##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211319_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12646873 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.331814986993226 31.0 31.0 34.0 28.0 34.0 2 31.37241308582762 31.0 31.0 34.0 28.0 34.0 3 31.403447634842227 31.0 31.0 34.0 28.0 34.0 4 35.028239470737155 37.0 35.0 37.0 32.0 37.0 5 35.09093394074567 37.0 35.0 37.0 32.0 37.0 6 35.15090172883052 37.0 35.0 37.0 32.0 37.0 7 35.08260619047886 37.0 35.0 37.0 32.0 37.0 8 35.094970907037656 37.0 35.0 37.0 32.0 37.0 9 36.61731069806742 39.0 37.0 39.0 32.0 39.0 10-11 36.55262541973815 39.0 37.0 39.0 32.0 39.0 12-13 36.4689569508605 39.0 37.0 39.0 32.0 39.0 14-15 37.5441043015139 40.0 37.0 41.0 32.0 41.0 16-17 37.56321127760198 40.0 37.0 41.0 32.0 41.0 18-19 37.612963773732844 40.0 37.0 41.0 32.0 41.0 20-21 37.59468550051859 40.0 37.0 41.0 32.0 41.0 22-23 37.45990277596684 40.0 37.0 41.0 31.0 41.0 24-25 37.33998317212484 40.0 37.0 41.0 31.0 41.0 26-27 37.17391990889764 40.0 36.0 41.0 31.0 41.0 28-29 37.06463392176074 39.5 36.0 41.0 30.5 41.0 30-31 36.87928498214539 39.0 36.0 41.0 30.0 41.0 32-33 36.74972817391303 39.0 35.0 41.0 30.0 41.0 34-35 36.615912249613004 39.0 35.0 41.0 30.0 41.0 36-37 36.511606030992795 39.0 35.0 41.0 30.0 41.0 38-39 36.3476921923704 39.0 35.0 41.0 29.0 41.0 40-41 36.171056355195475 39.0 35.0 40.0 28.5 41.0 42-43 36.01566561157054 39.0 35.0 40.0 28.0 41.0 44-45 35.80613998416842 38.0 35.0 40.0 27.0 41.0 46-47 35.572347488584725 38.0 34.5 40.0 26.5 41.0 48-49 35.27673492886345 38.0 34.0 40.0 25.5 41.0 50-51 34.56883638350761 37.5 33.5 39.5 24.0 40.5 52-53 34.78940343593234 38.0 33.5 40.0 25.0 40.5 54-55 35.137084518837185 38.0 34.0 40.0 26.0 41.0 56-57 34.99537692044508 38.0 34.0 40.0 25.5 41.0 58-59 34.73984679849319 37.5 34.0 40.0 25.0 41.0 60-61 34.493184837073954 37.0 33.0 40.0 25.0 41.0 62-63 34.22465952650904 36.5 33.0 40.0 24.0 41.0 64-65 33.96403075289837 36.0 33.0 40.0 23.0 41.0 66-67 33.774040389272514 35.5 33.0 39.0 23.0 41.0 68-69 33.47775552897542 35.0 32.5 39.0 23.0 41.0 70-71 33.23005311273387 35.0 32.0 39.0 23.0 41.0 72-73 32.93574633033794 35.0 32.0 38.0 22.0 40.0 74-75 32.58174526620138 35.0 31.5 37.0 21.0 40.0 76-77 32.27415298627574 35.0 31.0 37.0 20.0 39.0 78-79 31.930807243814343 35.0 31.0 36.0 20.0 39.0 80-81 31.633235385537596 35.0 31.0 36.0 20.0 38.5 82-83 31.317920327024712 35.0 30.5 35.5 19.5 37.0 84-85 31.00965112087391 34.0 30.0 35.0 19.0 37.0 86-87 30.769636336191564 34.0 30.0 35.0 18.0 36.5 88-89 30.530093565421268 34.0 29.5 35.0 18.0 36.0 90-91 30.260773908301285 34.0 29.0 35.0 17.5 36.0 92-93 30.00648796742088 34.0 29.0 35.0 16.5 36.0 94-95 29.807531434845593 34.0 29.0 35.0 15.0 35.0 96-97 29.619148780888366 34.0 29.0 35.0 15.0 35.0 98-99 29.38656444956789 34.0 27.5 35.0 12.0 35.0 100 27.654045154086706 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3391823847564801 1101 2 -0.44089294140447777 1101 3 -0.4121405166110961 1101 4 -0.2904989277571559 1101 5 -0.2775570457022596 1101 6 -0.26507394694135655 1101 7 -0.3231209103054411 1101 8 -0.40942833070709383 1101 9 -0.38980210896737333 1101 10-11 -0.44268088016867324 1101 12-13 -0.4257949209207652 1101 14-15 -0.45689146633831257 1101 16-17 -0.466551156916168 1101 18-19 -0.5381640127488936 1101 20-21 -0.6128960364711489 1101 22-23 -0.556725900389722 1101 24-25 -0.5635200352463983 1101 26-27 -0.5739404055523849 1101 28-29 -0.5832839869486364 1101 30-31 -0.5974251031474154 1101 32-33 -0.6471081823105393 1101 34-35 -0.6701214365323267 1101 36-37 -0.7376915929940111 1101 38-39 -0.710956174440831 1101 40-41 -0.7651457040336211 1101 42-43 -0.78750830822419 1101 44-45 -0.8834524386944125 1101 46-47 -0.7954511561685464 1101 48-49 -0.7496305888324315 1101 50-51 -0.774684578586033 1101 52-53 -0.5582086625950851 1101 54-55 -0.547782582949786 1101 56-57 -0.5366681935316322 1101 58-59 -0.671698949768107 1101 60-61 -0.639945795300541 1101 62-63 -0.5640477727386184 1101 64-65 -0.5904695870105812 1101 66-67 -0.6180357486652994 1101 68-69 -0.5803284108177351 1101 70-71 -0.5720585828608407 1101 72-73 -0.6927778790128727 1101 74-75 -0.7852518097666952 1101 76-77 -0.7038126135991156 1101 78-79 -0.7515373226795177 1101 80-81 -0.7928085674978043 1101 82-83 -0.9062829892738833 1101 84-85 -0.7934350941124535 1101 86-87 -0.8348202596882892 1101 88-89 -0.915879243304385 1101 90-91 -0.8458842965265561 1101 92-93 -0.9662342704159315 1101 94-95 -1.0896567062269717 1101 96-97 -1.065508560577566 1101 98-99 -1.1065366601115088 1101 100 -1.02010022638013 1102 1 -0.3547917801405944 1102 2 -0.3400088831078847 1102 3 -0.3346295020907135 1102 4 -0.18447038230152657 1102 5 -0.17886751824184444 1102 6 -0.221785561667609 1102 7 -0.28536646580908354 1102 8 -0.2529092880039485 1102 9 -0.3418017858744946 1102 10-11 -0.3529015806942155 1102 12-13 -0.36420685441781586 1102 14-15 -0.46572042096156707 1102 16-17 -0.5060883805539191 1102 18-19 -0.5063138696598486 1102 20-21 -0.5224574808213376 1102 22-23 -0.5702336249643878 1102 24-25 -0.5738193208618796 1102 26-27 -0.5119307095151768 1102 28-29 -0.5923105483756075 1102 30-31 -0.6249275214467431 1102 32-33 -0.6204854210426234 1102 34-35 -0.6442118135953052 1102 36-37 -0.7154423597665129 1102 38-39 -0.8401972842081804 1102 40-41 -0.814342637768867 1102 42-43 -0.8254364401484509 1102 44-45 -0.8545968605846923 1102 46-47 -0.8769711230957 1102 48-49 -0.8405998011905993 1102 50-51 -0.7948111016228054 1102 52-53 -0.6483868767408936 1102 54-55 -0.6154492968105743 1102 56-57 -0.6060125229703246 1102 58-59 -0.5865439337491196 1102 60-61 -0.6347885794825459 1102 62-63 -0.6237185214259142 1102 64-65 -0.6766101863437015 1102 66-67 -0.6222709994567595 1102 68-69 -0.6561260036341068 1102 70-71 -0.6806796819009335 1102 72-73 -0.7385978378418727 1102 74-75 -0.7618209035241037 1102 76-77 -0.7732560548616689 1102 78-79 -0.7537650505584601 1102 80-81 -0.8046977684810379 1102 82-83 -0.8499173656723293 1102 84-85 -0.8883875306482736 1102 86-87 -0.9118349940488031 1102 88-89 -1.0657304734559219 1102 90-91 -0.9877026175514239 1102 92-93 -0.9412127235971184 1102 94-95 -0.9547446121212566 1102 96-97 -1.1313037322812995 1102 98-99 -1.0755972579892088 1102 100 -1.0395646549256554 1103 1 -0.4112297661812008 1103 2 -0.41667892577674337 1103 3 -0.4135432750392525 1103 4 -0.29448447358481644 1103 5 -0.269546760737029 1103 6 -0.38292980659480236 1103 7 -0.4131516139006024 1103 8 -0.3543779398568603 1103 9 -0.5170423136348745 1103 10-11 -0.5479350520370616 1103 12-13 -0.6021997291139769 1103 14-15 -0.6909664895414593 1103 16-17 -0.6535511314547051 1103 18-19 -0.6411393706185251 1103 20-21 -0.7222939915247508 1103 22-23 -0.7010117493957395 1103 24-25 -0.6956508149957159 1103 26-27 -0.6144760487893492 1103 28-29 -0.6654126668775788 1103 30-31 -0.6782417207037028 1103 32-33 -0.7606648328612806 1103 34-35 -0.8007521002701168 1103 36-37 -0.8372526946633911 1103 38-39 -0.8220117236912685 1103 40-41 -0.850079704768909 1103 42-43 -0.7859189310761252 1103 44-45 -0.885979768978622 1103 46-47 -0.8778719380465461 1103 48-49 -0.8237480452590376 1103 50-51 -0.8535771450016725 1103 52-53 -0.7013429390750687 1103 54-55 -0.7087409014236457 1103 56-57 -0.7135080783323318 1103 58-59 -0.657034578482353 1103 60-61 -0.5890673812904481 1103 62-63 -0.6334556832933771 1103 64-65 -0.6478159149478273 1103 66-67 -0.6966271322638846 1103 68-69 -0.68450876691724 1103 70-71 -0.6589972279616347 1103 72-73 -0.8092783909619286 1103 74-75 -0.6962658439749632 1103 76-77 -0.6802733561016048 1103 78-79 -0.6941051915935965 1103 80-81 -0.7125834702578508 1103 82-83 -0.8417198698748436 1103 84-85 -0.7708879078867703 1103 86-87 -0.9069150768644292 1103 88-89 -1.0013388289952267 1103 90-91 -0.8775391720525079 1103 92-93 -0.8972897052065605 1103 94-95 -1.1247160271118624 1103 96-97 -1.14324854918965 1103 98-99 -1.1697175270755338 1103 100 -1.1559831815750528 1104 1 -0.40872742032248865 1104 2 -0.4056581216288322 1104 3 -0.44689281395823244 1104 4 -0.31849367882098534 1104 5 -0.26678255682045204 1104 6 -0.27682624934563194 1104 7 -0.3051961311223792 1104 8 -0.30383111842902366 1104 9 -0.43140375224022875 1104 10-11 -0.3978035749892683 1104 12-13 -0.4280899282159112 1104 14-15 -0.5993270049321922 1104 16-17 -0.5671879780733775 1104 18-19 -0.5871553121250628 1104 20-21 -0.5945939444418045 1104 22-23 -0.48731832888282156 1104 24-25 -0.5753718244680286 1104 26-27 -0.6761618887126701 1104 28-29 -0.585600769161168 1104 30-31 -0.6604365838823085 1104 32-33 -0.6847993710571316 1104 34-35 -0.6495768283553858 1104 36-37 -0.6743572346769611 1104 38-39 -0.6617632551585544 1104 40-41 -0.7433206292466323 1104 42-43 -0.6550112153278604 1104 44-45 -0.7293661300976524 1104 46-47 -0.8068447142721098 1104 48-49 -0.7552176308238288 1104 50-51 -0.7250369592533801 1104 52-53 -0.6487728899522978 1104 54-55 -0.6878764432745967 1104 56-57 -0.5703052254732839 1104 58-59 -0.5615109639231193 1104 60-61 -0.4947526673686866 1104 62-63 -0.5577197382228576 1104 64-65 -0.6037656519132639 1104 66-67 -0.5801898585671026 1104 68-69 -0.5463159370897941 1104 70-71 -0.5856330095406648 1104 72-73 -0.6040650513234098 1104 74-75 -0.6685170336748243 1104 76-77 -0.5845124397187895 1104 78-79 -0.611834282549566 1104 80-81 -0.6819268404962706 1104 82-83 -0.7721547338658965 1104 84-85 -0.7943068903717005 1104 86-87 -0.8090999870226518 1104 88-89 -0.7431282913183885 1104 90-91 -0.8247597612160789 1104 92-93 -0.7878864990338812 1104 94-95 -0.7532611538819047 1104 96-97 -0.8361707431616736 1104 98-99 -0.757752644961073 1104 100 -0.9040812342363012 1105 1 -0.3918007654436124 1105 2 -0.459620771340429 1105 3 -0.42338916781026015 1105 4 -0.31140569324276157 1105 5 -0.30229494856463646 1105 6 -0.3098832153829605 1105 7 -0.30078158353488504 1105 8 -0.3300421814937522 1105 9 -0.4515069998967789 1105 10-11 -0.4904291387882793 1105 12-13 -0.4420139771318645 1105 14-15 -0.5918741367257567 1105 16-17 -0.6181932887404216 1105 18-19 -0.55365338998228 1105 20-21 -0.5979217066566349 1105 22-23 -0.6506426692956637 1105 24-25 -0.6064565874278856 1105 26-27 -0.6441632811589457 1105 28-29 -0.6570859579013444 1105 30-31 -0.7255436431329301 1105 32-33 -0.6515637742652345 1105 34-35 -0.739413936188754 1105 36-37 -0.7462186859968938 1105 38-39 -0.7742916185382924 1105 40-41 -0.8689175105789531 1105 42-43 -0.8258955794100729 1105 44-45 -0.8349442168077843 1105 46-47 -0.8088514020512321 1105 48-49 -0.8754874993628619 1105 50-51 -0.830657393177141 1105 52-53 -0.6912865727347466 1105 54-55 -0.6143543466617487 1105 56-57 -0.674506016189099 1105 58-59 -0.6786274938682908 1105 60-61 -0.619427784120667 1105 62-63 -0.7181937686101776 1105 64-65 -0.6314711899885239 1105 66-67 -0.7333382348124005 1105 68-69 -0.7194950281691561 1105 70-71 -0.7096220991328082 1105 72-73 -0.756618658424351 1105 74-75 -0.7242896704272965 1105 76-77 -0.7297071149406023 1105 78-79 -0.6572824295244679 1105 80-81 -0.726570137524341 1105 82-83 -0.7690468920884221 1105 84-85 -0.7610529822032177 1105 86-87 -0.7180653749345076 1105 88-89 -0.6801602261522355 1105 90-91 -0.7336840433635636 1105 92-93 -0.835093871568823 1105 94-95 -0.8320039469528417 1105 96-97 -0.9256312435682581 1105 98-99 -0.9460110109669344 1105 100 -1.0462151507933015 1106 1 -0.4121969549843456 1106 2 -0.4449706471992556 1106 3 -0.4494751830979453 1106 4 -0.30778098392048747 1106 5 -0.2816650768207509 1106 6 -0.3043773315270144 1106 7 -0.31970430774897096 1106 8 -0.27811474326730234 1106 9 -0.469682397974978 1106 10-11 -0.47691871772918404 1106 12-13 -0.45504302476540914 1106 14-15 -0.597174409195901 1106 16-17 -0.5624594537122931 1106 18-19 -0.6155355181546653 1106 20-21 -0.640925444863143 1106 22-23 -0.5616924129102472 1106 24-25 -0.5853015923477116 1106 26-27 -0.5805544169572912 1106 28-29 -0.5997189826245801 1106 30-31 -0.7172926060018128 1106 32-33 -0.6764998662202544 1106 34-35 -0.6183399958764255 1106 36-37 -0.6697188813767241 1106 38-39 -0.6992509309327986 1106 40-41 -0.7669800187323474 1106 42-43 -0.7067084119612375 1106 44-45 -0.7091097207183097 1106 46-47 -0.6933129620790268 1106 48-49 -0.7534910296960291 1106 50-51 -0.7481594324394578 1106 52-53 -0.6076547937586483 1106 54-55 -0.6818452284716088 1106 56-57 -0.6116458352942047 1106 58-59 -0.5779501163186964 1106 60-61 -0.6645629139290108 1106 62-63 -0.6091346885346667 1106 64-65 -0.589016495254775 1106 66-67 -0.6216641013686299 1106 68-69 -0.6622581829586736 1106 70-71 -0.6458754443893397 1106 72-73 -0.5829560210019409 1106 74-75 -0.583320430870792 1106 76-77 -0.5686984332844673 1106 78-79 -0.5271851389215705 1106 80-81 -0.6609287742377674 1106 82-83 -0.6414479080001776 1106 84-85 -0.6027880061733448 1106 86-87 -0.6381473733885095 1106 88-89 -0.6768009681410696 1106 90-91 -0.6521427636772046 1106 92-93 -0.7256283640587746 1106 94-95 -0.833014469988246 1106 96-97 -0.7900908118724139 1106 98-99 -0.8249659934736826 1106 100 -0.8669928742390063 1107 1 -0.5122897924077456 1107 2 -0.506224287090923 1107 3 -0.48200735117446314 1107 4 -0.29978420745676004 1107 5 -0.30035845985928944 1107 6 -0.3446764351342111 1107 7 -0.42109288751134244 1107 8 -0.3660027682402003 1107 9 -0.6055187989746074 1107 10-11 -0.5255778263401325 1107 12-13 -0.5136504075376109 1107 14-15 -0.6588404989564154 1107 16-17 -0.6622509439720545 1107 18-19 -0.5999654295568675 1107 20-21 -0.6633565039926239 1107 22-23 -0.6855347901544491 1107 24-25 -0.7568993365744277 1107 26-27 -0.7410590413340472 1107 28-29 -0.6911011075911162 1107 30-31 -0.7087658335775942 1107 32-33 -0.7454983176426495 1107 34-35 -0.7235877837982372 1107 36-37 -0.7777428765770509 1107 38-39 -0.7964222009127084 1107 40-41 -0.8211671872160906 1107 42-43 -0.7885864761169472 1107 44-45 -0.7348030642042573 1107 46-47 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1.1143861055077409 2310 78-79 1.1069623158989295 2310 80-81 1.114186221740482 2310 82-83 1.1284762647731092 2310 84-85 1.1638846616064278 2310 86-87 1.1379772913739323 2310 88-89 0.9545246578415103 2310 90-91 0.9804788276379277 2310 92-93 1.1792035552772617 2310 94-95 1.2859183311956777 2310 96-97 1.2764680900583087 2310 98-99 1.3276879469568073 2310 100 1.4095261696301833 2311 1 0.6166209692991025 2311 2 0.5521929853049521 2311 3 0.6161442510806587 2311 4 0.49980646113035476 2311 5 0.452031750063675 2311 6 0.33616449870810783 2311 7 0.43486995951825946 2311 8 0.5041859759961795 2311 9 0.6735745577845549 2311 10-11 0.6602532447452276 2311 12-13 0.7437317514161919 2311 14-15 0.9002795339503891 2311 16-17 0.8621907719752357 2311 18-19 0.7685832796355143 2311 20-21 0.806160841424223 2311 22-23 0.8221629514437865 2311 24-25 0.8336685838209661 2311 26-27 0.9315180323553633 2311 28-29 0.9163841148519012 2311 30-31 0.8903730627637074 2311 32-33 0.9274657562870061 2311 34-35 0.9255614409176331 2311 36-37 0.9536901353048677 2311 38-39 1.0017819171364692 2311 40-41 1.0273461331424798 2311 42-43 1.0206744499344538 2311 44-45 1.050481017489794 2311 46-47 1.0519027664029679 2311 48-49 1.0044259335812598 2311 50-51 0.9542629794488349 2311 52-53 0.9033225014577866 2311 54-55 0.9058923973241448 2311 56-57 0.8376702956782438 2311 58-59 0.789904529395308 2311 60-61 0.8281950842979242 2311 62-63 0.8193928318342074 2311 64-65 0.8322414588640896 2311 66-67 0.8492391632227125 2311 68-69 0.8138198151347424 2311 70-71 0.7777852276213153 2311 72-73 0.7778190213990044 2311 74-75 0.8650613367421585 2311 76-77 0.8638560902572934 2311 78-79 0.8776719023246535 2311 80-81 0.9063364040322384 2311 82-83 0.958707686476739 2311 84-85 0.9914421646218479 2311 86-87 0.9665252791796419 2311 88-89 1.0069576112716945 2311 90-91 0.9922208211500312 2311 92-93 1.0179396505639957 2311 94-95 1.0577195752236825 2311 96-97 1.098990984718828 2311 98-99 1.1326236348899137 2311 100 1.1652221202375443 2312 1 0.5440361765391195 2312 2 0.5226006874558422 2312 3 0.5494742867386719 2312 4 0.43986610401616133 2312 5 0.4741962660585557 2312 6 0.49618611613221475 2312 7 0.5265970670631219 2312 8 0.5395258358124622 2312 9 0.692727160005056 2312 10-11 0.6521363909810205 2312 12-13 0.6629884385424205 2312 14-15 0.8680794084267873 2312 16-17 0.8309082126885627 2312 18-19 0.8041512637092367 2312 20-21 0.8575147439527342 2312 22-23 0.8284550297130124 2312 24-25 0.850258052332201 2312 26-27 0.8817227880893199 2312 28-29 0.8945773368554839 2312 30-31 0.9561133876614818 2312 32-33 0.8923954064210093 2312 34-35 0.9632329472722105 2312 36-37 0.9656783284284245 2312 38-39 0.9951371278532548 2312 40-41 0.9926979282716744 2312 42-43 1.0267029890393147 2312 44-45 0.9977088446782787 2312 46-47 1.030333205445686 2312 48-49 0.9945442009414549 2312 50-51 0.9553588968360529 2312 52-53 0.8253657076740453 2312 54-55 0.7917205616226184 2312 56-57 0.7748910533966082 2312 58-59 0.7697146099218344 2312 60-61 0.8226437013933747 2312 62-63 0.8248202116488912 2312 64-65 0.8091781980198078 2312 66-67 0.8368655439470771 2312 68-69 0.9282579742148798 2312 70-71 0.843015363268627 2312 72-73 0.8685470157794271 2312 74-75 0.8417765905384087 2312 76-77 0.8616308002057522 2312 78-79 0.853022896116773 2312 80-81 0.9106724066413392 2312 82-83 0.9237360160179371 2312 84-85 0.9763291716214582 2312 86-87 1.0077073791489575 2312 88-89 1.0295126052400079 2312 90-91 1.0675829830935228 2312 92-93 1.0989209280669883 2312 94-95 1.0812032871879644 2312 96-97 1.1976845936511857 2312 98-99 1.2364409306889854 2312 100 1.2277533066656865 2313 1 0.5458385959039163 2313 2 0.49784378253925254 2313 3 0.5389985731231484 2313 4 0.46333871409082406 2313 5 0.45212337045874307 2313 6 0.4401465652882095 2313 7 0.48849499538273733 2313 8 0.49477043277807553 2313 9 0.5817374513365507 2313 10-11 0.6021632184115902 2313 12-13 0.6493551926737524 2313 14-15 0.8023812934418757 2313 16-17 0.7771786689848525 2313 18-19 0.736503787148699 2313 20-21 0.7791017579149511 2313 22-23 0.6891561442495799 2313 24-25 0.6943758703791545 2313 26-27 0.755396408962163 2313 28-29 0.7943238870496359 2313 30-31 0.8723660143786063 2313 32-33 0.8524858673801603 2313 34-35 0.8713457016608146 2313 36-37 0.9225197698225358 2313 38-39 0.8694783455454242 2313 40-41 0.9193428001297548 2313 42-43 0.7616059961003856 2313 44-45 0.8937901263017025 2313 46-47 0.9370667758232898 2313 48-49 0.9617565290732131 2313 50-51 0.908664335362495 2313 52-53 0.8360505782816432 2313 54-55 0.7689618447945747 2313 56-57 0.753866049132597 2313 58-59 0.7383283303234336 2313 60-61 0.7419186773284636 2313 62-63 0.7686757486979445 2313 64-65 0.7219364282629428 2313 66-67 0.7038029567245658 2313 68-69 0.7241190404969942 2313 70-71 0.7425604124757257 2313 72-73 0.7493193994433298 2313 74-75 0.7723175264009612 2313 76-77 0.7197317537782197 2313 78-79 0.7849510129115949 2313 80-81 0.85901155494264 2313 82-83 0.8238664111278489 2313 84-85 0.8705778895778842 2313 86-87 0.9130519227762761 2313 88-89 0.8702901428758381 2313 90-91 0.9661881748905969 2313 92-93 0.9564557901996551 2313 94-95 0.9513717142820433 2313 96-97 1.0368255262828292 2313 98-99 1.0145254838519264 2313 100 1.0562570312171609 2314 1 0.3416453580254526 2314 2 0.23290910243184015 2314 3 0.33276915712830046 2314 4 0.27317770856478774 2314 5 0.3070903318775464 2314 6 0.3246855768390873 2314 7 0.3919158702616201 2314 8 0.38506948168690514 2314 9 0.5268392356789917 2314 10-11 0.517498335633384 2314 12-13 0.5306627684489911 2314 14-15 0.5981901833018668 2314 16-17 0.62622469127313 2314 18-19 0.6280930868680699 2314 20-21 0.5810437268572741 2314 22-23 0.6461919871015027 2314 24-25 0.6660929757267553 2314 26-27 0.7348680978895459 2314 28-29 0.6172376175887209 2314 30-31 0.6872624357443939 2314 32-33 0.7421684454381179 2314 34-35 0.6922820896640332 2314 36-37 0.6931749108865688 2314 38-39 0.7374152734675263 2314 40-41 0.7755104562437438 2314 42-43 0.6092583287267672 2314 44-45 0.7060913173421071 2314 46-47 0.5734379566224845 2314 48-49 0.48498744552217943 2314 50-51 0.6781522289408599 2314 52-53 0.637386430745444 2314 54-55 0.6450057941482257 2314 56-57 0.5969621712763598 2314 58-59 0.5679025324310629 2314 60-61 0.41629376520545947 2314 62-63 0.5319451600139118 2314 64-65 0.5982228442502375 2314 66-67 0.5854321263007378 2314 68-69 0.6465338986349067 2314 70-71 0.6533519225112414 2314 72-73 0.6843419422379711 2314 74-75 0.6785259017891789 2314 76-77 0.6515857094835766 2314 78-79 0.6997311283180814 2314 80-81 0.7345111761306171 2314 82-83 0.6846004083696471 2314 84-85 0.7548234946187442 2314 86-87 0.7543869346785144 2314 88-89 0.6394904864685422 2314 90-91 0.6446658967418699 2314 92-93 0.8181996708401726 2314 94-95 0.8392674115269223 2314 96-97 0.8565444540372589 2314 98-99 0.8333576993278768 2314 100 0.8506189027854134 2315 1 0.28381786686308175 2315 2 0.27222070747887983 2315 3 0.3085410707024998 2315 4 0.20374213909753536 2315 5 0.18348108790848983 2315 6 0.2042964666106286 2315 7 0.18031477333020973 2315 8 0.17751700934609715 2315 9 0.07392092022585217 2315 10-11 0.24327027623225206 2315 12-13 0.2588353353896693 2315 14-15 0.37026108387878054 2315 16-17 0.15612733714611693 2315 18-19 0.3276508709080659 2315 20-21 0.20634992877165104 2315 22-23 0.17545316636358166 2315 24-25 0.38467568709852884 2315 26-27 0.4128739361194178 2315 28-29 0.3127323255950216 2315 30-31 0.33386640967729164 2315 32-33 0.396274846524328 2315 34-35 0.32689899580307014 2315 36-37 0.4639119479961664 2315 38-39 0.5151939896019471 2315 40-41 0.4998269876631909 2315 42-43 0.45023350498955494 2315 44-45 0.49590396655489144 2315 46-47 0.5762270513567955 2315 48-49 0.4632059814290699 2315 50-51 0.3995269401991308 2315 52-53 0.13060254907026092 2315 54-55 0.3221496849847725 2315 56-57 0.2928513111193567 2315 58-59 0.31784162782972913 2315 60-61 0.39534926194482267 2315 62-63 0.4183681609820695 2315 64-65 0.4262643910793713 2315 66-67 0.3420921561584933 2315 68-69 0.39151676611921005 2315 70-71 0.33335521952117375 2315 72-73 0.37846651065959946 2315 74-75 0.08153701771218635 2315 76-77 0.3551921766676358 2315 78-79 0.4168961074066182 2315 80-81 0.4899108135946477 2315 82-83 0.4400328087437586 2315 84-85 0.4541861515222969 2315 86-87 0.4062198740146563 2315 88-89 0.3751593482141544 2315 90-91 0.5105777057801326 2315 92-93 0.5974797966490257 2315 94-95 0.5611971172034806 2315 96-97 0.5895530341452506 2315 98-99 0.7269515910314581 2315 100 0.7223298550064676 2316 1 0.019430713692404566 2316 2 0.0017867995177809348 2316 3 0.01782145308784777 2316 4 -0.13367053241410076 2316 5 -0.12422868868470971 2316 6 -0.2122236037562999 2316 7 -0.15928489646666577 2316 8 -0.1253915676067976 2316 9 -0.09802269650221263 2316 10-11 -0.06944957546505037 2316 12-13 -0.10016667518935662 2316 14-15 -0.3011900398179179 2316 16-17 -0.12018422642609039 2316 18-19 -0.08951765061565453 2316 20-21 0.003300820051372 2316 22-23 -0.1064694428896118 2316 24-25 -0.12846913566603035 2316 26-27 -0.2480970982616455 2316 28-29 -0.22318296979663899 2316 30-31 -0.8161992667205382 2316 32-33 -0.6407304878507531 2316 34-35 -0.7246800716107344 2316 36-37 -0.5111895037382794 2316 38-39 -0.4519127887344325 2316 40-41 -0.2083448712040692 2316 42-43 -0.20272289011975886 2316 44-45 -0.310239255164241 2316 46-47 -0.5383409051260415 2316 48-49 -0.4319363881314757 2316 50-51 -0.2840451122860159 2316 52-53 -0.5285711502552246 2316 54-55 -0.4868582709383986 2316 56-57 -0.46247402712896957 2316 58-59 -0.761788514909604 2316 60-61 -0.44636337916271174 2316 62-63 -0.43874259498277013 2316 64-65 -0.768426258917529 2316 66-67 -0.9987463483509273 2316 68-69 -1.000498985378293 2316 70-71 -0.8320568692268111 2316 72-73 -1.0335600277398385 2316 74-75 -0.8623315890718182 2316 76-77 -0.5827080907951228 2316 78-79 -0.4821829416847798 2316 80-81 -0.5613424769434623 2316 82-83 -0.4045838653592142 2316 84-85 -0.3620077488699778 2316 86-87 -0.2446917802146089 2316 88-89 -0.22380221536916167 2316 90-91 -0.8703970064081332 2316 92-93 -0.9715061968130954 2316 94-95 -0.56320704804644 2316 96-97 -0.26600701305327235 2316 98-99 -0.21187455995451998 2316 100 -0.291892847324565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 288.0 1 937.0 2 545.0 3 534.0 4 483.0 5 452.0 6 522.0 7 1251.0 8 3168.0 9 5965.0 10 9138.0 11 14805.0 12 21869.0 13 28485.0 14 34140.0 15 39647.0 16 48773.0 17 61448.0 18 76583.0 19 93077.0 20 119030.0 21 149622.0 22 158345.0 23 146403.0 24 135438.0 25 138939.0 26 152415.0 27 174154.0 28 205252.0 29 243157.0 30 295455.0 31 364196.0 32 460351.0 33 602385.0 34 854438.0 35 1169787.0 36 1728538.0 37 2324493.0 38 2146382.0 39 635908.0 40 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.54892224853751 23.483462297581756 13.980931983041337 21.986683470839395 2 14.598502760697246 21.261809790065417 42.178663041678185 21.96102440755915 3 17.134193391545917 30.173858778187373 29.954053060375873 22.73789476989084 4 11.20132495592799 18.286671031623104 42.701373948992014 27.81063006345689 5 11.534002579327938 40.10140790623248 35.751530383579926 12.61305913085965 6 26.15830696154967 41.6741713258312 19.1195800723928 13.047941640226323 7 25.034451453748506 33.627071200831324 25.282984478413695 16.05549286700647 8 25.453054754325162 35.188377802114616 21.06773326878666 18.29083417477356 9 26.588137660599383 15.235633289901212 23.38906265164363 34.78716639785578 10-11 24.004599188046825 27.43493899791346 28.393527677451562 20.16693413658815 12-13 24.61754853123117 25.813554993018293 28.772580007645747 20.796316468104795 14-15 22.7795060618517 26.592810568508746 26.565111269003623 24.062572100635936 16-17 20.044498832257652 29.321871762190067 28.318180097743817 22.315449307808464 18-19 19.493516491404726 28.937569941468173 31.340892763105987 20.228020804021114 20-21 21.136432864935625 28.034116789573616 30.50935763713299 20.320092708357766 22-23 21.885670405749426 27.804234940980443 29.90460628089011 20.405488372380017 24-25 21.08801083030063 28.198596127819386 30.87098546794882 19.842407573931162 26-27 20.76116699619374 28.432736107458012 30.117930752200323 20.688166144147925 28-29 20.055426346255732 28.15819300396446 30.880663584577235 20.905717065202577 30-31 20.904207548696014 28.098701090141304 30.56028726047042 20.436804100692264 32-33 21.289195634996005 28.799738604136277 29.506565075300824 20.404500685566894 34-35 21.19341837239197 28.256327499258383 30.596487689113022 19.953766439236624 36-37 21.016233793329107 27.80512961559544 30.17120696100021 21.007429630075244 38-39 21.192992651233176 28.0057657954701 30.69736293924726 20.103878614049464 40-41 20.646195976284098 27.08693731419044 31.592739469545705 20.674127239979754 42-43 20.63557044463056 27.324724470448285 30.556414391888758 21.483290693032398 44-45 20.495898192869312 28.502153855209755 30.212179679952317 20.789768271968615 46-47 20.745611694494603 28.019340393432472 29.63315495112782 21.6018929609451 48-49 21.538397087232923 27.751727099694897 29.584204857676323 21.12567095539586 50-51 21.42913991763622 27.586122872254492 28.93051531081196 22.05422189929733 52-53 20.492488132973364 28.564843408417985 29.027778898664604 21.91488955994405 54-55 20.91536998745101 28.605998396023878 28.635831165196684 21.842800451328422 56-57 21.584732136916493 27.996479246804707 28.40266492424906 22.016123692029748 58-59 20.551909863108364 27.979849063783675 29.639241596639902 21.828999476468063 60-61 20.842626737188155 29.067101263333335 28.3763238798907 21.713948119587815 62-63 21.281613310284538 28.091444914298826 28.478818973326426 22.148122802090214 64-65 20.20845174005668 28.12526107495794 28.852924450919808 22.813362734065567 66-67 21.187155097428114 27.517789269201113 28.49449916121751 22.800556472153254 68-69 20.36278773357353 27.89665988837879 28.817158107372272 22.92339427067541 70-71 20.79011335329211 27.302669354578068 29.127551855372108 22.779665436757714 72-73 21.485280940880475 27.60674804032684 28.879353757845124 22.028617260947566 74-75 21.30698468602355 28.160812990268973 28.606588382775875 21.925613940931594 76-77 20.869723543903394 27.960905232022043 28.827589305891294 22.341781918183273 78-79 20.648025981613234 27.877046072613442 28.743698518849296 22.731229426924024 80-81 20.375292463519276 28.373527604445915 29.024229388321572 22.226950543713237 82-83 21.517893492034286 27.173470015808178 30.00334881451333 21.30528767764421 84-85 21.376746695697165 27.60635242626733 28.683516382664614 22.33338449537089 86-87 20.19011241395297 28.330044802605087 29.418859657797654 22.06098312564429 88-89 21.302197978916617 26.968717915060946 29.219605270762912 22.50947883525952 90-91 21.000662203394164 27.05883814440307 29.63525561691933 22.305244035283437 92-93 21.397849487891165 27.534886514377632 29.052550383982716 22.01471361374849 94-95 19.912992173307583 26.985771858646167 29.93461573857817 23.16662022946808 96-97 20.69793158565245 27.01607096227953 29.38020763271798 22.90578981935004 98-99 20.14265265017837 27.604153951349737 29.234008139200903 23.01918525927099 100 20.958202684145586 26.816833566426922 28.359027728570595 23.865936020856896 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2457.0 1 1681.0 2 1146.0 3 1403.0 4 1205.5 5 1164.5 6 1909.0 7 3468.5 8 5528.0 9 6624.5 10 6281.0 11 6227.0 12 7254.5 13 8837.5 14 10784.5 15 12871.0 16 14716.5 17 16404.0 18 17969.5 19 19462.5 20 20921.5 21 22428.0 22 24057.5 23 26268.0 24 28247.5 25 30965.0 26 38115.0 27 45898.0 28 52310.0 29 76073.5 30 120592.5 31 162552.5 32 231178.0 33 322121.0 34 405128.5 35 477256.0 36 559426.0 37 662452.5 38 675949.0 39 616468.0 40 536746.0 41 449479.0 42 400462.5 43 393785.5 44 411838.0 45 464910.0 46 500725.5 47 497738.5 48 494284.5 49 464902.0 50 425587.5 51 410318.5 52 398695.0 53 377093.5 54 352907.5 55 296446.5 56 253994.0 57 198756.0 58 139445.5 59 130044.5 60 105364.5 61 67602.0 62 45766.0 63 37111.5 64 26724.0 65 10085.5 66 3622.0 67 2000.5 68 1375.5 69 997.5 70 716.0 71 568.0 72 446.5 73 345.5 74 286.5 75 229.5 76 182.5 77 138.5 78 109.0 79 85.0 80 68.5 81 58.5 82 56.0 83 45.0 84 31.5 85 28.0 86 18.5 87 17.0 88 16.5 89 12.0 90 10.0 91 8.5 92 5.0 93 2.0 94 1.5 95 1.5 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025207812239436578 2 0.11510355168427801 3 0.016367682351202548 4 0.017601188847235202 5 0.01972028975067592 6 0.04615370139322186 7 0.04662812696861904 8 0.038412657421324624 9 0.03286187818917767 10-11 0.03670077180343315 12-13 0.03597727280095246 14-15 0.031264645418673846 16-17 0.037507295281608344 18-19 0.03641611645819484 20-21 0.04697999260370528 22-23 0.04660440568984918 24-25 0.0652967733604979 26-27 0.05767828932891158 28-29 0.04992538471762941 30-31 0.04807907852004207 32-33 0.04356808200730726 34-35 0.044461583507638604 36-37 0.04053571187122698 38-39 0.0456436938996699 40-41 0.05467754756452444 42-43 0.05559477034362565 44-45 0.05886039972094288 46-47 0.06030344417944261 48-49 0.06025204807544126 50-51 0.054171493617434126 52-53 0.06381814698384336 54-55 0.05068841918472654 56-57 0.05506499511776547 58-59 0.05349543717249316 60-61 0.048921183916372055 62-63 0.05553151360023936 64-65 0.053965909201428686 66-67 0.05161750260321266 68-69 0.057330377240286994 70-71 0.057875966651993745 72-73 0.06047344667729327 74-75 0.05081493267149911 76-77 0.05148703556997845 78-79 0.06152509003609035 80-81 0.06249370891919291 82-83 0.05202076434230027 84-85 0.05766642868952665 86-87 0.05521522988330792 88-89 0.06371140122937899 90-91 0.06928194819383415 92-93 0.07373364150964432 94-95 0.0769676425152684 96-97 0.07253176338530481 98-99 0.08203213553263325 100 0.12681395630366493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2646873E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.878757355983414 #Duplication Level Percentage of deduplicated Percentage of total 1 76.97458915597409 17.610829478760433 2 11.637535369799616 5.325046958946403 3 4.246928033514111 2.914933079612794 4 2.0590894454833015 1.8843763118991559 5 1.1610238348540507 1.3281391301069594 6 0.6843734742883631 0.9394568795468887 7 0.4906602711220383 0.7857988101055505 8 0.3524549299584386 0.6450984657151397 9 0.26634857645538873 0.5484352007550992 >10 1.457183715818786 6.373149320719877 >50 0.2127887854012281 3.4790592826991187 >100 0.3243499971643137 16.879276630919435 >500 0.07454790040780561 11.862888666794854 >1k 0.055085511490144366 22.37979367056421 >5k 0.0026065699443285975 3.854851411157346 >10k+ 4.344283240547663E-4 3.188866701696817 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59503 0.470495750214302 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43804 0.3463622984116311 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27069 0.2140370983404356 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 19683 0.15563531000904335 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.907092923286254E-6 0.0 10-11 7.907092923286254E-6 0.0 0.0 7.907092923286254E-6 0.0 12-13 7.907092923286254E-6 0.0 0.0 1.186063938492938E-5 0.0 14-15 7.907092923286254E-6 0.0 0.0 1.5814185846572508E-5 0.0 16-17 7.907092923286254E-6 0.0 0.0 2.7674825231501887E-5 0.0 18-19 7.907092923286254E-6 0.0 0.0 3.1628371693145015E-5 0.0 20-21 7.907092923286254E-6 0.0 0.0 1.3837412615750946E-4 0.0 22-23 7.907092923286254E-6 0.0 0.0 6.641958055560454E-4 0.0 24-25 1.5814185846572508E-5 0.0 0.0 0.0015774650381956078 0.0 26-27 1.5814185846572508E-5 0.0 0.0 0.003427724782244591 0.0 28-29 1.5814185846572508E-5 0.0 0.0 0.007867557458669823 0.0 30-31 1.5814185846572508E-5 0.0 0.0 0.025053623927432496 0.0 32-33 1.5814185846572508E-5 0.0 0.0 0.06369163349707077 0.0 34-35 1.5814185846572508E-5 0.0 0.0 0.12112875649182211 0.0 36-37 1.5814185846572508E-5 0.0 0.0 0.21466571222783687 0.0 38-39 1.5814185846572508E-5 0.0 0.0 0.3485169812332266 0.0 40-41 1.5814185846572508E-5 0.0 0.0 0.49274630970042954 0.0 42-43 1.5814185846572508E-5 0.0 0.0 0.6396007930181635 0.0 44-45 1.5814185846572508E-5 0.0 0.0 0.8120663503144216 0.0 46-47 1.5814185846572508E-5 0.0 0.0 1.0186233387494283 0.0 48-49 1.5814185846572508E-5 0.0 0.0 1.2467271554003903 0.0 50-51 1.5814185846572508E-5 0.0 0.0 1.4903288741809932 0.0 52-53 1.5814185846572508E-5 0.0 0.0 1.7236711398936322 0.0 54-55 1.5814185846572508E-5 3.953546461643127E-6 0.0 1.9681307782564117 0.0 56-57 1.5814185846572508E-5 7.907092923286254E-6 0.0 2.2549961559667757 0.0 58-59 1.5814185846572508E-5 7.907092923286254E-6 0.0 2.5902134069030343 0.0 60-61 1.5814185846572508E-5 7.907092923286254E-6 0.0 2.9306849210868173 0.0 62-63 1.5814185846572508E-5 7.907092923286254E-6 0.0 3.274706719993156 0.0 64-65 1.5814185846572508E-5 7.907092923286254E-6 0.0 3.625682807125524 0.0 66-67 1.5814185846572508E-5 7.907092923286254E-6 0.0 4.007935400315952 0.0 68-69 1.5814185846572508E-5 7.907092923286254E-6 0.0 4.41754653502095 0.0 70-71 1.5814185846572508E-5 7.907092923286254E-6 0.0 4.871915769218209 0.0 72-73 1.5814185846572508E-5 7.907092923286254E-6 0.0 5.329186906518315 0.0 74-75 1.5814185846572508E-5 7.907092923286254E-6 0.0 5.768224287537322 0.0 76-77 1.5814185846572508E-5 7.907092923286254E-6 0.0 6.256902397928721 0.0 78-79 1.5814185846572508E-5 7.907092923286254E-6 0.0 6.767538505368086 0.0 80-81 1.5814185846572508E-5 7.907092923286254E-6 0.0 7.328396513509704 0.0 82-83 1.5814185846572508E-5 7.907092923286254E-6 0.0 7.894156919263757 7.907092923286254E-6 84-85 1.5814185846572508E-5 7.907092923286254E-6 0.0 8.427446847928337 7.907092923286254E-6 86-87 1.5814185846572508E-5 7.907092923286254E-6 0.0 8.983774091824912 7.907092923286254E-6 88 1.5814185846572508E-5 7.907092923286254E-6 0.0 9.413955528769838 7.907092923286254E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 26275 0.0 54.17342 1 GTATCAA 52870 0.0 44.289997 1 TACCCCG 735 0.0 37.71874 5 ACCTGGG 2355 0.0 37.111866 3 CAACGCA 62970 0.0 36.06414 5 TCTCTGC 7575 0.0 35.668053 8 ATCAACG 63885 0.0 35.628376 3 GTACTAG 10710 0.0 35.22451 1 TATTCTC 7900 0.0 34.319523 5 TCAAGAC 1325 0.0 34.044434 3 TATCAAC 68580 0.0 33.806946 2 TACCTGG 2655 0.0 33.48435 2 GCATCGT 10440 0.0 33.171204 8 AACGCAG 71490 0.0 32.627525 6 TAGGCAT 13440 0.0 32.51443 5 TATCCGC 320 0.0 32.339462 1 CATGGGT 4220 0.0 31.622532 4 TTCTCTG 9010 0.0 30.24925 7 ACGCAGA 75415 0.0 30.020676 7 CGCAGAG 75470 0.0 29.922897 8 >>END_MODULE