##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211323_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6039683 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.283008893016405 31.0 31.0 34.0 28.0 34.0 2 31.294320082693083 31.0 31.0 34.0 28.0 34.0 3 31.340044833478842 31.0 31.0 34.0 28.0 34.0 4 34.95779960637007 37.0 35.0 37.0 32.0 37.0 5 35.00434079073355 37.0 35.0 37.0 32.0 37.0 6 35.06981839940937 37.0 35.0 37.0 32.0 37.0 7 35.0011803599626 37.0 35.0 37.0 32.0 37.0 8 34.999068659729325 37.0 35.0 37.0 32.0 37.0 9 36.54227945407069 39.0 37.0 39.0 32.0 39.0 10-11 36.462630406264694 39.0 37.0 39.0 32.0 39.0 12-13 36.37633514871558 39.0 37.0 39.0 32.0 39.0 14-15 37.487015874839784 40.0 37.0 41.0 32.0 41.0 16-17 37.47980324464049 40.0 37.0 41.0 32.0 41.0 18-19 37.48396381068344 40.0 37.0 41.0 31.0 41.0 20-21 37.44742282003874 40.0 37.0 41.0 31.0 41.0 22-23 37.32002788888092 40.0 37.0 41.0 31.0 41.0 24-25 37.207624059077276 40.0 36.0 41.0 31.0 41.0 26-27 37.06113284422378 40.0 36.0 41.0 30.0 41.0 28-29 36.91031275979219 39.0 36.0 41.0 30.0 41.0 30-31 36.71034431773985 39.0 36.0 41.0 30.0 41.0 32-33 36.57343423487623 39.0 35.0 41.0 30.0 41.0 34-35 36.41467283961757 39.0 35.0 41.0 29.5 41.0 36-37 36.30625671910264 39.0 35.0 40.5 29.0 41.0 38-39 36.14784658400118 39.0 35.0 40.0 28.0 41.0 40-41 35.97734997018883 39.0 35.0 40.0 27.5 41.0 42-43 35.818582862709846 38.0 35.0 40.0 27.0 41.0 44-45 35.68167650520731 38.0 34.5 40.0 26.5 41.0 46-47 35.50309387098628 38.0 34.0 40.0 26.0 41.0 48-49 35.25720654544287 38.0 34.0 40.0 25.0 41.0 50-51 34.59108880383292 37.5 33.5 39.5 24.0 40.5 52-53 34.83880221859326 38.0 33.5 40.0 24.5 40.5 54-55 35.20033907408717 38.0 34.0 40.0 26.0 41.0 56-57 35.103065508570566 38.0 34.0 40.0 25.0 41.0 58-59 34.88630032404019 38.0 34.0 40.0 25.0 41.0 60-61 34.61381474822437 37.5 33.0 40.0 24.5 41.0 62-63 34.33924661277753 37.0 33.0 40.0 24.0 41.0 64-65 34.062345242291684 36.5 33.0 40.0 23.0 41.0 66-67 33.83570338045888 36.0 32.5 39.0 23.0 41.0 68-69 33.48954042786683 35.5 32.0 39.0 22.5 41.0 70-71 33.13782428647331 35.0 32.0 38.5 22.0 40.0 72-73 32.76664578256839 35.0 31.5 37.5 22.0 40.0 74-75 32.39087953788303 35.0 31.0 37.0 21.0 39.5 76-77 32.03026888994009 35.0 31.0 36.5 20.0 39.0 78-79 31.661459384540546 35.0 30.5 36.0 20.0 39.0 80-81 31.327426207633742 34.5 30.0 36.0 19.5 37.5 82-83 31.006536104626683 34.0 30.0 35.0 18.5 37.0 84-85 30.699343326462664 34.0 29.5 35.0 18.0 37.0 86-87 30.43347010099702 34.0 29.0 35.0 18.0 36.0 88-89 30.162219027058207 34.0 29.0 35.0 17.5 36.0 90-91 29.895283576969188 34.0 29.0 35.0 16.5 35.5 92-93 29.64903257339831 34.0 29.0 35.0 15.0 35.0 94-95 29.415971583276804 34.0 27.5 35.0 13.5 35.0 96-97 29.200891172599622 34.0 27.0 35.0 11.0 35.0 98-99 28.951532969528365 33.5 26.5 35.0 10.0 35.0 100 27.25712028263735 31.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3237060961630327 1101 2 -0.3336864365877332 1101 3 -0.4330513268922438 1101 4 -0.34784337903511187 1101 5 -0.27187976482644416 1101 6 -0.2658382056212787 1101 7 -0.4244133488360049 1101 8 -0.5311453192894646 1101 9 -0.5359306231404872 1101 10-11 -0.5434436090045907 1101 12-13 -0.5102392890989762 1101 14-15 -0.6023273617045177 1101 16-17 -0.6652585373998932 1101 18-19 -0.6944654871501399 1101 20-21 -0.7186378273594869 1101 22-23 -0.7387230759740078 1101 24-25 -0.705269395880336 1101 26-27 -0.6844637921284047 1101 28-29 -0.8502172256973495 1101 30-31 -0.8868078156287069 1101 32-33 -0.9512092632798357 1101 34-35 -0.8728529816603867 1101 36-37 -0.9308218179169216 1101 38-39 -1.0352935888832064 1101 40-41 -1.0053867888532366 1101 42-43 -1.1214846881278007 1101 44-45 -1.14010050438889 1101 46-47 -0.9393485294799859 1101 48-49 -0.9134845371687064 1101 50-51 -0.8462049980613315 1101 52-53 -0.7712676487985988 1101 54-55 -0.6693557812121824 1101 56-57 -0.7055269432850437 1101 58-59 -0.8447274403600318 1101 60-61 -0.886295272203462 1101 62-63 -0.8491088397390385 1101 64-65 -0.9227524971764396 1101 66-67 -0.8433728352668268 1101 68-69 -0.7765112725037753 1101 70-71 -0.7725476704874623 1101 72-73 -0.9111870257356784 1101 74-75 -0.9427616675503572 1101 76-77 -1.1586844681120674 1101 78-79 -1.0869883712093191 1101 80-81 -1.0372779610579812 1101 82-83 -1.1204699760502486 1101 84-85 -0.9888007744608238 1101 86-87 -1.0298549691160446 1101 88-89 -1.1190285338112247 1101 90-91 -1.214004198822412 1101 92-93 -1.19462388588774 1101 94-95 -1.1508164973171695 1101 96-97 -1.3480829647138606 1101 98-99 -1.3993731012076935 1101 100 -1.3551137928527197 1102 1 -0.378815897960159 1102 2 -0.440729802480444 1102 3 -0.34651277863409646 1102 4 -0.22243289620809747 1102 5 -0.32225574054049133 1102 6 -0.31652058833133623 1102 7 -0.3579107383472717 1102 8 -0.35594772468960656 1102 9 -0.5010435828703947 1102 10-11 -0.4613702420824026 1102 12-13 -0.4641410530051857 1102 14-15 -0.473847815148126 1102 16-17 -0.49634562653209 1102 18-19 -0.560596845128714 1102 20-21 -0.6367550253465097 1102 22-23 -0.7566291460793195 1102 24-25 -0.7445486650303437 1102 26-27 -0.69003319773892 1102 28-29 -0.7591690532265147 1102 30-31 -0.6744670659299814 1102 32-33 -0.6996408021764893 1102 34-35 -0.7953500660673782 1102 36-37 -0.7323383271178585 1102 38-39 -0.8868763932945285 1102 40-41 -0.8743729795278412 1102 42-43 -0.87306155012066 1102 44-45 -0.8226169105077261 1102 46-47 -0.8608222419108955 1102 48-49 -0.8951298963068979 1102 50-51 -0.8308688819792778 1102 52-53 -0.6921758627841825 1102 54-55 -0.5369037162200954 1102 56-57 -0.6927786519057193 1102 58-59 -0.7329284361343298 1102 60-61 -0.7404141431533091 1102 62-63 -0.7458581497804957 1102 64-65 -0.7901174831908762 1102 66-67 -0.6826267247116462 1102 68-69 -0.6577971336964907 1102 70-71 -0.7306714074730039 1102 72-73 -0.743334297103381 1102 74-75 -0.8231930263372824 1102 76-77 -0.7787150413373247 1102 78-79 -0.7283253037977069 1102 80-81 -0.7886718740573642 1102 82-83 -0.9458292117810814 1102 84-85 -0.9019145162250304 1102 86-87 -0.7903841229417097 1102 88-89 -0.9585121867491324 1102 90-91 -0.9452057805545842 1102 92-93 -1.0057569751510194 1102 94-95 -1.0629621325991145 1102 96-97 -1.0605766673870392 1102 98-99 -1.0866437427276772 1102 100 -1.1316692718260128 1103 1 -0.5283668037883018 1103 2 -0.574414556643525 1103 3 -0.4762917828128508 1103 4 -0.40987121433597906 1103 5 -0.4665914939157858 1103 6 -0.558849184200966 1103 7 -0.5159487409730517 1103 8 -0.4977894877267204 1103 9 -0.6509228339525492 1103 10-11 -0.7357383114566929 1103 12-13 -0.8092027499201606 1103 14-15 -0.9299286452927831 1103 16-17 -0.9050272743044303 1103 18-19 -0.9916459036120173 1103 20-21 -1.0790436121529439 1103 22-23 -0.9530276300356917 1103 24-25 -1.0751599155933818 1103 26-27 -0.961358598900631 1103 28-29 -1.0330977725823658 1103 30-31 -1.0826310750748505 1103 32-33 -1.1649668068279695 1103 34-35 -1.116086923321923 1103 36-37 -1.1776673626991894 1103 38-39 -1.1362411491305693 1103 40-41 -1.1448496019606225 1103 42-43 -1.1995093000037755 1103 44-45 -1.225036538175523 1103 46-47 -1.1524052892080334 1103 48-49 -1.0609469786709553 1103 50-51 -1.05267757638142 1103 52-53 -0.9858732614947243 1103 54-55 -0.9612849263094674 1103 56-57 -0.9653606164789537 1103 58-59 -0.8935064947603024 1103 60-61 -1.0588066494899664 1103 62-63 -1.0301729661315662 1103 64-65 -0.9388696544996407 1103 66-67 -0.8946039678142839 1103 68-69 -1.008215764031199 1103 70-71 -1.0655651698963098 1103 72-73 -1.1054625819821169 1103 74-75 -0.9779034835943818 1103 76-77 -0.9903727676158205 1103 78-79 -1.0368162246042623 1103 80-81 -1.0074459888642409 1103 82-83 -1.1786465472267942 1103 84-85 -1.1293058033177132 1103 86-87 -1.2531042775960728 1103 88-89 -1.3290864184069378 1103 90-91 -1.1978593977826009 1103 92-93 -1.281171007961671 1103 94-95 -1.479619600929631 1103 96-97 -1.503787290806038 1103 98-99 -1.508672398199895 1103 100 -1.5011153550922778 1104 1 -0.3769711642620912 1104 2 -0.4685104840671066 1104 3 -0.46226083323410094 1104 4 -0.32454935016178155 1104 5 -0.27435466388772767 1104 6 -0.29055407170676517 1104 7 -0.33704485735017187 1104 8 -0.33906486378746337 1104 9 -0.5015402750977671 1104 10-11 -0.4511944337225131 1104 12-13 -0.5255996293011194 1104 14-15 -0.7311701629662934 1104 16-17 -0.6714298190309123 1104 18-19 -0.6920622861283192 1104 20-21 -0.6576586365188177 1104 22-23 -0.6427281460141643 1104 24-25 -0.7685313635236355 1104 26-27 -0.8500368746074827 1104 28-29 -0.741700114725873 1104 30-31 -0.7725903709938606 1104 32-33 -0.7621229338053936 1104 34-35 -0.7809001715245429 1104 36-37 -0.9261710349027794 1104 38-39 -0.8347655507926461 1104 40-41 -0.8975026710372376 1104 42-43 -0.9564138562947306 1104 44-45 -0.970654584982853 1104 46-47 -1.0468143838890782 1104 48-49 -0.8753754806248111 1104 50-51 -0.9423356226474837 1104 52-53 -0.8282820490243736 1104 54-55 -0.7604743335643391 1104 56-57 -0.8283025236184756 1104 58-59 -0.8170603113853758 1104 60-61 -0.7789980713253968 1104 62-63 -0.8274152389671059 1104 64-65 -0.8994747655705666 1104 66-67 -0.8693724589721867 1104 68-69 -0.7820653116920724 1104 70-71 -0.843621925409181 1104 72-73 -0.8602695094524861 1104 74-75 -0.7858716168002644 1104 76-77 -0.766886266707651 1104 78-79 -0.7969126121028047 1104 80-81 -0.8694155596821176 1104 82-83 -0.8618489037496779 1104 84-85 -0.8816536773784662 1104 86-87 -0.8511898146944681 1104 88-89 -0.9216366928460573 1104 90-91 -0.9355962397934761 1104 92-93 -0.9651021643133042 1104 94-95 -1.0799188126885078 1104 96-97 -1.065716674352867 1104 98-99 -1.1209503023905043 1104 100 -1.1291751348399472 1105 1 -0.3989258781176801 1105 2 -0.46429127111413493 1105 3 -0.3799207026618774 1105 4 -0.2694485428397613 1105 5 -0.27134299617372903 1105 6 -0.30246387037993827 1105 7 -0.2596776961208178 1105 8 -0.2763836400606081 1105 9 -0.4292377044271092 1105 10-11 -0.4538285037032139 1105 12-13 -0.4837244963864009 1105 14-15 -0.5755834274158218 1105 16-17 -0.6629411853382976 1105 18-19 -0.6444129379501078 1105 20-21 -0.6337221541658948 1105 22-23 -0.6467143884410262 1105 24-25 -0.5953966867322649 1105 26-27 -0.6847114274144062 1105 28-29 -0.7657805846963441 1105 30-31 -0.7537618113977018 1105 32-33 -0.6637937817893302 1105 34-35 -0.7772880890366665 1105 36-37 -0.7241275460052634 1105 38-39 -0.7007417004950014 1105 40-41 -0.785540106218825 1105 42-43 -0.7229916999502422 1105 44-45 -0.8399870612660649 1105 46-47 -0.7628833540378253 1105 48-49 -0.7464044684262348 1105 50-51 -0.8930709908352483 1105 52-53 -0.6633446728449357 1105 54-55 -0.6004509245835408 1105 56-57 -0.6874387418208769 1105 58-59 -0.7047287033450971 1105 60-61 -0.7419913348475191 1105 62-63 -0.6874472024593317 1105 64-65 -0.7317350273865912 1105 66-67 -0.7177331107779921 1105 68-69 -0.721979862516271 1105 70-71 -0.6779505401699524 1105 72-73 -0.7383957836349708 1105 74-75 -0.721650421119417 1105 76-77 -0.6525768076565797 1105 78-79 -0.606760853471144 1105 80-81 -0.7739034002835901 1105 82-83 -0.7410388155022964 1105 84-85 -0.7359247770409247 1105 86-87 -0.7798213646919869 1105 88-89 -0.7587401672568745 1105 90-91 -0.8393825305093507 1105 92-93 -0.8056459114451471 1105 94-95 -0.7405414372126131 1105 96-97 -1.020619128938005 1105 98-99 -1.0357612796674864 1105 100 -0.9784839782859898 1106 1 -0.48326187775918683 1106 2 -0.5540085400207566 1106 3 -0.5609662283535002 1106 4 -0.36792749885047726 1106 5 -0.38225277381067 1106 6 -0.41456596469618745 1106 7 -0.42939701357153126 1106 8 -0.4125465249228242 1106 9 -0.5823081199294649 1106 10-11 -0.5858912595711772 1106 12-13 -0.565248549174818 1106 14-15 -0.7484096500605588 1106 16-17 -0.7756487204596141 1106 18-19 -0.6923999232934221 1106 20-21 -0.6877635475599817 1106 22-23 -0.7105953552258057 1106 24-25 -0.8032200446161184 1106 26-27 -0.6629746141167843 1106 28-29 -0.7427010147625666 1106 30-31 -0.7801470593195532 1106 32-33 -0.8457393865419576 1106 34-35 -0.8492625654576003 1106 36-37 -0.8788880103845997 1106 38-39 -0.977288911434222 1106 40-41 -1.0340627538340357 1106 42-43 -1.0046985212819877 1106 44-45 -0.9154522248135066 1106 46-47 -0.9465561375291287 1106 48-49 -1.0175584069123005 1106 50-51 -0.9781990408453325 1106 52-53 -0.8658922294759677 1106 54-55 -0.875007046689575 1106 56-57 -0.8012414478195922 1106 58-59 -0.7428194602501321 1106 60-61 -0.7896404704579396 1106 62-63 -0.8895229537788794 1106 64-65 -0.9109090159979658 1106 66-67 -0.956851500622335 1106 68-69 -0.9147840313034976 1106 70-71 -0.9286190377229033 1106 72-73 -0.8389916218896118 1106 74-75 -0.7832027694231272 1106 76-77 -0.8809494634339536 1106 78-79 -0.8669709252662123 1106 80-81 -0.9952837989174661 1106 82-83 -0.8735724164267005 1106 84-85 -0.8606524647147715 1106 86-87 -0.8595274946401013 1106 88-89 -0.9602300029572355 1106 90-91 -0.9860589277604639 1106 92-93 -1.084328889644155 1106 94-95 -1.1476447638561673 1106 96-97 -1.071200563366986 1106 98-99 -1.0944150981216652 1106 100 -1.0409450479460922 1107 1 -0.5810567269151967 1107 2 -0.5556126291021606 1107 3 -0.6310845038256794 1107 4 -0.48125430464552466 1107 5 -0.3902494484135133 1107 6 -0.3594895774056468 1107 7 -0.550900787343906 1107 8 -0.6001814305250619 1107 9 -0.6823122075016101 1107 10-11 -0.6451953284033749 1107 12-13 -0.6185259645212042 1107 14-15 -0.7407744426068419 1107 16-17 -0.70511797751508 1107 18-19 -0.7437040789079177 1107 20-21 -0.8546731417835502 1107 22-23 -0.8723463390729762 1107 24-25 -0.9035590615613032 1107 26-27 -0.889130956833931 1107 28-29 -0.784307719890144 1107 30-31 -0.8201891361583549 1107 32-33 -0.8421558434847256 1107 34-35 -0.9328601446087248 1107 36-37 -1.0475526194303342 1107 38-39 -1.0491607239140208 1107 40-41 -1.0146670353457594 1107 42-43 -0.9731510167762281 1107 44-45 -0.9526679656136352 1107 46-47 -0.8989174650719463 1107 48-49 -0.9018489238706877 1107 50-51 -0.8905646383288968 1107 52-53 -0.7790045635048202 1107 54-55 -0.6599774841101009 1107 56-57 -0.689161068543207 1107 58-59 -0.6598268417765638 1107 60-61 -0.7103777615672797 1107 62-63 -0.728060709739502 1107 64-65 -0.6837021043536637 1107 66-67 -0.6396099326548139 1107 68-69 -0.5673218161915585 1107 70-71 -0.6183841972702737 1107 72-73 -0.6929957132349003 1107 74-75 -0.5417716412406364 1107 76-77 -0.5646176946974641 1107 78-79 -0.6324124207893789 1107 80-81 -0.6121481468616707 1107 82-83 -0.7301332212633689 1107 84-85 -0.6159810237638368 1107 86-87 -0.7773076788449664 1107 88-89 -0.7685241270408341 1107 90-91 -0.676459075919233 1107 92-93 -0.6795550770209786 1107 94-95 -0.7371195171240252 1107 96-97 -0.868139726458601 1107 98-99 -0.7980364669426727 1107 100 -0.8276498107851573 1108 1 -0.5846645500527607 1108 2 -0.45372371277211343 1108 3 -0.5228160959871708 1108 4 -0.4201625361050816 1108 5 -0.4140486535603287 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0.6309880520718494 2311 12-13 0.6482682975520291 2311 14-15 0.8501494790037754 2311 16-17 0.8384576383781308 2311 18-19 0.7626318955611069 2311 20-21 0.7989976799130218 2311 22-23 0.8653198100738564 2311 24-25 0.8654052146991091 2311 26-27 0.878954353633354 2311 28-29 0.9110056026763971 2311 30-31 0.9293436832242463 2311 32-33 0.9342094124942619 2311 34-35 0.9799228926378376 2311 36-37 1.0068486785331956 2311 38-39 1.0112425400300111 2311 40-41 1.055370945851017 2311 42-43 1.0374786620937968 2311 44-45 1.112510459757921 2311 46-47 1.0979257383163201 2311 48-49 1.1087071422647767 2311 50-51 1.0899523758791005 2311 52-53 0.9558335182216737 2311 54-55 0.9443466320165896 2311 56-57 0.9313647643216783 2311 58-59 0.8751726674718725 2311 60-61 0.8424164737376856 2311 62-63 0.8934696023487518 2311 64-65 0.9196672025564183 2311 66-67 0.8632181347655248 2311 68-69 0.7527190649008233 2311 70-71 0.7772015667704224 2311 72-73 0.7899962503364648 2311 74-75 0.8170499898985852 2311 76-77 0.8446334927726049 2311 78-79 0.9222984224407718 2311 80-81 0.9356673394731274 2311 82-83 0.8873619232704009 2311 84-85 0.9545521703745088 2311 86-87 1.0530279059970518 2311 88-89 1.0761452699890768 2311 90-91 1.0826549985381924 2311 92-93 1.0449236853333197 2311 94-95 1.1123769420290834 2311 96-97 1.1534946844461942 2311 98-99 1.1949083844785733 2311 100 1.1524279064170244 2312 1 0.45392883387681593 2312 2 0.4890568542769991 2312 3 0.4956886939152625 2312 4 0.3138010033130101 2312 5 0.4032627644535367 2312 6 0.39210625483534756 2312 7 0.45775857131545195 2312 8 0.43904535547591195 2312 9 0.5673813276757969 2312 10-11 0.5740038391828861 2312 12-13 0.6086391037728944 2312 14-15 0.7891786325075216 2312 16-17 0.8077115788628646 2312 18-19 0.7709962745021102 2312 20-21 0.7560764979983503 2312 22-23 0.7186372849751592 2312 24-25 0.7892603030322576 2312 26-27 0.8623374018924963 2312 28-29 0.8590189984365821 2312 30-31 0.8701294307731118 2312 32-33 0.9094543161899438 2312 34-35 0.8656749515070175 2312 36-37 0.90554591673272 2312 38-39 1.0384412193330803 2312 40-41 0.956591268977057 2312 42-43 0.8598118863479698 2312 44-45 0.9208313119178939 2312 46-47 0.9641993004641733 2312 48-49 0.9453265902403132 2312 50-51 1.0320837827066143 2312 52-53 0.8657907675808332 2312 54-55 0.8918922459854386 2312 56-57 0.8302306556872239 2312 58-59 0.8141698314136505 2312 60-61 0.8728260659913829 2312 62-63 0.8911662490035823 2312 64-65 0.8509958916346463 2312 66-67 0.8650094453516601 2312 68-69 0.9009431722347259 2312 70-71 0.9200433516411479 2312 72-73 0.9552813874615538 2312 74-75 0.9254779284282861 2312 76-77 1.0585424494304227 2312 78-79 1.0215713678718252 2312 80-81 0.9965483005444611 2312 82-83 0.9244708639858779 2312 84-85 1.012180055203693 2312 86-87 1.1425786466865198 2312 88-89 1.1128831317857788 2312 90-91 1.1325703573037487 2312 92-93 1.1893180652392594 2312 94-95 1.0941086995880624 2312 96-97 1.1889659693567722 2312 98-99 1.23989388953607 2312 100 1.3576913955237906 2313 1 0.4680420840132662 2313 2 0.46614872546676267 2313 3 0.4929888040015733 2313 4 0.37269301696741763 2313 5 0.39242527590158716 2313 6 0.4170676321168614 2313 7 0.4257683218173156 2313 8 0.47549456313200267 2313 9 0.5374609783599027 2313 10-11 0.6379052425520868 2313 12-13 0.6159614623952478 2313 14-15 0.7806820969885067 2313 16-17 0.7581121450210304 2313 18-19 0.7092080195089068 2313 20-21 0.7394044349438715 2313 22-23 0.6723737438963155 2313 24-25 0.750135951755162 2313 26-27 0.8129155863009387 2313 28-29 0.7928518060075618 2313 30-31 0.8768187931050946 2313 32-33 0.9022644774340165 2313 34-35 0.8959979484449505 2313 36-37 0.9552746326082726 2313 38-39 0.9604631070820489 2313 40-41 1.0193684505824692 2313 42-43 0.8712250152969716 2313 44-45 1.0270442502973154 2313 46-47 1.0039293577275572 2313 48-49 1.0569830191883796 2313 50-51 0.9595068379255451 2313 52-53 0.8933590627231567 2313 54-55 0.7873257455275109 2313 56-57 0.8026288258492187 2313 58-59 0.9029602233764251 2313 60-61 0.9139386778122471 2313 62-63 0.9044175418239249 2313 64-65 0.8890101288475236 2313 66-67 0.8502544105218348 2313 68-69 0.8549358824856981 2313 70-71 0.8484180983963725 2313 72-73 0.8600386887742602 2313 74-75 0.9128062076589814 2313 76-77 0.9132486392414201 2313 78-79 0.9297172923655701 2313 80-81 0.8854339772977369 2313 82-83 0.9010238673069715 2313 84-85 0.926343491746799 2313 86-87 0.926580746652288 2313 88-89 0.9639696559261353 2313 90-91 1.053049880324739 2313 92-93 1.1508105210890989 2313 94-95 1.0985875472896645 2313 96-97 1.1083864898152491 2313 98-99 1.1291530734172142 2313 100 1.160024485248762 2314 1 0.3168133116583576 2314 2 0.29543372068790674 2314 3 0.3293146274277241 2314 4 0.2548874227022324 2314 5 0.21864220319170613 2314 6 0.2869953303857926 2314 7 0.3178342019940956 2314 8 0.34956266997280494 2314 9 0.4358084256944039 2314 10-11 0.45565963363249296 2314 12-13 0.4789282736365479 2314 14-15 0.5954995004510195 2314 16-17 0.6119285336269087 2314 18-19 0.5924370970573847 2314 20-21 0.572796993601159 2314 22-23 0.5864813521667713 2314 24-25 0.6965442791199266 2314 26-27 0.7754285582280929 2314 28-29 0.6935362818232136 2314 30-31 0.7767813364402585 2314 32-33 0.7722224989086897 2314 34-35 0.7877633586780988 2314 36-37 0.7839186372921745 2314 38-39 0.8073988700827641 2314 40-41 0.7980178954296235 2314 42-43 0.7098056642960273 2314 44-45 0.7960513760705297 2314 46-47 0.698902426717801 2314 48-49 0.5967810358145442 2314 50-51 0.7746565356862476 2314 52-53 0.7787872832317433 2314 54-55 0.7521188023580478 2314 56-57 0.721050795814385 2314 58-59 0.6451638369578347 2314 60-61 0.600935822987509 2314 62-63 0.7324556628371752 2314 64-65 0.7762646269698479 2314 66-67 0.787319267515592 2314 68-69 0.7961161289046856 2314 70-71 0.6997173406805075 2314 72-73 0.7481566877164951 2314 74-75 0.7740496400329064 2314 76-77 0.7649134212658133 2314 78-79 0.7362150489687416 2314 80-81 0.802920091070888 2314 82-83 0.8383805476272848 2314 84-85 0.8911322452841048 2314 86-87 0.9149904368199806 2314 88-89 0.8228337074074545 2314 90-91 0.7914690377375244 2314 92-93 0.9674867270560021 2314 94-95 1.044503718016795 2314 96-97 1.0784642509242701 2314 98-99 0.9759620193673726 2314 100 1.0315259478880883 2315 1 0.329367904376074 2315 2 0.3226371782599742 2315 3 0.33427909121818544 2315 4 0.24785217635410106 2315 5 0.32483295219686426 2315 6 0.3019220769593076 2315 7 0.32699595992600194 2315 8 0.4002274602919016 2315 9 0.2168670516902722 2315 10-11 0.3775137095559771 2315 12-13 0.38592263637249147 2315 14-15 0.5318967674854918 2315 16-17 0.35854559382623563 2315 18-19 0.5493730059760651 2315 20-21 0.4269562757314418 2315 22-23 0.4242607636447673 2315 24-25 0.6028209471325425 2315 26-27 0.6695312821501389 2315 28-29 0.5897028882391027 2315 30-31 0.6422171432967261 2315 32-33 0.6408296338213191 2315 34-35 0.6485681656794924 2315 36-37 0.7714417657188619 2315 38-39 0.8086030339864649 2315 40-41 0.8228823194522263 2315 42-43 0.8295735954771999 2315 44-45 0.8152899578449109 2315 46-47 0.8685560576077194 2315 48-49 0.7946434488942913 2315 50-51 0.6762484127079702 2315 52-53 0.4895573667630444 2315 54-55 0.6679958081038322 2315 56-57 0.623930019211322 2315 58-59 0.6710642982226886 2315 60-61 0.792986914231129 2315 62-63 0.75861841461316 2315 64-65 0.7687735947038519 2315 66-67 0.6981744068123206 2315 68-69 0.7566870208063463 2315 70-71 0.6544397091253629 2315 72-73 0.7349144669365373 2315 74-75 0.44959762842059803 2315 76-77 0.6169996893284129 2315 78-79 0.7185250470496491 2315 80-81 0.6769437467211787 2315 82-83 0.6810071511727536 2315 84-85 0.701121312059577 2315 86-87 0.781793953773704 2315 88-89 0.6975614044632934 2315 90-91 0.8460009468037271 2315 92-93 0.9283584617402703 2315 94-95 0.9449221731212809 2315 96-97 0.9444200583350906 2315 98-99 1.0329915166410544 2315 100 1.0175417206705717 2316 1 0.13522493238501454 2316 2 0.12487639990130361 2316 3 0.1407121514623988 2316 4 0.06673236835858631 2316 5 0.03396562366039291 2316 6 -0.059159254164178776 2316 7 -0.022298872983867568 2316 8 0.03591234429098478 2316 9 0.11461217538315793 2316 10-11 0.10381770869028628 2316 12-13 0.08193328256239596 2316 14-15 -0.0025816509547098576 2316 16-17 0.08679662612361483 2316 18-19 0.20269485739547122 2316 20-21 0.27090353310265414 2316 22-23 0.17893528929401725 2316 24-25 0.16708539428331903 2316 26-27 0.041596729176923475 2316 28-29 0.04147027222904143 2316 30-31 -0.517626461838816 2316 32-33 -0.24674040701754052 2316 34-35 -0.2700085100734455 2316 36-37 -0.028827898271522656 2316 38-39 -0.032872730554608154 2316 40-41 0.1674513524498522 2316 42-43 0.11477391739644105 2316 44-45 0.0217455406209055 2316 46-47 -0.08721160892125113 2316 48-49 0.03478733254517152 2316 50-51 0.16669482184588702 2316 52-53 -0.1717362559470743 2316 54-55 -0.06989092233989425 2316 56-57 -0.05093320394136924 2316 58-59 -0.48824085135252204 2316 60-61 -0.07436896851078956 2316 62-63 -0.0596472588947492 2316 64-65 -0.2889991444219433 2316 66-67 -0.6281883261711343 2316 68-69 -0.6987382109117561 2316 70-71 -0.5016193321444078 2316 72-73 -0.6242499647395405 2316 74-75 -0.34214577848183936 2316 76-77 -0.0704430837527319 2316 78-79 0.05248728599273633 2316 80-81 -0.0050486186608722505 2316 82-83 0.1765860724694548 2316 84-85 0.1290202894478263 2316 86-87 0.24908301810647515 2316 88-89 0.3432587020665885 2316 90-91 -0.27019729169329665 2316 92-93 -0.3584616183037248 2316 94-95 0.05504185140782525 2316 96-97 0.30115853518168834 2316 98-99 0.35227760911069694 2316 100 0.37425836744468555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 188.0 1 442.0 2 236.0 3 267.0 4 246.0 5 241.0 6 248.0 7 672.0 8 1701.0 9 3020.0 10 4865.0 11 7879.0 12 11906.0 13 15476.0 14 18359.0 15 21034.0 16 25159.0 17 30495.0 18 36667.0 19 43881.0 20 51536.0 21 61555.0 22 68592.0 23 71217.0 24 71624.0 25 74402.0 26 80973.0 27 91784.0 28 106598.0 29 126250.0 30 150827.0 31 185597.0 32 232110.0 33 298415.0 34 406108.0 35 558351.0 36 808789.0 37 1131002.0 38 1013368.0 39 227406.0 40 197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.98015146822827 18.477202756436903 13.0500825573057 23.492563218029126 2 16.072434040684065 19.850640013591743 40.38633698404214 23.69058896168206 3 19.093568917139397 25.62462408953492 29.457817877838043 25.823989115487645 4 12.82078584998397 16.27366770619984 37.577120422454975 33.32842602136121 5 13.439152527889487 37.068806765150676 34.370013228503346 15.122027478456484 6 31.065856809055443 36.13578786359858 18.31763941569131 14.480715911654668 7 27.628449744265836 32.27925504556701 22.08245478291709 18.00984042725006 8 26.141282068057947 34.87792253395734 20.27642899912296 18.70436639886175 9 26.801609616357986 16.098830152879103 20.586490379136997 36.513069851625914 10-11 25.170003464210595 25.929686711303056 28.06702100497553 20.833288819510816 12-13 25.560621637392323 23.793138230659878 28.21370594873176 22.432534183216042 14-15 23.023133040759518 25.20546209562518 26.1659704271056 25.6054344365097 16-17 22.344153736070073 27.874973498350275 26.934272595969098 22.84660016961056 18-19 22.036810779264435 27.04668789032974 29.0341102156898 21.88239111471602 20-21 22.9805093408082 26.312816461999876 28.944996768549903 21.76167742864202 22-23 22.89924979773959 26.11062667266546 28.74849340011516 22.241630129479795 24-25 22.427599845385515 26.191274620144917 29.031760751149378 22.34936478332019 26-27 22.310554110232598 26.385693895424666 28.829334790556842 22.474417203785897 28-29 22.224175129510332 26.58553247995886 28.933317253599057 22.256975136931747 30-31 22.742693146311012 26.39847433516173 28.88662496350456 21.972207555022706 32-33 22.55243847382232 26.577009604185253 28.62732314135647 22.243228780635956 34-35 22.75065569321103 26.614497779211177 28.66443821666686 21.970408310910933 36-37 22.674739049555924 26.499171512931596 28.53981628432805 22.28627315318443 38-39 22.5394224468255 26.705904140458475 28.595171773137146 22.159501639578885 40-41 22.68120814652871 26.33009613200744 28.77475383867867 22.213941882785182 42-43 22.433300898841576 26.506454027607614 28.464095249557698 22.596149823993112 44-45 22.373511573792783 26.800224415851552 28.479632306572 22.346631703783668 46-47 22.36057233124835 26.533482769675025 28.23076152063903 22.875183378437594 48-49 22.547821330739357 26.606859750907695 28.15291593298283 22.692402985370116 50-51 22.504969671728738 26.863669349324415 27.99462325378131 22.63673772516554 52-53 22.422497035405726 27.001429383840716 27.84461259804492 22.73146098270864 54-55 22.300434447121063 26.906425397713097 27.990232311428976 22.80290784373686 56-57 22.5078814445201 26.920918836445324 27.68494267212031 22.886257046914267 58-59 22.364316621607294 26.906674088210348 27.940463647688716 22.788545642493638 60-61 22.553834119746377 27.05408856373656 27.805874475623405 22.586202840893655 62-63 22.50549467662045 26.94994961314859 27.82233876166133 22.722216948569628 64-65 22.32566403347688 26.719636936222802 27.89038343186052 23.064315598439798 66-67 22.40640917931404 26.721189716707254 27.726908317105053 23.14549278687365 68-69 22.134209471928745 26.734276716405326 27.901280668488624 23.230233143177305 70-71 22.23396713040048 26.504379297157122 27.94306106066489 23.31859251177751 72-73 22.263890494983958 26.794224485813032 27.646969848082364 23.29491517112064 74-75 22.31750257575844 26.849918037084464 27.559931991197324 23.272647395959776 76-77 22.192573830156267 26.721944374959623 27.554646822905365 23.53083497197875 78-79 22.108784001679275 26.687007071897 27.637357171015886 23.566851755407843 80-81 22.159880827168664 26.80031832658362 27.497575149651514 23.542225696596198 82-83 22.4042761727184 26.621805827016708 27.740770157372967 23.233147842891917 84-85 22.447014556821838 26.71618397124387 27.39249377789345 23.44430769404084 86-87 22.105118157326853 26.81352768280565 27.62469033049196 23.456663829375536 88-89 22.15834295443413 26.48278925411196 27.650964991508552 23.70790279994536 90-91 22.450983908965068 26.401216878665796 27.587120180023284 23.560679032345853 92-93 22.34440267343656 26.463254000084667 27.56951418054238 23.62282914593639 94-95 22.102368260016632 26.292320864327166 27.681805129034487 23.92350574662171 96-97 22.165209677888377 26.252201348368075 27.62508385152061 23.957505122222937 98-99 22.123303561071804 26.266798680961774 27.47081047939417 24.13908727857226 100 22.393294626380534 26.11771009707022 27.19667151816061 24.29232375838864 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2462.0 1 1781.0 2 2644.5 3 3816.5 4 2525.0 5 1788.0 6 2570.5 7 4599.0 8 7314.0 9 8499.5 10 7209.0 11 5680.5 12 5628.0 13 6015.0 14 6430.5 15 6928.0 16 7407.0 17 8005.5 18 8724.0 19 9342.5 20 9981.0 21 11061.5 22 12717.0 23 15037.0 24 17854.0 25 21503.5 26 26523.5 27 32572.5 28 39314.0 29 48382.0 30 59571.5 31 70299.5 32 82975.0 33 97427.0 34 110858.0 35 123851.0 36 139174.5 37 155686.0 38 165620.0 39 170633.5 40 174121.5 41 176062.0 42 180364.0 43 189009.0 44 198773.5 45 210849.5 46 222574.0 47 230245.0 48 237438.0 49 242330.5 50 241818.5 51 238833.5 52 233982.0 53 227326.5 54 219158.5 55 204508.0 56 185955.0 57 165468.5 58 145110.0 59 126828.5 60 106145.5 61 85252.0 62 68213.0 63 53711.0 64 40520.0 65 29634.0 66 21544.5 67 16231.0 68 12404.0 69 9235.0 70 7018.5 71 5300.0 72 4072.0 73 3049.5 74 2206.5 75 1596.5 76 1115.5 77 701.5 78 451.0 79 316.5 80 246.0 81 174.0 82 119.0 83 99.5 84 80.0 85 58.5 86 49.5 87 44.5 88 36.0 89 26.0 90 23.0 91 19.0 92 11.0 93 10.0 94 10.0 95 7.5 96 5.5 97 3.0 98 3.5 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02405755401401034 2 0.10940971570858934 3 0.01673928913156535 4 0.017798947395086796 5 0.019984492563599777 6 0.04531694792590936 7 0.04553219101068715 8 0.03854506933559261 9 0.03263416308438704 10-11 0.036963860520494204 12-13 0.035498551827968454 14-15 0.03130131167480148 16-17 0.038462283533755 18-19 0.03707976064306686 20-21 0.04833862969298223 22-23 0.04819789382985829 24-25 0.06665912763964599 26-27 0.05871996924341891 28-29 0.05131063997895254 30-31 0.04918304487172588 32-33 0.04436491120477681 34-35 0.04555702675123843 36-37 0.04095413616906715 38-39 0.04581366273693504 40-41 0.05457240057135449 42-43 0.05651786691453839 44-45 0.05906766961113688 46-47 0.06073166422807289 48-49 0.06039224244053868 50-51 0.05494493667962375 52-53 0.06400170340065861 54-55 0.050416553319106314 56-57 0.05551615871230328 58-59 0.05303258465717489 60-61 0.04952246665926009 62-63 0.05606254500443152 64-65 0.05427437168473909 66-67 0.05221300521898251 68-69 0.05794178270614534 70-71 0.05790866838541029 72-73 0.06041707818108997 74-75 0.05151760448354657 76-77 0.05286701305349966 78-79 0.062081072798025994 80-81 0.06198172983582085 82-83 0.052378576822657744 84-85 0.0586785763425001 86-87 0.05644335969288455 88-89 0.06464743265499198 90-91 0.07022719569884711 92-93 0.07499565788469362 94-95 0.07858028310426225 96-97 0.07386977097970208 98-99 0.08250433011136511 100 0.13020550913019774 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6039683.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.93724822081176 #Duplication Level Percentage of deduplicated Percentage of total 1 76.75429263411691 47.53949674892122 2 14.448804707205513 17.898384072884426 3 4.263904235008815 7.9228348498053425 4 1.6908712175749907 4.1891164124947355 5 0.8485036148122646 2.627698950344164 6 0.48077340292649856 1.7866668957013734 7 0.3181484469676413 1.3793667531630396 8 0.22738902906808742 1.1267080588863607 9 0.15557640692752786 0.8672377079855081 >10 0.7440510664800339 8.13053943475104 >50 0.04329401338351345 1.8348967740454254 >100 0.022818743330444348 2.528603140579698 >500 0.0010371699446825415 0.4297410095991848 >1k 4.014841898364713E-4 0.49348216043040244 >5k 3.345701581909496E-5 0.11343417091500617 >10k+ 1.0037104745728487E-4 1.1317928594930382 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24111 0.3992096936213374 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18721 0.3099665992403906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11785 0.19512613493125386 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.31143207350452E-5 0.0 0.0 0.0 3.31143207350452E-5 7 3.31143207350452E-5 0.0 0.0 0.0 3.31143207350452E-5 8 3.31143207350452E-5 0.0 0.0 0.0 3.31143207350452E-5 9 3.31143207350452E-5 0.0 0.0 0.0 8.2785801837613E-5 10-11 3.31143207350452E-5 0.0 0.0 8.2785801837613E-6 8.2785801837613E-5 12-13 3.31143207350452E-5 0.0 0.0 1.65571603675226E-5 8.2785801837613E-5 14-15 3.31143207350452E-5 0.0 0.0 3.31143207350452E-5 9.93429622051356E-5 16-17 6.62286414700904E-5 0.0 0.0 4.96714811025678E-5 1.324572829401808E-4 18-19 6.62286414700904E-5 0.0 0.0 5.79500612863291E-5 1.65571603675226E-4 20-21 6.62286414700904E-5 0.0 0.0 1.1590012257265821E-4 1.65571603675226E-4 22-23 6.62286414700904E-5 0.0 0.0 3.394217875342133E-4 1.904073442265099E-4 24-25 6.62286414700904E-5 0.0 0.0 8.940866598462204E-4 2.152430847777938E-4 26-27 6.62286414700904E-5 0.0 0.0 0.002011694984653996 2.152430847777938E-4 28-29 6.62286414700904E-5 0.0 0.0 0.005554927303303833 2.152430847777938E-4 30-31 6.62286414700904E-5 0.0 0.0 0.01872614837566806 2.235216649615551E-4 32-33 6.62286414700904E-5 0.0 0.0 0.05014336017304219 2.814717262478842E-4 34-35 6.62286414700904E-5 0.0 0.0 0.09704979549423373 3.31143207350452E-4 36-37 6.62286414700904E-5 0.0 0.0 0.17238487516646156 3.394217875342133E-4 38-39 6.62286414700904E-5 0.0 0.0 0.28122336884237137 3.642575280854972E-4 40-41 6.62286414700904E-5 0.0 0.0 0.40163531761517945 3.642575280854972E-4 42-43 6.62286414700904E-5 0.0 0.0 0.520888265162261 3.642575280854972E-4 44-45 6.62286414700904E-5 0.0 0.0 0.6678661777447591 3.725361082692585E-4 46-47 9.93429622051356E-5 0.0 0.0 0.8394314734730284 3.973718488205424E-4 48-49 9.93429622051356E-5 0.0 0.0 1.016187438976516 4.96714811025678E-4 50-51 9.93429622051356E-5 8.2785801837613E-6 0.0 1.1998642975136278 5.049933912094393E-4 52-53 9.93429622051356E-5 1.65571603675226E-5 0.0 1.3791535085533462 5.381077119444845E-4 54-55 9.93429622051356E-5 1.65571603675226E-5 0.0 1.5865898922178532 5.712220326795297E-4 56-57 9.93429622051356E-5 1.65571603675226E-5 0.0 1.821287640427486 5.960577732308136E-4 58-59 9.93429622051356E-5 1.65571603675226E-5 0.0 2.0823609451025824 6.126149335983362E-4 60-61 1.2417870275641948E-4 1.65571603675226E-5 0.0 2.34977564219844 6.126149335983362E-4 62-63 1.490144433077034E-4 1.65571603675226E-5 0.0 2.6232254242482593 6.126149335983362E-4 64-65 1.490144433077034E-4 1.65571603675226E-5 0.0 2.9014767828046604 6.208935137820976E-4 66-67 1.490144433077034E-4 1.65571603675226E-5 0.0 3.213132212402538 6.291720939658589E-4 68-69 1.490144433077034E-4 1.65571603675226E-5 0.0 3.5558074819489702 6.374506741496202E-4 70-71 1.821287640427486E-4 1.65571603675226E-5 0.0 3.919104694733151 6.457292543333814E-4 72-73 2.152430847777938E-4 1.65571603675226E-5 0.0 4.26804684947869 7.285150561709944E-4 74-75 2.152430847777938E-4 1.65571603675226E-5 0.0 4.620623300924899 7.285150561709944E-4 76-77 2.235216649615551E-4 1.65571603675226E-5 0.0 5.010825899306305 7.285150561709944E-4 78-79 2.318002451453164E-4 1.65571603675226E-5 0.0 5.4362869706903485 7.285150561709944E-4 80-81 2.318002451453164E-4 2.48357405512839E-5 0.0 5.876111378693219 7.616293769060396E-4 82-83 2.318002451453164E-4 3.31143207350452E-5 0.0 6.32315470861633 8.2785801837613E-4 84-85 2.318002451453164E-4 3.31143207350452E-5 0.0 6.765545476476166 8.2785801837613E-4 86-87 2.318002451453164E-4 3.31143207350452E-5 0.0 7.224675533467568 8.2785801837613E-4 88 2.318002451453164E-4 3.31143207350452E-5 0.0 7.587451195700171 8.444151787436526E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9745 0.0 53.286312 1 GTATCAA 18060 0.0 42.5041 1 TACCTGG 5875 0.0 36.425903 2 ATCAACG 22700 0.0 33.241745 3 CAACGCA 22760 0.0 32.98896 5 TATCAAC 23265 0.0 32.528183 2 TCAACGC 23295 0.0 32.473366 4 GTACCTG 6915 0.0 31.969372 1 AACGCAG 24300 0.0 31.401287 6 ACCTGGG 6965 0.0 30.289309 3 ACGCAGA 27535 0.0 27.337547 7 GTACATG 22625 0.0 27.23398 1 GTGGTAT 4540 0.0 26.833193 1 CGCAGAG 28405 0.0 26.434072 8 TACATGG 23110 0.0 26.091251 2 ACATGGG 23560 0.0 24.868828 3 TGGTATC 4865 0.0 23.782587 2 GCAGAGT 30905 0.0 23.553093 9 CATGGGG 16590 0.0 22.997137 4 ATGGGGG 10475 0.0 21.844347 5 >>END_MODULE