FastQCFastQC Report
Mon 6 Jun 2016
ERR1211320_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211320_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9909095
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353180.35642003634035196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275870.27840080249508153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163210.16470727145112646No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160650.051.910581
GTATCAA297800.042.2658161
CAACGCA362250.033.780295
ATCAACG364200.033.612493
TATCAAC381600.032.5521052
TCAACGC382850.032.0729834
AACGCAG389600.031.8071046
GTACATG417000.028.1086391
ACGCAGA440550.027.7769997
TACATGG430750.026.9269312
CGCAGAG454300.026.9034928
GTGGTAT74200.026.3704341
ACATGGG432950.025.9847983
GTACTAG37650.025.1107031
TACCTGG69000.024.6762142
GCAGAGT491000.024.2339349
CATGGGG296050.023.7784
TGGTATC79200.023.3986112
ATGGGGG204350.021.7774035
GAGTACT272100.021.68259612-13
Produced by FastQC (version 0.11.5)