##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211325_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13243714 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12779368385635 33.0 31.0 34.0 30.0 34.0 2 32.263503953649256 34.0 31.0 34.0 30.0 34.0 3 32.325291681774466 34.0 31.0 34.0 30.0 34.0 4 35.962943249907084 37.0 35.0 37.0 35.0 37.0 5 35.86872156858718 37.0 35.0 37.0 35.0 37.0 6 35.88447402292137 37.0 35.0 37.0 35.0 37.0 7 35.800035171402826 37.0 35.0 37.0 35.0 37.0 8 35.78353609871068 37.0 35.0 37.0 33.0 37.0 9 37.38985189501978 39.0 37.0 39.0 34.0 39.0 10-11 37.346678960297695 39.0 37.0 39.0 34.0 39.0 12-13 37.272074245940374 39.0 37.0 39.0 34.0 39.0 14-15 38.49585146583504 40.0 38.0 41.0 34.0 41.0 16-17 38.48603590352374 40.0 38.0 41.0 34.0 41.0 18-19 38.49781628476725 40.0 38.0 41.0 34.0 41.0 20-21 38.468431098708415 40.0 38.0 41.0 34.0 41.0 22-23 38.36155945379068 40.0 38.0 41.0 34.0 41.0 24-25 38.265265430830055 40.0 38.0 41.0 34.0 41.0 26-27 38.159860746011276 40.0 38.0 41.0 33.0 41.0 28-29 38.06962204861868 40.0 37.0 41.0 33.0 41.0 30-31 38.084565213353294 40.0 37.0 41.0 33.0 41.0 32-33 38.026797505593976 40.0 37.0 41.0 33.0 41.0 34-35 37.90693267764617 40.0 37.0 41.0 33.0 41.0 36-37 37.77185602920751 40.0 37.0 41.0 33.0 41.0 38-39 37.658339911296785 40.0 37.0 41.0 33.0 41.0 40-41 37.548539895983865 40.0 36.0 41.0 32.0 41.0 42-43 37.44058339677224 40.0 36.0 41.0 32.0 41.0 44-45 37.26465929421309 39.5 36.0 41.0 31.5 41.0 46-47 37.010664455605124 39.0 36.0 41.0 31.0 41.0 48-49 35.44644987803271 39.0 35.0 40.5 24.5 41.0 50-51 36.15796894285093 39.0 35.0 40.0 29.0 41.0 52-53 36.24328145412986 39.0 35.0 40.0 29.5 41.0 54-55 36.071215370552395 38.0 35.0 40.0 29.0 41.0 56-57 35.838093566502565 38.0 35.0 40.0 29.0 41.0 58-59 35.58103988050482 38.0 34.0 40.0 28.0 41.0 60-61 35.32347138423557 37.0 34.0 40.0 28.0 41.0 62-63 34.997266552267746 37.0 34.0 40.0 27.5 41.0 64-65 34.66358915633485 36.0 34.0 39.0 27.0 41.0 66-67 34.330224399288596 36.0 33.0 39.0 26.0 40.0 68-69 33.98414689414163 35.0 33.0 38.5 26.0 40.0 70-71 33.65264604022708 35.0 33.0 38.0 26.0 40.0 72-73 33.29594002105452 35.0 32.0 37.0 26.0 39.5 74-75 32.94620832947616 35.0 32.0 37.0 25.5 39.0 76-77 32.020819499726436 34.0 30.5 35.5 24.5 38.0 78-79 32.47063837228741 34.5 32.0 36.0 25.0 38.0 80-81 32.36462407750575 35.0 32.0 35.5 25.0 37.0 82-83 32.114898056542145 34.0 31.5 35.0 25.0 37.0 84-85 31.855321437777953 34.0 31.0 35.0 24.5 36.0 86-87 31.621356592267094 34.0 31.0 35.0 24.0 36.0 88-89 31.41326118942164 34.0 31.0 35.0 24.0 36.0 90-91 31.206752124064295 34.0 31.0 35.0 23.5 35.0 92-93 31.025763845398657 34.0 30.5 35.0 23.0 35.0 94-95 30.8492028746619 34.0 30.0 35.0 22.5 35.0 96-97 30.674880664139984 34.0 30.0 35.0 20.0 35.0 98-99 30.47322393854171 34.0 29.5 35.0 20.0 35.0 100 27.981358929979912 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2772030075887777 1101 2 -0.26587388889116426 1101 3 -0.26781398630422615 1101 4 -0.1640530696320397 1101 5 -0.121392527539399 1101 6 -0.20264963987207096 1101 7 -0.1898201554256289 1101 8 -0.20632453355307234 1101 9 -0.2918156774758316 1101 10-11 -0.2651398830474605 1101 12-13 -0.35182807521207593 1101 14-15 -0.39379123970333296 1101 16-17 -0.3928693879353986 1101 18-19 -0.4435535847752021 1101 20-21 -0.41897872029677075 1101 22-23 -0.5258420035222784 1101 24-25 -0.479245487176847 1101 26-27 -0.5869666088086873 1101 28-29 -0.6014266066349165 1101 30-31 -0.5292104997700946 1101 32-33 -0.4663533944037681 1101 34-35 -0.5648760208436983 1101 36-37 -0.5196547506248663 1101 38-39 -0.5315254720102232 1101 40-41 -0.5190461754106863 1101 42-43 -0.5471911607503799 1101 44-45 -0.46599806104925534 1101 46-47 -0.5010262101288703 1101 48-49 0.6389324675552146 1101 50-51 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1105 68-69 -0.8239163975333454 1105 70-71 -0.8031276678121273 1105 72-73 -0.7858309328344362 1105 74-75 -0.8534974317611415 1105 76-77 -0.8181910996697894 1105 78-79 -0.670392675377208 1105 80-81 -0.6448754738331139 1105 82-83 -0.6226336604283844 1105 84-85 -0.6013575101026056 1105 86-87 -0.6067899981603517 1105 88-89 -0.7472822928103113 1105 90-91 -0.84120707277566 1105 92-93 -0.8028295566964196 1105 94-95 -0.7312508033793499 1105 96-97 -0.7820392445453059 1105 98-99 -0.9085824156950295 1105 100 -0.8384475533973514 1106 1 -0.2846573755681199 1106 2 -0.3034573262049385 1106 3 -0.33165873323508066 1106 4 -0.1942424793326012 1106 5 -0.2247163881901244 1106 6 -0.3182560386840052 1106 7 -0.2981960406458555 1106 8 -0.3790403084052656 1106 9 -0.42987978852109876 1106 10-11 -0.4212353391317336 1106 12-13 -0.44202813658677087 1106 14-15 -0.6023046456728238 1106 16-17 -0.5338041145188512 1106 18-19 -0.5696059301044585 1106 20-21 -0.6146721182496009 1106 22-23 -0.5988295001470689 1106 24-25 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1.1134241641605627 2310 100 1.3475311554589275 2311 1 0.34028287871174 2311 2 0.35883733109369587 2311 3 0.37936205721491945 2311 4 0.2428049255981648 2311 5 0.27837331132088394 2311 6 0.3430516156383092 2311 7 0.38420271327519373 2311 8 0.15536796664916608 2311 9 0.42640107811683947 2311 10-11 0.3755342069124836 2311 12-13 0.4847247901797047 2311 14-15 0.43792006273711337 2311 16-17 0.48738892239317266 2311 18-19 0.6194822503143911 2311 20-21 0.6676026269114459 2311 22-23 0.6557433932245758 2311 24-25 0.6295807917824945 2311 26-27 0.6526970075517511 2311 28-29 0.7254720804425929 2311 30-31 0.6282220265780651 2311 32-33 0.6657042571882457 2311 34-35 0.781118165072634 2311 36-37 0.7875488803169759 2311 38-39 0.8234460111986053 2311 40-41 0.761729439674113 2311 42-43 0.8239592520569019 2311 44-45 0.8011829169752716 2311 46-47 0.8543092390103553 2311 48-49 -6.089673440573112 2311 50-51 -1.0482397579882061 2311 52-53 0.4206788541429347 2311 54-55 0.8404259040350865 2311 56-57 0.9778391983828669 2311 58-59 0.9687091674074111 2311 60-61 0.7931062018712964 2311 62-63 0.6853962678976586 2311 64-65 0.7504823435157277 2311 66-67 0.8856355896686665 2311 68-69 0.8801668140831893 2311 70-71 0.8743967291814485 2311 72-73 0.37363644625907 2311 74-75 0.76657491623466 2311 76-77 0.8372323806964417 2311 78-79 0.7266960125357329 2311 80-81 0.7780192895065099 2311 82-83 0.8212617983483668 2311 84-85 0.8567334628840548 2311 86-87 0.9007767798630333 2311 88-89 0.9171079477562678 2311 90-91 0.9793395375270393 2311 92-93 0.9834323432552416 2311 94-95 1.0317224280624622 2311 96-97 1.0364255932092625 2311 98-99 1.0265157518744097 2311 100 1.237148117170662 2312 1 0.06556473788342032 2312 2 0.19549466053905462 2312 3 0.15834556650261788 2312 4 -0.012635017422120143 2312 5 0.16543209172213835 2312 6 0.09629426008784492 2312 7 0.20479673487690064 2312 8 0.3096789953007075 2312 9 0.235648702701873 2312 10-11 0.19795045339262174 2312 12-13 0.26347383845384087 2312 14-15 0.2708376237149608 2312 16-17 0.3986361612831004 2312 18-19 0.2681677032818328 2312 20-21 0.3607138276170545 2312 22-23 0.5820672492886274 2312 24-25 0.6925513988055059 2312 26-27 0.6292168135688172 2312 28-29 0.7266978670059814 2312 30-31 0.717594619591118 2312 32-33 0.7377724127422454 2312 34-35 0.3070890021683681 2312 36-37 0.6330886450509965 2312 38-39 0.7515308158271168 2312 40-41 0.49309168186243113 2312 42-43 0.5381330292540198 2312 44-45 0.6456680158396821 2312 46-47 0.737582144159532 2312 48-49 -4.706435294742953 2312 50-51 -0.8011096758670817 2312 52-53 0.5005931268490329 2312 54-55 0.7195613862529626 2312 56-57 0.812628415351675 2312 58-59 0.8026848647071105 2312 60-61 0.8826229099800429 2312 62-63 0.6904872797770594 2312 64-65 0.8378392248051441 2312 66-67 0.8146313149482509 2312 68-69 0.7241627527042525 2312 70-71 0.7400798754671456 2312 72-73 0.8891459840846991 2312 74-75 0.5738075003545902 2312 76-77 0.7574300529070399 2312 78-79 0.4110144206473265 2312 80-81 0.6153952171857853 2312 82-83 0.6015003327595778 2312 84-85 0.6664714897690267 2312 86-87 0.8054359131398705 2312 88-89 0.7937690203583507 2312 90-91 0.6109870518028302 2312 92-93 0.776109535596543 2312 94-95 0.9316386175541247 2312 96-97 0.9655649130166957 2312 98-99 1.0096719758528678 2312 100 0.5043998174548072 2313 1 0.10911449058492195 2313 2 0.2513172809164388 2313 3 0.32001994297996106 2313 4 0.10998611923425017 2313 5 0.15495685999044895 2313 6 0.3061840936765776 2313 7 0.204677630758205 2313 8 0.29373272427431374 2313 9 0.4879975975738091 2313 10-11 0.27543112906426614 2313 12-13 0.05686079394963173 2313 14-15 0.1664783429918728 2313 16-17 0.12070839730127858 2313 18-19 0.4269107853409366 2313 20-21 0.38599221599977085 2313 22-23 0.5102987690109941 2313 24-25 0.35901850892391707 2313 26-27 0.4562491518065954 2313 28-29 0.42123422973134694 2313 30-31 0.5716565948804444 2313 32-33 0.4401137166510338 2313 34-35 0.39027645861523297 2313 36-37 -0.3322979713269518 2313 38-39 0.19152153172405662 2313 40-41 0.38361091528817326 2313 42-43 0.5172487920893118 2313 44-45 0.5165374227221662 2313 46-47 0.3868575596978445 2313 48-49 -5.242412375037745 2313 50-51 -1.2414286278795998 2313 52-53 0.16029244458256642 2313 54-55 0.32917417680369 2313 56-57 0.27783905224520566 2313 58-59 0.37494697627338525 2313 60-61 0.6988119448713874 2313 62-63 0.7818143953202323 2313 64-65 0.8064426583347597 2313 66-67 0.8736906352374163 2313 68-69 0.9169770304749321 2313 70-71 0.8851586902733999 2313 72-73 0.6042687274570326 2313 74-75 0.7807806738663317 2313 76-77 0.84504252127482 2313 78-79 0.7759748864074112 2313 80-81 -0.1398860305355285 2313 82-83 0.24804122585012323 2313 84-85 0.6119284164153065 2313 86-87 0.7197402373191686 2313 88-89 0.747244262656853 2313 90-91 0.8265447054041068 2313 92-93 0.8153325442729518 2313 94-95 0.7689479285011096 2313 96-97 0.7907479533739625 2313 98-99 0.9947095209420311 2313 100 1.016193976846349 2314 1 0.045987271559269516 2314 2 0.20183264098088927 2314 3 0.22870410394691731 2314 4 0.061592801979209355 2314 5 0.18614470476846634 2314 6 0.2735380424910616 2314 7 0.10303706077237251 2314 8 0.23073010613255462 2314 9 0.4258625299747081 2314 10-11 0.32634267243644643 2314 12-13 0.2046290775781756 2314 14-15 0.23903021315484807 2314 16-17 0.46656810674017635 2314 18-19 0.5344243305129268 2314 20-21 0.603690893352649 2314 22-23 0.30343943054349864 2314 24-25 0.4323976294748846 2314 26-27 0.4957004172245689 2314 28-29 0.6298381182634074 2314 30-31 0.5358659483578734 2314 32-33 0.5158139686272563 2314 34-35 0.34535511198733104 2314 36-37 0.47449870698335417 2314 38-39 0.5138570564059037 2314 40-41 0.3260749318024381 2314 42-43 0.31168380457452116 2314 44-45 0.5440159364490214 2314 46-47 0.6287663462743893 2314 48-49 -6.293278609779925 2314 50-51 -1.5576061245623904 2314 52-53 -0.874687061287112 2314 54-55 -0.37219619540383064 2314 56-57 -0.11977060384740668 2314 58-59 0.34946853951526435 2314 60-61 0.5633256432579685 2314 62-63 0.4496167825351307 2314 64-65 0.5691298191202776 2314 66-67 0.6832281285289028 2314 68-69 0.726908618488288 2314 70-71 0.4933881505246376 2314 72-73 0.6571692981719366 2314 74-75 0.7035533145241999 2314 76-77 0.6632311058270233 2314 78-79 0.4100279035112351 2314 80-81 0.4814503515078101 2314 82-83 0.49942664155546623 2314 84-85 0.5532104972918894 2314 86-87 0.6236762044610877 2314 88-89 0.540497700940751 2314 90-91 0.7170236135785117 2314 92-93 0.7602189632395913 2314 94-95 0.856644533102493 2314 96-97 0.8828406568446461 2314 98-99 0.9105171451633254 2314 100 -0.4861913912870861 2315 1 0.13621038580775036 2315 2 0.20731814308467733 2315 3 0.29749221662917336 2315 4 0.11320028375519797 2315 5 0.21140323471519196 2315 6 0.2365269122929874 2315 7 0.2990708156149111 2315 8 0.2507109595395889 2315 9 0.40771056154299856 2315 10-11 0.21432710561304447 2315 12-13 0.3800953099762907 2315 14-15 0.24847559857771984 2315 16-17 0.3487684068376282 2315 18-19 0.43853338336804626 2315 20-21 0.33902205318548084 2315 22-23 0.4110510491153363 2315 24-25 0.4549705187459878 2315 26-27 0.5085045131208901 2315 28-29 0.5771522235318756 2315 30-31 0.5825911694302377 2315 32-33 0.679526969984515 2315 34-35 0.6611473029890647 2315 36-37 0.5405522406004479 2315 38-39 0.6247468619087968 2315 40-41 0.6500029217953482 2315 42-43 0.6988802207052558 2315 44-45 0.7490417544022492 2315 46-47 0.6137714401388763 2315 48-49 -4.8307641154335315 2315 50-51 -0.8533205098109065 2315 52-53 0.28952693701773313 2315 54-55 0.2831985521868887 2315 56-57 0.6191921655729047 2315 58-59 0.7335992555959407 2315 60-61 0.6159094001186034 2315 62-63 0.3743701205665815 2315 64-65 0.5605597520101071 2315 66-67 -0.15306474080646382 2315 68-69 0.5862968991902378 2315 70-71 0.5157566378960325 2315 72-73 0.6547729767135664 2315 74-75 0.8051220849660723 2315 76-77 0.7487819883489522 2315 78-79 0.7448608584013172 2315 80-81 0.5585159406781273 2315 82-83 0.5777467949119668 2315 84-85 0.5982886095218056 2315 86-87 0.6089013438048845 2315 88-89 0.6434175920402048 2315 90-91 0.7363941737938049 2315 92-93 0.7772515413247376 2315 94-95 0.3749074356559312 2315 96-97 0.46315866193222277 2315 98-99 0.46371130397250937 2315 100 0.5056241018966432 2316 1 0.22641922972682238 2316 2 0.23907900980786678 2316 3 0.17765536601547183 2316 4 -0.030164396445492514 2316 5 0.16265993301464476 2316 6 0.17597272627750726 2316 7 0.2387695055850827 2316 8 0.3118613046371692 2316 9 0.4600559811349072 2316 10-11 0.3948785152452956 2316 12-13 0.32257553228877356 2316 14-15 0.4373656992772368 2316 16-17 0.4104593962902854 2316 18-19 0.13009839863653383 2316 20-21 0.46505377032351447 2316 22-23 0.5606888675948554 2316 24-25 0.6158544293787855 2316 26-27 0.5927839611381316 2316 28-29 0.6808089958078369 2316 30-31 0.589041053093176 2316 32-33 0.596827246503743 2316 34-35 0.5828164371566231 2316 36-37 0.4576636234417606 2316 38-39 0.5491386176031128 2316 40-41 0.2149763569440637 2316 42-43 0.13237698028697054 2316 44-45 0.4122562938528631 2316 46-47 0.4235276391486238 2316 48-49 -5.147937999241115 2316 50-51 -1.0776777418396293 2316 52-53 0.23097862319509233 2316 54-55 0.48046994707559065 2316 56-57 0.4429441046585936 2316 58-59 0.28015361329178035 2316 60-61 0.40065999881666414 2316 62-63 0.3584383448152906 2316 64-65 0.32927569611528185 2316 66-67 0.41336162510518903 2316 68-69 0.07317327464651413 2316 70-71 -0.25067451488325787 2316 72-73 -0.07932991027864489 2316 74-75 0.10849121824741559 2316 76-77 0.12391313073297283 2316 78-79 0.20595750001908897 2316 80-81 0.15233245913625382 2316 82-83 0.13941303496523716 2316 84-85 0.2935874850413178 2316 86-87 0.3317870474430542 2316 88-89 0.1312970297333358 2316 90-91 0.15613715005203943 2316 92-93 0.2458318165450457 2316 94-95 0.4574344890588087 2316 96-97 0.5598445129412255 2316 98-99 0.5071617596661326 2316 100 -0.22869224704815494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 7.0 10 21.0 11 21.0 12 58.0 13 228.0 14 868.0 15 2474.0 16 6094.0 17 12683.0 18 22637.0 19 35389.0 20 53412.0 21 79217.0 22 99482.0 23 102241.0 24 104109.0 25 114889.0 26 137413.0 27 170640.0 28 212019.0 29 260187.0 30 320944.0 31 401069.0 32 507791.0 33 665835.0 34 943060.0 35 1323886.0 36 2073560.0 37 2994416.0 38 2304486.0 39 294568.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.51857693675656 20.75034194307049 14.605374395656362 23.125706724516583 2 15.228160044459733 20.418652397469085 40.47723232134841 23.87595523672277 3 18.625550261633833 26.19459818630745 29.86021278041635 25.319638771642367 4 12.863876741380922 17.084737053057488 38.58326209472254 31.468124110839046 5 13.423718196393247 38.258305122985995 34.26855545931666 14.049421221304106 6 30.653768665658994 37.54007375276981 17.552987056644113 14.253170524927086 7 27.624249945710066 32.678030956285056 21.95559581953936 17.74212327846552 8 26.61018186755189 35.16372051517121 19.523802727873584 18.702294889403316 9 26.578792641737888 16.02213682962697 20.79015940202831 36.60891112660683 10-11 25.593605944025583 26.394248520978138 26.971767388653344 21.040378146342935 12-13 25.966865158483245 23.83756265147293 27.737393386124708 22.458178803919115 14-15 23.43325216643582 25.23846905466256 25.3476417220128 25.980637056888824 16-17 22.25782202665884 28.236680084518838 26.400988638009792 23.10450925081253 18-19 21.96958450923729 27.494976483934412 28.791958025061255 21.743480981767046 20-21 23.236524313741835 26.49621661734512 28.52234918569609 21.744909883216962 22-23 23.39336662443394 26.155756034416267 28.264388306890094 22.1864890342597 24-25 22.87189250111534 26.429068079453117 28.59202325451189 22.107016164919653 26-27 22.641195792535953 26.725684404556972 28.181935081293734 22.451184721613338 28-29 22.19665555122432 26.795620899548556 28.582217620414408 22.42550592881272 30-31 22.848683319316265 26.50947892599607 28.48253670626935 22.15930104841831 32-33 22.940827281710778 26.82226798748933 27.934086084044544 22.30281864675535 34-35 22.883196671015444 26.73004299057304 28.385888065915104 22.000872272496416 36-37 22.82132043794573 26.494636697930886 28.20908079580633 22.474962068317055 38-39 22.875440635818357 26.521677841656622 28.478399003274045 22.124482519250975 40-41 22.67183364289758 26.076457681381083 28.808409525655954 22.443299150065382 42-43 22.589889964974354 26.16018464985737 28.386381631276365 22.863543753891914 44-45 22.631673956520945 26.63393063155827 28.2053985082792 22.528996903641584 46-47 22.63535505148112 26.540283984396474 27.769926996671835 23.054433967450567 48-49 23.247784014256286 26.528235822572878 27.684226354300552 22.539753808870287 50-51 22.959385211305765 26.55048344016136 27.50756109814767 22.982570250385205 52-53 22.651536211749338 26.832009174970832 27.4629281349082 23.053526478371626 54-55 22.622481068355622 26.781456399534086 27.363335372105958 23.232727160004334 56-57 23.014618298146953 26.570164825680042 27.15218280718505 23.263034068987952 58-59 22.443303583969236 26.768507418009523 27.710355400541104 23.077833597480137 60-61 22.689887916617348 27.005387923507758 27.298768391024947 23.005955768849944 62-63 22.892483658859828 26.663145991877123 27.168668778291238 23.27570157097181 64-65 22.227114793466406 26.626033528927934 27.439576115783787 23.707275561821874 66-67 22.613013499848318 26.51340103707059 27.2899143153725 23.583671147708596 68-69 22.285401334899664 26.61563595991094 27.419979113845628 23.67898359134377 70-71 22.35652068207653 26.273918128928813 27.519095285589323 23.850465903405333 72-73 22.61916764981304 26.60841198693146 27.25284017336607 23.519580189889428 74-75 22.613500750408356 26.808273827442818 27.198533746363445 23.37969167578538 76-77 22.41528693416569 26.696315415300226 27.07170571291646 23.81669193761763 78-79 22.333169292044666 26.626117482509663 27.190066362340755 23.850646863104913 80-81 22.19049602073796 27.018674847881176 27.199644953815493 23.591184177565378 82-83 22.666072740835805 26.531564776568317 27.642932817533644 23.15942966506223 84-85 22.680448771337055 26.61806043350989 27.05101822986754 23.650472565285515 86-87 22.00354861411008 27.00162952577447 27.400801410080554 23.5940204500349 88-89 22.319619438085045 26.344565489143367 27.41289426913818 23.922920803633403 90-91 22.32762641967802 26.319507991341883 27.489783332986555 23.86308225599354 92-93 22.38311318962244 26.53362161904178 27.363264913431216 23.720000277904564 94-95 21.817644444540093 26.298413873529352 27.634862395089215 24.249079286841337 96-97 22.011306149894644 26.271318903576052 27.53143019813512 24.18594474839418 98-99 21.712348735717782 26.494033028353233 27.45184553006421 24.341772705864777 100 21.880650448213814 26.260598048461993 27.20645541496582 24.652296088358376 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1320.0 1 946.0 2 713.0 3 888.5 4 774.5 5 795.5 6 1415.0 7 2863.0 8 4835.5 9 6186.5 10 5952.5 11 5301.5 12 5746.0 13 6747.0 14 7875.5 15 9169.0 16 10557.0 17 11949.5 18 13257.5 19 14632.5 20 16096.0 21 17731.0 22 19597.0 23 21945.0 24 25008.0 25 29182.0 26 35256.0 27 42438.0 28 51117.5 29 65543.0 30 87291.0 31 112877.0 32 149056.0 33 194629.5 34 238360.5 35 280538.5 36 336603.5 37 406207.5 38 440369.0 39 438848.5 40 424572.5 41 408249.5 42 409481.5 43 429305.0 44 457894.0 45 505568.0 46 546493.5 47 566160.5 48 582780.5 49 582351.5 50 564383.5 51 551573.5 52 540567.5 53 519226.0 54 498089.5 55 455461.0 56 406636.5 57 347043.5 58 285789.0 59 249549.0 60 203876.5 61 154901.0 62 119876.0 63 93128.5 64 66994.0 65 43578.0 66 29987.0 67 21915.0 68 16178.5 69 11664.0 70 8617.5 71 6557.5 72 4905.0 73 3466.0 74 2345.5 75 1570.5 76 1137.5 77 746.0 78 456.5 79 277.0 80 150.5 81 72.5 82 54.5 83 43.0 84 27.0 85 14.5 86 6.5 87 5.5 88 8.0 89 5.5 90 3.0 91 3.0 92 2.0 93 1.5 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02972731063204778 2 0.0020311522885498737 3 1.0571052802861794E-4 4 0.022659806758134463 5 3.8508835210425115E-4 6 6.040601601635311E-5 7 4.530451201226484E-5 8 0.0038131297610322907 9 0.0101784136987555 10-11 0.001238323328335239 12-13 8.305827202248553E-5 14-15 1.0948590402964003E-4 16-17 2.9825470408074354E-4 18-19 1.5101504004088278E-5 20-21 7.550752002044139E-5 22-23 2.605009440705228E-4 24-25 3.397838400919863E-5 26-27 9.060902402452968E-5 28-29 9.551701282585837E-4 30-31 0.002537052672686831 32-33 0.009513947522575616 34-35 0.018416284132985655 36-37 0.021172308613731768 38-39 0.020858952405646936 40-41 0.004806053649301095 42-43 0.0053119540334380525 44-45 0.00923079432249896 46-47 0.011915086659225653 48-49 1.2002788643729394 50-51 0.018246392212939663 52-53 0.01867300970105516 54-55 0.009585679666595036 56-57 0.007520548994035963 58-59 0.008139710658203582 60-61 0.00507032996937264 62-63 0.007456367602018587 64-65 0.019261968357214602 66-67 0.028877851031817812 68-69 0.029013764567854605 70-71 0.026197334071092142 72-73 0.023112851878257113 74-75 0.009495070642570507 76-77 0.014316225795875688 78-79 0.008403986978275128 80-81 0.0027673506087491774 82-83 0.014202964515845027 84-85 0.02387925320646459 86-87 0.023044895110238713 88-89 0.03280046669687974 90-91 0.02059467608557539 92-93 0.013274222019593598 94-95 0.006452117585746717 96-97 0.0036168102089791433 98-99 0.015331801940150625 100 0.03563954944964834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.3243714E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.894603092975835 #Duplication Level Percentage of deduplicated Percentage of total 1 67.50287978506849 30.305149955835116 2 15.1326689944501 13.587503364864379 3 6.604351438194314 8.894992095127733 4 3.415375153598067 6.133276477375864 5 2.078991822907192 4.666775636148034 6 1.2724712436735741 3.427625485917027 7 0.8574730510728789 2.6947138603587923 8 0.6103907588248257 2.192260067924871 9 0.44632027830182797 1.803363457203637 >10 1.9327778300498044 14.480332781282243 >50 0.0869461960545235 2.685188346665112 >100 0.05164771404582046 4.5753587997966 >500 0.005327984578970791 1.5960912704812795 >1k 0.002257356818159098 1.6683242443558473 >5k 3.0098090292183166E-5 0.11866605641496752 >10k+ 9.02942708765495E-5 1.1703781002485099 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37925 0.286362269677524 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31624 0.23878498131264386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17066 0.12886113366688529 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.285526401430898E-5 2 0.0 0.0 0.0 0.0 5.285526401430898E-5 3 0.0 0.0 0.0 0.0 5.285526401430898E-5 4 0.0 7.55075200204414E-6 0.0 0.0 6.040601601635312E-5 5 0.0 7.55075200204414E-6 0.0 0.0 6.040601601635312E-5 6 1.510150400408828E-4 7.55075200204414E-6 0.0 0.0 1.3591353603679453E-4 7 1.510150400408828E-4 7.55075200204414E-6 0.0 0.0 1.3591353603679453E-4 8 1.6611654404497108E-4 7.55075200204414E-6 0.0 0.0 1.3591353603679453E-4 9 1.6611654404497108E-4 7.55075200204414E-6 0.0 0.0 2.3407331206336832E-4 10-11 1.6611654404497108E-4 1.510150400408828E-5 0.0 7.55075200204414E-6 2.7182707207358905E-4 12-13 1.6611654404497108E-4 4.152913601124277E-5 0.0 7.55075200204414E-6 3.1713158408585386E-4 14-15 1.6611654404497108E-4 8.305827202248554E-5 0.0 7.55075200204414E-6 4.3794361611856013E-4 16-17 2.793778240756332E-4 9.815977602657382E-5 0.0 1.132612800306621E-5 4.6437124812571457E-4 18-19 2.793778240756332E-4 9.815977602657382E-5 0.0 3.397838400919863E-5 4.907988801328691E-4 20-21 2.793778240756332E-4 9.815977602657382E-5 0.0 6.0406016016353124E-5 5.134511361390015E-4 22-23 2.869285760776773E-4 9.815977602657382E-5 0.0 1.8499342405008142E-4 5.361033921451339E-4 24-25 2.9447932807972143E-4 1.0571052802861796E-4 0.0 5.512048961492221E-4 5.663064001533105E-4 26-27 2.9447932807972143E-4 1.0571052802861796E-4 0.0 0.0015554549124210926 5.96509408161487E-4 28-29 2.9447932807972143E-4 1.0571052802861796E-4 0.0 0.0038131297610322907 5.96509408161487E-4 30-31 2.9825470408074354E-4 1.0571052802861796E-4 0.0 0.01287780753948628 6.040601601635312E-4 32-33 3.020300800817656E-4 1.0571052802861796E-4 0.0 0.034435204505322295 8.532349762309878E-4 34-35 3.0958083208380973E-4 1.132612800306621E-4 0.0 0.0689043873946538 9.098656162463188E-4 36-37 3.133562080848318E-4 1.3591353603679453E-4 0.0 0.1261088845621402 9.778223842647161E-4 38-39 3.1713158408585386E-4 2.0387030405519176E-4 0.0 0.20854044416845607 0.0010080253922728926 40-41 3.3223308808994216E-4 2.6805169607256693E-4 0.0 0.2975562595205544 0.0010193515202759588 42-43 3.3223308808994216E-4 3.1713158408585386E-4 0.0 0.3876216294009369 0.0010571052802861795 44-45 3.3223308808994216E-4 3.1713158408585386E-4 0.0 0.49951999869523 0.0010684314082892456 46-47 3.3223308808994216E-4 3.209069600868759E-4 0.0 0.6336893110195523 0.001091083664295378 48-49 3.3223308808994216E-4 3.4355921609300835E-4 0.0 0.7828015615559201 0.0011477143043107092 50-51 3.9641448010731734E-4 3.6998684810016284E-4 0.0 0.9445499955677086 0.0011779173123188857 52-53 4.152913601124277E-4 3.7753760010220697E-4 0.0 1.0993404116096133 0.0011779173123188857 54-55 4.152913601124277E-4 3.7753760010220697E-4 0.0 1.2704706549839417 0.0012005695683250182 56-57 4.152913601124277E-4 3.7753760010220697E-4 0.0 1.47473359814324 0.001242098704336261 58-59 4.152913601124277E-4 3.8131297610322903E-4 0.0 1.7061226178698816 0.0012685263363434154 60-61 4.492697441216263E-4 4.039652321093615E-4 0.0 1.9461194948788534 0.0012760770883454597 62-63 5.889586561594429E-4 4.152913601124277E-4 0.0 2.1882494593284028 0.0012760770883454597 64-65 5.889586561594429E-4 4.1906673611344977E-4 0.0 2.4399688788205482 0.001291178592349548 66-67 6.342631681717077E-4 4.3039286411651595E-4 0.0 2.722140481136938 0.001291178592349548 68-69 6.720169281819283E-4 4.3039286411651595E-4 0.0 3.0311738837005997 0.0012987293443515921 70-71 7.399736962003257E-4 4.3039286411651595E-4 0.0 3.3663555404473398 0.001355359984366923 72-73 8.079304642187229E-4 4.3039286411651595E-4 0.0 3.7062677433233606 0.0013968891203781659 74-75 8.15481216220767E-4 4.3039286411651595E-4 0.0 4.039799560757654 0.001400664496379188 76-77 8.381334722268995E-4 4.605958721246925E-4 0.0 4.408529208649478 0.0014572951363945189 78-79 8.381334722268995E-4 4.605958721246925E-4 0.0 4.807133406837387 0.0014874981444026956 80-81 8.758872322371202E-4 4.605958721246925E-4 0.0 5.2307645725360725 0.0014950488964047396 82-83 9.287424962514291E-4 4.605958721246925E-4 0.0 5.666242868125965 0.0015516795364200705 84-85 9.362932482534733E-4 4.605958721246925E-4 0.0 6.086910363663849 0.0016007594244333576 86-87 9.362932482534733E-4 4.605958721246925E-4 0.0 6.523861055894139 0.0016083101764354018 88 9.362932482534733E-4 4.605958721246925E-4 0.0 6.870338637635938 0.001630962432441534 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 25135 0.0 51.327892 1 GTATCAA 45320 0.0 41.587364 1 CAACGCA 55295 0.0 33.11409 5 ATCAACG 55735 0.0 32.85813 3 TATCAAC 58630 0.0 31.881395 2 AACGCAG 60360 0.0 30.949392 6 TCAACGC 61640 0.0 29.900797 4 GTGGTAT 10180 0.0 28.72089 1 GTACTAG 7165 0.0 27.641006 1 ACGCAGA 67305 0.0 27.030907 7 CGCAGAG 68880 0.0 26.406013 8 CGTTAAC 2555 0.0 25.348122 1 GCAGAGT 74105 0.0 24.201494 9 TGGTATC 12225 0.0 24.024996 2 GTACATG 67615 0.0 23.876625 1 TACATGG 69090 0.0 23.020903 2 GAGTACT 34610 0.0 22.443468 12-13 ACATGGG 68650 0.0 22.432121 3 TAGTACT 9670 0.0 21.78682 4 CTGTGCG 9710 0.0 21.402763 9 >>END_MODULE