FastQCFastQC Report
Mon 6 Jun 2016
ERR1211326_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211326_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3826637
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199250.5206921900352711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159470.4167366802756572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90440.2363432956927976No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76430.19973151359797126No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40460.10573252702046208No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39300.10270114463430945No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81750.054.3331831
TACCTGG39200.052.811052
ACCTGGG46350.044.209713
GTATCAA148250.041.7995871
GTACCTG53750.039.392631
TATCAAC186050.033.0777742
ATCAACG185400.032.9037863
CAACGCA186600.032.6665735
CCTGGGG45000.032.2716524
TCAACGC191200.031.8560774
GTGGTAT37000.031.7920991
AACGCAG196400.031.3002056
GTACATG195700.030.991531
TACATGG203750.029.5346012
TGGTATC38850.029.186652
ACATGGG203700.028.6788023
CATGGGG143800.028.1070184
TGGGGGG103750.027.7685536
ACGCAGA221000.027.7526727
ATGGGGG104500.027.0292135
Produced by FastQC (version 0.11.5)