FastQCFastQC Report
Mon 6 Jun 2016
ERR1211324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211324_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9010957
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293360.3255592053097135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246190.2732118242268829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153400.17023719012309127No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157750.051.9707031
GTATCAA279300.040.1402661
CAACGCA343400.031.7622075
ATCAACG348200.031.3978213
TATCAAC364550.030.3341242
TCAACGC363400.030.1234424
AACGCAG370000.029.6692756
ACGCAGA418650.025.963777
CGCAGAG426700.025.4191138
GTACTAG34900.025.4139061
GTACATG376200.025.0982971
TACATGG385000.024.140862
GTGGTAT78400.024.0028781
ACATGGG381150.023.5869583
TACCTGG57400.023.213222
GCAGAGT465700.023.0680059
TGGTATC79800.022.7529142
GAGTACT271050.021.23344612-13
CATGGGG244650.021.192974
ACGGGCG25800.021.0924155
Produced by FastQC (version 0.11.5)