FastQCFastQC Report
Mon 6 Jun 2016
ERR1211326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211326_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3826637
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177790.4646116158914472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150100.3922504277254414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88990.2325540677101068No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63210.16518420743854198No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39420.10301473591563558No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG40850.054.220652
GGTATCA84000.052.414551
ACCTGGG46900.046.730913
GTATCAA148750.039.444041
GTACCTG56750.039.204941
CCTGGGG44300.033.3703964
CAACGCA185100.031.0502435
ATCAACG184650.031.03323
TATCAAC189150.030.589442
TCAACGC189700.030.3056284
TAACGCA4850.029.9967694
AACGCAG196850.029.4824736
GTACATG190550.028.7468471
TACATGG195850.027.745962
TGGGGGG91200.027.3682146
ACATGGG196350.026.5787223
ATGGGGG94850.026.5132375
CTGGGGG28300.026.0284655
ACGCAGA222800.025.8169527
GTGGTAT39850.025.5599371
Produced by FastQC (version 0.11.5)