FastQCFastQC Report
Mon 6 Jun 2016
ERR1211322_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211322_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250502
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108280.481137097412044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96440.42852661317341645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61370.2726947143348462No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27280.12121739949575694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.056.9353641
TACCTGG17650.053.8426062
ACCTGGG21950.043.4540523
GTACCTG24350.039.608981
GTATCAA75100.038.4651261
GTACATG90700.033.405451
ACATGGG95350.030.5520063
TACATGG98300.030.2949032
CAACGCA94500.029.7840025
CATGGGG54600.029.6889744
ATCAACG95100.029.5947763
TCAACGC96850.029.1085364
AACGCAG98900.028.6502486
CCTGGGG19950.028.2622724
TATCAAC100550.028.0261962
ATGGGGG36900.027.3779015
TAACGCA2450.026.8491614
CGTTAAC1807.4251147E-926.1376291
ACGCAGA109850.025.6665927
CGCAGAG111450.025.2132288
Produced by FastQC (version 0.11.5)