FastQCFastQC Report
Mon 6 Jun 2016
ERR1211325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211325_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13243714
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379250.286362269677524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316240.23878498131264386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170660.12886113366688529No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA251350.051.3278921
GTATCAA453200.041.5873641
CAACGCA552950.033.114095
ATCAACG557350.032.858133
TATCAAC586300.031.8813952
AACGCAG603600.030.9493926
TCAACGC616400.029.9007974
GTGGTAT101800.028.720891
GTACTAG71650.027.6410061
ACGCAGA673050.027.0309077
CGCAGAG688800.026.4060138
CGTTAAC25550.025.3481221
GCAGAGT741050.024.2014949
TGGTATC122250.024.0249962
GTACATG676150.023.8766251
TACATGG690900.023.0209032
GAGTACT346100.022.44346812-13
ACATGGG686500.022.4321213
TAGTACT96700.021.786824
CTGTGCG97100.021.4027639
Produced by FastQC (version 0.11.5)