##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211321_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7995415 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.235215933131677 31.0 31.0 34.0 28.0 34.0 2 31.249038480178953 31.0 31.0 34.0 28.0 34.0 3 31.298120110088096 31.0 31.0 34.0 27.0 34.0 4 34.90214466666208 37.0 35.0 37.0 32.0 37.0 5 34.94172785277562 37.0 35.0 37.0 32.0 37.0 6 35.006370401036094 37.0 35.0 37.0 32.0 37.0 7 34.93242452080349 37.0 35.0 37.0 32.0 37.0 8 34.929255329460695 37.0 35.0 37.0 32.0 37.0 9 36.46034958785754 39.0 37.0 39.0 32.0 39.0 10-11 36.387840718711914 39.0 37.0 39.0 32.0 39.0 12-13 36.29836806469708 39.0 37.0 39.0 31.0 39.0 14-15 37.40974195835989 40.0 37.0 41.0 31.0 41.0 16-17 37.4047502850071 40.0 37.0 41.0 31.0 41.0 18-19 37.39413620181067 40.0 37.0 41.0 31.0 41.0 20-21 37.35505874304211 40.0 37.0 41.0 31.0 41.0 22-23 37.23123152456752 40.0 36.5 41.0 31.0 41.0 24-25 37.11781808699111 40.0 36.0 41.0 30.0 41.0 26-27 36.970640923579325 40.0 36.0 41.0 30.0 41.0 28-29 36.82454494232007 39.0 36.0 41.0 30.0 41.0 30-31 36.63653756559228 39.0 36.0 41.0 30.0 41.0 32-33 36.51354232394441 39.0 35.0 41.0 29.5 41.0 34-35 36.36798627713509 39.0 35.0 41.0 29.0 41.0 36-37 36.272338458979306 39.0 35.0 40.5 28.5 41.0 38-39 36.11787468192708 39.0 35.0 40.0 28.0 41.0 40-41 35.94699424607728 38.5 35.0 40.0 27.0 41.0 42-43 35.79287710519091 38.0 35.0 40.0 27.0 41.0 44-45 35.65225801287363 38.0 34.5 40.0 26.5 41.0 46-47 35.48657618147401 38.0 34.0 40.0 26.0 41.0 48-49 35.24556986723016 38.0 34.0 40.0 25.0 41.0 50-51 34.57623269086095 37.5 33.5 39.5 24.0 40.5 52-53 34.82320986465368 38.0 33.5 40.0 24.5 40.5 54-55 35.17659270969674 38.0 34.0 40.0 26.0 41.0 56-57 35.07460557081777 38.0 34.0 40.0 25.5 41.0 58-59 34.84674998108291 38.0 33.5 40.0 25.0 41.0 60-61 34.57533792805002 37.0 33.0 40.0 25.0 41.0 62-63 34.29072724805404 37.0 33.0 40.0 24.0 41.0 64-65 34.00565392790743 36.0 33.0 39.5 23.0 41.0 66-67 33.77591425835932 36.0 32.0 39.0 23.0 41.0 68-69 33.426121470868 35.0 32.0 39.0 23.0 41.0 70-71 33.07509522645166 35.0 32.0 38.5 22.5 40.0 72-73 32.706839544914175 35.0 31.0 37.5 22.0 40.0 74-75 32.336673518510295 35.0 31.0 37.0 21.0 39.0 76-77 31.981293841532928 35.0 31.0 36.5 20.0 39.0 78-79 31.620797169377703 35.0 30.5 36.0 20.0 38.5 80-81 31.28797229412107 34.0 30.0 35.5 20.0 37.0 82-83 30.980440602520318 34.0 30.0 35.0 19.0 37.0 84-85 30.681612211498717 34.0 29.5 35.0 18.0 36.5 86-87 30.425717364264393 34.0 29.0 35.0 18.0 36.0 88-89 30.165133079396128 34.0 29.0 35.0 18.0 36.0 90-91 29.908175935833224 34.0 29.0 35.0 17.0 35.5 92-93 29.669727274944453 34.0 29.0 35.0 15.5 35.0 94-95 29.44564491023918 34.0 27.5 35.0 15.0 35.0 96-97 29.235624980066703 34.0 27.0 35.0 12.5 35.0 98-99 28.991925935051526 33.0 27.0 35.0 10.5 35.0 100 27.293501463025997 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.30291651146959353 1101 2 -0.2860170946890044 1101 3 -0.3723974406514401 1101 4 -0.24793649558842645 1101 5 -0.2902472553130693 1101 6 -0.30706539291339396 1101 7 -0.28033036961410573 1101 8 -0.4208470770660213 1101 9 -0.5121413506237431 1101 10-11 -0.46905993206929963 1101 12-13 -0.45673767455138403 1101 14-15 -0.508115008367497 1101 16-17 -0.5248849379228702 1101 18-19 -0.6283347003046558 1101 20-21 -0.6265901843595927 1101 22-23 -0.6602756371574969 1101 24-25 -0.6413683401118675 1101 26-27 -0.7024865659321833 1101 28-29 -0.7454092689599605 1101 30-31 -0.7308507358315026 1101 32-33 -0.786744386667884 1101 34-35 -0.7665152613543569 1101 36-37 -0.8949040177940049 1101 38-39 -0.8671150399735197 1101 40-41 -0.841581163724129 1101 42-43 -0.9038795690928296 1101 44-45 -1.002069376989759 1101 46-47 -0.8516101659417998 1101 48-49 -0.8928523562203381 1101 50-51 -0.9069638338429726 1101 52-53 -0.674867047046213 1101 54-55 -0.6167288156932926 1101 56-57 -0.5959031557952059 1101 58-59 -0.7531890750041086 1101 60-61 -0.7140408107424392 1101 62-63 -0.733100479919969 1101 64-65 -0.7811053922307565 1101 66-67 -0.7922792460762835 1101 68-69 -0.7510778145226951 1101 70-71 -0.778631799234951 1101 72-73 -0.9521338399058159 1101 74-75 -0.8743397720690282 1101 76-77 -0.9064215062223369 1101 78-79 -0.8926129464577137 1101 80-81 -0.9758845968071164 1101 82-83 -1.0687113417550478 1101 84-85 -1.011620567355756 1101 86-87 -1.0023731782480567 1101 88-89 -1.0308811685394588 1101 90-91 -1.0241114528436732 1101 92-93 -1.0389598307615948 1101 94-95 -1.1732170362349166 1101 96-97 -1.2424144733011495 1101 98-99 -1.256804051342396 1101 100 -1.2094278258749505 1102 1 -0.4151649014518064 1102 2 -0.4867035927585839 1102 3 -0.4609270970855377 1102 4 -0.28355288740008433 1102 5 -0.27243377189847706 1102 6 -0.3365735858795915 1102 7 -0.3066623923324485 1102 8 -0.3243398488315634 1102 9 -0.3806984257495074 1102 10-11 -0.4102587687410164 1102 12-13 -0.4443051570978369 1102 14-15 -0.555878926636737 1102 16-17 -0.5433982431655977 1102 18-19 -0.5545911517334474 1102 20-21 -0.6380870609117366 1102 22-23 -0.6420783989550358 1102 24-25 -0.6158005628392971 1102 26-27 -0.604536993113264 1102 28-29 -0.6760125488951871 1102 30-31 -0.7255268253350877 1102 32-33 -0.7547973159668331 1102 34-35 -0.7175004941249981 1102 36-37 -0.9171994952369573 1102 38-39 -0.9835994498398222 1102 40-41 -1.0115250550480752 1102 42-43 -0.9224485745181639 1102 44-45 -0.9093095608347213 1102 46-47 -1.0044986537181089 1102 48-49 -0.9053822630952837 1102 50-51 -0.9193932398744025 1102 52-53 -0.7845341783264672 1102 54-55 -0.6892866642842392 1102 56-57 -0.7859529805438754 1102 58-59 -0.7582550428797248 1102 60-61 -0.8513041183712247 1102 62-63 -0.8603779550180874 1102 64-65 -0.8417152653267266 1102 66-67 -0.7737672770271047 1102 68-69 -0.7890516335280253 1102 70-71 -0.8445543015360713 1102 72-73 -0.8154706931547295 1102 74-75 -0.8868650977089381 1102 76-77 -0.8805296829251539 1102 78-79 -0.8488877495505065 1102 80-81 -0.9506564608467478 1102 82-83 -0.9821522272161545 1102 84-85 -1.0415247699135364 1102 86-87 -0.9951803529202436 1102 88-89 -0.9610354110876038 1102 90-91 -1.0007453025967301 1102 92-93 -1.0556982701104332 1102 94-95 -0.9481228823107628 1102 96-97 -1.092023884837694 1102 98-99 -1.0408231001176595 1102 100 -1.1333190429220323 1103 1 -0.4399352057762229 1103 2 -0.5461889004517104 1103 3 -0.5080976233484158 1103 4 -0.35787646323856137 1103 5 -0.41657777670843643 1103 6 -0.5452291859109124 1103 7 -0.5259986842008573 1103 8 -0.4414775386416281 1103 9 -0.7040415961636413 1103 10-11 -0.658217050883195 1103 12-13 -0.7804946592925646 1103 14-15 -0.7914421003180792 1103 16-17 -0.7356679505954133 1103 18-19 -0.7688784885782312 1103 20-21 -0.908804281731797 1103 22-23 -0.9084128184593325 1103 24-25 -0.8744880133598727 1103 26-27 -0.9141432746677225 1103 28-29 -0.9067723401415719 1103 30-31 -0.9510207979079084 1103 32-33 -1.0908108913316639 1103 34-35 -1.1741645927754902 1103 36-37 -1.0691342553819894 1103 38-39 -1.11678103822711 1103 40-41 -1.095745385036544 1103 42-43 -1.150340178458535 1103 44-45 -1.2217331849080821 1103 46-47 -1.2066381946264144 1103 48-49 -1.2434773331808628 1103 50-51 -1.1603953558295146 1103 52-53 -1.1002349229222759 1103 54-55 -1.156434552050655 1103 56-57 -1.1281012791284368 1103 58-59 -1.1517693359680194 1103 60-61 -1.0962784108215473 1103 62-63 -1.165073074788502 1103 64-65 -1.1628469291595565 1103 66-67 -1.151792517694048 1103 68-69 -1.073487908867044 1103 70-71 -1.0876294058597153 1103 72-73 -1.217066504162922 1103 74-75 -1.090133035237514 1103 76-77 -1.0627983367272513 1103 78-79 -1.07545241087686 1103 80-81 -1.1102248504686116 1103 82-83 -1.152917054609702 1103 84-85 -1.1691602649880721 1103 86-87 -1.3027136944377204 1103 88-89 -1.4266940589062536 1103 90-91 -1.3412008854220154 1103 92-93 -1.3301708521073188 1103 94-95 -1.4887960054276448 1103 96-97 -1.5955842765552113 1103 98-99 -1.6798594754063174 1103 100 -1.5708392092598835 1104 1 -0.5266258084279265 1104 2 -0.5115935486499623 1104 3 -0.5201322585773873 1104 4 -0.36242184075518225 1104 5 -0.30602145340278497 1104 6 -0.3399835079791842 1104 7 -0.3655479269365145 1104 8 -0.42459779832547184 1104 9 -0.5618517740605498 1104 10-11 -0.5085579871212644 1104 12-13 -0.525751634141578 1104 14-15 -0.683976306029848 1104 16-17 -0.7007715348827972 1104 18-19 -0.7460370146568565 1104 20-21 -0.7567311192311763 1104 22-23 -0.6771645245930671 1104 24-25 -0.822877863719782 1104 26-27 -0.8232228741646068 1104 28-29 -0.8412182251866653 1104 30-31 -0.8549196384971296 1104 32-33 -0.9353820912035289 1104 34-35 -0.834204851479349 1104 36-37 -0.9532765248809696 1104 38-39 -0.9856954292660376 1104 40-41 -1.0448592413263071 1104 42-43 -1.0897600770109435 1104 44-45 -1.0831213848877326 1104 46-47 -1.112019220589083 1104 48-49 -1.052645731521821 1104 50-51 -1.0008278420296293 1104 52-53 -0.9307176100043435 1104 54-55 -0.8926687993342313 1104 56-57 -0.907842863840159 1104 58-59 -0.9462382622311836 1104 60-61 -0.886366058282583 1104 62-63 -0.796480383465969 1104 64-65 -0.9098879957682726 1104 66-67 -0.9008023040626227 1104 68-69 -0.8819354194054938 1104 70-71 -0.9168788929422931 1104 72-73 -0.8912312309573451 1104 74-75 -0.9103007730583954 1104 76-77 -0.8771686914316668 1104 78-79 -0.9706701428343045 1104 80-81 -0.9960615897750564 1104 82-83 -1.0731628133996978 1104 84-85 -1.0678563164220662 1104 86-87 -1.0434641424748534 1104 88-89 -1.0478944585301058 1104 90-91 -0.9412454107311881 1104 92-93 -0.9781965400538475 1104 94-95 -1.127897303023758 1104 96-97 -1.1453835495279883 1104 98-99 -1.1264229165639144 1104 100 -1.251422078287593 1105 1 -0.3802024191739335 1105 2 -0.3704511731726008 1105 3 -0.34542525630247667 1105 4 -0.3034227808420127 1105 5 -0.21578562929355627 1105 6 -0.2613037224645538 1105 7 -0.29202523627918 1105 8 -0.30663030235541555 1105 9 -0.4950761838625155 1105 10-11 -0.48552882847134526 1105 12-13 -0.5149708099050727 1105 14-15 -0.5356372587719136 1105 16-17 -0.5217700284325559 1105 18-19 -0.5931408472671293 1105 20-21 -0.6558776108170434 1105 22-23 -0.7005999410060539 1105 24-25 -0.5715452998600057 1105 26-27 -0.5942401625208404 1105 28-29 -0.6511896112667941 1105 30-31 -0.743889064069819 1105 32-33 -0.7154070637876799 1105 34-35 -0.7531405820069423 1105 36-37 -0.7041626453607606 1105 38-39 -0.69037985286203 1105 40-41 -0.7215621885531291 1105 42-43 -0.7886396128559241 1105 44-45 -0.7372111990204004 1105 46-47 -0.7476623849725641 1105 48-49 -0.6546883539735546 1105 50-51 -0.6098767251775854 1105 52-53 -0.5269749177508203 1105 54-55 -0.489365695212129 1105 56-57 -0.5503555398222133 1105 58-59 -0.46106139803577406 1105 60-61 -0.5219927213710349 1105 62-63 -0.5654187521404523 1105 64-65 -0.43958158919572554 1105 66-67 -0.47847333787849067 1105 68-69 -0.5270979299091465 1105 70-71 -0.4205804649425673 1105 72-73 -0.4837656985758443 1105 74-75 -0.570088563032833 1105 76-77 -0.5628206693047986 1105 78-79 -0.4944277371994694 1105 80-81 -0.5116037868361438 1105 82-83 -0.518450962253894 1105 84-85 -0.44849815086859834 1105 86-87 -0.4963785305908175 1105 88-89 -0.5495409543770897 1105 90-91 -0.6190971833626726 1105 92-93 -0.6476349373012269 1105 94-95 -0.6787175175934124 1105 96-97 -0.7618819526203175 1105 98-99 -0.7006895328014267 1105 100 -0.7035513250461776 1106 1 -0.5446371577635425 1106 2 -0.48086577814617826 1106 3 -0.5046229664505084 1106 4 -0.4255583077118956 1106 5 -0.3625204569623932 1106 6 -0.36183649761938597 1106 7 -0.38867431473700265 1106 8 -0.4842571087508176 1106 9 -0.570470842997544 1106 10-11 -0.5668321563598582 1106 12-13 -0.5910065332790779 1106 14-15 -0.665762440314424 1106 16-17 -0.7032996756222545 1106 18-19 -0.6958626625859523 1106 20-21 -0.7253870193705794 1106 22-23 -0.6704788080925681 1106 24-25 -0.7210591778745297 1106 26-27 -0.7889516479177345 1106 28-29 -0.8107094363509901 1106 30-31 -0.837191570208752 1106 32-33 -0.8480632772261387 1106 34-35 -0.8379015705825736 1106 36-37 -0.8777479524633591 1106 38-39 -0.9775696618898024 1106 40-41 -0.9004168379808348 1106 42-43 -0.8747242119181209 1106 44-45 -0.7929609802206556 1106 46-47 -0.9165148823332103 1106 48-49 -0.8717390188551946 1106 50-51 -0.8768541061166601 1106 52-53 -0.715833532204762 1106 54-55 -0.8683525873294968 1106 56-57 -0.7205170218797008 1106 58-59 -0.7683002058075274 1106 60-61 -0.8182655497442042 1106 62-63 -0.7023729187480185 1106 64-65 -0.7687476683728249 1106 66-67 -0.8158411879503404 1106 68-69 -0.759882713151093 1106 70-71 -0.760620269259455 1106 72-73 -0.6810099818600079 1106 74-75 -0.7172360972345899 1106 76-77 -0.6595135218025341 1106 78-79 -0.6133885095538893 1106 80-81 -0.8018783599711519 1106 82-83 -0.7713153109191921 1106 84-85 -0.7595682673930284 1106 86-87 -0.7806818268229918 1106 88-89 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1107 46-47 -0.8694250725156394 1107 48-49 -0.8301654327671102 1107 50-51 -0.7860491758917405 1107 52-53 -0.7589675276075454 1107 54-55 -0.7739051296442909 1107 56-57 -0.7204060484246 1107 58-59 -0.7181848422443906 1107 60-61 -0.7861827599535403 1107 62-63 -0.7785353322921296 1107 64-65 -0.7967980172459121 1107 66-67 -0.7426552492314613 1107 68-69 -0.7428238128266287 1107 70-71 -0.8055640302710074 1107 72-73 -0.7345126109036215 1107 74-75 -0.7260271296580925 1107 76-77 -0.7058786643631159 1107 78-79 -0.8566766055801587 1107 80-81 -0.6821060135611319 1107 82-83 -0.7021060453865715 1107 84-85 -0.7637891282466853 1107 86-87 -0.7865173906045335 1107 88-89 -0.8423267180513498 1107 90-91 -0.8243345186700743 1107 92-93 -0.7272942902284285 1107 94-95 -0.7612872160162958 1107 96-97 -0.8503445085711263 1107 98-99 -0.883954708246204 1107 100 -0.9095434326274301 1108 1 -0.48023179730542864 1108 2 -0.5016674637492109 1108 3 -0.5228581428841785 1108 4 -0.34575083234827986 1108 5 -0.4340029454407812 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62-63 1.000622804989753 2205 64-65 0.9889182159703793 2205 66-67 0.9496111730611005 2205 68-69 1.0012771897695387 2205 70-71 0.9672716449842156 2205 72-73 0.9416964083438231 2205 74-75 0.8990087022503559 2205 76-77 0.8720482870545361 2205 78-79 0.8816937425367435 2205 80-81 0.9488606175010794 2205 82-83 1.0050971500508794 2205 84-85 0.9628098419874718 2205 86-87 0.9345983962519568 2205 88-89 0.9577430699912135 2205 90-91 0.9397070527204683 2205 92-93 1.015824955323513 2205 94-95 1.0401929040237263 2205 96-97 1.0024386801641931 2205 98-99 0.954388742846028 2205 100 1.2063590544020144 2206 1 0.7251763667504001 2206 2 0.7114578140862626 2206 3 0.6832318704593021 2206 4 0.6448528780764207 2206 5 0.6341578713558462 2206 6 0.6355138080829477 2206 7 0.6120716655171492 2206 8 0.6761688971889868 2206 9 0.8143978019081004 2206 10-11 0.8414762515799907 2206 12-13 0.8685409137197979 2206 14-15 1.106480375240281 2206 16-17 1.0010746770973498 2206 18-19 1.0789079578830822 2206 20-21 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28-29 0.9314147102557229 2311 30-31 0.9195813574503333 2311 32-33 0.9602095345470261 2311 34-35 0.9944026796657397 2311 36-37 0.9475339963177802 2311 38-39 1.025741487423538 2311 40-41 1.032787484760398 2311 42-43 1.0238889116259386 2311 44-45 1.072572318623898 2311 46-47 1.0669860222417569 2311 48-49 1.1070767856877097 2311 50-51 1.0128144299344441 2311 52-53 0.9572146814749729 2311 54-55 0.9231853457431356 2311 56-57 0.9031095335526018 2311 58-59 0.9580574800881365 2311 60-61 0.9473468446402151 2311 62-63 0.9695143884651074 2311 64-65 0.913473006521393 2311 66-67 0.9348610323755366 2311 68-69 0.9015763041786116 2311 70-71 0.8355712691646602 2311 72-73 0.8532467270584334 2311 74-75 0.9015798176039098 2311 76-77 0.9528827210157864 2311 78-79 0.8864442868564559 2311 80-81 0.8943489943034386 2311 82-83 0.9216776250256586 2311 84-85 0.9576833461710947 2311 86-87 0.917731888328511 2311 88-89 0.9804962350986948 2311 90-91 1.0169806124941907 2311 92-93 0.9809079378143934 2311 94-95 1.1220811114365645 2311 96-97 1.128726180269254 2311 98-99 1.174718725005981 2311 100 1.2216426176569577 2312 1 0.4704808938119953 2312 2 0.45799041657692285 2312 3 0.4955933330818958 2312 4 0.38762492116919844 2312 5 0.35489584635340776 2312 6 0.4219455283108715 2312 7 0.47227129286682157 2312 8 0.46768470015398833 2312 9 0.5664598644844858 2312 10-11 0.595944238231155 2312 12-13 0.594498868095819 2312 14-15 0.7711216591092409 2312 16-17 0.7454467398597089 2312 18-19 0.7055962211629705 2312 20-21 0.7426114002156012 2312 22-23 0.7997055763392495 2312 24-25 0.8039585145013675 2312 26-27 0.8488564076249858 2312 28-29 0.8131270366501298 2312 30-31 0.9092940583836935 2312 32-33 0.8573422424875403 2312 34-35 0.8468504581675447 2312 36-37 0.8489370103590019 2312 38-39 0.8948058318837866 2312 40-41 0.9139521549644414 2312 42-43 0.8950555798089894 2312 44-45 0.8847098610751729 2312 46-47 0.8872482819825294 2312 48-49 0.9306538101016884 2312 50-51 0.9141530685714301 2312 52-53 0.7491416615578004 2312 54-55 0.7376301002490919 2312 56-57 0.6847288954166473 2312 58-59 0.6763854258762265 2312 60-61 0.7833921776234902 2312 62-63 0.7338827293358463 2312 64-65 0.7398132653386114 2312 66-67 0.7450899550836496 2312 68-69 0.7827782555567566 2312 70-71 0.7639131791547484 2312 72-73 0.7835627877298563 2312 74-75 0.7698854141491651 2312 76-77 0.8388867552158921 2312 78-79 0.8602551986389066 2312 80-81 0.902341634063081 2312 82-83 0.8942023849555767 2312 84-85 0.8513260375113383 2312 86-87 0.9906154220865702 2312 88-89 0.9970416080026361 2312 90-91 1.0289971620270144 2312 92-93 1.0368464785399567 2312 94-95 1.103208882400878 2312 96-97 1.0446673682622674 2312 98-99 1.1696753058788367 2312 100 1.324415551247231 2313 1 0.4912591344168078 2313 2 0.5097247450428277 2313 3 0.5523999986687507 2313 4 0.4693804660198637 2313 5 0.4855433034339214 2313 6 0.45541930572407097 2313 7 0.48874012746471607 2313 8 0.48223643105304603 2313 9 0.618886028701489 2313 10-11 0.6291562116112033 2313 12-13 0.6781871267665522 2313 14-15 0.8393766911704432 2313 16-17 0.8243691863304647 2313 18-19 0.7421028643182623 2313 20-21 0.746898023883432 2313 22-23 0.766943433614685 2313 24-25 0.7586592927665592 2313 26-27 0.7862802653411052 2313 28-29 0.8259597315777256 2313 30-31 0.8871090927192711 2313 32-33 0.8887347869272944 2313 34-35 0.8871596002850666 2313 36-37 0.9447362821365957 2313 38-39 0.9737627411627088 2313 40-41 1.0018582623634913 2313 42-43 0.8287921762145558 2313 44-45 0.9745647625272724 2313 46-47 0.9955114640940579 2313 48-49 1.1091176420595446 2313 50-51 1.0259285460401415 2313 52-53 0.8684041147911685 2313 54-55 0.8141173015107057 2313 56-57 0.8498655050118629 2313 58-59 0.86690741953052 2313 60-61 0.859391951343035 2313 62-63 0.9305241798104689 2313 64-65 0.9480529816408705 2313 66-67 0.9377195019428086 2313 68-69 0.8774360417399762 2313 70-71 0.9005570443162298 2313 72-73 0.9196690198242479 2313 74-75 0.9304066111448321 2313 76-77 0.9721473948063171 2313 78-79 0.9637455831718817 2313 80-81 0.937470048163842 2313 82-83 0.9264047149813379 2313 84-85 0.9799716321537133 2313 86-87 1.0110403134337993 2313 88-89 0.9700671979817415 2313 90-91 1.1097803289208983 2313 92-93 1.0832496070637987 2313 94-95 1.1523966576580946 2313 96-97 1.262976914603577 2313 98-99 1.2791982247733387 2313 100 1.2346015917076762 2314 1 0.43662544885923893 2314 2 0.2947587518537027 2314 3 0.3844022039847985 2314 4 0.29140865877891997 2314 5 0.32366003326031745 2314 6 0.3316576645892013 2314 7 0.41810398208175314 2314 8 0.3864179295995669 2314 9 0.49830892183296527 2314 10-11 0.5418886564345797 2314 12-13 0.5567901474627419 2314 14-15 0.6588402595557952 2314 16-17 0.6632935290010735 2314 18-19 0.6185152388593238 2314 20-21 0.6563060791526496 2314 22-23 0.685697545649731 2314 24-25 0.7478023873493029 2314 26-27 0.7556800832749033 2314 28-29 0.7628236525792076 2314 30-31 0.7607947920166893 2314 32-33 0.8417838131186741 2314 34-35 0.8466895052540977 2314 36-37 0.8509470753603523 2314 38-39 0.8969264622155251 2314 40-41 0.9448370835817883 2314 42-43 0.864186823664383 2314 44-45 0.9543566387153177 2314 46-47 0.8194094728046863 2314 48-49 0.7872559970878399 2314 50-51 0.8815546712159872 2314 52-53 0.8418212463888111 2314 54-55 0.866159907179096 2314 56-57 0.8533623322747843 2314 58-59 0.7921751892875548 2314 60-61 0.6301598922430429 2314 62-63 0.7783641063356939 2314 64-65 0.8144226529223317 2314 66-67 0.823858743545884 2314 68-69 0.8626076530679114 2314 70-71 0.8796023455109108 2314 72-73 0.8964364358218546 2314 74-75 0.9001023412731897 2314 76-77 0.8800993216261865 2314 78-79 0.848857452547275 2314 80-81 0.9079133359024745 2314 82-83 0.9595048825683818 2314 84-85 0.9694785493201827 2314 86-87 0.9993597842966722 2314 88-89 0.9170554372479423 2314 90-91 0.9182125889463997 2314 92-93 1.0426366453131806 2314 94-95 1.0844232075972684 2314 96-97 1.0654546952941395 2314 98-99 1.1292656398079828 2314 100 1.14992715679535 2315 1 0.22934894549891283 2315 2 0.25124502971063833 2315 3 0.294883803649828 2315 4 0.2166615214711669 2315 5 0.22819464946781665 2315 6 0.2537324580846274 2315 7 0.2146158681766437 2315 8 0.23217175176590388 2315 9 0.04499200071528264 2315 10-11 0.254248091577324 2315 12-13 0.30038268400965507 2315 14-15 0.40375195182286916 2315 16-17 0.2111767082912479 2315 18-19 0.3783798671030141 2315 20-21 0.3117578089833657 2315 22-23 0.25655075790706405 2315 24-25 0.48734015735438163 2315 26-27 0.5113262012004824 2315 28-29 0.5084822910066435 2315 30-31 0.4816292296283464 2315 32-33 0.5640490995960974 2315 34-35 0.49146075382591903 2315 36-37 0.5830807355977896 2315 38-39 0.6375469861904506 2315 40-41 0.7460468612608295 2315 42-43 0.6973551339002242 2315 44-45 0.7755907478510764 2315 46-47 0.7281771051519357 2315 48-49 0.6350486071600443 2315 50-51 0.5962688847349327 2315 52-53 0.2830081910417803 2315 54-55 0.48094636742166585 2315 56-57 0.46408257371619754 2315 58-59 0.5131412046183712 2315 60-61 0.5524305955718987 2315 62-63 0.5435014242477152 2315 64-65 0.5964673883831608 2315 66-67 0.5056926521012599 2315 68-69 0.5268341947591253 2315 70-71 0.5141609409789325 2315 72-73 0.5456661822967348 2315 74-75 0.2733323522930178 2315 76-77 0.49264957135547505 2315 78-79 0.6051925549782702 2315 80-81 0.6323838826581039 2315 82-83 0.5348107153217185 2315 84-85 0.575974873677211 2315 86-87 0.5549611924673741 2315 88-89 0.4751332956458647 2315 90-91 0.6737150605781572 2315 92-93 0.7624754311190998 2315 94-95 0.7607524229842682 2315 96-97 0.753743352695242 2315 98-99 0.8674257265484258 2315 100 0.8287170671486983 2316 1 0.1049721650468527 2316 2 0.10415049785059693 2316 3 0.0936056639658247 2316 4 -0.011339410714505505 2316 5 -0.07599187157494214 2316 6 -0.11753639466807897 2316 7 -0.16350923254560712 2316 8 -0.07490766484038147 2316 9 0.013592624451227664 2316 10-11 -0.009174498899412242 2316 12-13 -0.03163131323332635 2316 14-15 -0.18441296458969703 2316 16-17 0.01927574593339898 2316 18-19 0.1561988919196864 2316 20-21 0.15072858380521126 2316 22-23 0.06134947621940512 2316 24-25 0.02168958481625083 2316 26-27 -0.0572503872330401 2316 28-29 -0.04895585065889918 2316 30-31 -0.6387638086579628 2316 32-33 -0.41736097768841063 2316 34-35 -0.4397849541656953 2316 36-37 -0.23005720596093937 2316 38-39 -0.11945253615253648 2316 40-41 0.05111168866694271 2316 42-43 0.06600802150209262 2316 44-45 -0.06289460877008679 2316 46-47 -0.1552573659355332 2316 48-49 -0.10374999449049227 2316 50-51 0.0502263125797171 2316 52-53 -0.38100832796765616 2316 54-55 -0.25761603344227524 2316 56-57 -0.2282115539897518 2316 58-59 -0.6776690885359287 2316 60-61 -0.1670587705585902 2316 62-63 -0.16747730717062126 2316 64-65 -0.36543024874748653 2316 66-67 -0.6956103622026291 2316 68-69 -0.6716986395118241 2316 70-71 -0.42800831746567525 2316 72-73 -0.7117931962071964 2316 74-75 -0.5017136319371502 2316 76-77 -0.15934407426848196 2316 78-79 -0.08253705503193132 2316 80-81 -0.17700287530501413 2316 82-83 -0.035136832215385994 2316 84-85 0.0729929128934721 2316 86-87 0.2112303844396024 2316 88-89 0.2162204399378318 2316 90-91 -0.4110452815232044 2316 92-93 -0.5158198647792815 2316 94-95 -0.04123579035976377 2316 96-97 0.3175756354237542 2316 98-99 0.2954626252058752 2316 100 0.15969911414819293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 221.0 1 589.0 2 362.0 3 319.0 4 316.0 5 322.0 6 343.0 7 903.0 8 2394.0 9 4156.0 10 6484.0 11 11246.0 12 17394.0 13 22355.0 14 26052.0 15 29746.0 16 34356.0 17 40715.0 18 47798.0 19 55609.0 20 66622.0 21 79000.0 22 89270.0 23 92772.0 24 94519.0 25 99608.0 26 108779.0 27 123197.0 28 144686.0 29 170244.0 30 203865.0 31 248834.0 32 311276.0 33 398809.0 34 540390.0 35 741998.0 36 1078292.0 37 1506365.0 38 1310737.0 39 284291.0 40 181.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.52347740581953 18.59726050018578 13.262078982364267 23.61718311163043 2 15.933760000200333 19.65913394931458 40.305607499453785 24.101498551031305 3 19.305250364207286 25.11552932834997 29.094601826302835 26.484618481139904 4 13.096668033522066 16.121817589102253 37.33565523620287 33.44585914117281 5 13.893160920877465 37.11462581127952 34.162107539116924 14.830105728726096 6 31.758057159803073 36.05494628560131 17.765473506923655 14.421523047671958 7 28.33424427433539 31.90154179267183 21.59244249309537 18.171771439897412 8 26.45095138148161 34.98198584266781 19.759193976721416 18.807868799129164 9 26.74722162585245 16.081627924317925 20.210291657345785 36.96085879248384 10-11 25.518303153904142 25.855845654819063 27.57122793627756 21.054623254999232 12-13 25.890049550714206 23.458619457684083 27.84773034932606 22.803600642275647 14-15 23.353614057287263 24.931866038250103 25.51766766315603 26.196852241306605 16-17 22.747298642668117 27.84557298453738 26.30225123433334 23.104877138461166 18-19 22.546162738220698 27.038769555846027 28.31474553862273 22.100322167310544 20-21 23.41768164327952 26.252093681562943 28.195758436243935 22.1344662389136 22-23 23.411507960267826 25.97415467963819 27.99491770138605 22.619419658707933 24-25 22.977333276763208 26.11569867296748 28.266001742409934 22.64096630785938 26-27 22.893327942094995 26.30693416119127 28.022938542761295 22.77679935395244 28-29 22.71346151211098 26.43562310291315 28.161692536987893 22.689222847987974 30-31 23.148512714310073 26.30182428490315 28.06922365829891 22.480439342487866 32-33 23.023007147397884 26.469984501239658 27.84092377191265 22.666084579449805 34-35 23.155460396799665 26.39593552934505 28.00797718558956 22.440626888265722 36-37 23.09903266705296 26.278221176422306 27.90290910578727 22.719837050737464 38-39 23.051496451225972 26.426904714772963 27.965799583421653 22.55579925057941 40-41 23.071673435513006 26.153557079432392 28.131232775982845 22.643536709071757 42-43 22.891399584100927 26.223672959214383 27.877320410603033 23.007607046081652 44-45 22.81789630730179 26.561497946248352 27.821187353347593 22.799418393102265 46-47 22.893914087328355 26.369585028995736 27.55866163835341 23.1778392453225 48-49 23.072828815452663 26.419289745656933 27.496300002527985 23.011581436362423 50-51 22.95157277723828 26.582494468749683 27.37835629261065 23.087576461401383 52-53 22.909471964220412 26.758035476719517 27.20170097220939 23.13079158685068 54-55 22.808863192045045 26.650192077706535 27.355096425523918 23.1858483047245 56-57 22.995099637533233 26.640346857838182 27.140463270933285 23.2240902336953 58-59 22.811552752709037 26.57491022023612 27.422254744585604 23.19128228246924 60-61 22.956345463503908 26.728220048020145 27.259814019692953 23.055620468782994 62-63 22.898181464370598 26.664049108314668 27.256891660928375 23.18087776638636 64-65 22.733071232987452 26.47274394584867 27.317109674816454 23.477075146347424 66-67 22.851688846901226 26.41331538617334 27.15869440229932 23.57630136462611 68-69 22.6159858886573 26.501135377408964 27.299985545760187 23.582893188173554 70-71 22.713584038989463 26.238875779158498 27.357592703091804 23.689947478760235 72-73 22.799094198058103 26.436450036815646 27.152082128645826 23.612373636480424 74-75 22.765178018984095 26.550471930707868 27.100171893861408 23.58417815644663 76-77 22.72527109215469 26.429955273267048 27.043324189101153 23.80144944547711 78-79 22.610828098415414 26.375213302437984 27.126751238828025 23.887207360318584 80-81 22.589369933536585 26.550580439479273 27.054985031433304 23.805064595550842 82-83 22.845967550243138 26.351898531581337 27.261660006540932 23.540473911634592 84-85 22.869440207741825 26.409410954939734 26.945428663053082 23.775720174265356 86-87 22.55809413446028 26.575834291713964 27.145043555740212 23.721028018085548 88-89 22.66942027848249 26.160776538055895 27.175659333088287 23.994143850373327 90-91 22.827284814263972 26.102576695532193 27.161982761285724 23.908155728918107 92-93 22.78033814639242 26.180708348971223 27.102541182799555 23.9364123218368 94-95 22.52137298553263 26.018944149699674 27.248842503278365 24.21084036148933 96-97 22.58775794557741 25.971747083288065 27.165135736060787 24.27535923507374 98-99 22.483890226784524 26.030033773302346 27.035496923062574 24.450579076850552 100 22.78674112303209 25.84960031258649 26.740133675809236 24.623524888572184 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1887.0 1 1408.0 2 1912.0 3 2526.5 4 1718.5 5 1446.5 6 2060.0 7 3607.0 8 5775.0 9 6994.0 10 6367.0 11 5499.5 12 5672.5 13 6221.5 14 6853.5 15 7432.5 16 8060.5 17 8595.0 18 9175.5 19 9907.0 20 10561.5 21 11582.0 22 13027.5 23 15201.0 24 18071.5 25 21402.5 26 26050.5 27 32576.5 28 40185.0 29 50074.5 30 63540.0 31 77413.5 32 95309.0 33 116659.5 34 136355.5 35 154661.5 36 176537.0 37 200927.0 38 216180.0 39 223551.5 40 227531.5 41 231339.0 42 239754.0 43 253350.5 44 267177.0 45 284303.5 46 300443.0 47 311470.0 48 322811.5 49 329507.0 50 328896.5 51 326243.5 52 321818.5 53 314958.0 54 303715.0 55 283234.0 56 258696.5 57 229715.0 58 201193.0 59 177249.0 60 149407.5 61 121076.0 62 98075.5 63 77965.0 64 59095.0 65 43365.5 66 32191.0 67 24445.0 68 18702.5 69 14122.5 70 10771.5 71 8199.5 72 6203.0 73 4645.5 74 3478.0 75 2464.0 76 1749.0 77 1197.5 78 786.5 79 521.0 80 380.5 81 272.5 82 180.0 83 146.0 84 109.0 85 87.5 86 72.0 87 56.0 88 49.0 89 35.0 90 26.0 91 23.0 92 18.0 93 12.5 94 13.5 95 12.0 96 6.0 97 4.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024826728818954362 2 0.10881236308559342 3 0.0167721125169863 4 0.01747251393454874 5 0.01952368951455303 6 0.04515087709643589 7 0.04601387170021819 8 0.0386471496476418 9 0.032656216093848785 10-11 0.03677107442202813 12-13 0.03622701260660016 14-15 0.031536824542565955 16-17 0.038071819911786944 18-19 0.03732138982154147 20-21 0.04765856431467285 22-23 0.04697067006528117 24-25 0.06668822068648093 26-27 0.058433489693780746 28-29 0.05066028467565473 30-31 0.04912190299065152 32-33 0.04359373465917654 34-35 0.045325977450826505 36-37 0.04044818186423094 38-39 0.04553859930972939 40-41 0.05494398977413931 42-43 0.056707500486216166 44-45 0.05996561779469859 46-47 0.06164783191366552 48-49 0.060897401823420044 50-51 0.05571943420072629 52-53 0.06454949492928135 54-55 0.05150451852718089 56-57 0.05597583114822683 58-59 0.05418730609980845 60-61 0.04974726139918941 62-63 0.056376060529691074 64-65 0.05413102384304005 66-67 0.05226120220151174 68-69 0.058533547039146816 70-71 0.05932149863390456 72-73 0.06161656399323863 74-75 0.05186097282004749 76-77 0.052792756848768954 78-79 0.06253584085378933 80-81 0.06258586952647235 82-83 0.052523852733097655 84-85 0.059127637527257804 86-87 0.056344792609264185 88-89 0.06481214546086726 90-91 0.07014019910161011 92-93 0.07511179844948636 94-95 0.07878890589168917 96-97 0.07428007176613097 98-99 0.08390433767352914 100 0.13038722818015075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7995415.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.021472809984495 #Duplication Level Percentage of deduplicated Percentage of total 1 74.06699345874812 42.974760470840536 2 14.753364022098012 17.12023818927927 3 5.07125752213613 8.827254912991524 4 2.258355037871291 5.241323417005625 5 1.138983184860198 3.3042740945697764 6 0.6894650626354754 2.4002267031083093 7 0.44404800773957404 1.8035023585172636 8 0.2995729921631463 1.3905332975519757 9 0.2195322159134712 1.1463824246885204 >10 0.9884142993188352 9.786864701361509 >50 0.0454883608088072 1.7865616739885022 >100 0.022786203411927747 2.3021914947751507 >500 0.0011597678951962792 0.43273195538600373 >1k 4.928847399292862E-4 0.5282200080023617 >5k 0.0 0.0 >10k+ 8.697965988988771E-5 0.9549342979337038 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25400 0.31768207153724975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19729 0.24675392084088194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11637 0.1455459160031093 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2507168170757865E-5 2 0.0 0.0 0.0 0.0 1.2507168170757865E-5 3 0.0 0.0 0.0 0.0 1.2507168170757865E-5 4 0.0 0.0 0.0 0.0 1.2507168170757865E-5 5 0.0 0.0 0.0 0.0 1.2507168170757865E-5 6 5.002867268303146E-5 0.0 0.0 0.0 3.7521504512273594E-5 7 5.002867268303146E-5 0.0 0.0 0.0 3.7521504512273594E-5 8 5.002867268303146E-5 0.0 0.0 0.0 5.002867268303146E-5 9 5.002867268303146E-5 0.0 0.0 1.2507168170757865E-5 6.253584085378933E-5 10-11 5.002867268303146E-5 0.0 0.0 1.2507168170757865E-5 6.253584085378933E-5 12-13 5.002867268303146E-5 0.0 0.0 1.2507168170757865E-5 6.253584085378933E-5 14-15 5.002867268303146E-5 0.0 0.0 1.2507168170757865E-5 8.129659310992612E-5 16-17 8.755017719530506E-5 0.0 0.0 2.501433634151573E-5 1.3757884987833653E-4 18-19 8.755017719530506E-5 0.0 0.0 3.1267920426894665E-5 1.3757884987833653E-4 20-21 8.755017719530506E-5 0.0 0.0 1.0631092945144187E-4 1.500860180490944E-4 22-23 8.755017719530506E-5 0.0 0.0 4.1273654963500957E-4 1.8135393847598904E-4 24-25 8.755017719530506E-5 0.0 0.0 0.001025587790002145 2.1887544298826266E-4 26-27 8.755017719530506E-5 0.0 0.0 0.002251290270736416 2.2512902707364158E-4 28-29 8.755017719530506E-5 0.0 0.0 0.005772058110804754 2.4388977932977837E-4 30-31 8.755017719530506E-5 0.0 0.0 0.018035336502232845 2.689041156712941E-4 32-33 9.3803761280684E-5 0.0 0.0 0.04491949448527688 2.876648679274309E-4 34-35 1.0005734536606292E-4 6.253584085378932E-6 0.0 0.08768775604518339 3.0017203609818875E-4 36-37 1.0005734536606292E-4 3.7521504512273594E-5 0.0 0.15662726700240076 3.0017203609818875E-4 38-39 1.0005734536606292E-4 3.7521504512273594E-5 0.0 0.2547460013019962 3.251863724397045E-4 40-41 1.0005734536606292E-4 3.7521504512273594E-5 0.0 0.36149468163941456 3.251863724397045E-4 42-43 1.0005734536606292E-4 3.7521504512273594E-5 0.0 0.4705884560088501 3.251863724397045E-4 44-45 1.0005734536606292E-4 3.7521504512273594E-5 0.0 0.6016198033497948 3.314399565250834E-4 46-47 1.0005734536606292E-4 3.7521504512273594E-5 0.0 0.7547012881757857 3.627078769519781E-4 48-49 1.0005734536606292E-4 3.7521504512273594E-5 0.0 0.9203712377656443 4.0022938146425167E-4 50-51 1.0005734536606292E-4 3.7521504512273594E-5 0.0 1.0913629874121606 4.064829655496306E-4 52-53 1.0005734536606292E-4 4.377508859765252E-5 0.0 1.2566076932842134 4.690188064034199E-4 54-55 1.0005734536606292E-4 6.878942493916825E-5 0.0 1.443233403144177 5.50315399513346E-4 56-57 1.1256451353682079E-4 7.504300902454719E-5 0.0 1.6556801616926702 5.815833199402407E-4 58-59 1.1256451353682079E-4 7.504300902454719E-5 0.0 1.8950611068968901 5.878369040256197E-4 60-61 1.5633960213447333E-4 7.504300902454719E-5 0.0 2.137718930161849 5.878369040256197E-4 62-63 2.2512902707364158E-4 7.504300902454719E-5 0.0 2.3847917837910853 6.50372744879409E-4 64-65 2.2512902707364158E-4 8.755017719530506E-5 0.0 2.636542318316185 6.50372744879409E-4 66-67 2.2512902707364158E-4 8.755017719530506E-5 0.0 2.9216369631845254 6.753870812209247E-4 68-69 2.2512902707364158E-4 8.755017719530506E-5 0.0 3.2355043484296937 6.941478334770616E-4 70-71 2.4388977932977837E-4 8.755017719530506E-5 0.0 3.5693081597390504 7.50430090245472E-4 72-73 3.5020070878122024E-4 8.755017719530506E-5 0.0 3.895463837711989 9.130232764653242E-4 74-75 3.5020070878122024E-4 8.755017719530506E-5 0.0 4.221581994180415 9.130232764653242E-4 76-77 3.5020070878122024E-4 8.755017719530506E-5 0.0 4.579668472493298 9.192768605507031E-4 78-79 3.627078769519781E-4 8.755017719530506E-5 0.0 4.966827863219107 9.25530444636082E-4 80-81 3.627078769519781E-4 8.755017719530506E-5 0.0 5.372854317130505 9.505447809775977E-4 82-83 3.627078769519781E-4 8.755017719530506E-5 0.0 5.784909226100209 9.880662854898714E-4 84-85 3.627078769519781E-4 1.0005734536606292E-4 0.0 6.193931146788503 9.880662854898714E-4 86-87 3.627078769519781E-4 1.0631092945144185E-4 0.0 6.620369299154579 9.943198695752504E-4 88 3.75215045122736E-4 1.1256451353682079E-4 0.0 6.957387452683819 0.0010005734536606293 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12930 0.0 52.235973 1 GTATCAA 22735 0.0 40.279564 1 CAACGCA 27990 0.0 32.063976 5 ATCAACG 28130 0.0 31.905197 3 TATCAAC 28800 0.0 31.420082 2 TCAACGC 29165 0.0 30.820902 4 AACGCAG 29830 0.0 30.432531 6 TACCTGG 6435 0.0 28.211975 2 ACGCAGA 33740 0.0 26.558325 7 GTACATG 30890 0.0 26.235004 1 CGCAGAG 34720 0.0 25.752626 8 GTGGTAT 6050 0.0 25.577276 1 TACATGG 31635 0.0 25.259241 2 ACATGGG 32390 0.0 24.08213 3 GTACTAG 2475 0.0 23.944687 1 GTACCTG 7805 0.0 23.803345 1 TGGTATC 6295 0.0 23.011755 2 GCAGAGT 38010 0.0 22.907518 9 CATGGGG 22250 0.0 22.364454 4 ACCTGGG 8215 0.0 21.449713 3 >>END_MODULE