##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211319_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12646873 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16279700128245 33.0 31.0 34.0 30.0 34.0 2 32.30091675626062 34.0 31.0 34.0 30.0 34.0 3 32.35492022415343 34.0 31.0 34.0 30.0 34.0 4 35.98956350712148 37.0 35.0 37.0 35.0 37.0 5 35.90975856245255 37.0 35.0 37.0 35.0 37.0 6 35.926161036012616 37.0 35.0 37.0 35.0 37.0 7 35.84695505363263 37.0 35.0 37.0 35.0 37.0 8 35.83572089322001 37.0 35.0 37.0 35.0 37.0 9 37.44255714436288 39.0 37.0 39.0 34.0 39.0 10-11 37.394762997936326 39.0 37.0 39.0 34.0 39.0 12-13 37.32762758825838 39.0 37.0 39.0 34.0 39.0 14-15 38.53547149560212 40.0 38.0 41.0 34.0 41.0 16-17 38.53925337907639 40.0 38.0 41.0 34.0 41.0 18-19 38.58954478312545 40.0 38.0 41.0 34.0 41.0 20-21 38.57108377699373 40.0 38.0 41.0 34.0 41.0 22-23 38.453048196182564 40.0 38.0 41.0 34.0 41.0 24-25 38.36053303452957 40.0 38.0 41.0 34.0 41.0 26-27 38.232490395056544 40.0 38.0 41.0 34.0 41.0 28-29 38.16432955403285 40.0 37.5 41.0 33.0 41.0 30-31 38.1388196117728 40.0 37.0 41.0 34.0 41.0 32-33 38.04960400092576 40.0 37.0 41.0 33.0 41.0 34-35 37.923983145873294 40.0 37.0 41.0 33.0 41.0 36-37 37.78343156446657 40.0 37.0 41.0 33.0 41.0 38-39 37.657518186511396 40.0 36.0 41.0 33.0 41.0 40-41 37.53045816147596 40.0 36.0 41.0 32.5 41.0 42-43 37.407386988072076 40.0 36.0 41.0 32.0 41.0 44-45 37.18127904028134 40.0 36.0 41.0 31.5 41.0 46-47 36.872726048565525 39.0 35.5 41.0 31.0 41.0 48-49 35.22879363143758 39.0 35.0 40.5 23.0 41.0 50-51 35.93992965691994 38.5 35.0 40.0 28.0 41.0 52-53 36.02818372573204 38.5 35.0 40.0 29.0 41.0 54-55 35.853622116708216 38.0 35.0 40.0 29.0 41.0 56-57 35.60519979919147 38.0 34.5 40.0 28.0 41.0 58-59 35.33791621849923 37.5 34.0 40.0 28.0 41.0 60-61 35.09195881859492 37.0 34.0 40.0 27.5 41.0 62-63 34.770260798855176 37.0 34.0 40.0 27.0 41.0 64-65 34.45384776141897 36.0 33.5 39.0 26.0 41.0 66-67 34.14954989268889 35.5 33.0 39.0 26.0 40.5 68-69 33.843556268810474 35.0 33.0 39.0 26.0 40.0 70-71 33.582156790852565 35.0 33.0 38.0 25.5 40.0 72-73 33.266339315655344 35.0 32.5 37.5 25.0 40.0 74-75 32.930772492140946 35.0 32.0 37.0 25.0 39.0 76-77 31.994656505208837 34.0 30.5 36.0 23.5 39.0 78-79 32.44988172175051 35.0 32.0 36.0 25.0 39.0 80-81 32.35571634980441 35.0 32.0 36.0 25.0 37.5 82-83 32.1032445332534 35.0 32.0 35.5 24.0 37.0 84-85 31.82267790623026 34.0 31.0 35.0 24.0 37.0 86-87 31.581897121920967 34.0 31.0 35.0 24.0 36.0 88-89 31.368690940440377 34.0 31.0 35.0 23.0 36.0 90-91 31.157868747476154 34.0 31.0 35.0 22.5 36.0 92-93 30.959027974741268 34.0 31.0 35.0 20.5 35.0 94-95 30.786501928184144 34.0 30.0 35.0 20.0 35.0 96-97 30.618589037780325 34.0 30.0 35.0 20.0 35.0 98-99 30.42644992165257 34.0 30.0 35.0 19.5 35.0 100 27.938623563310866 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.24061602924697212 1101 2 -0.2646320041355992 1101 3 -0.2875706364186357 1101 4 -0.19185432893783627 1101 5 -0.12783934373449313 1101 6 -0.22821244494154058 1101 7 -0.26588556705362976 1101 8 -0.21927260559105122 1101 9 -0.33375803917343916 1101 10-11 -0.3162192889770097 1101 12-13 -0.36600951618707 1101 14-15 -0.4429255358421429 1101 16-17 -0.48369330708620595 1101 18-19 -0.5152333558950275 1101 20-21 -0.4988210607490302 1101 22-23 -0.5294865441344214 1101 24-25 -0.5164061086599574 1101 26-27 -0.5738408377121758 1101 28-29 -0.5714183580082093 1101 30-31 -0.5297110413121331 1101 32-33 -0.5252391915866212 1101 34-35 -0.5420194640162421 1101 36-37 -0.5206801509719767 1101 38-39 -0.48156850206334667 1101 40-41 -0.5183973116479308 1101 42-43 -0.5334636428410917 1101 44-45 -0.48889996332370345 1101 46-47 -0.49424725317586393 1101 48-49 0.6890957973889655 1101 50-51 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1105 68-69 -0.6459440846718252 1105 70-71 -0.6844066378152078 1105 72-73 -0.6186220772792765 1105 74-75 -0.6799213524127836 1105 76-77 -0.6625525698403116 1105 78-79 -0.554898863066132 1105 80-81 -0.5495876078182462 1105 82-83 -0.49856076250462067 1105 84-85 -0.4978211418391112 1105 86-87 -0.45603499914601997 1105 88-89 -0.5262957003820219 1105 90-91 -0.5907622952148195 1105 92-93 -0.5325586151782993 1105 94-95 -0.5089153435737224 1105 96-97 -0.5562808027354897 1105 98-99 -0.5863033523005114 1105 100 -0.6603372736734947 1106 1 -0.28785850690945836 1106 2 -0.259763795340902 1106 3 -0.29355993976568584 1106 4 -0.18200478040020585 1106 5 -0.2574684980103328 1106 6 -0.3247934096503826 1106 7 -0.29372636263422436 1106 8 -0.3412948636598614 1106 9 -0.4178778515643913 1106 10-11 -0.3937691323643193 1106 12-13 -0.4405284245335608 1106 14-15 -0.5821776293397178 1106 16-17 -0.5390595931499504 1106 18-19 -0.5787494429241846 1106 20-21 -0.600133730362586 1106 22-23 -0.6090445996225853 1106 24-25 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2208 48-49 2.173128266131151 2208 50-51 1.3492268734975994 2208 52-53 1.0565352358339553 2208 54-55 1.0081960904172576 2208 56-57 0.9792317693552874 2208 58-59 0.9841031126828952 2208 60-61 1.02339754738761 2208 62-63 1.007016416257727 2208 64-65 1.0359287344489232 2208 66-67 0.9883890418645507 2208 68-69 1.0169782048271614 2208 70-71 1.0152079311188018 2208 72-73 1.066318312379451 2208 74-75 1.0545057461737812 2208 76-77 1.0083631598988099 2208 78-79 0.8863701258718777 2208 80-81 0.8951496436979056 2208 82-83 0.9113338798366399 2208 84-85 0.9137969855134003 2208 86-87 0.9899466135615107 2208 88-89 1.0192082814636088 2208 90-91 0.9598501646268183 2208 92-93 0.97153988501044 2208 94-95 1.0246358569518002 2208 96-97 0.9940741080825823 2208 98-99 1.0906304304407186 2208 100 1.2967098846269884 2209 1 0.3325720147020377 2209 2 0.37908056792463896 2209 3 0.40337179230354536 2209 4 0.24575359675805686 2209 5 0.33260265748650397 2209 6 0.36659926780428265 2209 7 0.4377979823804168 2209 8 0.45331396120701584 2209 9 0.6213789728976593 2209 10-11 0.5977881264479379 2209 12-13 0.5977665530746705 2209 14-15 0.7610223772754736 2209 16-17 0.7647044945470611 2209 18-19 0.7326096876634125 2209 20-21 0.7429077120234311 2209 22-23 0.7551083853078993 2209 24-25 0.7721063950433731 2209 26-27 0.8122613593088772 2209 28-29 0.8433431398765876 2209 30-31 0.8274022726555259 2209 32-33 0.7853517411890039 2209 34-35 0.8595145276403855 2209 36-37 0.8928356363019603 2209 38-39 0.8716187073650872 2209 40-41 0.8852676008551015 2209 42-43 0.9134155613878292 2209 44-45 0.9224956718469741 2209 46-47 0.963653979587491 2209 48-49 2.1978455461807513 2209 50-51 1.3161191471388207 2209 52-53 1.0622342946013603 2209 54-55 1.0618795914106016 2209 56-57 1.0541022778950122 2209 58-59 1.0525806001574267 2209 60-61 1.0592393327102982 2209 62-63 1.0411170333271116 2209 64-65 1.0418614077667954 2209 66-67 1.0600239635237614 2209 68-69 1.1039984750606564 2209 70-71 1.094679228357549 2209 72-73 1.1057739330861907 2209 74-75 1.087304017481344 2209 76-77 1.0436811715279397 2209 78-79 0.9813219080937685 2209 80-81 0.9857340609712182 2209 82-83 0.9341015752388131 2209 84-85 1.000760527885845 2209 86-87 1.0829402502399752 2209 88-89 1.0220136393854808 2209 90-91 1.094938439967084 2209 92-93 1.052212484332852 2209 94-95 1.0125009726391774 2209 96-97 1.0414605328091895 2209 98-99 1.1511020365947573 2209 100 1.405641964014503 2210 1 0.5379604514219238 2210 2 0.46906038347982815 2210 3 0.4449459886022282 2210 4 0.2934173566460885 2210 5 0.3587296755916043 2210 6 0.37534898800892336 2210 7 0.4220765162400255 2210 8 0.41093367246473633 2210 9 0.5615018900525612 2210 10-11 0.5699321938464124 2210 12-13 0.5885675278365099 2210 14-15 0.7215351201174727 2210 16-17 0.7170038753903967 2210 18-19 0.6901460402674573 2210 20-21 0.683394771258321 2210 22-23 0.7365501817389557 2210 24-25 0.7566799748901119 2210 26-27 0.7867811225613721 2210 28-29 0.7780053177201367 2210 30-31 0.8003561270163644 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1.0870997400131515 2210 100 1.3567204181000037 2211 1 0.5325049878070587 2211 2 0.4987469364236006 2211 3 0.4718767828758246 2211 4 0.30033893763979336 2211 5 0.3722175225166495 2211 6 0.4142898105840658 2211 7 0.4310855926799775 2211 8 0.44704231747918755 2211 9 0.6050883048480742 2211 10-11 0.6217933954271828 2211 12-13 0.6047531177079506 2211 14-15 0.7439449845242478 2211 16-17 0.6939937550541515 2211 18-19 0.6934509745243318 2211 20-21 0.6673683296336179 2211 22-23 0.6814086746395773 2211 24-25 0.7466591723315048 2211 26-27 0.778220167004811 2211 28-29 0.7888833295985833 2211 30-31 0.799255085169797 2211 32-33 0.801660706796568 2211 34-35 0.8006190708166585 2211 36-37 0.8325361951780721 2211 38-39 0.8336462111664176 2211 40-41 0.8532782146312599 2211 42-43 0.7596485220081348 2211 44-45 0.78146361647417 2211 46-47 0.9117540919576186 2211 48-49 2.153024399344197 2211 50-51 1.3005756152341803 2211 52-53 1.0182616124845651 2211 54-55 0.994267732056727 2211 56-57 0.9456632900004394 2211 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1.1699537965120683 2311 1 0.33411074448696354 2311 2 0.34152797472119545 2311 3 0.37966168879074047 2311 4 0.24233859718634676 2311 5 0.2982137826848046 2311 6 0.3152991907921887 2311 7 0.3624713909615451 2311 8 0.1474059712418807 2311 9 0.40343302119568136 2311 10-11 0.33796238781388865 2311 12-13 0.42390759399546596 2311 14-15 0.40002388603397065 2311 16-17 0.4202226119159107 2311 18-19 0.5559776633540778 2311 20-21 0.6071509583043735 2311 22-23 0.5914499311482828 2311 24-25 0.540670023669442 2311 26-27 0.5900877159543185 2311 28-29 0.61910373568859 2311 30-31 0.5052509530546203 2311 32-33 0.5065480856971263 2311 34-35 0.6484707895659412 2311 36-37 0.6845061048728738 2311 38-39 0.6862366413232692 2311 40-41 0.6507052881218343 2311 42-43 0.6858145712117434 2311 44-45 0.6921155430739816 2311 46-47 0.7508482441502409 2311 48-49 -6.160584419108034 2311 50-51 -1.1780067155321206 2311 52-53 0.23360361139955899 2311 54-55 0.6959290230886666 2311 56-57 0.7815576925233074 2311 58-59 0.7904959345804343 2311 60-61 0.6264227258964254 2311 62-63 0.5338875914033281 2311 64-65 0.5904684072414099 2311 66-67 0.7149153349749042 2311 68-69 0.7051426983655631 2311 70-71 0.7419818979612458 2311 72-73 0.30048399205762877 2311 74-75 0.5923382026755561 2311 76-77 0.640724204951642 2311 78-79 0.6018096268849291 2311 80-81 0.6484287749631079 2311 82-83 0.6899783662236061 2311 84-85 0.6745857780269375 2311 86-87 0.694619321562211 2311 88-89 0.726444369748247 2311 90-91 0.8034270555536871 2311 92-93 0.756095840622141 2311 94-95 0.8036773705684936 2311 96-97 0.8516140565065058 2311 98-99 0.8619588571679131 2311 100 1.0165827833059744 2312 1 0.05188708353604454 2312 2 0.22132089395724108 2312 3 0.2278744159908186 2312 4 -0.017314434980413296 2312 5 0.20073051961234967 2312 6 0.16904381139722346 2312 7 0.29776045041732147 2312 8 0.3391067705701616 2312 9 0.2627739869088046 2312 10-11 0.26518042251609586 2312 12-13 0.2875191643294457 2312 14-15 0.36218542865694303 2312 16-17 0.5123776365468373 2312 18-19 0.33671635954863177 2312 20-21 0.397049396428244 2312 22-23 0.6003301637995051 2312 24-25 0.686814873981838 2312 26-27 0.6429617299078387 2312 28-29 0.7754057447358491 2312 30-31 0.804878170861322 2312 32-33 0.8066395668798236 2312 34-35 0.3933420781984438 2312 36-37 0.6761252599130856 2312 38-39 0.7866109172065165 2312 40-41 0.6379400806642508 2312 42-43 0.6799977068988099 2312 44-45 0.8088738864723695 2312 46-47 0.8093044735109132 2312 48-49 -4.915754563358448 2312 50-51 -0.909998576864119 2312 52-53 0.4679253806882784 2312 54-55 0.7643985162372928 2312 56-57 0.8741061581674359 2312 58-59 0.8504672955717112 2312 60-61 0.8095110341379197 2312 62-63 0.6094569997230863 2312 64-65 0.8215287837490379 2312 66-67 0.8157820169026735 2312 68-69 0.7253707821885627 2312 70-71 0.7356907535586927 2312 72-73 0.8369206261334696 2312 74-75 0.49934671912242123 2312 76-77 0.7519320844100328 2312 78-79 0.4417208927604861 2312 80-81 0.6390234136782027 2312 82-83 0.6698047496780504 2312 84-85 0.7058461554962463 2312 86-87 0.8226607788051332 2312 88-89 0.8121771137348723 2312 90-91 0.6296726081874766 2312 92-93 0.769898606250436 2312 94-95 0.8494855306478648 2312 96-97 0.9062760749544267 2312 98-99 0.9853489032975489 2312 100 0.5413638844591766 2313 1 0.0412877439463486 2313 2 0.16441719346276784 2313 3 0.250450114362998 2313 4 0.01744915096941213 2313 5 0.11144192275795461 2313 6 0.2223840750060404 2313 7 0.11294195186889766 2313 8 0.22425398397987806 2313 9 0.4057463643300423 2313 10-11 0.21312405682527924 2313 12-13 0.017407264072424766 2313 14-15 0.08944868753415136 2313 16-17 0.08764735037136262 2313 18-19 0.3845717457373681 2313 20-21 0.3923012317277639 2313 22-23 0.4620158055158399 2313 24-25 0.23873833590613458 2313 26-27 0.38052484318604485 2313 28-29 0.34187240427801413 2313 30-31 0.5385380755044125 2313 32-33 0.4143596743820126 2313 34-35 0.40249443527467577 2313 36-37 -0.3417653335251529 2313 38-39 0.09806719509386141 2313 40-41 0.3039065459304169 2313 42-43 0.46350491539524086 2313 44-45 0.4062755912942322 2313 46-47 0.30690719424470103 2313 48-49 -5.22543114881956 2313 50-51 -1.3062841866443904 2313 52-53 0.010123436969834643 2313 54-55 0.1830314786827998 2313 56-57 0.17259222116274486 2313 58-59 0.2778306338244434 2313 60-61 0.5962397112958229 2313 62-63 0.6494274093610883 2313 64-65 0.7154382145441076 2313 66-67 0.7587512837066015 2313 68-69 0.7951231852816676 2313 70-71 0.7470258780887562 2313 72-73 0.572395432546493 2313 74-75 0.6324119143542717 2313 76-77 0.6673149217478063 2313 78-79 0.6316810439848979 2313 80-81 -0.23987237698448638 2313 82-83 0.16811429451642113 2313 84-85 0.5747059731141348 2313 86-87 0.6374572056073298 2313 88-89 0.672213076308676 2313 90-91 0.688561145028924 2313 92-93 0.7690753129778969 2313 94-95 0.6476460868038352 2313 96-97 0.6658372941116646 2313 98-99 0.9013566985717283 2313 100 0.9392342604920927 2314 1 0.053264049600073804 2314 2 0.18164293517724417 2314 3 0.2047842047983437 2314 4 0.08225730498146788 2314 5 0.17638506348057348 2314 6 0.2898159239628413 2314 7 0.11472448864640228 2314 8 0.23436168315556216 2314 9 0.4463682752931035 2314 10-11 0.2994348699790521 2314 12-13 0.17934106203298938 2314 14-15 0.2296314565851887 2314 16-17 0.43856317467966477 2314 18-19 0.4833058620765982 2314 20-21 0.551362666672901 2314 22-23 0.273733369317668 2314 24-25 0.3721985094850311 2314 26-27 0.49740236477168054 2314 28-29 0.6075533286380761 2314 30-31 0.5140839914337363 2314 32-33 0.47587500515007264 2314 34-35 0.3633918600753816 2314 36-37 0.41087962249069676 2314 38-39 0.4921089685704345 2314 40-41 0.2696795727004968 2314 42-43 0.25693009963199387 2314 44-45 0.48754099822973274 2314 46-47 0.6018066751725328 2314 48-49 -6.289928461917036 2314 50-51 -1.530384163299395 2314 52-53 -0.877820179834842 2314 54-55 -0.4212501738364338 2314 56-57 -0.15274190159185252 2314 58-59 0.3538416127870718 2314 60-61 0.5897589998345225 2314 62-63 0.4426131752466702 2314 64-65 0.6143457366733074 2314 66-67 0.7218901912448601 2314 68-69 0.7052025601335643 2314 70-71 0.45912741408473323 2314 72-73 0.7008359261993391 2314 74-75 0.693003913835831 2314 76-77 0.6255446449386746 2314 78-79 0.4744729927493694 2314 80-81 0.5636247636846008 2314 82-83 0.5282477568060884 2314 84-85 0.5355145782347108 2314 86-87 0.6229405876530585 2314 88-89 0.5552043201032895 2314 90-91 0.7062071279058024 2314 92-93 0.7160086746239003 2314 94-95 0.8019996009510315 2314 96-97 0.8154548959312677 2314 98-99 0.8872529737411377 2314 100 -0.6100224885381422 2315 1 0.14852875062726412 2315 2 0.2045945743023907 2315 3 0.3009661276802902 2315 4 0.1445970695110006 2315 5 0.21650790412486742 2315 6 0.27401477870230906 2315 7 0.30848525769648916 2315 8 0.26363695578383073 2315 9 0.40942390535417417 2315 10-11 0.2100157089915058 2315 12-13 0.39128373729980837 2315 14-15 0.16627904158171702 2315 16-17 0.3134096711674701 2315 18-19 0.41553496282839575 2315 20-21 0.35936161811564205 2315 22-23 0.41407268884094606 2315 24-25 0.4141247289789689 2315 26-27 0.5174683635235979 2315 28-29 0.5712483376541826 2315 30-31 0.5529395709136296 2315 32-33 0.6048416988188876 2315 34-35 0.6070516401801314 2315 36-37 0.49984889025947155 2315 38-39 0.5706601871916988 2315 40-41 0.6220381081164419 2315 42-43 0.6773719102219218 2315 44-45 0.685866049627279 2315 46-47 0.5635153573211866 2315 48-49 -5.197006597223709 2315 50-51 -1.0271548681951614 2315 52-53 0.19183245629799472 2315 54-55 0.18515996338809515 2315 56-57 0.4813193547806236 2315 58-59 0.5780268561003226 2315 60-61 0.46743003391330973 2315 62-63 0.16638584641250276 2315 64-65 0.39693531501411883 2315 66-67 -0.34917051810322164 2315 68-69 0.3880684702445478 2315 70-71 0.3632778766457676 2315 72-73 0.5157112715239691 2315 74-75 0.659817466348251 2315 76-77 0.6457532191012483 2315 78-79 0.5380532887911329 2315 80-81 0.44597453184670144 2315 82-83 0.457317842363949 2315 84-85 0.4648763478282234 2315 86-87 0.5152901981712397 2315 88-89 0.4886159078770582 2315 90-91 0.5413396535167436 2315 92-93 0.6416341685279257 2315 94-95 0.2841749271106657 2315 96-97 0.3761357172200448 2315 98-99 0.3317374139487157 2315 100 0.4385986654288452 2316 1 0.22928765370793514 2316 2 0.25053053292973004 2316 3 0.14942800252246968 2316 4 -0.09266705057029156 2316 5 0.13879136045523666 2316 6 0.13496985815160656 2316 7 0.21352427554429454 2316 8 0.2981359733695683 2316 9 0.4501845362935484 2316 10-11 0.3867852861525023 2316 12-13 0.3147049508296078 2316 14-15 0.412207504678058 2316 16-17 0.3585056528299546 2316 18-19 0.07054802504041646 2316 20-21 0.45999265076883944 2316 22-23 0.5441818348767598 2316 24-25 0.5697236219056947 2316 26-27 0.5776864157382349 2316 28-29 0.6144309001397019 2316 30-31 0.5525678392149942 2316 32-33 0.5089313782389127 2316 34-35 0.4809832538424814 2316 36-37 0.4212511808493318 2316 38-39 0.4997039839679758 2316 40-41 0.0983774173682832 2316 42-43 0.014634404605828877 2316 44-45 0.24162184678199594 2316 46-47 0.2866923897705078 2316 48-49 -5.235172921612968 2316 50-51 -1.2021661070498624 2316 52-53 0.17325243634100929 2316 54-55 0.4894994627488103 2316 56-57 0.3576499803209998 2316 58-59 0.21951418487741137 2316 60-61 0.3393164847249679 2316 62-63 0.2839319650266674 2316 64-65 0.2894019710056668 2316 66-67 0.3551613102755482 2316 68-69 0.019714604338382458 2316 70-71 -0.388138532193139 2316 72-73 -0.23393843954829663 2316 74-75 -0.014380666187385316 2316 76-77 0.0022445577098046954 2316 78-79 0.1474598181870519 2316 80-81 0.11098299235791842 2316 82-83 0.008625791424542228 2316 84-85 0.07639218627387478 2316 86-87 0.13456081082595972 2316 88-89 -0.04611974195660551 2316 90-91 0.03430584944925741 2316 92-93 0.1639337752290828 2316 94-95 0.3433406622956703 2316 96-97 0.35707336706126114 2316 98-99 0.3517842127129178 2316 100 -0.43115491482541657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 14.0 11 26.0 12 60.0 13 266.0 14 1067.0 15 3227.0 16 8509.0 17 18276.0 18 32170.0 19 49083.0 20 72900.0 21 106989.0 22 127015.0 23 114979.0 24 100978.0 25 103757.0 26 122368.0 27 150077.0 28 185945.0 29 229998.0 30 284440.0 31 356452.0 32 452369.0 33 601407.0 34 872166.0 35 1223337.0 36 1947424.0 37 2781754.0 38 2301536.0 39 398278.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.30111914521837 24.05438501272083 14.683285873763607 21.961209968297187 2 14.408212280576766 21.523061377422476 42.21972019245299 21.849006149547773 3 16.764863582419267 30.194237039102255 30.63730329416764 22.40359608431084 4 11.132681691939204 18.74442610602349 42.60518465861906 27.51770754341824 5 11.378368795784295 40.24147128397024 35.86485565839401 12.515304261851453 6 26.15107757518127 41.864834982450596 18.91788693595022 13.066200506417914 7 24.834753579344138 33.8430141212806 25.21255456883798 16.10967773053728 8 25.396525793233458 35.66205716436534 20.778090736133933 18.16332630626727 9 26.284802525977096 15.453413616571568 23.414791687391986 34.846992170059345 10-11 24.01247785099214 27.635296051650982 28.296652834104375 20.055573263252498 12-13 24.644157593166145 25.83890517291037 28.80382400194262 20.713113231980866 14-15 22.716510620643163 26.73229509641388 26.580486621319693 23.970707661623265 16-17 20.005670993500814 29.413182640307667 28.270392843604718 22.310753522586797 18-19 19.460776546168887 28.85697348719905 31.42305860294941 20.259191363682653 20-21 21.147483294172755 27.91578019823815 30.623096587733894 20.313639919855202 22-23 21.791252060494788 27.710663081792518 30.04878846820325 20.449296389509446 24-25 21.033869290736337 28.11449900704676 30.96597146036812 19.885660241848782 26-27 20.699167141191513 28.363044855041704 30.24516398791906 20.692624015847727 28-29 20.007817042306677 28.12367184015279 31.064361553783836 20.804149563756695 30-31 20.869845961005296 27.940662495933672 30.722787532841007 20.46670401022003 32-33 21.341788046694482 28.675516324844867 29.66476966365149 20.31792596480916 34-35 21.189061702839602 28.182229715691996 30.72564170435963 19.903066877108774 36-37 21.00989490890518 27.71554794268115 30.34367155912831 20.930885589285356 38-39 21.082624237127266 27.803354389979805 31.014717718799062 20.099303654093866 40-41 20.454224572641287 26.988075640255726 31.94098442815741 20.616715358945576 42-43 20.608753665824807 27.15969043346542 30.819070615605526 21.412485285104243 44-45 20.399101816496305 28.337111481486005 30.45168432339556 20.81210237862212 46-47 20.772723410374784 27.805237304680126 29.784505857218903 21.637533427726186 48-49 21.736693877402363 27.621156053831363 29.68784639036005 20.954303678406223 50-51 21.45290203248525 27.464819085569463 29.030384507372297 22.05189437457299 52-53 20.40571048803325 28.426255452098232 29.16049619288803 22.00753786698049 54-55 20.939704580700123 28.52309249786113 28.632355047218866 21.904847874219882 56-57 21.635883769393295 27.817647877036162 28.47666628525275 22.069802068317795 58-59 20.35176218158788 27.974587485118104 29.71776476433926 21.955885568954763 60-61 20.810073746152902 29.07804696407677 28.372021708622945 21.739857581147376 62-63 21.293575799059912 28.040793492709142 28.458093481950748 22.2075372262802 64-65 20.16754462501856 28.15338103432015 28.718425974201605 22.960648366459676 66-67 21.027517500728663 27.6190822701335 28.550871249766175 22.80252897937166 68-69 20.28289862133687 27.897187742351626 28.832152381012644 22.98776125529886 70-71 20.702100035947737 27.216909594012034 29.188039883401203 22.89295048663903 72-73 21.38239022605715 27.615547871308266 28.80888573620593 22.193176166428653 74-75 21.211565625038055 28.285711935950133 28.509275050084433 21.993447388927382 76-77 20.827068831628896 28.10349803659448 28.610374532814113 22.459058598962507 78-79 20.627257053691263 28.004117581587046 28.5595561678814 22.809069196840287 80-81 20.28476550903113 28.637611141824458 28.70388634082105 22.373737008323364 82-83 21.522388046717772 27.37859020985812 29.680022424681763 21.418999318742344 84-85 21.513634099124285 27.80080329314917 28.304813075726308 22.380749532000237 86-87 20.190015524593893 28.621857543086897 29.08578419683499 22.10234273548422 88-89 21.15886782862539 27.348105039010072 28.8536890202834 22.639338112081127 90-91 20.866110486338734 27.307071908807227 29.318265139073485 22.50855246578055 92-93 21.34899826383974 27.862936497530356 28.599341597163054 22.18872364146685 94-95 19.880084039069043 27.281576982739946 29.458455041557336 23.379883936633675 96-97 20.647333249962944 27.423819779523406 28.922589011959275 23.006257958554375 98-99 20.022133875466185 28.085122418409803 28.821061124308823 23.07168258181519 100 20.695110295650196 27.280331590538744 28.12751659325066 23.897041520560396 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2304.0 1 1502.0 2 775.5 3 871.0 4 769.0 5 812.0 6 1379.5 7 2772.5 8 4693.5 9 5757.5 10 5436.5 11 5119.5 12 5936.5 13 7431.5 14 9189.5 15 11255.5 16 13515.0 17 15798.0 18 17977.5 19 19957.0 20 21983.5 21 23783.0 22 25513.0 23 28091.0 24 29979.0 25 32490.5 26 39516.0 27 46950.5 28 53620.0 29 77992.0 30 121880.5 31 163500.5 32 232720.5 33 323883.0 34 406669.0 35 479990.5 36 563693.0 37 665953.5 38 677267.5 39 615150.0 40 531788.0 41 441342.0 42 395521.5 43 391309.0 44 412185.0 45 472747.5 46 509413.5 47 504821.5 48 505192.0 49 475019.5 50 429899.5 51 404336.5 52 388836.0 53 366450.5 54 346865.0 55 298702.0 56 255293.0 57 199561.5 58 140928.0 59 133606.5 60 106548.5 61 64152.5 62 41525.5 63 33883.0 64 23031.5 65 6746.0 66 1513.0 67 817.5 68 589.0 69 418.5 70 288.0 71 207.5 72 170.5 73 128.5 74 91.5 75 66.0 76 49.5 77 30.0 78 20.0 79 13.0 80 9.5 81 6.0 82 6.5 83 6.0 84 2.0 85 1.5 86 0.5 87 1.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030292072989109638 2 0.001992587416668136 3 4.744255753971753E-5 4 0.024583151898496965 5 5.455894117067516E-4 6 4.744255753971753E-5 7 2.3721278769858765E-5 8 0.004214480528111574 9 0.01036619882242828 10-11 0.0011386213809532206 12-13 1.3837412615750946E-4 14-15 1.304670332342232E-4 16-17 4.6651848247388904E-4 18-19 7.907092923286254E-6 20-21 1.0674575446436443E-4 22-23 2.530269735451601E-4 24-25 2.767482523150189E-5 26-27 9.093156861779192E-5 28-29 0.001154435566799793 30-31 0.002648876129300895 32-33 0.009682235284564019 34-35 0.018834695343267857 36-37 0.022100324720585082 38-39 0.02155473530887833 40-41 0.0048826298801292614 42-43 0.005329380630294935 44-45 0.00891524727100525 46-47 0.011817150373851306 48-49 1.2192223326667389 50-51 0.01821794209525153 52-53 0.018300966570946035 54-55 0.009377812207017498 56-57 0.007464295719582225 58-59 0.008353843673451928 60-61 0.005171238771829211 62-63 0.007389178336811004 64-65 0.019613543996211555 66-67 0.03026835171033978 68-69 0.03026835171033978 70-71 0.027817152904121043 72-73 0.023472205342775245 74-75 0.009702003016872233 76-77 0.014861381149316514 78-79 0.008701755762076524 80-81 0.0028663211846912673 82-83 0.014845566963469942 84-85 0.025065484566817425 86-87 0.024227332716949084 88-89 0.03455399607476093 90-91 0.021447989554413963 92-93 0.013655549478515362 94-95 0.006721028984793316 96-97 0.003925871636411625 98-99 0.01583000003241908 100 0.03585075931417987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2646873E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.56129991056688 #Duplication Level Percentage of deduplicated Percentage of total 1 76.04720949064067 16.396766911894115 2 12.334707906384889 5.319046729576102 3 4.330816047185875 2.801340709526113 4 2.060181131543757 1.7768073298922389 5 1.1525517121213669 1.2425256563743066 6 0.7392086289099489 0.9562979366643808 7 0.5030739797334765 0.7592850267965151 8 0.3271001347494452 0.5642163284895703 9 0.2512309844686586 0.48751799426601455 >10 1.5191057757330424 6.294999973901329 >50 0.23311800885147319 3.6129850067470692 >100 0.35848641067145404 17.662081770359077 >500 0.0809167417702181 12.21241402788708 >1k 0.05908206541952259 22.915807072231082 >5k 0.0026605277906306757 3.726354305736402 >10k+ 5.504540256477259E-4 3.271553219658691 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53430 0.42247597489118455 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42414 0.3353714392482632 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25109 0.19853919621079455 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 18294 0.14465235793859874 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 15936 0.12600743282548976 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.907092923286254E-6 0.0 10-11 0.0 0.0 0.0 7.907092923286254E-6 0.0 12-13 0.0 0.0 0.0 1.186063938492938E-5 0.0 14-15 0.0 0.0 0.0 1.5814185846572508E-5 0.0 16-17 0.0 0.0 0.0 2.7674825231501887E-5 0.0 18-19 0.0 0.0 0.0 3.1628371693145015E-5 0.0 20-21 0.0 0.0 0.0 1.2651348677258006E-4 0.0 22-23 0.0 0.0 0.0 6.365209803245436E-4 0.0 24-25 0.0 0.0 0.0 0.0015497902129641057 0.0 26-27 0.0 0.0 0.0 0.003411910596398019 0.0 28-29 0.0 0.0 0.0 0.00788732519097804 0.0 30-31 0.0 0.0 0.0 0.025132694856665357 0.0 32-33 0.0 0.0 0.0 0.0638853572736913 0.0 34-35 0.0 0.0 0.0 0.1218127200296864 0.0 36-37 0.0 0.0 0.0 0.2150175778629231 0.0 38-39 0.0 0.0 0.0 0.34429064006573007 0.0 40-41 0.0 0.0 0.0 0.4830680279623272 0.0 42-43 0.0 0.0 0.0 0.6239012600189786 0.0 44-45 0.0 0.0 0.0 0.7895074142042859 0.0 46-47 0.0 0.0 0.0 0.9876828841406093 0.0 48-49 0.0 0.0 0.0 1.2088165983796944 0.0 50-51 0.0 0.0 0.0 1.4523511068704493 0.0 52-53 0.0 0.0 0.0 1.6854561597953897 0.0 54-55 0.0 0.0 0.0 1.9301213825741748 0.0 56-57 0.0 0.0 0.0 2.2170816453996176 0.0 58-59 0.0 0.0 0.0 2.552607272959885 3.953546461643127E-6 60-61 0.0 0.0 0.0 2.8934780953362935 7.907092923286254E-6 62-63 0.0 0.0 0.0 3.2378478063312564 7.907092923286254E-6 64-65 0.0 0.0 0.0 3.588851568288857 7.907092923286254E-6 66-67 0.0 0.0 0.0 3.9716576579839145 7.907092923286254E-6 68-69 0.0 0.0 0.0 4.382067409074164 7.907092923286254E-6 70-71 0.0 0.0 0.0 4.8369071153003595 7.907092923286254E-6 72-73 0.0 0.0 0.0 5.2949926831715635 7.907092923286254E-6 74-75 0.0 0.0 0.0 5.734935426330288 7.907092923286254E-6 76-77 0.0 0.0 0.0 6.225028906354954 7.907092923286254E-6 78-79 0.0 0.0 0.0 6.736740378431886 7.907092923286254E-6 80-81 0.0 0.0 0.0 7.298440491969833 7.907092923286254E-6 82-83 0.0 0.0 0.0 7.8660076684568585 7.907092923286254E-6 84-85 0.0 0.0 0.0 8.401634143080269 7.907092923286254E-6 86-87 0.0 0.0 0.0 8.960906779090767 7.907092923286254E-6 88 0.0 0.0 0.0 9.393199409846213 7.907092923286254E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 27850 0.0 55.287605 1 TACCCCG 635 0.0 50.9756 5 GTATCAA 55645 0.0 43.3579 1 ACCTGGG 2160 0.0 40.623875 3 TCAAGAC 1675 0.0 40.060352 3 TACCTGG 2475 0.0 36.20806 2 CCCGGCG 895 0.0 35.11845 8 TCTCTGC 7590 0.0 35.10671 8 CAACGCA 66580 0.0 35.053898 5 ATCAACG 67405 0.0 34.612465 3 TCCGCAC 455 0.0 34.032673 3 TATTCTC 7925 0.0 33.800556 5 CCGCACA 465 0.0 33.300655 4 TATCAAC 73105 0.0 32.731827 2 AACGCAG 75250 0.0 31.875198 6 TATCCGC 355 0.0 31.727047 1 GTACTAG 10475 0.0 31.405867 1 TAGGCAT 13170 0.0 30.09941 5 TTCTCTG 8970 0.0 29.653381 7 CATGGGT 3595 0.0 29.368073 4 >>END_MODULE