##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211322_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2250502 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.31951182447294 31.0 31.0 34.0 28.0 34.0 2 31.31993928465738 31.0 31.0 34.0 28.0 34.0 3 31.355450917173147 31.0 31.0 34.0 28.0 34.0 4 34.9888864795499 37.0 35.0 37.0 32.0 37.0 5 35.049139703052916 37.0 35.0 37.0 32.0 37.0 6 35.11360798612932 37.0 35.0 37.0 32.0 37.0 7 35.05107971465922 37.0 35.0 37.0 32.0 37.0 8 35.0485309499836 37.0 35.0 37.0 32.0 37.0 9 36.6009414788345 39.0 37.0 39.0 32.0 39.0 10-11 36.5231599438703 39.0 37.0 39.0 32.0 39.0 12-13 36.4518791807339 39.0 37.0 39.0 32.0 39.0 14-15 37.557904192042486 40.0 37.0 41.0 32.0 41.0 16-17 37.55489864039223 40.0 37.0 41.0 32.0 41.0 18-19 37.59712633003659 40.0 37.0 41.0 32.0 41.0 20-21 37.570375631747936 40.0 37.0 41.0 32.0 41.0 22-23 37.43765768704049 40.0 37.0 41.0 31.0 41.0 24-25 37.32999326372516 40.0 37.0 41.0 31.0 41.0 26-27 37.18741707405725 40.0 36.0 41.0 31.0 41.0 28-29 37.053398530638944 40.0 36.0 41.0 30.0 41.0 30-31 36.85591681322656 39.0 36.0 41.0 30.0 41.0 32-33 36.6962715429713 39.0 35.5 41.0 30.0 41.0 34-35 36.53506662069174 39.0 35.0 41.0 30.0 41.0 36-37 36.4256996883362 39.0 35.0 41.0 29.5 41.0 38-39 36.26871582429165 39.0 35.0 41.0 29.0 41.0 40-41 36.117533554735786 39.0 35.0 40.0 28.0 41.0 42-43 35.97637082748649 39.0 35.0 40.0 27.0 41.0 44-45 35.831419612157646 39.0 35.0 40.0 27.0 41.0 46-47 35.6531396106291 39.0 35.0 40.0 26.0 41.0 48-49 35.41056795328331 38.5 34.0 40.0 25.0 41.0 50-51 34.75427104708194 37.5 33.5 39.5 24.0 40.5 52-53 35.00472050235903 38.0 34.0 40.0 24.5 40.5 54-55 35.38874237836714 39.0 34.0 40.0 26.0 41.0 56-57 35.32049049500956 38.5 34.0 40.0 25.5 41.0 58-59 35.130768379677065 38.0 34.0 40.0 25.0 41.0 60-61 34.89309429629478 38.0 34.0 40.0 25.0 41.0 62-63 34.630262048200805 37.5 33.5 40.0 24.0 41.0 64-65 34.38303809550047 37.0 33.0 40.0 23.5 41.0 66-67 34.18047684472175 37.0 33.0 40.0 23.0 41.0 68-69 33.84782372999446 36.0 33.0 39.0 23.0 41.0 70-71 33.512333248315265 36.0 32.0 39.0 22.5 41.0 72-73 33.14110896146727 35.0 32.0 39.0 22.0 40.0 74-75 32.751981557892414 35.0 32.0 37.5 21.5 40.0 76-77 32.377160740137086 35.0 31.0 37.0 20.0 39.0 78-79 31.985934471509026 35.0 31.0 36.5 20.0 39.0 80-81 31.631562646911668 35.0 31.0 36.0 20.0 38.5 82-83 31.28746075320084 35.0 30.0 36.0 19.0 37.0 84-85 30.9421242460571 34.0 30.0 35.0 18.0 37.0 86-87 30.666690809428296 34.0 30.0 35.0 18.0 36.5 88-89 30.36988014229714 34.0 29.0 35.0 17.0 36.0 90-91 30.080735098213644 34.0 29.0 35.0 15.5 36.0 92-93 29.818744884474665 34.0 29.0 35.0 14.0 35.5 94-95 29.570261435004277 34.0 29.0 35.0 11.5 35.0 96-97 29.350289846443147 34.0 27.5 35.0 10.5 35.0 98-99 29.099372495558768 34.0 27.0 35.0 10.0 35.0 100 27.44339796187695 32.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5378434358762973 1101 2 -0.5641669318656959 1101 3 -0.5159739742894693 1101 4 -0.30400464443353314 1101 5 -0.47149074656871903 1101 6 -0.40266127158450615 1101 7 -0.44701558488641524 1101 8 -0.48593633068198017 1101 9 -0.7033100645834551 1101 10-11 -0.7277414308353016 1101 12-13 -0.7438383805021616 1101 14-15 -0.8337305554686836 1101 16-17 -0.7570164945251108 1101 18-19 -0.9364389816147778 1101 20-21 -0.8575961026197447 1101 22-23 -0.8126250219176896 1101 24-25 -0.7953722366650311 1101 26-27 -0.7751869897262651 1101 28-29 -0.9385038637666057 1101 30-31 -0.9587600833889454 1101 32-33 -1.0045100911190872 1101 34-35 -0.9837434932266618 1101 36-37 -0.988771660658017 1101 38-39 -0.9115570144953153 1101 40-41 -1.0719330606620616 1101 42-43 -1.0445463787905496 1101 44-45 -1.1537060214011703 1101 46-47 -1.0022757306671437 1101 48-49 -1.0084422959071446 1101 50-51 -0.8768852935491154 1101 52-53 -0.7716387894415959 1101 54-55 -0.543362252877607 1101 56-57 -0.589806035769314 1101 58-59 -0.8479250779975374 1101 60-61 -0.8659021811725651 1101 62-63 -0.8408847758807028 1101 64-65 -0.8999231504724037 1101 66-67 -0.9954952475450014 1101 68-69 -0.8500849968049238 1101 70-71 -0.7916190219936041 1101 72-73 -0.9577664948000759 1101 74-75 -1.1051567685670953 1101 76-77 -0.8902416608060761 1101 78-79 -1.0094592381521537 1101 80-81 -1.0337265400873576 1101 82-83 -1.1293598358975885 1101 84-85 -1.068876556788613 1101 86-87 -1.2226952653923817 1101 88-89 -1.2517860570353 1101 90-91 -1.138735908292123 1101 92-93 -0.9383499054459605 1101 94-95 -1.0994537297929305 1101 96-97 -1.028277939885129 1101 98-99 -1.362388448040118 1101 100 -1.2412460908469356 1102 1 -0.48477600421655254 1102 2 -0.43856236769351753 1102 3 -0.4060530716004571 1102 4 -0.4257898795669206 1102 5 -0.4824480676275016 1102 6 -0.3992345492800524 1102 7 -0.5108926477230398 1102 8 -0.5330369854316501 1102 9 -0.4186178698483971 1102 10-11 -0.4699511198199602 1102 12-13 -0.5363562895516125 1102 14-15 -0.8012794688251859 1102 16-17 -0.6658662096342383 1102 18-19 -0.7814269577032888 1102 20-21 -0.8347489534571935 1102 22-23 -0.7581257381739874 1102 24-25 -0.8418669401729701 1102 26-27 -0.7737264715382182 1102 28-29 -0.8824699526901796 1102 30-31 -0.8347575184991953 1102 32-33 -0.8809778088586171 1102 34-35 -0.7853015290093595 1102 36-37 -0.9280235625285229 1102 38-39 -0.9572207972902618 1102 40-41 -0.9910049499670421 1102 42-43 -0.671508025392697 1102 44-45 -0.8712434418956647 1102 46-47 -1.028607066444188 1102 48-49 -1.140170574001644 1102 50-51 -1.0231482111297154 1102 52-53 -0.8748448223751168 1102 54-55 -0.7854369337591365 1102 56-57 -0.7952336785595335 1102 58-59 -0.880370748464685 1102 60-61 -0.8436856862999704 1102 62-63 -0.7971074076774087 1102 64-65 -1.0328845994501776 1102 66-67 -0.8841822554325347 1102 68-69 -0.8519368007086996 1102 70-71 -0.9054227947171043 1102 72-73 -0.9823551428114925 1102 74-75 -1.0322932123532595 1102 76-77 -0.8372660399405341 1102 78-79 -0.848378190489278 1102 80-81 -0.7929361535097499 1102 82-83 -0.7828047354182708 1102 84-85 -0.7601591284842577 1102 86-87 -0.8560593608460181 1102 88-89 -0.8234999307427451 1102 90-91 -0.9734730440806878 1102 92-93 -0.9798109995981257 1102 94-95 -1.0720699384800554 1102 96-97 -1.1394649068210931 1102 98-99 -1.0122532549943841 1102 100 -1.0910898671847455 1103 1 -0.5259578313149618 1103 2 -0.5917387913385319 1103 3 -0.48141107267936434 1103 4 -0.3793138272978709 1103 5 -0.3715518834820912 1103 6 -0.592509434367507 1103 7 -0.5135720829462471 1103 8 -0.5887823900442015 1103 9 -0.6412277779629463 1103 10-11 -0.7428923907015061 1103 12-13 -0.880785482912323 1103 14-15 -1.0552038828937853 1103 16-17 -1.0844825443808475 1103 18-19 -1.0602509486797018 1103 20-21 -1.1070329044872835 1103 22-23 -1.0267098617400876 1103 24-25 -0.9737940158436089 1103 26-27 -0.9498569836625563 1103 28-29 -0.9103186916614732 1103 30-31 -0.9888612674793578 1103 32-33 -1.0770310475869422 1103 34-35 -1.198740111539088 1103 36-37 -1.3582635079589949 1103 38-39 -1.194147126196512 1103 40-41 -1.2155322035236509 1103 42-43 -1.0934031573805214 1103 44-45 -1.2625669313139056 1103 46-47 -1.3920160570416371 1103 48-49 -1.338524850175176 1103 50-51 -1.3900115443292904 1103 52-53 -1.2086005791483885 1103 54-55 -1.377083036540668 1103 56-57 -1.1342934130234923 1103 58-59 -1.0684305431156247 1103 60-61 -1.023169672104764 1103 62-63 -1.006540267985656 1103 64-65 -1.148170253548912 1103 66-67 -0.953468894814435 1103 68-69 -1.0609087847836705 1103 70-71 -1.0931186810484235 1103 72-73 -1.2303288477882894 1103 74-75 -1.1862016322889701 1103 76-77 -1.1383288417113704 1103 78-79 -1.1779225304610108 1103 80-81 -1.240891969578339 1103 82-83 -1.3293822138958546 1103 84-85 -1.1937255191824718 1103 86-87 -1.2472237448657069 1103 88-89 -1.2456483496084694 1103 90-91 -1.1066944184838299 1103 92-93 -1.0676218542086744 1103 94-95 -1.5089998340222088 1103 96-97 -1.5151698746990725 1103 98-99 -1.5480550347456976 1103 100 -1.4692190329040216 1104 1 -0.4225661664826177 1104 2 -0.33540120237382354 1104 3 -0.44788880398760256 1104 4 -0.27304227399241654 1104 5 -0.22954770122367307 1104 6 -0.36962074113982624 1104 7 -0.3776119978336183 1104 8 -0.3277397603575025 1104 9 -0.4374252245841177 1104 10-11 -0.37593590086365936 1104 12-13 -0.3392334614874173 1104 14-15 -0.550067776062761 1104 16-17 -0.5500660428033299 1104 18-19 -0.6911116134386717 1104 20-21 -0.6371295733551392 1104 22-23 -0.5483227827174133 1104 24-25 -0.5642903838823514 1104 26-27 -0.7252807124761134 1104 28-29 -0.5835522377763738 1104 30-31 -0.7506651415713677 1104 32-33 -0.798420181052343 1104 34-35 -0.8425190021400155 1104 36-37 -0.8358664135279952 1104 38-39 -0.8689169185510508 1104 40-41 -0.7951464452141579 1104 42-43 -0.7696633378779936 1104 44-45 -0.7845971609712521 1104 46-47 -0.7700702062822273 1104 48-49 -0.7541413664272909 1104 50-51 -0.7975020058451818 1104 52-53 -0.6335767329380957 1104 54-55 -0.7932131634758832 1104 56-57 -0.7504106996916278 1104 58-59 -0.8027654172810088 1104 60-61 -0.6124874870581678 1104 62-63 -0.8445144407055878 1104 64-65 -0.839164640517879 1104 66-67 -0.8554380414869698 1104 68-69 -0.6881283166642191 1104 70-71 -0.8323053418452488 1104 72-73 -0.7493874727791194 1104 74-75 -0.8413441592535307 1104 76-77 -0.7724591887654455 1104 78-79 -0.7334933720488017 1104 80-81 -0.724772848586337 1104 82-83 -0.8950785232362293 1104 84-85 -0.6566630054250417 1104 86-87 -0.8797490585350864 1104 88-89 -0.740709432593281 1104 90-91 -0.8605143979394043 1104 92-93 -0.9619418703507243 1104 94-95 -0.9005500211232409 1104 96-97 -0.795637994150244 1104 98-99 -0.8869005113030433 1104 100 -0.9263141976263078 1105 1 -0.47486805199380555 1105 2 -0.5137812078925421 1105 3 -0.3817417278817494 1105 4 -0.3957982678696297 1105 5 -0.3621968453283344 1105 6 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-0.6121522912568409 1105 68-69 -0.553516961839307 1105 70-71 -0.6204624727607353 1105 72-73 -0.4971568643696358 1105 74-75 -0.6037176284618511 1105 76-77 -0.6371960019315139 1105 78-79 -0.49141094551578135 1105 80-81 -0.5244915409492492 1105 82-83 -0.5867761725108274 1105 84-85 -0.5361245073017358 1105 86-87 -0.5366737189173065 1105 88-89 -0.603824203930106 1105 90-91 -0.6494383974944604 1105 92-93 -0.6275908175755163 1105 94-95 -0.7214839766679191 1105 96-97 -0.8265260896073379 1105 98-99 -0.7643250539880917 1105 100 -0.885411580694992 1106 1 -0.5880619663917379 1106 2 -0.6667252565912385 1106 3 -0.6095399255883827 1106 4 -0.3879826743277377 1106 5 -0.38653342124086265 1106 6 -0.44231233461007236 1106 7 -0.461334302813988 1106 8 -0.4973810784425936 1106 9 -0.6545613544987674 1106 10-11 -0.6648703882779401 1106 12-13 -0.4942160496587178 1106 14-15 -0.6196514854459281 1106 16-17 -0.8450448021035868 1106 18-19 -0.8267501228486296 1106 20-21 -0.8185967335095796 1106 22-23 -0.6432193191447553 1106 24-25 -0.846863199550306 1106 26-27 -0.8793435937793532 1106 28-29 -0.8586064403888898 1106 30-31 -0.8206954689398387 1106 32-33 -0.7295201986410547 1106 34-35 -1.0336898162066674 1106 36-37 -1.094838730489272 1106 38-39 -0.9858086380277769 1106 40-41 -0.8273182456040189 1106 42-43 -0.8716918366218138 1106 44-45 -0.911445450612554 1106 46-47 -0.9881592885048534 1106 48-49 -0.9855168318948131 1106 50-51 -0.9481621850092452 1106 52-53 -0.7799604194383676 1106 54-55 -0.7917616550236346 1106 56-57 -0.5819082574690597 1106 58-59 -0.6319344142648262 1106 60-61 -0.6841835991482412 1106 62-63 -0.678314715651652 1106 64-65 -0.82568969917412 1106 66-67 -0.8588941872389952 1106 68-69 -0.8587089125178409 1106 70-71 -0.8682692551120397 1106 72-73 -0.6868743784666975 1106 74-75 -0.6632976412303648 1106 76-77 -0.6060702783402014 1106 78-79 -0.7527281799491234 1106 80-81 -0.8139459002592844 1106 82-83 -0.7430091137831312 1106 84-85 -0.85046397287989 1106 86-87 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0.5883177332158596 2214 84-85 0.6832749313473911 2214 86-87 0.6574830679293413 2214 88-89 0.5878576052210143 2214 90-91 0.47189978143389766 2214 92-93 0.45282461109073324 2214 94-95 0.5905611442152399 2214 96-97 0.7906639973112668 2214 98-99 0.6596074008579116 2214 100 0.9828876839085758 2215 1 0.3594428385747612 2215 2 0.2002786194744992 2215 3 0.30530251786634466 2215 4 0.15011313260354342 2215 5 0.2666626392214866 2215 6 0.3126743113271928 2215 7 0.43968730972920866 2215 8 0.4472936993165604 2215 9 0.5446595099316411 2215 10-11 0.42789630964924186 2215 12-13 0.4725430316388213 2215 14-15 0.5957361746156167 2215 16-17 0.5340180243934398 2215 18-19 0.5399228434649004 2215 20-21 0.5456099210570073 2215 22-23 0.5719918319433575 2215 24-25 0.5219915246499554 2215 26-27 0.5673294795093113 2215 28-29 0.6029495486140632 2215 30-31 0.6830768309186013 2215 32-33 0.733203334818981 2215 34-35 0.6650538499738516 2215 36-37 0.5920943458660162 2215 38-39 0.6244774669718822 2215 40-41 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2311 16-17 0.7764292907928549 2311 18-19 0.7226054590319748 2311 20-21 0.7658254622199934 2311 22-23 0.861453105808252 2311 24-25 0.9355602507568932 2311 26-27 1.0191879621288251 2311 28-29 0.9776860734177859 2311 30-31 1.0116631502102464 2311 32-33 1.0574294600644265 2311 34-35 0.9823039746333322 2311 36-37 0.9399077341528113 2311 38-39 0.7567156046015739 2311 40-41 0.8282844503615152 2311 42-43 0.9169252187332617 2311 44-45 0.9805126765862369 2311 46-47 0.8964374809294569 2311 48-49 1.056291392696835 2311 50-51 1.1894895299855577 2311 52-53 0.9771105320741142 2311 54-55 0.9254031126504216 2311 56-57 0.9323194334764651 2311 58-59 0.8586743537885795 2311 60-61 0.943094565073217 2311 62-63 0.8522813105387357 2311 64-65 0.9857276172024925 2311 66-67 0.9391118375241092 2311 68-69 1.0143227024991504 2311 70-71 0.9694465839965858 2311 72-73 0.9099757530527839 2311 74-75 0.8635999828590357 2311 76-77 0.9221806266280907 2311 78-79 0.8272529353956628 2311 80-81 0.8583843089482315 2311 82-83 0.8950420335407472 2311 84-85 0.9517290680417041 2311 86-87 0.9035413285402392 2311 88-89 1.0362315886058973 2311 90-91 0.9213462642992276 2311 92-93 0.8529165437756916 2311 94-95 1.057538871386626 2311 96-97 0.9773408308567859 2311 98-99 1.0139993877390587 2311 100 1.1495917523482895 2312 1 0.3236508932937632 2312 2 0.32835500375526294 2312 3 0.33559136932307965 2312 4 0.2852854623014167 2312 5 0.29765391072370306 2312 6 0.37022227836420996 2312 7 0.3545597851856215 2312 8 0.42850587724407063 2312 9 0.5860033242681624 2312 10-11 0.5741516763425594 2312 12-13 0.5881136734868377 2312 14-15 0.7833742056843036 2312 16-17 0.7104794380811299 2312 18-19 0.7216338332050825 2312 20-21 0.7365748766205016 2312 22-23 0.8409710684408438 2312 24-25 0.8206476535980656 2312 26-27 0.822481788676221 2312 28-29 0.7740524927097141 2312 30-31 0.7619018582837711 2312 32-33 0.7741108660549756 2312 34-35 0.6764055761065961 2312 36-37 0.7846213849258987 2312 38-39 0.8657998712504877 2312 40-41 0.8263356508442143 2312 42-43 0.8348630208856562 2312 44-45 0.846518427771457 2312 46-47 0.8836804686548874 2312 48-49 0.9107605866102659 2312 50-51 0.8359083690906175 2312 52-53 0.6718800513322449 2312 54-55 0.5159475439899168 2312 56-57 0.4795025132724362 2312 58-59 0.5842501095650263 2312 60-61 0.6472085607734712 2312 62-63 0.6298511184057318 2312 64-65 0.6237730808944235 2312 66-67 0.7066002027546645 2312 68-69 0.7049781061032263 2312 70-71 0.6595591966294592 2312 72-73 0.7779129104534732 2312 74-75 0.6954514368640972 2312 76-77 0.6703555294729995 2312 78-79 0.6724631949247168 2312 80-81 0.6689690027826316 2312 82-83 0.6907504312367934 2312 84-85 0.8038186033616519 2312 86-87 0.8397240295820687 2312 88-89 0.9070392405791203 2312 90-91 0.9766874773980625 2312 92-93 0.9002228011359819 2312 94-95 0.9006530131306398 2312 96-97 0.9143728048436053 2312 98-99 0.9383335766429077 2312 100 1.0128821991972075 2313 1 0.4918907898801166 2313 2 0.5730403271807454 2313 3 0.4950137492179003 2313 4 0.4515780498538504 2313 5 0.43677463252159043 2313 6 0.4419436453611638 2313 7 0.5588961312542509 2313 8 0.5455104824376491 2313 9 0.5924063700412816 2313 10-11 0.6666842531271087 2313 12-13 0.7070749833930279 2313 14-15 0.8364517056121983 2313 16-17 0.790123328748642 2313 18-19 0.7593965953772965 2313 20-21 0.9076264846799802 2313 22-23 0.7480690011293305 2313 24-25 0.7865640643963658 2313 26-27 0.8368713798374614 2313 28-29 0.7356232928631954 2313 30-31 0.8624825986282829 2313 32-33 0.8992084281604136 2313 34-35 0.8484251575750008 2313 36-37 0.9215623092683956 2313 38-39 0.9344213197932021 2313 40-41 0.9367392454997727 2313 42-43 0.7421713730758981 2313 44-45 0.8764298986278121 2313 46-47 0.8942777016930634 2313 48-49 0.8717663350175826 2313 50-51 0.8980398072063096 2313 52-53 0.7194936363474014 2313 54-55 0.6636270735592049 2313 56-57 0.7871432539840413 2313 58-59 0.7912262043363967 2313 60-61 0.8211885615251262 2313 62-63 0.8565421876164905 2313 64-65 0.8304638092386938 2313 66-67 0.7305244412458265 2313 68-69 0.7120272686642011 2313 70-71 0.7448035673069029 2313 72-73 0.7914610421118411 2313 74-75 0.6815357812161622 2313 76-77 0.7738873869418654 2313 78-79 0.7240655732987449 2313 80-81 0.7963114765818062 2313 82-83 0.957137368883572 2313 84-85 0.8067729980483271 2313 86-87 0.9135480620675196 2313 88-89 0.73424265833372 2313 90-91 0.88307745662269 2313 92-93 0.8573555633868537 2313 94-95 0.8171859552541321 2313 96-97 0.8479722325375185 2313 98-99 0.9444245984819624 2313 100 1.0696312244501698 2314 1 0.4845205507504531 2314 2 0.4108178575491763 2314 3 0.5017475491311352 2314 4 0.41476481874322957 2314 5 0.28478168624738487 2314 6 0.41057725899222675 2314 7 0.4550315545993726 2314 8 0.46855721763670743 2314 9 0.5757352457406952 2314 10-11 0.6717328967906795 2314 12-13 0.6998751581152121 2314 14-15 0.8275313189540512 2314 16-17 0.8479170857576932 2314 18-19 0.764956267312499 2314 20-21 0.7476650378813119 2314 22-23 0.7876092804374153 2314 24-25 0.8078775264934634 2314 26-27 0.915511720443348 2314 28-29 0.8458189893908781 2314 30-31 0.8296991538967617 2314 32-33 0.7786044763506368 2314 34-35 0.8518101866647996 2314 36-37 0.9059793595261922 2314 38-39 0.9597089011663442 2314 40-41 1.0373510290950776 2314 42-43 0.9261155302269017 2314 44-45 1.0137937008487725 2314 46-47 0.9579708919214198 2314 48-49 0.7235922373258745 2314 50-51 0.7748455221915904 2314 52-53 0.845711434207999 2314 54-55 0.8531658812082554 2314 56-57 0.8352468826686774 2314 58-59 0.924924509113147 2314 60-61 0.6488673115789823 2314 62-63 0.7273872568975008 2314 64-65 0.9039806435251947 2314 66-67 0.8433994103729816 2314 68-69 0.8729329233754441 2314 70-71 0.9076206725066385 2314 72-73 0.8842506379516024 2314 74-75 0.8304732220565327 2314 76-77 0.9433075490120473 2314 78-79 0.8306597575581378 2314 80-81 0.7877387427127829 2314 82-83 0.8199665886099439 2314 84-85 0.952949051980152 2314 86-87 0.9919823704069621 2314 88-89 0.7251693471051333 2314 90-91 0.8254792809680218 2314 92-93 0.9873774932394781 2314 94-95 0.8632542154929332 2314 96-97 0.9941751602432269 2314 98-99 0.9650273985585756 2314 100 0.8568562001677975 2315 1 0.26527688949528283 2315 2 0.3376875797912575 2315 3 0.3131825641138306 2315 4 0.21987561795000943 2315 5 0.27846875923425074 2315 6 0.18302548642651573 2315 7 0.2851745581377685 2315 8 0.313267324298792 2315 9 0.25787169845185787 2315 10-11 0.2876457040593081 2315 12-13 0.26954271241312 2315 14-15 0.44227364876235953 2315 16-17 0.2995963728430482 2315 18-19 0.41221190636309046 2315 20-21 0.2613468093306892 2315 22-23 0.1604608093749036 2315 24-25 0.37194940831767553 2315 26-27 0.40023990584999325 2315 28-29 0.30789271839117305 2315 30-31 0.4463892133885423 2315 32-33 0.4588404449411172 2315 34-35 0.3438432468518826 2315 36-37 0.44021943137289554 2315 38-39 0.5526756256565619 2315 40-41 0.548329141856442 2315 42-43 0.48920947071171383 2315 44-45 0.6212623044998935 2315 46-47 0.5944886179966673 2315 48-49 0.5555675743058899 2315 50-51 0.401367257868138 2315 52-53 0.23022300079978208 2315 54-55 0.3647425074486037 2315 56-57 0.28217130105698374 2315 58-59 0.30665715859831977 2315 60-61 0.33630801944294575 2315 62-63 0.35475857649951337 2315 64-65 0.4594770376016868 2315 66-67 0.2790829386883402 2315 68-69 0.30197406004515415 2315 70-71 0.26772614299927255 2315 72-73 0.33345475926984847 2315 74-75 0.21356574935547457 2315 76-77 0.3762138326300928 2315 78-79 0.40971057329667815 2315 80-81 0.5308911673508874 2315 82-83 0.3711009211242171 2315 84-85 0.41043368985545925 2315 86-87 0.3291319327165425 2315 88-89 0.27741332319135026 2315 90-91 0.44004015436705757 2315 92-93 0.5323786827405961 2315 94-95 0.5526995149566325 2315 96-97 0.5390906387676146 2315 98-99 0.6379221174662923 2315 100 0.5858230400542581 2316 1 0.18270058884536056 2316 2 0.2259315294686708 2316 3 0.2662306075668788 2316 4 0.20051556384330382 2316 5 0.19733483816128228 2316 6 0.01223024926952121 2316 7 0.09353538499772185 2316 8 0.13327660292733867 2316 9 0.2737677778120826 2316 10-11 0.25516449537820307 2316 12-13 0.18410946054725486 2316 14-15 0.09032555598166425 2316 16-17 0.34249822200106195 2316 18-19 0.4117026996998092 2316 20-21 0.3515076202604632 2316 22-23 0.23610329151983223 2316 24-25 0.19910567635266574 2316 26-27 0.18705971180927605 2316 28-29 0.17502359596218042 2316 30-31 -0.4066764371006073 2316 32-33 -0.19311358672080559 2316 34-35 -0.2264319539172419 2316 36-37 -0.048113391915343584 2316 38-39 0.02013467881723585 2316 40-41 0.33123976658482235 2316 42-43 0.23590074530741134 2316 44-45 0.17224040330331292 2316 46-47 -0.10188223648956551 2316 48-49 0.054888026555190095 2316 50-51 0.23321796697055674 2316 52-53 -0.11212303894460973 2316 54-55 0.005700735454126971 2316 56-57 0.056933151919800196 2316 58-59 -0.2697901467565984 2316 60-61 0.17995013267854176 2316 62-63 0.11122510985576639 2316 64-65 -0.18217289539376225 2316 66-67 -0.5255959914187187 2316 68-69 -0.4253693875522515 2316 70-71 -0.18938018253805922 2316 72-73 -0.5706379683255491 2316 74-75 -0.3696977147869589 2316 76-77 0.05023283425525449 2316 78-79 0.13206668817839784 2316 80-81 -0.08873837222512293 2316 82-83 0.16698380918119682 2316 84-85 0.15625020218108077 2316 86-87 0.3482598115018831 2316 88-89 0.27747746310859966 2316 90-91 -0.3430796587188816 2316 92-93 -0.2938154166664937 2316 94-95 0.1341356901494386 2316 96-97 0.3144154955707066 2316 98-99 0.4202572296692253 2316 100 0.33833532655147636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 61.0 1 176.0 2 104.0 3 81.0 4 86.0 5 76.0 6 106.0 7 278.0 8 744.0 9 1294.0 10 1750.0 11 2789.0 12 4018.0 13 5315.0 14 6248.0 15 7430.0 16 9305.0 17 11876.0 18 14637.0 19 17264.0 20 20116.0 21 24230.0 22 26492.0 23 26122.0 24 25609.0 25 26006.0 26 28389.0 27 31835.0 28 37439.0 29 43884.0 30 52407.0 31 64258.0 32 79913.0 33 102418.0 34 139249.0 35 190860.0 36 278314.0 37 417927.0 38 438291.0 39 112980.0 40 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45399541405305 19.99691544176963 12.605264994219786 22.943824149957532 2 15.957090923326318 21.091179563649177 40.38994339646143 22.561786116563074 3 17.617496791259427 28.155409705506944 30.955369174047064 23.271724329186565 4 11.737616878190693 17.17315549226945 39.78109407630509 31.308133553234768 5 11.699094151852895 38.55867835590917 35.92735437408835 13.814873118149585 6 28.243672117038436 38.99088176704606 19.14491059810647 13.62053551780903 7 25.879061670350538 33.384338529876715 24.191578007721017 16.545021792051724 8 25.439443813469474 35.631144134192446 21.634242455897812 17.295169596440264 9 26.902132482859013 15.312804898376466 22.122081587749616 35.6629810310149 10-11 23.894352689634633 26.81124439080659 29.849065745636555 19.445337173922223 12-13 24.144701427286787 25.415608540517137 29.47474566077524 20.964944371420838 14-15 22.094427266255945 26.538141764244532 27.20633909603342 24.161091873466102 16-17 20.580404620744453 29.111890248984558 28.598318617999567 21.709386512271422 18-19 20.373849099260287 28.32434897679684 30.76348286912059 20.53831905482228 20-21 21.198692816849878 27.83701287933718 30.673481173121747 20.290813130691195 22-23 21.2871124270088 27.576369856162604 30.235861323944224 20.900656392884372 24-25 20.823150993867475 27.724909648411376 30.746774555000638 20.705164802720507 26-27 20.55747649486104 27.81269159683779 30.71538230002014 20.91444960828103 28-29 20.468430708003137 27.66198318945176 31.044298570950023 20.825287531595077 30-31 21.17960660811586 27.692740973034635 30.75004262275186 20.377609796097644 32-33 20.9283091320132 28.08188136155681 30.491462874947462 20.498346631482526 34-35 21.160380577267386 27.92675187904747 30.5998392960143 20.313028247670847 36-37 21.23480338922927 27.670422746829765 30.22548038604248 20.869293477898484 38-39 20.858089109681146 28.26897387168117 30.40233832338657 20.470598695251113 40-41 20.75214046843559 27.611086955168645 31.036903463072974 20.59986911332279 42-43 20.71708604018801 27.865961120882364 30.19909497836591 21.21785786056371 44-45 20.358936395830415 28.35277405414057 30.329858679631595 20.958430870397418 46-47 20.563332312175504 27.902707817103963 29.99445106690244 21.53950880381809 48-49 20.67314596745114 28.06822340026793 29.986203437713833 21.272427194567097 50-51 20.733328442846886 28.012083947422823 29.809960142535452 21.44462746719484 52-53 20.63702593767577 28.495294669515282 29.539159954558492 21.328519438250456 54-55 20.811010930595508 28.179165470939438 29.6167056079181 21.393117990546955 56-57 20.841966876094684 28.410763457489622 29.261413337903804 21.485856328511886 58-59 20.5116360853244 28.132271094524036 29.802765291652932 21.553327528498635 60-61 21.048630512885545 28.699758002187693 29.261307623964694 20.990303860962065 62-63 20.880998904086447 28.38173803442458 29.612051047786718 21.125212013702253 64-65 20.714490540369315 27.97407632169191 29.508468208927685 21.80296492901109 66-67 20.715012914956898 28.06995860989717 29.121227209880274 22.093801265265654 68-69 20.40427114995286 28.123205598831753 29.400291261251027 22.072231989964358 70-71 20.651294125179202 27.767832193327045 29.66285320873108 21.918020472762674 72-73 20.69775879340234 28.15153699189143 29.049718087360237 22.100986127345994 74-75 20.665643552647843 28.259708839296117 29.052540698568734 22.022106909487306 76-77 20.55491166040036 27.87757782202797 29.388115844222128 22.17939467334954 78-79 20.40506638655929 27.853743935175153 29.291361688647317 22.449827989618242 80-81 20.39647832701192 28.145795109736042 28.908899842892815 22.54882672035922 82-83 21.003655330193418 27.781732062785252 29.169752787289305 22.044859819732025 84-85 20.609113426318043 28.05533732855472 28.763993389611446 22.571555855515793 86-87 20.311236023244444 28.24581172496933 29.241687860999267 22.201264390786964 88-89 20.709013498783598 27.602732614972204 29.220123389628842 22.468130496615355 90-91 20.926222364512746 27.624682069615638 29.102880493748113 22.346215072123506 92-93 20.982511201888794 27.658865828352802 28.93785883785837 22.420764131900032 94-95 20.62086909577806 27.154495984659928 29.16325551383857 23.061379405723446 96-97 20.926628633733696 27.17229167653286 28.97993432615608 22.921145363577363 98-99 20.636401255254036 27.321009228514182 28.849007294891088 23.193582221340698 100 20.983769349559385 26.972884256122253 28.601110220802873 23.44223617351549 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 958.0 1 706.5 2 1028.5 3 1465.5 4 1037.0 5 861.5 6 1288.0 7 2354.0 8 3753.5 9 4349.5 10 3626.0 11 2822.5 12 2764.5 13 2898.5 14 3135.0 15 3425.5 16 3579.0 17 3716.5 18 3944.0 19 4268.0 20 4682.0 21 5285.0 22 6139.0 23 7281.5 24 8504.0 25 10065.5 26 13019.5 27 16617.5 28 18810.5 29 22287.0 30 29564.5 31 36107.0 32 43643.5 33 54517.5 34 65813.5 35 74440.0 36 83318.0 37 91800.0 38 93511.0 39 92626.5 40 87828.5 41 81653.5 42 77263.5 43 76078.5 44 77653.0 45 79946.5 46 79580.5 47 75644.5 48 74057.0 49 73408.5 50 72073.5 51 68809.0 52 64378.5 53 60584.5 54 57219.0 55 52277.0 56 46921.5 57 41204.5 58 35357.0 59 30348.0 60 24803.5 61 19529.0 62 15407.0 63 12244.5 64 9302.5 65 6700.5 66 4877.0 67 3704.0 68 2864.0 69 2146.5 70 1744.0 71 1395.0 72 1033.5 73 776.0 74 607.0 75 475.5 76 353.0 77 226.5 78 138.0 79 92.0 80 64.0 81 48.0 82 35.5 83 23.0 84 20.0 85 17.0 86 14.0 87 12.5 88 7.5 89 3.5 90 2.5 91 1.5 92 2.5 93 4.0 94 2.0 95 0.5 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025994200405065185 2 0.11490769614957018 3 0.017684943181565712 4 0.017907115834600456 5 0.020262145956768757 6 0.04678956072911732 7 0.047011733382152074 8 0.03985777395443328 9 0.0345256302815994 10-11 0.03763604742408582 12-13 0.03772491648529973 14-15 0.03188177571048593 16-17 0.03841365170970743 18-19 0.03781378554651362 20-21 0.048211465708539694 22-23 0.048300334769753595 24-25 0.06709614121649303 26-27 0.058342538686924066 28-29 0.05067758215722537 30-31 0.04961115342265859 32-33 0.04459005146407335 34-35 0.045523176606819274 36-37 0.04021325019928887 38-39 0.04563426293333665 40-41 0.054565603585333404 42-43 0.056609591993253064 44-45 0.06029765803362983 46-47 0.06314146799247457 48-49 0.06180843207426609 50-51 0.05496551436079594 52-53 0.06474111109432473 54-55 0.052143921667254685 56-57 0.05689841644219824 58-59 0.05454338632002993 60-61 0.048944635463554355 62-63 0.05496551436079594 64-65 0.05363247844258748 66-67 0.05207726987134426 68-69 0.058586928605262295 70-71 0.058586928605262295 72-73 0.06225277738033559 74-75 0.05154405550406087 76-77 0.05203283534073731 78-79 0.060630917013181955 80-81 0.06223056011503211 82-83 0.05261048423862765 84-85 0.058431407748137966 86-87 0.05672067831977043 88-89 0.06431898305355872 90-91 0.07029542742019336 92-93 0.07387240713405276 94-95 0.07780486309276774 96-97 0.07382797260344581 98-99 0.083248093092119 100 0.12623850145434218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2250502.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.018637216139474 #Duplication Level Percentage of deduplicated Percentage of total 1 79.38405933027174 49.23291176348615 2 14.01646772954092 17.385644543402492 3 3.499707578178244 6.511412839608317 4 1.2275773637819536 3.045307007165514 5 0.5529427744923565 1.7146378666263538 6 0.32430334145516826 1.206771076901393 7 0.1904400159326287 0.8267581181693064 8 0.13014528134675316 0.6457146391389349 9 0.09520040868150934 0.5313779647962069 >10 0.46410931670157185 5.262994706282183 >50 0.05125689209513289 2.2562243143183096 >100 0.05853550540247945 7.362014089517189 >500 0.0037418139335781207 1.580096713289749 >1k 0.001273808998664887 1.1243166757802876 >5k 1.5922612483311089E-4 0.7791858706463799 >10k+ 7.961306241655544E-5 0.5346318108712377 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10828 0.481137097412044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9644 0.42852661317341645 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6137 0.2726947143348462 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2728 0.12121739949575694 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2217265303474513E-5 0.0 14-15 0.0 0.0 0.0 4.4434530606949026E-5 6.665179591042354E-5 16-17 0.0 0.0 0.0 4.4434530606949026E-5 8.886906121389805E-5 18-19 0.0 0.0 0.0 4.4434530606949026E-5 2.2217265303474515E-4 20-21 0.0 0.0 0.0 4.4434530606949026E-5 2.2217265303474515E-4 22-23 0.0 0.0 0.0 4.4434530606949026E-5 2.2217265303474515E-4 24-25 2.2217265303474513E-5 0.0 0.0 1.9995538773127063E-4 2.2217265303474515E-4 26-27 4.4434530606949026E-5 0.0 0.0 0.0011330805304772002 2.2217265303474515E-4 28-29 4.4434530606949026E-5 0.0 0.0 0.007553870203181335 2.2217265303474515E-4 30-31 4.4434530606949026E-5 0.0 0.0 0.0285936204455717 2.2217265303474515E-4 32-33 4.4434530606949026E-5 0.0 0.0 0.071917287787347 2.2217265303474515E-4 34-35 4.4434530606949026E-5 0.0 0.0 0.14063528937099368 2.2217265303474515E-4 36-37 4.4434530606949026E-5 0.0 0.0 0.2501886245824265 2.2217265303474515E-4 38-39 4.4434530606949026E-5 0.0 0.0 0.41230800950188 3.110417142486432E-4 40-41 4.4434530606949026E-5 0.0 0.0 0.5863580658892993 3.110417142486432E-4 42-43 4.4434530606949026E-5 0.0 0.0 0.753209728318393 3.110417142486432E-4 44-45 4.4434530606949026E-5 0.0 0.0 0.9602302064161685 3.110417142486432E-4 46-47 4.4434530606949026E-5 0.0 0.0 1.2141957661001856 3.110417142486432E-4 48-49 4.4434530606949026E-5 2.2217265303474513E-5 0.0 1.4747376363140314 3.110417142486432E-4 50-51 4.4434530606949026E-5 4.4434530606949026E-5 0.0 1.739589655996751 3.110417142486432E-4 52-53 4.4434530606949026E-5 4.4434530606949026E-5 0.0 1.9961546357212747 3.5547624485559226E-4 54-55 4.4434530606949026E-5 4.4434530606949026E-5 0.0 2.287822894625288 3.9991077546254125E-4 56-57 4.4434530606949026E-5 4.4434530606949026E-5 0.0 2.634723275073739 3.9991077546254125E-4 58-59 4.4434530606949026E-5 4.4434530606949026E-5 0.0 3.0109948802533832 3.9991077546254125E-4 60-61 4.4434530606949026E-5 4.4434530606949026E-5 0.0 3.3835562021273473 3.9991077546254125E-4 62-63 4.4434530606949026E-5 4.4434530606949026E-5 0.0 3.7520517644507763 3.9991077546254125E-4 64-65 4.4434530606949026E-5 4.4434530606949026E-5 0.0 4.121702624569985 3.9991077546254125E-4 66-67 4.4434530606949026E-5 4.4434530606949026E-5 0.0 4.540853551785335 3.9991077546254125E-4 68-69 4.4434530606949026E-5 4.4434530606949026E-5 0.0 4.9998178184245115 3.9991077546254125E-4 70-71 4.4434530606949026E-5 4.4434530606949026E-5 0.0 5.4855538897543745 4.443453060694903E-4 72-73 4.4434530606949026E-5 4.4434530606949026E-5 0.0 5.950094689984724 4.887798366764393E-4 74-75 4.4434530606949026E-5 4.4434530606949026E-5 0.0 6.401482869155415 4.887798366764393E-4 76-77 4.4434530606949026E-5 8.886906121389805E-5 0.0 6.915745909134939 4.887798366764393E-4 78-79 4.4434530606949026E-5 8.886906121389805E-5 0.0 7.469000249722062 4.887798366764393E-4 80-81 4.4434530606949026E-5 8.886906121389805E-5 0.0 8.035673818552482 4.887798366764393E-4 82-83 8.886906121389805E-5 8.886906121389805E-5 0.0 8.585240093099229 4.887798366764393E-4 84-85 1.3330359182084708E-4 8.886906121389805E-5 0.0 9.126363806830653 4.887798366764393E-4 86-87 1.3330359182084708E-4 8.886906121389805E-5 0.0 9.684794770233484 4.887798366764393E-4 88 1.3330359182084708E-4 8.886906121389805E-5 0.0 10.129517769813136 4.887798366764393E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4330 0.0 56.935364 1 TACCTGG 1765 0.0 53.842606 2 ACCTGGG 2195 0.0 43.454052 3 GTACCTG 2435 0.0 39.60898 1 GTATCAA 7510 0.0 38.465126 1 GTACATG 9070 0.0 33.40545 1 ACATGGG 9535 0.0 30.552006 3 TACATGG 9830 0.0 30.294903 2 CAACGCA 9450 0.0 29.784002 5 CATGGGG 5460 0.0 29.688974 4 ATCAACG 9510 0.0 29.594776 3 TCAACGC 9685 0.0 29.108536 4 AACGCAG 9890 0.0 28.650248 6 CCTGGGG 1995 0.0 28.262272 4 TATCAAC 10055 0.0 28.026196 2 ATGGGGG 3690 0.0 27.377901 5 TAACGCA 245 0.0 26.849161 4 CGTTAAC 180 7.4251147E-9 26.137629 1 ACGCAGA 10985 0.0 25.666592 7 CGCAGAG 11145 0.0 25.213228 8 >>END_MODULE