FastQCFastQC Report
Mon 6 Jun 2016
ERR1211328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211328_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9222765
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419850.45523224325893596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343220.3721443623468667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202950.22005331372966785No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG104040.11280781847959913No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC94870.10286503017262177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA226350.050.963961
GTATCAA428150.040.2721631
TACCTGG28450.034.63342
CAACGCA509850.032.810635
ATCAACG513000.032.6623233
ACCTGGG30700.031.6378213
GTACTAG69200.031.6039641
TAGGCAT85750.030.9093595
AACGCAG562850.030.3961646
TATCAAC560900.030.3737852
CTGTGCG80400.030.1644959
TACCCCG4200.030.1571395
ACTGTGC87150.029.0132948
TGAACCG9400.028.9451545
TCAACGC591450.028.5995674
ACGCAGA612500.027.3194967
TAGTACT89150.027.3160724
TATTCTC56450.027.2574755
CGCAGAG614600.027.1879868
CTAGGCA97850.026.469794
Produced by FastQC (version 0.11.5)