##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211320_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9909095 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.094303970241484 33.0 31.0 34.0 30.0 34.0 2 32.22326761424731 33.0 31.0 34.0 30.0 34.0 3 32.28810481683746 34.0 31.0 34.0 30.0 34.0 4 35.93250705538699 37.0 35.0 37.0 35.0 37.0 5 35.83696654437161 37.0 35.0 37.0 35.0 37.0 6 35.85326682204581 37.0 35.0 37.0 35.0 37.0 7 35.76682643571386 37.0 35.0 37.0 33.0 37.0 8 35.74842011303757 37.0 35.0 37.0 33.0 37.0 9 37.35971498910849 39.0 37.0 39.0 34.0 39.0 10-11 37.30987315188723 39.0 37.0 39.0 34.0 39.0 12-13 37.24089737761118 39.0 37.0 39.0 33.0 39.0 14-15 38.46622804605264 40.0 38.0 41.0 34.0 41.0 16-17 38.449545745600375 40.0 38.0 41.0 34.0 41.0 18-19 38.456622930752005 40.0 38.0 41.0 34.0 41.0 20-21 38.42686743844922 40.0 38.0 41.0 34.0 41.0 22-23 38.32061691809393 40.0 38.0 41.0 34.0 41.0 24-25 38.23163290895889 40.0 38.0 41.0 33.5 41.0 26-27 38.13126582195448 40.0 38.0 41.0 33.0 41.0 28-29 38.02947423553816 40.0 37.0 41.0 33.0 41.0 30-31 38.03557080641572 40.0 37.0 41.0 33.0 41.0 32-33 37.972392080205104 40.0 37.0 41.0 33.0 41.0 34-35 37.84688980174275 40.0 37.0 41.0 33.0 41.0 36-37 37.70251481088838 40.0 37.0 41.0 33.0 41.0 38-39 37.588694578061876 40.0 37.0 41.0 32.5 41.0 40-41 37.47695475722051 40.0 36.0 41.0 32.0 41.0 42-43 37.37057607178052 40.0 36.0 41.0 32.0 41.0 44-45 37.21275595803653 39.5 36.0 41.0 31.0 41.0 46-47 36.96863467349945 39.0 36.0 41.0 31.0 41.0 48-49 35.39970118360961 39.0 35.0 40.5 24.0 41.0 50-51 36.13519766436794 39.0 35.0 40.0 29.0 41.0 52-53 36.23191149141269 39.0 35.0 40.0 29.5 41.0 54-55 36.06052237868342 38.0 35.0 40.0 29.0 41.0 56-57 35.8462993845553 38.0 35.0 40.0 28.5 41.0 58-59 35.60533075926712 38.0 34.0 40.0 28.0 41.0 60-61 35.34797118203025 37.5 34.0 40.0 28.0 41.0 62-63 35.025551223396285 37.0 34.0 40.0 27.5 41.0 64-65 34.68799562422199 36.5 33.5 39.0 26.5 41.0 66-67 34.35046157091036 36.0 33.0 39.0 26.0 40.5 68-69 33.99299446619494 35.5 33.0 39.0 26.0 40.0 70-71 33.63807572739993 35.0 32.5 38.0 26.0 40.0 72-73 33.26459843204652 35.0 32.0 37.0 26.0 39.5 74-75 32.904318911061 35.0 32.0 37.0 25.0 39.0 76-77 31.969966984876017 34.0 30.5 36.0 24.5 38.0 78-79 32.41231838023553 34.5 31.5 36.0 25.0 38.0 80-81 32.29044221495505 35.0 32.0 35.5 25.0 37.0 82-83 32.02433214133077 34.0 31.0 35.0 25.0 37.0 84-85 31.742904927241085 34.0 31.0 35.0 24.0 36.5 86-87 31.484530827487276 34.0 31.0 35.0 24.0 36.0 88-89 31.25740039832094 34.0 31.0 35.0 23.0 36.0 90-91 31.04197093680099 34.0 30.5 35.0 23.0 35.0 92-93 30.847179030981135 34.0 30.0 35.0 20.5 35.0 94-95 30.649179970522027 34.0 30.0 35.0 20.0 35.0 96-97 30.453974908909444 34.0 29.5 35.0 20.0 35.0 98-99 30.232344376555073 34.0 29.0 35.0 19.0 35.0 100 27.795062818552047 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2287671610386255 1101 2 -0.22402538419191487 1101 3 -0.2669685431524407 1101 4 -0.19555182502551816 1101 5 -0.1858990569929091 1101 6 -0.22209483938065944 1101 7 -0.2148513296933885 1101 8 -0.2246453400066386 1101 9 -0.2786197019313832 1101 10-11 -0.31173548228625947 1101 12-13 -0.43923057065292426 1101 14-15 -0.5110502398382408 1101 16-17 -0.43439867260639176 1101 18-19 -0.47717893335710215 1101 20-21 -0.5408564909560098 1101 22-23 -0.6279480558178108 1101 24-25 -0.5332606835323759 1101 26-27 -0.6112626992768426 1101 28-29 -0.5514924622683495 1101 30-31 -0.5381550492511096 1101 32-33 -0.515302799102237 1101 34-35 -0.573857958356534 1101 36-37 -0.6046772961999167 1101 38-39 -0.5179984679086047 1101 40-41 -0.5927124878529924 1101 42-43 -0.6075379854614411 1101 44-45 -0.5559062967403108 1101 46-47 -0.6002874407202086 1101 48-49 0.6267439691917076 1101 50-51 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1105 68-69 -0.8817620076309183 1105 70-71 -0.8328928406158411 1105 72-73 -0.7837475677419192 1105 74-75 -0.872944242357665 1105 76-77 -0.6991215360662579 1105 78-79 -0.6258725931060845 1105 80-81 -0.6692143412611316 1105 82-83 -0.6337157558503534 1105 84-85 -0.6578288635070813 1105 86-87 -0.6221835297082237 1105 88-89 -0.6978758408313688 1105 90-91 -0.69979582360682 1105 92-93 -0.7534317465804605 1105 94-95 -0.6526747789108356 1105 96-97 -0.7251613294031856 1105 98-99 -0.7275201006224314 1105 100 -0.7242829983541377 1106 1 -0.33894557485024635 1106 2 -0.3131452837928208 1106 3 -0.3432165177357014 1106 4 -0.2663509515782252 1106 5 -0.32721367265222767 1106 6 -0.37825250845699543 1106 7 -0.3657950941888899 1106 8 -0.35483566478657025 1106 9 -0.46071346829548077 1106 10-11 -0.4939863795512949 1106 12-13 -0.43244169103506636 1106 14-15 -0.5514719306094307 1106 16-17 -0.5757304429878118 1106 18-19 -0.6272259887598466 1106 20-21 -0.6517532555792371 1106 22-23 -0.6814427884857963 1106 24-25 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0.3167936029560323 2311 2 0.3650932025950411 2311 3 0.37058150880442753 2311 4 0.2527934164207579 2311 5 0.32135290008369566 2311 6 0.33293293049174366 2311 7 0.37673436704037755 2311 8 0.20029937653804808 2311 9 0.47055365659483783 2311 10-11 0.38207917364122324 2311 12-13 0.46661281247880737 2311 14-15 0.40133663682817655 2311 16-17 0.42368969428419234 2311 18-19 0.6270742813501613 2311 20-21 0.678618265737235 2311 22-23 0.6966523353645186 2311 24-25 0.6041310212138455 2311 26-27 0.6824080375890915 2311 28-29 0.7245421059201078 2311 30-31 0.5554682783736737 2311 32-33 0.5952984560431887 2311 34-35 0.7055715395110838 2311 36-37 0.7595192172620102 2311 38-39 0.7724395696629145 2311 40-41 0.74404594886051 2311 42-43 0.7818647288600076 2311 44-45 0.7752370702201574 2311 46-47 0.8338505610743354 2311 48-49 -6.071035321202235 2311 50-51 -1.138363505544305 2311 52-53 0.25689171568404845 2311 54-55 0.725169077588184 2311 56-57 0.8173778090534327 2311 58-59 0.824155545232685 2311 60-61 0.7219329434429227 2311 62-63 0.6173001055123848 2311 64-65 0.721968761000582 2311 66-67 0.7960029138495202 2311 68-69 0.798418262611186 2311 70-71 0.8306718226602854 2311 72-73 0.29293814392035955 2311 74-75 0.6196656100479387 2311 76-77 0.7365402942652821 2311 78-79 0.6617605040626486 2311 80-81 0.7205689914578386 2311 82-83 0.7446960897305281 2311 84-85 0.7509005186792699 2311 86-87 0.7917580510212225 2311 88-89 0.8019245168631528 2311 90-91 0.8476390749481872 2311 92-93 0.825596156087002 2311 94-95 0.8625434335306998 2311 96-97 0.9501900832000416 2311 98-99 0.9981926525051996 2311 100 1.1821494787606923 2312 1 0.053815378175883666 2312 2 0.24720098517269662 2312 3 0.24079104696584608 2312 4 0.0034164207253724044 2312 5 0.21357671967652436 2312 6 0.16080450396806611 2312 7 0.28615547034429767 2312 8 0.3393781591152134 2312 9 0.30107427627595484 2312 10-11 0.2680835583819672 2312 12-13 0.308590281783097 2312 14-15 0.39531258366415756 2312 16-17 0.49837460243601583 2312 18-19 0.3490770631296627 2312 20-21 0.41649609450688274 2312 22-23 0.61751888717734 2312 24-25 0.7437028423214542 2312 26-27 0.6993139654787441 2312 28-29 0.8258224995509309 2312 30-31 0.8368529593284606 2312 32-33 0.8639726921361941 2312 34-35 0.5200968231332439 2312 36-37 0.7784270145340955 2312 38-39 0.899638013962786 2312 40-41 0.6987739640017949 2312 42-43 0.7228303211747544 2312 44-45 0.8661930389475074 2312 46-47 0.9014327559487967 2312 48-49 -4.622500551452703 2312 50-51 -0.7177701609189597 2312 52-53 0.6128530172999191 2312 54-55 0.9310664190300173 2312 56-57 1.0371167267700585 2312 58-59 1.0248082932815095 2312 60-61 1.0725553980922058 2312 62-63 0.8874567635968305 2312 64-65 1.0765828604575916 2312 66-67 1.0355052551349218 2312 68-69 1.019289154699642 2312 70-71 1.0495540555702831 2312 72-73 1.1551515132872652 2312 74-75 0.7797082164969211 2312 76-77 0.9821394487629576 2312 78-79 0.6582286875173367 2312 80-81 0.8810133736353833 2312 82-83 0.851487340116666 2312 84-85 0.9282866179295901 2312 86-87 0.9710867700415982 2312 88-89 1.0434236389850469 2312 90-91 0.8813825240419142 2312 92-93 1.0419663216758295 2312 94-95 1.1500577222600619 2312 96-97 1.259473995148518 2312 98-99 1.2760216638744772 2312 100 0.9086179176434506 2313 1 0.06709722575170929 2313 2 0.21297218829263187 2313 3 0.2794655444087155 2313 4 0.055676554165451364 2313 5 0.143906540263103 2313 6 0.261522810444049 2313 7 0.15609360772838698 2313 8 0.23032282264485815 2313 9 0.4457627918912834 2313 10-11 0.21585507725690434 2313 12-13 0.026904476925352583 2313 14-15 0.10044454226332533 2313 16-17 0.06438923317075052 2313 18-19 0.3942038733137281 2313 20-21 0.3829385631747826 2313 22-23 0.4482767527964455 2313 24-25 0.2420209883172575 2313 26-27 0.4141122157466057 2313 28-29 0.41089032903865075 2313 30-31 0.561160369322927 2313 32-33 0.43885809689904676 2313 34-35 0.4142257750712446 2313 36-37 -0.3060122871852613 2313 38-39 0.13737251220573654 2313 40-41 0.3610511582128737 2313 42-43 0.5045584044280389 2313 44-45 0.42338758604964966 2313 46-47 0.2548184571574481 2313 48-49 -5.445164446431903 2313 50-51 -1.4146860769771763 2313 52-53 0.048311416623896264 2313 54-55 0.20048665435274415 2313 56-57 0.20584374852986542 2313 58-59 0.2732206732572564 2313 60-61 0.6129058039045532 2313 62-63 0.691532838471197 2313 64-65 0.767148744506521 2313 66-67 0.787157768806189 2313 68-69 0.7891558661459612 2313 70-71 0.814040325318679 2313 72-73 0.578218886041796 2313 74-75 0.7467240491912364 2313 76-77 0.72832005435707 2313 78-79 0.6997017865589754 2313 80-81 -0.21948115632042686 2313 82-83 0.14698576941478692 2313 84-85 0.5889962845209915 2313 86-87 0.7293682296261643 2313 88-89 0.692643379673143 2313 90-91 0.728268068788875 2313 92-93 0.8145605732780403 2313 94-95 0.7172210240162897 2313 96-97 0.7718048762947447 2313 98-99 0.9535801634978114 2313 100 0.9423601740849499 2314 1 0.04928339599106124 2314 2 0.14754325775712118 2314 3 0.18212161221546097 2314 4 0.046669355914339405 2314 5 0.17050966708469417 2314 6 0.2504598537985032 2314 7 0.07457456384818784 2314 8 0.2198057960946116 2314 9 0.39274476877322684 2314 10-11 0.2911922641547804 2314 12-13 0.20862283798909687 2314 14-15 0.22369693393551415 2314 16-17 0.44132090683022795 2314 18-19 0.5120353272755622 2314 20-21 0.5663516364204 2314 22-23 0.26635879566229903 2314 24-25 0.44154751447855034 2314 26-27 0.4452963889386794 2314 28-29 0.5787504033123767 2314 30-31 0.46648322578217716 2314 32-33 0.49125043189997797 2314 34-35 0.3611907072571938 2314 36-37 0.40911699208921704 2314 38-39 0.45977395847680924 2314 40-41 0.27201994526725315 2314 42-43 0.27752394103787736 2314 44-45 0.485816552297166 2314 46-47 0.5984275147074598 2314 48-49 -6.425829053392299 2314 50-51 -1.5398169201912566 2314 52-53 -0.7884366980465529 2314 54-55 -0.35954969800697256 2314 56-57 -0.10343147810917941 2314 58-59 0.3566184403224 2314 60-61 0.5786337047825043 2314 62-63 0.4579097199524682 2314 64-65 0.5803353992846425 2314 66-67 0.6428427218311441 2314 68-69 0.6618154312664473 2314 70-71 0.5124313762810075 2314 72-73 0.6752605773102687 2314 74-75 0.6827712849479255 2314 76-77 0.5945020120284141 2314 78-79 0.4072627825574173 2314 80-81 0.4591206150385574 2314 82-83 0.5263086987468064 2314 84-85 0.5476529944194688 2314 86-87 0.6059489404631471 2314 88-89 0.46338515933788216 2314 90-91 0.6279332958922446 2314 92-93 0.7390008998552844 2314 94-95 0.762754107568405 2314 96-97 0.7974974230106469 2314 98-99 0.922219741050128 2314 100 -0.5569986831192502 2315 1 0.08599808797621478 2315 2 0.18360079515791483 2315 3 0.29856173359922167 2315 4 0.12139159015382717 2315 5 0.20960425218859768 2315 6 0.27313543204266466 2315 7 0.3097827636014827 2315 8 0.2430996360129427 2315 9 0.4522719067479244 2315 10-11 0.22072315367029205 2315 12-13 0.37350685554277163 2315 14-15 0.22803991817753655 2315 16-17 0.4131418634150279 2315 18-19 0.46393366503949807 2315 20-21 0.3622615421884916 2315 22-23 0.4458906322104852 2315 24-25 0.45392427378008193 2315 26-27 0.49157264568871284 2315 28-29 0.5936545775956645 2315 30-31 0.5478795436760748 2315 32-33 0.6512394527779506 2315 34-35 0.6118077959022443 2315 36-37 0.5052730725448171 2315 38-39 0.6210367060209236 2315 40-41 0.6273161071375242 2315 42-43 0.6552224729865728 2315 44-45 0.6154372484910198 2315 46-47 0.5211454188552693 2315 48-49 -4.8901168385433 2315 50-51 -0.9464622269663465 2315 52-53 0.252956303080623 2315 54-55 0.1842230563364069 2315 56-57 0.48768279408221105 2315 58-59 0.6336201714550924 2315 60-61 0.5442905870949772 2315 62-63 0.3278644517904752 2315 64-65 0.5069275603077443 2315 66-67 -0.2010378543349276 2315 68-69 0.47410269597994414 2315 70-71 0.443315457113691 2315 72-73 0.6125708882792154 2315 74-75 0.7424503355734942 2315 76-77 0.6389570496073134 2315 78-79 0.5583170021153236 2315 80-81 0.48637380487060966 2315 82-83 0.4788166457499976 2315 84-85 0.5497724535518849 2315 86-87 0.5744910116365283 2315 88-89 0.5405735850488753 2315 90-91 0.6236397080137444 2315 92-93 0.6750234185704826 2315 94-95 0.3384506877688338 2315 96-97 0.4408039036628786 2315 98-99 0.33288989365798827 2315 100 0.462294438776123 2316 1 0.23347002255673743 2316 2 0.24108999606006165 2316 3 0.17898951217483727 2316 4 0.0017280067573253177 2316 5 0.16193409922617974 2316 6 0.15255334076842786 2316 7 0.2556246231126522 2316 8 0.27813914908389137 2316 9 0.4413752603498651 2316 10-11 0.4350474328503324 2316 12-13 0.3141000461327721 2316 14-15 0.424464044776542 2316 16-17 0.39877287287876584 2316 18-19 0.12856268331415066 2316 20-21 0.44147525981617974 2316 22-23 0.562916764229513 2316 24-25 0.5660528439919119 2316 26-27 0.5434575715605092 2316 28-29 0.6426051438656728 2316 30-31 0.5516546152724757 2316 32-33 0.5326676449483472 2316 34-35 0.5231800162806195 2316 36-37 0.4137062124679076 2316 38-39 0.5616071506804516 2316 40-41 0.16262313766458902 2316 42-43 0.07175532455134004 2316 44-45 0.32421707072811756 2316 46-47 0.3860256738244203 2316 48-49 -5.0609032245707155 2316 50-51 -1.0754905834457205 2316 52-53 0.27030245732098024 2316 54-55 0.5241179686827735 2316 56-57 0.4162808560401956 2316 58-59 0.3350250785429836 2316 60-61 0.4234800799793703 2316 62-63 0.3432146233534894 2316 64-65 0.3873409144573472 2316 66-67 0.4880911933668699 2316 68-69 0.18429285319535182 2316 70-71 -0.25467670933914377 2316 72-73 -0.15368276117148838 2316 74-75 0.13886187298737696 2316 76-77 0.19776424515694657 2316 78-79 0.29665991823569726 2316 80-81 0.21513042785890946 2316 82-83 0.1080206630784808 2316 84-85 0.26178683511924206 2316 86-87 0.2747488277023429 2316 88-89 0.10886594271956085 2316 90-91 0.18819047286802615 2316 92-93 0.2835629035381686 2316 94-95 0.5372762339815864 2316 96-97 0.5841856333095521 2316 98-99 0.5693434863842519 2316 100 -0.15156703745978817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 4.0 10 9.0 11 17.0 12 61.0 13 189.0 14 778.0 15 2257.0 16 5454.0 17 11491.0 18 19379.0 19 29147.0 20 41716.0 21 59027.0 22 74518.0 23 79598.0 24 82717.0 25 90927.0 26 107913.0 27 132370.0 28 163294.0 29 200938.0 30 247401.0 31 306585.0 32 386799.0 33 501864.0 34 695930.0 35 977004.0 36 1512724.0 37 2208771.0 38 1743216.0 39 226982.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.18436828230387 19.97889201767983 14.213414723398113 23.62332497661819 2 15.469773224757871 20.281483469960936 40.26287399345843 23.985869311822768 3 18.670947584490605 25.70948492747568 29.837159618088037 25.78240786994568 4 12.863317529421622 16.770692542417706 37.845930534792664 32.52005939336801 5 13.44090307803659 37.7643494165532 34.43004188900564 14.364705616404569 6 31.167046503054618 37.03780699965314 17.60161451741638 14.193531979875853 7 27.87245570759215 32.28013548366848 21.914371358583747 17.93303745015562 8 26.330834156429145 35.562371319268095 19.628026964911445 18.478767559391322 9 26.491953512547877 16.01931762556709 20.588582011596632 36.9001468502884 10-11 25.31311088784583 26.356431198155978 27.52913402522023 20.80132388877796 12-13 25.536868331058148 23.835879127915618 28.098305121349725 22.528947419676506 14-15 23.048275309458536 25.323375061383086 25.691219428802793 25.93713020035559 16-17 22.25800539788622 28.295048856279465 26.604086924859903 22.842858820974413 18-19 22.054552422434245 27.413842387685854 28.77885134284497 21.752753847034935 20-21 22.94600988122927 26.583921774459434 28.691178635860748 21.778889708450553 22-23 22.94180673114835 26.2605904269292 28.487899012086608 22.309703829835847 24-25 22.53901379354505 26.47043374258881 28.716623516898597 22.27392894696754 26-27 22.407425908938155 26.686966209813413 28.48943747609518 22.41617040515325 28-29 22.200853315383316 26.751490249139735 28.728254296158617 22.31940213931833 30-31 22.71762799445788 26.577542271063425 28.607611965347736 22.097217769130957 32-33 22.705436950399093 26.777526856537527 28.24614530928587 22.27089088377751 34-35 22.75743354337605 26.733886997432844 28.511213053080564 21.997466406110544 36-37 22.68724702511707 26.563223842083566 28.413483757144515 22.336045375654848 38-39 22.63358597168258 26.710264925562583 28.488327551085607 22.16782155166923 40-41 22.551078268398346 26.31074098300963 28.79700511921841 22.341175629373613 42-43 22.439041202446543 26.42871744172498 28.434762999025544 22.697478356802932 44-45 22.295021196116384 26.857684199010052 28.38388733837476 22.463407266498812 46-47 22.422602504200935 26.615393497176143 28.06318607423906 22.898817924383856 48-49 22.787330841875193 26.709249920174276 27.882196389103697 22.621222848846834 50-51 22.592083783107107 26.728182277393465 27.835568243624103 22.844165695875326 52-53 22.447276033763405 27.002739563423116 27.666189019126474 22.883795383687 54-55 22.396006170252793 26.89971239936602 27.737807452909635 22.96647397747155 56-57 22.624692318180724 26.833911817373778 27.48963527700674 23.05176058743876 58-59 22.310785319664074 26.85125438325011 27.86670448675156 22.97125581033426 60-61 22.523843150165042 27.097511447366333 27.597778336438882 22.78086706602974 62-63 22.56002950230603 26.93635123730645 27.562568767562155 22.941050492825365 64-65 22.204709714026798 26.747353403273344 27.661359972936868 23.38657690976299 66-67 22.372872595825648 26.705700001923034 27.522872340935635 23.398555061315683 68-69 22.092458071797317 26.85430466918764 27.63557138848118 23.417665870533867 70-71 22.212089116484197 26.560352335304017 27.722082709057133 23.505475839154656 72-73 22.316387445676934 26.84910902526376 27.444168882270326 23.390334646788975 74-75 22.32405213736512 26.99662216921975 27.356493160064243 23.322832533350883 76-77 22.255649213709553 26.863835282229047 27.321178296151 23.559337207910403 78-79 22.153056634424566 26.764024425260242 27.395926048419973 23.686992891895223 80-81 22.042864716913222 27.16320415572966 27.21948226860994 23.574448858747186 82-83 22.40899021791983 26.78469658443482 27.58278129820293 23.223531899442428 84-85 22.413255153230676 26.915830077791814 27.119036029966797 23.551878739010718 86-87 21.968699988588646 27.14780345603977 27.39692523363873 23.486571321732853 88-89 22.074197748878525 26.706262233231143 27.42820521129464 23.791334806595703 90-91 22.226514341409136 26.68232791086263 27.413595334068297 23.677562413659935 92-93 22.199088311865005 26.85699633439358 27.21919676407637 23.724718589665045 94-95 21.831681332197938 26.664855079320027 27.41950988740657 24.083953701075473 96-97 21.940585138382477 26.63093253193925 27.3847776501799 24.04370467949838 98-99 21.713783961875986 26.747010351639 27.28685055348887 24.252355132996144 100 21.77139041144638 26.610298248527485 27.22111034443422 24.397200995591916 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2458.0 1 1760.0 2 1922.0 3 2590.0 4 1855.0 5 1498.0 6 2311.5 7 4386.5 8 6943.0 9 8188.5 10 7372.5 11 6300.5 12 6499.5 13 7259.0 14 8084.0 15 9052.0 16 10044.5 17 10967.0 18 11995.5 19 13090.5 20 14235.0 21 15747.0 22 17937.0 23 20869.5 24 24472.0 25 29142.0 26 36099.5 27 44602.5 28 54561.0 29 69415.0 30 89335.5 31 108970.0 32 133695.0 33 163655.0 34 190823.0 35 216518.0 36 248014.5 37 282189.5 38 298840.5 39 302601.5 40 301175.0 41 298104.5 42 303061.5 43 316138.5 44 333712.5 45 359215.0 46 379755.0 47 390359.0 48 403102.5 49 408382.0 50 403416.5 51 398086.5 52 388579.0 53 374013.0 54 360579.0 55 333332.5 56 298691.0 57 261869.5 58 225296.5 59 196161.5 60 162782.5 61 129986.0 62 103950.0 63 81306.0 64 59799.0 65 41779.0 66 29736.0 67 22018.5 68 16506.0 69 12001.5 70 8916.5 71 6658.0 72 4924.0 73 3586.0 74 2564.0 75 1743.0 76 1124.5 77 650.0 78 377.0 79 208.5 80 132.5 81 82.5 82 51.5 83 31.5 84 23.0 85 17.0 86 11.5 87 8.5 88 6.5 89 4.0 90 1.5 91 2.0 92 2.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029165125574030726 2 0.0016651369272370484 3 5.045869476475905E-5 4 0.022857788728435847 5 5.146786866005422E-4 6 4.036695581180723E-5 7 4.036695581180723E-5 8 0.003693576456780362 9 0.01054586720583464 10-11 0.0010646784595364158 12-13 8.073391162361446E-5 14-15 1.3119260638837352E-4 16-17 3.1284390754150607E-4 18-19 2.5229347382379523E-5 20-21 4.036695581180723E-5 22-23 2.8256869068265064E-4 24-25 3.532108633533133E-5 26-27 5.045869476475905E-5 28-29 9.839445479128013E-4 30-31 0.0025027512603320486 32-33 0.009481188746298224 34-35 0.018412377719660575 36-37 0.021278431582298887 38-39 0.020667881375645302 40-41 0.004576603615163645 42-43 0.005010548390140573 44-45 0.00920366592509205 46-47 0.01194861892029494 48-49 1.2168972040332644 50-51 0.018160084245836778 52-53 0.018765588583013887 54-55 0.009193574186139097 56-57 0.007326602479843012 58-59 0.007866510513825935 60-61 0.004884401653228675 62-63 0.007235776829266447 64-65 0.019078432490555394 66-67 0.028877511013871596 68-69 0.02901374948973645 70-71 0.02646558540411612 72-73 0.022973843726394792 74-75 0.009698161133786689 76-77 0.014461461919579942 78-79 0.008355959853044099 80-81 0.0027197236478205124 82-83 0.014506874744868226 84-85 0.024189898270225486 86-87 0.023145403288594973 88-89 0.032172463782010365 90-91 0.020677973114598257 92-93 0.01333118715684934 94-95 0.006635318361565814 96-97 0.0036532095009685545 98-99 0.015475681684351598 100 0.0346146646086247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9909095.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.432058401083204 #Duplication Level Percentage of deduplicated Percentage of total 1 71.18948622583748 36.61421813110381 2 15.4145438857021 15.856034427109808 3 5.790963032649828 8.935234466812796 4 2.6422022956734015 5.43575611114202 5 1.4711154606036634 3.7831248142252005 6 0.8403934029793283 2.593389754915072 7 0.5842614686590112 2.103483898430104 8 0.40115787137377373 1.6505900054840121 9 0.2781699634042335 1.2876168422910343 >10 1.2727934349143515 11.299710835980074 >50 0.06630549037411836 2.3351581105009434 >100 0.04294531767487087 4.2898786394996415 >500 0.004005722542175048 1.3422499511153196 >1k 0.0015359601494792246 1.2516161624689845 >5k 3.0116865582966357E-5 0.0987160103841541 >10k+ 9.035059674889908E-5 1.1232218385370392 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30795 0.3107751010561509 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25803 0.2603971402030155 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15916 0.160620117175181 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0183477905903615E-5 2 0.0 1.0091738952951808E-5 0.0 0.0 2.0183477905903615E-5 3 0.0 1.0091738952951808E-5 0.0 0.0 2.0183477905903615E-5 4 0.0 1.0091738952951808E-5 0.0 0.0 6.055043371771085E-5 5 0.0 1.0091738952951808E-5 0.0 0.0 6.055043371771085E-5 6 0.0 1.0091738952951808E-5 0.0 0.0 8.073391162361446E-5 7 0.0 1.0091738952951808E-5 0.0 0.0 8.073391162361446E-5 8 0.0 1.0091738952951808E-5 0.0 1.0091738952951808E-5 9.082565057656628E-5 9 0.0 1.0091738952951808E-5 0.0 1.0091738952951808E-5 1.0091738952951808E-4 10-11 1.0091738952951808E-5 1.0091738952951808E-5 0.0 1.0091738952951808E-5 1.0091738952951808E-4 12-13 1.0091738952951808E-5 1.0091738952951808E-5 0.0 1.0091738952951808E-5 1.2110086743542171E-4 14-15 1.0091738952951808E-5 1.0091738952951808E-5 0.0 2.0183477905903615E-5 2.0183477905903616E-4 16-17 1.0091738952951808E-5 1.0091738952951808E-5 0.0 2.0183477905903615E-5 2.8761456015912655E-4 18-19 1.0091738952951808E-5 1.0091738952951808E-5 0.0 5.045869476475904E-5 3.4311912440036145E-4 20-21 1.0091738952951808E-5 1.513760842942771E-5 0.0 1.362384758648494E-4 3.7339434125921694E-4 22-23 1.0091738952951808E-5 2.0183477905903615E-5 0.0 3.986236886415964E-4 4.3899064445340367E-4 24-25 1.0091738952951808E-5 2.0183477905903615E-5 0.0 0.0010697243290128918 5.146786866005422E-4 26-27 1.0091738952951808E-5 2.0183477905903615E-5 0.0 0.0024068797402790063 5.348621645064459E-4 28-29 1.0091738952951808E-5 2.0183477905903615E-5 0.0 0.005974309460147471 5.399080339829217E-4 30-31 1.0091738952951808E-5 2.0183477905903615E-5 0.0 0.01942659748443223 5.600915118888254E-4 32-33 1.0091738952951808E-5 2.0183477905903615E-5 0.0 0.048647227622704195 6.004584677006326E-4 34-35 1.0091738952951808E-5 2.0183477905903615E-5 0.0 0.09481188746298225 6.610089014183434E-4 36-37 1.0091738952951808E-5 2.0183477905903615E-5 0.0 0.16709901358297602 6.660547708948194E-4 38-39 1.513760842942771E-5 3.532108633533133E-5 0.0 0.26782466007238803 6.912841182771989E-4 40-41 2.0183477905903615E-5 4.036695581180723E-5 0.0 0.3757053494794429 7.064217267066266E-4 42-43 2.0183477905903615E-5 4.036695581180723E-5 0.0 0.4807452143712418 7.064217267066266E-4 44-45 2.0183477905903615E-5 6.055043371771085E-5 0.0 0.6113928668561559 7.266052046125302E-4 46-47 2.0183477905903615E-5 6.055043371771085E-5 0.0 0.7660941791354307 7.87155638330241E-4 48-49 2.0183477905903615E-5 6.559630319418676E-5 0.0 0.9332537431521244 8.678895499538555E-4 50-51 2.0183477905903615E-5 8.073391162361447E-5 0.0 1.110101376563652 8.880730278597592E-4 52-53 2.0183477905903615E-5 9.082565057656628E-5 0.0 1.2866714871539733 9.385317226245182E-4 54-55 2.0183477905903615E-5 9.082565057656628E-5 0.0 1.484070946943187 0.0010142197647716568 56-57 2.0183477905903615E-5 9.082565057656628E-5 0.0 1.7090662668992476 0.001044494981630512 58-59 2.0183477905903615E-5 9.587152005304218E-5 0.0 1.961309282028278 0.00105963259005994 60-61 2.0183477905903615E-5 1.05963259005994E-4 0.0 2.218058258599801 0.0010697243290128918 62-63 2.0183477905903615E-5 1.110091284824699E-4 0.0 2.4793636553085827 0.0011807334574953617 64-65 2.0183477905903615E-5 1.110091284824699E-4 0.0 2.7484396910111366 0.0011908251964483133 66-67 2.0183477905903615E-5 1.110091284824699E-4 0.0 3.0489969063774236 0.0012009169354012651 68-69 2.0183477905903615E-5 1.110091284824699E-4 0.0 3.38317475006547 0.0012009169354012651 70-71 2.0183477905903615E-5 1.110091284824699E-4 0.0 3.73630487950716 0.0012816508470248798 72-73 2.0183477905903615E-5 1.110091284824699E-4 0.0 4.08058455388711 0.0015339443208486749 74-75 2.0183477905903615E-5 1.110091284824699E-4 0.0 4.42578257651178 0.0015541277987545785 76-77 2.0183477905903615E-5 1.110091284824699E-4 0.0 4.802582879667618 0.0015642195377075304 78-79 2.0183477905903615E-5 1.110091284824699E-4 0.0 5.211490050302273 0.0015743112766604822 80-81 2.0183477905903615E-5 1.110091284824699E-4 0.0 5.637881158672917 0.0015894488850899098 82-83 2.0183477905903615E-5 1.110091284824699E-4 0.0 6.073213547755875 0.001634861710378193 84-85 2.0183477905903615E-5 1.110091284824699E-4 0.0 6.499392729608506 0.001634861710378193 86-87 2.0183477905903615E-5 1.211008674354217E-4 0.0 6.935073283685342 0.001639907579854669 88 2.0183477905903615E-5 1.211008674354217E-4 0.0 7.2823199293174605 0.0016550451882840966 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16580 0.0 51.99688 1 GTATCAA 29655 0.0 41.16187 1 CAACGCA 37210 0.0 32.061005 5 ATCAACG 37290 0.0 31.97303 3 TATCAAC 38860 0.0 31.06546 2 TCAACGC 39250 0.0 30.49673 4 AACGCAG 39690 0.0 30.364857 6 GTACATG 41285 0.0 27.031628 1 GTACTAG 3715 0.0 26.907503 1 ACGCAGA 44955 0.0 26.51642 7 TACATGG 42930 0.0 25.879845 2 GTGGTAT 7640 0.0 25.737913 1 CGCAGAG 46660 0.0 25.497086 8 ACATGGG 42770 0.0 24.914341 3 TACCTGG 6610 0.0 24.488289 2 CATGGGG 28910 0.0 23.128595 4 GCAGAGT 50915 0.0 23.125433 9 TGGTATC 8180 0.0 23.057571 2 ATGGGGG 19005 0.0 21.450743 5 GAGTACT 28125 0.0 21.438444 12-13 >>END_MODULE