##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211324_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9010957 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32525968107494 31.0 31.0 34.0 28.0 34.0 2 31.347478630738113 31.0 31.0 34.0 28.0 34.0 3 31.393935405529067 31.0 31.0 34.0 28.0 34.0 4 35.00268495344057 37.0 35.0 37.0 32.0 37.0 5 35.05054102466586 37.0 35.0 37.0 32.0 37.0 6 35.11633581205637 37.0 35.0 37.0 32.0 37.0 7 35.04681689192391 37.0 35.0 37.0 32.0 37.0 8 35.043253896339756 37.0 35.0 37.0 32.0 37.0 9 36.58961972629544 39.0 37.0 39.0 32.0 39.0 10-11 36.524024751200116 39.0 37.0 39.0 32.0 39.0 12-13 36.44271646174762 39.0 37.0 39.0 32.0 39.0 14-15 37.55894074291998 40.0 37.0 41.0 32.0 41.0 16-17 37.55554226926175 40.0 37.0 41.0 32.0 41.0 18-19 37.56737430885532 40.0 37.0 41.0 32.0 41.0 20-21 37.537007168051076 40.0 37.0 41.0 31.5 41.0 22-23 37.418203527105945 40.0 37.0 41.0 31.0 41.0 24-25 37.30700268573027 40.0 37.0 41.0 31.0 41.0 26-27 37.16043506810653 40.0 36.0 41.0 30.5 41.0 28-29 37.022177389149675 39.5 36.0 41.0 30.0 41.0 30-31 36.83889380450933 39.0 36.0 41.0 30.0 41.0 32-33 36.71913049856968 39.0 35.5 41.0 30.0 41.0 34-35 36.57583717245571 39.0 35.0 41.0 30.0 41.0 36-37 36.48057731270941 39.0 35.0 41.0 29.5 41.0 38-39 36.33211416944948 39.0 35.0 40.5 29.0 41.0 40-41 36.16804907625239 39.0 35.0 40.0 28.5 41.0 42-43 36.02118936978614 39.0 35.0 40.0 28.0 41.0 44-45 35.87934871956441 38.5 35.0 40.0 27.0 41.0 46-47 35.705772538921224 38.5 34.5 40.0 26.5 41.0 48-49 35.463889684525185 38.0 34.0 40.0 26.0 41.0 50-51 34.798954650432805 37.5 33.5 39.5 24.5 40.5 52-53 35.03973118504505 38.0 33.5 40.0 26.0 40.5 54-55 35.40512017757936 38.0 34.0 40.0 26.0 41.0 56-57 35.30696040387275 38.0 34.0 40.0 26.0 41.0 58-59 35.087823746134845 38.0 34.0 40.0 26.0 41.0 60-61 34.82831989987301 37.5 34.0 40.0 25.0 41.0 62-63 34.560940530511914 37.0 33.0 40.0 25.0 41.0 64-65 34.288843626709124 37.0 33.0 40.0 24.5 41.0 66-67 34.07158484942276 36.0 33.0 39.5 24.0 41.0 68-69 33.733999008096475 36.0 32.5 39.0 23.0 41.0 70-71 33.39638187153706 35.0 32.0 39.0 23.0 40.5 72-73 33.03445255592719 35.0 32.0 38.0 22.5 40.0 74-75 32.66052207329366 35.0 31.5 37.0 22.0 39.5 76-77 32.304733448400654 35.0 31.0 37.0 21.5 39.0 78-79 31.936568391126492 35.0 31.0 36.0 20.0 39.0 80-81 31.596250986437955 35.0 31.0 36.0 20.0 38.0 82-83 31.277070237933664 34.0 30.0 35.5 20.0 37.0 84-85 30.9663756025026 34.0 30.0 35.0 19.0 37.0 86-87 30.709398790827656 34.0 30.0 35.0 18.5 36.0 88-89 30.44223843261043 34.0 29.0 35.0 18.0 36.0 90-91 30.179318467505723 34.0 29.0 35.0 18.0 36.0 92-93 29.935332895274055 34.0 29.0 35.0 17.0 35.0 94-95 29.71132000740876 34.0 29.0 35.0 15.5 35.0 96-97 29.506717211057605 34.0 28.5 35.0 15.0 35.0 98-99 29.266734709753912 34.0 27.0 35.0 12.0 35.0 100 27.5749444814796 32.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.38813536518320646 1101 2 -0.377772322736881 1101 3 -0.3770368296570048 1101 4 -0.22713726517311272 1101 5 -0.34070583758570905 1101 6 -0.3243569161051596 1101 7 -0.419711093345704 1101 8 -0.4582108703925769 1101 9 -0.44484182053724197 1101 10-11 -0.5054026580573492 1101 12-13 -0.43542642408801413 1101 14-15 -0.5057942157727879 1101 16-17 -0.5826314873974852 1101 18-19 -0.6178605347410695 1101 20-21 -0.6844693159337325 1101 22-23 -0.7018232507343569 1101 24-25 -0.6686361286663427 1101 26-27 -0.6596590341493282 1101 28-29 -0.8147626770365051 1101 30-31 -0.8447230422642136 1101 32-33 -0.7945020617417242 1101 34-35 -0.7719745602118877 1101 36-37 -0.8461721396628406 1101 38-39 -0.8721648538300784 1101 40-41 -0.891163398047091 1101 42-43 -0.9070838120110452 1101 44-45 -0.9598080143823751 1101 46-47 -0.9401398381692374 1101 48-49 -0.9158819835024801 1101 50-51 -0.8561841455492001 1101 52-53 -0.6862032149106128 1101 54-55 -0.6697307941454156 1101 56-57 -0.6025754797588476 1101 58-59 -0.7206426547727744 1101 60-61 -0.6682053534218255 1101 62-63 -0.7178512782443036 1101 64-65 -0.7612925553148528 1101 66-67 -0.7620845578093878 1101 68-69 -0.7460739603896371 1101 70-71 -0.7845919975116615 1101 72-73 -0.8300860222462916 1101 74-75 -0.8575021619311727 1101 76-77 -0.7981594178702451 1101 78-79 -0.7712544679110813 1101 80-81 -0.8834620580817578 1101 82-83 -0.9074718272724986 1101 84-85 -0.9147298830670572 1101 86-87 -0.9423925749224651 1101 88-89 -1.0268315107224026 1101 90-91 -0.9789936662294991 1101 92-93 -0.949797004037066 1101 94-95 -1.0535638269459042 1101 96-97 -1.159732501180386 1101 98-99 -1.1508099123881799 1101 100 -1.2489701571891132 1102 1 -0.4604107413484293 1102 2 -0.4290900979446164 1102 3 -0.4283923071146205 1102 4 -0.3553463021087424 1102 5 -0.33461217807778354 1102 6 -0.2921836225635701 1102 7 -0.3335265384891386 1102 8 -0.32412955230530827 1102 9 -0.4082486723679324 1102 10-11 -0.4112176620458925 1102 12-13 -0.4292503150100444 1102 14-15 -0.607117351166643 1102 16-17 -0.5987608347101414 1102 18-19 -0.5303839955667442 1102 20-21 -0.5922972967341238 1102 22-23 -0.6311251842338734 1102 24-25 -0.6758210918106897 1102 26-27 -0.7023840854344741 1102 28-29 -0.71784726391774 1102 30-31 -0.7415050276842763 1102 32-33 -0.7575907730295839 1102 34-35 -0.7608454923289685 1102 36-37 -0.7916178443294584 1102 38-39 -0.8602157323488626 1102 40-41 -0.8775085834727463 1102 42-43 -0.7910165087785188 1102 44-45 -0.8819645112898513 1102 46-47 -0.8948847834870293 1102 48-49 -0.7897662223483337 1102 50-51 -0.8044637408661259 1102 52-53 -0.7841391546648424 1102 54-55 -0.6844360731960464 1102 56-57 -0.7588197065091364 1102 58-59 -0.7966248577090127 1102 60-61 -0.8225472109639185 1102 62-63 -0.8585032070985292 1102 64-65 -0.8662224329863264 1102 66-67 -0.7751572641807059 1102 68-69 -0.7700153785091928 1102 70-71 -0.8699667104139195 1102 72-73 -0.936476891763995 1102 74-75 -0.8523363604288008 1102 76-77 -0.8208911677565425 1102 78-79 -0.9183297284837586 1102 80-81 -1.0222094003846074 1102 82-83 -1.0158421242357072 1102 84-85 -1.117818658427975 1102 86-87 -1.129221313208813 1102 88-89 -1.1380085414438952 1102 90-91 -1.2396480817710618 1102 92-93 -1.180728846437745 1102 94-95 -1.2351146062107716 1102 96-97 -1.2407449955458105 1102 98-99 -1.2510231229124393 1102 100 -1.2018041531446784 1103 1 -0.43299357626667856 1103 2 -0.4319828820857339 1103 3 -0.4643699163434718 1103 4 -0.31932566153903963 1103 5 -0.31513067690738694 1103 6 -0.466408032567621 1103 7 -0.4211777613336949 1103 8 -0.45169964080287883 1103 9 -0.6828638943309002 1103 10-11 -0.6354618092617912 1103 12-13 -0.7222101158851402 1103 14-15 -0.7903590492145796 1103 16-17 -0.7005326133844889 1103 18-19 -0.7406575735472103 1103 20-21 -0.8901889081736059 1103 22-23 -0.8349996219643216 1103 24-25 -0.8378022970843162 1103 26-27 -0.7479615724098778 1103 28-29 -0.8516538639254634 1103 30-31 -0.9795311779772575 1103 32-33 -0.9644663915394247 1103 34-35 -1.0065827357848178 1103 36-37 -0.9693100383856645 1103 38-39 -1.0422088758917667 1103 40-41 -1.0229616023697972 1103 42-43 -1.070157513729093 1103 44-45 -1.076451750597954 1103 46-47 -1.036445117623444 1103 48-49 -1.0640131104474406 1103 50-51 -0.9975337911594906 1103 52-53 -0.8963868126736045 1103 54-55 -0.8573684319070551 1103 56-57 -0.9077099325826836 1103 58-59 -0.8821940828471355 1103 60-61 -0.8553772938169644 1103 62-63 -1.0183038449683366 1103 64-65 -0.9246921832524535 1103 66-67 -0.9688402182999738 1103 68-69 -0.9356282728009333 1103 70-71 -0.9486366648191691 1103 72-73 -1.0305517058490103 1103 74-75 -1.0029260917425233 1103 76-77 -1.038171940153795 1103 78-79 -0.9660575963623437 1103 80-81 -1.0312012615004598 1103 82-83 -1.1854734040506933 1103 84-85 -1.0925929687294946 1103 86-87 -1.1494129816475542 1103 88-89 -1.2034528973182468 1103 90-91 -1.1214208090567581 1103 92-93 -1.1695983156698766 1103 94-95 -1.3690598569223091 1103 96-97 -1.4712155645785394 1103 98-99 -1.3050450676050325 1103 100 -1.4742169692232778 1104 1 -0.3821935675242294 1104 2 -0.4167130697634569 1104 3 -0.4573731667214318 1104 4 -0.330351796227383 1104 5 -0.28517890502822496 1104 6 -0.2659070703326023 1104 7 -0.3192679419872917 1104 8 -0.32559041808185896 1104 9 -0.44124037389724435 1104 10-11 -0.39802600634198626 1104 12-13 -0.5031316004730328 1104 14-15 -0.5642103874299593 1104 16-17 -0.5768908925779144 1104 18-19 -0.6476781220580463 1104 20-21 -0.6283631885916989 1104 22-23 -0.5831792400125195 1104 24-25 -0.678502644472978 1104 26-27 -0.733842268480764 1104 28-29 -0.6717739645999856 1104 30-31 -0.7316175816696671 1104 32-33 -0.7861828715368517 1104 34-35 -0.7984348382253827 1104 36-37 -0.8480713597703229 1104 38-39 -0.783201208405039 1104 40-41 -0.8527900598646809 1104 42-43 -0.8663339231356204 1104 44-45 -0.898751792079338 1104 46-47 -0.9688318681796062 1104 48-49 -0.8880048670843266 1104 50-51 -0.8759079167807258 1104 52-53 -0.8055813213477521 1104 54-55 -0.7705738256239769 1104 56-57 -0.7356900997127838 1104 58-59 -0.7764431895177282 1104 60-61 -0.763489630303873 1104 62-63 -0.8129074129946403 1104 64-65 -0.7820585404174238 1104 66-67 -0.7724873339034559 1104 68-69 -0.671649114280342 1104 70-71 -0.7583901683337118 1104 72-73 -0.7554052528601005 1104 74-75 -0.7888372732577977 1104 76-77 -0.8257595646893847 1104 78-79 -0.7830538779405778 1104 80-81 -0.8823331333636091 1104 82-83 -0.9474733089335743 1104 84-85 -0.9599323318487265 1104 86-87 -0.95935085942833 1104 88-89 -0.8494960424501556 1104 90-91 -0.8904526309524954 1104 92-93 -0.8523693697110204 1104 94-95 -0.9456248175036812 1104 96-97 -1.0765641710124179 1104 98-99 -1.0654014116944914 1104 100 -1.1959990899890478 1105 1 -0.44516768147783736 1105 2 -0.43667188308935323 1105 3 -0.41826505431542316 1105 4 -0.30080338463899636 1105 5 -0.3730077184049563 1105 6 -0.3479014878104749 1105 7 -0.3464973055631688 1105 8 -0.387958728237777 1105 9 -0.5337863205403863 1105 10-11 -0.49892849704614406 1105 12-13 -0.5383001716586406 1105 14-15 -0.5806351920963948 1105 16-17 -0.6353987561946752 1105 18-19 -0.6849302378479578 1105 20-21 -0.6801454338691286 1105 22-23 -0.6652043016533824 1105 24-25 -0.6126497311134429 1105 26-27 -0.7051903063597109 1105 28-29 -0.7847093511669101 1105 30-31 -0.7791536861371426 1105 32-33 -0.7378953460576057 1105 34-35 -0.7391025475403126 1105 36-37 -0.8136624378558182 1105 38-39 -0.7804762257526008 1105 40-41 -0.8151106130886134 1105 42-43 -0.925140782741046 1105 44-45 -0.9087178583358124 1105 46-47 -0.8504940406083676 1105 48-49 -0.8637183914333235 1105 50-51 -0.802853165598556 1105 52-53 -0.6642098316373435 1105 54-55 -0.6398102063972644 1105 56-57 -0.6679797322008127 1105 58-59 -0.6237491985394072 1105 60-61 -0.5883585906071644 1105 62-63 -0.6392285102888806 1105 64-65 -0.6073570862212136 1105 66-67 -0.6398586928233243 1105 68-69 -0.6947856799209333 1105 70-71 -0.6370233720339016 1105 72-73 -0.6978214601012667 1105 74-75 -0.7217334706216008 1105 76-77 -0.7014423343036711 1105 78-79 -0.6475923318991867 1105 80-81 -0.6716270852426227 1105 82-83 -0.658697787651807 1105 84-85 -0.6240941971896561 1105 86-87 -0.6619820485404695 1105 88-89 -0.6931998114438294 1105 90-91 -0.7314343180064355 1105 92-93 -0.749209392159667 1105 94-95 -0.7840637713872063 1105 96-97 -0.869255891393248 1105 98-99 -0.9442938989031369 1105 100 -0.9378149660147699 1106 1 -0.4551591395473622 1106 2 -0.4888745380322277 1106 3 -0.46479256953386283 1106 4 -0.3457962387383091 1106 5 -0.3113353233008098 1106 6 -0.31623055875193273 1106 7 -0.3910684643593427 1106 8 -0.3657518237630839 1106 9 -0.519875090888199 1106 10-11 -0.5333748390622901 1106 12-13 -0.4846044507114158 1106 14-15 -0.6015298285699515 1106 16-17 -0.6763454815831764 1106 18-19 -0.6772979129425281 1106 20-21 -0.6368864573404309 1106 22-23 -0.6681181316075211 1106 24-25 -0.7338654687272879 1106 26-27 -0.6913086119539145 1106 28-29 -0.7942302743241143 1106 30-31 -0.7744463794286816 1106 32-33 -0.8298653312742701 1106 34-35 -0.8381367006874925 1106 36-37 -0.8507059811251665 1106 38-39 -0.8013095626581688 1106 40-41 -0.914495788423956 1106 42-43 -0.8492735081655596 1106 44-45 -0.789354725124852 1106 46-47 -0.8200610627553715 1106 48-49 -0.8724907739917853 1106 50-51 -0.88929794497583 1106 52-53 -0.6711189359520588 1106 54-55 -0.7632020215189144 1106 56-57 -0.697232786248172 1106 58-59 -0.7392267107428765 1106 60-61 -0.7610050801423611 1106 62-63 -0.7673577532120746 1106 64-65 -0.864785393590104 1106 66-67 -0.8450260749373939 1106 68-69 -0.760813645098203 1106 70-71 -0.8336473804883866 1106 72-73 -0.7792550640957643 1106 74-75 -0.728420138951499 1106 76-77 -0.6866632513737834 1106 78-79 -0.6764567409699218 1106 80-81 -0.8467602796302707 1106 82-83 -0.8193511810329959 1106 84-85 -0.7472952406254727 1106 86-87 -0.7952672281339126 1106 88-89 -0.9137222066480035 1106 90-91 -0.7460541506600435 1106 92-93 -0.8380344523375491 1106 94-95 -0.8754914197500234 1106 96-97 -0.9454589275651344 1106 98-99 -0.9078165978902497 1106 100 -0.9341153013191175 1107 1 -0.46644197552681277 1107 2 -0.5675627528940872 1107 3 -0.5296544641223839 1107 4 -0.4219069888211848 1107 5 -0.3489906836704293 1107 6 -0.418762900768634 1107 7 -0.43353258242041903 1107 8 -0.39071260087116855 1107 9 -0.5436064111373113 1107 10-11 -0.5320561747791075 1107 12-13 -0.5242251080780918 1107 14-15 -0.6785764953335089 1107 16-17 -0.6420057099514267 1107 18-19 -0.7025663829971407 1107 20-21 -0.6886519546216476 1107 22-23 -0.7111372873274249 1107 24-25 -0.7748233723735822 1107 26-27 -0.7961837042583397 1107 28-29 -0.7760261464218488 1107 30-31 -0.7577937617202153 1107 32-33 -0.8218977379992154 1107 34-35 -0.7776166608521251 1107 36-37 -0.8519276522314456 1107 38-39 -0.8860463384707131 1107 40-41 -0.8041883169388413 1107 42-43 -0.8779671557526356 1107 44-45 -0.8311848406930977 1107 46-47 -0.7568453877167727 1107 48-49 -0.7773025075709441 1107 50-51 -0.7672266510114198 1107 52-53 -0.6896163618091933 1107 54-55 -0.6215573798815299 1107 56-57 -0.6479272407852577 1107 58-59 -0.6442433999363217 1107 60-61 -0.600827371202179 1107 62-63 -0.6517520943193915 1107 64-65 -0.6676648417636386 1107 66-67 -0.5918688657110351 1107 68-69 -0.5147331314184598 1107 70-71 -0.555457592212413 1107 72-73 -0.66409551153086 1107 74-75 -0.6587955783274779 1107 76-77 -0.5580013350543638 1107 78-79 -0.5889977545849554 1107 80-81 -0.539400667132373 1107 82-83 -0.6496556489477356 1107 84-85 -0.6097655250026861 1107 86-87 -0.7122900324305412 1107 88-89 -0.7041653509294186 1107 90-91 -0.6660202809056486 1107 92-93 -0.6323511989033399 1107 94-95 -0.6798183604613399 1107 96-97 -0.7645582094073085 1107 98-99 -0.8005379226170426 1107 100 -0.7122585826989898 1108 1 -0.5493336879634576 1108 2 -0.5021452442181946 1108 3 -0.515394411070222 1108 4 -0.41261315592210224 1108 5 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2311 18-19 0.7806035721308504 2311 20-21 0.846150821511543 2311 22-23 0.9259028538706886 2311 24-25 0.9329570061945063 2311 26-27 0.9810450951845269 2311 28-29 0.997799257034039 2311 30-31 0.9422367068623387 2311 32-33 1.0050793338476325 2311 34-35 1.0347948025763074 2311 36-37 1.0482297194222383 2311 38-39 1.06672501183116 2311 40-41 1.0589443629263755 2311 42-43 1.0568607193852912 2311 44-45 1.0717034278354092 2311 46-47 1.1266158488483882 2311 48-49 1.1512926622451758 2311 50-51 1.1050814919058425 2311 52-53 1.062582427225287 2311 54-55 0.9965956540919692 2311 56-57 1.021341380251279 2311 58-59 0.9674478136910736 2311 60-61 1.0177017176056111 2311 62-63 0.9949835152679398 2311 64-65 0.982361033902194 2311 66-67 0.9651574363920332 2311 68-69 0.9525165954091577 2311 70-71 0.973148875508322 2311 72-73 0.9642305703580405 2311 74-75 0.9550233347001154 2311 76-77 1.0366472855843512 2311 78-79 0.9839790209014332 2311 80-81 0.9977857657268991 2311 82-83 0.9925674926028307 2311 84-85 1.0536890557444778 2311 86-87 1.0726558002071478 2311 88-89 1.053706506023687 2311 90-91 1.1091993968481617 2311 92-93 1.0923934807446294 2311 94-95 1.1173780382449543 2311 96-97 1.118818650472523 2311 98-99 1.1252664859572086 2311 100 1.2398103195447447 2312 1 0.5109661472753473 2312 2 0.47524230012556146 2312 3 0.49941618983187297 2312 4 0.4604310723011764 2312 5 0.4498331153562347 2312 6 0.49221173232887594 2312 7 0.4913977495428554 2312 8 0.49683828993858015 2312 9 0.621514448597253 2312 10-11 0.6053659902215927 2312 12-13 0.6290796442796989 2312 14-15 0.8148984296028274 2312 16-17 0.7823125805607845 2312 18-19 0.776670800074946 2312 20-21 0.7897291678855041 2312 22-23 0.7695866279370662 2312 24-25 0.8340073199314659 2312 26-27 0.8544379690052466 2312 28-29 0.8860878967268633 2312 30-31 0.949457522596866 2312 32-33 0.8903175098782725 2312 34-35 0.8784872557154557 2312 36-37 0.8601600688647508 2312 38-39 0.9894095465837083 2312 40-41 0.9385194055690391 2312 42-43 0.9905908527573004 2312 44-45 0.9620855482081581 2312 46-47 0.9936869014217535 2312 48-49 0.9730133514034875 2312 50-51 0.8998376460596873 2312 52-53 0.8389762363889091 2312 54-55 0.7998648457492479 2312 56-57 0.7989875872967858 2312 58-59 0.7821079043343886 2312 60-61 0.8203017373908281 2312 62-63 0.828534260654358 2312 64-65 0.8160214581905691 2312 66-67 0.8400831650347556 2312 68-69 0.8463871269923757 2312 70-71 0.902608674657678 2312 72-73 0.8870523765514307 2312 74-75 0.8366748455039499 2312 76-77 0.8322432168350318 2312 78-79 0.829023796468288 2312 80-81 0.9232182062883076 2312 82-83 0.9448972245561933 2312 84-85 0.9616493707226219 2312 86-87 0.9265483144223943 2312 88-89 0.9446087563152403 2312 90-91 1.0301363657349896 2312 92-93 1.0247828125819858 2312 94-95 1.0186292379539488 2312 96-97 1.0824421794849002 2312 98-99 1.1800903645288372 2312 100 1.365725424080722 2313 1 0.4868482029916166 2313 2 0.4979427898260447 2313 3 0.5384559811733531 2313 4 0.3796607145140527 2313 5 0.3903577028055025 2313 6 0.4134830058509422 2313 7 0.43817035541902527 2313 8 0.43516014456164953 2313 9 0.5271119019110913 2313 10-11 0.6102185788554451 2313 12-13 0.6013835834138987 2313 14-15 0.7629197461404473 2313 16-17 0.7098183690479019 2313 18-19 0.7123284071047138 2313 20-21 0.7095490801157283 2313 22-23 0.6556624512336882 2313 24-25 0.7410968000268454 2313 26-27 0.7619522464838369 2313 28-29 0.7945063233337564 2313 30-31 0.8449870382221931 2313 32-33 0.9128299616399431 2313 34-35 0.8916589403744553 2313 36-37 0.8863345767578039 2313 38-39 0.8963947637851746 2313 40-41 0.9161838007463032 2313 42-43 0.8183679729352278 2313 44-45 0.8895074723479723 2313 46-47 0.8678061260197865 2313 48-49 0.8989794350314426 2313 50-51 0.8196513682240436 2313 52-53 0.6785844995036214 2313 54-55 0.7139662181409037 2313 56-57 0.7519794327286675 2313 58-59 0.7483007444106917 2313 60-61 0.7614260233100936 2313 62-63 0.7279463012189282 2313 64-65 0.6831156207394287 2313 66-67 0.7417725670253219 2313 68-69 0.7468217833597777 2313 70-71 0.7128481772835826 2313 72-73 0.7405420527435922 2313 74-75 0.7256505854090562 2313 76-77 0.8092140097259772 2313 78-79 0.7454141536974142 2313 80-81 0.7425807943837093 2313 82-83 0.7770227602577506 2313 84-85 0.8287530933276521 2313 86-87 0.8218483792991726 2313 88-89 0.8287971547349144 2313 90-91 0.8514842917310936 2313 92-93 0.89510661967498 2313 94-95 0.9234682319435059 2313 96-97 0.9991014121190247 2313 98-99 0.9887532146054987 2313 100 1.0538228006885006 2314 1 0.3038022757602228 2314 2 0.2075082357166309 2314 3 0.3258940462838211 2314 4 0.23927061602953614 2314 5 0.2070984345250011 2314 6 0.23571109728194983 2314 7 0.3482463464978309 2314 8 0.3193154808052441 2314 9 0.3780311916826946 2314 10-11 0.42959023877453006 2314 12-13 0.43882423186565944 2314 14-15 0.5330095371029628 2314 16-17 0.5568518293942191 2314 18-19 0.5640365481982386 2314 20-21 0.5889477178310187 2314 22-23 0.6349432335184773 2314 24-25 0.6592464597392151 2314 26-27 0.6638601837491365 2314 28-29 0.6148544151480522 2314 30-31 0.648381623442198 2314 32-33 0.6752892726469994 2314 34-35 0.7192921002217005 2314 36-37 0.7539760439596321 2314 38-39 0.803038329870887 2314 40-41 0.826313519124362 2314 42-43 0.7269890327619564 2314 44-45 0.7893455729314027 2314 46-47 0.6710372819673296 2314 48-49 0.604540118420438 2314 50-51 0.789139882967639 2314 52-53 0.6789387596093661 2314 54-55 0.7210452160429881 2314 56-57 0.6739876297111707 2314 58-59 0.6347148654802552 2314 60-61 0.5140440028932218 2314 62-63 0.6748526020945533 2314 64-65 0.6952129790548227 2314 66-67 0.7141526689682394 2314 68-69 0.6988091614468814 2314 70-71 0.705868975583364 2314 72-73 0.7674260427040451 2314 74-75 0.7696355927118361 2314 76-77 0.7348584271062961 2314 78-79 0.7758089213422501 2314 80-81 0.8086161854938716 2314 82-83 0.7732181554224908 2314 84-85 0.7999299517536151 2314 86-87 0.8460689111086275 2314 88-89 0.7114617703978752 2314 90-91 0.7564347914565168 2314 92-93 0.9356824370130319 2314 94-95 0.974511573153169 2314 96-97 0.9425104377929117 2314 98-99 0.9095883047735001 2314 100 1.0268774706178512 2315 1 0.2924552491244441 2315 2 0.2853859189929402 2315 3 0.3066889249393796 2315 4 0.2361809446341212 2315 5 0.2247331216665529 2315 6 0.2042789132474141 2315 7 0.20658391776655805 2315 8 0.15469026319802737 2315 9 0.13050119065078292 2315 10-11 0.2536583447176639 2315 12-13 0.22926033370226406 2315 14-15 0.4057841738667278 2315 16-17 0.23862416275295573 2315 18-19 0.3981207355987024 2315 20-21 0.25404958474036476 2315 22-23 0.2306016420359498 2315 24-25 0.38004486091200107 2315 26-27 0.4775770280844185 2315 28-29 0.41522499047830763 2315 30-31 0.45050779047857503 2315 32-33 0.4552592427935238 2315 34-35 0.41929655494600837 2315 36-37 0.5670015825549228 2315 38-39 0.6036207945920111 2315 40-41 0.6179786724007883 2315 42-43 0.5802939786428567 2315 44-45 0.5836125915676291 2315 46-47 0.6739331228273997 2315 48-49 0.5592495004771294 2315 50-51 0.5325767219809805 2315 52-53 0.24473491358582322 2315 54-55 0.4114012479513107 2315 56-57 0.39333913979540824 2315 58-59 0.4437200485975765 2315 60-61 0.47178779509633983 2315 62-63 0.45328557282047655 2315 64-65 0.49812544344780463 2315 66-67 0.3541895350658919 2315 68-69 0.4996960190468158 2315 70-71 0.44754802696208174 2315 72-73 0.4981695516855069 2315 74-75 0.2474694787255416 2315 76-77 0.45298086804162097 2315 78-79 0.5371115246987195 2315 80-81 0.570314923725757 2315 82-83 0.5188497010049815 2315 84-85 0.5095618572912848 2315 86-87 0.4946190953620828 2315 88-89 0.3891557332644737 2315 90-91 0.5710592360614868 2315 92-93 0.690346646695442 2315 94-95 0.6720156578015342 2315 96-97 0.6496327113949434 2315 98-99 0.8149068055189055 2315 100 0.7947384313673851 2316 1 0.06163645732584655 2316 2 0.10717917235540497 2316 3 0.11318485572578396 2316 4 0.0014881218032485322 2316 5 -0.07531562276977155 2316 6 -0.16283794386240658 2316 7 -0.08521479220255657 2316 8 0.018014737898745636 2316 9 0.02129261627479906 2316 10-11 0.02333186599800996 2316 12-13 0.03774086497514162 2316 14-15 -0.11688773861984458 2316 16-17 0.04123520987068474 2316 18-19 0.11683313160410336 2316 20-21 0.13779256799671913 2316 22-23 0.1191188914097765 2316 24-25 0.11297043105814453 2316 26-27 -0.013802272833615348 2316 28-29 0.0490151811842523 2316 30-31 -0.5959795665080989 2316 32-33 -0.4000052118723829 2316 34-35 -0.44070916097833646 2316 36-37 -0.2606185385124249 2316 38-39 -0.07833531444770614 2316 40-41 0.10606225198612407 2316 42-43 0.08073710988213634 2316 44-45 0.018639727671526884 2316 46-47 -0.044476219489993696 2316 48-49 0.01923028007185934 2316 50-51 0.12955006184829188 2316 52-53 -0.2064903963626108 2316 54-55 -0.1344655126737564 2316 56-57 -0.14208661655089827 2316 58-59 -0.5308971275823566 2316 60-61 -0.10078459598064171 2316 62-63 -0.05798724416411716 2316 64-65 -0.38339586497694 2316 66-67 -0.5551360151957567 2316 68-69 -0.6598673128689541 2316 70-71 -0.4309421804710709 2316 72-73 -0.6889968035135254 2316 74-75 -0.4992018558331779 2316 76-77 -0.1289493465468965 2316 78-79 -0.05346462645010419 2316 80-81 -0.1497783520154421 2316 82-83 0.09057549531686249 2316 84-85 0.06252040573984274 2316 86-87 0.18219301240392483 2316 88-89 0.20704120871209497 2316 90-91 -0.3756380120414491 2316 92-93 -0.43626001864755537 2316 94-95 -0.060482476421547915 2316 96-97 0.2333706601735912 2316 98-99 0.2512449670040162 2316 100 0.2639487164764276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 250.0 1 667.0 2 421.0 3 393.0 4 347.0 5 339.0 6 378.0 7 957.0 8 2238.0 9 3874.0 10 6414.0 11 10598.0 12 16064.0 13 21123.0 14 25217.0 15 29361.0 16 34722.0 17 41909.0 18 50713.0 19 60697.0 20 73630.0 21 88626.0 22 99382.0 23 101358.0 24 101322.0 25 105184.0 26 115054.0 27 130614.0 28 152377.0 29 180875.0 30 217841.0 31 266035.0 32 334935.0 33 432058.0 34 588737.0 35 812902.0 36 1193321.0 37 1712359.0 38 1607775.0 39 389549.0 40 341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94954827510456 19.490090180693286 13.446015630905755 23.114345913296393 2 15.614793332817104 20.079994107428117 40.9900031807001 23.315209379054686 3 19.063180682793938 25.941612123246376 29.38567281761941 25.609534376340275 4 12.549042024604399 16.61492236134289 38.599424043758916 32.236611570293796 5 13.208931922577987 37.81308797737061 34.81144598353078 14.166534116520623 6 30.58483017488681 37.27889893629895 18.106399859484785 14.02987102932944 7 27.560893720026673 32.413916305956036 22.271354465169377 17.753835508847914 8 25.845353348707228 35.464807233458075 20.20208546767152 18.487753950163174 9 26.47819099411376 16.182289139386054 20.870224345087056 36.46929552141313 10-11 24.89373570341039 26.429442477620178 28.013885812015758 20.662936006953675 12-13 25.390872618944798 24.133066212712595 28.335514438479404 22.14054672986321 14-15 22.94894711107675 25.607128284781034 26.053481452017962 25.390443152124252 16-17 21.947820817861572 28.517587946672094 26.991038317419854 22.54355291804648 18-19 21.638124301557827 27.69918133085304 29.21236816331551 21.45032620427362 20-21 22.57990250954239 26.87814303467293 29.164852472194458 21.377101983590226 22-23 22.63187420040809 26.66407965982526 28.825807003182963 21.87823913658369 24-25 22.196538910809068 26.843505603393492 29.14914562193256 21.810809863864876 26-27 22.069231485461053 27.05299498910858 28.88146427673761 21.996309248692754 28-29 21.838613414391226 27.064080474000352 29.174062466481566 21.923243645126856 30-31 22.400403978988738 26.90726681410982 29.015187962261095 21.677141244640342 32-33 22.23448134820705 27.181047010038036 28.71507169146128 21.869399950293637 34-35 22.386718846281013 27.112933528947675 28.91631336102637 21.584034263744943 36-37 22.351426969224857 26.912093020312444 28.731135863306363 22.005344147156336 38-39 22.21490795036986 27.152496665138 28.891754395830816 21.74084098866133 40-41 22.227034489987584 26.713284531349274 29.167232035645895 21.892448943017246 42-43 22.055294997800026 26.830546468035138 28.81043440121144 22.30372413295339 44-45 22.029621474997505 27.223012536365033 28.7392034766074 22.008162512030065 46-47 22.09691505018416 26.99945027315056 28.404765528256632 22.498869148408648 48-49 22.32795446776126 27.044435952008573 28.34584406832812 22.281765511902055 50-51 22.258318901138995 27.16430449481657 28.17515215291475 22.402224451129687 52-53 22.107136555110138 27.417173799845884 28.065033868289845 22.410655776754126 54-55 22.053027623487985 27.32980795905679 28.095350213388386 22.52181420406684 56-57 22.304162100160006 27.273354142168536 27.83985635907566 22.582627398595793 58-59 22.0365586736688 27.25603640944009 28.23809107156816 22.46931384532295 60-61 22.25065954813798 27.540074339725983 27.903190141672734 22.3060759704633 62-63 22.179392909482296 27.289531138578425 28.021406044610934 22.50966990732834 64-65 21.924220506871443 27.116992049407436 28.075593504614876 22.88319393910625 66-67 22.088425742768845 27.096107730143448 27.857772031570043 22.957694495517668 68-69 21.855655896536483 27.124128338121388 28.062380945216077 22.957834820126056 70-71 21.99078776691044 26.853635581843616 28.114655829912422 23.040920821333525 72-73 22.07934979399009 27.085372852175677 27.91776304685015 22.91751430698408 74-75 22.09623894265908 27.214820049904638 27.81767847680106 22.87126253063522 76-77 21.972387621481268 27.04707641885286 27.840896630877655 23.139639328788213 78-79 21.888736772069244 26.970878466582505 27.846128552908194 23.294256208440057 80-81 21.82151848193671 27.29871331342517 27.76479482458133 23.11497338005679 82-83 22.20223495199317 26.909052954638067 28.075640599999335 22.813071493369428 84-85 22.155964637319766 27.026850256723 27.665443034010828 23.1517420719464 86-87 21.738151103498158 27.265121989663992 27.95802192644293 23.038704980394918 88-89 21.995967334454047 26.74880804605283 27.965581642654353 23.28964297683877 90-91 22.138961101484103 26.723695382609712 27.92163380641136 23.215709709494824 92-93 22.16805710539782 26.772283924377383 27.824937715545307 23.234721254679492 94-95 21.766938629538142 26.583952339487855 28.038379862456242 23.61072916851776 96-97 21.938295995063136 26.521669379597597 27.89697626064661 23.64305836469266 98-99 21.74039374831623 26.618927178259245 27.8246044465 23.81607462692452 100 22.10625364682074 26.38834208570311 27.445782588979096 24.059621678497063 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2697.0 1 1935.5 2 1929.5 3 2570.0 4 1991.0 5 1703.0 6 2490.5 7 4458.5 8 7239.5 9 8801.0 10 7850.0 11 6601.5 12 6733.0 13 7394.5 14 8294.5 15 9087.0 16 9690.5 17 10581.0 18 11479.0 19 12274.5 20 13380.5 21 14868.0 22 16978.0 23 19967.0 24 23578.0 25 28280.0 26 34951.0 27 43254.5 28 52232.5 29 65932.0 30 85857.5 31 105896.0 32 131668.5 33 161490.0 34 190546.5 35 218268.0 36 250637.5 37 285727.5 38 300294.5 39 303123.0 40 298853.5 41 290055.0 42 290237.0 43 300031.0 44 313579.0 45 332256.0 46 345918.0 47 351727.0 48 358940.5 49 360286.5 50 352131.5 51 341919.0 52 330782.5 53 317948.5 54 304052.0 55 278819.5 56 250313.0 57 217801.5 58 185274.5 59 160020.0 60 131909.0 61 104318.0 62 82716.5 63 64902.0 64 48211.5 65 33744.5 66 24114.0 67 17899.0 68 13400.5 69 9975.5 70 7405.5 71 5515.5 72 4157.5 73 2976.5 74 2168.0 75 1615.0 76 1137.0 77 739.5 78 465.0 79 303.5 80 234.5 81 160.5 82 110.0 83 95.0 84 71.5 85 55.5 86 46.0 87 41.0 88 27.0 89 18.5 90 23.0 91 18.5 92 15.5 93 13.5 94 7.5 95 7.0 96 5.5 97 3.0 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025147162504493142 2 0.10868989830935827 3 0.017989210247035915 4 0.01766737983546032 5 0.019842509513695383 6 0.04553345443774729 7 0.046598824076066504 8 0.03863074698947071 9 0.03333719159907211 10-11 0.037127022135384734 12-13 0.0365222029136306 14-15 0.03279895797971292 16-17 0.03942422541801054 18-19 0.037853914961529615 20-21 0.049212308970068334 22-23 0.04893486896008937 24-25 0.06869969527099064 26-27 0.060742715784794 28-29 0.05236957628362891 30-31 0.05047188661537282 32-33 0.04507290402118221 34-35 0.04571101604413383 36-37 0.041283073484869585 38-39 0.04674864168145514 40-41 0.055920808411359636 42-43 0.05736904526344982 44-45 0.05988265175385922 46-47 0.061874671025508164 48-49 0.061674914218323315 50-51 0.05604288201575038 52-53 0.06576437996541322 54-55 0.052452808286622604 56-57 0.05734130126245192 58-59 0.054833243572242105 60-61 0.0510656082367278 62-63 0.057518862868838457 64-65 0.055759893205571835 66-67 0.053534824325540555 68-69 0.05977722455006721 70-71 0.06027661656802934 72-73 0.061758146221317006 74-75 0.053040981107778 76-77 0.054239521950887125 78-79 0.06454919272170537 80-81 0.0640608983041424 82-83 0.05489982917463705 84-85 0.06138082780774561 86-87 0.058323438897777455 88-89 0.06653011439295516 90-91 0.07199568258954071 92-93 0.07662338195598979 94-95 0.08075723810467633 96-97 0.07610179473722936 98-99 0.08565682868090481 100 0.12961997266217118 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9010957.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.55158148338916 #Duplication Level Percentage of deduplicated Percentage of total 1 72.90034736019217 39.76829239186897 2 15.251837366122775 16.64023697698892 3 5.45853147500324 8.933145736148509 4 2.4271290958193177 5.296149225651686 5 1.2250448338409672 3.341406653704023 6 0.7143683660235735 2.338195447697432 7 0.4570538924368493 1.7453108858898274 8 0.29824449387987706 1.3015767047888216 9 0.2114596467102307 1.038191233316565 >10 0.9555680558504476 8.995123995266287 >50 0.0553501361235474 2.092192122023078 >100 0.03937476807316985 4.314007321281217 >500 0.003974349711929808 1.437901728051981 >1k 0.0015957279164886583 1.4091832845550538 >5k 3.0108073870279147E-5 0.10978089313063621 >10k+ 9.032422161083744E-5 1.2393053996367678 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33531 0.37211363898418337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26439 0.29340945695335136 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15485 0.17184634218096922 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.1097600399158491E-5 7 0.0 0.0 0.0 0.0 1.1097600399158491E-5 8 0.0 0.0 0.0 0.0 4.4390401596633966E-5 9 0.0 0.0 0.0 0.0 5.5488001995792455E-5 10-11 0.0 0.0 0.0 1.1097600399158491E-5 6.10368021953717E-5 12-13 0.0 0.0 0.0 1.1097600399158491E-5 6.658560239495095E-5 14-15 0.0 0.0 0.0 1.1097600399158491E-5 7.768320279410943E-5 16-17 0.0 0.0 0.0 1.1097600399158491E-5 1.331712047899019E-4 18-19 0.0 0.0 0.0 2.2195200798316983E-5 1.442688051890604E-4 20-21 0.0 0.0 0.0 6.658560239495095E-5 1.4981760538863961E-4 22-23 0.0 0.0 0.0 4.161600149684434E-4 1.7756160638653584E-4 24-25 0.0 0.0 0.0 0.001082016038917953 1.9975680718485283E-4 26-27 0.0 0.0 0.0 0.002213971279632119 1.9975680718485283E-4 28-29 1.1097600399158491E-5 0.0 0.0 0.00566532500377041 1.9975680718485283E-4 30-31 1.1097600399158491E-5 0.0 0.0 0.0185274438663951 2.0530560738443208E-4 32-33 1.1097600399158491E-5 0.0 0.0 0.04755321771039414 2.1085440758401134E-4 34-35 2.2195200798316983E-5 0.0 0.0 0.0927149025347696 2.1085440758401134E-4 36-37 2.2195200798316983E-5 0.0 0.0 0.1665638843909698 2.1085440758401134E-4 38-39 2.2195200798316983E-5 0.0 0.0 0.2724516385995405 2.441472087814868E-4 40-41 2.2195200798316983E-5 0.0 0.0 0.38512002665199707 2.552448091806453E-4 42-43 2.2195200798316983E-5 0.0 0.0 0.4939209009653469 2.552448091806453E-4 44-45 2.2195200798316983E-5 0.0 0.0 0.6294392482396709 2.663424095798038E-4 46-47 2.2195200798316983E-5 0.0 0.0 0.7942774557685714 2.996352107772793E-4 48-49 2.2195200798316983E-5 0.0 0.0 0.9675664860014315 3.3292801197475476E-4 50-51 2.2195200798316983E-5 0.0 0.0 1.143546684331087 3.3292801197475476E-4 52-53 2.2195200798316983E-5 0.0 0.0 1.3151433305030753 3.4402561237391327E-4 54-55 2.2195200798316983E-5 0.0 0.0 1.5081694430458386 3.60672012972651E-4 56-57 2.2195200798316983E-5 0.0 0.0 1.7326239599190187 3.662208131722302E-4 58-59 3.3292801197475476E-5 0.0 0.0 1.9829358857222381 3.773184135713887E-4 60-61 3.3292801197475476E-5 0.0 0.0 2.238064170098692 3.939648141701264E-4 62-63 3.3292801197475476E-5 0.0 0.0 2.493575321688917 4.1061121476886417E-4 64-65 3.3292801197475476E-5 0.0 0.0 2.761105174511431 4.1061121476886417E-4 66-67 3.3292801197475476E-5 0.0 0.0 3.0574943371719563 4.1061121476886417E-4 68-69 3.3292801197475476E-5 0.0 0.0 3.3857447105784657 4.161600149684434E-4 70-71 3.3292801197475476E-5 0.0 0.0 3.738104620852147 4.3280641556718113E-4 72-73 3.3292801197475476E-5 0.0 0.0 4.082651820444821 5.65977620357083E-4 74-75 3.3292801197475476E-5 0.0 0.0 4.422071928653083 5.65977620357083E-4 76-77 3.3292801197475476E-5 0.0 0.0 4.7966603325262795 5.65977620357083E-4 78-79 3.3292801197475476E-5 0.0 0.0 5.204053243179387 5.65977620357083E-4 80-81 3.3292801197475476E-5 5.548800199579246E-6 0.0 5.632193117778723 5.937216213549793E-4 82-83 3.3292801197475476E-5 1.6646400598737738E-5 0.0 6.062979770073256 6.214656223528755E-4 84-85 3.3292801197475476E-5 2.2195200798316983E-5 0.0 6.491902025500732 6.214656223528755E-4 86-87 3.3292801197475476E-5 2.2195200798316983E-5 0.0 6.932826335759898 6.214656223528755E-4 88 3.3292801197475476E-5 2.2195200798316983E-5 0.0 7.284331730802844 6.214656223528755E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16150 0.0 51.10599 1 GTATCAA 28555 0.0 40.054256 1 CAACGCA 34380 0.0 32.32406 5 ATCAACG 34755 0.0 32.17791 3 TATCAAC 36360 0.0 31.157722 2 TCAACGC 36445 0.0 30.737183 4 AACGCAG 37015 0.0 30.328337 6 GTACATG 37775 0.0 27.265087 1 ACGCAGA 41455 0.0 26.740435 7 TACATGG 38840 0.0 26.092808 2 CGCAGAG 42320 0.0 26.06005 8 GTGGTAT 8105 0.0 25.705023 1 ACATGGG 38680 0.0 25.268242 3 GCAGAGT 45745 0.0 23.618053 9 GTACTAG 3645 0.0 23.61135 1 TACCTGG 5815 0.0 23.534018 2 TGGTATC 8570 0.0 23.376612 2 CATGGGG 24345 0.0 22.447382 4 CTGTGCG 4325 0.0 21.0797 9 GAGTACT 26765 0.0 21.043112 12-13 >>END_MODULE