##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211321_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7995415 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08515205277024 33.0 31.0 34.0 30.0 34.0 2 32.21800031643136 33.0 31.0 34.0 30.0 34.0 3 32.2847913460402 34.0 31.0 34.0 30.0 34.0 4 35.93085774784674 37.0 35.0 37.0 35.0 37.0 5 35.82996167178314 37.0 35.0 37.0 35.0 37.0 6 35.844786043000894 37.0 35.0 37.0 35.0 37.0 7 35.75714568912308 37.0 35.0 37.0 33.0 37.0 8 35.73777146026816 37.0 35.0 37.0 33.0 37.0 9 37.35139264190789 39.0 37.0 39.0 34.0 39.0 10-11 37.299502214706806 39.0 37.0 39.0 34.0 39.0 12-13 37.22905846663369 39.0 37.0 39.0 33.0 39.0 14-15 38.4603850456793 40.0 38.0 41.0 34.0 41.0 16-17 38.4412689022396 40.0 38.0 41.0 34.0 41.0 18-19 38.43881780995733 40.0 38.0 41.0 34.0 41.0 20-21 38.40473102396811 40.0 38.0 41.0 34.0 41.0 22-23 38.29614835502598 40.0 38.0 41.0 34.0 41.0 24-25 38.20455417261017 40.0 38.0 41.0 33.0 41.0 26-27 38.107958436178734 40.0 37.5 41.0 33.0 41.0 28-29 38.0018867688544 40.0 37.0 41.0 33.0 41.0 30-31 38.01359954423879 40.0 37.0 41.0 33.0 41.0 32-33 37.957464684447274 40.0 37.0 41.0 33.0 41.0 34-35 37.82815263497892 40.0 37.0 41.0 33.0 41.0 36-37 37.68336840551741 40.0 37.0 41.0 33.0 41.0 38-39 37.571107628559616 40.0 37.0 41.0 32.0 41.0 40-41 37.45915409018794 40.0 36.0 41.0 32.0 41.0 42-43 37.355729877686144 40.0 36.0 41.0 31.5 41.0 44-45 37.19993258636356 39.5 36.0 41.0 31.0 41.0 46-47 36.96338732886285 39.0 36.0 41.0 31.0 41.0 48-49 35.41379897103528 39.0 35.0 40.5 24.0 41.0 50-51 36.143410317037954 39.0 35.0 40.0 29.0 41.0 52-53 36.23829970051586 39.0 35.0 40.0 29.5 41.0 54-55 36.064673378430015 38.0 35.0 40.0 29.0 41.0 56-57 35.844391191701746 38.0 34.5 40.0 28.5 41.0 58-59 35.59830559139207 38.0 34.0 40.0 28.0 41.0 60-61 35.33536833297583 37.5 34.0 40.0 28.0 41.0 62-63 35.00822621464927 37.0 34.0 40.0 27.5 41.0 64-65 34.663122364505156 36.0 33.5 39.0 26.5 41.0 66-67 34.3197527582996 36.0 33.0 39.0 26.0 40.0 68-69 33.95178111455128 35.0 33.0 38.5 26.0 40.0 70-71 33.586453611225934 35.0 32.5 38.0 26.0 40.0 72-73 33.2042295490603 35.0 32.0 37.0 25.5 39.5 74-75 32.84298957590069 35.0 32.0 37.0 25.0 39.0 76-77 31.918866687970542 34.0 30.5 36.0 24.5 38.0 78-79 32.3650095085746 34.5 31.5 36.0 25.0 38.0 80-81 32.244857896682035 34.5 31.5 35.5 25.0 37.0 82-83 31.98297411704083 34.0 31.0 35.0 25.0 37.0 84-85 31.707054605670876 34.0 31.0 35.0 24.0 36.0 86-87 31.453701277544695 34.0 31.0 35.0 24.0 36.0 88-89 31.234628784121902 34.0 31.0 35.0 23.0 35.5 90-91 31.01942669642539 34.0 30.5 35.0 23.0 35.0 92-93 30.83135984811295 34.0 30.0 35.0 21.0 35.0 94-95 30.641816591133797 34.0 30.0 35.0 20.0 35.0 96-97 30.4547651372693 34.0 29.5 35.0 20.0 35.0 98-99 30.23733214598617 34.0 29.0 35.0 19.0 35.0 100 27.77540853101434 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2681250500350636 1101 2 -0.25543055780489965 1101 3 -0.2683030429987774 1101 4 -0.12551415130763388 1101 5 -0.09790691572684551 1101 6 -0.20999091244861745 1101 7 -0.22430508230111457 1101 8 -0.241202545401336 1101 9 -0.342643476986602 1101 10-11 -0.28665480230516494 1101 12-13 -0.33518554570147785 1101 14-15 -0.487630805535737 1101 16-17 -0.47719779283513475 1101 18-19 -0.4991754106898867 1101 20-21 -0.5783981916021901 1101 22-23 -0.5819531856176923 1101 24-25 -0.5250928147206864 1101 26-27 -0.5911116228812787 1101 28-29 -0.6519790427512362 1101 30-31 -0.5592375206501075 1101 32-33 -0.5166696219375666 1101 34-35 -0.5736796905504633 1101 36-37 -0.538852122986718 1101 38-39 -0.5740383081050666 1101 40-41 -0.5857729999316703 1101 42-43 -0.5389419621362279 1101 44-45 -0.5888384172785734 1101 46-47 -0.6375694706719344 1101 48-49 0.5283837464667727 1101 50-51 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1105 68-69 -0.7929589137835151 1105 70-71 -0.8113627375812555 1105 72-73 -0.6673616943275036 1105 74-75 -0.7942034522840231 1105 76-77 -0.7647375692526275 1105 78-79 -0.7125423626853973 1105 80-81 -0.6370925407181147 1105 82-83 -0.6255184622886922 1105 84-85 -0.6752460136543377 1105 86-87 -0.677546043614953 1105 88-89 -0.7436496657208558 1105 90-91 -0.739263926249091 1105 92-93 -0.7401487041783916 1105 94-95 -0.724310080790243 1105 96-97 -0.7298926131703247 1105 98-99 -0.6991988602203669 1105 100 -0.882173333502724 1106 1 -0.31164666039546773 1106 2 -0.28144036572623676 1106 3 -0.3520147084484222 1106 4 -0.20620890368418543 1106 5 -0.220171591500943 1106 6 -0.33600438390688936 1106 7 -0.33702883377827675 1106 8 -0.3519982023262571 1106 9 -0.49526034525329266 1106 10-11 -0.4632745699448648 1106 12-13 -0.42748859846350484 1106 14-15 -0.5991449280869929 1106 16-17 -0.571100228818672 1106 18-19 -0.6061974711282687 1106 20-21 -0.6363341318777103 1106 22-23 -0.6122415251550635 1106 24-25 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0.5393842725251545 2115 28-29 0.5754942752101044 2115 30-31 0.5966102094365837 2115 32-33 0.6070948600929427 2115 34-35 0.6089381033217478 2115 36-37 0.5245295993762298 2115 38-39 0.6276466039874862 2115 40-41 0.6624254569194079 2115 42-43 0.586924668711049 2115 44-45 0.6050774482608503 2115 46-47 0.7198743996427694 2115 48-49 1.8487132710928407 2115 50-51 0.9316067299365258 2115 52-53 0.7920078945847919 2115 54-55 -0.3610020538980976 2115 56-57 0.3934769292503546 2115 58-59 0.6448437054811933 2115 60-61 0.7047824174081754 2115 62-63 0.7137822245341994 2115 64-65 0.6924240707429732 2115 66-67 0.7887268861614274 2115 68-69 0.5555922843193244 2115 70-71 0.6502793004132528 2115 72-73 0.7339153289519444 2115 74-75 0.8197902017811387 2115 76-77 0.535829649799151 2115 78-79 0.5120913639916651 2115 80-81 0.48241161137133304 2115 82-83 -0.3002374302466144 2115 84-85 0.5148562758826998 2115 86-87 0.679979918024955 2115 88-89 0.7620956656543321 2115 90-91 0.7804534007205568 2115 92-93 0.8170014919180986 2115 94-95 0.8230628283393777 2115 96-97 0.7287128358469595 2115 98-99 0.7783679830629424 2115 100 0.808759982456241 2116 1 0.1093832216335997 2116 2 -0.09484047353816294 2116 3 -0.001942103364974912 2116 4 -0.06404708982744012 2116 5 0.07455144537528469 2116 6 0.016835720351359384 2116 7 0.034777953419528274 2116 8 0.058142511598141766 2116 9 0.2653107301955515 2116 10-11 0.3254061636894363 2116 12-13 0.2073730543872756 2116 14-15 0.4416487095111208 2116 16-17 0.5006537054676699 2116 18-19 0.51309231388786 2116 20-21 0.572412079446579 2116 22-23 0.5842879881939922 2116 24-25 0.41919161137115424 2116 26-27 0.5882134227155404 2116 28-29 0.441736402614076 2116 30-31 0.45218789992186714 2116 32-33 0.5050395155367511 2116 34-35 0.5245233247439103 2116 36-37 0.3349737807585882 2116 38-39 0.3475078262775284 2116 40-41 0.31072033464896975 2116 42-43 0.435986162138839 2116 44-45 0.3262531604050949 2116 46-47 0.44743210499036934 2116 48-49 1.7873706133266936 2116 50-51 0.3423481692180701 2116 52-53 0.19163619575593316 2116 54-55 0.43838754549144454 2116 56-57 0.4494655161139107 2116 58-59 0.6229238196273386 2116 60-61 0.6824211982361987 2116 62-63 0.6364943739082918 2116 64-65 0.4854371566407778 2116 66-67 0.668132776404498 2116 68-69 0.6851707017527318 2116 70-71 0.692415222483227 2116 72-73 0.5861854273908946 2116 74-75 0.6794100601544386 2116 76-77 0.5924133830032297 2116 78-79 0.5305190278948615 2116 80-81 0.5188213849226315 2116 82-83 0.5147643179488419 2116 84-85 0.49789904553428244 2116 86-87 -0.017739192719361085 2116 88-89 0.004461128167168482 2116 90-91 0.3531000222133045 2116 92-93 0.28482160535378753 2116 94-95 0.3663959374799468 2116 96-97 0.457625450417968 2116 98-99 0.4972750310784271 2116 100 0.23658340358213792 2201 1 0.47042774487302097 2201 2 0.5008795157878794 2201 3 0.45134792081222486 2201 4 0.3031558406872179 2201 5 0.33571791612966706 2201 6 0.3631214819258872 2201 7 0.4027336983770198 2201 8 0.3644144464205681 2201 9 0.5651820452621337 2201 10-11 0.5750747026796432 2201 12-13 0.5375054580194387 2201 14-15 0.683908940578327 2201 16-17 0.6716634073984054 2201 18-19 0.6688840313258737 2201 20-21 0.61360934735238 2201 22-23 0.6728839397926052 2201 24-25 0.6971733150074542 2201 26-27 0.6429928171528019 2201 28-29 0.6202249098394859 2201 30-31 0.7426509721529229 2201 32-33 0.7825938059147219 2201 34-35 0.7952891364588908 2201 36-37 0.818809019272102 2201 38-39 0.8261457205326437 2201 40-41 0.8308806392332357 2201 42-43 0.8644140530582263 2201 44-45 0.8669794408768681 2201 46-47 0.9110747539517092 2201 48-49 2.1638281607783156 2201 50-51 1.3736376949851703 2201 52-53 1.0553746754082312 2201 54-55 1.0243252376817793 2201 56-57 0.920291507249793 2201 58-59 0.9141330085995776 2201 60-61 0.9137282980984125 2201 62-63 0.9626224305455509 2201 64-65 0.9682995494781608 2201 66-67 0.9415275413220314 2201 68-69 0.9469755723935762 2201 70-71 0.8973490239167035 2201 72-73 0.910453147752257 2201 74-75 0.8910858956316403 2201 76-77 0.9790767774845897 2201 78-79 0.7731056771381191 2201 80-81 0.7106475435431747 2201 82-83 0.7322614249990842 2201 84-85 0.20295002966675924 2201 86-87 -0.011472851952145646 2201 88-89 0.21607765317954986 2201 90-91 0.42016190021466215 2201 92-93 0.5611402871247897 2201 94-95 0.6357869872612092 2201 96-97 0.6535089479117886 2201 98-99 0.7008355509327266 2201 100 1.0743071074186048 2202 1 0.5746582930906285 2202 2 0.5456768548724966 2202 3 0.5140826769142492 2202 4 0.3407678541014434 2202 5 0.4208040024748314 2202 6 0.44271276418508165 2202 7 0.5133154994996616 2202 8 0.38153715043931413 2202 9 0.5309196374816381 2202 10-11 0.5730306129438247 2202 12-13 0.6242187049915628 2202 14-15 0.7819486985726698 2202 16-17 0.7408882413297206 2202 18-19 0.7374874217940004 2202 20-21 0.763461349886768 2202 22-23 0.7506971965935278 2202 24-25 0.702462311143222 2202 26-27 0.6605069506659333 2202 28-29 0.7150626583354693 2202 30-31 0.8265609842215937 2202 32-33 0.8659343265929991 2202 34-35 0.9058796835517597 2202 36-37 0.9098744970320283 2202 38-39 0.904083989177856 2202 40-41 0.8821959662671475 2202 42-43 0.8978776496041192 2202 44-45 0.8455357669191699 2202 46-47 0.8943254132565173 2202 48-49 2.133543181731035 2202 50-51 1.29494732969237 2202 52-53 0.7592587014703653 2202 54-55 0.7389557326814824 2202 56-57 0.6749707275262296 2202 58-59 0.7170920732184385 2202 60-61 0.8615015601958831 2202 62-63 0.9333931393245294 2202 64-65 0.9284616298489041 2202 66-67 0.9180447248439805 2202 68-69 0.875709412604472 2202 70-71 0.892434148331354 2202 72-73 0.9269978415784337 2202 74-75 0.9168463621601219 2202 76-77 0.9640892260417537 2202 78-79 0.8067452405857267 2202 80-81 0.8159279615606678 2202 82-83 0.7600732064696665 2202 84-85 0.8202311430204361 2202 86-87 0.8354503687275354 2202 88-89 0.7814434571073683 2202 90-91 0.8916373789625105 2202 92-93 0.8246829866173435 2202 94-95 0.8490762568063595 2202 96-97 0.8020165572592575 2202 98-99 0.1530251556921094 2202 100 0.6484925218864745 2203 1 0.5833783929812668 2203 2 0.5716062188426676 2203 3 0.5383541751711363 2203 4 0.3435133796656089 2203 5 0.41867670421419945 2203 6 0.4386867758919948 2203 7 0.4679605001468303 2203 8 0.4819128071894525 2203 9 0.5963056669637083 2203 10-11 0.5666897446014261 2203 12-13 0.652846208422126 2203 14-15 0.7478419134814587 2203 16-17 0.7727208268353039 2203 18-19 0.7514957869604615 2203 20-21 0.7185089028137668 2203 22-23 0.7026679615628808 2203 24-25 0.7049261809532581 2203 26-27 0.7480405203833556 2203 28-29 0.6886215772846356 2203 30-31 0.7371152565773755 2203 32-33 0.7855041684636888 2203 34-35 0.8843146847887198 2203 36-37 0.8833374674559522 2203 38-39 0.8633432753696368 2203 40-41 0.8914448367001953 2203 42-43 0.9373412031796917 2203 44-45 0.9420652547260246 2203 46-47 0.9468696576483921 2203 48-49 2.122598413408504 2203 50-51 1.2875792151643637 2203 52-53 1.0087852241513815 2203 54-55 0.9451764580963626 2203 56-57 0.9115537733935071 2203 58-59 1.0131413139034677 2203 60-61 0.9787223320595899 2203 62-63 1.000150189128739 2203 64-65 0.9723346512950783 2203 66-67 0.9426316146408027 2203 68-69 0.9657208924589682 2203 70-71 0.9178192986574558 2203 72-73 0.9442191267444215 2203 74-75 0.9546167101669809 2203 76-77 0.9827122038572291 2203 78-79 0.8199094122175055 2203 80-81 0.7715550365348065 2203 82-83 0.6920646741277032 2203 84-85 0.7664629872168973 2203 86-87 0.823976509048677 2203 88-89 0.837999630593874 2203 90-91 0.8602736598620488 2203 92-93 0.8584021341309551 2203 94-95 0.872950222577753 2203 96-97 0.977216463335008 2203 98-99 0.9611485618130473 2203 100 1.1789544078838503 2204 1 0.5866516006568716 2204 2 0.5262543055731825 2204 3 0.527575180906986 2204 4 0.36427334030801717 2204 5 0.4142420341396331 2204 6 0.44716027303685024 2204 7 0.4762978716414139 2204 8 0.45664397151418257 2204 9 0.6587298141160289 2204 10-11 0.4420282110935432 2204 12-13 0.6205077025804115 2204 14-15 0.7811249345880285 2204 16-17 0.7976854771112372 2204 18-19 0.7772119746672388 2204 20-21 0.7863142577152047 2204 22-23 0.7678941948033469 2204 24-25 0.7966822847355317 2204 26-27 0.7967120568794073 2204 28-29 0.7930124734248523 2204 30-31 0.8623613913279868 2204 32-33 0.8426541457727552 2204 34-35 0.8755696354479099 2204 36-37 0.8932670746151032 2204 38-39 0.9507780389244758 2204 40-41 0.9534778098681613 2204 42-43 1.019945928048081 2204 44-45 1.061105887483592 2204 46-47 1.0937535038182133 2204 48-49 2.3258490150379387 2204 50-51 1.4516449297970624 2204 52-53 1.1897704432827183 2204 54-55 1.1661764013690217 2204 56-57 1.1302783809161951 2204 58-59 1.1317971894580552 2204 60-61 1.0414410269551624 2204 62-63 1.0871196985810343 2204 64-65 1.1429294784404718 2204 66-67 1.0954107850793093 2204 68-69 1.0322668603832525 2204 70-71 1.0975717217539582 2204 72-73 1.1308724861217314 2204 74-75 1.238158824427039 2204 76-77 1.230658359421188 2204 78-79 1.0578649138110663 2204 80-81 0.9987395835669659 2204 82-83 1.0200302408298647 2204 84-85 1.050874787582984 2204 86-87 1.0438386845742684 2204 88-89 0.9076204602352682 2204 90-91 0.3013045624819739 2204 92-93 0.8546242977837473 2204 94-95 1.0160808009329934 2204 96-97 1.0689192104564924 2204 98-99 1.1085015321286669 2204 100 1.440201552540973 2205 1 0.4792700204564113 2205 2 0.48297666530215366 2205 3 0.5169886308174725 2205 4 0.3407167102986506 2205 5 0.04351961491350664 2205 6 0.3004394446607819 2205 7 0.40774851605748097 2205 8 0.4391357362496606 2205 9 0.5956750106493018 2205 10-11 0.6213586205477597 2205 12-13 0.6122366249651776 2205 14-15 0.7686131374805072 2205 16-17 0.7596856386320283 2205 18-19 0.726455337590096 2205 20-21 0.7754774901516157 2205 22-23 0.7635443639210067 2205 24-25 0.7591940868344267 2205 26-27 0.7938385490250894 2205 28-29 0.7969670126084196 2205 30-31 0.8230824888388284 2205 32-33 0.8710851249820877 2205 34-35 0.8761444917513046 2205 36-37 0.9009924205820639 2205 38-39 0.9101718308158198 2205 40-41 0.8901993929396639 2205 42-43 0.8990200854595827 2205 44-45 0.9447149308911591 2205 46-47 0.9357681722623425 2205 48-49 2.188006414756778 2205 50-51 1.337753277906522 2205 52-53 1.079672708227534 2205 54-55 1.0447806710284837 2205 56-57 1.0247968662624203 2205 58-59 0.9798818153736946 2205 60-61 0.9549259020001628 2205 62-63 1.0043186784244753 2205 64-65 1.0067314353777093 2205 66-67 1.022176028679219 2205 68-69 1.0221761781365828 2205 70-71 1.0580503633740506 2205 72-73 1.0433722889625372 2205 74-75 1.028502985510194 2205 76-77 1.0607410285193843 2205 78-79 0.9371471365608173 2205 80-81 0.827625845774115 2205 82-83 0.893180737880666 2205 84-85 0.9862771760143403 2205 86-87 1.0289044498923374 2205 88-89 1.0300546205457586 2205 90-91 1.0000021937565542 2205 92-93 0.9929635146135389 2205 94-95 1.0118966336845006 2205 96-97 1.0476899323865645 2205 98-99 1.1246559825456792 2205 100 1.3859924583811392 2206 1 0.5252720010531462 2206 2 0.5295612376692844 2206 3 0.4856144588626705 2206 4 0.3372223627241837 2206 5 0.3833160972014795 2206 6 0.41102154254835455 2206 7 0.462533961964013 2206 8 0.43249463329535587 2206 9 0.6269439222455446 2206 10-11 0.5965884660130882 2206 12-13 0.584448808421115 2206 14-15 0.7909028605388855 2206 16-17 0.7466592070367 2206 18-19 0.7109538882339734 2206 20-21 0.7394484093182001 2206 22-23 0.7245823029860006 2206 24-25 0.7602769629040367 2206 26-27 0.7893070791918291 2206 28-29 0.8286351841402606 2206 30-31 0.8198081597279767 2206 32-33 0.8569452599007477 2206 34-35 0.8929894606683462 2206 36-37 0.9246630779907505 2206 38-39 0.9729152928837195 2206 40-41 0.9692964928344239 2206 42-43 0.9580433562048754 2206 44-45 0.9573795046766165 2206 46-47 0.9957363674752173 2206 48-49 2.228810177384041 2206 50-51 1.419181365402288 2206 52-53 1.1364100486812205 2206 54-55 1.0979027702584077 2206 56-57 1.064001246369834 2206 58-59 1.0824504682513236 2206 60-61 1.0575530483325792 2206 62-63 1.095004086466659 2206 64-65 1.0670624571706 2206 66-67 1.0995623058285275 2206 68-69 1.1293819221203947 2206 70-71 1.1268215512635393 2206 72-73 1.0968826687330946 2206 74-75 1.1340786755847958 2206 76-77 1.1015357492919655 2206 78-79 0.9889282299667244 2206 80-81 0.9764170612450584 2206 82-83 0.9245066549514114 2206 84-85 1.0424038936817936 2206 86-87 1.052468561580774 2206 88-89 1.0098705098957446 2206 90-91 0.9973807524038705 2206 92-93 1.05474568443071 2206 94-95 1.042332491603279 2206 96-97 1.148633020623162 2206 98-99 1.1865830183243204 2206 100 1.3136682711666232 2207 1 0.5416559994767738 2207 2 0.501866345440213 2207 3 0.4894791876093407 2207 4 0.3262448440402821 2207 5 0.36526983355623344 2207 6 0.38451215109721204 2207 7 0.4204469980832286 2207 8 0.4438800006247092 2207 9 0.5763980746402808 2207 10-11 0.5930603207114089 2207 12-13 0.5788546543906392 2207 14-15 0.7396695980560253 2207 16-17 0.6878013828168292 2207 18-19 0.6788959539963955 2207 20-21 0.6688874232260957 2207 22-23 0.7153197089743841 2207 24-25 0.7716386151653509 2207 26-27 0.7369386512418998 2207 28-29 0.758842951503965 2207 30-31 0.8105000365750925 2207 32-33 0.813664818642593 2207 34-35 0.7991406251223978 2207 36-37 0.8294796493603158 2207 38-39 0.84668102148418 2207 40-41 0.8607098508912188 2207 42-43 0.898877577142791 2207 44-45 0.8590240988568567 2207 46-47 0.9042859305464077 2207 48-49 2.1535261665502574 2207 50-51 1.2979557858436053 2207 52-53 1.0722991190031834 2207 54-55 1.0398977664106326 2207 56-57 0.9835936314014901 2207 58-59 0.9780041550917957 2207 60-61 0.9434107080311449 2207 62-63 0.9243529073288457 2207 64-65 0.9534996676596776 2207 66-67 1.0388336058910852 2207 68-69 1.0089120416778101 2207 70-71 0.9971238441727337 2207 72-73 1.0186954220082853 2207 74-75 0.9934258891274581 2207 76-77 1.0341822719398586 2207 78-79 0.9217839315143266 2207 80-81 0.855491699921842 2207 82-83 0.8686569308009133 2207 84-85 0.8896022347006891 2207 86-87 0.9516175411539223 2207 88-89 0.9761173809119832 2207 90-91 1.0060588252498661 2207 92-93 0.9999691634217207 2207 94-95 0.9861454387673056 2207 96-97 1.003414998308262 2207 98-99 1.055960701466379 2207 100 1.232559492265267 2208 1 0.5281612564690192 2208 2 0.533864356321331 2208 3 0.5309877778688872 2208 4 0.33813765741939505 2208 5 0.4132007413906038 2208 6 0.46366852696636585 2208 7 0.48949037697943254 2208 8 0.47804454901084625 2208 9 0.6202091892633774 2208 10-11 0.6621367661711446 2208 12-13 0.6562460346717955 2208 14-15 0.8536535537257421 2208 16-17 0.8545499089400508 2208 18-19 0.809894276669084 2208 20-21 0.770357698898728 2208 22-23 0.8052070039826376 2208 24-25 0.8126648820409983 2208 26-27 0.884404331992755 2208 28-29 0.9286026600884867 2208 30-31 0.9297108192611461 2208 32-33 0.9126304253263129 2208 34-35 0.9431918875111478 2208 36-37 0.9833473630001279 2208 38-39 0.9471264061656015 2208 40-41 0.9659088066383035 2208 42-43 0.9900234097061329 2208 44-45 1.0229054249807987 2208 46-47 0.9960807128989373 2208 48-49 2.2789358026298103 2208 50-51 1.469044218351499 2208 52-53 1.2227917886240647 2208 54-55 1.1923825732856415 2208 56-57 1.2340315662502093 2208 58-59 1.1848637040500734 2208 60-61 1.1255291556156166 2208 62-63 1.1655227242386417 2208 64-65 1.174682465536435 2208 66-67 1.106186968001694 2208 68-69 1.1267188259609142 2208 70-71 1.11404793970366 2208 72-73 1.2172943791450521 2208 74-75 1.189143034884772 2208 76-77 1.1752685699049827 2208 78-79 1.100537406583463 2208 80-81 1.0449040228198356 2208 82-83 1.0442695812895018 2208 84-85 1.088062389885863 2208 86-87 1.1542788500517034 2208 88-89 1.1860002166157138 2208 90-91 1.1360875362422895 2208 92-93 1.198470220615441 2208 94-95 1.227515281282745 2208 96-97 1.23075444166248 2208 98-99 1.323181543533277 2208 100 1.4773616108119043 2209 1 0.3786983736786027 2209 2 0.41819413127874583 2209 3 0.4163290037137344 2209 4 0.27363103575643066 2209 5 0.3819137151280074 2209 6 0.4149381468200346 2209 7 0.452934653583533 2209 8 0.5059538887237238 2209 9 0.6040016060920621 2209 10-11 0.6130224572597882 2209 12-13 0.6509518844297872 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0.8749662040536776 2209 82-83 0.9230317145804356 2209 84-85 0.997410956537351 2209 86-87 1.101308786665875 2209 88-89 1.075434496279481 2209 90-91 1.1061783687866047 2209 92-93 1.0763655870173388 2209 94-95 1.055390669463737 2209 96-97 1.0915983393007487 2209 98-99 1.2265266698592363 2209 100 1.4782777299897916 2210 1 0.5656420766095209 2210 2 0.4964944682107273 2210 3 0.465483591008514 2210 4 0.3227030906367929 2210 5 0.36830812639569643 2210 6 0.4265401522839056 2210 7 0.45678146870896086 2210 8 0.506051549303514 2210 9 0.6236076489073881 2210 10-11 0.6252848827236619 2210 12-13 0.650313180667176 2210 14-15 0.7827844254711707 2210 16-17 0.7950513378884665 2210 18-19 0.8044691017534973 2210 20-21 0.793309543124721 2210 22-23 0.8261608164284056 2210 24-25 0.8144463362622716 2210 26-27 0.8830891700766514 2210 28-29 0.8566396365305877 2210 30-31 0.8947272250353748 2210 32-33 0.9029088580830376 2210 34-35 0.8963473648522324 2210 36-37 0.910884668557074 2210 38-39 0.8961915802237641 2210 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4 0.33687709818791944 2211 5 0.3881129937441088 2211 6 0.42145398760611386 2211 7 0.45607193646520017 2211 8 0.46846250505704745 2211 9 0.5873885606833227 2211 10-11 0.5759890409226855 2211 12-13 0.575384167567961 2211 14-15 0.7158561928136962 2211 16-17 0.7001071748986547 2211 18-19 0.7471823697382334 2211 20-21 0.7353062954434293 2211 22-23 0.7104089914687464 2211 24-25 0.7259602672916188 2211 26-27 0.816440936021074 2211 28-29 0.8431210544711121 2211 30-31 0.8322777942882738 2211 32-33 0.8920547765610465 2211 34-35 0.8827768029712786 2211 36-37 0.8563141805751471 2211 38-39 0.8630059677373367 2211 40-41 0.9015351643016132 2211 42-43 0.8388394263686152 2211 44-45 0.8164802107156177 2211 46-47 0.9397782491914697 2211 48-49 2.194814849980439 2211 50-51 1.3174520510822134 2211 52-53 1.091553018973876 2211 54-55 1.060547378902406 2211 56-57 1.0481897858186997 2211 58-59 1.119839380674172 2211 60-61 1.0558897217161274 2211 62-63 1.1045998044998058 2211 64-65 1.1441903736352188 2211 66-67 1.1309160284876754 2211 68-69 1.1620865351905465 2211 70-71 1.0912042529707975 2211 72-73 1.1049879989382916 2211 74-75 1.1464055206994388 2211 76-77 0.9344859832589627 2211 78-79 0.7854434122267904 2211 80-81 0.8584960969074729 2211 82-83 0.9375415314512914 2211 84-85 0.995266646737349 2211 86-87 1.031457951684942 2211 88-89 1.0597156803302603 2211 90-91 1.0502134603334916 2211 92-93 1.0371366965180364 2211 94-95 1.0234901760994184 2211 96-97 1.1728934574882466 2211 98-99 1.16711565262775 2211 100 1.4217533097238224 2212 1 0.5222681230760955 2212 2 0.4966593420978569 2212 3 0.5015106591498721 2212 4 0.32263332168965064 2212 5 0.3748335546675037 2212 6 0.2739572491108788 2212 7 0.25087868656255097 2212 8 0.33408703751273094 2212 9 0.5020132089480001 2212 10-11 0.5136083270962928 2212 12-13 0.5894862907549197 2212 14-15 0.7188805822520052 2212 16-17 0.7276880668660581 2212 18-19 0.6887485026623708 2212 20-21 0.7203251062504279 2212 22-23 0.7032627815057353 2212 24-25 0.774757198419401 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1.0871491373740731 2212 94-95 1.0707688220999678 2212 96-97 1.1402805084184493 2212 98-99 1.1673114581066848 2212 100 1.3818906992932618 2213 1 0.43554748007436217 2213 2 0.4124393056728195 2213 3 0.45481255839201395 2213 4 0.2791750294057209 2213 5 0.36885586507043655 2213 6 0.37016796652581263 2213 7 0.43144238098953736 2213 8 0.4258780807610236 2213 9 0.5518791976873914 2213 10-11 0.5170675987560571 2213 12-13 0.49860434067929305 2213 14-15 0.6825581883254586 2213 16-17 0.7061806691202293 2213 18-19 0.6964698630430775 2213 20-21 0.57660885633031 2213 22-23 0.6998598267232978 2213 24-25 0.7364836056289263 2213 26-27 0.7484930591313059 2213 28-29 0.7703787693082589 2213 30-31 0.7545802296498394 2213 32-33 0.8163374959278968 2213 34-35 0.8140626763747392 2213 36-37 0.8594098677864963 2213 38-39 0.7552916247374171 2213 40-41 0.7468569606654683 2213 42-43 0.8033420706361127 2213 44-45 0.8759525159114006 2213 46-47 0.7240804448621532 2213 48-49 1.9370559596924082 2213 50-51 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0.4001185111650045 2311 8 0.1820406199101825 2311 9 0.4834068095343369 2311 10-11 0.39348600611845796 2311 12-13 0.4909963842623952 2311 14-15 0.48832142042472526 2311 16-17 0.5046751265522218 2311 18-19 0.671351603612564 2311 20-21 0.689158322845941 2311 22-23 0.7084970458793833 2311 24-25 0.6274830582006388 2311 26-27 0.6551610685466187 2311 28-29 0.7442318439157418 2311 30-31 0.6653481498928571 2311 32-33 0.6679591690386104 2311 34-35 0.7837515833199689 2311 36-37 0.752576924079797 2311 38-39 0.8062944800866703 2311 40-41 0.7806713595214916 2311 42-43 0.8371263551978316 2311 44-45 0.8516273905595071 2311 46-47 0.8773308892889986 2311 48-49 -5.745098285401681 2311 50-51 -0.9500319365346357 2311 52-53 0.4488662892130648 2311 54-55 0.8303080019473157 2311 56-57 0.943064370741979 2311 58-59 0.9494765090212383 2311 60-61 0.770157261578774 2311 62-63 0.7019737301355775 2311 64-65 0.7778007228451642 2311 66-67 0.9315837634823936 2311 68-69 0.9271612847204338 2311 70-71 0.9529817299307481 2311 72-73 0.4748102146230977 2311 74-75 0.7895920031623902 2311 76-77 0.8190478047451926 2311 78-79 0.8003096121106523 2311 80-81 0.7878757246350006 2311 82-83 0.8201159926168167 2311 84-85 0.8486411058590164 2311 86-87 0.8893411732508696 2311 88-89 0.8660287686089312 2311 90-91 0.9853651537064643 2311 92-93 0.9005771298092604 2311 94-95 0.9387478631267676 2311 96-97 0.9509732030311824 2311 98-99 1.0405959204153383 2311 100 1.174953562708545 2312 1 0.10885536399067064 2312 2 0.224007978649567 2312 3 0.19316967790584982 2312 4 0.010141350837642449 2312 5 0.15766239722376696 2312 6 0.07069422682539539 2312 7 0.2690786751175125 2312 8 0.3261022774499125 2312 9 0.20650223294362746 2312 10-11 0.199189894390436 2312 12-13 0.2744912020453967 2312 14-15 0.29364471838768935 2312 16-17 0.4480443176040225 2312 18-19 0.3215924971657955 2312 20-21 0.31502224259516254 2312 22-23 0.5619521519746726 2312 24-25 0.7253261069146859 2312 26-27 0.5764157482766379 2312 28-29 0.7195947350937786 2312 30-31 0.7665215143343076 2312 32-33 0.7504249193774015 2312 34-35 0.3919341308261579 2312 36-37 0.7229666647580331 2312 38-39 0.8273591401658038 2312 40-41 0.6348185453049311 2312 42-43 0.6551951439146038 2312 44-45 0.7689123672218514 2312 46-47 0.9119455471811335 2312 48-49 -4.83466918894019 2312 50-51 -0.7801944279611774 2312 52-53 0.5584111260952156 2312 54-55 0.8415021336448021 2312 56-57 1.01392011879841 2312 58-59 1.03721615277329 2312 60-61 1.0180010230748167 2312 62-63 0.8324325028982003 2312 64-65 1.0574198919418265 2312 66-67 1.0397810227602235 2312 68-69 0.898358634154242 2312 70-71 0.9396943789015708 2312 72-73 1.009315023809414 2312 74-75 0.676180603739752 2312 76-77 0.8529186880095558 2312 78-79 0.5133097618678519 2312 80-81 0.7475628491016764 2312 82-83 0.8109647841156367 2312 84-85 0.8729247246838412 2312 86-87 0.9008484972196271 2312 88-89 0.9433836896193064 2312 90-91 0.781437830758474 2312 92-93 0.9444952408406486 2312 94-95 1.056397894856513 2312 96-97 1.1134035626494025 2312 98-99 1.1084521456221417 2312 100 0.7245421585664609 2313 1 0.10284505747744532 2313 2 0.2015100933388254 2313 3 0.2962259745001816 2313 4 0.09299719828720043 2313 5 0.17720998167683888 2313 6 0.2754960761052274 2313 7 0.18080441596753616 2313 8 0.2706472554949073 2313 9 0.48663246402633575 2313 10-11 0.27133518563594805 2313 12-13 0.08675107664344495 2313 14-15 0.14434775907175634 2313 16-17 0.13716254549195384 2313 18-19 0.4274443012805449 2313 20-21 0.45325221058766374 2313 22-23 0.5792720093977337 2313 24-25 0.34139857461821776 2313 26-27 0.4352309169315802 2313 28-29 0.45964617279378217 2313 30-31 0.6194244851838633 2313 32-33 0.5457387175902682 2313 34-35 0.41940902303103655 2313 36-37 -0.2586498198289249 2313 38-39 0.20788312359824346 2313 40-41 0.4010755855148105 2313 42-43 0.5373969200522808 2313 44-45 0.5437489771678514 2313 46-47 0.4396261795783687 2313 48-49 -5.247398919749251 2313 50-51 -1.302787312009663 2313 52-53 0.14001278769679004 2313 54-55 0.3131265374451573 2313 56-57 0.29512188525660576 2313 58-59 0.39012051587690877 2313 60-61 0.6879889087769513 2313 62-63 0.8167463682165135 2313 64-65 0.8054392900066247 2313 66-67 0.8714204952094917 2313 68-69 0.8567393146728293 2313 70-71 0.867893281788497 2313 72-73 0.6874310273093087 2313 74-75 0.8259082939142175 2313 76-77 0.7847773084796152 2313 78-79 0.7427478419835083 2313 80-81 -0.18463607201112353 2313 82-83 0.19145088738903482 2313 84-85 0.5801354688060201 2313 86-87 0.7251526170006315 2313 88-89 0.6947407847394409 2313 90-91 0.7609072426174421 2313 92-93 0.7853968190022051 2313 94-95 0.6944739916540676 2313 96-97 0.6998148954672274 2313 98-99 0.8853437466621088 2313 100 1.0164977013751582 2314 1 0.06406739870421063 2314 2 0.19444680017406313 2314 3 0.22618401178693404 2314 4 0.07285114696391304 2314 5 0.21691845611540828 2314 6 0.2952962578338685 2314 7 0.1522068996601149 2314 8 0.2717841779486676 2314 9 0.44873312605465543 2314 10-11 0.345718731375797 2314 12-13 0.27826147803077816 2314 14-15 0.2862879056557759 2314 16-17 0.5386797707491766 2314 18-19 0.5986852866806984 2314 20-21 0.6353094210647043 2314 22-23 0.3066807303263275 2314 24-25 0.49250482034983634 2314 26-27 0.5039343588802723 2314 28-29 0.6288575687316182 2314 30-31 0.5436262254076709 2314 32-33 0.5073555274883859 2314 34-35 0.37403987749586065 2314 36-37 0.4512590949353168 2314 38-39 0.5296771067250461 2314 40-41 0.322599821801532 2314 42-43 0.3818729153625853 2314 44-45 0.6092264993376801 2314 46-47 0.6839171723995818 2314 48-49 -6.16909591165021 2314 50-51 -1.395075831549427 2314 52-53 -0.7326080207470014 2314 54-55 -0.3022256773693286 2314 56-57 0.06118444944677748 2314 58-59 0.4801870148813734 2314 60-61 0.7410394597585821 2314 62-63 0.6199865787133945 2314 64-65 0.7945813441714549 2314 66-67 0.8754423867121375 2314 68-69 0.8806347142465469 2314 70-71 0.6615710632744012 2314 72-73 0.8463508520229439 2314 74-75 0.8730988701879383 2314 76-77 0.7730152972796525 2314 78-79 0.5781776925513995 2314 80-81 0.6430901890883831 2314 82-83 0.6763633595155163 2314 84-85 0.6951705237801349 2314 86-87 0.798855301698481 2314 88-89 0.6877338475449299 2314 90-91 0.8747257831675164 2314 92-93 0.8960204326670116 2314 94-95 0.9547155563473524 2314 96-97 1.0064755781124681 2314 98-99 1.0763443548326173 2314 100 -0.2347112844692738 2315 1 0.1413109156356711 2315 2 0.17010078721371258 2315 3 0.2652107902732226 2315 4 0.11729154962490185 2315 5 0.1857573851642158 2315 6 0.24713756878452386 2315 7 0.3076699752956884 2315 8 0.20034752798687094 2315 9 0.34571860789552744 2315 10-11 0.14796775676555285 2315 12-13 0.39389771384436756 2315 14-15 0.2172309780322692 2315 16-17 0.361823465687074 2315 18-19 0.39941756005401885 2315 20-21 0.3237829338434324 2315 22-23 0.38314065834988753 2315 24-25 0.4171796577067468 2315 26-27 0.4490181588828932 2315 28-29 0.5901756859044411 2315 30-31 0.5578373474497269 2315 32-33 0.5848371774559524 2315 34-35 0.6304191753385737 2315 36-37 0.5595005833713387 2315 38-39 0.6186774211915349 2315 40-41 0.6616194241362834 2315 42-43 0.6834408506859617 2315 44-45 0.6864652874497423 2315 46-47 0.4874136872428707 2315 48-49 -4.856313407412458 2315 50-51 -0.943154265137629 2315 52-53 0.17363346394630952 2315 54-55 0.15313797091619108 2315 56-57 0.5515368118220749 2315 58-59 0.6959258918492992 2315 60-61 0.5198665585786841 2315 62-63 0.27877805966231506 2315 64-65 0.47055011594454044 2315 66-67 -0.28693054181422895 2315 68-69 0.45929534181490084 2315 70-71 0.46198871327684543 2315 72-73 0.5496303656017787 2315 74-75 0.7347493583718645 2315 76-77 0.6359310808271594 2315 78-79 0.5765377895271584 2315 80-81 0.4647270154665222 2315 82-83 0.40341104001110395 2315 84-85 0.4518581417688097 2315 86-87 0.5140434336885278 2315 88-89 0.4995732156455652 2315 90-91 0.5735978073407928 2315 92-93 0.6350607409555593 2315 94-95 0.24728997191261826 2315 96-97 0.40569047418943427 2315 98-99 0.3741431017246093 2315 100 0.4660955272001033 2316 1 0.22243379777688688 2316 2 0.2309038601312281 2316 3 0.15858235878559412 2316 4 -0.07618788947553412 2316 5 0.1337735873839705 2316 6 0.1207012895500128 2316 7 0.25157914327341047 2316 8 0.2644757726792477 2316 9 0.46548736917149824 2316 10-11 0.4365248395499606 2316 12-13 0.27109166003045004 2316 14-15 0.43708277519112215 2316 16-17 0.4289693393834497 2316 18-19 0.1664429179633231 2316 20-21 0.49680165373838747 2316 22-23 0.5683901517852163 2316 24-25 0.5888486786200886 2316 26-27 0.634852912708368 2316 28-29 0.6568219107005078 2316 30-31 0.6532998353753996 2316 32-33 0.5767014083102424 2316 34-35 0.5417999132112641 2316 36-37 0.45338356453338946 2316 38-39 0.4926603682786208 2316 40-41 0.14429539061782037 2316 42-43 0.1544537193740254 2316 44-45 0.36680399207111947 2316 46-47 0.35337055299996223 2316 48-49 -5.0530638839745805 2316 50-51 -1.0033322627507957 2316 52-53 0.2820334650860943 2316 54-55 0.5459638112234799 2316 56-57 0.42017682693290226 2316 58-59 0.3200820216170044 2316 60-61 0.43109403508713484 2316 62-63 0.3609862003476252 2316 64-65 0.3601853912622417 2316 66-67 0.4612099237395313 2316 68-69 0.22368782626254813 2316 70-71 -0.24574453163212695 2316 72-73 -0.10836890136473443 2316 74-75 0.11562992058107824 2316 76-77 0.18675384311228882 2316 78-79 0.27776085246755144 2316 80-81 0.21477074146704211 2316 82-83 0.11984700358685885 2316 84-85 0.3361866023708835 2316 86-87 0.3550154207420171 2316 88-89 0.16173108443026507 2316 90-91 0.22324524466786855 2316 92-93 0.34814998436164757 2316 94-95 0.488781191570947 2316 96-97 0.5638073398436383 2316 98-99 0.5669040041396762 2316 100 -0.1313664987572487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 9.0 11 20.0 12 35.0 13 180.0 14 612.0 15 1826.0 16 4232.0 17 8870.0 18 15122.0 19 22848.0 20 32399.0 21 45388.0 22 57259.0 23 63259.0 24 67432.0 25 74605.0 26 88743.0 27 108554.0 28 133733.0 29 165072.0 30 203586.0 31 251559.0 32 315528.0 33 411277.0 34 566886.0 35 796699.0 36 1228186.0 37 1786887.0 38 1376587.0 39 168006.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.59538067194594 19.35181228030843 14.08509470258065 23.967712345164983 2 15.622376172213531 20.073126037414525 39.89527765093142 24.409220139440517 3 18.918236432491806 25.092756378755954 29.720976055377797 26.268031133374446 4 13.104551132910617 16.4739222018255 37.23653176055318 33.184994904710706 5 13.735935936987595 37.300137654529905 34.2470862540833 14.716840154399206 6 31.924145997729696 36.33683917726817 17.420928402438797 14.31808642256334 7 28.29631189783442 32.00123620881123 21.437154028190914 18.265297865163436 8 26.520142552509423 35.30034499928644 19.496230748650827 18.683281699553316 9 26.553719664698882 16.010121825906346 20.10024260110412 37.33591590829065 10-11 25.54966516670623 25.97872230786953 27.462133482553924 21.009479042870318 12-13 25.785380446790178 23.493139843949187 27.905322160837688 22.816157548422947 14-15 23.1799639918178 25.034860648084745 25.448567020682507 26.33660833941495 16-17 22.705455304983847 27.92398612574195 26.2599381580556 23.110620411218598 18-19 22.461831090808364 27.08111630735342 28.341857698559593 22.115194903278624 20-21 23.312737761475084 26.282917644728077 28.21178731591934 22.1925572778775 22-23 23.286772940870335 25.974666765640407 28.065034754730117 22.673525538759133 24-25 22.90921200125697 26.14830691974929 28.275851933843317 22.66662914515042 26-27 22.871025109534735 26.32221190935948 28.0566822785426 22.75008070256318 28-29 22.684524179903825 26.440818090315876 28.196112041689346 22.67854568809095 30-31 23.07165116047826 26.28749517679056 28.126430937581887 22.514422725149295 32-33 22.99017224495075 26.487644226986628 27.880627718474578 22.64155580958804 34-35 23.12407691154407 26.40529769976036 28.073579246735424 22.397046141960146 36-37 23.057374426201946 26.28321598625712 27.963577662614377 22.69583192492656 38-39 23.0010187377822 26.383807379684704 28.056064792398477 22.55910909013462 40-41 23.019937738890327 26.09526357532465 28.233583350875463 22.651215334909562 42-43 22.83291486356101 26.216241096625115 27.97168781196052 22.979156227853352 44-45 22.76540057476875 26.513474218867806 27.906035422120933 22.81508978424251 46-47 22.81497455436522 26.36803200202139 27.642246320749464 23.17474712286392 48-49 23.089749427870835 26.49115970711747 27.51443441659663 22.90465644841507 50-51 22.89338269557097 26.544405686039408 27.44684271197084 23.11536890641878 52-53 22.841296728730835 26.697509648256908 27.285943548924802 23.175250074087455 54-55 22.776893679869136 26.603138785581987 27.395931217773377 23.224036316775496 56-57 22.964776595981537 26.557925541083087 27.17782863306865 23.29946922986672 58-59 22.702267923401852 26.57938757581316 27.457978133488204 23.260366367296783 60-61 22.875944074539596 26.701272883211118 27.29844619117526 23.12433685107403 62-63 22.833898481470438 26.641720471908858 27.27335552148761 23.251025525133098 64-65 22.633972446901925 26.470067434455263 27.316805711047177 23.57915440759563 66-67 22.732478318320904 26.42267370302136 27.203410568594073 23.641437410063663 68-69 22.51080758996233 26.507199732469573 27.266666370578307 23.71532630698979 70-71 22.59150959040061 26.258379821260924 27.357482597951165 23.7926279903873 72-73 22.672494574257566 26.50784724016625 27.092157963992847 23.727500221583337 74-75 22.63541646666386 26.63669006368548 27.02373497274031 23.704158496910352 76-77 22.549125381544595 26.52867860027273 27.02636354874455 23.895832469438126 78-79 22.444635555386835 26.47711952350876 27.09072657396403 23.987518347140373 80-81 22.422108225989707 26.692747745020885 26.955893263904972 23.929250765084433 82-83 22.633111123133553 26.46284872079289 27.222101476581926 23.681938679491626 84-85 22.638400082566697 26.538594458335872 26.87528519131861 23.947720267778816 86-87 22.299902684319015 26.717845699288667 27.0662443440499 23.91600727234242 88-89 22.36364953445424 26.33569239211363 27.09966636544013 24.200991707992003 90-91 22.46726882267088 26.27833262924873 27.112006746202166 24.142391801878222 92-93 22.436877852081356 26.39951110661351 27.018108093672293 24.145502947632842 94-95 22.106252342120445 26.230673284892404 27.197359263889993 24.465715109097157 96-97 22.10014535154599 26.222608375456012 27.194610098011296 24.482636174986702 98-99 21.975843386496745 26.29136232599801 27.053034131125415 24.67976015637983 100 21.949617421305998 26.19570542966973 27.06074265658601 24.79393449243826 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1798.0 1 1294.5 2 1510.5 3 2099.5 4 1534.0 5 1218.0 6 1796.0 7 3337.0 8 5297.0 9 6240.5 10 5679.0 11 4907.5 12 4963.0 13 5514.5 14 6234.5 15 7017.0 16 7620.5 17 8282.0 18 9108.5 19 9886.0 20 10822.5 21 11882.5 22 13217.5 23 15366.5 24 18035.5 25 21528.5 26 26662.0 27 32984.0 28 40265.5 29 49999.0 30 63580.0 31 77770.5 32 95660.0 33 116804.0 34 136276.5 35 154915.0 36 177009.0 37 201575.0 38 216798.5 39 223978.5 40 228586.5 41 233085.0 42 241903.5 43 255957.0 44 270342.5 45 288357.0 46 305046.0 47 316275.0 48 328549.5 49 334496.5 50 332118.5 51 329204.5 52 324683.5 53 316684.5 54 305108.5 55 284283.0 56 258346.5 57 228661.0 58 199769.0 59 175051.5 60 146169.0 61 117466.5 62 94070.5 63 73487.5 64 54798.0 65 39617.5 66 29292.5 67 22228.0 68 16653.0 69 12312.5 70 9353.5 71 7108.0 72 5240.0 73 3759.5 74 2751.5 75 1901.0 76 1262.0 77 753.5 78 437.0 79 252.5 80 149.5 81 94.5 82 56.0 83 31.5 84 22.0 85 18.5 86 14.5 87 10.0 88 7.5 89 8.5 90 6.0 91 2.5 92 1.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029904639096282055 2 0.0022262759343949 3 1.0005734536606293E-4 4 0.02197509447602157 5 4.62765222318041E-4 6 3.75215045122736E-5 7 2.5014336341515733E-5 8 0.004077336823667064 9 0.010130806218313871 10-11 0.0011631666398804815 12-13 8.129659310992612E-5 14-15 1.2507168170757866E-4 16-17 4.0648296554963065E-4 18-19 6.253584085378933E-6 20-21 8.755017719530506E-5 22-23 3.5645429286659916E-4 24-25 3.1267920426894665E-5 26-27 7.50430090245472E-5 28-29 0.0010943772149413133 30-31 0.0027515769975667305 32-33 0.010155820554655387 34-35 0.019142220885344913 36-37 0.021875037130655507 38-39 0.02125593230620299 40-41 0.004702695232204957 42-43 0.005165460454522998 44-45 0.0090239218352018 46-47 0.011506594717097236 48-49 1.2058410976791074 50-51 0.018135393847598904 52-53 0.018560637565404672 54-55 0.009536715730202873 56-57 0.007716922761357604 58-59 0.008486113603859213 60-61 0.005202981959035272 62-63 0.007610611831906161 64-65 0.018817034512905208 66-67 0.028278707234083537 68-69 0.02883527621768226 70-71 0.025408312138894603 72-73 0.0226192136368156 74-75 0.00919902218959241 76-77 0.013764138571919032 78-79 0.008010841213370413 80-81 0.0027390698293959723 82-83 0.013851688749114336 84-85 0.02348220824059789 86-87 0.02303820377053599 88-89 0.03174319281738346 90-91 0.019811354382480457 92-93 0.012932411888563634 94-95 0.006541248953306364 96-97 0.003952265141959485 98-99 0.016127993356192267 100 0.03515764972800036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7995415.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.526032028323556 #Duplication Level Percentage of deduplicated Percentage of total 1 73.26498163278346 42.14643679962038 2 15.241676513499364 17.535863425618228 3 5.228290481597254 9.022884170932285 4 2.3109920695396373 5.317688152381558 5 1.1981981808775868 3.4463793464721544 6 0.7054462834762311 2.4348915298509293 7 0.45380207275108486 1.8273802800118966 8 0.30907220121122575 1.422375787675315 9 0.22954280611781966 1.1884218134944464 >10 0.9868931317822213 9.706892466724867 >50 0.046196919079312775 1.8065926783355508 >100 0.023257735318102744 2.3581603374560953 >500 0.0010901693464206576 0.4094640525210697 >1k 4.7141273292752856E-4 0.48331953690238777 >5k 0.0 0.0 >10k+ 8.838988735106063E-5 0.8932496220027423 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22683 0.2837000956173007 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18591 0.23252076346255948 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11428 0.1429319178554209 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.002867268303146E-5 0.0 0.0 0.0 8.755017719530506E-5 7 5.002867268303146E-5 0.0 0.0 0.0 8.755017719530506E-5 8 5.002867268303146E-5 0.0 0.0 0.0 1.0005734536606292E-4 9 5.002867268303146E-5 0.0 0.0 0.0 1.2507168170757866E-4 10-11 5.002867268303146E-5 0.0 0.0 0.0 1.2507168170757866E-4 12-13 5.002867268303146E-5 0.0 0.0 0.0 1.3757884987833653E-4 14-15 5.002867268303146E-5 0.0 0.0 0.0 1.5633960213447333E-4 16-17 1.3757884987833653E-4 0.0 0.0 1.2507168170757865E-5 1.87607522561368E-4 18-19 1.3757884987833653E-4 0.0 0.0 1.8760752256136797E-5 1.87607522561368E-4 20-21 1.3757884987833653E-4 0.0 0.0 8.129659310992612E-5 2.0011469073212584E-4 22-23 1.3757884987833653E-4 0.0 0.0 3.814686292081149E-4 2.3763619524439945E-4 24-25 1.3757884987833653E-4 0.0 0.0 9.818127014044925E-4 2.7515769975667307E-4 26-27 1.3757884987833653E-4 0.0 0.0 0.002213768766224142 2.7515769975667307E-4 28-29 1.3757884987833653E-4 0.0 0.0 0.005734536606292482 2.7515769975667307E-4 30-31 1.5008601804909438E-4 1.2507168170757865E-5 0.0 0.01811663309534277 3.001720360981888E-4 32-33 1.5008601804909438E-4 1.2507168170757865E-5 0.0 0.045269695194058095 3.376935406104624E-4 34-35 1.5008601804909438E-4 1.2507168170757865E-5 0.0 0.08836314312640432 3.5020070878122024E-4 36-37 1.5633960213447333E-4 1.2507168170757865E-5 0.0 0.15675859226819372 3.5020070878122024E-4 38-39 1.6259318621985225E-4 2.501433634151573E-5 0.0 0.25131903722320853 3.5645429286659916E-4 40-41 1.7510035439061012E-4 3.7521504512273594E-5 0.0 0.3540216486573868 3.75215045122736E-4 42-43 1.8135393847598907E-4 3.7521504512273594E-5 0.0 0.45848777080364184 3.814686292081149E-4 44-45 1.9386110664674691E-4 3.7521504512273594E-5 0.0 0.5845725331330518 4.2524371780576747E-4 46-47 2.0011469073212584E-4 3.7521504512273594E-5 0.0 0.731369165953237 4.62765222318041E-4 48-49 2.0011469073212584E-4 3.7521504512273594E-5 0.0 0.8912920717686323 4.8777955865955675E-4 50-51 2.0636827481750476E-4 3.7521504512273594E-5 0.0 1.0627340794692959 4.940331427449357E-4 52-53 2.126218589028837E-4 3.7521504512273594E-5 0.0 1.22824768945702 5.56568983598725E-4 54-55 2.126218589028837E-4 3.7521504512273594E-5 0.0 1.4143480982538117 6.128512403671355E-4 56-57 2.126218589028837E-4 3.7521504512273594E-5 0.0 1.626994971493037 6.191048244525144E-4 58-59 2.126218589028837E-4 3.7521504512273594E-5 0.0 1.8664196917858549 6.316119926232722E-4 60-61 2.75157699756673E-4 3.7521504512273594E-5 0.0 2.1091275437234964 6.628799130501668E-4 62-63 2.876648679274309E-4 3.7521504512273594E-5 0.0 2.356019043414257 6.628799130501668E-4 64-65 2.9391845201280983E-4 3.7521504512273594E-5 0.0 2.607613238337222 6.628799130501668E-4 66-67 3.1267920426894665E-4 3.7521504512273594E-5 0.0 2.892951772984892 6.816406653063036E-4 68-69 3.1267920426894665E-4 3.7521504512273594E-5 0.0 3.2071380910184146 7.004014175624405E-4 70-71 3.314399565250834E-4 3.7521504512273594E-5 0.0 3.5409043808232594 7.50430090245472E-4 72-73 4.0022938146425167E-4 3.7521504512273594E-5 0.0 3.8678042353023576 9.880662854898714E-4 74-75 4.0022938146425167E-4 3.7521504512273594E-5 0.0 4.195016768985725 9.880662854898714E-4 76-77 4.189901337203885E-4 3.7521504512273594E-5 0.0 4.554041284911415 9.880662854898714E-4 78-79 4.252437178057674E-4 3.7521504512273594E-5 0.0 4.9424013637816175 9.880662854898714E-4 80-81 4.252437178057674E-4 3.7521504512273594E-5 0.0 5.349190754951431 0.0010068270377460082 82-83 4.377508859765253E-4 3.7521504512273594E-5 0.0 5.7624400984814415 0.0011006307990266923 84-85 4.4400447006190424E-4 3.7521504512273594E-5 0.0 6.172531382048336 0.0011068843831120712 86-87 4.5025805414728316E-4 5.002867268303146E-5 0.0 6.6004516338426455 0.0011318987194535869 88 4.5025805414728316E-4 5.002867268303146E-5 0.0 6.939001915472805 0.0011756738080512393 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12715 0.0 49.347767 1 GTATCAA 22940 0.0 41.386147 1 CAACGCA 28630 0.0 32.641163 5 ATCAACG 28765 0.0 32.446957 3 TATCAAC 29340 0.0 32.11133 2 TCAACGC 29770 0.0 31.430595 4 AACGCAG 30850 0.0 30.581202 6 ACGCAGA 34810 0.0 26.765347 7 CGCAGAG 36015 0.0 25.843771 8 TACCTGG 6365 0.0 25.800274 2 GTACATG 31025 0.0 25.609297 1 GTGGTAT 6065 0.0 24.374304 1 TACATGG 32105 0.0 24.245344 2 ACATGGG 32650 0.0 23.182207 3 GCAGAGT 39960 0.0 22.985842 9 GTACCTG 7730 0.0 22.1598 1 CATGGGG 22205 0.0 21.576513 4 TGGTATC 6725 0.0 21.27954 2 GAGTACT 21905 0.0 20.73749 12-13 ATGGGGG 14395 0.0 20.108103 5 >>END_MODULE