##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211327_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7684743 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09602038740918 33.0 31.0 34.0 30.0 34.0 2 32.21934591696821 33.0 31.0 34.0 30.0 34.0 3 32.285805263754426 34.0 31.0 34.0 30.0 34.0 4 35.932255119006584 37.0 35.0 37.0 35.0 37.0 5 35.83827201508235 37.0 35.0 37.0 35.0 37.0 6 35.853971043664046 37.0 35.0 37.0 35.0 37.0 7 35.76836805082486 37.0 35.0 37.0 33.0 37.0 8 35.750378509730254 37.0 35.0 37.0 33.0 37.0 9 37.36687902249952 39.0 37.0 39.0 34.0 39.0 10-11 37.313616798896206 39.0 37.0 39.0 34.0 39.0 12-13 37.24158732439068 39.0 37.0 39.0 33.0 39.0 14-15 38.46811838730326 40.0 38.0 41.0 34.0 41.0 16-17 38.45054759801336 40.0 38.0 41.0 34.0 41.0 18-19 38.461089785305774 40.0 38.0 41.0 34.0 41.0 20-21 38.42941097704894 40.0 38.0 41.0 34.0 41.0 22-23 38.31613854881029 40.0 38.0 41.0 34.0 41.0 24-25 38.23129361645536 40.0 38.0 41.0 33.5 41.0 26-27 38.1291315142224 40.0 37.5 41.0 33.0 41.0 28-29 38.01832201805578 40.0 37.0 41.0 33.0 41.0 30-31 38.018431650869786 40.0 37.0 41.0 33.0 41.0 32-33 37.94606163146901 40.0 37.0 41.0 33.0 41.0 34-35 37.808145308177515 40.0 37.0 41.0 33.0 41.0 36-37 37.658467108138815 40.0 37.0 41.0 33.0 41.0 38-39 37.53887495001459 40.0 36.5 41.0 32.0 41.0 40-41 37.42746777608568 40.0 36.0 41.0 32.0 41.0 42-43 37.31763072623248 40.0 36.0 41.0 31.5 41.0 44-45 37.15372921384619 39.5 36.0 41.0 31.0 41.0 46-47 36.907577585873725 39.0 36.0 41.0 31.0 41.0 48-49 35.34527869051704 39.0 35.0 40.5 24.0 41.0 50-51 36.07908488286466 39.0 35.0 40.0 28.0 41.0 52-53 36.1776785508637 39.0 35.0 40.0 29.0 41.0 54-55 36.00925665308521 38.5 35.0 40.0 29.0 41.0 56-57 35.79441550615291 38.0 35.0 40.0 28.0 41.0 58-59 35.5604380003339 38.0 34.0 40.0 28.0 41.0 60-61 35.30531274760912 37.5 34.0 40.0 28.0 41.0 62-63 34.98259837706999 37.0 34.0 40.0 27.0 41.0 64-65 34.65140213537395 36.5 33.5 39.0 26.0 41.0 66-67 34.31495705191442 36.0 33.0 39.0 26.0 40.5 68-69 33.95564783363608 35.5 33.0 39.0 26.0 40.0 70-71 33.598006920986165 35.0 32.5 38.0 26.0 40.0 72-73 33.218841280703856 35.0 32.0 37.5 25.0 39.5 74-75 32.85154701985479 35.0 32.0 37.0 25.0 39.0 76-77 31.91008801465449 34.0 30.5 36.0 23.5 38.5 78-79 32.343444016280046 34.5 31.5 36.0 25.0 38.0 80-81 32.21562334615484 35.0 31.5 35.5 25.0 37.0 82-83 31.943222759694113 34.0 31.0 35.0 24.0 37.0 84-85 31.65947364798016 34.0 31.0 35.0 24.0 36.5 86-87 31.395684735325567 34.0 31.0 35.0 23.0 36.0 88-89 31.16594113817469 34.0 31.0 35.0 23.0 36.0 90-91 30.944873498046714 34.0 30.0 35.0 20.5 35.0 92-93 30.747274632346198 34.0 30.0 35.0 20.0 35.0 94-95 30.548261340685045 34.0 30.0 35.0 20.0 35.0 96-97 30.35922287837082 34.0 29.5 35.0 19.5 35.0 98-99 30.13906053852419 34.0 29.0 35.0 19.0 35.0 100 27.719401546675016 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.27452233964099904 1101 2 -0.300983209481096 1101 3 -0.28408943934396547 1101 4 -0.18845423311454823 1101 5 -0.16958609258239932 1101 6 -0.225015015707271 1101 7 -0.28453477624491796 1101 8 -0.2760365491223524 1101 9 -0.3446629888167294 1101 10-11 -0.2687722980268532 1101 12-13 -0.4000013703405969 1101 14-15 -0.4733432793662331 1101 16-17 -0.47272641014929206 1101 18-19 -0.5209246706902135 1101 20-21 -0.5850555739695764 1101 22-23 -0.6958541509121403 1101 24-25 -0.5601514658028961 1101 26-27 -0.598328014458076 1101 28-29 -0.633424581611358 1101 30-31 -0.5326424498775424 1101 32-33 -0.4600954099943593 1101 34-35 -0.569066068523469 1101 36-37 -0.5780548362841103 1101 38-39 -0.49122803251953684 1101 40-41 -0.5511799591910105 1101 42-43 -0.5899101925052932 1101 44-45 -0.5286262491644109 1101 46-47 -0.542853415961865 1101 48-49 0.6356920489574307 1101 50-51 -0.2897219030113334 1101 52-53 -0.6354843301662711 1101 54-55 -0.6394308192264901 1101 56-57 -0.5686228258514561 1101 58-59 -0.6295522952770725 1101 60-61 -0.6314098745802283 1101 62-63 -0.6633612049460922 1101 64-65 -0.7734935874202478 1101 66-67 -0.7060156282312136 1101 68-69 -0.7091992810469421 1101 70-71 -0.6650495465443953 1101 72-73 -0.8174955519267613 1101 74-75 -0.9575430205056747 1101 76-77 -0.827908785461652 1101 78-79 -0.7356258598799741 1101 80-81 -0.569719715571452 1101 82-83 -0.6319905179571101 1101 84-85 -0.6597459605058447 1101 86-87 -0.6863369774547188 1101 88-89 -0.6972717756798801 1101 90-91 -0.7427008538099074 1101 92-93 -0.8497679129249391 1101 94-95 -0.7775564053128257 1101 96-97 -0.9123362908418713 1101 98-99 -1.1976727692723514 1101 100 -0.8323567958453602 1102 1 -0.2264700054769797 1102 2 -0.22031296911880105 1102 3 -0.2751399350594248 1102 4 -0.1625464878512659 1102 5 -0.15048986071823123 1102 6 -0.27791992697162016 1102 7 -0.21829666236912715 1102 8 -0.24276284045036078 1102 9 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2311 6 0.37130930453331956 2311 7 0.3944865777949005 2311 8 0.1936373815200625 2311 9 0.44502907360871546 2311 10-11 0.34158680412553366 2311 12-13 0.4462474345916334 2311 14-15 0.41088448858910454 2311 16-17 0.43264289045529125 2311 18-19 0.5866349252478145 2311 20-21 0.5985012598989101 2311 22-23 0.6467523496618526 2311 24-25 0.6120911307235772 2311 26-27 0.6450567262733813 2311 28-29 0.7531575407965576 2311 30-31 0.6148511953487699 2311 32-33 0.6194833314901516 2311 34-35 0.7400220079370783 2311 36-37 0.7899469894631821 2311 38-39 0.7847092965996296 2311 40-41 0.7231884084700795 2311 42-43 0.8015179009733941 2311 44-45 0.7844641386389597 2311 46-47 0.8427251130661588 2311 48-49 -5.855207663739762 2311 50-51 -1.0489416633031041 2311 52-53 0.37678325837956805 2311 54-55 0.7845483253036534 2311 56-57 0.9315644757645956 2311 58-59 0.963619911251179 2311 60-61 0.7571633516851222 2311 62-63 0.6319386040052493 2311 64-65 0.7437491084418042 2311 66-67 0.8232679582073601 2311 68-69 0.9168122693151233 2311 70-71 0.8793739638477263 2311 72-73 0.42616325700322477 2311 74-75 0.7262677779333941 2311 76-77 0.7504095261255266 2311 78-79 0.6476525944909355 2311 80-81 0.7450690571301024 2311 82-83 0.7591295507750004 2311 84-85 0.7922839619367252 2311 86-87 0.8028530652825516 2311 88-89 0.8595452665743615 2311 90-91 0.926739229404923 2311 92-93 0.8782226183000823 2311 94-95 0.9780178745853583 2311 96-97 1.0048411374939334 2311 98-99 1.0235708323679447 2311 100 1.2492402823102466 2312 1 0.11140910748925847 2312 2 0.2447837072223109 2312 3 0.24157254599008127 2312 4 0.041927553778322135 2312 5 0.23341656048094706 2312 6 0.20334073675720532 2312 7 0.3404271617999015 2312 8 0.39059240301206444 2312 9 0.2361007279087346 2312 10-11 0.24383678072154424 2312 12-13 0.32557951371776284 2312 14-15 0.3549864972070651 2312 16-17 0.4585185369740685 2312 18-19 0.34315635537195277 2312 20-21 0.39145378866731306 2312 22-23 0.6177862719648175 2312 24-25 0.7598224970460521 2312 26-27 0.6448702414050516 2312 28-29 0.8008621908658711 2312 30-31 0.8018854823309098 2312 32-33 0.8032903334359887 2312 34-35 0.41276623323317096 2312 36-37 0.725242544872998 2312 38-39 0.8097132766007178 2312 40-41 0.5765113788149776 2312 42-43 0.6328294752925814 2312 44-45 0.7840017180472572 2312 46-47 0.8899277673169834 2312 48-49 -4.776543378550603 2312 50-51 -0.7256650831104494 2312 52-53 0.6055987241084537 2312 54-55 0.8953729696290722 2312 56-57 0.9912123884721993 2312 58-59 0.9628392360208622 2312 60-61 0.9958531802832624 2312 62-63 0.8141846323343955 2312 64-65 1.0177985757563448 2312 66-67 1.0042140401673478 2312 68-69 0.8703628996891126 2312 70-71 0.9164197478762048 2312 72-73 0.9882986373353049 2312 74-75 0.6613008251650285 2312 76-77 0.9327577254460984 2312 78-79 0.5775545608494781 2312 80-81 0.7834301654057043 2312 82-83 0.7964671045843517 2312 84-85 0.7817746765016267 2312 86-87 0.9715192108908219 2312 88-89 0.9831789626122891 2312 90-91 0.783883747872391 2312 92-93 0.9310979971935431 2312 94-95 1.0379691202331394 2312 96-97 1.1181396235596992 2312 98-99 1.171072039590868 2312 100 0.679226324538984 2313 1 0.04768891530859776 2313 2 0.23632816581816485 2313 3 0.31901077111895404 2313 4 0.1295587655921011 2313 5 0.16552295002774997 2313 6 0.3054210322683488 2313 7 0.20474200787482033 2313 8 0.24979563471026722 2313 9 0.4326356147617503 2313 10-11 0.2278206709254036 2313 12-13 0.004149458560583241 2313 14-15 0.14349659991604824 2313 16-17 0.1116460089588287 2313 18-19 0.4621697937426532 2313 20-21 0.42671709136516967 2313 22-23 0.525992495705637 2313 24-25 0.33707049828239377 2313 26-27 0.46442532675624193 2313 28-29 0.4533621655573583 2313 30-31 0.6227316861632204 2313 32-33 0.555045385751562 2313 34-35 0.44227186255075424 2313 36-37 -0.22247665854579424 2313 38-39 0.2783231883874251 2313 40-41 0.4880160831865865 2313 42-43 0.5464841985206306 2313 44-45 0.6017110376916932 2313 46-47 0.4548724685413603 2313 48-49 -5.3016288091906105 2313 50-51 -1.2735066900201986 2313 52-53 0.20183161955969808 2313 54-55 0.39763050160205893 2313 56-57 0.37394051522337435 2313 58-59 0.5255451976423942 2313 60-61 0.8352906893414698 2313 62-63 0.8676659493354677 2313 64-65 0.9740549357210213 2313 66-67 1.051038353867817 2313 68-69 1.0079278800913514 2313 70-71 0.940496394092321 2313 72-73 0.7318561925408531 2313 74-75 0.8502661414848944 2313 76-77 0.9222973911866639 2313 78-79 0.8023731693068328 2313 80-81 -0.1255585013791105 2313 82-83 0.34675488477679295 2313 84-85 0.6757690501954343 2313 86-87 0.8337798580531519 2313 88-89 0.8409994269843821 2313 90-91 0.9161215879018414 2313 92-93 0.9365207652936469 2313 94-95 0.8636545099438422 2313 96-97 0.8295976064983037 2313 98-99 1.0103252834691858 2313 100 1.0374987668769045 2314 1 0.03414933751557214 2314 2 0.16890049788889172 2314 3 0.18122413008134686 2314 4 0.02036145841696424 2314 5 0.19027912108472123 2314 6 0.27544874165639044 2314 7 0.08022538870837792 2314 8 0.25259805557740833 2314 9 0.40115070712206347 2314 10-11 0.26686149525290404 2314 12-13 0.18086984057250532 2314 14-15 0.23217739434298323 2314 16-17 0.4510725255018855 2314 18-19 0.5154315497248874 2314 20-21 0.5497642150612236 2314 22-23 0.28682917955072185 2314 24-25 0.3786556977223654 2314 26-27 0.4082569671897005 2314 28-29 0.5624902269754628 2314 30-31 0.48543434732126656 2314 32-33 0.45438572199625327 2314 34-35 0.35483710376728084 2314 36-37 0.4417646329873435 2314 38-39 0.45172058697878725 2314 40-41 0.2661996236105466 2314 42-43 0.2669778572129502 2314 44-45 0.4682908700312467 2314 46-47 0.6052080000764519 2314 48-49 -6.521666864796359 2314 50-51 -1.6454826661027013 2314 52-53 -0.7917768751284413 2314 54-55 -0.3522374917453277 2314 56-57 -0.1100737285623481 2314 58-59 0.35481170992871114 2314 60-61 0.5978345545332076 2314 62-63 0.43400756001246776 2314 64-65 0.5759462612673474 2314 66-67 0.6929566276161694 2314 68-69 0.6784174372473686 2314 70-71 0.49699187889206087 2314 72-73 0.682951007127393 2314 74-75 0.701278371083724 2314 76-77 0.5472181410066206 2314 78-79 0.41016418531649634 2314 80-81 0.4591091245297392 2314 82-83 0.42023479445431633 2314 84-85 0.4288536456749483 2314 86-87 0.509321565688996 2314 88-89 0.4028468623971939 2314 90-91 0.5334262651682096 2314 92-93 0.7081262174547653 2314 94-95 0.8109681514688312 2314 96-97 0.7755259633748253 2314 98-99 0.8942922672104743 2314 100 -0.6271631353565112 2315 1 0.07316116751095336 2315 2 0.16713659132254577 2315 3 0.28188970579638095 2315 4 0.12474710963800106 2315 5 0.22677440140967065 2315 6 0.2839836163113958 2315 7 0.3401380796180504 2315 8 0.23927229555051355 2315 9 0.41684074501972646 2315 10-11 0.2053795222307926 2315 12-13 0.42147180857015343 2315 14-15 0.22857597218736458 2315 16-17 0.3257838343929933 2315 18-19 0.44273461949580195 2315 20-21 0.31644179490641733 2315 22-23 0.3969602896718385 2315 24-25 0.4178468993567037 2315 26-27 0.5209104314738511 2315 28-29 0.5751411094506054 2315 30-31 0.5537188560826607 2315 32-33 0.6297100915084215 2315 34-35 0.6359530385277168 2315 36-37 0.48713236407343885 2315 38-39 0.6217366289849977 2315 40-41 0.612140827579502 2315 42-43 0.7058055364760918 2315 44-45 0.7143394845794191 2315 46-47 0.5646417379481292 2315 48-49 -4.824495282347019 2315 50-51 -0.9419088931074313 2315 52-53 0.18720355567477753 2315 54-55 0.16085932957170002 2315 56-57 0.48211750767922723 2315 58-59 0.6050035969326331 2315 60-61 0.4748303066334074 2315 62-63 0.2547713286992277 2315 64-65 0.4440618354900536 2315 66-67 -0.2741471704446141 2315 68-69 0.5120733868431344 2315 70-71 0.5358811370467791 2315 72-73 0.6153246399359205 2315 74-75 0.7555018901919439 2315 76-77 0.6943283185160602 2315 78-79 0.5711774043617481 2315 80-81 0.4091072659934625 2315 82-83 0.4488559601246038 2315 84-85 0.5790395084578392 2315 86-87 0.5948205149293493 2315 88-89 0.6062125525724227 2315 90-91 0.6723684229416449 2315 92-93 0.7034634703770202 2315 94-95 0.31427543050397233 2315 96-97 0.4394135232119112 2315 98-99 0.36073369392912014 2315 100 0.4752292128207607 2316 1 0.21737123773985445 2316 2 0.27832341398837457 2316 3 0.20742082225176972 2316 4 -0.0303722706208589 2316 5 0.16106288295949156 2316 6 0.15973340607399678 2316 7 0.23491993667542488 2316 8 0.31643600556484586 2316 9 0.4517140162365436 2316 10-11 0.4110516085522917 2316 12-13 0.32971042814200047 2316 14-15 0.45322107927290745 2316 16-17 0.44421694957768665 2316 18-19 0.11833044721681318 2316 20-21 0.4426216440715436 2316 22-23 0.5875874156892493 2316 24-25 0.5980345975300381 2316 26-27 0.6169224532651114 2316 28-29 0.6743120297576084 2316 30-31 0.6168176836897388 2316 32-33 0.5803339660285829 2316 34-35 0.5102567577902875 2316 36-37 0.4130206435782213 2316 38-39 0.48355201305754036 2316 40-41 0.13673725187282315 2316 42-43 0.12678375706690304 2316 44-45 0.40786002994883575 2316 46-47 0.39001751828504183 2316 48-49 -5.109537901873594 2316 50-51 -0.9905247263455834 2316 52-53 0.33155801302867616 2316 54-55 0.5948514152282129 2316 56-57 0.4988577019988938 2316 58-59 0.38572232734764356 2316 60-61 0.5409576226829813 2316 62-63 0.44418838858332066 2316 64-65 0.4159953157744809 2316 66-67 0.5452562950450996 2316 68-69 0.25662145211261134 2316 70-71 -0.10017848838955956 2316 72-73 -0.0028987407114584585 2316 74-75 0.2509490427956891 2316 76-77 0.26134657922215965 2316 78-79 0.3167475937955757 2316 80-81 0.2331324038937126 2316 82-83 0.18296356759819332 2316 84-85 0.30485686427010705 2316 86-87 0.3627828379354625 2316 88-89 0.2914160251239757 2316 90-91 0.3164632476228846 2316 92-93 0.37378690214151433 2316 94-95 0.6044539955494379 2316 96-97 0.7069845554128804 2316 98-99 0.6515893129353643 2316 100 -0.010734070924392825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 1.0 9 1.0 10 8.0 11 15.0 12 51.0 13 183.0 14 680.0 15 2088.0 16 5254.0 17 10546.0 18 17869.0 19 25891.0 20 36088.0 21 49348.0 22 60768.0 23 64221.0 24 66265.0 25 72487.0 26 85230.0 27 103131.0 28 127136.0 29 155805.0 30 192081.0 31 236596.0 32 297365.0 33 386089.0 34 532887.0 35 749107.0 36 1150304.0 37 1698731.0 38 1374939.0 39 183548.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.06776508976939 19.383300051663497 13.685528506885927 23.863406351681185 2 15.668239945136955 20.29020416183557 40.2343781515995 23.807177741427974 3 18.74709326459796 25.697089477871593 29.949713314884647 25.606103942645802 4 12.614717050581842 16.693196984420478 37.80929513002285 32.88279083497483 5 13.156085948420642 37.68312681480687 34.62187603370957 14.538911203062915 6 31.08669417226716 36.91280041037183 17.904553154577293 14.095952262783715 7 27.779616197765467 32.2938749123425 21.96368673028453 17.9628221596075 8 26.247410845034334 35.525086548080104 19.88741689881276 18.340085708072802 9 26.695115372777607 15.731067327519959 20.379268363307936 37.1945489363945 10-11 25.232001215929195 26.14315432124631 27.92265446295723 20.70218999986727 12-13 25.551266461771334 23.83533254523431 28.251372054201205 22.362028938793152 14-15 22.898022271968802 25.33995677400121 25.98751393510791 25.774507018922076 16-17 22.2007235533244 28.130326524209394 26.898758164504116 22.77019175796209 18-19 21.86060377824005 27.269438583624538 29.09134750392401 21.7786101342114 20-21 22.817997728354122 26.492593467583436 28.983615768738456 21.705793035323985 22-23 22.699840794532165 26.340661443446862 28.69950363210856 22.259994129912418 24-25 22.32280322282841 26.39545863650178 29.077214773875575 22.20452336679423 26-27 22.238106522057944 26.614742963877617 28.800653815478654 22.34649669858578 28-29 22.05788621542496 26.79486098412656 28.97076670385763 22.176486096590846 30-31 22.611898132014254 26.559762765055112 28.932424529581102 21.895914573349536 32-33 22.483596591487697 26.7467551882988 28.621697235065476 22.14795098514803 34-35 22.58419096480604 26.754984807026496 28.800246091485306 21.860578136682165 36-37 22.549173370908584 26.61902382523863 28.622766859762194 22.209035944090587 38-39 22.472552262911012 26.73838813661384 28.769957028421402 22.019102572053747 40-41 22.448160408029118 26.382531725017593 29.000591917068885 22.168715949884398 42-43 22.291393301874503 26.573738578866156 28.602913800281065 22.531954318978272 44-45 22.134550489782054 26.90513962074588 28.603284994983103 22.35702489448896 46-47 22.198927211960783 26.665682346018293 28.299695834508153 22.835694607512767 48-49 22.533509655049734 26.812545027996965 28.13863291658788 22.51531240036542 50-51 22.381793873048174 26.857674991271708 28.074989449585612 22.685541686094503 52-53 22.214486845847137 27.12248554172255 27.927515662184877 22.735511950245435 54-55 22.185725067847077 26.945942342463574 28.010660061086607 22.857672528602745 56-57 22.45871136983935 26.902920843409518 27.70949488021962 22.928872906531513 58-59 22.131026629097285 26.933485786776828 28.0777520370162 22.85773554710968 60-61 22.393468442925165 27.12769823411973 27.821465030016512 22.657368292938592 62-63 22.318024540848143 27.010237152545336 27.809048422678945 22.862689883927576 64-65 22.017262916357144 26.834161272615397 27.885307388524293 23.26326842250316 66-67 22.143598621546953 26.857628188648913 27.679031308066033 23.3197418817381 68-69 21.90405051882702 26.908045300748896 27.831316330757012 23.356587849667076 70-71 22.01325013961542 26.68712081665438 27.823874422461394 23.475754621268806 72-73 22.129402577118313 26.930118443316413 27.539073278667185 23.40140570089809 74-75 22.158339902713525 27.10356542468237 27.38763594387434 23.350458728729766 76-77 22.052307574975313 26.934447251422473 27.43570778859833 23.577537385003883 78-79 21.997494958586262 26.862726814816224 27.46009635865832 23.6796818679392 80-81 21.915142749802882 27.173090244047486 27.31777054911238 23.59399645703725 82-83 22.246116493956134 26.904228379405925 27.534237616591405 23.315417510046537 84-85 22.301660624671023 27.00405003740779 27.141778122092564 23.552511215828623 86-87 21.871866451889005 27.195221283813687 27.39257949775764 23.540332766539667 88-89 22.008988127234414 26.77273851766388 27.401870976396157 23.816402378705547 90-91 22.164343031743886 26.736680493896447 27.355131340027377 23.74384513433229 92-93 22.138966222201574 26.87159576978917 27.221621060238903 23.767816947770356 94-95 21.75869563491666 26.726110062571014 27.408097806559383 24.107096495952945 96-97 21.863744524741477 26.64943881182457 27.413299148299984 24.073517515133965 98-99 21.717000683799572 26.73077819098441 27.259427883352693 24.29279324186332 100 21.72295375029761 26.62276066298734 27.218203240112594 24.43608234660246 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2913.0 1 2112.0 2 2565.0 3 3436.5 4 2358.5 5 1821.0 6 2621.0 7 4781.0 8 7159.5 9 8243.0 10 7321.0 11 5988.5 12 6050.0 13 6674.0 14 7399.5 15 8135.0 16 8885.0 17 9656.5 18 10442.5 19 11225.5 20 12328.5 21 13847.0 22 15601.0 23 17933.5 24 21200.5 25 25352.0 26 31073.5 27 38135.5 28 46116.5 29 57182.0 30 72254.0 31 87671.0 32 106138.0 33 127486.5 34 148328.5 35 167714.0 36 189414.0 37 214074.5 38 226937.5 39 230400.0 40 230668.0 41 228680.5 42 232414.0 43 243723.5 44 257447.0 45 275869.0 46 291569.5 47 300751.5 48 310739.0 49 316447.5 50 313389.0 51 306913.0 52 299346.5 53 288527.5 54 275491.0 55 254896.5 56 229786.5 57 200813.0 58 172938.0 59 150986.5 60 125489.0 61 99436.5 62 78863.0 63 61731.0 64 45630.0 65 32319.5 66 23476.5 67 17753.0 68 13317.5 69 9771.5 70 7530.5 71 5714.5 72 4274.5 73 3296.5 74 2557.0 75 1777.5 76 1110.5 77 677.0 78 419.5 79 249.0 80 162.5 81 100.0 82 53.0 83 33.5 84 22.0 85 14.0 86 10.5 87 8.5 88 10.0 89 7.5 90 3.5 91 2.5 92 1.5 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030397893592537837 2 0.0019909579279359115 3 6.5063984573069E-5 4 0.022238869927074984 5 5.335246734991658E-4 6 6.5063984573069E-5 7 5.2051187658455195E-5 8 0.003721659917579547 9 0.009603444122984986 10-11 0.001106087737742173 12-13 8.458317994498971E-5 14-15 2.0820475063382078E-4 16-17 3.838775089811071E-4 18-19 1.3012796914613799E-5 20-21 8.458317994498971E-5 22-23 2.537495398349691E-4 24-25 3.25319922865345E-5 26-27 7.80767814876828E-5 28-29 0.0010540365500837179 30-31 0.0027196745551542846 32-33 0.009701040099844589 34-35 0.018497690814123516 36-37 0.021757396441234277 38-39 0.021360506135338554 40-41 0.0050684843982420754 42-43 0.0053872979226501135 44-45 0.009278124200119638 46-47 0.01213443312287737 48-49 1.2093898260488347 50-51 0.018159358094343558 52-53 0.018582273994068508 54-55 0.009557899333783836 56-57 0.007488864624360242 58-59 0.008438798799127048 60-61 0.0050554716013274615 62-63 0.007482358225902935 64-65 0.019655829739524146 66-67 0.028862383556613407 68-69 0.028901421947357248 70-71 0.026435496932037936 72-73 0.022681305022171853 74-75 0.009570912130698451 76-77 0.014496255762879773 78-79 0.008308670829980911 80-81 0.0027847385397273532 82-83 0.014645902927397833 84-85 0.024086687088950145 86-87 0.022889509772805676 88-89 0.03249946029424797 90-91 0.020742398281894395 92-93 0.013175456876046474 94-95 0.006688577614111494 96-97 0.003754191909866081 98-99 0.015680420282109628 100 0.03547288438923722 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7684743.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.56545661578835 #Duplication Level Percentage of deduplicated Percentage of total 1 72.92670393884761 41.98059011724174 2 15.99014144140197 18.40959586850689 3 5.285514639250886 9.127891909737333 4 2.239491197058147 5.156693333827627 5 1.1083697178096403 3.190190445241222 6 0.641760681642263 2.2165948006077874 7 0.4007626881649513 1.6149060997150355 8 0.2721692637814739 1.2534038357090793 9 0.18555634052647071 0.9613471923324899 >10 0.8718711252100757 8.647990270495592 >50 0.04768431435951006 1.8876422597514515 >100 0.027372422570501136 2.979295305529797 >500 0.0018981026974251964 0.7528879319461604 >1k 5.816698653688768E-4 0.6320210367366138 >5k 3.061420343648282E-5 0.09112959821306062 >10k+ 9.184261030944847E-5 1.097819994408122 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25686 0.3342467015487701 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22387 0.29131748452745915 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14221 0.18505498492272288 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.30127969146138E-5 5 0.0 0.0 0.0 0.0 1.30127969146138E-5 6 0.0 0.0 0.0 0.0 1.30127969146138E-5 7 0.0 0.0 0.0 0.0 1.30127969146138E-5 8 0.0 0.0 0.0 0.0 1.30127969146138E-5 9 0.0 0.0 0.0 0.0 1.30127969146138E-5 10-11 0.0 0.0 0.0 0.0 1.30127969146138E-5 12-13 0.0 0.0 0.0 6.5063984573069E-6 1.30127969146138E-5 14-15 0.0 0.0 0.0 1.30127969146138E-5 2.60255938292276E-5 16-17 0.0 0.0 0.0 1.30127969146138E-5 2.60255938292276E-5 18-19 0.0 0.0 0.0 3.25319922865345E-5 2.60255938292276E-5 20-21 0.0 0.0 0.0 1.496471645180587E-4 2.60255938292276E-5 22-23 0.0 0.0 0.0 5.595502673283934E-4 3.25319922865345E-5 24-25 0.0 0.0 0.0 0.0014053820667782905 4.5544789201148303E-5 26-27 0.0 0.0 0.0 0.003136084056421926 5.20511876584552E-5 28-29 0.0 0.0 0.0 0.007736107765737904 5.20511876584552E-5 30-31 0.0 0.0 0.0 0.024516109387132397 5.85575861157621E-5 32-33 0.0 0.0 0.0 0.06146594622617829 6.5063984573069E-5 34-35 0.0 0.0 0.0 0.11718023621609727 6.5063984573069E-5 36-37 0.0 0.0 0.0 0.20371533569827904 6.5063984573069E-5 38-39 0.0 0.0 0.0 0.3233094457420371 6.5063984573069E-5 40-41 0.0 0.0 0.0 0.4541986635076801 1.171151722315242E-4 42-43 0.0 0.0 0.0 0.5797266089445021 1.171151722315242E-4 44-45 0.0 6.5063984573069E-6 0.0 0.7346244370176075 1.171151722315242E-4 46-47 6.5063984573069E-6 1.30127969146138E-5 0.0 0.9157170252798305 1.171151722315242E-4 48-49 1.30127969146138E-5 1.30127969146138E-5 0.0 1.1084430539837182 1.30127969146138E-4 50-51 1.30127969146138E-5 1.30127969146138E-5 0.0 1.310076342175659 1.30127969146138E-4 52-53 1.30127969146138E-5 1.30127969146138E-5 0.0 1.5096275828612615 1.366343676034449E-4 54-55 1.30127969146138E-5 1.30127969146138E-5 0.0 1.736330024309206 1.431407660607518E-4 56-57 1.30127969146138E-5 1.30127969146138E-5 0.0 1.9933587889666575 1.431407660607518E-4 58-59 1.30127969146138E-5 1.30127969146138E-5 0.0 2.2784041053812727 1.431407660607518E-4 60-61 1.30127969146138E-5 1.30127969146138E-5 0.0 2.5667806978060295 1.8217915680459321E-4 62-63 1.30127969146138E-5 1.30127969146138E-5 0.0 2.856940043408088 2.082047506338208E-4 64-65 1.30127969146138E-5 1.30127969146138E-5 0.0 3.151907617470096 2.147111490911277E-4 66-67 1.30127969146138E-5 1.30127969146138E-5 0.0 3.48399419473104 2.212175475484346E-4 68-69 1.30127969146138E-5 1.30127969146138E-5 0.0 3.854000062201169 2.212175475484346E-4 70-71 1.30127969146138E-5 1.30127969146138E-5 0.0 4.242542658876165 2.212175475484346E-4 72-73 1.30127969146138E-5 1.30127969146138E-5 0.0 4.623609403723716 2.472431413776622E-4 74-75 1.30127969146138E-5 1.30127969146138E-5 0.0 5.000089137658865 2.472431413776622E-4 76-77 1.30127969146138E-5 1.30127969146138E-5 0.0 5.412861562188873 2.472431413776622E-4 78-79 1.30127969146138E-5 1.30127969146138E-5 0.0 5.861842094133792 2.472431413776622E-4 80-81 1.30127969146138E-5 1.30127969146138E-5 0.0 6.325910964101206 2.537495398349691E-4 82-83 1.30127969146138E-5 1.30127969146138E-5 0.0 6.79582908628174 2.60255938292276E-4 84-85 1.30127969146138E-5 1.30127969146138E-5 0.0 7.26116670394833 2.60255938292276E-4 86-87 1.30127969146138E-5 1.30127969146138E-5 0.0 7.744013039863532 2.732687352068898E-4 88 1.30127969146138E-5 1.30127969146138E-5 0.0 8.126725903520782 2.862815321215036E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13155 0.0 53.006855 1 GTATCAA 23195 0.0 40.218525 1 TACCTGG 6440 0.0 32.782364 2 ATCAACG 29500 0.0 30.90324 3 CAACGCA 29590 0.0 30.896944 5 TATCAAC 30325 0.0 30.508423 2 TCAACGC 30985 0.0 29.58892 4 AACGCAG 31640 0.0 29.191599 6 GTACATG 30935 0.0 28.244484 1 TACATGG 31670 0.0 27.15364 2 ACCTGGG 7610 0.0 26.943253 3 GTACCTG 8065 0.0 26.764402 1 ACATGGG 32110 0.0 25.819595 3 ACGCAGA 35870 0.0 25.48761 7 GTGGTAT 6185 0.0 25.26438 1 CGCAGAG 36780 0.0 24.805988 8 CATGGGG 20790 0.0 23.403473 4 GTACTAG 2845 0.0 22.596563 1 GCAGAGT 40250 0.0 22.503395 9 TGGTATC 6685 0.0 22.457542 2 >>END_MODULE