##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211322_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2250502 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12827138122961 33.0 31.0 34.0 30.0 34.0 2 32.253669625710174 34.0 31.0 34.0 30.0 34.0 3 32.316419625488 34.0 31.0 34.0 30.0 34.0 4 35.95974453699664 37.0 35.0 37.0 35.0 37.0 5 35.87987613430248 37.0 35.0 37.0 35.0 37.0 6 35.89577392066303 37.0 35.0 37.0 35.0 37.0 7 35.81697239104875 37.0 35.0 37.0 35.0 37.0 8 35.8006644739707 37.0 35.0 37.0 33.0 37.0 9 37.42113492900695 39.0 37.0 39.0 34.0 39.0 10-11 37.37305521168166 39.0 37.0 39.0 34.0 39.0 12-13 37.313964839844616 39.0 37.0 39.0 34.0 39.0 14-15 38.54846985250401 40.0 38.0 41.0 34.0 41.0 16-17 38.53563849310065 40.0 38.0 41.0 34.0 41.0 18-19 38.57083530696707 40.0 38.0 41.0 34.0 41.0 20-21 38.546420309779776 40.0 38.0 41.0 34.0 41.0 22-23 38.43251105753294 40.0 38.0 41.0 34.0 41.0 24-25 38.35224740968904 40.0 38.0 41.0 34.0 41.0 26-27 38.24818995939573 40.0 38.0 41.0 34.0 41.0 28-29 38.151207152893 40.0 38.0 41.0 33.0 41.0 30-31 38.12572039482747 40.0 37.0 41.0 33.5 41.0 32-33 38.02669582164335 40.0 37.0 41.0 33.0 41.0 34-35 37.87911919207359 40.0 37.0 41.0 33.0 41.0 36-37 37.72201513262374 40.0 37.0 41.0 33.0 41.0 38-39 37.60788015296143 40.0 37.0 41.0 33.0 41.0 40-41 37.50527615616427 40.0 36.5 41.0 32.5 41.0 42-43 37.398941880522656 40.0 36.0 41.0 32.0 41.0 44-45 37.245428797663806 40.0 36.0 41.0 31.5 41.0 46-47 36.996855368268946 40.0 36.0 41.0 31.0 41.0 48-49 35.42237665196476 39.0 35.0 41.0 24.0 41.0 50-51 36.19672988515451 39.0 35.0 40.5 29.0 41.0 52-53 36.32348404933654 39.0 35.0 40.0 29.5 41.0 54-55 36.17827733545671 39.0 35.0 40.0 29.0 41.0 56-57 35.99442302206352 38.5 35.0 40.0 28.5 41.0 58-59 35.785805122590425 38.0 35.0 40.0 28.0 41.0 60-61 35.56045962189769 38.0 34.0 40.0 28.0 41.0 62-63 35.26391267370569 38.0 34.0 40.0 27.5 41.0 64-65 34.94916600829504 37.0 34.0 40.0 26.5 41.0 66-67 34.63231359047892 37.0 34.0 39.0 26.0 41.0 68-69 34.27522414998965 36.0 33.0 39.0 26.0 40.5 70-71 33.92099695978941 36.0 33.0 39.0 26.0 40.0 72-73 33.53840165438645 35.0 33.0 38.0 26.0 40.0 74-75 33.15603807506059 35.0 32.0 37.0 25.0 39.5 76-77 32.17667524845568 34.0 31.0 36.0 24.5 39.0 78-79 32.58774620062546 35.0 32.0 36.0 25.0 39.0 80-81 32.440260439670794 35.0 32.0 36.0 25.0 38.0 82-83 32.14535934649247 35.0 32.0 35.5 24.5 37.0 84-85 31.82819144350905 34.0 31.0 35.0 24.0 37.0 86-87 31.55269913112719 34.0 31.0 35.0 23.0 36.0 88-89 31.303137033426317 34.0 31.0 35.0 23.0 36.0 90-91 31.066734888482657 34.0 31.0 35.0 21.0 36.0 92-93 30.871081429832103 34.0 30.5 35.0 20.0 35.0 94-95 30.668218246417908 34.0 30.0 35.0 20.0 35.0 96-97 30.477817615803055 34.0 30.0 35.0 19.5 35.0 98-99 30.261941780100617 34.0 29.0 35.0 19.0 35.0 100 27.90784011744935 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3252602082343117 1101 2 -0.36204076542052377 1101 3 -0.32745764344349126 1101 4 -0.400878279866447 1101 5 -0.2113637138176756 1101 6 -0.23115304324765162 1101 7 -0.2507650416189051 1101 8 -0.29177124095738094 1101 9 -0.708212381852519 1101 10-11 -0.42472556719624066 1101 12-13 -0.551920888145311 1101 14-15 -0.5453694466730283 1101 16-17 -0.7130758951603653 1101 18-19 -0.7106133218737014 1101 20-21 -0.7796261120027523 1101 22-23 -0.8269073693863334 1101 24-25 -0.6724065621854365 1101 26-27 -0.791381922864467 1101 28-29 -0.6906193795070337 1101 30-31 -0.8272558454196925 1101 32-33 -0.7211133210497707 1101 34-35 -0.8257939296199837 1101 36-37 -0.7664610708285906 1101 38-39 -0.6849265839220777 1101 40-41 -0.6947914741633596 1101 42-43 -0.7570935842735906 1101 44-45 -0.694253496455552 1101 46-47 -0.7662216419291639 1101 48-49 0.33225582297438905 1101 50-51 -0.40402356524499083 1101 52-53 -0.6548284356451219 1101 54-55 -0.7584573318752135 1101 56-57 -0.7443752619640946 1101 58-59 -0.942199677711713 1101 60-61 -0.8919501728279755 1101 62-63 -0.8861803561766166 1101 64-65 -0.8805804836335511 1101 66-67 -0.9280272641717815 1101 68-69 -1.1291459576998122 1101 70-71 -1.0132450622755158 1101 72-73 -0.8564471572170049 1101 74-75 -0.736196557262538 1101 76-77 -0.7674017304416623 1101 78-79 -0.7853628410166067 1101 80-81 -0.6844461710461793 1101 82-83 -0.729691498815118 1101 84-85 -0.7463239210967316 1101 86-87 -0.7960189933373911 1101 88-89 -0.6908110314490479 1101 90-91 -0.9300305631809458 1101 92-93 -0.9020128183370772 1101 94-95 -0.9388985949284034 1101 96-97 -1.0944929040320055 1101 98-99 -1.0625723898368271 1101 100 -0.851004103576134 1102 1 -0.34306304779025965 1102 2 -0.3491573992114425 1102 3 -0.3384376860225622 1102 4 -0.1635879321267879 1102 5 -0.1611354153794835 1102 6 -0.25563442454699015 1102 7 -0.25191459202342514 1102 8 -0.29158127847959037 1102 9 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0.8628254094362795 2310 92-93 0.6357711312688856 2310 94-95 0.7392842649389415 2310 96-97 0.8479754108463418 2310 98-99 0.9233923692640289 2310 100 1.1019866442408102 2311 1 0.35103352590757453 2311 2 0.4090615564462823 2311 3 0.4570448545470498 2311 4 0.27447800235846387 2311 5 0.3530165985831317 2311 6 0.36445289347256704 2311 7 0.3700221922424731 2311 8 0.21344801367923338 2311 9 0.41433559423948907 2311 10-11 0.4103316096477059 2311 12-13 0.4365162147520252 2311 14-15 0.4513698682266565 2311 16-17 0.4137228926869767 2311 18-19 0.6247244568642785 2311 20-21 0.6808272392690284 2311 22-23 0.6811298847231342 2311 24-25 0.6864770224849508 2311 26-27 0.7315925799879821 2311 28-29 0.7690228600089526 2311 30-31 0.7099678671631722 2311 32-33 0.767548639610915 2311 34-35 0.8619529955912029 2311 36-37 0.7844653015711103 2311 38-39 0.8672526947468953 2311 40-41 0.823380023298256 2311 42-43 0.7717581123777393 2311 44-45 0.8621678808541873 2311 46-47 0.872847092465193 2311 48-49 -6.083508953824332 2311 50-51 -0.9918139372137915 2311 52-53 0.4869776028997208 2311 54-55 0.8791259154986193 2311 56-57 1.028605132141962 2311 58-59 1.0768685774613544 2311 60-61 0.8239767471117716 2311 62-63 0.6854754068852813 2311 64-65 0.8438297424737584 2311 66-67 0.9420126715967214 2311 68-69 0.9013151898292548 2311 70-71 0.8884902138975406 2311 72-73 0.35053040393865587 2311 74-75 0.6833355524458753 2311 76-77 0.8068128169348086 2311 78-79 0.8302566247942238 2311 80-81 0.7656407521728639 2311 82-83 0.8652511520588959 2311 84-85 0.8522435834613162 2311 86-87 0.7995328824674566 2311 88-89 0.9075456751441457 2311 90-91 0.9980105959880525 2311 92-93 1.0996844567749626 2311 94-95 1.0903170722813158 2311 96-97 1.0435102560562264 2311 98-99 0.9748818242313284 2311 100 1.2423911773614158 2312 1 0.08051160389973688 2312 2 0.19450348276046014 2312 3 0.1693803341538498 2312 4 -0.10712534219354808 2312 5 0.06421641553362178 2312 6 0.047805584351308994 2312 7 0.1981897149294909 2312 8 0.22540231007969425 2312 9 0.14105983273524458 2312 10-11 0.17806408087304249 2312 12-13 0.21491130480284681 2312 14-15 0.18736451168945933 2312 16-17 0.43185398540317266 2312 18-19 0.2812645482750682 2312 20-21 0.253709972128604 2312 22-23 0.5245083746666594 2312 24-25 0.6614281937888791 2312 26-27 0.5797287377536264 2312 28-29 0.6807117436198737 2312 30-31 0.6839693042097679 2312 32-33 0.726474807143795 2312 34-35 0.2672429434322865 2312 36-37 0.656418512389628 2312 38-39 0.7617228842769066 2312 40-41 0.5440702854782913 2312 42-43 0.5912198376673388 2312 44-45 0.6401929134141469 2312 46-47 0.7603518643775331 2312 48-49 -4.858738462940373 2312 50-51 -0.8410172340241857 2312 52-53 0.6016669360375246 2312 54-55 0.8369091481757138 2312 56-57 0.9198273474668426 2312 58-59 0.8329098259765573 2312 60-61 0.8107968976717927 2312 62-63 0.6710532858440956 2312 64-65 0.9678379868988287 2312 66-67 0.8324071306905267 2312 68-69 0.7705692151041248 2312 70-71 0.8010823496660606 2312 72-73 0.9291029206624657 2312 74-75 0.5864795267013889 2312 76-77 0.7591083879104232 2312 78-79 0.4750959721615473 2312 80-81 0.6397652768184301 2312 82-83 0.6486696076116587 2312 84-85 0.8020484636019773 2312 86-87 0.9581370040202479 2312 88-89 0.942678120639382 2312 90-91 0.8086192056124837 2312 92-93 0.9129414964889655 2312 94-95 0.97693426100194 2312 96-97 1.1059293434656645 2312 98-99 1.084470721546971 2312 100 0.6518007137489512 2313 1 -0.0109157788662273 2313 2 0.13198682181860022 2313 3 0.22415670389084852 2313 4 0.032716443317525545 2313 5 0.13696081898668666 2313 6 0.18460360697610412 2313 7 0.1765145397110075 2313 8 0.24757873383092033 2313 9 0.4504258822587772 2313 10-11 0.24271880497658316 2313 12-13 0.053109851662803464 2313 14-15 0.14604297233642427 2313 16-17 0.08495788063304843 2313 18-19 0.4104828802493117 2313 20-21 0.36141984755501966 2313 22-23 0.5209019635799308 2313 24-25 0.31363359993130047 2313 26-27 0.4193495281618169 2313 28-29 0.30270851325443715 2313 30-31 0.5337496638326442 2313 32-33 0.4576945934816905 2313 34-35 0.33340272415899364 2313 36-37 -0.39930529136567117 2313 38-39 -0.10207473542622836 2313 40-41 0.21262963514001143 2313 42-43 0.3548018374698074 2313 44-45 0.3333545199615813 2313 46-47 0.20127882892654725 2313 48-49 -5.632473476832864 2313 50-51 -1.5253160935198977 2313 52-53 0.021440513291416607 2313 54-55 0.14720886595574711 2313 56-57 0.1579429064485396 2313 58-59 0.2915885051276774 2313 60-61 0.621806316948927 2313 62-63 0.7246538847202828 2313 64-65 0.6662786538434347 2313 66-67 0.7056262125710262 2313 68-69 0.8459996901408999 2313 70-71 0.7593425933593778 2313 72-73 0.5134658455765759 2313 74-75 0.651748247481919 2313 76-77 0.7575093745003869 2313 78-79 0.6560897378009898 2313 80-81 -0.25935397663153736 2313 82-83 0.1372365961786528 2313 84-85 0.5575165300936646 2313 86-87 0.7038366237356151 2313 88-89 0.6426909249828974 2313 90-91 0.6896006476175494 2313 92-93 0.7841592988091328 2313 94-95 0.6982682638320021 2313 96-97 0.6665793234069852 2313 98-99 1.0440448307273797 2313 100 1.237266866144239 2314 1 0.11259139365871107 2314 2 0.18076144171466524 2314 3 0.26555340643614045 2314 4 0.09959929456521621 2314 5 0.19343456181661622 2314 6 0.28000550426453685 2314 7 0.06539020499687354 2314 8 0.15262942502011612 2314 9 0.31279291573113 2314 10-11 0.30748310704974813 2314 12-13 0.31868864236857775 2314 14-15 0.2577182194151888 2314 16-17 0.5111644171026839 2314 18-19 0.5729282315769808 2314 20-21 0.5646388990984903 2314 22-23 0.2943961471141563 2314 24-25 0.5246594827880884 2314 26-27 0.5212329104864679 2314 28-29 0.5639633990558295 2314 30-31 0.41744146344984046 2314 32-33 0.34880867350496914 2314 34-35 0.21771709953784324 2314 36-37 0.23154759263815805 2314 38-39 0.28303604210883293 2314 40-41 0.01115780546683709 2314 42-43 0.1161435298265161 2314 44-45 0.4019016256348138 2314 46-47 0.48135687377845215 2314 48-49 -6.420784494269679 2314 50-51 -1.675042045011459 2314 52-53 -0.8577473697931666 2314 54-55 -0.6683515183477482 2314 56-57 -0.3207730646352758 2314 58-59 0.21345855294165972 2314 60-61 0.45035803808617914 2314 62-63 0.29118012525420767 2314 64-65 0.3798539193925734 2314 66-67 0.619804602388335 2314 68-69 0.5680007369383802 2314 70-71 0.32786965034414095 2314 72-73 0.46289516179259493 2314 74-75 0.42419840622914506 2314 76-77 0.3723595660080221 2314 78-79 0.21469397370494647 2314 80-81 0.3193930059342094 2314 82-83 0.32264750684553434 2314 84-85 0.339591125718016 2314 86-87 0.4689129175265343 2314 88-89 0.36219953589458953 2314 90-91 0.4866272991495926 2314 92-93 0.4248782703711349 2314 94-95 0.5770327783359512 2314 96-97 0.7597615749429494 2314 98-99 0.790017923681205 2314 100 -0.4565922205825501 2315 1 0.14827600904011007 2315 2 0.22639563813662988 2315 3 0.31886688827105303 2315 4 0.1441217569036084 2315 5 0.2560867771945041 2315 6 0.36364269346294975 2315 7 0.3727154831708006 2315 8 0.2971510929640857 2315 9 0.5139645220677167 2315 10-11 0.3376801294086036 2315 12-13 0.4949708263177115 2315 14-15 0.26652157293031564 2315 16-17 0.44165323307452553 2315 18-19 0.41667064040662893 2315 20-21 0.3204056799488981 2315 22-23 0.4249565422041357 2315 24-25 0.4860498235373001 2315 26-27 0.5863103284035773 2315 28-29 0.6283342240427956 2315 30-31 0.6375602128723443 2315 32-33 0.6841693850147763 2315 34-35 0.6694605653301764 2315 36-37 0.4288438085194457 2315 38-39 0.634982663821134 2315 40-41 0.6444584431983102 2315 42-43 0.609971085194772 2315 44-45 0.6905887383413969 2315 46-47 0.5601842138239945 2315 48-49 -4.862941291840002 2315 50-51 -0.8820271551333363 2315 52-53 0.2104714599467883 2315 54-55 0.10977370246254026 2315 56-57 0.6105975291411028 2315 58-59 0.7397515448359115 2315 60-61 0.5851640532407103 2315 62-63 0.16038580191310103 2315 64-65 0.4440602211144409 2315 66-67 -0.1004521208015774 2315 68-69 0.5451359750622871 2315 70-71 0.5109888525714581 2315 72-73 0.6264900306736152 2315 74-75 0.7062826379598448 2315 76-77 0.6284583201049045 2315 78-79 0.6029582177194968 2315 80-81 0.4668200669382614 2315 82-83 0.4291985849432862 2315 84-85 0.46791885438967995 2315 86-87 0.4914473785654607 2315 88-89 0.44922544444139234 2315 90-91 0.6051218882783438 2315 92-93 0.7815364871898005 2315 94-95 0.3587446899457767 2315 96-97 0.3593397515067238 2315 98-99 0.21551271642579195 2315 100 0.10594438950232998 2316 1 0.27323468141369034 2316 2 0.28297403206751426 2316 3 0.17917615725765756 2316 4 0.07006362853154258 2316 5 0.16658168862132783 2316 6 0.10967670857984757 2316 7 0.20074406805418477 2316 8 0.27524528501772494 2316 9 0.5040036907361412 2316 10-11 0.46888468823357243 2316 12-13 0.38281526396155385 2316 14-15 0.4996239885877216 2316 16-17 0.593646670960112 2316 18-19 0.22735398904131898 2316 20-21 0.5066401218268126 2316 22-23 0.6647936491724664 2316 24-25 0.6920250367431393 2316 26-27 0.632431400532333 2316 28-29 0.6841581704503383 2316 30-31 0.5911441530195987 2316 32-33 0.5446731698288474 2316 34-35 0.5080626644801427 2316 36-37 0.4456843796022625 2316 38-39 0.5933926087222972 2316 40-41 0.21769421119347498 2316 42-43 0.13045896778098864 2316 44-45 0.3074302177236987 2316 46-47 0.31378750773713193 2316 48-49 -5.313319074555203 2316 50-51 -0.9450766937870654 2316 52-53 0.37157381411930857 2316 54-55 0.654421652570349 2316 56-57 0.5173049015983864 2316 58-59 0.3896295532286018 2316 60-61 0.56935971212814 2316 62-63 0.4726880895701555 2316 64-65 0.42292409217504456 2316 66-67 0.4875586753858059 2316 68-69 0.23350184647782157 2316 70-71 -0.05417304133077039 2316 72-73 -0.07058524499527863 2316 74-75 0.13062897674436869 2316 76-77 0.20723457520011124 2316 78-79 0.25046814962498587 2316 80-81 0.15985089462267155 2316 82-83 0.07922464615149494 2316 84-85 0.35504804112626687 2316 86-87 0.4309434540130077 2316 88-89 0.3905963198883846 2316 90-91 0.3573204621084223 2316 92-93 0.3941125223367976 2316 94-95 0.5609510891252292 2316 96-97 0.599560300594721 2316 98-99 0.6412609106565519 2316 100 -0.07391358040640839 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 19.0 13 41.0 14 215.0 15 683.0 16 1674.0 17 3879.0 18 6598.0 19 9100.0 20 12078.0 21 15646.0 22 19027.0 23 19236.0 24 18497.0 25 19449.0 26 22432.0 27 27506.0 28 33801.0 29 41744.0 30 51786.0 31 64090.0 32 80883.0 33 104066.0 34 144580.0 35 202238.0 36 312643.0 37 496701.0 38 469718.0 39 72162.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3815344406861 20.845404723065503 13.4318009076322 23.341259928616196 2 15.595349025285133 21.392404107084218 40.27700200804016 22.73524485959049 3 17.316041745445563 27.902503313712483 31.594843272397167 23.186611668444794 4 11.695112921304869 17.47406858923703 39.72605905668243 31.10475943277567 5 11.50619508978481 38.75045989943533 35.981687559875795 13.76165745090407 6 28.348932082234136 39.24037358792553 18.864643917065578 13.546050412774754 7 25.90819817818263 33.44252388358143 23.99622306154188 16.653054876694068 8 25.442162300514838 36.082541110140426 21.28924493138608 17.186051657958654 9 26.65861128689358 15.26919603813966 21.996030673843332 36.07616200112343 10-11 23.924667576251007 26.965931911813424 29.718813132407174 19.390587379528398 12-13 24.060713659662515 25.381332180403586 29.47013739400404 21.08781676592986 14-15 21.9437680515441 26.609175738724726 27.120395467673852 24.32666074205732 16-17 20.51762459053398 29.221032556436548 28.56462053630939 21.696722316720077 18-19 20.326900616107395 28.417167647818786 30.666800562808138 20.589131173265677 20-21 21.132415018884693 27.9193956898467 30.605132192846035 20.343057098422573 22-23 21.18970662467296 27.623066204072344 30.300875895802527 20.886351275452167 24-25 20.829397174497068 27.785023074851743 30.653627501775162 20.731952248876027 26-27 20.530521988331042 27.87795425950805 30.64598297134349 20.945540780817424 28-29 20.489310360686318 27.75344832987369 30.923114624544567 20.834126684895423 30-31 21.109590921997412 27.723376336565426 30.776275510997575 20.390757230439586 32-33 20.9529252203714 28.031784319546087 30.4627799414476 20.552510518634914 34-35 21.12359920083534 27.96638548650849 30.559515256991972 20.350500055664206 36-37 21.188999018015494 27.67714109399607 30.211234444967644 20.922625443020788 38-39 20.758294302259507 28.281393505445614 30.363082874467757 20.597229317827125 40-41 20.717468822536247 27.654908596690298 31.030444366482424 20.59717821429103 42-43 20.615946368037854 27.822613524993283 30.264007168584605 21.297432938384254 44-45 20.302263465329002 28.339477360347352 30.33479929351281 21.023459880810837 46-47 20.571903572180876 27.945953777005112 29.95035600931819 21.531786641495817 48-49 20.786529869605225 28.188605578244854 29.78260189882287 21.242262653327057 50-51 20.705266163645838 27.986275997773408 29.78552913295016 21.522928705630594 52-53 20.5587553801905 28.493159999848892 29.492321569005657 21.45576305095495 54-55 20.759049390693175 28.266041909786505 29.558491411222338 21.416417288297986 56-57 20.817657603850876 28.367315559655758 29.18716398834997 21.627862848143398 58-59 20.43331257328139 28.176315721062224 29.731719841062286 21.6586518645941 60-61 20.904022462029115 28.7667324853119 29.182085389749403 21.14715966290959 62-63 20.781655084517546 28.41427506174727 29.530925182486616 21.27314467124857 64-65 20.544822272883128 28.048522768408425 29.400767498660578 22.00588746004787 66-67 20.563064574700206 28.20832220277863 29.008255352778207 22.22035786974295 68-69 20.33833064199615 28.18792334111621 29.27681480799943 22.196931208888213 70-71 20.550620517018995 27.92653266948102 29.432312042590375 22.090534770909613 72-73 20.629623547226434 28.368082415559186 28.789689254959022 22.212604782255358 74-75 20.582307945608715 28.54613655946997 28.74015625562428 22.131399239297032 76-77 20.48516334321407 28.267286471688806 29.02147393308061 22.22607625201651 78-79 20.31232953779148 28.336826547210826 28.875506567856817 22.47533734714088 80-81 20.326328238537155 28.652079699311265 28.455095429391715 22.566496632759865 82-83 20.882924989839648 28.300613931801106 28.632366864970333 22.184094213388907 84-85 20.48834071758627 28.60884852544206 28.250039834298207 22.65277092267346 86-87 20.16183836075381 28.794232433362176 28.733720273373624 22.310208932510385 88-89 20.504413843377456 28.304270715727153 28.626574626401275 22.56474081449412 90-91 20.705957698626435 28.23586352397077 28.60583776643912 22.45234101096367 92-93 20.70831481646076 28.374366722957962 28.392409563594345 22.524908896986933 94-95 20.261626841247498 27.890843282744736 28.63286366408669 23.214666211921077 96-97 20.507472807370693 28.023983014771424 28.492094385685203 22.97644979217268 98-99 20.165352208381023 28.242910024658173 28.40698677186777 23.18475099509303 100 20.164204984644954 27.963741732966657 28.425393232756186 23.446660049632204 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 915.0 1 649.5 2 855.0 3 1248.0 4 946.0 5 817.0 6 1165.0 7 2089.5 8 3260.5 9 3844.5 10 3323.0 11 2552.5 12 2474.5 13 2703.5 14 2981.5 15 3193.0 16 3422.5 17 3743.0 18 4097.5 19 4438.5 20 4881.0 21 5493.0 22 6235.5 23 7380.5 24 8653.0 25 10379.0 26 13659.0 27 17098.0 28 19137.5 29 22438.5 30 29524.5 31 36476.5 32 44283.5 33 54914.0 34 65703.5 35 74470.0 36 83778.0 37 92492.0 38 93674.0 39 92131.5 40 87464.5 41 81493.5 42 77444.5 43 76288.0 44 78118.5 45 81147.0 46 81024.0 47 76659.0 48 74760.5 49 74243.5 50 72654.5 51 69590.5 52 65457.0 53 60809.5 54 56995.0 55 52080.5 56 46372.5 57 40394.0 58 34885.0 59 29985.0 60 23976.0 61 18688.5 62 14801.5 63 11586.5 64 8573.5 65 6083.5 66 4377.0 67 3310.5 68 2624.5 69 1984.0 70 1474.0 71 1108.5 72 866.5 73 668.0 74 499.5 75 361.0 76 239.5 77 136.5 78 75.5 79 46.0 80 34.5 81 21.0 82 11.0 83 9.0 84 5.5 85 2.0 86 1.0 87 1.5 88 1.5 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030748695180008726 2 0.0021772919997405022 3 2.2217265303474515E-4 4 0.023461432160469087 5 4.443453060694903E-4 6 4.4434530606949026E-5 7 8.886906121389805E-5 8 0.0037325005709837183 9 0.010930894529309461 10-11 0.0010442114692633022 12-13 1.555208571243216E-4 14-15 8.886906121389805E-5 16-17 4.443453060694903E-4 18-19 6.665179591042354E-5 20-21 8.886906121389805E-5 22-23 2.6660718364169417E-4 24-25 0.0 26-27 6.665179591042354E-5 28-29 0.0012663841222980473 30-31 0.0022661610609544004 32-33 0.009331251427459296 34-35 0.01788489856929698 36-37 0.020728708528141723 38-39 0.021261922895425108 40-41 0.00526549187692346 42-43 0.005665402652386001 44-45 0.009486772284583618 46-47 0.012441668569945727 48-49 1.2263263929558827 50-51 0.019084630895684608 52-53 0.020684273997534773 54-55 0.010664287345667767 56-57 0.009242382366245397 58-59 0.008287039958195994 60-61 0.005620968121779052 62-63 0.008886906121389804 64-65 0.020684273997534773 66-67 0.03061539158818788 68-69 0.030704260649401776 70-71 0.02683845648659721 72-73 0.023927994731842053 74-75 0.009553424080494042 76-77 0.014752264161507078 78-79 0.008287039958195994 80-81 0.0030437653465760083 82-83 0.014818915957417501 84-85 0.024727816282767136 86-87 0.024039081058359425 88-89 0.03385911232249516 90-91 0.02135079195663901 92-93 0.013419228243298605 94-95 0.00708730763180837 96-97 0.003377024326128126 98-99 0.014907785018631398 100 0.033459201547032615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2250502.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.847545692524605 #Duplication Level Percentage of deduplicated Percentage of total 1 78.95470005959633 48.8315441957546 2 14.32530243104149 17.71969593326145 3 3.5625316069456043 6.610015090248942 4 1.2521842825791325 3.0977809852909624 5 0.5767262278854226 1.783455086561052 6 0.3013885936597665 1.1184086890546885 7 0.206146457582026 0.8924756718259507 8 0.13288133385810272 0.65747074939781 9 0.10326705281392605 0.5748132390697479 >10 0.46965774334852284 5.330090578201016 >50 0.05025012539819966 2.2009033958669524 >100 0.059678760878368944 7.392577619483968 >500 0.003683710954630869 1.5113943356598305 >1k 0.0013613714397548789 1.1041261608619892 >5k 2.4024201878027274E-4 1.1752482694610031 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9262 0.4115526224815619 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8892 0.39511184615699074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5575 0.2477225081337408 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2217265303474513E-5 2.2217265303474513E-5 14-15 0.0 0.0 0.0 4.4434530606949026E-5 1.3330359182084708E-4 16-17 0.0 0.0 0.0 4.4434530606949026E-5 2.6660718364169417E-4 18-19 0.0 0.0 0.0 4.4434530606949026E-5 2.6660718364169417E-4 20-21 0.0 0.0 0.0 4.4434530606949026E-5 2.6660718364169417E-4 22-23 0.0 2.2217265303474513E-5 0.0 4.4434530606949026E-5 3.1104171424864316E-4 24-25 0.0 4.4434530606949026E-5 0.0 1.9995538773127063E-4 3.554762448555922E-4 26-27 0.0 4.4434530606949026E-5 0.0 0.001177515061084149 3.554762448555922E-4 28-29 0.0 4.4434530606949026E-5 0.0 0.0076871737950021815 3.554762448555922E-4 30-31 0.0 4.4434530606949026E-5 0.0 0.028926879425123814 3.554762448555922E-4 32-33 0.0 4.4434530606949026E-5 0.0 0.07287263019539642 3.554762448555922E-4 34-35 0.0 6.665179591042354E-5 0.0 0.1420127598198091 3.554762448555922E-4 36-37 0.0 1.1108632651737256E-4 0.0 0.25012197278651604 3.554762448555922E-4 38-39 0.0 1.3330359182084708E-4 0.0 0.40637599966585236 3.554762448555922E-4 40-41 0.0 1.3330359182084708E-4 0.0 0.5734276174826771 3.554762448555922E-4 42-43 0.0 2.6660718364169417E-4 0.0 0.733125320484052 3.9991077546254125E-4 44-45 0.0 2.6660718364169417E-4 0.0 0.9316365859705968 4.443453060694903E-4 46-47 0.0 2.6660718364169417E-4 0.0 1.174271340349842 4.443453060694903E-4 48-49 0.0 2.6660718364169417E-4 0.0 1.4258374353810839 4.887798366764393E-4 50-51 0.0 2.6660718364169417E-4 0.0 1.6902228924924305 4.887798366764393E-4 52-53 0.0 2.6660718364169417E-4 0.0 1.9460102679313325 5.332143672833883E-4 54-55 0.0 2.6660718364169417E-4 0.0 2.2381450894067187 5.776488978903373E-4 56-57 0.0 2.6660718364169417E-4 0.0 2.5857342050795777 5.776488978903373E-4 58-59 0.0 2.6660718364169417E-4 0.0 2.962894500871361 5.776488978903373E-4 60-61 0.0 2.6660718364169417E-4 0.0 3.3358335162554846 6.665179591042354E-4 62-63 0.0 2.6660718364169417E-4 0.0 3.7054843763746934 6.665179591042354E-4 64-65 0.0 2.6660718364169417E-4 0.0 4.074890846575564 6.665179591042354E-4 66-67 0.0 2.6660718364169417E-4 0.0 4.494397250035769 6.665179591042354E-4 68-69 4.4434530606949026E-5 2.6660718364169417E-4 0.0 4.953761427450409 6.665179591042354E-4 70-71 4.4434530606949026E-5 2.6660718364169417E-4 0.0 5.440541710249535 6.665179591042354E-4 72-73 4.4434530606949026E-5 2.6660718364169417E-4 0.0 5.906037852887933 6.887352244077099E-4 74-75 4.4434530606949026E-5 2.6660718364169417E-4 0.0 6.358647981650316 7.109524897111844E-4 76-77 4.4434530606949026E-5 2.6660718364169417E-4 0.0 6.874221840282746 7.109524897111844E-4 78-79 4.4434530606949026E-5 2.6660718364169417E-4 0.0 7.4290758239717185 7.109524897111844E-4 80-81 4.4434530606949026E-5 2.6660718364169417E-4 0.0 7.996438128026547 7.109524897111844E-4 82-83 6.665179591042354E-5 2.6660718364169417E-4 0.0 8.548581605348495 7.998215509250825E-4 84-85 8.886906121389805E-5 2.6660718364169417E-4 0.0 9.091593786630716 7.998215509250825E-4 86-87 8.886906121389805E-5 2.6660718364169417E-4 0.0 9.6522686938292 7.998215509250825E-4 88 8.886906121389805E-5 2.6660718364169417E-4 0.0 10.098191425735235 8.442560815320315E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4395 0.0 57.339127 1 TACCTGG 1940 0.0 52.71756 2 ACCTGGG 2245 0.0 44.305603 3 GTACCTG 2700 0.0 38.585567 1 GTATCAA 7660 0.0 35.1044 1 TAACGCA 215 0.0 32.733463 4 CCTGGGG 1855 0.0 32.37466 4 GTACATG 8950 0.0 30.621433 1 TACATGG 9545 0.0 28.26132 2 ACATGGG 9220 0.0 28.140602 3 ATCAACG 9475 0.0 27.581324 3 CAACGCA 9510 0.0 27.176556 5 TCAACGC 9865 0.0 26.348253 4 TGGTATC 2190 0.0 26.134624 2 AACGCAG 9990 0.0 26.105536 6 TATCAAC 10310 0.0 25.390781 2 CATGGGG 4980 0.0 25.249023 4 ATGGGGG 3415 0.0 23.761765 5 ACGCAGA 11090 0.0 23.473873 7 GTGGTAT 2480 0.0 23.464195 1 >>END_MODULE