FastQCFastQC Report
Mon 6 Jun 2016
ERR1211327_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211327_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7684743
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256860.3342467015487701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223870.29131748452745915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142210.18505498492272288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131550.053.0068551
GTATCAA231950.040.2185251
TACCTGG64400.032.7823642
ATCAACG295000.030.903243
CAACGCA295900.030.8969445
TATCAAC303250.030.5084232
TCAACGC309850.029.588924
AACGCAG316400.029.1915996
GTACATG309350.028.2444841
TACATGG316700.027.153642
ACCTGGG76100.026.9432533
GTACCTG80650.026.7644021
ACATGGG321100.025.8195953
ACGCAGA358700.025.487617
GTGGTAT61850.025.264381
CGCAGAG367800.024.8059888
CATGGGG207900.023.4034734
GTACTAG28450.022.5965631
GCAGAGT402500.022.5033959
TGGTATC66850.022.4575422
Produced by FastQC (version 0.11.5)