##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211327_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7684743 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.274482829159023 31.0 31.0 34.0 28.0 34.0 2 31.275959521352895 31.0 31.0 34.0 28.0 34.0 3 31.324679823385114 31.0 31.0 34.0 28.0 34.0 4 34.93573617230921 37.0 35.0 37.0 32.0 37.0 5 34.98310561589373 37.0 35.0 37.0 32.0 37.0 6 35.04589808663738 37.0 35.0 37.0 32.0 37.0 7 34.974283720353434 37.0 35.0 37.0 32.0 37.0 8 34.97529117109056 37.0 35.0 37.0 32.0 37.0 9 36.51685319860404 39.0 37.0 39.0 32.0 39.0 10-11 36.43999779823476 39.0 37.0 39.0 32.0 39.0 12-13 36.352088352206444 39.0 37.0 39.0 32.0 39.0 14-15 37.459098436993926 40.0 37.0 41.0 31.0 41.0 16-17 37.45703180965193 40.0 37.0 41.0 31.0 41.0 18-19 37.46053361836564 40.0 37.0 41.0 31.0 41.0 20-21 37.423437062241376 40.0 37.0 41.0 31.0 41.0 22-23 37.297222171255434 40.0 37.0 41.0 31.0 41.0 24-25 37.18541153555819 40.0 36.0 41.0 31.0 41.0 26-27 37.037830673062196 40.0 36.0 41.0 30.0 41.0 28-29 36.89377081315537 39.0 36.0 41.0 30.0 41.0 30-31 36.700180214224474 39.0 36.0 41.0 30.0 41.0 32-33 36.5610525296682 39.0 35.0 41.0 30.0 41.0 34-35 36.40596666667968 39.0 35.0 41.0 29.0 41.0 36-37 36.30166187730676 39.0 35.0 41.0 29.0 41.0 38-39 36.148839915661455 39.0 35.0 40.0 28.0 41.0 40-41 35.97678081101736 39.0 35.0 40.0 27.5 41.0 42-43 35.81852203775715 38.0 35.0 40.0 27.0 41.0 44-45 35.67262659271755 38.0 34.5 40.0 26.5 41.0 46-47 35.493843906035636 38.0 34.0 40.0 26.0 41.0 48-49 35.24620881921491 38.0 34.0 40.0 24.5 41.0 50-51 34.57424932753119 37.5 33.5 39.5 24.0 40.5 52-53 34.82119206849208 38.0 33.5 40.0 24.5 40.5 54-55 35.17891873547366 38.0 34.0 40.0 25.0 41.0 56-57 35.085358157065244 38.0 34.0 40.0 25.0 41.0 58-59 34.8634514517922 38.0 34.0 40.0 25.0 41.0 60-61 34.59802006391105 37.5 33.0 40.0 24.0 41.0 62-63 34.32063616180788 37.0 33.0 40.0 24.0 41.0 64-65 34.041508609982145 36.0 33.0 40.0 23.0 41.0 66-67 33.82198005840924 36.0 32.5 39.0 23.0 41.0 68-69 33.48364154793465 35.5 32.0 39.0 22.5 41.0 70-71 33.139021109749535 35.0 32.0 39.0 22.0 40.5 72-73 32.769939541244256 35.0 31.5 37.5 21.5 40.0 74-75 32.39721289313123 35.0 31.0 37.0 20.5 39.5 76-77 32.03902433692318 35.0 31.0 36.5 20.0 39.0 78-79 31.671275213757962 35.0 30.5 36.0 20.0 39.0 80-81 31.333291106807344 34.5 30.0 36.0 19.5 37.5 82-83 31.01698996309961 34.0 30.0 35.0 18.5 37.0 84-85 30.70460944497428 34.0 29.5 35.0 18.0 37.0 86-87 30.44208186272462 34.0 29.0 35.0 18.0 36.0 88-89 30.17482712173979 34.0 29.0 35.0 17.0 36.0 90-91 29.90390238164113 34.0 29.0 35.0 16.0 35.5 92-93 29.65863386713128 34.0 29.0 35.0 15.0 35.0 94-95 29.424992872240487 34.0 27.5 35.0 13.0 35.0 96-97 29.21329360005923 34.0 27.0 35.0 11.0 35.0 98-99 28.96843232102882 33.5 27.0 35.0 10.0 35.0 100 27.276213791404604 31.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5039875803772169 1101 2 -0.4495485850753269 1101 3 -0.4711105164341056 1101 4 -0.3218505119426851 1101 5 -0.39932407492262456 1101 6 -0.28453223153261575 1101 7 -0.4446962750985364 1101 8 -0.5123965278790266 1101 9 -0.5864694322575801 1101 10-11 -0.5722181983627408 1101 12-13 -0.5465784825446178 1101 14-15 -0.6124013060269817 1101 16-17 -0.6277785939006861 1101 18-19 -0.6847188567594813 1101 20-21 -0.7274843791796073 1101 22-23 -0.7535359431600526 1101 24-25 -0.7113516561137985 1101 26-27 -0.8036177615996962 1101 28-29 -0.7803495268044145 1101 30-31 -0.8177884333671841 1101 32-33 -0.8822380111702302 1101 34-35 -0.8452031138947831 1101 36-37 -0.8587306494732232 1101 38-39 -0.9020999250501589 1101 40-41 -0.9520142362193127 1101 42-43 -0.9979342082489921 1101 44-45 -1.114197951979591 1101 46-47 -1.0164169722348575 1101 48-49 -0.985641963682248 1101 50-51 -1.0074161841910794 1101 52-53 -0.8450491380576395 1101 54-55 -0.7896349109839704 1101 56-57 -0.7670763532406042 1101 58-59 -0.826991657828728 1101 60-61 -0.7589511391298416 1101 62-63 -0.7915344988868824 1101 64-65 -0.7713692110777046 1101 66-67 -0.8444753059420691 1101 68-69 -0.8031709184381839 1101 70-71 -0.7894419620315674 1101 72-73 -0.8708486178455459 1101 74-75 -0.9160660438126875 1101 76-77 -0.9873514723017216 1101 78-79 -0.8724954440946497 1101 80-81 -0.9337184232462334 1101 82-83 -1.047540625018435 1101 84-85 -0.9860489292643493 1101 86-87 -1.0880235634797586 1101 88-89 -1.1827509860636631 1101 90-91 -1.0786041212944184 1101 92-93 -1.1662066395589399 1101 94-95 -1.3216622438559433 1101 96-97 -1.321827579278846 1101 98-99 -1.2638802138882177 1101 100 -1.3099714082503162 1102 1 -0.41050861598009547 1102 2 -0.4540901386826093 1102 3 -0.41057854198920296 1102 4 -0.3349110906158046 1102 5 -0.332039669932108 1102 6 -0.3814269502737275 1102 7 -0.36761602759727907 1102 8 -0.3732634599627289 1102 9 -0.43292960822128634 1102 10-11 -0.4716555051340805 1102 12-13 -0.5076137921606971 1102 14-15 -0.6582649085725834 1102 16-17 -0.6110290779371823 1102 18-19 -0.5975446434407985 1102 20-21 -0.6992781647126307 1102 22-23 -0.6705432177471451 1102 24-25 -0.7310510361910403 1102 26-27 -0.662219850965478 1102 28-29 -0.7642112861713883 1102 30-31 -0.7305829155962726 1102 32-33 -0.7924750959551545 1102 34-35 -0.8169907288386753 1102 36-37 -0.8330061298568125 1102 38-39 -0.9011052346808626 1102 40-41 -0.8973195961005374 1102 42-43 -0.8954343585361642 1102 44-45 -0.9125376807953103 1102 46-47 -0.9422459317600129 1102 48-49 -0.9507164721485992 1102 50-51 -0.9147692388133137 1102 52-53 -0.8930578910920772 1102 54-55 -0.7321924451701065 1102 56-57 -0.7175672225317058 1102 58-59 -0.8035386460265244 1102 60-61 -0.8256666889026718 1102 62-63 -0.8040602358336741 1102 64-65 -0.8360242355841905 1102 66-67 -0.7972798666567513 1102 68-69 -0.8350051598156298 1102 70-71 -0.9101250544195167 1102 72-73 -0.9533382359905715 1102 74-75 -0.9951060661248476 1102 76-77 -0.9635874585067796 1102 78-79 -0.91685221887262 1102 80-81 -0.8922750050546107 1102 82-83 -0.9577018137069686 1102 84-85 -0.9647195246397686 1102 86-87 -1.0978767473188498 1102 88-89 -1.1287641361696252 1102 90-91 -1.199962710440598 1102 92-93 -1.1838072238169808 1102 94-95 -1.1931683251327954 1102 96-97 -1.1899058445374706 1102 98-99 -1.194731581467071 1102 100 -1.2645659343228388 1103 1 -0.4934210046364207 1103 2 -0.5827576738860323 1103 3 -0.555587613128175 1103 4 -0.4673870648377161 1103 5 -0.39989601670156816 1103 6 -0.4505969945473183 1103 7 -0.4582999270290742 1103 8 -0.505926030839305 1103 9 -0.724624721476637 1103 10-11 -0.6796023052478262 1103 12-13 -0.7455737370297584 1103 14-15 -0.9134363849333553 1103 16-17 -0.8084853920814012 1103 18-19 -0.8241227758948568 1103 20-21 -0.8556355607154629 1103 22-23 -0.8473207907743117 1103 24-25 -0.9149841824609055 1103 26-27 -0.8279750597546851 1103 28-29 -0.8899833638687298 1103 30-31 -0.8904034365860554 1103 32-33 -0.9447634465276025 1103 34-35 -1.121911512140045 1103 36-37 -1.0758401180938293 1103 38-39 -1.1328589281340626 1103 40-41 -1.143415784017769 1103 42-43 -1.1092020600945887 1103 44-45 -1.083066210957 1103 46-47 -1.171897916680308 1103 48-49 -1.1726571785005717 1103 50-51 -1.1188927201544843 1103 52-53 -0.9452183341314111 1103 54-55 -0.9554745337974211 1103 56-57 -0.955273401137255 1103 58-59 -0.9201898559028905 1103 60-61 -0.8859028186053663 1103 62-63 -0.9953179491545612 1103 64-65 -0.8750651119219555 1103 66-67 -0.8535608669548651 1103 68-69 -0.8154635690551615 1103 70-71 -0.8548832055747368 1103 72-73 -0.9774720624888786 1103 74-75 -0.9279924427666302 1103 76-77 -0.833902329663669 1103 78-79 -0.8800719953689864 1103 80-81 -0.9730481129885256 1103 82-83 -1.122389700005158 1103 84-85 -1.0520533074129972 1103 86-87 -1.1092619311813898 1103 88-89 -1.1879198790220613 1103 90-91 -1.1287868493826174 1103 92-93 -1.1035654106317416 1103 94-95 -1.2974153959608152 1103 96-97 -1.2698457510783498 1103 98-99 -1.2728066885008076 1103 100 -1.2199054060247896 1104 1 -0.36100306636155466 1104 2 -0.41476871429453865 1104 3 -0.40526190212892743 1104 4 -0.2293840703516281 1104 5 -0.16552004424175237 1104 6 -0.25328812634537456 1104 7 -0.2795348263158317 1104 8 -0.3109172405813112 1104 9 -0.36762344151676274 1104 10-11 -0.45469430632466157 1104 12-13 -0.4939159646007738 1104 14-15 -0.589376063223213 1104 16-17 -0.5588544608915313 1104 18-19 -0.6169375453414929 1104 20-21 -0.5748911894700015 1104 22-23 -0.5143618809901653 1104 24-25 -0.6728830885537818 1104 26-27 -0.694895475528206 1104 28-29 -0.6284213328850612 1104 30-31 -0.7504341131717851 1104 32-33 -0.8089913040048842 1104 34-35 -0.7285906323077569 1104 36-37 -0.7451634765096813 1104 38-39 -0.7527721704048318 1104 40-41 -0.7191101282510672 1104 42-43 -0.7103325642050891 1104 44-45 -0.751244170816669 1104 46-47 -0.8237368651208428 1104 48-49 -0.778692204659734 1104 50-51 -0.6537253138248786 1104 52-53 -0.5598381832134933 1104 54-55 -0.6136730431798583 1104 56-57 -0.6184603574956213 1104 58-59 -0.7391712103040646 1104 60-61 -0.6285138963563313 1104 62-63 -0.6635782656528306 1104 64-65 -0.7607799313454393 1104 66-67 -0.7806207813611081 1104 68-69 -0.6390278496522512 1104 70-71 -0.6999828180076406 1104 72-73 -0.7353675312367187 1104 74-75 -0.805192056245776 1104 76-77 -0.7303187750136537 1104 78-79 -0.6590611078578874 1104 80-81 -0.6828008254458275 1104 82-83 -0.8436382584417323 1104 84-85 -0.9221668341111666 1104 86-87 -0.9072642558148694 1104 88-89 -0.9392216176977755 1104 90-91 -0.8901909128787189 1104 92-93 -0.9125857150932895 1104 94-95 -0.9175433756206672 1104 96-97 -0.9841467692220931 1104 98-99 -1.0216399300677672 1104 100 -1.0572598852172845 1105 1 -0.5494259390206366 1105 2 -0.5430915040983955 1105 3 -0.4948726037441453 1105 4 -0.4153247657255861 1105 5 -0.37095167263609596 1105 6 -0.3807048751843425 1105 7 -0.3744572309519896 1105 8 -0.3913327971253864 1105 9 -0.5238554777318782 1105 10-11 -0.574422294275557 1105 12-13 -0.6030746514905871 1105 14-15 -0.5857135131743121 1105 16-17 -0.6751476575324986 1105 18-19 -0.7288852636586185 1105 20-21 -0.7116966414673342 1105 22-23 -0.7497137377458003 1105 24-25 -0.6739022004346893 1105 26-27 -0.6879760655674332 1105 28-29 -0.8155298648772416 1105 30-31 -0.8557114251962261 1105 32-33 -0.8432246827848999 1105 34-35 -0.9251423203811129 1105 36-37 -0.9177282069227957 1105 38-39 -0.933732239803291 1105 40-41 -1.0994065269408608 1105 42-43 -1.0605183897930601 1105 44-45 -1.0638036202889225 1105 46-47 -0.9977419423723504 1105 48-49 -1.026338714746231 1105 50-51 -0.9808814827607861 1105 52-53 -0.8969212090104364 1105 54-55 -0.8938388060228348 1105 56-57 -1.0123273128258887 1105 58-59 -0.9411947775208631 1105 60-61 -0.8938085465352472 1105 62-63 -0.9372940434593673 1105 64-65 -0.941283388271998 1105 66-67 -0.952949167054733 1105 68-69 -0.9045282665891037 1105 70-71 -0.8866517048171545 1105 72-73 -0.983603229930246 1105 74-75 -0.9329489900236965 1105 76-77 -0.9080918185061151 1105 78-79 -0.8265814672746039 1105 80-81 -0.9079197182501737 1105 82-83 -0.8952450157045817 1105 84-85 -0.8310504953648241 1105 86-87 -0.9174761795088138 1105 88-89 -1.0202066836587775 1105 90-91 -1.037817176834313 1105 92-93 -1.0354813055997774 1105 94-95 -0.9933774365962336 1105 96-97 -1.0713895208636757 1105 98-99 -1.1155642528467666 1105 100 -1.1258329288243623 1106 1 -0.39988976873620175 1106 2 -0.4169053654731272 1106 3 -0.4293002597147044 1106 4 -0.2838563448205562 1106 5 -0.27968789473698763 1106 6 -0.31644856598442317 1106 7 -0.3209186313109882 1106 8 -0.32497554496754333 1106 9 -0.47544583534185136 1106 10-11 -0.45101042852258644 1106 12-13 -0.45566915901800087 1106 14-15 -0.5817284926595647 1106 16-17 -0.6507811699470736 1106 18-19 -0.697383001229305 1106 20-21 -0.60480339221332 1106 22-23 -0.6393462213524188 1106 24-25 -0.635069311353881 1106 26-27 -0.6049784670394516 1106 28-29 -0.6559399134135688 1106 30-31 -0.7092216222007863 1106 32-33 -0.6954998081763648 1106 34-35 -0.7539616068414432 1106 36-37 -0.744291085047017 1106 38-39 -0.8076151324466849 1106 40-41 -0.7860660472409577 1106 42-43 -0.7881246342364108 1106 44-45 -0.8913485087165114 1106 46-47 -0.8139184750295456 1106 48-49 -0.8451312144338772 1106 50-51 -0.8223328412805557 1106 52-53 -0.7230139903960762 1106 54-55 -0.742771445786957 1106 56-57 -0.6443433131177798 1106 58-59 -0.6457543998336064 1106 60-61 -0.6947558631752102 1106 62-63 -0.6336802415423435 1106 64-65 -0.5983732816261949 1106 66-67 -0.640605160019021 1106 68-69 -0.7221599767894702 1106 70-71 -0.7715766899717309 1106 72-73 -0.6832721438837623 1106 74-75 -0.7095233380202615 1106 76-77 -0.6652986449484644 1106 78-79 -0.6704597019576326 1106 80-81 -0.8539823035065943 1106 82-83 -0.7340751063285751 1106 84-85 -0.6988842705502876 1106 86-87 -0.7132971193190301 1106 88-89 -0.7427099350895396 1106 90-91 -0.7714948423764341 1106 92-93 -0.7776829025884702 1106 94-95 -0.7206843421754989 1106 96-97 -0.6917598901227535 1106 98-99 -0.7237638153433501 1106 100 -0.9372236002375658 1107 1 -0.47567211036525237 1107 2 -0.5142663710129654 1107 3 -0.4936951600294215 1107 4 -0.3240974872868563 1107 5 -0.2638065106133709 1107 6 -0.3009160546092531 1107 7 -0.26381674915722897 1107 8 -0.32955514092184757 1107 9 -0.5125532077217869 1107 10-11 -0.4819376983153276 1107 12-13 -0.4917570015447694 1107 14-15 -0.632990778581302 1107 16-17 -0.6293174318066761 1107 18-19 -0.6517457886353881 1107 20-21 -0.6147343692738829 1107 22-23 -0.6965351217969484 1107 24-25 -0.7595424528522514 1107 26-27 -0.7279658229034638 1107 28-29 -0.6949846811453284 1107 30-31 -0.7320253289069427 1107 32-33 -0.6202804429051767 1107 34-35 -0.6589119978347782 1107 36-37 -0.6868921666793071 1107 38-39 -0.7122704764384551 1107 40-41 -0.7139943765428214 1107 42-43 -0.7982309252648889 1107 44-45 -0.7196144990197624 1107 46-47 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2311 22-23 0.8894074570520232 2311 24-25 0.9324408774190545 2311 26-27 0.932087004696804 2311 28-29 0.9611940256951854 2311 30-31 0.9500849082355529 2311 32-33 0.9974098631431048 2311 34-35 0.9643875025831363 2311 36-37 0.9973841206858864 2311 38-39 1.0336314287166672 2311 40-41 1.0653866701265642 2311 42-43 1.0800980236442896 2311 44-45 1.1644741800807736 2311 46-47 1.1810812208037547 2311 48-49 1.1699854404751449 2311 50-51 1.1381669575214488 2311 52-53 0.9843265567845592 2311 54-55 0.9985122467617344 2311 56-57 1.0002083406206665 2311 58-59 0.9343784473264236 2311 60-61 0.9817901265702531 2311 62-63 0.9391985219606198 2311 64-65 0.930896498201264 2311 66-67 0.9644723998927276 2311 68-69 0.9742902825735058 2311 70-71 0.9047930405268332 2311 72-73 0.9209586672226848 2311 74-75 0.9446108814931833 2311 76-77 0.9768135236802564 2311 78-79 0.9412666920769865 2311 80-81 0.9993329479766153 2311 82-83 1.0359805730577776 2311 84-85 1.0949700428841176 2311 86-87 1.0484242869660108 2311 88-89 1.0421944030222932 2311 90-91 1.0818000231208558 2311 92-93 1.0659536881916871 2311 94-95 1.1474603912478685 2311 96-97 1.168650244426054 2311 98-99 1.2075241154128555 2311 100 1.2411803302843971 2312 1 0.5649395361899572 2312 2 0.5498259855737366 2312 3 0.6113849320418225 2312 4 0.5267945641327216 2312 5 0.5276474594825373 2312 6 0.5928844835321883 2312 7 0.5835519152250228 2312 8 0.6014503663215862 2312 9 0.6718565938496752 2312 10-11 0.691060119059749 2312 12-13 0.7076983590252439 2312 14-15 0.8699993683081715 2312 16-17 0.8713221195557068 2312 18-19 0.8781932541998572 2312 20-21 0.8669550869541425 2312 22-23 0.8979620991613402 2312 24-25 0.8905970632398805 2312 26-27 1.0001239068437897 2312 28-29 0.9995735001552077 2312 30-31 0.9986014520819495 2312 32-33 0.9762294146538437 2312 34-35 0.9525803930053769 2312 36-37 0.9889741469882694 2312 38-39 1.0671427139370238 2312 40-41 0.9886643411833163 2312 42-43 1.016014062546219 2312 44-45 1.019897017512136 2312 46-47 1.1067117468567602 2312 48-49 1.0756380138830224 2312 50-51 1.0748702234495155 2312 52-53 0.9690623733036077 2312 54-55 0.926394230341387 2312 56-57 0.8500711297525427 2312 58-59 0.8446625189124859 2312 60-61 0.882158197335464 2312 62-63 0.8826454567779862 2312 64-65 0.8468338902718315 2312 66-67 0.8260446100706389 2312 68-69 0.942067631792284 2312 70-71 0.9485528880544436 2312 72-73 0.8892546595070385 2312 74-75 0.8446548862892769 2312 76-77 0.8630248165168339 2312 78-79 0.8936443989405234 2312 80-81 0.9081314345712848 2312 82-83 0.8939089930687487 2312 84-85 0.9854960006892313 2312 86-87 0.9814958085111698 2312 88-89 1.024883084163644 2312 90-91 1.0373979806001614 2312 92-93 1.0924486413916341 2312 94-95 1.1391652954554594 2312 96-97 1.1157591438671588 2312 98-99 1.234574318432582 2312 100 1.2746411646936089 2313 1 0.5083187080416636 2313 2 0.5304871694558955 2313 3 0.5551721027156304 2313 4 0.43631297328715135 2313 5 0.45096675972720845 2313 6 0.4286343503581662 2313 7 0.46947605197094333 2313 8 0.4837935346062636 2313 9 0.5518460706349799 2313 10-11 0.5942081389923501 2313 12-13 0.6162354002412087 2313 14-15 0.8065847619785345 2313 16-17 0.819076767703983 2313 18-19 0.7721576100777554 2313 20-21 0.7859987353572961 2313 22-23 0.7530926532742015 2313 24-25 0.80309379075333 2313 26-27 0.8208286328974239 2313 28-29 0.8499927372375211 2313 30-31 0.8795877317936629 2313 32-33 0.8328016953120283 2313 34-35 0.8470235794993641 2313 36-37 0.9379185322086627 2313 38-39 0.9578110239269435 2313 40-41 0.9865108880580706 2313 42-43 0.8268892833870041 2313 44-45 0.919319432446386 2313 46-47 0.935259907158688 2313 48-49 0.9110939880306645 2313 50-51 0.8548057834961114 2313 52-53 0.765691773028017 2313 54-55 0.7100809473713454 2313 56-57 0.6925043463591791 2313 58-59 0.7091652886887232 2313 60-61 0.6931445769997779 2313 62-63 0.7493462226583674 2313 64-65 0.7746953313653506 2313 66-67 0.8156634049523959 2313 68-69 0.7450659855217125 2313 70-71 0.7349428103207956 2313 72-73 0.7373197758063696 2313 74-75 0.6973092557107563 2313 76-77 0.7267971378224161 2313 78-79 0.7551912948420636 2313 80-81 0.7822150646549169 2313 82-83 0.7899373124593367 2313 84-85 0.7730096144254865 2313 86-87 0.8633792268746916 2313 88-89 0.8169331427320259 2313 90-91 0.9344595343581936 2313 92-93 0.9755855422382673 2313 94-95 0.9953806620399455 2313 96-97 1.1042474748496858 2313 98-99 1.0108229346336906 2313 100 1.1692427504287863 2314 1 0.3018168122820235 2314 2 0.1905177351390428 2314 3 0.30398574257783295 2314 4 0.24285950558601144 2314 5 0.23958854376247274 2314 6 0.321813347684035 2314 7 0.3400634547857777 2314 8 0.32342364993333916 2314 9 0.4184925621771285 2314 10-11 0.4896800059636419 2314 12-13 0.482430796691645 2314 14-15 0.5280688677863452 2314 16-17 0.5241898315405109 2314 18-19 0.5295036463493759 2314 20-21 0.5270556310559655 2314 22-23 0.6020818278049163 2314 24-25 0.6310745453103834 2314 26-27 0.6262001489471771 2314 28-29 0.6164367200435876 2314 30-31 0.6940088627608887 2314 32-33 0.7284898167086311 2314 34-35 0.72854172436341 2314 36-37 0.7083506493810532 2314 38-39 0.7369790993420011 2314 40-41 0.7709854646298666 2314 42-43 0.6650279360064673 2314 44-45 0.7854664233782955 2314 46-47 0.6457804401514906 2314 48-49 0.5325396835052629 2314 50-51 0.6999974157708451 2314 52-53 0.6909122490796662 2314 54-55 0.677707502860514 2314 56-57 0.6785961660852067 2314 58-59 0.634180373843634 2314 60-61 0.43102581202777657 2314 62-63 0.6167337989940549 2314 64-65 0.6658013161719225 2314 66-67 0.6495410971398599 2314 68-69 0.6899380305218514 2314 70-71 0.6444511137147586 2314 72-73 0.7091490203514113 2314 74-75 0.7699373945407402 2314 76-77 0.7188378664474016 2314 78-79 0.6944254271620593 2314 80-81 0.6927045589946133 2314 82-83 0.6662395225222895 2314 84-85 0.778371726502364 2314 86-87 0.8122357850649635 2314 88-89 0.7010975360021057 2314 90-91 0.7021487214152238 2314 92-93 0.8413190724453479 2314 94-95 0.897432132255954 2314 96-97 0.9987464648001811 2314 98-99 0.9619520395417531 2314 100 0.9489209682780064 2315 1 0.33515580362234587 2315 2 0.3227816502198628 2315 3 0.34982321641870584 2315 4 0.266856925974281 2315 5 0.2840060958889268 2315 6 0.2813996137052186 2315 7 0.2808669320613717 2315 8 0.2771675201968833 2315 9 0.1563759585267448 2315 10-11 0.3408913046923132 2315 12-13 0.31946677374247656 2315 14-15 0.476400204337601 2315 16-17 0.31350269863575875 2315 18-19 0.5057078084818514 2315 20-21 0.36233319475111614 2315 22-23 0.36068995442415286 2315 24-25 0.5101168479763984 2315 26-27 0.612213903580475 2315 28-29 0.510599378039764 2315 30-31 0.5337704610801097 2315 32-33 0.5775090497209305 2315 34-35 0.4288409761698091 2315 36-37 0.636059813905824 2315 38-39 0.6657786478733314 2315 40-41 0.712971190705062 2315 42-43 0.7219459115679001 2315 44-45 0.7173645638715556 2315 46-47 0.7652459800507856 2315 48-49 0.6572144350793963 2315 50-51 0.572878093896712 2315 52-53 0.3079789221917011 2315 54-55 0.5501140253523786 2315 56-57 0.5540713974729741 2315 58-59 0.6269708278153843 2315 60-61 0.7046988857922614 2315 62-63 0.7204027876408716 2315 64-65 0.7216397659732223 2315 66-67 0.6359744598252419 2315 68-69 0.5997697789723446 2315 70-71 0.4904128439809128 2315 72-73 0.6243414282948194 2315 74-75 0.3217380049096832 2315 76-77 0.5698943664994971 2315 78-79 0.6230532213065487 2315 80-81 0.6981460785485183 2315 82-83 0.6628634501789001 2315 84-85 0.6730376724597917 2315 86-87 0.6904373152171033 2315 88-89 0.5196599005143518 2315 90-91 0.695377570219545 2315 92-93 0.8166540987862057 2315 94-95 0.8193540345474837 2315 96-97 0.9187270115101427 2315 98-99 0.9345447269430949 2315 100 0.9140641662091902 2316 1 0.11855251776591302 2316 2 0.16199689286669283 2316 3 0.14173131893317503 2316 4 0.03505885817839527 2316 5 0.03462832612134292 2316 6 -0.10876981627365723 2316 7 -0.09690368371433067 2316 8 0.030420283672675907 2316 9 0.10629048100946648 2316 10-11 0.10175169201186662 2316 12-13 0.10707948651749177 2316 14-15 -0.017173829457945544 2316 16-17 0.13028654231263204 2316 18-19 0.19724218874544164 2316 20-21 0.3104331663317197 2316 22-23 0.14784714981161073 2316 24-25 0.15855556100970603 2316 26-27 0.10355809173332631 2316 28-29 0.07045849832473294 2316 30-31 -0.4842027491223604 2316 32-33 -0.302933709350782 2316 34-35 -0.37183005317879747 2316 36-37 -0.06727047707106237 2316 38-39 0.013632247713594836 2316 40-41 0.18170665248104712 2316 42-43 0.21246045537183988 2316 44-45 0.10693656728071943 2316 46-47 -0.003372822114307894 2316 48-49 0.10261205548360408 2316 50-51 0.18968178898570187 2316 52-53 -0.13732081644045735 2316 54-55 0.016452013801263377 2316 56-57 0.026032807626755528 2316 58-59 -0.40715349274661605 2316 60-61 0.06526585923188577 2316 62-63 0.10152543001153447 2316 64-65 -0.2333871464249171 2316 66-67 -0.4876843399726525 2316 68-69 -0.5023448526271039 2316 70-71 -0.30111729651638797 2316 72-73 -0.4481497902755649 2316 74-75 -0.26598880592550955 2316 76-77 0.025927099530875353 2316 78-79 0.07192737130247906 2316 80-81 0.04804946422303402 2316 82-83 0.24423881485151 2316 84-85 0.322843198173004 2316 86-87 0.37935811403220754 2316 88-89 0.38671577021328574 2316 90-91 -0.20711391226084075 2316 92-93 -0.24506861022354443 2316 94-95 0.15499484765492966 2316 96-97 0.38990454958461385 2316 98-99 0.42475641645009077 2316 100 0.2593077651981126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 211.0 1 576.0 2 323.0 3 301.0 4 307.0 5 287.0 6 331.0 7 873.0 8 2295.0 9 3908.0 10 6213.0 11 10280.0 12 15495.0 13 19977.0 14 23883.0 15 27434.0 16 32488.0 17 39165.0 18 47388.0 19 56355.0 20 67119.0 21 80307.0 22 88819.0 23 91441.0 24 91340.0 25 94894.0 26 103522.0 27 117139.0 28 135367.0 29 160459.0 30 192101.0 31 234888.0 32 292478.0 33 375631.0 34 511122.0 35 701525.0 36 1016347.0 37 1431338.0 38 1303327.0 39 307202.0 40 287.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.98239828478619 18.653991383841305 12.871613820737146 23.491996510635353 2 15.944560979873776 19.856794733472864 40.6595326314111 23.539111655242255 3 19.157399887549197 25.757194768955248 29.34988078132484 25.73552456217072 4 12.637505232096856 16.276597793877247 37.88897126043035 33.19692571359554 5 13.267622480608551 37.54749188321724 34.568754632699715 14.616131003474484 6 30.98709709588643 36.603022025769874 18.210853885081367 14.19902699326233 7 27.78258609933333 32.20329097497138 22.131228642459806 17.882894283235483 8 26.16365404194811 35.2145070465256 20.128515338148482 18.493323573377815 9 26.892340767519133 15.759637335793721 20.490720306451568 36.85730159023558 10-11 25.21301863074603 25.984872099000007 28.048320346851757 20.7537889234022 12-13 25.642444548541054 23.80643680234742 28.21158488519834 22.339533763913185 14-15 23.058324952086473 25.25314749907564 26.057890383379156 25.63063716545873 16-17 22.233318425815593 28.06360066476551 26.92259301246393 22.780487896954966 18-19 21.914223112540792 27.244334063687027 29.098765935928956 21.742676887843228 20-21 22.890753908769387 26.486741026175807 28.969809122748913 21.652695942305893 22-23 22.860268538142282 26.28463564121088 28.65834820648011 22.196747614166725 24-25 22.407138791186203 26.366169573896208 29.053014931273612 22.173676703643977 26-27 22.31368549968341 26.577067187760612 28.77386330893006 22.335384003625922 28-29 22.1073857239126 26.73467934369952 28.950287154895953 22.207647777491932 30-31 22.659091685802117 26.527109958192224 28.877517376509203 21.936280979496452 32-33 22.485989136560566 26.767138011089443 28.586945724852665 22.15992712749733 34-35 22.643987160190314 26.762650404070737 28.757171750257594 21.83619068548136 36-37 22.57646320544776 26.606205891869543 28.554538531881203 22.262792370801495 38-39 22.508070964602318 26.785141926340522 28.658446724343133 22.048340384714027 40-41 22.49631927496878 26.3802067872205 28.928291872771645 22.195182065039077 42-43 22.320805172601577 26.5750550053412 28.509559507423482 22.594580314633742 44-45 22.223851232847103 26.924711498022834 28.525433615574613 22.326003653555446 46-47 22.27389348494828 26.66702430231914 28.227257578853944 22.83182463387864 48-49 22.48617833873661 26.755022695447416 28.15658644533519 22.602212520480787 50-51 22.428820536443283 26.911519759454762 28.02307412580165 22.636585578300306 52-53 22.269371783207855 27.172431322256767 27.862428232339393 22.695768662195988 54-55 22.231241165003794 26.993394848613367 27.97017477791061 22.805189208472225 56-57 22.48759559433894 26.94622239243824 27.664221948843664 22.901960064379153 58-59 22.237494439734533 26.951714208767307 28.05289577453676 22.7578955769614 60-61 22.461428439563008 27.104388852312262 27.813712125127733 22.620470582996994 62-63 22.385224728562612 27.020367088952874 27.849458648573467 22.744949533911047 64-65 22.156419955689582 26.826532062362684 27.90626731435158 23.110780667596153 66-67 22.270746934831905 26.77941582949657 27.698487788346355 23.251349447325172 68-69 22.006930101672957 26.85899733244795 27.86840293544654 23.265669630432555 70-71 22.134668192666606 26.537431919179294 27.9440471028787 23.3838527852754 72-73 22.20778144450413 26.82922383145423 27.668329009020997 23.294665715020646 74-75 22.241938036316633 26.91408472718128 27.607618758214876 23.236358478287205 76-77 22.125223245599614 26.732400500083166 27.65496344907657 23.487412805240655 78-79 22.05509072106797 26.647166882337487 27.6848607940141 23.612881602580448 80-81 21.95430213776963 26.917671985477497 27.561635056299565 23.5663908204533 82-83 22.32729678289699 26.633925222931005 27.793314227828812 23.245463766343192 84-85 22.329371798069317 26.74504713693777 27.437506913042114 23.488074151950798 86-87 21.9237759778054 26.88400181943021 27.732880459177622 23.459341743586766 88-89 22.158125830624456 26.4264767512394 27.706340737980266 23.709056680155882 90-91 22.33082744951326 26.37537888736779 27.683672394419716 23.61012126869923 92-93 22.35261845065033 26.424685217559123 27.575579267472826 23.647117064317722 94-95 21.987071155267902 26.26044776012499 27.75197657862903 24.000504505978082 96-97 22.085994268976048 26.165486067572015 27.69394360510433 24.054576058347603 98-99 21.96734029729141 26.248620804981044 27.553868049456188 24.230170848271356 100 22.235176286383957 26.099601784134247 27.233610810603746 24.43161111887805 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3013.0 1 2167.5 2 2865.0 3 3887.5 4 2586.0 5 2046.5 6 2920.0 7 5142.5 8 7956.5 9 9233.0 10 8034.0 11 6585.5 12 6645.0 13 7216.5 14 7782.5 15 8404.5 16 9075.5 17 9724.0 18 10441.5 19 11083.5 20 12156.0 21 13562.0 22 15260.0 23 17814.5 24 20965.0 25 25012.5 26 30476.5 27 37678.0 28 46096.5 29 56749.0 30 71902.0 31 87347.5 32 105771.0 33 127354.5 34 147525.5 35 166797.5 36 189126.0 37 213912.0 38 226310.5 39 230297.5 40 230692.5 41 228727.0 42 232460.5 43 242269.0 44 255218.5 45 273101.5 46 288232.0 47 297689.0 48 307410.5 49 312805.0 50 310099.5 51 304537.5 52 296891.0 53 285633.0 54 273427.0 55 253431.5 56 229488.5 57 201871.5 58 174044.0 59 152024.0 60 127091.0 61 101855.5 62 82077.0 63 65046.5 64 48813.5 65 35415.0 66 25997.0 67 19677.5 68 15151.5 69 11317.5 70 8724.0 71 6664.0 72 5062.0 73 3946.5 74 3046.5 75 2268.5 76 1613.5 77 1073.5 78 719.0 79 479.0 80 324.0 81 232.0 82 149.0 83 102.5 84 81.0 85 70.0 86 70.0 87 48.0 88 33.0 89 31.0 90 21.5 91 17.5 92 16.0 93 10.0 94 8.0 95 5.5 96 5.5 97 5.5 98 3.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025622197124874572 2 0.11046563300815655 3 0.017996698132910886 4 0.018204902883544708 5 0.020664321500406715 6 0.04689812008026814 7 0.04704126084632889 8 0.03922056990064599 9 0.032336800332815295 10-11 0.037014900823618954 12-13 0.036130030633425216 14-15 0.03140638535342041 16-17 0.03821207813976343 18-19 0.03706695201127741 20-21 0.0479001054426934 22-23 0.0478090158642911 24-25 0.0666710649920238 26-27 0.05842745814661596 28-29 0.05037253685647002 30-31 0.04886955881283213 32-33 0.044009279165223875 34-35 0.04523898847365487 36-37 0.04064547116279621 38-39 0.04560985318572137 40-41 0.054536631869146444 42-43 0.05663819857085657 44-45 0.05924726435223663 46-47 0.0608153063804476 48-49 0.06088037036502067 50-51 0.054549644666061055 52-53 0.06410103760138758 54-55 0.05199263007233944 56-57 0.05603960991278433 58-59 0.05373634485889769 60-61 0.048928116398947896 62-63 0.05580537956832129 64-65 0.05408769037559226 66-67 0.05212926443994288 68-69 0.05830383657592713 70-71 0.05898700841394436 72-73 0.06137485664777599 74-75 0.05123788785129184 76-77 0.05231794999520479 78-79 0.06283879630067005 80-81 0.062454918791688935 82-83 0.05282544907487472 84-85 0.058733258874109386 86-87 0.05616973788193047 88-89 0.06380824967080878 90-91 0.06961846349318383 92-93 0.07377605210740294 94-95 0.07734806486046443 96-97 0.07367194973208603 98-99 0.08264427320471225 100 0.1270048978866307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7684743.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.26362331072167 #Duplication Level Percentage of deduplicated Percentage of total 1 73.81225692834452 43.005695333872715 2 15.47622054170107 18.03401367830648 3 5.166894759134147 9.031260297970018 4 2.1311705162425847 4.966788646770968 5 1.0614089727715952 3.0920766284092127 6 0.5971755139653839 2.0876165517639445 7 0.3767047549216467 1.5363728758078783 8 0.2588686743810087 1.2066101544864765 9 0.18947884137457896 0.9935751455280407 >10 0.8527052659604267 8.521877684146212 >50 0.04720432576284212 1.898202615320574 >100 0.027424364784681337 2.9581542941569414 >500 0.0016776739517175008 0.656458911020625 >1k 6.590765735730055E-4 0.6378271427414239 >5k 5.991605211010456E-5 0.19737697245970803 >10k+ 8.987407816515684E-5 1.1760930672386918 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29303 0.3813139879889282 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23562 0.30660752090213034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14515 0.18888074721561932 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.90383907438414E-5 2 0.0 0.0 0.0 0.0 3.90383907438414E-5 3 0.0 0.0 0.0 0.0 3.90383907438414E-5 4 0.0 0.0 0.0 0.0 3.90383907438414E-5 5 0.0 0.0 0.0 0.0 3.90383907438414E-5 6 0.0 0.0 0.0 0.0 5.20511876584552E-5 7 0.0 0.0 0.0 0.0 5.20511876584552E-5 8 0.0 0.0 0.0 0.0 5.20511876584552E-5 9 0.0 0.0 0.0 0.0 5.20511876584552E-5 10-11 0.0 0.0 0.0 0.0 5.20511876584552E-5 12-13 0.0 0.0 0.0 6.5063984573069E-6 5.85575861157621E-5 14-15 0.0 0.0 0.0 2.60255938292276E-5 8.458317994498971E-5 16-17 0.0 0.0 0.0 3.90383907438414E-5 1.041023753169104E-4 18-19 0.0 0.0 0.0 6.5063984573069E-5 1.041023753169104E-4 20-21 0.0 0.0 0.0 1.95191953719207E-4 1.041023753169104E-4 22-23 0.0 0.0 0.0 5.985886580722349E-4 1.236215706888311E-4 24-25 0.0 0.0 0.0 0.0014574332544367457 1.431407660607518E-4 26-27 0.0 0.0 0.0 0.0031490968533365396 1.6265996143267252E-4 28-29 0.0 0.0 0.0 0.0077230949688232905 1.8217915680459321E-4 30-31 0.0 0.0 0.0 0.02432091743341319 1.8217915680459321E-4 32-33 0.0 0.0 0.0 0.06115363910022755 1.8217915680459321E-4 34-35 0.0 0.0 0.0 0.1163604300104766 1.8217915680459321E-4 36-37 0.0 0.0 0.0 0.2031948238216945 1.886855552619001E-4 38-39 0.0 0.0 0.0 0.3276231879192317 1.95191953719207E-4 40-41 0.0 0.0 0.0 0.46386717161523816 1.95191953719207E-4 42-43 0.0 0.0 0.0 0.594756389380881 1.95191953719207E-4 44-45 0.0 0.0 0.0 0.7556921552223672 2.016983521765139E-4 46-47 0.0 0.0 0.0 0.9445859152349012 2.147111490911277E-4 48-49 0.0 0.0 0.0 1.1438638871852969 2.212175475484346E-4 50-51 0.0 0.0 0.0 1.3455557329633534 2.212175475484346E-4 52-53 0.0 0.0 0.0 1.5449508200859807 2.407367429203553E-4 54-55 0.0 0.0 0.0 1.7713799927987184 2.732687352068898E-4 56-57 0.0 0.0 0.0 2.028213565502451 2.797751336641967E-4 58-59 0.0 0.0 0.0 2.312686318852823 2.862815321215036E-4 60-61 0.0 0.0 0.0 2.6008026553392876 2.862815321215036E-4 62-63 0.0 0.0 0.0 2.8911702056919797 2.992943290361174E-4 64-65 0.0 0.0 0.0 3.1863329717077074 2.992943290361174E-4 66-67 0.0 0.0 0.0 3.5183805105779076 2.992943290361174E-4 68-69 0.0 0.0 0.0 3.8881391349066585 2.992943290361174E-4 70-71 0.0 0.0 0.0 4.27589445736832 2.992943290361174E-4 72-73 0.0 0.0 0.0 4.6555818457429226 3.6435831360918643E-4 74-75 0.0 0.0 0.0 5.030168217726995 3.6435831360918643E-4 76-77 0.0 0.0 0.0 5.441216446665816 3.708647120664933E-4 78-79 0.0 0.0 0.0 5.88817999508897 3.773711105238002E-4 80-81 0.0 0.0 0.0 6.3510647005371546 3.773711105238002E-4 82-83 0.0 0.0 0.0 6.818907281609809 4.424350950968692E-4 84-85 0.0 0.0 0.0 7.282045736597828 4.424350950968692E-4 86-87 0.0 1.30127969146138E-5 0.0 7.762836050600521 4.424350950968692E-4 88 0.0 1.30127969146138E-5 0.0 8.143395296368402 4.424350950968692E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13360 0.0 51.190434 1 GTATCAA 24205 0.0 41.13832 1 TACCTGG 6370 0.0 34.113167 2 CAACGCA 29270 0.0 33.424904 5 ATCAACG 29465 0.0 33.13969 3 TATCAAC 30530 0.0 32.42984 2 TCAACGC 30650 0.0 32.073277 4 AACGCAG 31305 0.0 31.657177 6 GTACATG 31310 0.0 29.36936 1 TACATGG 32130 0.0 28.062794 2 ACGCAGA 35155 0.0 27.856983 7 ACCTGGG 7815 0.0 27.358465 3 CGCAGAG 36045 0.0 27.089697 8 ACATGGG 32330 0.0 26.71463 3 GTACCTG 8475 0.0 26.528887 1 TAACGCC 1385 0.0 25.785524 4 CATGGGG 20970 0.0 24.918304 4 TCTAACG 1465 0.0 24.721373 2 GCAGAGT 39105 0.0 24.431559 9 CTAACGC 1550 0.0 23.646791 3 >>END_MODULE