##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211323_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6039683 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.049472132891744 33.0 31.0 34.0 30.0 34.0 2 32.17957184176719 33.0 31.0 34.0 30.0 34.0 3 32.243603016913305 34.0 31.0 34.0 30.0 34.0 4 35.903445594743964 37.0 35.0 37.0 35.0 37.0 5 35.80362512403383 37.0 35.0 37.0 35.0 37.0 6 35.81877774048737 37.0 35.0 37.0 35.0 37.0 7 35.73263861696053 37.0 35.0 37.0 33.0 37.0 8 35.712263044269044 37.0 35.0 37.0 33.0 37.0 9 37.318930811434974 39.0 37.0 39.0 34.0 39.0 10-11 37.26289094974024 39.0 37.0 39.0 34.0 39.0 12-13 37.19070048875082 39.0 37.0 39.0 33.0 39.0 14-15 38.40769871531337 40.0 38.0 41.0 33.5 41.0 16-17 38.388272778554764 40.0 38.0 41.0 33.0 41.0 18-19 38.39755364644138 40.0 38.0 41.0 33.5 41.0 20-21 38.36415835069489 40.0 38.0 41.0 34.0 41.0 22-23 38.25012918724377 40.0 38.0 41.0 33.5 41.0 24-25 38.16032811655843 40.0 37.5 41.0 33.0 41.0 26-27 38.05830587466263 40.0 37.0 41.0 33.0 41.0 28-29 37.94149312141052 40.0 37.0 41.0 33.0 41.0 30-31 37.93836216238502 40.0 37.0 41.0 33.0 41.0 32-33 37.8631356976848 40.0 37.0 41.0 33.0 41.0 34-35 37.72114281494575 40.0 37.0 41.0 33.0 41.0 36-37 37.566813192016866 40.0 36.5 41.0 32.0 41.0 38-39 37.44661267818195 40.0 36.0 41.0 32.0 41.0 40-41 37.33482237395572 40.0 36.0 41.0 31.5 41.0 42-43 37.2216091473675 39.0 36.0 41.0 31.0 41.0 44-45 37.0594668627476 39.0 36.0 41.0 31.0 41.0 46-47 36.81318787757569 39.0 35.5 41.0 30.5 41.0 48-49 35.26579085359282 39.0 35.0 40.0 24.0 41.0 50-51 35.986053572679225 38.5 34.5 40.0 28.0 41.0 52-53 36.07914827649067 39.0 35.0 40.0 29.0 41.0 54-55 35.91112016971751 38.0 35.0 40.0 28.0 41.0 56-57 35.70220241691493 38.0 34.5 40.0 28.0 41.0 58-59 35.466235314005715 38.0 34.0 40.0 28.0 41.0 60-61 35.209891396618005 37.5 34.0 40.0 27.0 41.0 62-63 34.887862906049875 37.0 34.0 40.0 26.5 41.0 64-65 34.5502272221903 36.5 33.0 39.0 26.0 41.0 66-67 34.208677922334665 36.0 33.0 39.0 26.0 40.0 68-69 33.84607784878776 35.5 33.0 39.0 26.0 40.0 70-71 33.48309911298325 35.0 32.0 38.0 25.5 40.0 72-73 33.10341693429937 35.0 32.0 37.0 25.0 39.5 74-75 32.73367410176991 35.0 31.5 37.0 24.5 39.0 76-77 31.808212864814262 34.0 30.5 36.0 23.5 38.0 78-79 32.25335667451421 34.5 31.0 36.0 25.0 38.0 80-81 32.13100248804449 34.5 31.0 35.5 24.5 37.0 82-83 31.8596803838877 34.0 31.0 35.0 24.0 37.0 84-85 31.574839937791438 34.0 31.0 35.0 23.5 36.5 86-87 31.310510998673273 34.0 31.0 35.0 23.0 36.0 88-89 31.081861415574295 34.0 30.5 35.0 22.5 36.0 90-91 30.858843833360126 34.0 30.0 35.0 20.0 35.0 92-93 30.669182720351383 34.0 30.0 35.0 20.0 35.0 94-95 30.46982101544071 34.0 29.5 35.0 20.0 35.0 96-97 30.271119196156487 34.0 29.0 35.0 19.0 35.0 98-99 30.047179214538247 34.0 29.0 35.0 19.0 35.0 100 27.612259782508453 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.31411926109293375 1101 2 -0.2644885815728415 1101 3 -0.30305374655787176 1101 4 -0.16882818564224067 1101 5 -0.2100997075690998 1101 6 -0.2896583216442039 1101 7 -0.20744473003352226 1101 8 -0.20720190391939042 1101 9 -0.3819445719076029 1101 10-11 -0.30529164753145466 1101 12-13 -0.3679862209839371 1101 14-15 -0.5021133822163222 1101 16-17 -0.5209472516165548 1101 18-19 -0.5499329469146161 1101 20-21 -0.5976460324070914 1101 22-23 -0.6067558346316275 1101 24-25 -0.4676395941207758 1101 26-27 -0.6381461545736116 1101 28-29 -0.6254001987285847 1101 30-31 -0.5745947560186622 1101 32-33 -0.5580805451286466 1101 34-35 -0.5908809753194717 1101 36-37 -0.5593145144653988 1101 38-39 -0.5249501278576929 1101 40-41 -0.5645307952171592 1101 42-43 -0.5952259409504066 1101 44-45 -0.5362897008793368 1101 46-47 -0.4916641022735675 1101 48-49 0.6412297258779418 1101 50-51 -0.22688994732295242 1101 52-53 -0.48250771913549073 1101 54-55 -0.6239126897002123 1101 56-57 -0.5926453843013135 1101 58-59 -0.6188611893117795 1101 60-61 -0.6750904784257301 1101 62-63 -0.6688165040644094 1101 64-65 -0.6645831428064639 1101 66-67 -0.699080053721481 1101 68-69 -0.8106036296527392 1101 70-71 -0.6530828175247478 1101 72-73 -0.8266770713509857 1101 74-75 -0.9197114229572421 1101 76-77 -0.8502521757115957 1101 78-79 -0.6907280961371605 1101 80-81 -0.565354526910852 1101 82-83 -0.6250663564085777 1101 84-85 -0.5827523437095472 1101 86-87 -0.6417084496922492 1101 88-89 -0.6524716469522609 1101 90-91 -0.7540537176981665 1101 92-93 -0.8216788253382994 1101 94-95 -0.8659244066459486 1101 96-97 -0.9357660169575723 1101 98-99 -1.123449588074518 1101 100 -0.8810940998359449 1102 1 -0.31237715377038455 1102 2 -0.31469603844955785 1102 3 -0.33532281579208956 1102 4 -0.24369404030532138 1102 5 -0.27412941685556547 1102 6 -0.26341968814792693 1102 7 -0.25946253684620046 1102 8 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1105 68-69 -0.7849215049510079 1105 70-71 -0.8523856948753732 1105 72-73 -0.7616714555781101 1105 74-75 -0.8735858551777937 1105 76-77 -0.7879017605052567 1105 78-79 -0.614486198411889 1105 80-81 -0.6144657011712127 1105 82-83 -0.5340784314479343 1105 84-85 -0.5615184073008486 1105 86-87 -0.6518357856507855 1105 88-89 -0.569109896454961 1105 90-91 -0.7147796183643891 1105 92-93 -0.712735988944484 1105 94-95 -0.7399053355714642 1105 96-97 -0.6999671563505281 1105 98-99 -0.7891791649294184 1105 100 -0.7038260267451939 1106 1 -0.3134656515863199 1106 2 -0.2726176218735681 1106 3 -0.355784656644623 1106 4 -0.2139501845720133 1106 5 -0.26351439783564246 1106 6 -0.4174248231789335 1106 7 -0.3793331555686592 1106 8 -0.4070515147177076 1106 9 -0.49669312421260514 1106 10-11 -0.4324308974553688 1106 12-13 -0.5159342505074349 1106 14-15 -0.6405420670335715 1106 16-17 -0.6525600829717817 1106 18-19 -0.6166017133717219 1106 20-21 -0.6958610889655006 1106 22-23 -0.6830935061320105 1106 24-25 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1.015351904610462 2205 46-47 0.9588755563261273 2205 48-49 2.2362165580564906 2205 50-51 1.4440156268092252 2205 52-53 1.1993431461312056 2205 54-55 1.0822256953160831 2205 56-57 1.0858625167118632 2205 58-59 1.0568723991710058 2205 60-61 1.047248848634851 2205 62-63 1.0923857533740247 2205 64-65 1.1032692488299318 2205 66-67 1.0700971811192872 2205 68-69 1.070417715179751 2205 70-71 1.085029948482223 2205 72-73 1.0179254724846274 2205 74-75 1.0721306776417947 2205 76-77 1.0438795755328414 2205 78-79 0.9542919999250472 2205 80-81 0.785063617883722 2205 82-83 0.8577117229323257 2205 84-85 0.9176301562525531 2205 86-87 0.9234923123394942 2205 88-89 0.9726511640962805 2205 90-91 1.0876087814183215 2205 92-93 0.9628733064436936 2205 94-95 0.9982964080997903 2205 96-97 1.0075956414811174 2205 98-99 1.0212565003425986 2205 100 1.2042425759366928 2206 1 0.5552631383832178 2206 2 0.5179516732656353 2206 3 0.5115902080642911 2206 4 0.3199206160617507 2206 5 0.40207566605718625 2206 6 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1.0190299296654146 2206 72-73 1.0755552895112075 2206 74-75 1.1559491898786902 2206 76-77 1.0780596753821499 2206 78-79 0.9674086108193407 2206 80-81 0.9484629295370155 2206 82-83 0.9545874555137779 2206 84-85 1.0521430435444863 2206 86-87 0.973274801801427 2206 88-89 0.998660239373006 2206 90-91 0.9773004614883369 2206 92-93 1.0348562316715366 2206 94-95 1.006377606371533 2206 96-97 1.0685297139200784 2206 98-99 1.1193520586264007 2206 100 1.280787760362756 2207 1 0.5423267610069047 2207 2 0.5259387447891584 2207 3 0.49053876024940024 2207 4 0.3601601149033442 2207 5 0.39655888460065114 2207 6 0.42328102242210264 2207 7 0.4573912083306553 2207 8 0.42183027004579543 2207 9 0.5917764415609454 2207 10-11 0.5782297307047202 2207 12-13 0.5908113121231793 2207 14-15 0.7461687387692848 2207 16-17 0.7328224918878092 2207 18-19 0.737559366857063 2207 20-21 0.7286840342559202 2207 22-23 0.765859758607391 2207 24-25 0.7234028912153363 2207 26-27 0.7407641384360275 2207 28-29 0.8178934995511469 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1.1849559056367553 2207 98-99 1.201161109814528 2207 100 1.3280785733551248 2208 1 0.5851653596233675 2208 2 0.5245094063014548 2208 3 0.4929691904565203 2208 4 0.3498921784690978 2208 5 0.4295928597095866 2208 6 0.4405416114179985 2208 7 0.5011044720810034 2208 8 0.4820034629537133 2208 9 0.6418876776959905 2208 10-11 0.6304423718057066 2208 12-13 0.6560019489271269 2208 14-15 0.8119251654226503 2208 16-17 0.7983426877759499 2208 18-19 0.7635568200700078 2208 20-21 0.7892802297096253 2208 22-23 0.790172937127096 2208 24-25 0.7709570821425515 2208 26-27 0.8057439386539684 2208 28-29 0.8134720024007862 2208 30-31 0.8506751119105047 2208 32-33 0.9179317576848192 2208 34-35 0.9593697735287137 2208 36-37 0.9966337993051937 2208 38-39 0.9514674574528001 2208 40-41 0.9946889298756716 2208 42-43 0.9942655398182154 2208 44-45 1.0304765723474532 2208 46-47 0.985118872836118 2208 48-49 2.2642603292236103 2208 50-51 1.4260882539833233 2208 52-53 1.1162234514586018 2208 54-55 1.1276505315329786 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2209 82-83 0.9996783551675001 2209 84-85 0.9511914191641431 2209 86-87 0.9590264850125756 2209 88-89 1.055347085338429 2209 90-91 1.1623528113240091 2209 92-93 1.1168329875736625 2209 94-95 1.1764944421524213 2209 96-97 1.2301143211653667 2209 98-99 1.1824924510921555 2209 100 1.2617637802782617 2210 1 0.5661735035193871 2210 2 0.4753482677373242 2210 3 0.4527432761007475 2210 4 0.3328660819114049 2210 5 0.4078798305143536 2210 6 0.40261877218159725 2210 7 0.44731756002335743 2210 8 0.47634493391452537 2210 9 0.6214347680701593 2210 10-11 0.5972823315714919 2210 12-13 0.5985371176834349 2210 14-15 0.7406988317833267 2210 16-17 0.776799614363803 2210 18-19 0.7753206035872466 2210 20-21 0.7953675575268022 2210 22-23 0.8234108060514416 2210 24-25 0.7595912520997246 2210 26-27 0.8568547600041043 2210 28-29 0.833588393648256 2210 30-31 0.81015828280281 2210 32-33 0.8234062226383827 2210 34-35 0.8979915542622265 2210 36-37 0.9490326890541496 2210 38-39 0.9572007490485603 2210 40-41 0.9626791905272754 2210 42-43 0.9410560011503009 2210 44-45 0.9571716658878273 2210 46-47 0.9848629528885269 2210 48-49 2.204917296987581 2210 50-51 1.3282890899012756 2210 52-53 1.0823067099033281 2210 54-55 1.083503860213419 2210 56-57 1.0226575398901403 2210 58-59 0.9349247208509652 2210 60-61 1.0102393963267389 2210 62-63 1.0486749986228787 2210 64-65 1.1195537236315758 2210 66-67 1.0669333682584394 2210 68-69 1.1252602509485499 2210 70-71 1.0740974554833187 2210 72-73 1.114940427780546 2210 74-75 1.0820306125150765 2210 76-77 1.0947341175711642 2210 78-79 1.0117782675937974 2210 80-81 1.0076223588712523 2210 82-83 0.9993703315140827 2210 84-85 0.9795915176661332 2210 86-87 0.9092612572392333 2210 88-89 0.9882927337757685 2210 90-91 1.133006467437717 2210 92-93 1.1137752226252609 2210 94-95 1.1021349602273034 2210 96-97 1.1603712182207495 2210 98-99 1.2338724052570882 2210 100 1.5684458605605798 2211 1 0.5477690389950993 2211 2 0.4951725679041985 2211 3 0.4970714799009244 2211 4 0.30100190072614197 2211 5 0.411729262077003 2211 6 0.4069503787088564 2211 7 0.4254436554539964 2211 8 0.45806045922271466 2211 9 0.5931553415699256 2211 10-11 0.6158199272846261 2211 12-13 0.6170486034626705 2211 14-15 0.7445125286135621 2211 16-17 0.759947943892108 2211 18-19 0.766925929525911 2211 20-21 0.6971581664269308 2211 22-23 0.7330806083832826 2211 24-25 0.7657167909791838 2211 26-27 0.790422296768142 2211 28-29 0.8239640321550539 2211 30-31 0.8315766101876321 2211 32-33 0.8680378159429765 2211 34-35 0.9245030363826103 2211 36-37 0.8995804945746784 2211 38-39 0.9115457459370191 2211 40-41 0.9319737151552232 2211 42-43 0.854245888058415 2211 44-45 0.8876545512784304 2211 46-47 0.9821145088383361 2211 48-49 2.2491836561214384 2211 50-51 1.4351883731434398 2211 52-53 1.1760761824613226 2211 54-55 1.1221505769527553 2211 56-57 1.044093368756549 2211 58-59 1.1050872152979068 2211 60-61 1.1516099310478154 2211 62-63 1.1080034255110505 2211 64-65 1.1162693399027503 2211 66-67 1.117679229163123 2211 68-69 1.1292498671426827 2211 70-71 1.0836982174177408 2211 72-73 1.1651381325252643 2211 74-75 1.1904643041070528 2211 76-77 0.9844631058076629 2211 78-79 0.7792194341071834 2211 80-81 0.8366394596075182 2211 82-83 0.9220800995259388 2211 84-85 0.9940138259773939 2211 86-87 1.042571973481774 2211 88-89 1.0596850477795776 2211 90-91 1.0722690798113028 2211 92-93 1.0915001560469655 2211 94-95 1.0698779815623425 2211 96-97 1.1313291966756331 2211 98-99 1.1918295676876092 2211 100 1.491004243805829 2212 1 0.579561572164117 2212 2 0.5321297985943829 2212 3 0.5236950355767576 2212 4 0.3706970469204691 2212 5 0.4029348001560038 2212 6 0.3706733359155905 2212 7 0.28490524521376415 2212 8 0.36179794990379577 2212 9 0.5439071985197472 2212 10-11 0.5571239507962105 2212 12-13 0.6153661341768455 2212 14-15 0.781958167593416 2212 16-17 0.7179457832229801 2212 18-19 0.7486562034369584 2212 20-21 0.7637113933568855 2212 22-23 0.7797746730143444 2212 24-25 0.7968996738761831 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0.4240737280272313 2311 12-13 0.4746081110201814 2311 14-15 0.4701924798560668 2311 16-17 0.552717355110623 2311 18-19 0.6981163567777529 2311 20-21 0.6677645468273994 2311 22-23 0.7025559438211246 2311 24-25 0.6156241946938721 2311 26-27 0.6861282015580983 2311 28-29 0.787058149044185 2311 30-31 0.6417440504499936 2311 32-33 0.6467704790653173 2311 34-35 0.7621123138043586 2311 36-37 0.7731141651609619 2311 38-39 0.843224617315613 2311 40-41 0.7311859053462513 2311 42-43 0.7593333151087975 2311 44-45 0.812106515229793 2311 46-47 0.8550960274174102 2311 48-49 -5.964945074700999 2311 50-51 -1.0224104021927118 2311 52-53 0.36190143471078073 2311 54-55 0.816539221611059 2311 56-57 0.8739679675145027 2311 58-59 0.9377005882262495 2311 60-61 0.8259183221835258 2311 62-63 0.6839080416225016 2311 64-65 0.7428618579592836 2311 66-67 0.8648423878059717 2311 68-69 0.8951891595659518 2311 70-71 0.8950532012044405 2311 72-73 0.4222091448260912 2311 74-75 0.726109476777907 2311 76-77 0.8237403434830526 2311 78-79 0.7051552394698959 2311 80-81 0.7767640223188792 2311 82-83 0.804274922928311 2311 84-85 0.8817092573145153 2311 86-87 0.8910542840633191 2311 88-89 0.9020087083546322 2311 90-91 0.9857532668609146 2311 92-93 0.9909135936035263 2311 94-95 1.0013164400566055 2311 96-97 1.048226408152047 2311 98-99 1.072124965688019 2311 100 1.2728420781060272 2312 1 0.12332562633545052 2312 2 0.2550670463649354 2312 3 0.22965770089246007 2312 4 0.04187766242438329 2312 5 0.23805965307685995 2312 6 0.12093491730890804 2312 7 0.23332737633548106 2312 8 0.288250016489215 2312 9 0.2653670596173825 2312 10-11 0.2176223248916287 2312 12-13 0.3302353371790474 2312 14-15 0.3097031549302187 2312 16-17 0.46185944750067875 2312 18-19 0.32781127075205063 2312 20-21 0.3476495102649011 2312 22-23 0.6196115196542138 2312 24-25 0.6653276881638135 2312 26-27 0.5527316232875066 2312 28-29 0.709517653405257 2312 30-31 0.7368499621210489 2312 32-33 0.7547601081542155 2312 34-35 0.3033314412549615 2312 36-37 0.6515618008701267 2312 38-39 0.7835011539683094 2312 40-41 0.5353846140167917 2312 42-43 0.580439293905485 2312 44-45 0.6842341672169283 2312 46-47 0.7691274883649797 2312 48-49 -4.608308132106011 2312 50-51 -0.7960492201102269 2312 52-53 0.4903940655743142 2312 54-55 0.787646459013466 2312 56-57 0.9114424283068345 2312 58-59 0.9613719201247761 2312 60-61 0.9435524371110091 2312 62-63 0.7377782542010038 2312 64-65 0.9161021173998734 2312 66-67 0.9375423907427205 2312 68-69 0.8127612881618873 2312 70-71 0.821307716452452 2312 72-73 0.9163731998859035 2312 74-75 0.6739088046824548 2312 76-77 0.7794302901601213 2312 78-79 0.5059342115246537 2312 80-81 0.6718996161080781 2312 82-83 0.6902565724569634 2312 84-85 0.7477069750428136 2312 86-87 0.8315305194897071 2312 88-89 0.8719993527188059 2312 90-91 0.7118447926686251 2312 92-93 0.9938458302717486 2312 94-95 1.0315962910376903 2312 96-97 1.0676785790371426 2312 98-99 1.0783792921451685 2312 100 0.6793658510703402 2313 1 0.059228102814913086 2313 2 0.22853902061028464 2313 3 0.2699118245036587 2313 4 0.07183825337510541 2313 5 0.18343972837959654 2313 6 0.27791753477284686 2313 7 0.21650359769222405 2313 8 0.2811156834364965 2313 9 0.4050707516717367 2313 10-11 0.26058836465221447 2313 12-13 0.07677501913136808 2313 14-15 0.15878152898314823 2313 16-17 0.16608851953782988 2313 18-19 0.47372096297292643 2313 20-21 0.40739076117843354 2313 22-23 0.5405737110193769 2313 24-25 0.28934477466945197 2313 26-27 0.44546014489309727 2313 28-29 0.3900404708747516 2313 30-31 0.5718254933568758 2313 32-33 0.5533738532165202 2313 34-35 0.41054163210207406 2313 36-37 -0.34369431169544384 2313 38-39 0.2199094261682788 2313 40-41 0.4917918223574489 2313 42-43 0.5299351171013171 2313 44-45 0.47577625441638816 2313 46-47 0.4040088911791173 2313 48-49 -5.190734795195642 2313 50-51 -1.299624481218295 2313 52-53 0.11511514941567924 2313 54-55 0.39503010518310333 2313 56-57 0.42643613766047395 2313 58-59 0.4788129684424902 2313 60-61 0.704226183838486 2313 62-63 0.790496646015761 2313 64-65 0.8753176830492464 2313 66-67 0.8526829589361071 2313 68-69 0.9389782507047926 2313 70-71 0.9365745601206399 2313 72-73 0.7020453276970358 2313 74-75 0.8069643011347125 2313 76-77 0.8259315806677279 2313 78-79 0.7793088195156272 2313 80-81 -0.15586456791182712 2313 82-83 0.28125354739614394 2313 84-85 0.6439224111788739 2313 86-87 0.8167107280061323 2313 88-89 0.8079554847113677 2313 90-91 0.848203803556995 2313 92-93 0.8978924273095892 2313 94-95 0.8147833822613109 2313 96-97 0.7706707989877444 2313 98-99 1.0466062087108448 2313 100 1.1345653478186293 2314 1 0.0889622977996467 2314 2 0.19706761617389645 2314 3 0.2012852259403104 2314 4 0.09389925621935902 2314 5 0.22406085491530803 2314 6 0.31172480680199044 2314 7 0.13134090438629187 2314 8 0.272125323293686 2314 9 0.4708415647595814 2314 10-11 0.33640068230266706 2314 12-13 0.27550416257025745 2314 14-15 0.2937173526703063 2314 16-17 0.5113456779360064 2314 18-19 0.5647884751186254 2314 20-21 0.6059654464135491 2314 22-23 0.39875876287585754 2314 24-25 0.48526969209024884 2314 26-27 0.5680369704861832 2314 28-29 0.6767359131628297 2314 30-31 0.5792483339082963 2314 32-33 0.5728903488002643 2314 34-35 0.33291410530836885 2314 36-37 0.4582611087487152 2314 38-39 0.5697655240428574 2314 40-41 0.33446248135936685 2314 42-43 0.3255516265036391 2314 44-45 0.5424744972476532 2314 46-47 0.6418633164136622 2314 48-49 -6.353847862363683 2314 50-51 -1.4202273361711377 2314 52-53 -0.8851390250989937 2314 54-55 -0.5029122321287929 2314 56-57 -0.15514343355378912 2314 58-59 0.4177057643213189 2314 60-61 0.6814327805027744 2314 62-63 0.6088302803794647 2314 64-65 0.7631982202667729 2314 66-67 0.8127431337867108 2314 68-69 0.8388745033089862 2314 70-71 0.639302463730715 2314 72-73 0.7542906299819734 2314 74-75 0.8948752776468183 2314 76-77 0.771255578216568 2314 78-79 0.6198189250892128 2314 80-81 0.6966883938187252 2314 82-83 0.7168576865317355 2314 84-85 0.7342151103757253 2314 86-87 0.7529952478234598 2314 88-89 0.676020517519671 2314 90-91 0.7861438703312942 2314 92-93 0.8276925647587774 2314 94-95 0.9851888301424658 2314 96-97 0.975764012722351 2314 98-99 1.1268028616053734 2314 100 -0.49944388676841456 2315 1 0.14261308339687417 2315 2 0.23862884399298423 2315 3 0.34803578328929774 2315 4 0.19096236590606708 2315 5 0.2805675622471355 2315 6 0.32172895975954674 2315 7 0.3633948073325186 2315 8 0.325912759516541 2315 9 0.5074027420760672 2315 10-11 0.2854204563000309 2315 12-13 0.45347069673221085 2315 14-15 0.22380326345641066 2315 16-17 0.3612713745253444 2315 18-19 0.5064191909846798 2315 20-21 0.4595267132253369 2315 22-23 0.4833250935710822 2315 24-25 0.5656894661346001 2315 26-27 0.6187251622983325 2315 28-29 0.6644550914879588 2315 30-31 0.6081696431140742 2315 32-33 0.6459253809234369 2315 34-35 0.6815389783765582 2315 36-37 0.5356189574509287 2315 38-39 0.5797492997906701 2315 40-41 0.6249318841861466 2315 42-43 0.6926749540749739 2315 44-45 0.6708261959083046 2315 46-47 0.5687040244177624 2315 48-49 -4.810770440946307 2315 50-51 -0.9136070332354294 2315 52-53 0.19115351247317136 2315 54-55 0.21366309531006777 2315 56-57 0.5810590520195049 2315 58-59 0.6448971482497967 2315 60-61 0.5495897669965046 2315 62-63 0.3324610258423917 2315 64-65 0.5430142181733757 2315 66-67 -0.21065298601671856 2315 68-69 0.5270053306330951 2315 70-71 0.5606298739318163 2315 72-73 0.6052310470206521 2315 74-75 0.812095857947476 2315 76-77 0.6934095867014776 2315 78-79 0.5949784017132131 2315 80-81 0.41564621052813067 2315 82-83 0.5108702574497066 2315 84-85 0.5652861623588628 2315 86-87 0.5603331926309387 2315 88-89 0.47655323768103486 2315 90-91 0.5915901406207134 2315 92-93 0.6626713724606326 2315 94-95 0.3251253190188592 2315 96-97 0.46742048491174515 2315 98-99 0.38832326688517327 2315 100 0.4826935216310346 2316 1 0.25460004466232533 2316 2 0.3008863915498239 2316 3 0.1966761932690133 2316 4 -0.013210839757334725 2316 5 0.20913655268002884 2316 6 0.1819290190724061 2316 7 0.24485624226974068 2316 8 0.3378553349240434 2316 9 0.5060284646813002 2316 10-11 0.41328769988309233 2316 12-13 0.35959772713678717 2316 14-15 0.4608607641216338 2316 16-17 0.4527998315016859 2316 18-19 0.15622237116851778 2316 20-21 0.4800646742836534 2316 22-23 0.6459395432520694 2316 24-25 0.6288112576543128 2316 26-27 0.6684871939691917 2316 28-29 0.6963632340639094 2316 30-31 0.6253065336498977 2316 32-33 0.5627961127995036 2316 34-35 0.5396471357836603 2316 36-37 0.3653201462059599 2316 38-39 0.5117760680759318 2316 40-41 0.08896022814882798 2316 42-43 0.08124731017298359 2316 44-45 0.32128697768428083 2316 46-47 0.37809716229096324 2316 48-49 -5.025660529954514 2316 50-51 -1.0287651352859015 2316 52-53 0.2391304075714018 2316 54-55 0.5092719899222402 2316 56-57 0.44182584231362654 2316 58-59 0.3947554826203472 2316 60-61 0.5212898361191947 2316 62-63 0.435434074368402 2316 64-65 0.46767355216563544 2316 66-67 0.5744050394309212 2316 68-69 0.3278598879903356 2316 70-71 -0.14709024904660595 2316 72-73 -0.02006731930944028 2316 74-75 0.1413975524638147 2316 76-77 0.15910857672221823 2316 78-79 0.31838298186976743 2316 80-81 0.23054654167429334 2316 82-83 0.11251737358465874 2316 84-85 0.26585242778840623 2316 86-87 0.36047976904864143 2316 88-89 0.15413126312320458 2316 90-91 0.18636372785265465 2316 92-93 0.39714703671247875 2316 94-95 0.5039039397647578 2316 96-97 0.547554543856343 2316 98-99 0.5699693145984739 2316 100 -0.2831088639068611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 6.0 11 11.0 12 39.0 13 160.0 14 632.0 15 1799.0 16 4082.0 17 8378.0 18 14172.0 19 20726.0 20 28548.0 21 38869.0 22 47764.0 23 51911.0 24 53371.0 25 58800.0 26 68934.0 27 84241.0 28 103097.0 29 126976.0 30 155226.0 31 192345.0 32 241213.0 33 313021.0 34 428275.0 35 602322.0 36 915343.0 37 1323250.0 38 1032090.0 39 124066.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.82465327808307 19.35980245048841 13.971858137986546 23.84368613344198 2 15.749599764088892 20.282063453928703 39.94206203329612 24.026274748686287 3 18.749387384762105 25.506566573063473 30.137192698950937 25.606853343223484 4 12.8313489848838 16.705738684709353 37.49703150922542 32.96588082118143 5 13.284251002707443 37.24460432131144 34.43761954339061 15.033525132590508 6 31.149050943096324 36.51665320016954 17.97717759881318 14.35711825792095 7 27.60757476841086 32.377642998813016 21.863332893464772 18.151449339311352 8 26.196544245702498 35.320743632598685 19.94585916494824 18.536852956750582 9 26.53486076195024 16.077658822980048 20.425505068485574 36.96197534658413 10-11 25.19346415834109 26.084041711233485 27.930772134670722 20.7917219957547 12-13 25.467827170227814 23.786379635864634 28.22606242022545 22.519730773682102 14-15 22.850722691972443 25.279851541692928 26.08006837453436 25.789357391800273 16-17 22.311801363072853 27.977159319746757 26.887735862724117 22.82330345445627 18-19 21.993149309326334 27.107788604137006 28.996505280161227 21.902556806375433 20-21 22.85200469296542 26.395107487518377 28.950649355810025 21.80223846370618 22-23 22.7743331896996 26.145321741635613 28.778438548148554 22.301906520516237 24-25 22.3505475950555 26.221314300984723 29.05989090154084 22.368247202418935 26-27 22.255664954323723 26.420026034500516 28.863045347781785 22.461263663393975 28-29 22.16119472278669 26.63189299604098 28.97265013220187 22.23426214897046 30-31 22.675088660005137 26.38995467260601 28.915749628135885 22.019207039252972 32-33 22.490766568740288 26.592306866274978 28.648297197883675 22.26862936710106 34-35 22.676070647601357 26.60392929940541 28.72524853620676 21.994751516786476 36-37 22.615682405754846 26.507219473757687 28.584717506737384 22.292380613750083 38-39 22.5158792747849 26.685569658321928 28.63974639530443 22.15880467158874 40-41 22.58987298763522 26.369264093921725 28.81226682332651 22.228596095116547 42-43 22.36475498936896 26.503417314759055 28.561513900987702 22.570313794884285 44-45 22.30914036071506 26.787796978164202 28.506426449379784 22.396636211740955 46-47 22.315223571263395 26.49815643551093 28.271576634499812 22.915043358725864 48-49 22.540712332477128 26.680361253525636 28.12514742951225 22.65377898448499 50-51 22.44027305873727 26.81771644301623 28.044071272818204 22.697939225428296 52-53 22.32338621534793 26.977279501871294 27.890504421554663 22.808829861226112 54-55 22.232244811178287 26.904808899816135 27.99275426153675 22.870192027468832 56-57 22.494452538717976 26.892656686286653 27.678780921270178 22.93410985372519 58-59 22.233760801687584 26.89120449771123 27.991233218590565 22.88380148201062 60-61 22.46940138506249 27.002409938194678 27.85356709184647 22.67462158489636 62-63 22.44064601692908 26.955914336101305 27.80429733575516 22.799142311214457 64-65 22.206121076001118 26.707316517797075 27.8923005268224 23.194261879379404 66-67 22.295895037340802 26.7492327385425 27.719085006656968 23.235787217459723 68-69 22.042228742596986 26.73035666714944 27.879620334327786 23.347794255925784 70-71 22.104358997056476 26.530959356619267 27.921869991494937 23.442811654829327 72-73 22.11722565394776 26.852752960227267 27.599041823625363 23.430979562199614 74-75 22.15915479174391 26.863021757946143 27.502650631457165 23.47517281885279 76-77 22.03906122181324 26.782150443761605 27.50296083942116 23.675827495003997 78-79 21.931097313658427 26.690462318799423 27.6069001540519 23.77154021349025 80-81 21.919948459493025 26.87264214557395 27.423654340828158 23.783755054104866 82-83 22.15094415858334 26.669199149708334 27.645226283265956 23.534630408442375 84-85 22.169763512674276 26.772851735937337 27.310188937471413 23.747195813916978 86-87 21.8242599440956 26.876803733208664 27.5440809976973 23.754855324998434 88-89 21.852676101872664 26.55814722286815 27.550617674084588 24.038559001174598 90-91 22.060052659060652 26.474244208008425 27.543315215600252 23.922387917330674 92-93 21.937213358203223 26.5792124918197 27.490148861646237 23.993425288330847 94-95 21.616009715060546 26.405311690146 27.637717921150575 24.34096067364288 96-97 21.641089622245136 26.405129952193356 27.611302883176524 24.342477542384984 98-99 21.554336515931467 26.411057500636105 27.491012381836594 24.543593601595834 100 21.50385663510347 26.34197137664374 27.518448395259178 24.63572359299361 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2297.0 1 1698.0 2 2243.5 3 3208.5 4 2234.5 5 1601.5 6 2318.5 7 4226.0 8 6581.0 9 7598.0 10 6491.0 11 5096.5 12 4995.5 13 5494.0 14 5938.0 15 6512.0 16 7214.5 17 7777.5 18 8584.0 19 9306.5 20 9948.0 21 11065.0 22 12826.5 23 15185.0 24 18096.0 25 21926.5 26 27070.5 27 32953.0 28 39817.0 29 48684.5 30 59810.0 31 70813.5 32 83565.0 33 98098.0 34 111171.0 35 124321.0 36 140295.0 37 156565.5 38 166121.5 39 171463.0 40 174978.0 41 176750.0 42 180460.0 43 189276.5 44 200590.5 45 213240.5 46 224988.0 47 231820.5 48 238677.5 49 244033.5 50 243223.0 51 240697.0 52 236359.5 53 230019.5 54 221705.5 55 205892.5 56 186062.5 57 165661.5 58 145729.5 59 126299.5 60 104062.0 61 82616.0 62 65718.0 63 51054.5 64 37772.5 65 27130.0 66 19558.5 67 14747.0 68 11018.0 69 8078.0 70 6107.5 71 4484.5 72 3334.5 73 2520.5 74 1849.0 75 1237.5 76 764.0 77 437.5 78 250.0 79 138.0 80 88.0 81 63.5 82 45.5 83 34.0 84 17.5 85 7.0 86 7.0 87 5.0 88 4.0 89 2.0 90 0.5 91 0.5 92 1.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02819684410589099 2 0.001986859244102712 3 4.96714811025678E-5 4 0.020878579223446 5 5.795006128632911E-4 6 4.96714811025678E-5 7 0.0 8 0.0041227329315131275 9 0.009950853380881083 10-11 0.0010431011031539238 12-13 9.93429622051356E-5 14-15 1.241787027564195E-4 16-17 3.394217875342133E-4 18-19 0.0 20-21 8.278580183761299E-5 22-23 2.566359856966003E-4 24-25 1.65571603675226E-5 26-27 8.278580183761299E-5 28-29 9.272009805812656E-4 30-31 0.002955453125602784 32-33 0.010306832328782819 34-35 0.01947949917239034 36-37 0.02255085242056578 38-39 0.021673322921087083 40-41 0.0044869904595986245 42-43 0.004834690827316599 44-45 0.008932588018278443 46-47 0.011631405158184626 48-49 1.20616429703347 50-51 0.017476082767920103 52-53 0.017931404678026976 54-55 0.009056766721034861 56-57 0.0071858075995048076 58-59 0.008651116292030558 60-61 0.005240341256320903 62-63 0.007111300377850956 64-65 0.018552298191809074 66-67 0.02731931460641229 68-69 0.02792365095982687 70-71 0.025108933697348023 72-73 0.02214520199156148 74-75 0.009205781164342567 76-77 0.014156372114231824 78-79 0.0080219441980647 80-81 0.0026739813993549 82-83 0.013477528539163397 84-85 0.02299789575048889 86-87 0.022161759151929 88-89 0.030978447047634784 90-91 0.02013350700690748 92-93 0.013030485209240286 94-95 0.00662286414700904 96-97 0.004147568672064411 98-99 0.015870038212270413 100 0.03586280935605395 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6039683.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.89583143954166 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43880019428056 47.312430922659935 2 14.747975747531486 18.256764418873146 3 4.274323964864403 7.936885069417214 4 1.702462857970775 4.2150141635615785 5 0.8352090798461667 2.5847980211466512 6 0.49242899699709447 1.8287581316444836 7 0.3041999374568451 1.3180095636923599 8 0.21105876510298738 1.0450926198921862 9 0.1634974843709812 0.9107831460073814 >10 0.7622496713188714 8.261682085832918 >50 0.04369529517503894 1.8475548431460525 >100 0.022649002237029793 2.5270677096033576 >500 0.0010109326934865143 0.42326125596037834 >1k 3.369770418164239E-4 0.49407184835675827 >5k 0.0 0.0 >10k+ 1.0109311254431179E-4 1.037826200205538 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20869 0.3455313797098291 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17559 0.29072717889332933 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11330 0.18759262696403106 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.65571603675226E-5 5 0.0 0.0 0.0 0.0 1.65571603675226E-5 6 0.0 0.0 0.0 0.0 3.31143207350452E-5 7 0.0 0.0 0.0 0.0 3.31143207350452E-5 8 0.0 0.0 0.0 0.0 4.96714811025678E-5 9 0.0 0.0 0.0 0.0 8.2785801837613E-5 10-11 0.0 0.0 0.0 0.0 8.2785801837613E-5 12-13 0.0 0.0 0.0 0.0 8.2785801837613E-5 14-15 0.0 0.0 0.0 1.65571603675226E-5 9.93429622051356E-5 16-17 0.0 0.0 0.0 3.31143207350452E-5 9.93429622051356E-5 18-19 0.0 0.0 0.0 4.13929009188065E-5 1.324572829401808E-4 20-21 0.0 0.0 0.0 1.1590012257265821E-4 1.407358631239421E-4 22-23 0.0 0.0 0.0 3.228646271666907E-4 1.904073442265099E-4 24-25 0.0 0.0 0.0 8.858080796624591E-4 2.152430847777938E-4 26-27 0.0 0.0 0.0 0.0020199735648377573 2.48357405512839E-4 28-29 0.0 0.0 0.0 0.005546648723120071 2.566359856966003E-4 30-31 0.0 0.0 0.0 0.01872614837566806 2.814717262478842E-4 32-33 0.0 8.2785801837613E-6 0.0 0.05020958881451229 3.1458604698292943E-4 34-35 0.0 1.65571603675226E-5 0.0 0.09716569561680638 3.477003677179746E-4 36-37 4.96714811025678E-5 1.65571603675226E-5 0.0 0.171987503317641 3.477003677179746E-4 38-39 4.96714811025678E-5 1.65571603675226E-5 0.0 0.27724137177398217 3.559789479017359E-4 40-41 4.96714811025678E-5 1.65571603675226E-5 0.0 0.3931001014457216 3.973718488205424E-4 42-43 4.96714811025678E-5 1.65571603675226E-5 0.0 0.5071954935383198 3.973718488205424E-4 44-45 5.79500612863291E-5 1.65571603675226E-5 0.0 0.6478982423415269 4.056504290043037E-4 46-47 6.62286414700904E-5 1.65571603675226E-5 0.0 0.8121204374467997 4.13929009188065E-4 48-49 6.62286414700904E-5 1.65571603675226E-5 0.0 0.9833545899677185 4.636004902906328E-4 50-51 6.62286414700904E-5 1.65571603675226E-5 0.0 1.166882434061523 4.636004902906328E-4 52-53 6.62286414700904E-5 1.65571603675226E-5 0.0 1.3460391878183011 4.8015765065815544E-4 54-55 6.62286414700904E-5 1.65571603675226E-5 0.0 1.5533845071007866 4.96714811025678E-4 56-57 6.62286414700904E-5 1.65571603675226E-5 0.0 1.7877345549427015 5.049933912094393E-4 58-59 8.2785801837613E-5 1.65571603675226E-5 0.0 2.049743339178563 5.132719713932006E-4 60-61 8.2785801837613E-5 1.65571603675226E-5 0.0 2.3175802438637922 5.132719713932006E-4 62-63 8.2785801837613E-5 1.65571603675226E-5 0.0 2.5911376474560006 5.298291317607232E-4 64-65 8.2785801837613E-5 1.65571603675226E-5 0.0 2.869587691936812 5.298291317607232E-4 66-67 8.2785801837613E-5 1.65571603675226E-5 0.0 3.1809864855489933 5.546648723120071E-4 68-69 8.2785801837613E-5 1.65571603675226E-5 0.0 3.5239183910811214 5.629434524957684E-4 70-71 8.2785801837613E-5 1.65571603675226E-5 0.0 3.887414289789713 5.712220326795297E-4 72-73 1.324572829401808E-4 1.65571603675226E-5 0.0 4.23749888860061 6.62286414700904E-4 74-75 1.324572829401808E-4 1.65571603675226E-5 0.0 4.591424748616774 6.62286414700904E-4 76-77 1.324572829401808E-4 1.65571603675226E-5 0.0 4.982289633412879 6.62286414700904E-4 78-79 1.324572829401808E-4 1.65571603675226E-5 0.0 5.408446105532359 6.705649948846653E-4 80-81 1.324572829401808E-4 1.65571603675226E-5 0.0 5.849363286119487 7.036793156197105E-4 82-83 1.324572829401808E-4 1.65571603675226E-5 0.0 6.298525932569639 7.285150561709944E-4 84-85 1.324572829401808E-4 1.65571603675226E-5 0.0 6.743863874974895 7.285150561709944E-4 86-87 1.407358631239421E-4 1.65571603675226E-5 0.0 7.205858320709879 7.285150561709944E-4 88 1.490144433077034E-4 1.65571603675226E-5 0.0 7.5698674913898625 7.285150561709944E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10700 0.0 55.265106 1 GTATCAA 18470 0.0 38.69878 1 TACCTGG 5245 0.0 35.87108 2 ATCAACG 23050 0.0 30.353573 3 CAACGCA 23150 0.0 30.154154 5 TATCAAC 23905 0.0 29.53883 2 TCAACGC 23735 0.0 29.51783 4 ACCTGGG 6275 0.0 29.089682 3 GTACCTG 6645 0.0 28.95697 1 AACGCAG 24725 0.0 28.422817 6 GTGGTAT 4555 0.0 27.715847 1 GTACATG 22505 0.0 26.546888 1 TACATGG 22980 0.0 25.501043 2 ACGCAGA 27950 0.0 24.92506 7 TGGTATC 5000 0.0 24.491535 2 ACATGGG 23395 0.0 24.189526 3 CGCAGAG 28850 0.0 24.147503 8 CATGGGG 16110 0.0 22.458082 4 ATGGGGG 9915 0.0 22.427498 5 GCAGAGT 31335 0.0 21.88798 9 >>END_MODULE