FastQCFastQC Report
Mon 6 Jun 2016
ERR1211328_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211328_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9222765
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478240.5185429749104525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369910.4010836229699011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213500.23149239951359488No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG103960.11272107659687741No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC95920.10400351738334436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA217100.051.868771
GTATCAA423800.042.1869931
CAACGCA501700.034.446675
ATCAACG504950.034.2729723
GTACTAG66200.033.7502671
TATCAAC543000.032.465352
TACCCCG4850.031.9706755
AACGCAG553300.031.9212176
TAGGCAT84750.031.5465725
TACCTGG28600.030.2602272
TCAACGC580250.030.0272774
GCATCGT67000.029.9454158
ACGCAGA593100.029.0596527
CGCAGAG594800.028.8573178
ACCTGGG29900.028.1306823
TCAAGAC15650.027.923323
AAGCTAA49350.027.8985259
CTGTGCG76100.027.5391799
GCAGAGT623100.027.0429069
CTAGGCA93250.027.0089284
Produced by FastQC (version 0.11.5)