FastQCFastQC Report
Mon 6 Jun 2016
ERR1211319_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211319_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12646873
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT595030.470495750214302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438040.3463622984116311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270690.2140370983404356No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC196830.15563531000904335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA262750.054.173421
GTATCAA528700.044.2899971
TACCCCG7350.037.718745
ACCTGGG23550.037.1118663
CAACGCA629700.036.064145
TCTCTGC75750.035.6680538
ATCAACG638850.035.6283763
GTACTAG107100.035.224511
TATTCTC79000.034.3195235
TCAAGAC13250.034.0444343
TATCAAC685800.033.8069462
TACCTGG26550.033.484352
GCATCGT104400.033.1712048
AACGCAG714900.032.6275256
TAGGCAT134400.032.514435
TATCCGC3200.032.3394621
CATGGGT42200.031.6225324
TTCTCTG90100.030.249257
ACGCAGA754150.030.0206767
CGCAGAG754700.029.9228978
Produced by FastQC (version 0.11.5)