FastQCFastQC Report
Mon 6 Jun 2016
ERR1211323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211323_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6039683
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208690.3455313797098291No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175590.29072717889332933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113300.18759262696403106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107000.055.2651061
GTATCAA184700.038.698781
TACCTGG52450.035.871082
ATCAACG230500.030.3535733
CAACGCA231500.030.1541545
TATCAAC239050.029.538832
TCAACGC237350.029.517834
ACCTGGG62750.029.0896823
GTACCTG66450.028.956971
AACGCAG247250.028.4228176
GTGGTAT45550.027.7158471
GTACATG225050.026.5468881
TACATGG229800.025.5010432
ACGCAGA279500.024.925067
TGGTATC50000.024.4915352
ACATGGG233950.024.1895263
CGCAGAG288500.024.1475038
CATGGGG161100.022.4580824
ATGGGGG99150.022.4274985
GCAGAGT313350.021.887989
Produced by FastQC (version 0.11.5)