FastQCFastQC Report
Mon 6 Jun 2016
ERR1211325_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211325_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13243714
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422320.3188833585503281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330830.24980152848362627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176450.13323301907606883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA237150.051.170781
GTATCAA449600.043.332051
CAACGCA541950.034.9913835
ATCAACG547450.034.7607843
TATCAAC569750.033.920522
AACGCAG592150.032.493366
TCAACGC607750.031.4123784
GTACTAG74450.031.3990971
GTGGTAT99800.029.6445981
ACGCAGA653750.028.9155927
CGCAGAG667750.028.1881248
GTACATG679900.026.4544241
GCATCGT81150.025.9972218
TAGGCAT101750.025.6749445
GCAGAGT715850.025.6661039
TACATGG698450.025.4613882
ACATGGG700800.024.7473643
TGGTATC122250.023.4688422
CTAGGCA111100.023.2609584
GAGTACT333150.023.02683312-13
Produced by FastQC (version 0.11.5)