##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211324_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9010957 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.087940825819054 33.0 31.0 34.0 30.0 34.0 2 32.22385091838747 33.0 31.0 34.0 30.0 34.0 3 32.288166506620776 34.0 31.0 34.0 30.0 34.0 4 35.93323883356673 37.0 35.0 37.0 35.0 37.0 5 35.838991796320855 37.0 35.0 37.0 35.0 37.0 6 35.85456872116913 37.0 35.0 37.0 35.0 37.0 7 35.76987072516271 37.0 35.0 37.0 33.0 37.0 8 35.75099004467561 37.0 35.0 37.0 33.0 37.0 9 37.36198086396373 39.0 37.0 39.0 34.0 39.0 10-11 37.31161368320812 39.0 37.0 39.0 34.0 39.0 12-13 37.245133618992966 39.0 37.0 39.0 33.0 39.0 14-15 38.46910450244075 40.0 38.0 41.0 34.0 41.0 16-17 38.45414482612668 40.0 38.0 41.0 34.0 41.0 18-19 38.46758524094611 40.0 38.0 41.0 34.0 41.0 20-21 38.44049006115554 40.0 38.0 41.0 34.0 41.0 22-23 38.33193166941092 40.0 38.0 41.0 34.0 41.0 24-25 38.24520736254762 40.0 38.0 41.0 34.0 41.0 26-27 38.14613897280833 40.0 38.0 41.0 33.0 41.0 28-29 38.04605243372042 40.0 37.0 41.0 33.0 41.0 30-31 38.052631868069064 40.0 37.0 41.0 33.0 41.0 32-33 37.98837653980593 40.0 37.0 41.0 33.0 41.0 34-35 37.861543729484005 40.0 37.0 41.0 33.0 41.0 36-37 37.71785832514793 40.0 37.0 41.0 33.0 41.0 38-39 37.60446975831756 40.0 37.0 41.0 32.5 41.0 40-41 37.49866457025597 40.0 36.5 41.0 32.0 41.0 42-43 37.394594713968786 40.0 36.0 41.0 32.0 41.0 44-45 37.23415592816612 40.0 36.0 41.0 31.0 41.0 46-47 36.99120909133181 39.0 36.0 41.0 31.0 41.0 48-49 35.444022316386594 39.0 35.0 40.5 24.0 41.0 50-51 36.172277705908485 39.0 35.0 40.0 29.0 41.0 52-53 36.271227129371496 39.0 35.0 40.0 29.5 41.0 54-55 36.110671208396624 38.5 35.0 40.0 29.0 41.0 56-57 35.90052160941396 38.0 35.0 40.0 28.5 41.0 58-59 35.66512968600338 38.0 34.0 40.0 28.0 41.0 60-61 35.41549388150448 38.0 34.0 40.0 28.0 41.0 62-63 35.09895902288736 37.0 34.0 40.0 27.5 41.0 64-65 34.77226653062488 36.5 34.0 39.5 27.0 41.0 66-67 34.43933868511414 36.0 33.0 39.0 26.0 40.5 68-69 34.08125202461847 36.0 33.0 39.0 26.0 40.0 70-71 33.72667703330512 35.0 33.0 38.0 26.0 40.0 72-73 33.34886727347606 35.0 32.0 37.5 26.0 40.0 74-75 32.98114806229793 35.0 32.0 37.0 25.0 39.0 76-77 32.04119828781782 34.0 30.5 36.0 24.5 38.5 78-79 32.480214698616365 35.0 31.5 36.0 25.0 38.5 80-81 32.35034325432915 35.0 32.0 36.0 25.0 37.5 82-83 32.074306480432654 34.0 31.0 35.0 25.0 37.0 84-85 31.788070179449306 34.0 31.0 35.0 24.0 36.5 86-87 31.52757287599974 34.0 31.0 35.0 24.0 36.0 88-89 31.29407675566535 34.0 31.0 35.0 23.0 36.0 90-91 31.069684052426396 34.0 31.0 35.0 23.0 35.0 92-93 30.872028853317133 34.0 30.0 35.0 21.0 35.0 94-95 30.67408622635753 34.0 30.0 35.0 20.0 35.0 96-97 30.48508648970359 34.0 29.5 35.0 20.0 35.0 98-99 30.270456068095765 34.0 29.0 35.0 19.0 35.0 100 27.814448898158098 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.30800897745632483 1101 2 -0.2972242664156326 1101 3 -0.325970923946894 1101 4 -0.18068202594753302 1101 5 -0.13761843873206203 1101 6 -0.22408782692856732 1101 7 -0.2602988477784649 1101 8 -0.239733660114112 1101 9 -0.36607198896605553 1101 10-11 -0.3631244308233619 1101 12-13 -0.4045290207427996 1101 14-15 -0.49574080490901906 1101 16-17 -0.49921790283184464 1101 18-19 -0.5190830454751705 1101 20-21 -0.502794737420345 1101 22-23 -0.6576186049538748 1101 24-25 -0.5752143034805215 1101 26-27 -0.7004271284974948 1101 28-29 -0.6831876254596523 1101 30-31 -0.6422682456429101 1101 32-33 -0.5774439680921617 1101 34-35 -0.6408258491392402 1101 36-37 -0.6595010501737306 1101 38-39 -0.6125149079373031 1101 40-41 -0.6500788666215627 1101 42-43 -0.6605061389374569 1101 44-45 -0.5869329366947156 1101 46-47 -0.6387637189916049 1101 48-49 0.6093040439435526 1101 50-51 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1103 28-29 -0.711219413726532 1103 30-31 -0.7685391239149624 1103 32-33 -0.6842499764759538 1103 34-35 -0.7212646603453763 1103 36-37 -0.7274141368822171 1103 38-39 -0.7514034527317861 1103 40-41 -0.7082415566580238 1103 42-43 -0.7450177891481502 1103 44-45 -0.6715801601006177 1103 46-47 -0.709597891915287 1103 48-49 0.5503403709532577 1103 50-51 -0.3139996532302618 1103 52-53 -0.6347400734850908 1103 54-55 -0.7554107929466127 1103 56-57 -0.7590238180663462 1103 58-59 -0.9034244968349796 1103 60-61 -0.8719253485202358 1103 62-63 -0.9788139168027712 1103 64-65 -0.9513713764024772 1103 66-67 -0.8750704327717358 1103 68-69 -0.9087969000746909 1103 70-71 -0.9094619539417224 1103 72-73 -0.9917207765536347 1103 74-75 -1.0159186978107186 1103 76-77 -1.0101415726239686 1103 78-79 -0.719644556630648 1103 80-81 -0.7880722492658236 1103 82-83 -0.8015435706621687 1103 84-85 -0.7215283412563736 1103 86-87 -0.6753167762613295 1103 88-89 -0.7756639908523084 1103 90-91 -0.8862986752414344 1103 92-93 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70-71 -0.7531375131159308 1105 72-73 -0.6902864189861688 1105 74-75 -0.7910973557926084 1105 76-77 -0.7703393439656274 1105 78-79 -0.6841206882107898 1105 80-81 -0.6472079550487528 1105 82-83 -0.5909482775176933 1105 84-85 -0.5931675191205876 1105 86-87 -0.5811863644903958 1105 88-89 -0.6805239230475664 1105 90-91 -0.6961916462632516 1105 92-93 -0.7095039843356012 1105 94-95 -0.6887271997119555 1105 96-97 -0.7899468096790478 1105 98-99 -0.7175613076178493 1105 100 -0.7949181185465122 1106 1 -0.31198149294394284 1106 2 -0.29222899898961785 1106 3 -0.2986162608195748 1106 4 -0.1862036587923086 1106 5 -0.2685025760692241 1106 6 -0.35123056207167025 1106 7 -0.32327882151801646 1106 8 -0.32611303937594727 1106 9 -0.4277943019256014 1106 10-11 -0.4740051699589074 1106 12-13 -0.47370613109717397 1106 14-15 -0.6022806824788702 1106 16-17 -0.5613998477655571 1106 18-19 -0.6472442922178203 1106 20-21 -0.6308233623763186 1106 22-23 -0.6041634997638141 1106 24-25 -0.5901263149667955 1106 26-27 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0.3377223686334929 2311 6 0.3807339236083678 2311 7 0.4089739337129572 2311 8 0.22582486789377754 2311 9 0.47883676708945444 2311 10-11 0.3923324228435021 2311 12-13 0.49185274153838066 2311 14-15 0.5104500165718449 2311 16-17 0.49876087267101354 2311 18-19 0.6448269206289154 2311 20-21 0.7065615494887183 2311 22-23 0.7011371919985194 2311 24-25 0.6200019108256356 2311 26-27 0.6836844338441352 2311 28-29 0.7312763956095196 2311 30-31 0.613289229940662 2311 32-33 0.6403564200503595 2311 34-35 0.7738263203104694 2311 36-37 0.8125060906067461 2311 38-39 0.8420822950846443 2311 40-41 0.7493408731432964 2311 42-43 0.788280345903992 2311 44-45 0.8927989890905081 2311 46-47 0.9029755831485957 2311 48-49 -5.950045099120654 2311 50-51 -0.9526019585304653 2311 52-53 0.4633803489631205 2311 54-55 0.8163185588209458 2311 56-57 0.9993380851666558 2311 58-59 1.0064252672019052 2311 60-61 0.8293965931093865 2311 62-63 0.7397750919883279 2311 64-65 0.8973641162273154 2311 66-67 1.003471916091108 2311 68-69 0.9797566496623347 2311 70-71 0.9659937260819973 2311 72-73 0.5061493742036305 2311 74-75 0.7867750570518126 2311 76-77 0.9241599212605429 2311 78-79 0.7995640264908772 2311 80-81 0.7967516277300319 2311 82-83 0.8331392102664523 2311 84-85 0.845467964396402 2311 86-87 0.8998505364483584 2311 88-89 0.9519499653249568 2311 90-91 1.0412872502737862 2311 92-93 1.0292346134724717 2311 94-95 1.0522951128076627 2311 96-97 1.111061636597558 2311 98-99 1.0932367043812583 2311 100 1.3199088309806193 2312 1 0.10399852745234028 2312 2 0.24999372954577126 2312 3 0.23272699223510784 2312 4 0.04797923420711925 2312 5 0.23526833392457291 2312 6 0.15911441477322796 2312 7 0.335497786877184 2312 8 0.3674240554096784 2312 9 0.3157529957573644 2312 10-11 0.26048759145407274 2312 12-13 0.3223511031137818 2312 14-15 0.3893810412923031 2312 16-17 0.5380707550473005 2312 18-19 0.3918206395916286 2312 20-21 0.44353431885949135 2312 22-23 0.6462241033116101 2312 24-25 0.7451490134285592 2312 26-27 0.6960699507297647 2312 28-29 0.774119870298577 2312 30-31 0.7947366711656727 2312 32-33 0.801421558104451 2312 34-35 0.3902965391091229 2312 36-37 0.7479119954791997 2312 38-39 0.8290123987480982 2312 40-41 0.6376278615081148 2312 42-43 0.6406725710216321 2312 44-45 0.7210343016415806 2312 46-47 0.779849014771024 2312 48-49 -4.834269897397498 2312 50-51 -0.7986487637714532 2312 52-53 0.5227152524149616 2312 54-55 0.7784876134871226 2312 56-57 0.8498045738073614 2312 58-59 0.9312200839367577 2312 60-61 0.932063437914664 2312 62-63 0.6992556502508691 2312 64-65 0.917372163187089 2312 66-67 0.9562629638041997 2312 68-69 0.8637530846371817 2312 70-71 0.8585333064941381 2312 72-73 0.9542270901543191 2312 74-75 0.5957833623868112 2312 76-77 0.7841286504845293 2312 78-79 0.4397253297465866 2312 80-81 0.6364562965489 2312 82-83 0.7002494722792179 2312 84-85 0.729135477874312 2312 86-87 0.8651789272161601 2312 88-89 0.8768253131023158 2312 90-91 0.6896455345332306 2312 92-93 0.8878197026411101 2312 94-95 1.0279691695383804 2312 96-97 1.0232179952395803 2312 98-99 1.0402495633876434 2312 100 0.663129445944385 2313 1 0.11464944526285592 2313 2 0.2594202081452295 2313 3 0.3025615199769831 2313 4 0.07109261117166454 2313 5 0.1293655754695351 2313 6 0.2838779984380224 2313 7 0.18327045547182763 2313 8 0.2590744113859387 2313 9 0.49304058089732905 2313 10-11 0.27682538905477116 2313 12-13 0.051602594520147704 2313 14-15 0.1259848316167549 2313 16-17 0.11943422642796975 2313 18-19 0.433482353892245 2313 20-21 0.3644666992002996 2313 22-23 0.5226065560569211 2313 24-25 0.3580967765671801 2313 26-27 0.49541226304584285 2313 28-29 0.46094298636025854 2313 30-31 0.6415965330000191 2313 32-33 0.5667187807210681 2313 34-35 0.4285195472634271 2313 36-37 -0.3183009395155807 2313 38-39 0.1737185030686348 2313 40-41 0.3951765598890731 2313 42-43 0.5391716074206059 2313 44-45 0.4735110048092963 2313 46-47 0.39853814155981837 2313 48-49 -5.409189430301236 2313 50-51 -1.3527952581242317 2313 52-53 0.06567049307795259 2313 54-55 0.2720712318486207 2313 56-57 0.2958819464534983 2313 58-59 0.4110783569029266 2313 60-61 0.7366622770888398 2313 62-63 0.7503599316820839 2313 64-65 0.8893898834515355 2313 66-67 0.9217938992435677 2313 68-69 0.8963635360929345 2313 70-71 0.8901630437698884 2313 72-73 0.6973167205785344 2313 74-75 0.8691123482289811 2313 76-77 0.8921490976868505 2313 78-79 0.7556579915309456 2313 80-81 -0.14460253052827454 2313 82-83 0.2765928848383723 2313 84-85 0.6329785433761721 2313 86-87 0.767629021090837 2313 88-89 0.7086622845712753 2313 90-91 0.7274587857073591 2313 92-93 0.8122146281670517 2313 94-95 0.7707583397112145 2313 96-97 0.7603054097236068 2313 98-99 0.9683969190667057 2313 100 1.0245231829400794 2314 1 0.09129455387729024 2314 2 0.184805261159525 2314 3 0.20949724021860305 2314 4 0.08644342048594922 2314 5 0.21009891581516627 2314 6 0.2933563947985789 2314 7 0.13029152744987726 2314 8 0.28474002337894433 2314 9 0.4126223471046586 2314 10-11 0.30416163012935016 2314 12-13 0.2462409227731257 2314 14-15 0.29633331501216276 2314 16-17 0.46148432249845683 2314 18-19 0.5513869303248811 2314 20-21 0.5698863997126793 2314 22-23 0.29492725163481026 2314 24-25 0.4423061218296027 2314 26-27 0.48110154604879085 2314 28-29 0.6199388813958322 2314 30-31 0.5186642556453123 2314 32-33 0.5285050362750283 2314 34-35 0.4201985488871216 2314 36-37 0.5507692608398358 2314 38-39 0.5322715412899726 2314 40-41 0.29515178679938714 2314 42-43 0.32924109761473375 2314 44-45 0.5711176072516224 2314 46-47 0.7011504215635966 2314 48-49 -6.351209026807123 2314 50-51 -1.4963850028127084 2314 52-53 -0.9309644758193372 2314 54-55 -0.45124224858056294 2314 56-57 -0.12427379378874548 2314 58-59 0.40262312689132074 2314 60-61 0.6449359784839217 2314 62-63 0.573953697575817 2314 64-65 0.6543748470558768 2314 66-67 0.7663787149059189 2314 68-69 0.7528181827535221 2314 70-71 0.5155917669675603 2314 72-73 0.6605480654331899 2314 74-75 0.7035365684991319 2314 76-77 0.6772681775035103 2314 78-79 0.42103790320829404 2314 80-81 0.48404312952717987 2314 82-83 0.5517153546807734 2314 84-85 0.614999035002743 2314 86-87 0.6602967654823324 2314 88-89 0.5532485954443906 2314 90-91 0.7120598606927935 2314 92-93 0.8333618317313274 2314 94-95 0.8536019147809597 2314 96-97 0.8608992563860767 2314 98-99 0.9544873926476392 2314 100 -0.3877316903598178 2315 1 0.10570797329550885 2315 2 0.18135249121620234 2315 3 0.29193700922038346 2315 4 0.1365633013582439 2315 5 0.21863047877205588 2315 6 0.2664844193248328 2315 7 0.29962518764598656 2315 8 0.24937422304207502 2315 9 0.4039114086659339 2315 10-11 0.18929452745636155 2315 12-13 0.3790503772228391 2315 14-15 0.22044227407989325 2315 16-17 0.3225204198263327 2315 18-19 0.3805244877309306 2315 20-21 0.3304252582524043 2315 22-23 0.40095416599701394 2315 24-25 0.44368351355213065 2315 26-27 0.49556827871491294 2315 28-29 0.5364522123376148 2315 30-31 0.5223460839527121 2315 32-33 0.6069088259446787 2315 34-35 0.6230582817761459 2315 36-37 0.5077143770249108 2315 38-39 0.5949240288227244 2315 40-41 0.6142679942906213 2315 42-43 0.6543955107908275 2315 44-45 0.7423611275580555 2315 46-47 0.5891098374756538 2315 48-49 -4.69695502010979 2315 50-51 -0.8844185136024407 2315 52-53 0.24357678010760964 2315 54-55 0.1415880740964255 2315 56-57 0.561173018600563 2315 58-59 0.6537398317957823 2315 60-61 0.47534446049858303 2315 62-63 0.269299290766142 2315 64-65 0.43953615597872187 2315 66-67 -0.20350679983061326 2315 68-69 0.44633146841313476 2315 70-71 0.42729077260686665 2315 72-73 0.5385148467373568 2315 74-75 0.6780119855311852 2315 76-77 0.637820247917869 2315 78-79 0.5597607938733162 2315 80-81 0.5073715714888181 2315 82-83 0.4786744916922032 2315 84-85 0.42400078255451845 2315 86-87 0.4914928553270457 2315 88-89 0.4536984747843178 2315 90-91 0.5521430458289913 2315 92-93 0.6628531549891612 2315 94-95 0.2743524048670416 2315 96-97 0.3645177171035989 2315 98-99 0.37868519384672794 2315 100 0.4386032601745029 2316 1 0.19710744510320666 2316 2 0.2323548053055049 2316 3 0.14617676411899083 2316 4 -0.07904045002801752 2316 5 0.15200449929449888 2316 6 0.13332055722067793 2316 7 0.2034975593227415 2316 8 0.3069332912820144 2316 9 0.4320788037566956 2316 10-11 0.3671858012753759 2316 12-13 0.2781548702594421 2316 14-15 0.4414454225334268 2316 16-17 0.4201278296402222 2316 18-19 0.09401032173820312 2316 20-21 0.42941852066147845 2316 22-23 0.5497455424652244 2316 24-25 0.5906255938344316 2316 26-27 0.5858655114670768 2316 28-29 0.6365370390779006 2316 30-31 0.5515251738722355 2316 32-33 0.5404950695333142 2316 34-35 0.5146856424588933 2316 36-37 0.3706579159431911 2316 38-39 0.454373864986529 2316 40-41 0.04053472260338964 2316 42-43 0.016406399912220593 2316 44-45 0.2702578229220194 2316 46-47 0.29650587143196816 2316 48-49 -4.811410186779639 2316 50-51 -0.9428586284597031 2316 52-53 0.267238647435569 2316 54-55 0.4958786380792617 2316 56-57 0.39277082834847477 2316 58-59 0.3168567620838729 2316 60-61 0.43233019450192245 2316 62-63 0.33251194469198 2316 64-65 0.3431165956416393 2316 66-67 0.46990052999748144 2316 68-69 0.0941583488235338 2316 70-71 -0.2700778592453048 2316 72-73 -0.14747738425034385 2316 74-75 0.06282596040816202 2316 76-77 0.0857943449268852 2316 78-79 0.1644349059085215 2316 80-81 0.14554663085807817 2316 82-83 0.012596044861467703 2316 84-85 0.04930307035276016 2316 86-87 0.19110052362548657 2316 88-89 0.07094860973279538 2316 90-91 0.10428969470583738 2316 92-93 0.2341162347158665 2316 94-95 0.4395575715933049 2316 96-97 0.5149862142650505 2316 98-99 0.49902154781461405 2316 100 -0.22545216931226975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 5.0 10 6.0 11 19.0 12 42.0 13 188.0 14 691.0 15 2030.0 16 5086.0 17 10607.0 18 17957.0 19 27118.0 20 38996.0 21 55399.0 22 68534.0 23 72583.0 24 74301.0 25 80813.0 26 96904.0 27 118046.0 28 145755.0 29 179612.0 30 222137.0 31 275394.0 32 347727.0 33 451053.0 34 621910.0 35 873693.0 36 1345582.0 37 2004236.0 38 1652596.0 39 221900.0 40 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.939862579385625 20.309841890919376 14.294456918602979 23.455838611092027 2 15.28237720491358 20.543994839960654 40.537194099869666 23.636433855256104 3 18.630841517171717 25.92774059031061 30.0214150537496 25.420002838768074 4 12.556343756410268 17.08780923575844 38.454036548405455 31.901810459425832 5 13.076652038111558 38.049787396729414 34.83680625108549 14.036754314073544 6 30.662502549674542 37.61525139291578 17.815527634476883 13.906718422932798 7 27.49905587122694 32.462581926684116 22.162994302600403 17.875367899488545 8 25.884681174511986 35.84090060849316 19.93782003211988 18.33659818487498 9 26.231646787245072 16.11969539418724 20.772844076367434 36.87581374220025 10-11 24.886609147975534 26.614722355271436 27.919069505845606 20.579598990907424 12-13 25.310236537846688 24.153221631422316 28.370386597546293 22.166155233184703 14-15 22.760087105157364 25.739474123004268 26.010178174936655 25.490260596901713 16-17 21.858246026322277 28.633790193722685 26.975652436010435 22.532311343944606 18-19 21.54338921587053 27.75311982764978 29.224351315945498 21.479139640534196 20-21 22.470560256257166 26.94755868084024 29.16615432693652 21.41572673596607 22-23 22.497857741376073 26.699653260120055 28.877945886516642 21.924543111987234 24-25 22.122399262319977 26.86089917459341 29.16148824658169 21.855213316504923 26-27 21.99098685338175 27.08360834600264 28.916817136398016 22.00858766421759 28-29 21.77157439554848 27.14057101129693 29.185171662400517 21.902682930754075 30-31 22.311365633232228 26.954912154983756 29.05249637125821 21.6812258405258 32-33 22.211902285310167 27.182297625278746 28.74233024656418 21.863469842846914 34-35 22.322073740524477 27.138426149255668 28.942169095684378 21.597331014535477 36-37 22.298997247865078 26.927965193940373 28.78668856475398 21.986348993440565 38-39 22.182988646806468 27.100267543359664 28.977794112048088 21.738949697785785 40-41 22.119242936237914 26.72198067675742 29.267351098097155 21.891425288907516 42-43 22.007789732599942 26.824596617005998 28.854167724466162 22.3134459259279 44-45 21.937680793406386 27.215689839132402 28.814410343312623 22.032219024148592 46-47 22.011531265356453 26.998338763037466 28.503080377841922 22.487049593764162 48-49 22.380647585646095 27.110213878452715 28.328514976051988 22.180623559849195 50-51 22.2007598047282 27.13057196470065 28.20032902709243 22.46833920347872 52-53 22.017289111839894 27.393089372327612 28.129798538258548 22.45982297757395 54-55 22.02070612406893 27.30218062243121 28.105679988919086 22.57143326458077 56-57 22.265191076896336 27.184969867172725 27.86842307663708 22.681415979293856 58-59 21.926194461360474 27.234189597090676 28.274489408715088 22.565126532833766 60-61 22.15937448011978 27.505227518728454 27.93373367416574 22.401664326986026 62-63 22.089532029750416 27.29706408058782 28.004171894482923 22.60923199517885 64-65 21.816359543283358 27.136066241483654 28.032631243209387 23.0149429720236 66-67 21.974486569052136 27.12729823883673 27.85743960423572 23.040775587875416 68-69 21.744594923793294 27.16892824171502 28.012400258626418 23.074076575865277 70-71 21.875724479680326 26.8666939627438 28.085909490803836 23.171672066772043 72-73 21.976974662227132 27.141949548454935 27.842628760010307 23.038447029307623 74-75 21.973093498511115 27.279888436186546 27.72800271695399 23.01901534834835 76-77 21.795707437107037 27.10993570506743 27.787792037484245 23.306564820341293 78-79 21.720818317926703 27.039857095133403 27.778948057699115 23.460376529240783 80-81 21.664286818921063 27.37543310167805 27.655177346609133 23.305102732791752 82-83 21.993582804130458 26.985209327481936 27.963033391183956 23.05817447720365 84-85 21.969534040531183 27.08198384886669 27.549691045180445 23.398791065421676 86-87 21.488870330636896 27.319545774356506 27.86666208599624 23.324921809010352 88-89 21.69060396593837 26.8421942183589 27.861217967308804 23.605983848393926 90-91 21.83330777594501 26.799055603164636 27.828743631534497 23.538892989355862 92-93 21.843248539294414 26.903032235275642 27.68127053152563 23.572448693904313 94-95 21.366362492781228 26.69433407305578 27.943784497436646 23.995518936726345 96-97 21.47168519136205 26.66371422773514 27.87308589261161 23.9915146882912 98-99 21.24334802700913 26.73133326932714 27.813735321817028 24.211583381846697 100 21.35303887662106 26.56050848641165 27.713026767052785 24.373425869914506 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2569.0 1 1805.0 2 1605.0 3 2064.5 4 1569.5 5 1344.0 6 2131.0 7 4067.5 8 6459.0 9 7773.5 10 7096.5 11 5919.5 12 5957.5 13 6708.0 14 7600.0 15 8550.0 16 9452.5 17 10430.5 18 11477.0 19 12481.5 20 13716.5 21 15085.5 22 17041.0 23 20143.0 24 23818.0 25 28422.0 26 35083.0 27 43391.0 28 52274.0 29 65908.5 30 86235.5 31 106721.0 32 132194.0 33 162999.5 34 192167.0 35 219770.0 36 252027.0 37 286156.0 38 301742.5 39 303490.5 40 297685.0 41 289981.5 42 290791.0 43 300960.5 44 315285.0 45 335120.5 46 350530.5 47 355707.0 48 361794.5 49 363815.5 50 356234.5 51 344925.0 52 332298.0 53 319575.5 54 305914.5 55 279861.5 56 251023.0 57 218489.0 58 185037.0 59 158871.5 60 129487.0 61 100611.5 62 78757.0 63 60986.5 64 44506.0 65 30889.5 66 21557.0 67 15460.0 68 11423.0 69 8379.5 70 6179.0 71 4487.5 72 3304.0 73 2359.0 74 1652.5 75 1130.0 76 710.5 77 402.0 78 230.5 79 143.5 80 93.0 81 52.5 82 26.0 83 17.0 84 14.5 85 13.5 86 9.5 87 3.5 88 2.5 89 2.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028365466620249104 2 0.001919884869054419 3 6.658560239495095E-5 4 0.021895565587539703 5 4.6609921676465666E-4 6 3.3292801197475476E-5 7 4.4390401596633966E-5 8 0.0037509889349155705 9 0.009832473953654422 10-11 9.876864355251058E-4 12-13 1.109760039915849E-4 14-15 1.0542720379200566E-4 16-17 3.884160139705472E-4 18-19 2.7744000997896224E-5 20-21 9.987840359242643E-5 22-23 2.607936093802245E-4 24-25 4.4390401596633966E-5 26-27 9.432960339284717E-5 28-29 0.001131955240714166 30-31 0.0028631809029828906 32-33 0.009865766754851898 34-35 0.01876604227497701 36-37 0.021651418378758218 38-39 0.021640320778359055 40-41 0.005115993784012065 42-43 0.005620934602173775 44-45 0.009383021137488505 46-47 0.012257299640870554 48-49 1.1988349295196947 50-51 0.018516346265995944 52-53 0.018899213479766912 54-55 0.009643814746868729 56-57 0.0075907586730244074 58-59 0.008500761905755405 60-61 0.0051936769868061745 62-63 0.0073466114642429204 64-65 0.019293178293937036 66-67 0.028459796223641948 68-69 0.028814919436415024 70-71 0.025929543332633812 72-73 0.022811117620470278 74-75 0.00962161954607041 76-77 0.014476819720702253 78-79 0.008511859506154563 80-81 0.0025136064904093982 82-83 0.013966330102340961 84-85 0.023476973644419787 86-87 0.02315514323284419 88-89 0.03190005234738109 90-91 0.020203181526668033 92-93 0.013145107672803233 94-95 0.006608621037698881 96-97 0.003634464130724406 98-99 0.01566981176361179 100 0.035556711678903806 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9010957.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.23114911914544 #Duplication Level Percentage of deduplicated Percentage of total 1 72.26613710465416 39.19075657587109 2 15.730671254168655 17.061847570581502 3 5.4704742936896125 8.900103215205995 4 2.4536469311058173 5.322563704261326 5 1.2709780608602763 3.446330037283798 6 0.7342923767739045 2.3892911629126408 7 0.47561800639811536 1.8055317720108808 8 0.3068282544868875 1.33117190584364 9 0.21598792711380235 1.0541946134919358 >10 0.9747190671637336 9.047607324114708 >50 0.055099613349883315 2.056738485418672 >100 0.03970477219153334 4.273289734931347 >500 0.004107919916655534 1.4759181242300154 >1k 0.0016127045742057602 1.396123331121166 >5k 3.042838817532004E-5 0.10504959662658452 >10k+ 9.128516452596012E-5 1.1434828460947493 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29336 0.3255592053097135 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24619 0.2732118242268829 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15340 0.17023719012309127 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2195200798316983E-5 2 0.0 0.0 0.0 0.0 2.2195200798316983E-5 3 0.0 0.0 0.0 0.0 2.2195200798316983E-5 4 0.0 0.0 0.0 0.0 2.2195200798316983E-5 5 0.0 0.0 0.0 0.0 2.2195200798316983E-5 6 0.0 0.0 0.0 0.0 2.2195200798316983E-5 7 0.0 0.0 0.0 0.0 2.2195200798316983E-5 8 0.0 0.0 0.0 0.0 4.4390401596633966E-5 9 0.0 0.0 0.0 0.0 4.4390401596633966E-5 10-11 0.0 0.0 0.0 0.0 4.4390401596633966E-5 12-13 0.0 0.0 0.0 0.0 4.4390401596633966E-5 14-15 0.0 0.0 0.0 0.0 7.768320279410943E-5 16-17 0.0 0.0 0.0 0.0 1.2762240459032265E-4 18-19 0.0 0.0 0.0 0.0 1.442688051890604E-4 20-21 0.0 0.0 0.0 6.10368021953717E-5 1.6091520578779812E-4 22-23 0.0 0.0 0.0 4.3280641556718113E-4 2.0530560738443208E-4 24-25 0.0 0.0 0.0 0.0011208576403150075 2.3859840858190753E-4 26-27 0.0 0.0 0.0 0.00228055688202707 2.552448091806453E-4 28-29 0.0 0.0 0.0 0.005715264205566623 2.552448091806453E-4 30-31 5.548800199579246E-6 0.0 0.0 0.01861067586938879 2.552448091806453E-4 32-33 1.1097600399158491E-5 0.0 0.0 0.0478029137193752 2.663424095798038E-4 34-35 1.1097600399158491E-5 0.0 0.0 0.09328642895532628 2.7744000997896226E-4 36-37 1.1097600399158491E-5 0.0 0.0 0.16625870037999294 2.9408641057770003E-4 38-39 1.1097600399158491E-5 0.0 0.0 0.2678572320342889 3.1073281117643774E-4 40-41 1.1097600399158491E-5 0.0 0.0 0.3758590791188994 3.1073281117643774E-4 42-43 1.6646400598737738E-5 0.0 0.0 0.480059998066798 3.1073281117643774E-4 44-45 2.2195200798316983E-5 0.0 0.0 0.6097354587309649 3.3292801197475476E-4 46-47 2.2195200798316983E-5 0.0 0.0 0.7676043732091941 3.884160139705472E-4 48-49 2.2195200798316983E-5 0.0 0.0 0.9349395408279054 3.884160139705472E-4 50-51 2.2195200798316983E-5 0.0 0.0 1.1107976655531704 3.884160139705472E-4 52-53 3.3292801197475476E-5 0.0 0.0 1.2823277261227637 4.383552157667604E-4 54-55 3.3292801197475476E-5 0.0 0.0 1.4758088402818923 4.882944175629736E-4 56-57 3.3292801197475476E-5 0.0 0.0 1.700574089966249 4.882944175629736E-4 58-59 3.3292801197475476E-5 0.0 0.0 1.9510191869742581 4.993920179621321E-4 60-61 3.3292801197475476E-5 0.0 0.0 2.206202959352708 5.215872187604491E-4 62-63 3.3292801197475476E-5 0.0 0.0 2.461519902935948 5.326848191596076E-4 64-65 3.3292801197475476E-5 0.0 0.0 2.7289443285546695 5.326848191596076E-4 66-67 3.3292801197475476E-5 0.0 0.0 3.0252225152112033 5.326848191596076E-4 68-69 3.3292801197475476E-5 0.0 0.0 3.3536781942250973 5.326848191596076E-4 70-71 3.3292801197475476E-5 0.0 0.0 3.7058716404927914 5.326848191596076E-4 72-73 3.3292801197475476E-5 0.0 0.0 4.050618596892649 5.881728211554001E-4 74-75 3.3292801197475476E-5 0.0 0.0 4.390676817123864 5.881728211554001E-4 76-77 3.3292801197475476E-5 0.0 0.0 4.766164126629391 5.881728211554001E-4 78-79 3.3292801197475476E-5 0.0 0.0 5.174039782899863 5.881728211554001E-4 80-81 3.3292801197475476E-5 0.0 0.0 5.603322710340311 6.103680219537171E-4 82-83 3.3292801197475476E-5 0.0 0.0 6.035169183472965 6.769536243486679E-4 84-85 3.3292801197475476E-5 0.0 0.0 6.4651901013399575 6.769536243486679E-4 86-87 3.3292801197475476E-5 0.0 0.0 6.907773502858797 6.769536243486679E-4 88 3.3292801197475476E-5 0.0 0.0 7.2605384755470475 6.769536243486679E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15775 0.0 51.970703 1 GTATCAA 27930 0.0 40.140266 1 CAACGCA 34340 0.0 31.762207 5 ATCAACG 34820 0.0 31.397821 3 TATCAAC 36455 0.0 30.334124 2 TCAACGC 36340 0.0 30.123442 4 AACGCAG 37000 0.0 29.669275 6 ACGCAGA 41865 0.0 25.96377 7 CGCAGAG 42670 0.0 25.419113 8 GTACTAG 3490 0.0 25.413906 1 GTACATG 37620 0.0 25.098297 1 TACATGG 38500 0.0 24.14086 2 GTGGTAT 7840 0.0 24.002878 1 ACATGGG 38115 0.0 23.586958 3 TACCTGG 5740 0.0 23.21322 2 GCAGAGT 46570 0.0 23.068005 9 TGGTATC 7980 0.0 22.752914 2 GAGTACT 27105 0.0 21.233446 12-13 CATGGGG 24465 0.0 21.19297 4 ACGGGCG 2580 0.0 21.092415 5 >>END_MODULE