FastQCFastQC Report
Mon 6 Jun 2016
ERR1211321_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211321_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7995415
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254000.31768207153724975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197290.24675392084088194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116370.1455459160031093No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129300.052.2359731
GTATCAA227350.040.2795641
CAACGCA279900.032.0639765
ATCAACG281300.031.9051973
TATCAAC288000.031.4200822
TCAACGC291650.030.8209024
AACGCAG298300.030.4325316
TACCTGG64350.028.2119752
ACGCAGA337400.026.5583257
GTACATG308900.026.2350041
CGCAGAG347200.025.7526268
GTGGTAT60500.025.5772761
TACATGG316350.025.2592412
ACATGGG323900.024.082133
GTACTAG24750.023.9446871
GTACCTG78050.023.8033451
TGGTATC62950.023.0117552
GCAGAGT380100.022.9075189
CATGGGG222500.022.3644544
ACCTGGG82150.021.4497133
Produced by FastQC (version 0.11.5)