FastQCFastQC Report
Mon 6 Jun 2016
ERR1211323_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211323_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6039683
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241110.3992096936213374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187210.3099665992403906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117850.19512613493125386No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97450.053.2863121
GTATCAA180600.042.50411
TACCTGG58750.036.4259032
ATCAACG227000.033.2417453
CAACGCA227600.032.988965
TATCAAC232650.032.5281832
TCAACGC232950.032.4733664
GTACCTG69150.031.9693721
AACGCAG243000.031.4012876
ACCTGGG69650.030.2893093
ACGCAGA275350.027.3375477
GTACATG226250.027.233981
GTGGTAT45400.026.8331931
CGCAGAG284050.026.4340728
TACATGG231100.026.0912512
ACATGGG235600.024.8688283
TGGTATC48650.023.7825872
GCAGAGT309050.023.5530939
CATGGGG165900.022.9971374
ATGGGGG104750.021.8443475
Produced by FastQC (version 0.11.5)