FastQCFastQC Report
Mon 6 Jun 2016
ERR1211322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211322_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250502
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92620.4115526224815619No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88920.39511184615699074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55750.2477225081337408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43950.057.3391271
TACCTGG19400.052.717562
ACCTGGG22450.044.3056033
GTACCTG27000.038.5855671
GTATCAA76600.035.10441
TAACGCA2150.032.7334634
CCTGGGG18550.032.374664
GTACATG89500.030.6214331
TACATGG95450.028.261322
ACATGGG92200.028.1406023
ATCAACG94750.027.5813243
CAACGCA95100.027.1765565
TCAACGC98650.026.3482534
TGGTATC21900.026.1346242
AACGCAG99900.026.1055366
TATCAAC103100.025.3907812
CATGGGG49800.025.2490234
ATGGGGG34150.023.7617655
ACGCAGA110900.023.4738737
GTGGTAT24800.023.4641951
Produced by FastQC (version 0.11.5)