FastQCFastQC Report
Mon 6 Jun 2016
ERR1211319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211319_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12646873
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT534300.42247597489118455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424140.3353714392482632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251090.19853919621079455No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC182940.14465235793859874No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA159360.12600743282548976No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA278500.055.2876051
TACCCCG6350.050.97565
GTATCAA556450.043.35791
ACCTGGG21600.040.6238753
TCAAGAC16750.040.0603523
TACCTGG24750.036.208062
CCCGGCG8950.035.118458
TCTCTGC75900.035.106718
CAACGCA665800.035.0538985
ATCAACG674050.034.6124653
TCCGCAC4550.034.0326733
TATTCTC79250.033.8005565
CCGCACA4650.033.3006554
TATCAAC731050.032.7318272
AACGCAG752500.031.8751986
TATCCGC3550.031.7270471
GTACTAG104750.031.4058671
TAGGCAT131700.030.099415
TTCTCTG89700.029.6533817
CATGGGT35950.029.3680734
Produced by FastQC (version 0.11.5)