FastQCFastQC Report
Mon 6 Jun 2016
ERR1211320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9909095
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307950.3107751010561509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258030.2603971402030155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159160.160620117175181No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165800.051.996881
GTATCAA296550.041.161871
CAACGCA372100.032.0610055
ATCAACG372900.031.973033
TATCAAC388600.031.065462
TCAACGC392500.030.496734
AACGCAG396900.030.3648576
GTACATG412850.027.0316281
GTACTAG37150.026.9075031
ACGCAGA449550.026.516427
TACATGG429300.025.8798452
GTGGTAT76400.025.7379131
CGCAGAG466600.025.4970868
ACATGGG427700.024.9143413
TACCTGG66100.024.4882892
CATGGGG289100.023.1285954
GCAGAGT509150.023.1254339
TGGTATC81800.023.0575712
ATGGGGG190050.021.4507435
GAGTACT281250.021.43844412-13
Produced by FastQC (version 0.11.5)