FastQCFastQC Report
Mon 6 Jun 2016
ERR1211321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211321_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7995415
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226830.2837000956173007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185910.23252076346255948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114280.1429319178554209No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127150.049.3477671
GTATCAA229400.041.3861471
CAACGCA286300.032.6411635
ATCAACG287650.032.4469573
TATCAAC293400.032.111332
TCAACGC297700.031.4305954
AACGCAG308500.030.5812026
ACGCAGA348100.026.7653477
CGCAGAG360150.025.8437718
TACCTGG63650.025.8002742
GTACATG310250.025.6092971
GTGGTAT60650.024.3743041
TACATGG321050.024.2453442
ACATGGG326500.023.1822073
GCAGAGT399600.022.9858429
GTACCTG77300.022.15981
CATGGGG222050.021.5765134
TGGTATC67250.021.279542
GAGTACT219050.020.7374912-13
ATGGGGG143950.020.1081035
Produced by FastQC (version 0.11.5)