FastQCFastQC Report
Mon 6 Jun 2016
ERR1211324_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211324_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9010957
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335310.37211363898418337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264390.29340945695335136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154850.17184634218096922No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161500.051.105991
GTATCAA285550.040.0542561
CAACGCA343800.032.324065
ATCAACG347550.032.177913
TATCAAC363600.031.1577222
TCAACGC364450.030.7371834
AACGCAG370150.030.3283376
GTACATG377750.027.2650871
ACGCAGA414550.026.7404357
TACATGG388400.026.0928082
CGCAGAG423200.026.060058
GTGGTAT81050.025.7050231
ACATGGG386800.025.2682423
GCAGAGT457450.023.6180539
GTACTAG36450.023.611351
TACCTGG58150.023.5340182
TGGTATC85700.023.3766122
CATGGGG243450.022.4473824
CTGTGCG43250.021.07979
GAGTACT267650.021.04311212-13
Produced by FastQC (version 0.11.5)