FastQCFastQC Report
Mon 6 Jun 2016
ERR1211327_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211327_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7684743
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293030.3813139879889282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235620.30660752090213034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145150.18888074721561932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133600.051.1904341
GTATCAA242050.041.138321
TACCTGG63700.034.1131672
CAACGCA292700.033.4249045
ATCAACG294650.033.139693
TATCAAC305300.032.429842
TCAACGC306500.032.0732774
AACGCAG313050.031.6571776
GTACATG313100.029.369361
TACATGG321300.028.0627942
ACGCAGA351550.027.8569837
ACCTGGG78150.027.3584653
CGCAGAG360450.027.0896978
ACATGGG323300.026.714633
GTACCTG84750.026.5288871
TAACGCC13850.025.7855244
CATGGGG209700.024.9183044
TCTAACG14650.024.7213732
GCAGAGT391050.024.4315599
CTAACGC15500.023.6467913
Produced by FastQC (version 0.11.5)