##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211326_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3826637 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.083622251078424 33.0 31.0 34.0 30.0 34.0 2 32.19590831322647 33.0 31.0 34.0 30.0 34.0 3 32.2566569549189 34.0 31.0 34.0 30.0 34.0 4 35.91822610819892 37.0 35.0 37.0 35.0 37.0 5 35.826038895249276 37.0 35.0 37.0 35.0 37.0 6 35.84968994968689 37.0 35.0 37.0 35.0 37.0 7 35.76994316419352 37.0 35.0 37.0 33.0 37.0 8 35.748438380750514 37.0 35.0 37.0 33.0 37.0 9 37.35119270523961 39.0 37.0 39.0 34.0 39.0 10-11 37.294052845880074 39.0 37.0 39.0 34.0 39.0 12-13 37.21296336705049 39.0 37.0 39.0 33.0 39.0 14-15 38.413050937415804 40.0 38.0 41.0 33.5 41.0 16-17 38.383503713574086 40.0 38.0 41.0 33.5 41.0 18-19 38.40884476369199 40.0 38.0 41.0 33.5 41.0 20-21 38.38541283116219 40.0 38.0 41.0 34.0 41.0 22-23 38.27424197800836 40.0 38.0 41.0 34.0 41.0 24-25 38.17846728080035 40.0 37.0 41.0 33.0 41.0 26-27 38.067053394403494 40.0 37.0 41.0 33.0 41.0 28-29 37.94744275456491 40.0 37.0 41.0 33.0 41.0 30-31 37.924673806269055 40.0 37.0 41.0 33.0 41.0 32-33 37.8183845240612 40.0 37.0 41.0 33.0 41.0 34-35 37.66610381387103 40.0 36.5 41.0 33.0 41.0 36-37 37.51181886340408 40.0 36.0 41.0 32.0 41.0 38-39 37.39004091582243 40.0 36.0 41.0 32.0 41.0 40-41 37.27345290394673 40.0 36.0 41.0 31.5 41.0 42-43 37.15885188482733 39.0 36.0 41.0 31.0 41.0 44-45 36.99816144045019 39.0 35.0 41.0 31.0 41.0 46-47 36.73517895739784 39.0 35.0 41.0 30.5 41.0 48-49 35.17448519417964 39.0 35.0 40.0 23.0 41.0 50-51 35.89728003988881 38.5 34.5 40.0 28.0 41.0 52-53 35.99649731605062 39.0 35.0 40.0 28.5 41.0 54-55 35.83072813020937 38.0 35.0 40.0 28.0 41.0 56-57 35.62910683715231 38.0 34.5 40.0 28.0 41.0 58-59 35.39939024788607 38.0 34.0 40.0 27.5 41.0 60-61 35.13683829953037 37.5 34.0 40.0 27.0 41.0 62-63 34.799506328925375 37.0 34.0 40.0 26.0 41.0 64-65 34.4696214979367 36.0 33.0 39.0 26.0 41.0 66-67 34.130131757990114 36.0 33.0 39.0 26.0 40.0 68-69 33.76940783774369 35.5 33.0 38.5 26.0 40.0 70-71 33.40249885212525 35.0 32.0 38.0 25.0 40.0 72-73 33.01892144460005 35.0 32.0 37.0 25.0 39.5 74-75 32.65425463664309 35.0 32.0 37.0 24.0 39.0 76-77 31.717395195833838 34.0 30.5 36.0 23.5 38.0 78-79 32.158509155689444 34.5 31.0 36.0 24.0 37.5 80-81 32.05279426817856 34.5 31.0 35.5 24.0 37.0 82-83 31.78418491223495 34.0 31.0 35.0 24.0 37.0 84-85 31.499738673932228 34.0 31.0 35.0 23.0 36.0 86-87 31.25141005013018 34.0 31.0 35.0 22.5 36.0 88-89 31.047281986768017 34.0 31.0 35.0 21.0 35.5 90-91 30.832812989578056 34.0 30.0 35.0 20.0 35.0 92-93 30.63785838583592 34.0 30.0 35.0 20.0 35.0 94-95 30.450181059766056 34.0 30.0 35.0 19.5 35.0 96-97 30.26842394509853 34.0 29.0 35.0 19.0 35.0 98-99 30.057925928171393 34.0 29.0 35.0 18.5 35.0 100 27.67084831929446 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.24876734588074 1101 2 -0.27149217459110986 1101 3 -0.26077578722586026 1101 4 -0.19639003852196169 1101 5 -0.17270000278772812 1101 6 -0.22910756944724397 1101 7 -0.2747483158546373 1101 8 -0.21354535100411454 1101 9 -0.28172699543386415 1101 10-11 -0.3557399952379825 1101 12-13 -0.31845664656691497 1101 14-15 -0.41347997486520427 1101 16-17 -0.47810394442823423 1101 18-19 -0.4858267612293474 1101 20-21 -0.49151905786060013 1101 22-23 -0.5170967496470169 1101 24-25 -0.4641176190752674 1101 26-27 -0.5550352226252997 1101 28-29 -0.5800958658487829 1101 30-31 -0.5673586582136565 1101 32-33 -0.4115070347998824 1101 34-35 -0.4649788827323533 1101 36-37 -0.5608597939205282 1101 38-39 -0.5604789271708555 1101 40-41 -0.4587133966734953 1101 42-43 -0.4564604790838658 1101 44-45 -0.4088682660325986 1101 46-47 -0.559155169558359 1101 48-49 0.7775884072280093 1101 50-51 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68-69 -0.9006825535056393 1105 70-71 -1.0053098525982094 1105 72-73 -0.7492117636848619 1105 74-75 -0.889401890590328 1105 76-77 -0.7285120281509627 1105 78-79 -0.590480431501959 1105 80-81 -0.642895525733703 1105 82-83 -0.6385676382355499 1105 84-85 -0.600891763774122 1105 86-87 -0.6985226745631437 1105 88-89 -0.8311466911265661 1105 90-91 -0.7763850361359026 1105 92-93 -0.6408786886358477 1105 94-95 -0.7182748698616805 1105 96-97 -0.6109195859399925 1105 98-99 -0.776831856888041 1105 100 -0.9514175321763823 1106 1 -0.30515238769971376 1106 2 -0.2683739983355409 1106 3 -0.33633938352414106 1106 4 -0.20240276551353276 1106 5 -0.19380445290224202 1106 6 -0.3222451821265153 1106 7 -0.2024548678490703 1106 8 -0.2932115257612935 1106 9 -0.3502624682402029 1106 10-11 -0.41586205777847596 1106 12-13 -0.3334001053038378 1106 14-15 -0.49763748988244316 1106 16-17 -0.5173912155703491 1106 18-19 -0.5522906985315501 1106 20-21 -0.5147795279341025 1106 22-23 -0.46420535666332796 1106 24-25 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1.1274786034822384 2310 98-99 1.0285865858391467 2310 100 1.4468896885255518 2311 1 0.3459478806056353 2311 2 0.39323234536551155 2311 3 0.3854772083435236 2311 4 0.25554417082358327 2311 5 0.2941212786573075 2311 6 0.40215564305492535 2311 7 0.3638745788212816 2311 8 0.20874350406683106 2311 9 0.4685603077385565 2311 10-11 0.38466836511381786 2311 12-13 0.4564911186202991 2311 14-15 0.4012556186176255 2311 16-17 0.4397820472000191 2311 18-19 0.6434595326205752 2311 20-21 0.671617353939773 2311 22-23 0.6302609906474927 2311 24-25 0.562919170843216 2311 26-27 0.730309854797774 2311 28-29 0.7370087668036973 2311 30-31 0.6202973223664827 2311 32-33 0.5935855836116133 2311 34-35 0.7537752840496523 2311 36-37 0.7885771279708536 2311 38-39 0.8040508565356248 2311 40-41 0.7835053782227064 2311 42-43 0.7833215850936384 2311 44-45 0.774357167289331 2311 46-47 0.8714105779655128 2311 48-49 -5.976992261203733 2311 50-51 -1.1068480375733216 2311 52-53 0.3408859815153633 2311 54-55 0.7437831981850493 2311 56-57 0.8165739279423363 2311 58-59 0.9278531417411386 2311 60-61 0.7187178779180243 2311 62-63 0.6172844135695641 2311 64-65 0.6657652450027385 2311 66-67 0.8225937448977589 2311 68-69 0.7287193411999837 2311 70-71 0.7461496028884014 2311 72-73 0.20809993160111873 2311 74-75 0.583622639478584 2311 76-77 0.7168405318886357 2311 78-79 0.623156808659779 2311 80-81 0.6791139029787239 2311 82-83 0.7181747951446198 2311 84-85 0.768067367118725 2311 86-87 0.7501757620857603 2311 88-89 0.7370608931762561 2311 90-91 0.852433101984051 2311 92-93 0.7747435800069091 2311 94-95 0.877630293445474 2311 96-97 0.8493128769698899 2311 98-99 0.9587682804784983 2311 100 1.2148043065941216 2312 1 0.09713091071991897 2312 2 0.2680659974159241 2312 3 0.24520856603186303 2312 4 0.03512794172034006 2312 5 0.22774119361122303 2312 6 0.14663390393366882 2312 7 0.2699605452894147 2312 8 0.360535229998419 2312 9 0.37242031867263137 2312 10-11 0.2930327736261873 2312 12-13 0.3371518283054371 2312 14-15 0.37037556279518924 2312 16-17 0.4836117647139204 2312 18-19 0.3363832221933123 2312 20-21 0.39630055169437384 2312 22-23 0.648159523620528 2312 24-25 0.7680108687327589 2312 26-27 0.5515155865145829 2312 28-29 0.7733383466394272 2312 30-31 0.7655723337447142 2312 32-33 0.7793466844293917 2312 34-35 0.34530426725882535 2312 36-37 0.6813240303172492 2312 38-39 0.8440269564471521 2312 40-41 0.5965882554245283 2312 42-43 0.6306716124077241 2312 44-45 0.7747737888545174 2312 46-47 0.862459016842628 2312 48-49 -4.571911134466589 2312 50-51 -0.7094183362642923 2312 52-53 0.551375613662465 2312 54-55 0.8202347324053036 2312 56-57 0.9668817362681423 2312 58-59 0.9901917127534361 2312 60-61 1.0041368299897684 2312 62-63 0.893915224828497 2312 64-65 0.972011002562958 2312 66-67 0.9000416788071703 2312 68-69 0.9910627441362294 2312 70-71 0.9693940900113276 2312 72-73 0.9990194699392774 2312 74-75 0.7366319469457139 2312 76-77 0.982044845256425 2312 78-79 0.6701356112837047 2312 80-81 0.8451576945577948 2312 82-83 0.8648946889351379 2312 84-85 0.9064776177183376 2312 86-87 1.0350300753585877 2312 88-89 1.0151474534013367 2312 90-91 0.7193054735703974 2312 92-93 0.9099331106414112 2312 94-95 1.0438295927954009 2312 96-97 1.0800145630581497 2312 98-99 1.1131833296639009 2312 100 0.753413383479316 2313 1 -0.010448594904907793 2313 2 0.17528649618224534 2313 3 0.23471957821226397 2313 4 0.04814265851869237 2313 5 0.14421859475942966 2313 6 0.27560249073094667 2313 7 0.13771491286249926 2313 8 0.23617144582071603 2313 9 0.39649995064545607 2313 10-11 0.29168926802147865 2313 12-13 0.129948098992422 2313 14-15 0.2142387687883982 2313 16-17 0.0445773456290226 2313 18-19 0.40666264673584607 2313 20-21 0.3529831147152507 2313 22-23 0.44504797182178635 2313 24-25 0.30375224734085293 2313 26-27 0.42353298098783654 2313 28-29 0.39227650218833787 2313 30-31 0.5340269144832419 2313 32-33 0.4377573834739792 2313 34-35 0.3453600799391552 2313 36-37 -0.4010636679524282 2313 38-39 0.15159388882936753 2313 40-41 0.3161273822166848 2313 42-43 0.4590784763824729 2313 44-45 0.4210416646864985 2313 46-47 0.3044598439783357 2313 48-49 -5.394688381647718 2313 50-51 -1.378401126567944 2313 52-53 0.09409783508100844 2313 54-55 0.29633600430857143 2313 56-57 0.2420542810667925 2313 58-59 0.3055979413459653 2313 60-61 0.6687932398452645 2313 62-63 0.7414679926911418 2313 64-65 0.8286905543666094 2313 66-67 0.887161184323233 2313 68-69 0.9312962124767452 2313 70-71 0.7679032348266617 2313 72-73 0.513137359247338 2313 74-75 0.7330334654199717 2313 76-77 0.7672644163421296 2313 78-79 0.6597400635350823 2313 80-81 -0.18380549564576398 2313 82-83 0.2296152423342761 2313 84-85 0.5748431854394838 2313 86-87 0.6868400785298583 2313 88-89 0.6693048209470227 2313 90-91 0.7620125106567954 2313 92-93 0.9063683578264374 2313 94-95 0.7913588300730581 2313 96-97 0.8073075741922651 2313 98-99 0.9902376902911705 2313 100 1.0378599899811434 2314 1 0.09771966614107441 2314 2 0.20922479678799988 2314 3 0.2699635194047829 2314 4 0.09104856895059754 2314 5 0.19590106530399964 2314 6 0.3424589690123341 2314 7 0.16901822723279025 2314 8 0.29983030753283657 2314 9 0.4804212047448999 2314 10-11 0.314416382898024 2314 12-13 0.3240434348126229 2314 14-15 0.3535177594500212 2314 16-17 0.460369693111609 2314 18-19 0.5470148828177273 2314 20-21 0.602999647863534 2314 22-23 0.35014037777686724 2314 24-25 0.4321133709587741 2314 26-27 0.5209354195592582 2314 28-29 0.61921978125973 2314 30-31 0.5032674073190293 2314 32-33 0.47422256315381617 2314 34-35 0.20722982852559113 2314 36-37 0.3340140207325675 2314 38-39 0.41127609806464704 2314 40-41 0.15182452795610146 2314 42-43 0.18106790080444313 2314 44-45 0.4282276453417495 2314 46-47 0.47916655297388644 2314 48-49 -6.5158138496717015 2314 50-51 -1.6472096588199676 2314 52-53 -0.8529177529852063 2314 54-55 -0.48262594448708995 2314 56-57 -0.20189399277689546 2314 58-59 0.27423309950604136 2314 60-61 0.4562030737920537 2314 62-63 0.3945330622305221 2314 64-65 0.5088320388591185 2314 66-67 0.6153117606884138 2314 68-69 0.6181451222502474 2314 70-71 0.33194646078059264 2314 72-73 0.4944964484575891 2314 74-75 0.5229875168566309 2314 76-77 0.5474765177611403 2314 78-79 0.3899437197112263 2314 80-81 0.4531847234745676 2314 82-83 0.4871134151320007 2314 84-85 0.5699475868605006 2314 86-87 0.5840636873220504 2314 88-89 0.470801299864565 2314 90-91 0.6453611340067162 2314 92-93 0.7770725451268241 2314 94-95 0.7701760421021042 2314 96-97 0.7469185263133724 2314 98-99 0.8939044204733158 2314 100 -0.40860326007645753 2315 1 0.08729575562795588 2315 2 0.2082197556402292 2315 3 0.25510069365881805 2315 4 0.1223206470558651 2315 5 0.2071559418577209 2315 6 0.27555417195588205 2315 7 0.33187969919328 2315 8 0.28594682754766154 2315 9 0.4392371929988883 2315 10-11 0.1809999925681609 2315 12-13 0.39082109168840873 2315 14-15 0.21587200249114602 2315 16-17 0.3239718704961305 2315 18-19 0.43718789743320485 2315 20-21 0.32566502886968607 2315 22-23 0.3488797442682383 2315 24-25 0.41233953294229764 2315 26-27 0.4190560322204604 2315 28-29 0.5467162366330811 2315 30-31 0.5454204668672702 2315 32-33 0.5839432023124616 2315 34-35 0.5431646836397448 2315 36-37 0.4812349482835572 2315 38-39 0.5394338952679334 2315 40-41 0.570223949923772 2315 42-43 0.5978403690934186 2315 44-45 0.6496186787310023 2315 46-47 0.47569599241145255 2315 48-49 -5.014420951757842 2315 50-51 -1.0285214038694477 2315 52-53 0.18350357670106376 2315 54-55 0.1508431486162678 2315 56-57 0.4679896224680036 2315 58-59 0.5480986679746351 2315 60-61 0.49520094775871115 2315 62-63 0.3160067466390828 2315 64-65 0.49049927141841465 2315 66-67 -0.18984167938920393 2315 68-69 0.5330386091776305 2315 70-71 0.5176952941654704 2315 72-73 0.5893696345439778 2315 74-75 0.6923206369034034 2315 76-77 0.6123016717923591 2315 78-79 0.5954086631333553 2315 80-81 0.44084561546194934 2315 82-83 0.4551803368117646 2315 84-85 0.45823446600375917 2315 86-87 0.5281280974881533 2315 88-89 0.5199297905630829 2315 90-91 0.5693680172018247 2315 92-93 0.6155323804644475 2315 94-95 0.27357439318109655 2315 96-97 0.33767668776734183 2315 98-99 0.2603381898954886 2315 100 0.40719260032243554 2316 1 0.19500034122495435 2316 2 0.19852814535908436 2316 3 0.1305203284741836 2316 4 -0.062401083354885145 2316 5 0.14674554246426652 2316 6 0.1218271980373089 2316 7 0.18348608838257263 2316 8 0.2714359965298314 2316 9 0.3957201188996464 2316 10-11 0.2789837250397298 2316 12-13 0.31227640702728365 2316 14-15 0.39959017165515576 2316 16-17 0.3827789644911377 2316 18-19 0.08591248407230978 2316 20-21 0.4056721215886441 2316 22-23 0.5261666144438308 2316 24-25 0.5328203172034804 2316 26-27 0.5517398553980186 2316 28-29 0.6238346680256441 2316 30-31 0.5913040846005586 2316 32-33 0.5551070994159346 2316 34-35 0.5255676691746487 2316 36-37 0.32329514388096925 2316 38-39 0.40141245547788884 2316 40-41 0.030140783274653415 2316 42-43 0.018023089018221583 2316 44-45 0.30896958329192614 2316 46-47 0.29540986978928885 2316 48-49 -5.239062886421884 2316 50-51 -1.12339422939813 2316 52-53 0.23669737381527511 2316 54-55 0.5056981519605799 2316 56-57 0.3300466066694199 2316 58-59 0.2492126076663297 2316 60-61 0.4362117505970744 2316 62-63 0.39300395314992187 2316 64-65 0.42366421913752816 2316 66-67 0.496454645263789 2316 68-69 0.18654652760817214 2316 70-71 -0.20536513431132164 2316 72-73 -0.1386538362945302 2316 74-75 0.18332527216006156 2316 76-77 0.09380052175104936 2316 78-79 0.23525166627776173 2316 80-81 0.15104610440230815 2316 82-83 0.028648729550610597 2316 84-85 0.07630005717177823 2316 86-87 0.11511838142022057 2316 88-89 0.0770205954431269 2316 90-91 0.11942139868482826 2316 92-93 0.21898106884312796 2316 94-95 0.3389823946613184 2316 96-97 0.37207743808555804 2316 98-99 0.39415690127499303 2316 100 -0.3244213601050845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 7.0 11 4.0 12 13.0 13 119.0 14 407.0 15 1231.0 16 3043.0 17 6527.0 18 10929.0 19 15339.0 20 20033.0 21 26898.0 22 33336.0 23 35536.0 24 35549.0 25 37785.0 26 43030.0 27 51803.0 28 63966.0 29 77870.0 30 95571.0 31 117867.0 32 148710.0 33 194928.0 34 275881.0 35 377119.0 36 566666.0 37 845150.0 38 664927.0 39 76392.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.946543757600345 19.219188996738712 13.739437937787741 23.094829307873198 2 16.189063263166943 20.566141331233627 40.34954532399895 22.895250081600473 3 18.65567423258887 27.188843871059458 30.4168260757763 23.738655820575378 4 12.974210239893438 17.09355107008188 37.38007027060088 32.55216841942379 5 12.871477043946925 37.479694853102586 33.63303242342581 16.01579567952468 6 29.407571191100697 36.11920858968332 19.58390618185106 14.889314037364922 7 27.271838763864658 33.03538669473658 22.38940416595673 17.303370375442036 8 26.751675751138183 34.49168729695834 20.645666933142177 18.110970018761304 9 27.900043070464115 16.49737291076887 20.753299281183224 34.849284737583794 10-11 25.319445433502523 26.106503370818817 28.01845244822907 20.55559874744959 12-13 25.533034149360102 24.142803421599833 28.069416674876674 22.254745754163388 14-15 23.51823928626494 25.331296728452973 26.449042630199422 24.70142135508267 16-17 22.275226567115073 27.79799068395901 27.335180827075973 22.591601921849943 18-19 21.832656193937392 26.901401935955775 29.684093369713406 21.581848500393427 20-21 22.850467839237346 26.438528890265207 29.30492456165796 21.40607870883949 22-23 22.775103825090916 26.02864715357751 29.285548529932882 21.910700491398693 24-25 22.145874339759516 25.889057647500312 29.624335839625193 22.340732173114976 26-27 21.95199775468437 26.064921033069933 29.474419225149205 22.508661987096488 28-29 21.909203874462808 26.773619889222665 29.43447111596602 21.882705120348508 30-31 22.955807277340988 26.177821449431406 29.5845458097208 21.281825463506802 32-33 22.771665248611008 26.3581677980403 29.137887933833884 21.73227901951481 34-35 22.690865398312503 26.56188617432277 29.201093179832316 21.54615524753241 36-37 22.515018454997435 26.464539508541108 29.05380662257472 21.966635413886742 38-39 22.55845118888891 26.721594626377392 29.014187624598154 21.705766560135544 40-41 22.584530678688946 26.12778786400244 29.45968731861456 21.827994138694056 42-43 22.331232644621565 26.49660187064045 28.75689919372911 22.41526629100887 44-45 21.725441431439545 26.777734742317776 29.274205224783618 22.22261860145906 46-47 21.823190593993743 26.30375332559631 28.804724591163517 23.068331489246425 48-49 22.246477483563883 26.595243776177828 28.574865476984673 22.583413263273616 50-51 22.114445181515514 26.715846557486056 28.57646746406256 22.593240796935877 52-53 21.975161369029873 27.02904949652183 28.14684147184395 22.84894766260435 54-55 21.898094746882062 26.647434805965414 28.639965689998103 22.81450475715442 56-57 22.114624955277304 26.745361188200683 28.117462641358347 23.022551215163666 58-59 21.778672282497624 26.85104457905573 28.601588050782915 22.768695087663726 60-61 22.451397778957 27.09551200977513 28.2104501423057 22.242640068962174 62-63 22.543827932349725 26.89703034650074 28.206187323681764 22.352954397467773 64-65 22.137179418317153 26.51274537748014 28.15163373272783 23.198441471474872 66-67 22.01414800141574 26.466073707417326 28.111206260629167 23.408572030537766 68-69 21.557113347087267 26.631948551491313 28.46971270313136 23.34122539829006 70-71 21.760150959553258 26.381248414489473 28.46350323035722 23.395097395600054 72-73 21.60027225685406 27.041384035073456 27.790508597027706 23.567835111044776 74-75 21.718539511650366 26.99364176133286 27.75470425637539 23.53311447064138 76-77 21.479854358718626 26.586943459328044 28.05058292841848 23.882619253534855 78-79 21.23674159021207 26.552519440876342 28.189133494235357 24.021605474676225 80-81 21.292362749161512 26.861070618998813 27.754267961554763 24.09229867028491 82-83 21.800806719508806 26.67958316965547 27.96204659925099 23.557563511584732 84-85 21.61201004557521 26.98174795936184 27.491737598032707 23.91450439703024 86-87 21.046795124383376 27.15452892193668 27.856936082843397 23.941739870836543 88-89 21.030376129478505 26.60556778073517 28.053898664675785 24.31015742511054 90-91 21.588597978558152 26.288624090177354 28.066226803067423 24.056551128197075 92-93 21.373026203938334 26.592003500113627 27.852457351839433 24.182512944108606 94-95 20.91660321475459 26.220338055061582 28.006003572298006 24.857055157885824 96-97 20.794022928068003 26.29645609852792 28.079905626280382 24.829615347123696 98-99 20.557742777007178 26.378583333921412 27.872606743681605 25.191067145389805 100 20.37910083021764 26.377199783962514 28.00759791094048 25.23610147487937 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2550.0 1 1872.0 2 3145.5 3 4472.0 4 2769.0 5 1767.5 6 2428.5 7 4169.0 8 6332.5 9 7402.0 10 6303.5 11 4773.5 12 4567.0 13 4842.5 14 5259.5 15 5754.0 16 6117.5 17 6589.5 18 7041.5 19 7333.0 20 7710.5 21 8302.5 22 9122.0 23 10139.5 24 11347.5 25 12960.5 26 15410.5 27 18438.5 28 21407.5 29 26005.0 30 33217.5 31 40530.5 32 49783.5 33 61118.0 34 72750.0 35 83434.5 36 95683.0 37 108858.0 38 114530.5 39 115907.5 40 113859.5 41 110860.5 42 111255.5 43 114943.0 44 121039.5 45 130291.0 46 137672.0 47 141448.5 48 147650.5 49 154526.0 50 159699.0 51 159346.5 52 153361.0 53 145403.0 54 138305.5 55 128815.5 56 116316.0 57 103459.5 58 90840.0 59 77219.5 60 61021.0 61 47315.5 62 37873.5 63 29340.0 64 21561.0 65 15795.0 66 11747.5 67 8954.0 68 6813.5 69 5022.5 70 3813.5 71 2856.0 72 2161.0 73 1679.0 74 1240.0 75 835.5 76 583.5 77 356.5 78 186.0 79 103.5 80 65.0 81 43.0 82 30.0 83 20.0 84 12.5 85 10.0 86 7.0 87 2.5 88 1.0 89 2.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03013089561408621 2 0.0017247520472937464 3 1.3066303388588988E-4 4 0.022343378794487168 5 5.487847423207375E-4 6 0.0 7 2.613260677717798E-5 8 0.003763095375913629 9 0.009120279765235113 10-11 0.00116290100158442 12-13 1.5679564066306786E-4 14-15 1.8292824744024585E-4 16-17 3.0052497793754674E-4 18-19 0.0 20-21 5.226521355435596E-5 22-23 2.351934609946018E-4 24-25 2.613260677717798E-5 26-27 9.146412372012292E-5 28-29 0.001097569484641475 30-31 0.0026655258912721535 32-33 0.010152517732933644 34-35 0.019181333374448634 36-37 0.022055920119938212 38-39 0.021899124479275146 40-41 0.0048606648605551035 42-43 0.005056659411383939 44-45 0.008754423270354623 46-47 0.011589811105678433 48-49 1.2084501351970411 50-51 0.017848570428812556 52-53 0.01833202365419035 54-55 0.009081080855069347 56-57 0.007317129897609833 58-59 0.00812724070770235 60-61 0.005004394197829582 62-63 0.0073563288077756 64-65 0.01882854318295673 66-67 0.028197082712575035 68-69 0.027818159914305956 70-71 0.025518490517914296 72-73 0.021899124479275146 74-75 0.008924285214406278 76-77 0.01369348595124126 78-79 0.007630721178935969 80-81 0.002691658498049332 82-83 0.01369348595124126 84-85 0.022813765716476374 86-87 0.02259163855887036 88-89 0.03133299552583639 90-91 0.019703985509992195 92-93 0.012426054522548129 94-95 0.006493952784128728 96-97 0.00386762580302234 98-99 0.016359011842513413 100 0.03593233431861972 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3826637.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.606585808560574 #Duplication Level Percentage of deduplicated Percentage of total 1 78.00474123513224 45.715915606713416 2 13.567794431703362 15.90324217189067 3 3.764292248466082 6.618369500046805 4 1.5613664440396489 3.6602542592486706 5 0.8224580754579993 2.410072988663641 6 0.5072939220445583 1.7838458863479383 7 0.3387365778972026 1.3896536023321395 8 0.23135353326476749 1.0847072559516215 9 0.18220580165043215 0.9610613954319259 >10 0.9120198408233995 9.588818646023416 >50 0.05991856065981095 2.4421091635843974 >100 0.04387537596385183 4.85591232859196 >500 0.002984613003911962 1.1832888862456032 >1k 7.461532499332909E-4 0.9031702375415529 >5k 1.0659332141904156E-4 0.4754020755795413 >10k+ 1.0659332141904156E-4 1.024175995806674 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17779 0.4646116158914472 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15010 0.3922504277254414 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8899 0.2325540677101068 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6321 0.16518420743854198 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3942 0.10301473591563558 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.6132606777177975E-5 0.0 2 0.0 0.0 0.0 2.6132606777177975E-5 0.0 3 0.0 0.0 0.0 2.6132606777177975E-5 0.0 4 0.0 0.0 0.0 2.6132606777177975E-5 0.0 5 0.0 0.0 0.0 2.6132606777177975E-5 0.0 6 0.0 0.0 0.0 2.6132606777177975E-5 2.6132606777177975E-5 7 0.0 0.0 0.0 2.6132606777177975E-5 2.6132606777177975E-5 8 0.0 0.0 0.0 2.6132606777177975E-5 2.6132606777177975E-5 9 0.0 0.0 0.0 5.226521355435595E-5 2.6132606777177975E-5 10-11 0.0 0.0 0.0 7.839782033153392E-5 2.6132606777177975E-5 12-13 0.0 0.0 0.0 1.045304271087119E-4 2.6132606777177975E-5 14-15 0.0 0.0 0.0 1.045304271087119E-4 5.226521355435595E-5 16-17 0.0 0.0 0.0 1.3066303388588988E-4 5.226521355435595E-5 18-19 0.0 0.0 0.0 2.090608542174238E-4 7.839782033153393E-5 20-21 0.0 0.0 0.0 3.397238881033137E-4 9.146412372012291E-5 22-23 0.0 0.0 0.0 5.357184389321486E-4 1.3066303388588988E-4 24-25 0.0 0.0 0.0 0.0018031498676252802 1.5679564066306786E-4 26-27 0.0 0.0 0.0 0.003906824713188107 1.8292824744024585E-4 28-29 0.0 0.0 0.0 0.008689091753411676 1.959945508288348E-4 30-31 0.0 0.0 0.0 0.023597743919791712 2.090608542174238E-4 32-33 0.0 0.0 0.0 0.05959540975535437 2.090608542174238E-4 34-35 0.0 0.0 0.0 0.11206968416392775 2.090608542174238E-4 36-37 0.0 0.0 0.0 0.19407380423071224 2.090608542174238E-4 38-39 0.0 0.0 0.0 0.3176287690732097 2.090608542174238E-4 40-41 0.0 0.0 0.0 0.46891042970629304 2.6132606777177976E-4 42-43 0.0 0.0 0.0 0.6169647134023948 2.6132606777177976E-4 44-45 0.0 0.0 0.0 0.7903284267621935 2.8745867454895775E-4 46-47 0.0 0.0 0.0 0.9811879203593129 3.397238881033137E-4 48-49 0.0 0.0 0.0 1.187818442146459 3.397238881033137E-4 50-51 0.0 0.0 0.0 1.4190528132143183 3.397238881033137E-4 52-53 0.0 0.0 0.0 1.6516068809244253 3.397238881033137E-4 54-55 0.0 0.0 0.0 1.9198450231887687 3.397238881033137E-4 56-57 0.0 0.0 0.0 2.206911708636069 3.397238881033137E-4 58-59 0.0 0.0 0.0 2.5447540490514253 3.397238881033137E-4 60-61 0.0 0.0 0.0 2.878480503899377 3.397238881033137E-4 62-63 0.0 0.0 0.0 3.2394371350091475 3.397238881033137E-4 64-65 0.0 1.3066303388588987E-5 0.0 3.5850408596373264 3.527901914919027E-4 66-67 0.0 2.6132606777177975E-5 0.0 3.986699025802552 3.789227982690807E-4 68-69 0.0 2.6132606777177975E-5 0.0 4.417181457243005 3.9198910165766967E-4 70-71 0.0 2.6132606777177975E-5 0.0 4.853177868713442 3.9198910165766967E-4 72-73 0.0 2.6132606777177975E-5 0.0 5.269235101212892 5.226521355435595E-4 74-75 0.0 2.6132606777177975E-5 0.0 5.6973917306501765 5.226521355435595E-4 76-77 0.0 2.6132606777177975E-5 0.0 6.175500837942036 5.226521355435595E-4 78-79 0.0 2.6132606777177975E-5 0.0 6.690966506621872 5.226521355435595E-4 80-81 0.0 2.6132606777177975E-5 0.0 7.198866785639714 5.226521355435595E-4 82-83 0.0 2.6132606777177975E-5 0.0 7.703997008339176 5.487847423207375E-4 84-85 0.0 2.6132606777177975E-5 0.0 8.228400028536807 5.487847423207375E-4 86-87 0.0 2.6132606777177975E-5 0.0 8.784920022463588 5.487847423207375E-4 88 0.0 2.6132606777177975E-5 0.0 9.219505273168059 5.749173490979155E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4085 0.0 54.22065 2 GGTATCA 8400 0.0 52.41455 1 ACCTGGG 4690 0.0 46.73091 3 GTATCAA 14875 0.0 39.44404 1 GTACCTG 5675 0.0 39.20494 1 CCTGGGG 4430 0.0 33.370396 4 CAACGCA 18510 0.0 31.050243 5 ATCAACG 18465 0.0 31.0332 3 TATCAAC 18915 0.0 30.58944 2 TCAACGC 18970 0.0 30.305628 4 TAACGCA 485 0.0 29.996769 4 AACGCAG 19685 0.0 29.482473 6 GTACATG 19055 0.0 28.746847 1 TACATGG 19585 0.0 27.74596 2 TGGGGGG 9120 0.0 27.368214 6 ACATGGG 19635 0.0 26.578722 3 ATGGGGG 9485 0.0 26.513237 5 CTGGGGG 2830 0.0 26.028465 5 ACGCAGA 22280 0.0 25.816952 7 GTGGTAT 3985 0.0 25.559937 1 >>END_MODULE