##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211328_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9222765 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.57847868833262 31.0 31.0 34.0 30.0 34.0 2 31.60528095424745 33.0 31.0 34.0 30.0 34.0 3 31.650439971093267 33.0 31.0 34.0 28.0 34.0 4 35.27742927419272 37.0 35.0 37.0 33.0 37.0 5 35.34013172839165 37.0 35.0 37.0 33.0 37.0 6 35.40619358728104 37.0 35.0 37.0 33.0 37.0 7 35.34277713895995 37.0 35.0 37.0 32.0 37.0 8 35.35179493351506 37.0 35.0 37.0 32.0 37.0 9 36.91673755104895 39.0 37.0 39.0 33.0 39.0 10-11 36.86005411609209 39.0 37.0 39.0 32.5 39.0 12-13 36.785735514241125 39.0 37.0 39.0 32.0 39.0 14-15 37.911601509959326 40.0 38.0 41.0 32.0 41.0 16-17 37.929620075975045 40.0 38.0 41.0 32.5 41.0 18-19 37.98931112307426 40.0 38.0 41.0 33.0 41.0 20-21 37.973445436374014 40.0 38.0 41.0 33.0 41.0 22-23 37.84862007218009 40.0 37.5 41.0 32.0 41.0 24-25 37.74353417874141 40.0 37.0 41.0 32.0 41.0 26-27 37.59367592039914 40.0 37.0 41.0 32.0 41.0 28-29 37.48512192384821 40.0 37.0 41.0 32.0 41.0 30-31 37.30276489751175 40.0 36.0 41.0 31.0 41.0 32-33 37.173843418974684 39.0 36.0 41.0 31.0 41.0 34-35 37.04986319178684 39.0 36.0 41.0 31.0 41.0 36-37 36.96219192400544 39.0 35.5 41.0 30.0 41.0 38-39 36.818811495251154 39.0 35.0 41.0 30.0 41.0 40-41 36.65443649491232 39.0 35.0 41.0 30.0 41.0 42-43 36.50056208740004 39.0 35.0 41.0 30.0 41.0 44-45 36.313778026437845 39.0 35.0 41.0 29.0 41.0 46-47 36.08879419566692 39.0 35.0 41.0 28.0 41.0 48-49 35.80154861367497 39.0 35.0 40.0 27.0 41.0 50-51 35.093703677801614 38.0 34.0 39.5 26.5 40.5 52-53 35.298257518217156 38.0 34.0 40.0 26.5 40.5 54-55 35.650502425248824 39.0 35.0 40.0 27.0 41.0 56-57 35.53223642801264 38.0 34.5 40.0 27.0 41.0 58-59 35.3019862264733 38.0 34.0 40.0 26.0 41.0 60-61 35.064021472953065 38.0 34.0 40.0 26.0 41.0 62-63 34.79571804117312 37.0 34.0 40.0 26.0 41.0 64-65 34.540306730140045 37.0 34.0 40.0 26.0 41.0 66-67 34.34692849703966 36.0 33.5 40.0 25.5 41.0 68-69 34.039902675607586 36.0 33.0 39.0 25.0 41.0 70-71 33.76788181201624 35.0 33.0 39.0 25.0 41.0 72-73 33.444086561893315 35.0 33.0 38.5 24.0 40.5 74-75 33.07366424277318 35.0 32.0 37.5 23.5 40.0 76-77 32.74361566189749 35.0 32.0 37.0 23.0 39.0 78-79 32.38542411088215 35.0 32.0 36.5 22.0 39.0 80-81 32.06791108740166 35.0 31.0 36.0 21.5 39.0 82-83 31.743874152708003 35.0 31.0 36.0 20.5 37.0 84-85 31.41930885152121 35.0 31.0 35.0 20.0 37.0 86-87 31.174039726698012 34.0 31.0 35.0 20.0 36.5 88-89 30.924879632084306 34.0 30.5 35.0 19.0 36.0 90-91 30.65973409275852 34.0 30.0 35.0 18.5 36.0 92-93 30.41122575496611 34.0 30.0 35.0 18.0 36.0 94-95 30.20417304355039 34.0 29.0 35.0 17.5 35.0 96-97 30.01003327093339 34.0 29.0 35.0 17.0 35.0 98-99 29.776661879599015 34.0 29.0 35.0 15.0 35.0 100 28.051020274288675 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.36139883507065207 1101 2 -0.3148918355464403 1101 3 -0.30559735931137055 1101 4 -0.1853964731288542 1101 5 -0.2597342227917565 1101 6 -0.36405322093563797 1101 7 -0.3743323768487983 1101 8 -0.35184971770659246 1101 9 -0.38319606233848447 1101 10-11 -0.45378681611293814 1101 12-13 -0.42634480283876286 1101 14-15 -0.501148146257826 1101 16-17 -0.5262560144626818 1101 18-19 -0.6489091778947156 1101 20-21 -0.6115189947195745 1101 22-23 -0.5525622899393383 1101 24-25 -0.5635053304046878 1101 26-27 -0.6117563687580585 1101 28-29 -0.654036590878242 1101 30-31 -0.6276436487342494 1101 32-33 -0.6862511469581065 1101 34-35 -0.6648097189173754 1101 36-37 -0.7327907695025146 1101 38-39 -0.7535716244977095 1101 40-41 -0.7583499075283058 1101 42-43 -0.7588887140854652 1101 44-45 -0.7631245816203887 1101 46-47 -0.6476545006459773 1101 48-49 -0.6358600668224668 1101 50-51 -0.606078757825216 1101 52-53 -0.437450202684893 1101 54-55 -0.3576849152291217 1101 56-57 -0.4378536121782375 1101 58-59 -0.4709318428963556 1101 60-61 -0.37124568146748516 1101 62-63 -0.37166824717582614 1101 64-65 -0.4139356613852101 1101 66-67 -0.46011238680745237 1101 68-69 -0.47330029020924513 1101 70-71 -0.45551150050086164 1101 72-73 -0.5702528116093077 1101 74-75 -0.5783442101453815 1101 76-77 -0.6172876777566998 1101 78-79 -0.6305115876893268 1101 80-81 -0.6815816418594558 1101 82-83 -0.7784706056534922 1101 84-85 -0.78324264676748 1101 86-87 -0.8264390288179158 1101 88-89 -0.8108219426091026 1101 90-91 -0.7868721690918434 1101 92-93 -0.8321416752788195 1101 94-95 -0.817457873435103 1101 96-97 -0.8493051630207198 1101 98-99 -0.968965829805871 1101 100 -1.0986134901279598 1102 1 -0.34672568737686404 1102 2 -0.3964746927880505 1102 3 -0.3053501704302626 1102 4 -0.29388555204128863 1102 5 -0.2552230518967775 1102 6 -0.28813134257246986 1102 7 -0.3130861730572789 1102 8 -0.3256332289513324 1102 9 -0.45427578808451585 1102 10-11 -0.35936497100505704 1102 12-13 -0.39116614602753685 1102 14-15 -0.479914045294791 1102 16-17 -0.5671692176482708 1102 18-19 -0.5627010802069279 1102 20-21 -0.5949830470670605 1102 22-23 -0.6222960866296319 1102 24-25 -0.6019001790303022 1102 26-27 -0.5910530758041475 1102 28-29 -0.6950456910426297 1102 30-31 -0.572883311970827 1102 32-33 -0.6074496359637038 1102 34-35 -0.6481178969415495 1102 36-37 -0.6758528859211523 1102 38-39 -0.7709149947295728 1102 40-41 -0.8029582637170094 1102 42-43 -0.7286633401468166 1102 44-45 -0.783707474735813 1102 46-47 -0.7743099002304703 1102 48-49 -0.7349567603569653 1102 50-51 -0.6635948774730167 1102 52-53 -0.5899340188832056 1102 54-55 -0.5470399447111163 1102 56-57 -0.5532370351262657 1102 58-59 -0.5974230674292258 1102 60-61 -0.7406255974232678 1102 62-63 -0.6123406244991827 1102 64-65 -0.6030654779679168 1102 66-67 -0.49380126582158823 1102 68-69 -0.5312964577341859 1102 70-71 -0.6325269278103178 1102 72-73 -0.7124560514071305 1102 74-75 -0.6560581489797528 1102 76-77 -0.7586671684638162 1102 78-79 -0.6695486037005658 1102 80-81 -0.6366984713373611 1102 82-83 -0.7483047573635737 1102 84-85 -0.7718917804804306 1102 86-87 -0.712956322089191 1102 88-89 -0.8006440320778587 1102 90-91 -0.8832069654830939 1102 92-93 -0.9377238692477228 1102 94-95 -0.90088631382514 1102 96-97 -0.9351818403831906 1102 98-99 -0.9554363496514178 1102 100 -0.9706369065610971 1103 1 -0.45401124489445976 1103 2 -0.4571322195479617 1103 3 -0.45570690438080064 1103 4 -0.2769696047084551 1103 5 -0.30100416110350636 1103 6 -0.43858201077033243 1103 7 -0.39739048456356585 1103 8 -0.4030235907138433 1103 9 -0.6108501260696642 1103 10-11 -0.5377144349420817 1103 12-13 -0.7007119193539282 1103 14-15 -0.7676012976499536 1103 16-17 -0.7349384316877803 1103 18-19 -0.7764858066928824 1103 20-21 -0.8376397994369285 1103 22-23 -0.7679663772053331 1103 24-25 -0.7740591166415314 1103 26-27 -0.7869036574398152 1103 28-29 -0.7895760262916909 1103 30-31 -0.7684282559950901 1103 32-33 -0.8342039364010958 1103 34-35 -0.8455469846919215 1103 36-37 -0.8949793255256466 1103 38-39 -0.9007798483175407 1103 40-41 -0.9244057331781121 1103 42-43 -0.8592585352100954 1103 44-45 -0.9518294556523514 1103 46-47 -0.9375138449932408 1103 48-49 -0.9056904160141173 1103 50-51 -0.8239350707808342 1103 52-53 -0.7647342491680362 1103 54-55 -0.7060594376348135 1103 56-57 -0.8006341700992934 1103 58-59 -0.7614611245274077 1103 60-61 -0.7096919904519368 1103 62-63 -0.7125165269165166 1103 64-65 -0.7619251529575806 1103 66-67 -0.7821104407221853 1103 68-69 -0.8129661892443778 1103 70-71 -0.7886938030275843 1103 72-73 -0.8776631521011922 1103 74-75 -0.8111693178374253 1103 76-77 -0.7177427516992836 1103 78-79 -0.7298269884898332 1103 80-81 -0.8804728682989484 1103 82-83 -0.9057379613580601 1103 84-85 -0.8787029646443045 1103 86-87 -0.9292252797281968 1103 88-89 -0.9164827946449456 1103 90-91 -0.8474392038032512 1103 92-93 -0.9353027653679042 1103 94-95 -1.1944921804242377 1103 96-97 -1.1491904849282193 1103 98-99 -1.1446285172863249 1103 100 -1.149999541614548 1104 1 -0.4213959009637982 1104 2 -0.37633466300003704 1104 3 -0.42955766239969506 1104 4 -0.28191570457881454 1104 5 -0.22421448839948965 1104 6 -0.2697507742903298 1104 7 -0.3284939770673816 1104 8 -0.2738887453125045 1104 9 -0.368197543006481 1104 10-11 -0.3578354319656327 1104 12-13 -0.4694066728450821 1104 14-15 -0.5704824792330001 1104 16-17 -0.5087568763316597 1104 18-19 -0.6141309530054571 1104 20-21 -0.5520570546612973 1104 22-23 -0.531558707181027 1104 24-25 -0.6347104387524567 1104 26-27 -0.6351727596299668 1104 28-29 -0.6073607154461484 1104 30-31 -0.6656814285810029 1104 32-33 -0.7133384322537779 1104 34-35 -0.6745824664308415 1104 36-37 -0.714856529491648 1104 38-39 -0.6803683681305586 1104 40-41 -0.7685156380767495 1104 42-43 -0.7505894487345515 1104 44-45 -0.7392005530717469 1104 46-47 -0.807210540659014 1104 48-49 -0.8207529695978693 1104 50-51 -0.7918269082520553 1104 52-53 -0.6425984577646418 1104 54-55 -0.6630430539188765 1104 56-57 -0.6567515581905425 1104 58-59 -0.6975557744096008 1104 60-61 -0.5988819595403285 1104 62-63 -0.7022949632348414 1104 64-65 -0.7199826444187281 1104 66-67 -0.7452219671976295 1104 68-69 -0.6106100489437196 1104 70-71 -0.7007905869934632 1104 72-73 -0.7082384642385477 1104 74-75 -0.7297595713569862 1104 76-77 -0.6836748206040753 1104 78-79 -0.6331555978443362 1104 80-81 -0.6613702148215879 1104 82-83 -0.8067716535174903 1104 84-85 -0.7685493861213644 1104 86-87 -0.8129928100734105 1104 88-89 -0.7322164761780918 1104 90-91 -0.7534863764782109 1104 92-93 -0.8326387467174108 1104 94-95 -0.87336406516431 1104 96-97 -0.9758658963428815 1104 98-99 -0.9838361529753605 1104 100 -1.0646466806012356 1105 1 -0.394066037772415 1105 2 -0.42633267871572755 1105 3 -0.3672672649064239 1105 4 -0.25006873993093137 1105 5 -0.262481750654878 1105 6 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1105 68-69 -0.7362363062393555 1105 70-71 -0.7131522789835074 1105 72-73 -0.7696649195154492 1105 74-75 -0.7728712222040741 1105 76-77 -0.641563396771879 1105 78-79 -0.5987984736208745 1105 80-81 -0.7030033941952212 1105 82-83 -0.769289002031357 1105 84-85 -0.7522248747483822 1105 86-87 -0.8002158556871137 1105 88-89 -0.8408227843496654 1105 90-91 -0.8784773433932038 1105 92-93 -0.955805589414453 1105 94-95 -0.9269826012384002 1105 96-97 -0.9596847564981097 1105 98-99 -0.9170442647934927 1105 100 -1.0778450059451288 1106 1 -0.5020167293523912 1106 2 -0.5372248087445648 1106 3 -0.479931500063568 1106 4 -0.40165823049371596 1106 5 -0.34974369152983087 1106 6 -0.34504127009991237 1106 7 -0.40895798540173445 1106 8 -0.4071212387031693 1106 9 -0.5618725478178135 1106 10-11 -0.5504115774836222 1106 12-13 -0.5359879750058312 1106 14-15 -0.6242350058261366 1106 16-17 -0.7141965698112926 1106 18-19 -0.6962259071179417 1106 20-21 -0.6254118298233422 1106 22-23 -0.6441232297681054 1106 24-25 -0.6802793168401067 1106 26-27 -0.574197731373097 1106 28-29 -0.6110384986061916 1106 30-31 -0.7009747199533507 1106 32-33 -0.7056963379641275 1106 34-35 -0.715213750637318 1106 36-37 -0.7082422004422071 1106 38-39 -0.7489438326604798 1106 40-41 -0.7398799691058073 1106 42-43 -0.7138222898491549 1106 44-45 -0.751817853955906 1106 46-47 -0.7670250144442434 1106 48-49 -0.8084239180889412 1106 50-51 -0.8505734908314935 1106 52-53 -0.6962330680711091 1106 54-55 -0.7422616252692436 1106 56-57 -0.733018865633241 1106 58-59 -0.7269941348326867 1106 60-61 -0.8030048637719887 1106 62-63 -0.7763495758617651 1106 64-65 -0.7175482325559059 1106 66-67 -0.7822108431300734 1106 68-69 -0.7172920120209412 1106 70-71 -0.6974618188278967 1106 72-73 -0.670546738381347 1106 74-75 -0.7937165045419974 1106 76-77 -0.7163708551395978 1106 78-79 -0.7000184358982793 1106 80-81 -0.7568070094541071 1106 82-83 -0.7413431767170451 1106 84-85 -0.7313417195314749 1106 86-87 -0.73109796936712 1106 88-89 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0.543043277384502 2311 4 0.4245772180340168 2311 5 0.37434755476097337 2311 6 0.271453251698766 2311 7 0.3896396521239396 2311 8 0.4463080582609962 2311 9 0.5858993649874833 2311 10-11 0.6151125608409629 2311 12-13 0.6414823514036669 2311 14-15 0.8250183039204941 2311 16-17 0.7680868700426231 2311 18-19 0.6859145859201732 2311 20-21 0.728837770724482 2311 22-23 0.7685262794763545 2311 24-25 0.8048078493320006 2311 26-27 0.8775200556618259 2311 28-29 0.870149871628918 2311 30-31 0.8494247322265096 2311 32-33 0.87816964582953 2311 34-35 0.9014267138684957 2311 36-37 0.8748051917057111 2311 38-39 0.9299543926305489 2311 40-41 0.9689807362126146 2311 42-43 0.9663780068867496 2311 44-45 0.9579608780687536 2311 46-47 1.0132262124446 2311 48-49 0.9805076899914056 2311 50-51 0.9302312626494427 2311 52-53 0.8818843708697699 2311 54-55 0.829885287536051 2311 56-57 0.7460078976628068 2311 58-59 0.7213504212047397 2311 60-61 0.7884920832720681 2311 62-63 0.7981140169252683 2311 64-65 0.7909723677636649 2311 66-67 0.8214281662285146 2311 68-69 0.7789669756531055 2311 70-71 0.7104273617244843 2311 72-73 0.6604540319907102 2311 74-75 0.7102446344673581 2311 76-77 0.7815950772959184 2311 78-79 0.7283020103468729 2311 80-81 0.7990733546180238 2311 82-83 0.8112873585488778 2311 84-85 0.8475376744425027 2311 86-87 0.8043942127830732 2311 88-89 0.8851583642906355 2311 90-91 0.9213951352980985 2311 92-93 0.8933890697346101 2311 94-95 0.9497361349749198 2311 96-97 0.9976613399954388 2311 98-99 1.0723157352947403 2311 100 1.1497558022854086 2312 1 0.5428070373315066 2312 2 0.5294609688734901 2312 3 0.5741127845064646 2312 4 0.44599253356121693 2312 5 0.5122307553077974 2312 6 0.516536047271245 2312 7 0.5305257999432555 2312 8 0.5102350048573001 2312 9 0.6798115384723218 2312 10-11 0.70895928429713 2312 12-13 0.7035187255970285 2312 14-15 0.833671102726889 2312 16-17 0.8244862916128426 2312 18-19 0.815345894626752 2312 20-21 0.8609989446356039 2312 22-23 0.8768571751238028 2312 24-25 0.8776829926532415 2312 26-27 0.919378592094823 2312 28-29 0.912043383954547 2312 30-31 0.9671289466056763 2312 32-33 0.9343717526516215 2312 34-35 0.9161683406012173 2312 36-37 0.9424917573615303 2312 38-39 0.9546805663946856 2312 40-41 0.9730500485647013 2312 42-43 0.9556382120933549 2312 44-45 0.9057038902400265 2312 46-47 0.9206789072512223 2312 48-49 0.9293396678978851 2312 50-51 0.8725540128736071 2312 52-53 0.8059484832336139 2312 54-55 0.7068046068704561 2312 56-57 0.6784882185553656 2312 58-59 0.6952839275035316 2312 60-61 0.7369440945466721 2312 62-63 0.7288490710933502 2312 64-65 0.779455827023682 2312 66-67 0.7418241370644836 2312 68-69 0.7711780823135044 2312 70-71 0.7569602741588852 2312 72-73 0.7841232851768609 2312 74-75 0.7598237294121546 2312 76-77 0.7671078916838994 2312 78-79 0.7920156931145144 2312 80-81 0.8512873828265768 2312 82-83 0.8603723903000393 2312 84-85 0.8774328570117085 2312 86-87 0.8964076004559729 2312 88-89 0.8936493571025288 2312 90-91 0.9468913758719744 2312 92-93 1.0078138554877434 2312 94-95 0.9843604220436681 2312 96-97 0.9792644982073782 2312 98-99 1.0418031322137047 2312 100 1.1842618952751636 2313 1 0.4417537535374336 2313 2 0.44699283476029805 2313 3 0.4846935525279257 2313 4 0.38946130654824174 2313 5 0.4114560607907123 2313 6 0.41225350927985005 2313 7 0.40220770621596813 2313 8 0.43028402711710356 2313 9 0.5271282901718877 2313 10-11 0.5408905727833542 2313 12-13 0.5508089817748143 2313 14-15 0.7263730925869325 2313 16-17 0.7044596601140398 2313 18-19 0.6255497281751801 2313 20-21 0.6353894040226891 2313 22-23 0.6035252550061543 2313 24-25 0.6253472328936525 2313 26-27 0.6771101944043636 2313 28-29 0.6943165382955812 2313 30-31 0.7609009937883897 2313 32-33 0.7558342196499197 2313 34-35 0.7392945718476511 2313 36-37 0.7932889026294916 2313 38-39 0.7729503093850241 2313 40-41 0.802363097762786 2313 42-43 0.6487884389204694 2313 44-45 0.744445554921576 2313 46-47 0.7777086354629787 2313 48-49 0.7491058488720981 2313 50-51 0.6305869733390566 2313 52-53 0.5825750101391733 2313 54-55 0.5112361978510052 2313 56-57 0.5218138630064502 2313 58-59 0.5324581661994685 2313 60-61 0.525954982815442 2313 62-63 0.5119824164101061 2313 64-65 0.5442260851269651 2313 66-67 0.5891406706191091 2313 68-69 0.5737036136346063 2313 70-71 0.5708732989683654 2313 72-73 0.6027827259375584 2313 74-75 0.5529024028785514 2313 76-77 0.5897511772842066 2313 78-79 0.5904994257549063 2313 80-81 0.6434717710797955 2313 82-83 0.6142298564999358 2313 84-85 0.6055327130796684 2313 86-87 0.5379517587378793 2313 88-89 0.6216760437192796 2313 90-91 0.7079428170683819 2313 92-93 0.7463778883741163 2313 94-95 0.7186918339562993 2313 96-97 0.7722398436835327 2313 98-99 0.887100816081535 2313 100 0.920681045231202 2314 1 0.33224372544645675 2314 2 0.20150313668696285 2314 3 0.3048856254412442 2314 4 0.2711938135823999 2314 5 0.24220521247058002 2314 6 0.2974032574651133 2314 7 0.380683793514784 2314 8 0.34633270939696814 2314 9 0.38816395384689883 2314 10-11 0.4258554105699446 2314 12-13 0.4759531448531007 2314 14-15 0.4690561888242044 2314 16-17 0.526273353723866 2314 18-19 0.5751515539746066 2314 20-21 0.6038364732962691 2314 22-23 0.6433945184338015 2314 24-25 0.6281127593769824 2314 26-27 0.6167369135790253 2314 28-29 0.631472960084686 2314 30-31 0.6566499657635916 2314 32-33 0.681656339982446 2314 34-35 0.7035167986338067 2314 36-37 0.6642369902295187 2314 38-39 0.696027726156089 2314 40-41 0.7562111594625307 2314 42-43 0.5571358491194829 2314 44-45 0.6954107829240144 2314 46-47 0.5680166777888473 2314 48-49 0.39969629280538044 2314 50-51 0.5343002137508108 2314 52-53 0.5254338461321382 2314 54-55 0.5188729331363291 2314 56-57 0.5331160743437025 2314 58-59 0.4589307175950097 2314 60-61 0.33370478941899506 2314 62-63 0.4415151528957466 2314 64-65 0.4820859936430253 2314 66-67 0.4808144826856733 2314 68-69 0.5111448530605571 2314 70-71 0.4420766812954682 2314 72-73 0.5003007192240148 2314 74-75 0.5366302877945444 2314 76-77 0.5601441200822208 2314 78-79 0.568098097880025 2314 80-81 0.5843843769914443 2314 82-83 0.5900413229128496 2314 84-85 0.6196816745578353 2314 86-87 0.6325047248347389 2314 88-89 0.5505398081840767 2314 90-91 0.5479575271790829 2314 92-93 0.7235790708878831 2314 94-95 0.7406570927652538 2314 96-97 0.785447494994866 2314 98-99 0.8393543554077603 2314 100 0.8956787062180567 2315 1 0.276791001069963 2315 2 0.3088749530371864 2315 3 0.3213215562243228 2315 4 0.21583379598133945 2315 5 0.23264079006147398 2315 6 0.28634284714143377 2315 7 0.24897133448599362 2315 8 0.24228013772476942 2315 9 0.15049733206698335 2315 10-11 0.2814553759490721 2315 12-13 0.3237081586725239 2315 14-15 0.4700258228671501 2315 16-17 0.28957335041555154 2315 18-19 0.45494497657966804 2315 20-21 0.32532319247314945 2315 22-23 0.24399484285989814 2315 24-25 0.45353344216159286 2315 26-27 0.524860958912619 2315 28-29 0.4478619493554632 2315 30-31 0.4871921027124273 2315 32-33 0.5334351840811493 2315 34-35 0.3781952814141505 2315 36-37 0.58576159538719 2315 38-39 0.5988680496073329 2315 40-41 0.6354745998111397 2315 42-43 0.6461325327316629 2315 44-45 0.6373057841859691 2315 46-47 0.6639590202056738 2315 48-49 0.5760496829918154 2315 50-51 0.5407857510353438 2315 52-53 0.31069047795549665 2315 54-55 0.4734270869077548 2315 56-57 0.4960178233962509 2315 58-59 0.49201062272337026 2315 60-61 0.588622705743127 2315 62-63 0.6026414904868105 2315 64-65 0.542552405186477 2315 66-67 0.4124715354404742 2315 68-69 0.48539840304052717 2315 70-71 0.4026023188366281 2315 72-73 0.4553021827460455 2315 74-75 0.1872209773819904 2315 76-77 0.41749763138528095 2315 78-79 0.49396012628522357 2315 80-81 0.566239675346786 2315 82-83 0.45253611443916597 2315 84-85 0.4983277383942699 2315 86-87 0.46516573271416206 2315 88-89 0.4085225907529839 2315 90-91 0.5886862967808248 2315 92-93 0.7271135541755491 2315 94-95 0.7364326255902611 2315 96-97 0.720056717155213 2315 98-99 0.7784119950033208 2315 100 0.7341662300058012 2316 1 0.16038182256187028 2316 2 0.15749773672722256 2316 3 0.1834102632457295 2316 4 0.08057064079807219 2316 5 0.07653573366459909 2316 6 -0.041696386570833965 2316 7 -0.05017762832433448 2316 8 0.040718225029607424 2316 9 0.07323596233128171 2316 10-11 0.14128267431673436 2316 12-13 0.11809454758880378 2316 14-15 -0.018009500808368273 2316 16-17 0.15892838537310183 2316 18-19 0.2333304633888318 2316 20-21 0.2821202192420529 2316 22-23 0.1928843574188832 2316 24-25 0.17727226072035762 2316 26-27 0.041137267384847576 2316 28-29 0.11945192354372836 2316 30-31 -0.47922544037758996 2316 32-33 -0.29401451238270226 2316 34-35 -0.399137672560812 2316 36-37 -0.1349745227417074 2316 38-39 -0.06518590972544303 2316 40-41 0.08179918375994788 2316 42-43 0.15071956264385022 2316 44-45 0.02123537748168758 2316 46-47 -0.13090242243620054 2316 48-49 -0.056660350301633855 2316 50-51 0.06332363133321905 2316 52-53 -0.21579248682148489 2316 54-55 -0.14205007995953878 2316 56-57 -0.1820401731083905 2316 58-59 -0.5685831209408931 2316 60-61 -0.08538124869027541 2316 62-63 -0.06095648710684998 2316 64-65 -0.40517109929391637 2316 66-67 -0.7463616750881954 2316 68-69 -0.7258405452808532 2316 70-71 -0.4597494783899734 2316 72-73 -0.683220690999029 2316 74-75 -0.45883637169858105 2316 76-77 -0.08301618556150459 2316 78-79 0.004218046885505089 2316 80-81 -0.09479975763440862 2316 82-83 0.10351267282193533 2316 84-85 0.09361053991676371 2316 86-87 0.16848210905187244 2316 88-89 0.2306165121888064 2316 90-91 -0.3529926737898066 2316 92-93 -0.3125955558445419 2316 94-95 0.021961519555148357 2316 96-97 0.2473506262370293 2316 98-99 0.41799061823330064 2316 100 0.2545342643863293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 280.0 1 685.0 2 450.0 3 376.0 4 379.0 5 355.0 6 351.0 7 804.0 8 2077.0 9 3702.0 10 5549.0 11 7498.0 12 10329.0 13 13362.0 14 16604.0 15 20423.0 16 26089.0 17 33806.0 18 44078.0 19 55841.0 20 74136.0 21 97464.0 22 107446.0 23 100673.0 24 90757.0 25 91290.0 26 98853.0 27 113953.0 28 134491.0 29 161767.0 30 197256.0 31 245715.0 32 313052.0 33 410439.0 34 588101.0 35 819966.0 36 1243085.0 37 1799486.0 38 1781633.0 39 509965.0 40 199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.75034464463291 22.464322732489933 13.931028992663741 21.85430363021342 2 14.714694993220528 21.04033175397121 42.413780863532494 21.831192389275767 3 17.501420114724013 29.71362592215277 29.73466465880483 23.050289304318387 4 11.305848271012586 18.08744204689431 42.08120717060659 28.52550251148651 5 11.56976717073658 39.98262855040795 35.585107577077544 12.862496701777934 6 26.77148527439221 40.996424025041044 18.98454894605555 13.247541754511191 7 25.747270751180874 33.55208111968492 24.497202816243508 16.203445312890693 8 25.402312602578746 35.65279483318233 20.81579870219957 18.129093862039365 9 26.604196944058284 15.796888654653943 22.847499130386492 34.75141527090128 10-11 24.083430087260762 27.61207944582127 28.218602916675344 20.085887550242624 12-13 24.826977402661974 25.778078330322984 28.6921773806213 20.702766886393743 14-15 22.88789440488864 26.67908385752534 26.544774286056715 23.8882474515293 16-17 20.17238567492874 29.624207711049312 28.078909693472642 22.124496920549312 18-19 19.719525631419856 28.958752126336307 31.08469523258655 20.23702700965729 20-21 21.268466692112746 28.013048556361614 30.67158723065263 20.04689752087301 22-23 21.77778937314556 27.808951176745715 30.10028323734882 20.31297621275991 24-25 21.021160346454927 28.20680052389009 30.901084659939627 19.870954469715358 26-27 20.727103220880842 28.440469095772364 30.19993627088325 20.632491412463544 28-29 20.08088396744767 27.953477669958527 31.14860484431588 20.817033518277924 30-31 20.859739642233308 28.0577343583589 30.767077384562985 20.315448614844804 32-33 21.01817921078368 28.5438683815398 30.046500391684155 20.391452015992364 34-35 21.07990567133779 28.24311185218878 30.660904861327992 20.016077615145438 36-37 20.877414506420035 27.827489708434435 30.17296490429838 21.122130880847152 38-39 20.9285014323861 28.154651595004516 30.745649483511684 20.171197489097707 40-41 20.574185726984425 27.215091168450172 31.632393073566117 20.578330030999286 42-43 20.549404774788012 27.30632038222085 30.75807168262915 21.386203160361983 44-45 20.4586327325278 28.314751868001437 30.393042474452294 20.83357292501847 46-47 20.785728552908342 28.005064365659393 29.731541315895317 21.477665765536948 48-49 21.276273491408034 27.969721428746958 29.66853634314412 21.085468736700886 50-51 21.248678263538398 27.749302699767924 29.217046525937118 21.78497251075656 52-53 20.591983008048846 28.501907265358945 29.254326894334888 21.65178283225732 54-55 20.912138297331648 28.536891650285913 28.959922828229946 21.591047224152497 56-57 21.37283311463547 28.1471689249775 28.60831673887584 21.871681221511192 58-59 20.474480284222548 28.139331622242477 29.688312380091514 21.69787571344347 60-61 20.758129685404842 29.028807437492095 28.696958636657943 21.516104240445124 62-63 21.035417465658497 28.201114312201614 28.9584256637893 21.80504255835059 64-65 20.283409936770973 28.141250837256806 29.130781519965822 22.4445577060064 66-67 20.981240582049672 27.878933841916208 28.667806855671163 22.472018720362954 68-69 20.38567422876817 28.07041374121415 29.046047974256922 22.497864055760758 70-71 20.731038497745896 27.482418989129982 29.297983365644342 22.488559147479776 72-73 21.14429936150245 27.77407385306419 28.987560961055447 22.09406582437791 74-75 21.145483388767573 28.131824749623803 28.735421323177075 21.987270538431556 76-77 20.734074710288766 28.022818675304816 28.991307279799226 22.251799334607185 78-79 20.614578242977803 27.795592515213563 28.78198949394018 22.807839747868453 80-81 20.338039486420588 28.437797064012766 28.917027110438497 22.307136339128157 82-83 21.454261495222866 27.482011664611473 29.583178988229125 21.480547851936535 84-85 21.078684684616263 27.682564102285912 28.729476337743794 22.50927487535403 86-87 20.148215935369322 28.293224856099318 29.40675111298922 22.15180809554213 88-89 21.130231193812843 27.198239432665574 29.20422332613923 22.467306047382355 90-91 20.899713646441654 27.343132573706026 29.356125226907025 22.4010285529453 92-93 21.20735984994133 27.547693472440276 29.06824089664092 22.176705780977475 94-95 20.02746423377403 27.039459991244957 29.774348151728365 23.158727623252652 96-97 20.79737156985841 27.04281751634251 29.259374039342244 22.900436874456837 98-99 20.221138580176913 27.421455244329508 29.227857112626303 23.129549062867273 100 20.99014396505553 26.838752167499287 28.395486498614243 23.77561736883094 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2817.0 1 1879.5 2 1263.0 3 1601.0 4 1401.0 5 1344.5 6 2021.0 7 3830.0 8 6395.0 9 7754.0 10 7077.5 11 6324.5 12 6872.5 13 7958.0 14 9057.5 15 10277.5 16 11607.5 17 12779.0 18 13790.5 19 14742.0 20 15856.5 21 17162.5 22 18616.0 23 20554.5 24 22290.5 25 24915.0 26 31123.0 27 38211.0 28 43391.0 29 57080.5 30 84065.5 31 112446.0 32 155929.5 33 216486.5 34 276086.5 35 329741.5 36 397184.5 37 470681.0 38 483174.5 39 454716.0 40 405028.5 41 347917.0 42 315036.5 43 309084.5 44 323109.5 45 355651.0 46 366833.0 47 355630.5 48 353084.5 49 337566.5 50 315098.0 51 299517.5 52 284033.0 53 263811.0 54 245178.0 55 207498.5 56 176920.0 57 140160.5 58 100722.5 59 92683.0 60 75642.0 61 49453.0 62 34085.0 63 27644.0 64 19795.0 65 8429.0 66 3796.0 67 2516.5 68 1878.0 69 1377.5 70 986.0 71 741.0 72 597.0 73 509.0 74 401.0 75 293.5 76 224.5 77 173.0 78 134.0 79 93.0 80 74.0 81 57.0 82 43.5 83 33.0 84 30.0 85 27.0 86 20.0 87 16.5 88 9.0 89 5.0 90 4.0 91 4.5 92 5.0 93 4.5 94 2.5 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02600087934583609 2 0.11272107659687741 3 0.01820495263622135 4 0.018779617609252758 5 0.02047108432232633 6 0.046623761962925434 7 0.047556237202183944 8 0.03933744381430081 9 0.034176301792358366 10-11 0.03796041642609348 12-13 0.037396594188402285 14-15 0.03274506072744996 16-17 0.03978741733091974 18-19 0.03862724464951671 20-21 0.0496651492258558 22-23 0.049057956046803755 24-25 0.0686128292328819 26-27 0.06102291449473124 28-29 0.052793278371507886 30-31 0.05116144670280551 32-33 0.045978619210182634 34-35 0.047252640612657916 36-37 0.04260652851937569 38-39 0.04776767054131814 40-41 0.05740686225876947 42-43 0.058241752879966045 44-45 0.06174937776252566 46-47 0.06306134873869171 48-49 0.06236199230924783 50-51 0.056929781903800006 52-53 0.06685630610776704 54-55 0.05298302623996166 56-57 0.057791779363347104 58-59 0.05484797671847868 60-61 0.051085547555423994 62-63 0.05876762554396649 64-65 0.055981042561531164 66-67 0.054159463024375014 68-69 0.05996032643139015 70-71 0.06045909225704005 72-73 0.06305050600335149 74-75 0.05351974163930231 76-77 0.05477749893876728 78-79 0.06493714195254893 80-81 0.06507267614430164 82-83 0.05481544851245803 84-85 0.06136988202561813 86-87 0.059309762310977246 88-89 0.06712195312360231 90-91 0.07276017550051422 92-93 0.077736991021673 94-95 0.08139641419899564 96-97 0.07670150979668244 98-99 0.08625395963141205 100 0.13299699168307985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9222765.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.600069307127875 #Duplication Level Percentage of deduplicated Percentage of total 1 72.472954713155 32.32298803099053 2 15.473938953246474 13.80277499538117 3 5.614765818591587 7.51256833957432 4 2.5556155631229007 4.55922524950624 5 1.3271844632475667 2.9596259522092394 6 0.7788242860325826 2.084137028107654 7 0.47111216301694875 1.4708144585390788 8 0.2948374897382593 1.0519817981332775 9 0.20200947346766246 0.8108672865618739 >10 0.6673226715850433 4.743160687922039 >50 0.04351195429075099 1.3755905145901615 >100 0.06638390980452585 6.852483089742549 >500 0.017135114803738517 5.422801543028194 >1k 0.01369981026448608 11.295424198582428 >5k 5.380590134087262E-4 1.5838883518726494 >10k+ 1.655566195103773E-4 2.151668475258766 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47824 0.5185429749104525 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36991 0.4010836229699011 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21350 0.23149239951359488 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 10396 0.11272107659687741 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 9592 0.10400351738334436 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0842735340215218E-5 0.0 12-13 0.0 0.0 0.0 2.1685470680430436E-5 0.0 14-15 0.0 0.0 0.0 2.1685470680430436E-5 0.0 16-17 0.0 0.0 0.0 3.252820602064565E-5 0.0 18-19 0.0 0.0 0.0 3.794957369075326E-5 0.0 20-21 0.0 0.0 0.0 1.5721966243312065E-4 0.0 22-23 0.0 0.0 0.0 5.96350443711837E-4 0.0 24-25 0.0 0.0 0.0 0.0014800333739393772 0.0 26-27 0.0 0.0 0.0 0.0032853488080852107 0.0 28-29 0.0 0.0 0.0 0.008012781416419045 0.0 30-31 0.0 0.0 0.0 0.025420793005134577 0.0 32-33 0.0 0.0 0.0 0.0637715479034758 0.0 34-35 0.0 0.0 0.0 0.1219861939450913 0.0 36-37 0.0 0.0 0.0 0.21648605380273706 0.0 38-39 0.0 0.0 0.0 0.35524595931914127 0.0 40-41 0.0 0.0 0.0 0.5083562250583205 0.0 42-43 0.0 0.0 0.0 0.6571185539260731 0.0 44-45 0.0 0.0 0.0 0.8348689357258913 0.0 46-47 0.0 0.0 0.0 1.0470178953925422 0.0 48-49 0.0 0.0 0.0 1.2785699299505082 0.0 50-51 0.0 0.0 0.0 1.524293419598136 0.0 52-53 0.0 0.0 0.0 1.7603235038516107 0.0 54-55 0.0 0.0 0.0 2.0062204772646814 0.0 56-57 0.0 0.0 0.0 2.298535200669214 0.0 58-59 0.0 0.0 0.0 2.636145450957495 0.0 60-61 0.0 0.0 0.0 2.9782554364119656 0.0 62-63 0.0 0.0 0.0 3.324154957867841 0.0 64-65 0.0 0.0 0.0 3.675356576905082 0.0 66-67 0.0 0.0 0.0 4.05664678651142 0.0 68-69 0.0 0.0 0.0 4.470866383345992 0.0 70-71 0.0 0.0 0.0 4.927388912110414 0.0 72-73 0.0 0.0 0.0 5.384670432348651 0.0 74-75 0.0 0.0 0.0 5.825156555544893 0.0 76-77 0.0 0.0 0.0 6.305397567866036 0.0 78-79 0.0 0.0 0.0 6.816263886155616 0.0 80-81 0.0 0.0 0.0 7.372263090298842 0.0 82-83 0.0 0.0 0.0 7.932659023622525 5.421367670107609E-6 84-85 0.0 5.421367670107609E-6 0.0 8.460440009042841 1.0842735340215218E-5 86-87 0.0 1.0842735340215218E-5 0.0 9.003758634205685 1.0842735340215218E-5 88 0.0 1.0842735340215218E-5 0.0 9.42259723629519 1.0842735340215218E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21710 0.0 51.86877 1 GTATCAA 42380 0.0 42.186993 1 CAACGCA 50170 0.0 34.44667 5 ATCAACG 50495 0.0 34.272972 3 GTACTAG 6620 0.0 33.750267 1 TATCAAC 54300 0.0 32.46535 2 TACCCCG 485 0.0 31.970675 5 AACGCAG 55330 0.0 31.921217 6 TAGGCAT 8475 0.0 31.546572 5 TACCTGG 2860 0.0 30.260227 2 TCAACGC 58025 0.0 30.027277 4 GCATCGT 6700 0.0 29.945415 8 ACGCAGA 59310 0.0 29.059652 7 CGCAGAG 59480 0.0 28.857317 8 ACCTGGG 2990 0.0 28.130682 3 TCAAGAC 1565 0.0 27.92332 3 AAGCTAA 4935 0.0 27.898525 9 CTGTGCG 7610 0.0 27.539179 9 GCAGAGT 62310 0.0 27.042906 9 CTAGGCA 9325 0.0 27.008928 4 >>END_MODULE