700938 2313 44-45 0.3237258294540766 2313 46-47 0.14094672040850753 2313 48-49 -5.389438804781758 2313 50-51 -1.5264075703785096 2313 52-53 -0.04184693480146251 2313 54-55 0.12625472371033908 2313 56-57 0.07258081486556023 2313 58-59 0.10722193998758911 2313 60-61 0.5003985622461045 2313 62-63 0.5726721921535045 2313 64-65 0.6492744751324864 2313 66-67 0.7116219461192159 2313 68-69 0.699926120201404 2313 70-71 0.6787509776558878 2313 72-73 0.4425586264487791 2313 74-75 0.5663171814023116 2313 76-77 0.6771824843364307 2313 78-79 0.586238178780981 2313 80-81 -0.3309414168752163 2313 82-83 0.13780519619161424 2313 84-85 0.5562377083104018 2313 86-87 0.6487838049458361 2313 88-89 0.7436328822545768 2313 90-91 0.7086714895636526 2313 92-93 0.7828963593882285 2313 94-95 0.6650633871786091 2313 96-97 0.8229245538200303 2313 98-99 0.8474573204315163 2313 100 0.9368268482223883 2314 1 0.034258676867409577 2314 2 0.18926648793819822 2314 3 0.2330272876682855 2314 4 0.02195338339858921 2314 5 0.18397917112029916 2314 6 0.24791855766108029 2314 7 0.09687901751155437 2314 8 0.20829362735064905 2314 9 0.4297737131460124 2314 10-11 0.2940876647100623 2314 12-13 0.2497032689367913 2314 14-15 0.23093506314254597 2314 16-17 0.4726545084176408 2314 18-19 0.4978594019430602 2314 20-21 0.5612155878719918 2314 22-23 0.2513839106035789 2314 24-25 0.42907357411524316 2314 26-27 0.5394309338076511 2314 28-29 0.6466460168214496 2314 30-31 0.46948129034256425 2314 32-33 0.4586651936972075 2314 34-35 0.29538542262835676 2314 36-37 0.3730469867252353 2314 38-39 0.4839101680441331 2314 40-41 0.31076293344390393 2314 42-43 0.3131903444101525 2314 44-45 0.5261153292218452 2314 46-47 0.5607106262028125 2314 48-49 -6.1122909933008245 2314 50-51 -1.4484108827793065 2314 52-53 -0.8041260163150028 2314 54-55 -0.3591875733182448 2314 56-57 0.024909871149453977 2314 58-59 0.4576484825566922 2314 60-61 0.7074005483162082 2314 62-63 0.5979502933569378 2314 64-65 0.7235417753055984 2314 66-67 0.7852553238430247 2314 68-69 0.7100420835946792 2314 70-71 0.5119040205570542 2314 72-73 0.7178815096665829 2314 74-75 0.7268421660956648 2314 76-77 0.6249046826585989 2314 78-79 0.4231801961055126 2314 80-81 0.579831214804706 2314 82-83 0.5657419419633847 2314 84-85 0.5571011691721459 2314 86-87 0.6374359079141243 2314 88-89 0.5659626873524779 2314 90-91 0.7087578111787813 2314 92-93 0.7158710205988328 2314 94-95 0.7089679921890131 2314 96-97 0.7846719039728036 2314 98-99 0.8598205211660392 2314 100 -0.3734579409179517 2315 1 0.09867499386975709 2315 2 0.18242047350237556 2315 3 0.30099104390480846 2315 4 0.13047527722098096 2315 5 0.19201298447008242 2315 6 0.2535675153922057 2315 7 0.2839041750706244 2315 8 0.25552297766569154 2315 9 0.4382968494613806 2315 10-11 0.22096981620113354 2315 12-13 0.40425368016541796 2315 14-15 0.173347706750981 2315 16-17 0.3613008269475202 2315 18-19 0.4082364352158052 2315 20-21 0.3325918875866378 2315 22-23 0.3655852154160897 2315 24-25 0.3941685359037095 2315 26-27 0.5124848548352929 2315 28-29 0.6824591712163013 2315 30-31 0.5899112611244917 2315 32-33 0.6135680100389607 2315 34-35 0.6582332627979568 2315 36-37 0.5739493289706559 2315 38-39 0.5949954953959633 2315 40-41 0.7334173198947838 2315 42-43 0.730808675420505 2315 44-45 0.7552804275456779 2315 46-47 0.6513673592600142 2315 48-49 -5.012058963829865 2315 50-51 -0.8210563890598621 2315 52-53 0.343231456951294 2315 54-55 0.2671754231947361 2315 56-57 0.633307942020771 2315 58-59 0.786981475788032 2315 60-61 0.6277205097453304 2315 62-63 0.366230349322727 2315 64-65 0.5299500424152725 2315 66-67 -0.05392089357471974 2315 68-69 0.6108078247015314 2315 70-71 0.5381942581436405 2315 72-73 0.6345340460210736 2315 74-75 0.7353034213887497 2315 76-77 0.7194675334344645 2315 78-79 0.6859977291917616 2315 80-81 0.5754872401281759 2315 82-83 0.6119600613601328 2315 84-85 0.6494403229759591 2315 86-87 0.6402446119915268 2315 88-89 0.6693557651074471 2315 90-91 0.6851263867288111 2315 92-93 0.8494270768871743 2315 94-95 0.396436671012129 2315 96-97 0.5841394470111752 2315 98-99 0.6007196256209646 2315 100 0.8512877195535502 2316 1 0.23007845217321687 2316 2 0.287286970150447 2316 3 0.24988696638984464 2316 4 -0.010357953018079513 2316 5 0.12508157668926856 2316 6 0.16091309384048458 2316 7 0.2539387700293929 2316 8 0.30864733022126245 2316 9 0.481761144810811 2316 10-11 0.4551543479867135 2316 12-13 0.3106375008738027 2316 14-15 0.40108828778531347 2316 16-17 0.43396700869849525 2316 18-19 0.19374464773010658 2316 20-21 0.4812233919034341 2316 22-23 0.566985769690632 2316 24-25 0.6129935121423458 2316 26-27 0.6552796709076603 2316 28-29 0.673148330319826 2316 30-31 0.5734349959093947 2316 32-33 0.5557036811031608 2316 34-35 0.484105676311799 2316 36-37 0.37331067860630185 2316 38-39 0.4784632611717399 2316 40-41 0.09372048660294041 2316 42-43 0.06336035545962204 2316 44-45 0.35118151120307317 2316 46-47 0.3141122826746354 2316 48-49 -5.119065908657291 2316 50-51 -1.101330766382901 2316 52-53 0.17326863050887908 2316 54-55 0.4050613373378269 2316 56-57 0.35690518922989156 2316 58-59 0.2812793069330297 2316 60-61 0.5250153906552981 2316 62-63 0.38131583558733695 2316 64-65 0.3787237919811517 2316 66-67 0.3603465955606282 2316 68-69 0.043783073133880634 2316 70-71 -0.19557193448812882 2316 72-73 -0.08047271596928596 2316 74-75 0.07569647636258736 2316 76-77 0.11303642599334296 2316 78-79 0.28270269972666995 2316 80-81 0.2541289892826768 2316 82-83 0.11290939810377765 2316 84-85 0.2430702895734207 2316 86-87 0.24244475547887134 2316 88-89 0.10243280360408846 2316 90-91 0.19831742264486607 2316 92-93 0.28184772783680856 2316 94-95 0.4298203342222813 2316 96-97 0.45304856307843266 2316 98-99 0.4755810073027753 2316 100 -0.3688857421433269 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 3.0 12 24.0 13 94.0 14 351.0 15 1107.0 16 2761.0 17 5351.0 18 9125.0 19 13358.0 20 17216.0 21 23470.0 22 29071.0 23 31322.0 24 31941.0 25 34097.0 26 39706.0 27 47471.0 28 58295.0 29 71782.0 30 87904.0 31 108780.0 32 135510.0 33 175623.0 34 243339.0 35 338837.0 36 510197.0 37 749772.0 38 598192.0 39 74532.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.69704956519893 19.020230996158208 13.75902024263002 23.523699196012842 2 15.986846830239031 20.40977316917362 40.06860955736135 23.534770443225998 3 18.710489113589563 26.253081347576007 30.339214280355964 24.697215258478465 4 12.852655916011111 16.84503642523738 37.2994430792049 33.00286457954661 5 13.007043148333535 37.07054691618383 34.23060808105537 15.691801854427256 6 30.262182695436174 36.188486826897666 18.8490025275911 14.70032795007506 7 27.2874741839842 32.62481460298944 22.181638332411133 17.906072880615227 8 26.3195802396263 35.095293688328795 20.228664143723442 18.35646192832147 9 27.113667524997492 16.225011595463013 20.59485088743459 36.066469992104906 10-11 25.275717228248794 26.038522777892144 28.077870744810145 20.60788924904891 12-13 25.3976605296519 24.025089845430966 28.23570032914248 22.341549295774648 14-15 23.044158706459346 25.263208395453358 26.405233129097468 25.287399768989825 16-17 22.206834408695318 27.84728381749459 27.20754750446285 22.73833426934725 18-19 21.84259416351299 26.935836325633822 29.39118733523811 21.830382175615075 20-21 22.806362564774446 26.331616094476633 29.17728673934547 21.68473460140345 22-23 22.680280928630523 26.07760472418806 29.17563244051405 22.066481906667367 24-25 22.235616640694385 26.022059793091124 29.42645671933377 22.315866846880716 26-27 22.081249601111175 26.16893870349029 29.250344879823064 22.49946681557547 28-29 22.067855398789927 26.61968495385787 29.31214882020161 22.00031082715059 30-31 22.726793619307525 26.302554129188366 29.32727836026072 21.64337389124339 32-33 22.57176854705368 26.362033579322702 29.083861651189828 21.982336222433787 34-35 22.576510841004897 26.507612914108066 29.107243864809885 21.808632380077153 36-37 22.536917106262493 26.444660657520085 28.900440179899235 22.11798205631819 38-39 22.46049063992934 26.64160042390713 28.895686448320763 22.002222487842772 40-41 22.532678664787877 26.212223335564538 29.216008634832313 22.03908936481527 42-43 22.37280624660063 26.52239154686266 28.72991690012473 22.37488530641198 44-45 22.05387117269977 26.819911339088005 28.889833946166632 22.236383542045598 46-47 22.087972227989642 26.426185669777585 28.541931652891744 22.943910449341033 48-49 22.35510988381187 26.6673709887667 28.354771750346853 22.62274737707458 50-51 22.20465218672784 26.883303023204512 28.34384554615595 22.568199243911693 52-53 22.21487271153177 26.98299742644338 28.048088386453134 22.754041475571714 54-55 22.140617239578425 26.825963586284544 28.296336295887897 22.737082878249126 56-57 22.39336606585955 26.82832289178348 27.89786812097174 22.880442921385235 58-59 22.032519048586945 26.859503531436314 28.333535428786945 22.7744419911898 60-61 22.46864198579094 26.98097714122381 28.13291546879057 22.41746540419468 62-63 22.460159883873086 26.942092982951998 28.003303871246143 22.594443261928774 64-65 22.187768686083338 26.700480732249687 28.04311685962505 23.068633722041927 66-67 22.127119844410714 26.66940155885947 27.967956680703708 23.23552191602611 68-69 21.75728402555395 26.71098999809353 28.193381922345512 23.33834405400701 70-71 22.017045206675455 26.4642625929724 28.211870336838274 23.306821863513868 72-73 22.02648027444653 26.94449065343913 27.678331497252863 23.350697574861478 74-75 22.162488314037258 26.947706498049552 27.535646729893237 23.35415845801995 76-77 21.988423219862856 26.756160615101866 27.695665323166935 23.559750841868336 78-79 21.872354779099066 26.747048911233982 27.717931308220304 23.662665001446648 80-81 21.9004768027523 26.916326612502246 27.511952053380504 23.671244531364945 82-83 22.22927571941188 26.82000704606565 27.65702904662777 23.293688187894695 84-85 22.22616616784883 26.984149604052742 27.254284835986308 23.535399392112115 86-87 21.84743127799996 27.015324517788887 27.566057328037047 23.57118687617411 88-89 21.84505058325696 26.62552683924375 27.60623094747735 23.923191630021932 90-91 22.233769338349763 26.53756548145464 27.566903557846302 23.661761622349296 92-93 22.119035942141174 26.681562286581862 27.454720276746215 23.744681494530752 94-95 21.76039525046928 26.50345612991088 27.566588820205208 24.169559799414632 96-97 21.792808806198558 26.489064217825298 27.602458533759915 24.11566844221623 98-99 21.714834013025605 26.471400360922487 27.407934740235994 24.405830885815917 100 21.608598239899433 26.543680097635352 27.503367413212942 24.344354249252277 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1848.0 1 1323.5 2 2126.5 3 3089.0 4 1964.0 5 1253.5 6 1744.0 7 3165.0 8 4854.5 9 5595.5 10 4781.5 11 3693.5 12 3574.5 13 3855.0 14 4191.5 15 4621.5 16 4991.5 17 5309.5 18 5759.0 19 6245.0 20 6698.5 21 7197.0 22 7962.5 23 9266.5 24 10757.5 25 12564.0 26 15307.0 27 18664.5 28 22312.0 29 27040.0 30 33438.5 31 39540.0 32 46719.0 33 55187.0 34 63360.0 35 71239.5 36 80585.0 37 90443.0 38 95913.5 39 98576.5 40 98994.0 41 99548.0 42 102339.5 43 106174.0 44 111360.5 45 119046.5 46 125172.0 47 128887.5 48 134078.0 49 138386.5 50 139456.0 51 138052.0 52 135009.0 53 130765.5 54 125324.5 55 116559.0 56 105897.0 57 94473.0 58 83013.0 59 71406.0 60 58252.0 61 46020.5 62 36683.0 63 28705.5 64 21213.5 65 15374.0 66 11211.0 67 8518.0 68 6464.0 69 4592.5 70 3398.0 71 2621.0 72 1992.5 73 1481.5 74 1084.5 75 728.0 76 472.5 77 269.5 78 152.0 79 89.0 80 56.5 81 34.5 82 19.0 83 10.0 84 8.5 85 6.5 86 4.0 87 2.0 88 1.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029221538221771995 2 0.002122559492725727 3 5.815231486919799E-5 4 0.020178853259611707 5 4.36142361518985E-4 6 2.9076157434598995E-5 7 8.7228472303797E-5 8 0.004041585883409261 9 0.011601386816405001 10-11 9.304370379071679E-4 12-13 1.1630462973839598E-4 14-15 4.36142361518985E-5 16-17 4.216042828016855E-4 18-19 1.4538078717299498E-5 20-21 5.815231486919799E-5 22-23 2.180711807594925E-4 24-25 1.4538078717299498E-5 26-27 7.26903935864975E-5 28-29 9.740512740590664E-4 30-31 0.0025441637755274126 32-33 0.009944045842632858 34-35 0.01974271089809272 36-37 0.02219964620131634 38-39 0.021952498863122245 40-41 0.00436142361518985 42-43 0.005509931833856511 44-45 0.0086937710729451 46-47 0.011223396769755213 48-49 1.1972834727632002 50-51 0.018158060317907076 52-53 0.01795452721586488 54-55 0.00930437037907168 56-57 0.007530724775561141 58-59 0.007995943294514725 60-61 0.004972022921316429 62-63 0.007210887043780551 64-65 0.018477898049687663 66-67 0.027942187294649635 68-69 0.02760781148415175 70-71 0.02494734307888594 72-73 0.02209787965029524 74-75 0.008403009498599111 76-77 0.013491337049653936 78-79 0.007530724775561141 80-81 0.0025005495393755142 82-83 0.012953428137113854 84-85 0.022708478956421817 86-87 0.021356437635712964 88-89 0.030559041463763547 90-91 0.019626406268354324 92-93 0.012371904988421875 94-95 0.00609145498254849 96-97 0.003518215049586479 98-99 0.016151805454919746 100 0.033292200262615854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3439244.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.33981354257448 #Duplication Level Percentage of deduplicated Percentage of total 1 80.01455383557112 52.28136028308504 2 12.8263349054376 16.761406623118148 3 3.436922650871393 6.73703655504563 4 1.300673468774852 3.399430477180896 5 0.6911139665889793 2.2578628856796477 6 0.4123802839184344 1.6166910515918658 7 0.2549211393401142 1.16595497987806 8 0.1973486248449717 1.0315777880203147 9 0.12570782999900193 0.7392353555678792 >10 0.6777655110326247 7.74664227251727 >50 0.040106121553028826 1.7959197793264194 >100 0.02084915190255392 2.436319553196329 >500 8.138524096675822E-4 0.35090149575937896 >1k 3.560604289781208E-4 0.5517110083610053 >5k 5.0865775568302975E-5 0.2585065194597529 >10k+ 1.0173155113660595E-4 0.869443372212283 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14114 0.41038088603193024 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12046 0.35025139245717957 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7778 0.22615435252631103 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4334 0.12601606632155207 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.9076157434599E-5 7 0.0 0.0 0.0 0.0 2.9076157434599E-5 8 0.0 0.0 0.0 0.0 2.9076157434599E-5 9 0.0 0.0 0.0 0.0 5.8152314869198E-5 10-11 0.0 0.0 0.0 2.9076157434599E-5 5.8152314869198E-5 12-13 0.0 1.45380787172995E-5 0.0 2.9076157434599E-5 5.8152314869198E-5 14-15 0.0 2.9076157434599E-5 0.0 2.9076157434599E-5 1.308427084556955E-4 16-17 0.0 2.9076157434599E-5 0.0 2.9076157434599E-5 2.32609259476792E-4 18-19 0.0 2.9076157434599E-5 0.0 5.8152314869198E-5 2.32609259476792E-4 20-21 0.0 2.9076157434599E-5 0.0 1.308427084556955E-4 2.32609259476792E-4 22-23 0.0 2.9076157434599E-5 0.0 3.925281253670865E-4 2.61685416911391E-4 24-25 0.0 2.9076157434599E-5 0.0 0.0013229651632742545 2.9076157434599E-4 26-27 0.0 2.9076157434599E-5 0.0 0.0026023160903966103 3.19837731780589E-4 28-29 0.0 2.9076157434599E-5 0.0 0.006353140399459882 3.48913889215188E-4 30-31 0.0 2.9076157434599E-5 0.0 0.019219340064269938 3.634519679324875E-4 32-33 0.0 2.9076157434599E-5 0.0 0.049909224236489184 4.36142361518985E-4 34-35 0.0 2.9076157434599E-5 0.0 0.09468650668577164 4.65218518953584E-4 36-37 0.0 2.9076157434599E-5 0.0 0.16228857272121433 4.65218518953584E-4 38-39 0.0 2.9076157434599E-5 0.0 0.26593053589684246 4.65218518953584E-4 40-41 0.0 2.9076157434599E-5 0.0 0.3868873508247743 5.088327551054826E-4 42-43 0.0 2.9076157434599E-5 0.0 0.50249415278474 5.23370833822782E-4 44-45 0.0 2.9076157434599E-5 0.0 0.6371749140218025 5.23370833822782E-4 46-47 0.0 2.9076157434599E-5 0.0 0.7937790979645527 5.23370833822782E-4 48-49 0.0 2.9076157434599E-5 0.0 0.9639909235866952 5.23370833822782E-4 50-51 0.0 2.9076157434599E-5 0.0 1.1516484436695973 6.10599306126579E-4 52-53 0.0 2.9076157434599E-5 0.0 1.3482759583210728 6.832896997130764E-4 54-55 0.0 2.9076157434599E-5 0.0 1.5710720146636876 7.55980093299574E-4 56-57 0.0 2.9076157434599E-5 0.0 1.8112119989160407 7.55980093299574E-4 58-59 0.0 2.9076157434599E-5 0.0 2.0864032909558032 7.55980093299574E-4 60-61 0.0 2.9076157434599E-5 0.0 2.3620307253570845 7.85056250734173E-4 62-63 0.0 2.9076157434599E-5 0.0 2.644185757102433 7.85056250734173E-4 64-65 0.0 2.9076157434599E-5 0.0 2.925323123337571 7.85056250734173E-4 66-67 0.0 2.9076157434599E-5 0.0 3.2508743200540584 8.14132408168772E-4 68-69 0.0 2.9076157434599E-5 0.0 3.6076096956191535 8.577466443206705E-4 70-71 0.0 2.9076157434599E-5 0.0 3.9820815272193544 9.01360880472569E-4 72-73 0.0 2.9076157434599E-5 0.0 4.334455479169259 9.740512740590665E-4 74-75 0.0 2.9076157434599E-5 0.0 4.690304031932599 9.88589352776366E-4 76-77 0.0 2.9076157434599E-5 0.0 5.0968614032618795 0.0010176655102109651 78-79 0.0 2.9076157434599E-5 0.0 5.528366117670046 0.001046741667645564 80-81 0.0 2.9076157434599E-5 0.0 5.966921800256102 0.001075817825080163 82-83 0.0 2.9076157434599E-5 0.0 6.405724630180353 0.001133970139949361 84-85 0.0 2.9076157434599E-5 0.0 6.864212018687828 0.001133970139949361 86-87 0.0 2.9076157434599E-5 0.0 7.33826968950153 0.001133970139949361 88 0.0 2.9076157434599E-5 0.0 7.704629273177478 0.001133970139949361 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6985 0.0 51.609768 1 TACCTGG 3665 0.0 48.650665 2 ACCTGGG 4250 0.0 41.186 3 GTACCTG 4735 0.0 38.364372 1 GTATCAA 11820 0.0 37.40852 1 CCTGGGG 4060 0.0 30.514593 4 CAACGCA 14760 0.0 29.559696 5 ATCAACG 14770 0.0 29.516504 3 TCAACGC 15050 0.0 29.029722 4 TATCAAC 15190 0.0 29.005943 2 AACGCAG 15505 0.0 28.411285 6 CTGGGGG 2615 0.0 26.731169 5 GTGGTAT 3420 0.0 26.626417 1 GTACATG 13745 0.0 26.39805 1 TATCACG 345 0.0 25.841259 2 TGGGGGG 5880 0.0 25.53111 6 GTACACG 635 0.0 25.132883 1 TACATGG 14215 0.0 25.119846 2 TAACGCA 415 0.0 24.87736 4 ACGCAGA 17715 0.0 24.655033 7 >>END_MODULE